Method for simulating hip dislocation

FIELD: medicine.

SUBSTANCE: originally, epiphysial hip dysplasia is formed in 10-day-old golden hamsters with projection of the joint exposed to local infra-red pulse laser light daily for 20 days. Then, on the following day, simple hip dislocation of an experimental extremity of a hamster is performed by gradual extension of a capsular-ligamentous apparatus of the hip joint. Ventral and dorsal zones of a joint space are subject to mechanical vibrations by a border of a thin metal plate fixed in a stably fixed vibrator. The following parameters are used: joint capsule pressure 1 kg, vibration frequency 100 Hz, amplitude 0.3 mm for 15 minutes to the moment of femoral head exit from an acetabular cavity. Then, the femoral head is displaced manually behind an external surface of the cavity. In 2 days, the hamster is laid on its back, and the experimental hip is gradually taken aside from a vertical plane to a position of an apex of the head of the hip joint in a horizontal plane. The procedure is performed daily for at least 3 times for 7 days.

EFFECT: use of the given invention allows performing sparing simulation of the simple hip dislocation similar to a mechanism of hip dislocation pathogenesis in children that testifies to possibility of the use of this model for studying macro- and microscopic changes in the ends of the bones formed during growth.

5 dwg, 4 ex

 

The present invention relates to medicine, namely to orthopedics, and can be used for simulation of closed dislocation of the hip.

The study of malformations of the hip joint in growing individuals will serve as the basis for the development of methods of treatment of congenital dislocation of the hip in men and the prevention of its development /1-3, 4/.

There is a method of modeling anomalies femoral and pelvic bones by crossing branches of the femoral and acetabular artery to the ingrowth of blood vessels of the round ligament to the head of the femur /5/. However, this method has a fundamental difference from proposed, which consists in the fact that it runs through the open, for which you have all the disadvantages of surgical interventions: dissection of soft tissue, open violation of the safe growing intraosseous vessels, hemorrhage, need for closing wounds, etc. which is not observed in the pathogenesis of congenital dislocation of the hip in children of man.

Closest to the proposed method closed dislocation of the hip is a method of modeling the epiphyseal dysplasia, carried out by laser radiation, inhibiting the growth of intraosseous vessels and thereby causing the later formation of bone cores in the epiphyses of bones that form the joints /6/. This method of growth disorders of the blood vessels on the pathogen the memory corresponds to the slow development of intraosseous blood vessels and the formation of bone cores in the proximal epiphysis of the femur in the initial period of formation of congenital dislocation of the hip in humans, namely, in the first stage of the disease, called dysplasia, which leads then to the decentration of the femoral head relative to the acetabular socket /7/.

However, the known methods have significant drawbacks, since laser irradiation growing hip joint is not possible to obtain, at the modern level of development of laser devices, stages II and III closed dislocation of the hip, i.e. lateralization (subluxation and dislocation of the head. This is due to the fact that these abnormalities in the development of tissues in the ends of the bones forming the hip joint, and further does not lead to their anatomical deformation, because there is no mechanical load on the head of the femur due to the fact that the animal spares irradiated limb, transferring the load of the body weight on the other three limbs, and thereby prevents the possibility of development are characteristic for the closed hip dislocation symptoms: tension and increase capsular ligament of the joint /8/.

Based on the fact that the epiphyseal dysplasia is the initial stage in the development of closed dislocation of the hip, the pathogenesis of which corresponds to the development of further stages of closed dislocation of the hip, the task was: to obtain the model of dislocation of the hip in mammals, closest to the pathogenesis of congenital dislocation of the hip in humans conservative closed the th path.

The set task is solved as follows. Modeling of dislocation of the hip includes the initial formation of epiphyseal dysplasia of the hip joint in 10-day Golden hamsters local irradiation of the infrared pulse laser radiation of the projection of the joint daily for 20 days. New in the solution of the problem is that after laser irradiation and receiving epiphyseal dysplasia of the hip the next day, closed produce hip dislocation experienced limb Golden hamster by gradual stretching of the capsular ligament of the hip joint, the effects of mechanical vibrations on the ventral and dorsal areas of the joint space joint edge of a thin metal plate, fixed in a stable fixed vibrator pressure on the capsule of the joint force of 1 kg, the oscillation frequency of 100 Hz, the amplitude of 0.3 mm for 15 minutes until the femoral head from the acetabular cavity, then manual displace the head of the femur over the outer surface of the hollow, then after 2 days Golden hamster laid on your back and gradually divert experienced the thigh from the vertical plane to the location of the tip of the hip joint in the horizontal plane every day at least 3 times in 7 days.

Just to clarify things is to promote the distinctive features of the proposed Method simulation of dislocation of the hip".

Simulation of closed dislocation of the hip will be performed by irradiating infrared pulse laser radiation of the skin surface in the area of the hip joint 10-day Golden hamsters daily for 20 days at a radiation power of 10 mW, exposure for 10 minutes, the wavelength of 0.9 μm and the pulse frequency of 2500 Hz. This allows you to delay the development of bone cores, and means to prevent the increase of mechanical strength increasing femoral head, which facilitates its release from the acetabular cavity in the preservation of the elasticity of the cartilage of the articular surfaces when exposed to mechanical vibrations. The application of laser radiation before using vibration contributes to a more active movement of the femoral head from the trough, so as irradiation, restraining the growth of blood vessels and bone tissue, does not affect the development of the cartilaginous mass of the femoral head, and less Mature cartilage is softer and more elastic, less resists when moving the head from the basin by reducing friction between the articular surfaces of the bones.

However, completed within 80 days of laser irradiation of femoral head bone hamsters, showing the slow replacement of cartilage by bone in the area metapersonal germ plate, however, was not accompanied by the onset of podwave the and or dislocation of the hip, that leads to opportunities.

The next day after completion of the laser irradiation and receiving epiphyseal dysplasia of the hip, closed produce hip dislocation experienced limb Golden hamster by gradual stretching of the capsular ligament of the hip joint, the effects of mechanical vibrations on the ventral and dorsal areas of the joint space joint edge of a thin metal plate, fixed in a stable fixed vibrator pressure on the capsule of the joint force of 1 kg, the oscillation frequency of 100 Hz, the amplitude of 0.3 mm for 15 minutes until the femoral head from the acetabular cavity, which allows to achieve the project objectives was to create a model of dislocation of the hip. The use of mechanical vibration using a vibration apparatus for subluxation and dislocation of the femoral head, passed through the soft tissue on the articular surface depression and the femoral head, contribute to their divergence due to stretching of collagen and elastic fibers of the connective tissue of the capsule-ligament apparatus, providing an increase in the capsule of the hip joint and the length of the ligaments. The choice of applying mechanical vibrations (vibration), as a physical factor modeling of dislocation of the hip, a reasonable possibility is updated on receipt of hip dislocation closed by. Fluctuations contribute to the reduction of mechanical stresses soft tissue accompanying dislocation of the hip when stretching, along with pressure on the articular surfaces of bones helps them to move relative to each other and reduce the degree of traumatization of both soft tissues and articular surfaces of the ends of the bones and this ultimately allows for a short time to obtain the private dislocation of the hip. Manual displacement of the femoral head for the outer surface of the acetabular cavity allows gentle atraumatic technique to obtain closed dislocation of the hip.

Exercise 2 days after receipt of the hip dislocation laying Golden hamster on your back and perform a gradual discharge of experienced hip from the vertical plane to the location of the tip of the hip joint in the horizontal plane every day at least 3 times in 7 days ensures lasting hip dislocation. Since the proposed method of obtaining traumatic hip dislocation, after its execution intact muscles will seek to samoupravlenie of the femoral head into the cavity of the basin, as observed in the growing children of man 191. To avoid this, two days after receipt of the dislocation carry out preventive measures in the form of leads hips a daily basis that promotes the juice is asenio amount of depression and the development of a new depression in the area of the new location of the femoral head on the surface region of a limb or the outer surface of the wall of the trench.

Conducted patent research division A61N 5/06, G09B 23/28, A61H 23/00 and analysis of scientific and medical information that reflects the current level of modeling techniques of hip dislocation is not revealed identical technologies. Thus, the proposed method simulation of dislocation of the hip is new.

The relationship and interaction of the essential techniques of the proposed method of obtaining a model of a hip dislocation achieve a new result in the solution of the problem, namely:

to obtain the model of closed dislocation of the hip in mammals closest to the pathogenesis of congenital dislocation of the hip in humans conservative closed by. Thus, the proposed method involves an inventive step.

The proposed method simulation of dislocation of the hip can be widely applied in experimental medicine to study the pathogenesis of congenital dislocation of the hip in children of man and the development of methods of pathogenetic treatment of this disease. Perhaps his repeated playback in the experimental laboratory.

The essence of the proposed method is illustrated by the following illustrations:

1,and presents the control epiphysis (head) of the femur, in which visible vascular lesions (arrow)lying in the cartilage tissue, the term of experience 25 days;

Figure 1,b on OBrien experienced epiphysis, in cartilage tissue which vessels are not available, the term of experience 25 days;

Figure 2,a - visible epiphysis (head) of the control of the femur, where the cartilage replaced by bone tissue (arrow), the term of experience of 30 days;

2,b - presents the epiphysis experienced the femur, which has the appearance of blood vessels and the beginning of bone formation in the cartilage tissue (arrow), the term of experience of 30 days;

3,and is presented to control the hip joint, consisting of acetabular cavity (C) and adjacent to it the head, the life experience of 45 days:

Figure 3,b shows experimental hip joint, consisting of the former basin (b) and new emerging basin (g), which is adjacent the head of the femur, the term of experience 45 days;

Figure 4,a - term experience 60 days - presents control the hip joint, consisting of acetabular hollows (in) and its adjacent head;

Figure 4,b - term experience 60 days, presents experienced hip joint, consisting of former acetabular cavity (C) and new emerging basin (g), which is adjacent the head of the femur;

Figure 5,a - term experience 90 days, control the hip joint and the first cavity ();

Figure 5,b - term experience of 90 days experienced hip joint, remains basin (in) and the newly formed cavity (g).

The essence of the Method is the modeling of dislocation of the hip" is the following. Modeling closed dislocation of the hip in Golden hamsters precedes the receipt of the epiphyseal dysplasia, which is the stage I of congenital dislocation of the hip resulting from laser irradiation of the skin of the hip joint 10-day Golden hamsters during 20 days /6/.

Laboratory Golden hamsters on the 11th day after birth is placed in the palm of the left hand and the right take the working end of the fiber and africasouth with the surface of the skin of the hip joint so that it is relative to the neck of the femur is positioned at an angle of 45-90° to the horizontal and sagittal planes, which makes it possible to irradiate the blood vessels of the neck and the femoral head in animals. In a predetermined position of the infrared pulse laser power of 10 mW, a pulse frequency of 2500 Hz, the wavelength of 0.9 μm is irradiated with the hip joint for 10 minutes. The next day after the end of the sessions of laser irradiation and receiving epiphyseal dysplasia of the hip carry out the modeling stages II and III closed dislocation of the hip. By this time the body weight 30-day hamsters is about 50 grams. Under General anesthesia by injecting intraperitoneally, for example, 0.25 ml of a solution of ketamine (given the volume of solution taken based on the fact that anesthesia is required 50 mg of the substance per 1 kg mass telehomecare) experimental animal take in the palm of his left hand, and right away experienced hind limbs and closed produce hip dislocation experienced limb Golden hamster. Hip dislocation is obtained by gradual stretching of the capsular ligament of the hip joint, the effects of mechanical vibrations on the ventral and dorsal areas of the joint space joint. Mechanical vibrations provide using stably fixed vibrator with working part made in the form of a thin metal plate, the edge of which carry the pressure on the joint capsule in the inguinal folds force of 1 kg for 15 minutes until the femoral head from the acetabular cavity. The oscillation frequency of the working part of the vibrator is 100 Hz, the amplitude of 0.3 mm, While the body of the hamster and experienced thigh support arms are located in a horizontal plane so that the edge of a thin metal plate was sent to the place of articulation of the edge of the basin and the femoral head. Then manual displace the head of the femur over the outer surface of the basin. While retaining hamster in the left hand and the right exercise pressure on the end of the femur through the end of the bent leg from the bottom up, thigh placed at an angle of 90° to the long axis of the body. The left brush creates a counter pressure on the pelvis from above. These movements reach the possibility of dislocation of the hip upward,aided distended capsular ligaments of the hip joint. Monitoring dislocation of the hip is carried out by contact of the thumb of the right hand with spit or head of the femur. Pressure up exercise gradually, with increasing force, which must not be accompanied by rupture of the capsular ligament and muscles, but only to contribute to their strength. Offset head for the outer edge of the acetabular cavity is accompanied by the symptom of "click". After receiving the effect of the easy mobility of the head when it is dislocated, you should verify this by creating opportunities to make a circular movement of the head along the perimeter of the limb. Upon receipt of the hip dislocation animal is placed in an empty cell until the moment when it will be able to move independently. This period is 1-2 hours after giving anesthesia and then hamster is transferred into the cell to the mother. 2 days after receipt of the hip dislocation perform hip abduction experienced extremity of the vertical plane to the location of the top of the head in the horizontal plane at the position of the hamster on the back. Exercises are conducted daily at least 3 times in 7 days. Control the output of the femoral head from the basin by its tenderness in the groin area, followed by symptom "click". As a rule, the condition of the animals when this is satisfactory, they get used to these exercises and hip dislocation persists, as the head of his pressure creates a new socket. In this period of experience hamsters transported from the mother. Subsequent removal of the animals from experience in the given time carry pairs of ether. After euthanasia see pelvic girdle with legs. Spend an anatomical preparation of the soft tissues with the study and description of the condition of the pelvis thigh.

To identify state changes of the bones of the hip after dislocation of the hip using mechanical vibrations with a frequency of 100 Hz, which is the maximum amount that is not having a harmful effect on man /10/, experiments were carried out on 12 hamsters aged 10 days after birth.

The results of the study of anatomical preparations of the hip joints are explained with examples of their study in terms of 45, 60 and 90 days after birth and 15, 30, 60 days after dislocation of the hip. The essence of the proposed simulation method is illustrated by examples:

Example No. 1.

Simulation of closed dislocation of the hip performed at stage I, which consists in obtaining the epiphyseal dysplasia by irradiation of infrared pulsed laser radiation skin surface 10-day Golden hamsters.

Experimental hamster on the 11th day after birth take in the palm of the left hand and the right hand take the end of the fiber and africasouth it with the skin surface of the left hip joint project in which the neck of the femur at an angle of 60° to the horizontal and sagittal planes. In a predetermined position of the infrared pulse laser power of 10 mW, a pulse frequency of 2500 Hz, the wavelength of 0.9 μm hip joint is subjected to irradiation for 10 minutes. Then take to experience the following animal by placing in the previous cell to the mother. Laser treatment was performed daily for 20 days. On sections of histological control of the epiphysis 25 days visible vessels (see the Appendix for the description of figure 1,a) and 30 days of a beam bone cores (see the Appendix for the description of figure 2,a (arrow). Accordingly irradiated on the epiphyses of experimental animals vessels or missing (see the Appendix for the description of figure 1,b), or marked the beginning of their appearance (see the Appendix for the description of figure 2,b (arrow).

20 days after the termination of irradiation shall modeling stages II and III closed dislocation of the hip. By this time the age of the hamsters is 30 days. Under General anesthesia by intraperitoneal injection of 0.25 ml of ketamine in experimental hamster take in the palm of the left hand, right away the left rear leg of the hamster and received in the space between the body and the designated thigh is placed a thin metal plate constituting the working part rigidly fixed to the vibrator so that its thin rounded edge rested in the groin warehouse is the skin. While the body of the hamster and left thigh hold hands and placed in the horizontal plane so that the edge of the metal plate was sent to the place of articulation of the depression and the femoral head. Includes the vibrator and the experimenter creates a craving for his body and the distal end of the left hip for the advancement of hip and pelvis hamster to himself with a tension of 1 kg, which leads to the pressure thin rounded edges of the metal plate at the bottom of the hip joint. Simultaneously, the middle finger of the left hand create support for tuberosity ischial bone, thus preventing rupture of the left sacroiliac joint in the form of synchondrosis. The thumb of the right hand to create pressure on the inner surface of the left thigh and with this and forefinger grasp the distal end of the femur above namyslow and create cravings hip diverting his head laterally. Thereby carry out a movement that promotes stretching of the capsular ligament of the left hip joint. In this position the body of the animal is affected by vibration with a frequency of 100 Hz, the amplitude of 0.3 mm, when the power pull 1 kg in a period of 7.5 minutes. Then hamster turn back to the thin edge of the surface of the metal plate of the vibrator and create pressure in the projection slit thatbetter the joint traction pelvis and hips in the projection between the limb and the greater trochanter of maintaining the pressure with the fingers on the ischial tuberosity bones and inner thighs with his abduction. These movements contribute to the stretching of the capsule-ligament apparatus with the dorsal side of the hip joint, produced in a period of 7.5 minutes. Turn off the vibrator. Then, holding the hamster in the left hand, the right exercise pressure on the end of the thigh from the bottom up, with the hip placed at an angle of 90° to the long axis of the body. The left brush creates a counter pressure on the pelvis from above. These movements reach dislocation of the hip upward, aided distended capsular-ligamentous apparatus. Pressure up exercise gradually, with increasing force, which should not lead to rupture of the capsular ligament and muscles, but only to contribute to their strength. Monitoring of dislocation is carried out by contact of the thumb of the right hand with the greater trochanter or the head of the femur. When the impossibility of motion of the hips up, his distal end to bring down the bottom end of the limb to obtain dislocation. At the same time to prevent epiphysiolysis should capture hips above namyslow. Simultaneously carry out the abduction of the thigh to the horizontal plane, which contributes to the dislocation of the hip, thus control the motion of the greater trochanter through the touch of a finger. Transition head for the outer surface of the depression is accompanied by symptom "click"that tells the offset of the femoral head from the acetabular cavity for the outer surface of the basin. A large skewer goes out from under your finger down and in place of articulation of the depression and the head feels weak deepening. Then, using the diaphysis of the femur as a lever, repeat the movement of the femoral head down, up, it lead to the onset of dislocation in any point of the limb, which is the prevention of the reduction of the head into the cavity after the release hamster from anesthesia. Ensuring the achievement of hip dislocation, the animal is placed before his recovery from anesthesia in an empty cell, and after recovery from anesthesia can be translated into the cage for the mother or be placed in a common group of animals with a dislocated hip for the continuation of the experiment. 2 days after execution of hip dislocation begin to implement the abstraction experienced hips to prevent his samoupravleniya. Hamster laid on the back and the diaphysis experienced hip gradually divert to the horizontal plane, controlling thumb of the time the head of the acetabular cavity, or finding it on the edge or on the outer surface of the basin. Repeat this exercise daily at least 3 times in 7 days.

At the end of the process of prevention of possible samoupravleniya sprained femoral head animals were monitored during the growth period of their skeleton, which is 3 months. Within the specified time frame of the experiments conducted their euthanasia pairs of ether, after which he performed the amputation of the pelvic girdle and then performed an anatomical preparation with the release of the soft tissues of the pelvis and femur.

7 days after the end of the simulation epiphyseal dysplasia and prevention samoupravleniya sprained femoral head was performed euthanasia hamster pairs of ether. Then performed an anatomical preparation of the pelvis and hips. At autopsy experienced hip joint, the femoral head lies partially on the surface of the edge wall of the acetabular cavity, pushing the fabric of the limb beyond its front upper surface. The trough is empty, its diameter is 2.8 mm Front-top edge of the acetabular cavity slightly deformed and thickened due to pressure of the femoral head, its diameter equal to 2.5 mm At the top of the head is visible to the end of the remainder of the round ligament. Haemorrhage in the cavity of the basin and its surrounding soft tissues were not detected.

In the control the hip joint at the opening of the capsule, the head of the femur lies in the basin. During insertion of the head of the basin is marked preservation of the round ligament. The diameter of the control head is 2.8 mm, the length of the thighs - 20 mm

Example No. 2.

Manipulations performed in the same way as in example No. 1.

45 days after the birth of a hamster and his euthanasia at the opening of the capsule control of hip joint, the head of the femur lies in the basin. During insertion of the head of the basin is marked preservation of the round ligament (see the Appendix for the description of figure 3,a). Head diameter equal to 2.8 mm At uncritically experienced hip joint, the femoral head diameter of 2.8 mm (see application to the description of Fig 3,b) lies beyond the outer surface of the front bottom wall of the trench, which is formed adjacent the bottom of the new depression of hyaline cartilage (see the Appendix for the description of figure 3,b-d). The upper back wall of the still basin deformed under the pressure of the femoral head. Herself depression presents a narrow slit area of 1×0.8 mm, running obliquely in the front-upper direction (see the Appendix for the description of figure 3,b-in). The head of the femur has a diameter of 2.8 mm and not contains the round ligament.

Thus, within 15 days after the front-lower dislocation of left hip, the experienced head of the femur through the pressure generates a new depression, and the depression is subjected to deformation, underdevelopment and decrease in volume. Dice control of hip joints develop normally, indicating that there was no influence of vibration affecting the bones of the same name experienced joint.

Example No. 3.

Manipulations performed in the same way as in example No. 1. When the anatomical study of the hip joints 60 days after the birth of a hamster and his euthanasia at the opening of the capsule control of hip joint, the head of the femur lies in the basin (see the Appendix for the description of figure 4,a-C). Its diameter of about 2.8 mm and a depth of 1.4 mm In the case of removing the head from depression marked the preservation of its round ligament. The diameter control is Noah head 2,8 mm

At the opening of the stretched capsule experienced hip joint, the femoral head lies beyond the outer surface of the wall of the acetabular cavity, which is formed adjacent the bottom of the new depression (see the Appendix for the description of figure 4,b-d). The upper back wall of the still basin deformed under the pressure of the femoral head. Herself depression presents a narrow slit area of 0.3×2 mm and a depth of 1.4 mm, running obliquely in the front-upper direction (see the Appendix for the description of figure 4,b-in). Round ligament on the top of the head is missing. Head diameter 2 mm.

Thus, within 30 days after hip dislocation experienced head of the femur remains located over the outer surface of the wall of the acetabular cavity and its pressure continues to build the inner surface of a new depression. Still the depression continues to undergo deformation, underdeveloped and decreases in volume. Dice control eponymous hip continue their normal development.

Example No. 4. Manipulations performed in the same way as in example No. 1.

When the anatomical study of the hip joints 90 days after birth hamster and euthanasia at the opening of the capsule control of hip joint, the head of the femur (see the Appendix for the description of figure 5,a) lies in a developed basin (see attached the e to the description of figure 5,a-C). Her depth of 2 mm In the case of removing the head of the basin is marked preservation of the round ligament. The diameter of the control head 3 mm.

At the opening of the stretched capsule experienced hip joint, the femoral head (see the Appendix for the description of figure 5,b) lies in the newly formed depression (see the Appendix for the description of figure 5,b-d) depth of 0.5 mm, which was formed around the former basin (see the Appendix for the description of figure 5,6), presents a narrow slit area of 0.5×to 0.1 mm, the Diameter of experienced heads 3 mm, it has a rounded shape.

At the end of skeletal growth hamster obtained experimentally closed dislocation of the hip is maintained. Experienced head does not have the round ligament, reduced in diameter by 0.3 mm and lies in the newly formed depression about still subjected to reverse development. Capsule experienced joint is stretched. The state experienced a hip joint in a given period of observation suggests that in the next samoupravlenie experienced the femoral head is impossible and it is possible to conclude that the termination of further experiments on the formation of closed dislocation of the hip in Golden hamsters.

Thus, the Method of modeling of dislocation of the hip allows closed gentle way to get in experiment dislocation of the hip by anatomical changes of the elements of hip sousta is rather similar to the mechanism of pathogenesis of hip dislocation in children of man, that suggests the possibility of using this model to study the macro - and microscopic changes in the ends of the bones that are formed during the growth of the animals to develop measures for the treatment and prevention of abnormally developing hip joints in children of man.

Sources of information

1. Wolves MB Bone pathology in children. - M.: Medicine, 1968.- 496 C.

2. Lagunova I.G. Clinical and radiographic diagnosis of skeletal dysplasia. - M.: Medicine, 1989. - 256 S.

3. Reinberg S.A. X-ray diagnosis of diseases of bones and joints. - M.: Medicine, 1964. - 530 S.

4. Sharper E Dynamics of developmental disorders of the hip in children and adolescents. System surgical treatment and rehabilitation. Abstract... Prof. the honey. Sciences. - M., 2004. - 37 S.

5. Pereklicka PF Way of modeling anomalies femoral and pelvic bones. RF patent №2279144, 2006, bull. 18.

6. Pereklicka PF Way of modeling the epiphyseal dysplasia. RF patent №2340952, 2008, bull. 34.

7. Traumatology and Orthopaedics. A guide for physicians. /Under. edit Navarinou, Aggression./ - SPb.: Hippocrates, 2006.- V.3. - S-986.

8. Frejka B. Congenital dislocation of the hip. Manual of Orthopaedics and traumatology. - M.: Medicine, 1968.- SO - S-616.

9. Marx V.O. Congenital dislocation of the hip in newborns. / Orthopedist. traumatol., 1939. - CN. - P.23-32.

10. Kilinc the A.K., Veretennikov P.D., Archilab M.A. the Effect of vibro-acoustic factors of the labour process on the body of the worker. / The Bulletin of the Altai state Agram. University, 2007, - №7(33). - P.54-58.

The method of modeling of dislocation of the hip, including the initial formation of epiphyseal dysplasia of the hip joint in 10-day Golden hamsters local irradiation of the infrared pulse laser radiation of the projection of the joint daily for 20 days, wherein after irradiation and receiving epiphyseal dysplasia of the hip the next day, closed produce hip dislocation experienced limb Golden hamster by gradual stretching of the capsular ligament of the hip joint, the effects of mechanical vibrations on the ventral and dorsal areas of the joint space by the edge of a thin metal plate, fixed in a stable fixed vibrator, the pressure on the capsule of the joint force of 1 kg, the oscillation frequency of 100 Hz, the amplitude of 0.3 mm for 15 min until the femoral head from the acetabular cavity, then manual displace the head of the femur over the outer surface of the basin, and then 2 days Golden hamster laid on your back and gradually divert experienced the thigh from the vertical plane to the location of the tip of the hip joint in the horizontal the orbital plane every day at least 3 times in 7 days.



 

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5 dwg, 4 tbl

FIELD: medicine.

SUBSTANCE: venous ischemia is formed by bipolar precision coagulation of venous vessels ending by a superior sagittal sinus on the area 1 cm2. It is followed by obliteration of a middle third of the sagittal sine by bipolar coagulation.

EFFECT: method extends the range of products for simulating ischemic cerebral affection.

1 ex

FIELD: medicine.

SUBSTANCE: skin is incised, and wound edges are anchored to subjacent tissues. Each wound angle is underrun additionally with a separate diagonal suture. A formed scab and necrotising tissues are excised within healthy tissues. Then the wound is treated with an antiseptic and washed with physiologic saline. Thereafter, the treated wound surface is coated with a semipermeable membrane under the angular diagonal sutures to introduce a bacterial suspension containing 0.5 ml E.coli 109 and 0.5 ml Ps.aeraginosa 109.

EFFECT: method provides a model of the infected skin wound with preset bacterial population with its characteristics as much as possible approached to an actual clinical course of the wound process.

3 tbl, 7 dwg

FIELD: medicine.

SUBSTANCE: wound is irrigated with a metabolite produced by growth of Bacillus subtilis 804 strain bacteria in an amount 1 ml in 1 cm2. A transplant is placed.

EFFECT: method provides enhanced transplant adhesion, reduced risk of lysis.

2 ex

FIELD: medicine, experimental abdominal surgery.

SUBSTANCE: as experimental animals one should apply mongrel dogs of 12-17 kg body weight. Under general anesthesia one should conduct superior-median laparotomy, introduce 3.0 ml 70%-ethanol solution under pancreatic capsule and then laparotomic wound should be sutured up. Manipulation should be performed once. The method provides modeling adequate acute pancreatic inflammation at no side effects being very simple in implementation.

EFFECT: higher efficiency.

3 dwg

FIELD: medicine; medical engineering.

SUBSTANCE: method involves studying transverse longitudinal and rotation stiffness characteristics. The studies are carried out step-by-step from the first order units to complete external fixation apparatus structure. The device has frame and is provided with calibration loads, wire rope, displacement indicators, strip for fastening to loading end of bone imitator fragment, beam for fixing displacement indicators, beams having unit for modeling longitudinal and transverse loadings. The frame is manufactured as parallelepiped. The fixing panel has openings for bone imitator, for fixing external fixation apparatus and yoke connection union and is fixed in end face part of the frame. Beam for fixing displacement indicators has longitudinal slit for fixing the indicators and arranging them on lateral slots in frame base. The beams having unit for modeling rotational, longitudinal and transverse loadings are arranged on lateral frame sides on lateral slots in base.

EFFECT: high vision acuity without applying spectacle-based correction; accelerated treatment course.

2 cl, 16 dwg, 1 tbl

FIELD: experimental medicine.

SUBSTANCE: the present innovation deals with modeling urinary calculosis in rats due to injecting intraperitoneally 60%-glucose solution at 1 ml/100 g animal body weight twice daily for 2 mo. The method is very simple and enables to achieve lithogenesis in 25% experimental animals.

EFFECT: higher efficiency of experimental modeling.

2 dwg, 2 ex, 4 tbl

FIELD: medicine.

SUBSTANCE: method involves using Hann diode crystal with proper frequencies of pathogenic microorganisms and cells during their death period or during the stimulating factors action period being applied.

EFFECT: enhanced effectiveness of treatment; wider range of biophysical action types.

3 cl, 1 tbl

FIELD: medicine, experimental physiology.

SUBSTANCE: hypoxia with hypercapnia should be modeled due to creating a closed system of inhaled air circulation. Air enters lungs out of hermetically sealed reservoir and at expiration returns back. The process of recirculation is supplied with an apparatus of artificial pulmonary ventilation. The innovation suggested provides steadiness in development of hypoxia with hypercapnia excluding the development of stressor reaction. Conditions should be created to carry out any manipulations with an animal in the course of an experiment.

EFFECT: higher efficiency of modeling.

5 dwg, 1 ex

FIELD: medicine, stomatology.

SUBSTANCE: the present innovation should be carried out for the purpose to study ethiology and pathogenesis of parodontitis. One should affect with emotional stress in experimental animals (mature rats) due to placing 10-11 experimental animals into the cage at area of 0.018 sq. cm/animal. Before placing into the cage one should create artificial dental plaque around the cervix of the upper and lower incisors with the help of stomatological cement for every experimental animal. In the course of modeling all experimental animals should eat paste-like food. The method enables to shorten terms for obtaining the model desired and increase its similarity with pathomorphological manifestations of human parodontitis.

EFFECT: higher efficiency of investigation.

2 ex

FIELD: medical equipment.

SUBSTANCE: device has input first variable resistor, capacitor and permanent resistor. Permanent resistor is connected to arm second and third variable resistors. Second ends of variable resistors are connected with motionless contacts of polarized relay. Movable contact of relay is connected to common bus. Input of device is connected to amplifier which has output connected with control wiring of polarized relay. Second end of wiring is connected with common bus. Device is intended for electrical modeling of balanced and misbalanced conditions of acupuncture point at electropunctural action with unlike-poled signals due to liquidation mutual errors at any circuit of opposite arms of the device. Values of active resistances can be installed independently at any arm of device.

EFFECT: increased precision.

2 dwg

FIELD: experimental medicine.

SUBSTANCE: laboratory animals should be once injected intraperitoneally or intravenously with phenylhydrazine at the dosage of 100-150 mg/kg.

EFFECT: higher efficiency.

1 cl, 4 ex, 2 tbl

FIELD: medicine, experimental biology, ecology, toxicology.

SUBSTANCE: at studying the mechanisms of heavy metals toxic action, in particular, cadmium upon renal function, it is suggested to introduce cadmium sulfate solution into stomach once daily for 2 mo at the dosage of 0.5 mg/kg, on conversion to metal, where cadmium corresponds to 0.5 mg per 1 ml solution. The present innovation enables to study the pathology in dynamics of development and elaborate and searching preparations for treating and preventing chronic toxic nephropathy.

EFFECT: higher efficiency.

1 ex, 3 tbl

FIELD: medicine.

SUBSTANCE: method involves exposing cell or cell group to external power source. At least two electrodes are introduced before treating the cells. One of electrodes is set on cytoplasmatic external cell membrane surface and the other one cell membrane and membrane potential value is measured. External electric voltage source is connected to the introduced electrodes oppositely in polarity with cell membrane potential difference value being not less than cell membrane potential.

EFFECT: enhanced effectiveness in building cell damage model by means of energy burst and death.

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