Method of extracting and stabilising low-molecular aminoglycans from eggshell wastes

FIELD: chemistry.

SUBSTANCE: invention relates to a method of extracting and stabilising ultra low-molecular aminoglycans from eggshell wastes. Aminoglycan extract is used to produce cosmetic creams with skin moisturising and anti-wrinkle properties. The method of extracting low-molecular aminoglycan compound of formula I from a natural source of eggshell wastes, which consists of alternating glucuronic acid and N-acetylglucosamine units, where M can be one or more of Na, Ca, K, Mg; and n is a whole number from 20 to 40, involves the following steps: (a) preparing eggshell wastes for extraction of embryonic low-molecular aminoglycan compound of formula I using a polar organic solvent in water, (b) extracting low-molecular aminoglycan compound of formula I in form of a water-soluble salt, for which the eggshell from step (a) is vigorously shaken with aqueous polar salt solution at 10C - 35C for 6-12 hours, then filtered or centrifuged in order to collect an aqueous layer containing a dissolved aminoglycan compound of formula I; (c) extracting a purified low-molecular aminoglycan compound of formula I by forming a gel from an aqueous mixture of salts using a polar organic solvent, for which the solution from step (b) is successively and step-by-step mixed with an organic solvent mixed with water while gently stirring and then cooled to maintain temperature from 20C to 25C, and the formed gel is left for 2-24 hours for complete precipitation, then filtered or centrifuged in order to extract a semidry aminoglycan compound of formula I; (d) the extracted aminoglycan compound of formula I from step (c) is stabilised via gradual addition of organic oils to the semidry gel to form aminoglycan compound of formula I. In order to prepare a composition having anti-wrinkle properties, at least one pharmaceutically acceptable filler is added to the stabilised aminoglycan compound of formula I obtained at step (d).

EFFECT: method enables to obtain an aminoglycan compound of formula I with the necessary viscosity and skin penetrating properties for reducing skin wrinkles, as well as excellent softening and moisturising effects.

8 cl, 9 ex

 

The scope of the invention

Embodiments of the present invention relate to method a simple and effective allocation and stabilization ultrametabolism aminoglycan from waste egg shell.

Prior art

Embodiments of this invention relate to methods of allocation, stabilization and formulation of low-molecular aminoglycan from waste egg shell. Extract aminoglycan used for the manufacture of cosmetic creams with properties moisturizing and anti-wrinkle.

Nakano and others (Poult Sci. (1991), Vol.70 (12), pp.2524-8) showed that the chemical composition glycosaminoglycans fractions from crest and beard in males of the white Leghorn breed with foliate crest consists of glycosaminoglycans with a very large molecular weight, which are used in therapy of replacement of cartilage.

Balazs and others (U.S. patent No. 4141973) described a method of separating pure hyaluronic acid from tissue of animals having a molecular weight in the range of 1 MD (molecular weight) - 6 MD-used as a substitute for synovial fluid and the vitreous body.

Heaney and others (Biochim Biophys Acta. (1976), Vol.18; 451 (1), pp.133-42) showed that the organic part of the shell of eggs consists of collagen, proteins and polysaccharides, which are probably present in the form of glycoproteins and glycosaminoglycans. Then, at the same time chromatography was determined organic components for the production of glycosaminoglycans, having a minimum molecular weight of 30,000 daltons. Analysis of the deposition rate in the density gradient showed that the polysaccharides contained equimolar amounts of glucosamine (36,3% share) and glucuronic acid 35.6% wt./weight. Identification of degradation products showed that glycosaminoglycan mainly was hyaluronic acid.

Stahl and others (U.S. patent No. 6537795) described a method of obtaining and allocating aminoglycan from cultivated strains of streptococcal fermentation. Data aminoglycan are characterized by their extremely high molecular weights up to 6 MD and applied in therapy of replacement of cartilage.

Such methods of isolation and purification of glycosaminoglycans from other natural sources and animal tissues also can be found in U.S. patent No. 5824658, U.S. patent No. 6660853, U.S. patent No. 6451326. The materials that were discussed in these patents included in the description of this invention by reference.

Brief description of the invention

Embodiments of the present invention to provide a new method of extraction of low-molecular aminoglycan the compounds of formula I, which consists of alternative glucuronic acid and N elements-acetylglucosamine of previously unknown natural source of waste egg shell,

where M can be one or the more of Na, Ca, K, Mg; n is an integer from 20 to 40.

Moreover, the method includes the steps are:

(a) pre-prepared waste egg shell to extract embryonic molecular aminoglycan the compounds of formula I, using a polar organic solvent dissolved in water

(b) extracted low-molecular aminoglycoside compound of formula I in the form of its water-soluble salt, using aqueous polar saline,

(c) produce a purified low molecular weight aminoglycoside compound of formula I forming a gel from an aqueous mixture of salts, using a polar organic solvent followed by filtration and centrifugation,

(d) stabilize the selected extract aminoglycan gradual introduction of organic oils in semi-dry gel formation aminophenolic compounds of formula I.

Embodiments of the present invention more specifically relate to the step (b), where the polar water salt solution may be the salt of citrate, glutamate, acetate, pyrrolidinecarbonyl, tartrate, glycinate, sulfate, sulfite, nitrate, carbonate or oxalate, sodium, potassium, calcium or magnesium to a solution containing aminoglycoside the compounds of formula I that are acceptable for selective gelatinization and selection.

The described method I have is a new method for selective and easier to get a low-molecular aminophenolic compounds of the formula I from waste egg shell. More specifically, the method according to this invention in comparison with the methods of allocation aminoglycan, disclosed in the prior art, characterized in that:

a) is identified as a new one that uses a previously unused source, the waste egg shell, which is otherwise difficult to get rid of and which cause significant harmful impact on the environment

b) contains a very low concentration of harmful proteins and nucleotides,

c) does not require any expensive and inefficient separation of organic and inorganic materials in the waste egg shell,

(d) includes more than simple extraction using mild reagents and solvent materials, and

e) does not require acetylation or other derivatives, for example, using acetic anhydride and sulfuric acid as described in U.S. patent No. 5679657, to achieve the required viscosity and penetrating properties required for cosmetic use.

Iminoglycinuria the compounds of formula I have an unusually low molecular weight and stable without obtaining derived for superior penetration through the skin to reduce wrinkles on the skin surface and also have a excellent softening and moisturizing effects.

A detailed description of the invention

Waste eggshell received ompromising pererabotki eggs, typically washed with solvents and process for eliminating unpleasant odors prior to disposal. The calcium carbonate of the shell can be used only after the most common methods of separation and purification, which make this process commercially uneconomical. There is no particular need for chopping eggshell within a narrow range, since the method according to this invention do not need to separate the membrane from the eggshell, as for complex method and equipment described MacNeil (U.S. patent No. 6176376), to obtain pure calcium carbonate.

Identify ways in which the selective extraction of valuable organic compounds, especially aminophenolic compounds of the formula I, from the crushed egg shell without costly separation of the organic and inorganic components.

Crushed eggshells can be treated with warm water or warm 5% solution of ethanol and filtered to remove adhering organic waste from the surface of the shell. The ratio of organic matter and calcium carbonate may be from 1% to 15% wt./weight. Large ratios of organic matter would serve as a sign lepromatous egg mass in crushed eggshells, which can lead to the presence of harmful protein and nucleotide products in the extract aminoglycan. Mark is, however, unlike other sources aminoglycan, such as animal tissue, fermentation broths, as is known from the prior art, the use of waste egg shell, as shown here, is unique due to the lack of significant antigenic protein and nucleotide components extracted in an environment that provides easier ways of extracting purified aminoglycan the compounds of formula I. an Egg shell can optionally be pre-treated with UV light to kill germs that may be present even after cleaning liquid.

The next step involves exposure of the above-mentioned mass-shell eggs highly selective extraction of the hydrocarbon component in the form of its water-soluble salts. The methods include the suspension of the mass of the egg shells of 1:2-1:10 aqueous salt solution containing 5%-40% by weight of salts of citrate, glutamate, acetate, pyrrolidinecarbonyl, tartrate, glycinate, sulfate, sulfite, nitrate, carbonate and oxalate sodium, potassium, calcium or magnesium or a combination of the above salt solutions, if needed. More specifically, the preferred monovalent organic acid salts. The suspension is incubated for 1-24 hours, more preferably within 6-12 hours, with occasional vigorous shaking at tempera is Urach in the range from 10C to 35C. Then the suspension is filtered or centrifuged to collect the aqueous solution containing the salt aminophenolic compounds of formula I. the Mass of eggs, separated, thus, exhibits a much lower binding to membranes from the egg shells, and as a result it can be much easier to handle by methods known in the art, to separate the egg shell containing pure calcium carbonate from an organic residue.

The next step involves the deposition of the gel aminoglycan the compounds of formula I in the form of a corresponding salt from the aqueous solution. The method includes reducing the polarity of the aqueous solution and the solubility aminoglycan the compounds of formula I by gradual adding of any miscible with water, an organic solvent, such as alcohols, acetone, dimethylformamide, N-methylpyrrolidinone or 1,4-dioxane. The organic solvent is added in portions with mild stirring and cooling to maintain the reaction temperature from 20C to 25C to obtain a white gel suspended in the aqueous layer. The solution is left for 2-24 hours until the gel is completely formed. Then gel filtered or centrifuged to obtain a semi-dry extract aminoglycan the compounds of formula I. it is Important not to allow the extract to dry completely, as it is necessary is definitely the aqueous phase for stabilization, which do next.

The last phase involves the stabilization of low molecular weight aminoglycan the compounds of formula I of distribution of lipophilic molecules in the environment to prevent the stitching, which is typical for deacetylating and low-molecular aminoglycan, as described in prior art (U.S. patent No. 5679657). This step of the method involves the gradual addition of the two oils. First oil is more hydrophobic in nature and can be selected from oils, which typically contain nuts. Especially as the first oil more preferably almond oil or jojoba oil. The second oil is more hydrophilic in nature and can be selected from oils, which are usually isolated from herbs and vegetative parts of plants. Especially as the second oil more preferably sage oil, rosemary oil or lavender oil. The number of all added oils may range from 5% to 50% of the weight of extracted aminoglycan, whereas the ratio of the two oils may be from 0.1:1 to 1:0,1.

Molecular weight aminoglycan the compounds of formula I, selected thus, it is difficult to measure directly, and, thus, rely on the measurement of the characteristic viscosity to determine the molecular weight (Laurent and others, Biochimica et Biophysica Acta, Vol.42, pg. 476 (1960)). Was detected by the network, what is the characteristic viscosity of various solutions containing aminoglycoside compound of formula I ranges from 4 cm3/g to 7 cm3/g, and when put on the schedule, based on the standard solutions of salts of hyaluronic acid (mol. weight approximately 1.2 MD), it is only possible natural ultra-low molecular weight aminoglycan the compounds of formula I in the range from 15,000 daltons to 28000 daltons. Ultramicroelectrode aminoglycoside compound of formula I from a natural source was previously described in the prior art (for example, concluded Balazs and others in U.S. patent No. 4582865).

The results of the experiment

Example 1

In an open hole glass container with a screw cap was added 500 g of pre-treated waste egg shell with approximately 10% organic content. Added 750 ml of 5% aqueous solution of sodium citrate, the container was sealed and placed on a shaker for 24 hours at medium speeds. After 24 hours, the entire mixture was transferred into a funnel with filter and solid waste egg shell was separated from the aqueous slurry. The solids were washed 1250 ml of 5% aqueous solution of sodium citrate, the combined aqueous layers once washed 250 ml of methylene chloride to remove possible melkovodnogo substances and then the aqueous layer was lane who was carrying a 2-litre bowl. The Cup was placed in a bath of cold water and slowly add absolute methanol under slow stirring. After he finished to add approximately 200 ml of methanol, began to form a cloudy white precipitate, and the stirring stopped. Slowly added is equivalent to the additional amount of methanol, and the Cup was left for 12 hours to ensure that the gel formation was completed. The whole mass moved into the funnel with the filter and filtered to obtain a creamy gel aminoglycan the compounds of formula I. the Precipitate was dried up until the moisture content was 5-7%. The final weight of the gel aminoglycan the compounds of formula I was 42,

Example 2

Gel material containing aminoglycoside compound of formula I from example 1 was mixed with 4 g of jojoba oil at 15C-20C and vigorously stirred for 20 minutes. The resulting gel was heated to 25C and gently stirred for 1 hour. To this mass was added 1 g of oil of sage, and the resulting gel is then gently stirred for 10 minutes. Then the gel was slowly cooled to 10C for 4 hours to obtain aminoglycan the compounds of formula I, which is stable in the absence of circulating air at room temperature for at least 3 months.

Example 3

The above-mentioned example 1 was repeated with the 10% aqueous solution of potassium tartrate to obtain 46 g of gel aminophenolic compounds of formula I.

Example 4

The above-mentioned example 1 was repeated with 20% sodium acetate solution to obtain 43 g of gel aminophenolic compounds of formula I.

Example 5

The above-mentioned example 1 was repeated, except that instead of methanol was used ethanol for the complete formation of the gel to obtain 47 g of gel aminoglycan the compounds of formula I.

Example 6

The above-mentioned example 1 was repeated, except that instead of methanol was used acetone to complete formation of the gel to obtain 41 g of gel aminoglycan the compounds of formula I.

Example 7

The above-mentioned example 1 was repeated with 10% sodium carbonate solution to obtain 24 g of gel aminoglycan the compounds of formula I.

Example 8

The above-mentioned example 1 was repeated with 25% solution of calcium carbonate to obtain 14 g of gel aminoglycan the compounds of formula I.

Example 9

10 g of the above-mentioned stabilized gel obtained according to the method shown in example 2, was added to 50 ml of distilled water containing 3 ml of glycerin, and mixed until a homogeneous suspension. To this suspension was added to the melt, consisting of 10 g emulsifying wax, 10 g of paraffin wax, 4 g white beeswax and 13 g of the mixture used in cosmetics vegetable oils, such as almond oil, lavender, sandalwood and walnut, and the mixture is vigorously stirred is to obtain a homogeneous cream with excellent physical characteristics and anti-wrinkle properties.

Taking into consideration the above-mentioned dedicated and stable gels aminoglycan the compounds of formula I, have completed the following analytical and practical research.

The absence of chondroitin sulfate

In the prior art it is known that all commercial sources aminoglycan usually closely linked to other components of the tissue, such as chondroitin sulfate (Arkins and Sheehan, Structure of Hyaluronic Acid, Nature New Biol, 235, 253, 1972, and Bettelheim and Philpott, Electron Microscopic Studies of Hyaluronic Acid. - Protein Gels, Biochim Biophys Acta, 34, 124, 1959). Gel extract, selected by means described above, contains less than 2% chondroitin sulphate, probably due to the low Association possible with a very small size of the allocated aminoglycan the compounds of formula I.

The absence of proteins

Because proteins are potentially antigenic, for cosmetic formulations, it is important to identify any gel aminoglycan, not containing mainly protein. Gel extract from example 1 was subjected to a highly sensitive colorimetric analysis to determine the presence of proteins, as described by Lowry and others (J. Biol. Chem., 193, 265-275, 1951). The result indicated the presence of only less than 0.1% protein by weight.

The absence of any significant concentrations of protein is a distinct difference from other compounds glycosaminoglycan isolated from natural the sources, such as a rooster's comb and fermentation broths. Reported (Kludas, U.S. patent No. 5055298)that the data aminoglycan usually covalently bound to proteins for the formation of proteoglycans. Clinically relevant eliminating all those proteins that are not components of human skin, proved to be difficult to perform. The presence of these proteins in various other extracts aminoglycan identified as the cause of the characteristic inflammatory reactions in the skin surface, which complicates their use in cosmetic formulations.

The absence of nucleotides

Ultraviolet spectroscopy was used to show the absence of potentially antigenic nucleotides of DNA and RNA in a dedicated aminoglycan the compound of formula I. Prepared 1% solution of the extract aminoglycan example 1 in a 10% solution of sodium chloride. This solution was subjected to UV spectroscopy at 257 nm for measuring the level of nucleotides in solution. The absence of any absorption at this wavelength was taken as a measure of the absence of nucleotides in the extract aminoglycan example 1.

Viscosity

A small sample of the gel was dried by sublimation to obtain white solids with a filamentary structure, which was slowly dissolved in water. A solution of 1 mg of powder was placed in 1000 ml phosphate buffer pH 7. The viscosity was measured in in the ScopeMeter Ostwald at 25C. The measured relative viscosity of the solution ranged from 0.76 to 0.80. Compared with aminoglycoside known high molecular weight, the viscosity measurement gives the molecular weight for iminoglycinuria the compounds of formula I from 15,000 daltons to 28000 Dalton.

The presence of glucosamine

The presence of glucosamine in aminoglycan the compound of the formula I are investigated by the method of Elson and Morgan (Biochem J, Vol.27 (1933), p.1894) on the material, hydrolyzed for 6 hours 5N hydrochloric acid at 100C and evaporated to dryness. The content of glucosamine aminoglycan the compounds of formula I ranged from 38% to 41%, which corresponded to the expected calculated value.

The presence of uronic acids

The presence of uronic acids in connection aminoglycan formula I was determined by hydrolysis with hyaluronidase. Extracted aminoglycoside compound of formula I washed with distilled water and hydrolysable Streptomyces hyaluronidase (1 mg enzyme/g aminoglycan) in 10 ml of 10 mm CaCl2/50 mm-Tris/HCl buffer, pH 7.6 for 48 hours at 37C. Inhibitors proteinase, namely phenylmethanesulfonyl (2 mm) and N-ethylmaleimide (10 mm)was added to the samples for inhibition of non-specific proteolysis. The hydrolysis was stopped by the addition of urea to a final concentration of 6 M. the Hydrolysate was centrifuged at 4000g, supernatant (hydrolyzed hyaluronidase) were removed and contrasted standard uronic acid by analysis of HPLC (high performance liquid chromatography).

The ability to niteobraznaja

In the prior art it is well documented that the higher the ability to niteobraznaja, the more moisturizing effect aminoglycan. Have used many derivatives aminoglycan medium and high molecular weight, e.g. acetylation and copolymerization (U.S. patent No. 5679657) to increase the inherent degree of niteobraznaja aminoglycan isolated from animal and bacterial sources. Observed that ultramicroelectrode aminoglycoside compound of formula I, shown here, shows an exceptionally high ability to niteobraznaja and may explain part of the observed high effects against wrinkles. In a humid chamber at 25C and a relative humidity of 50% glass rod 1 cm was immersed in 1% aqueous solution of the extract aminoglycan example 1 and examined the length of the filament obtained by immersion of the bowl at the speed of 10 cm/min. Length of thread aminoglycan the compounds of formula I according to this invention ranged from 2.8 cm to 3.5 cm, which is much longer than 0.8 cm to 1.3 cm, which was observed in commercially available sodium hyaluronate, and even better, than the length of the observed derivatives aminoglycan.

Wrinkle properties

Researched-wrinkle properties of the cream obtained in a way that op is San in example 9, applying the image processing system for 3D measurement of the depth of surface lines and wrinkles. Used the method described by S. Jaspers, and others, ("Microtopometry Measurement of Human Skin in vivo by a new Digital Projection Optical System", Preprints 5th Congress of the International Society for Skin Imaging, Wien, 1997)to show the reduction of wrinkle depth by 25% - 38% after 4 weeks of daily use.

1. The method of selection of low-molecular aminophenolic compounds of the formula I from waste egg shell,
,
where M may be one or more of Na, CA, K, Mg, and n is an integer from 20 to 40;
moreover, the method includes the steps are:
(a) pre-prepare the waste egg shell to extract embryonic molecular aminoglycan the compounds of formula I using a polar organic solvent in water and egg shells are sent to extraction;
(b) extracted low-molecular aminoglycoside compound of formula I in the form of its water-soluble salts, for this egg shell stage (a) vigorously shaken with aqueous polar salt solution at 10-35C for 6-12 h, and then filtered or centrifuged to collect the aqueous layer containing dissolved aminoglycoside compound of formula I;
(c) produce a purified low molecular weight aminoglycoside compound of formula I through the formation of the deposits of gel from an aqueous mixture of salts using a polar organic solvent, to do this, in the solution of step (b) sequentially incrementally add miscible with water, an organic solvent under mild stirring and cooling to maintain the temperature from 20 to 25C and the formed gel is left for 2-24 hours to complete the precipitation, and then filtered or centrifuged to highlight semi aminoglycan the compounds of formula I;
(d) stabilize the selected aminoglycoside compound of formula I of step (C) the gradual introduction of organic oils in semi-dry gel formation aminoglycan the compounds of formula I.

2. The method according to claim 1, where the polar organic solvent used in step (a), selected from the group including alcohol, acetone, methyl ethyl ketone or 1,4-dioxane.

3. The method according to claim 1, where the specified polar water salt solution used in step (b)is chosen from the group comprising sodium, potassium, calcium or magnesium citrate, glutamate, acetate, pyrrolidinecarbonyl, tartrate, glycinate, sulfate, sulfite, nitrate, carbonate or oxalate salt.

4. The method according to claim 1, where the polar organic solvent used in step (C)is a lower alcohol selected from methanol, ethanol, propanol or butanol, or an organic ester selected from diethyl ether, tetrahydrofuran, matilla or Atilla.

5. Spasibo to claim 1, where organic oils used in step (d)are oils derived from plant sources.

6. The method according to claim 5, where the aforementioned organic oil chosen from oils of jojoba, almond, sage, rosemary, lavender, sandalwood or red.

7. A method of manufacturing a composition having anti-wrinkle properties, including aminoglycoside compound of formula I according to claim 1, whereby the method includes the steps are:
(a) pre-prepare the waste egg shell to extract embryonic molecular aminoglycan the compounds of formula I using a polar organic solvent in water and egg shells are sent to extraction;
(b) extracted low-molecular aminoglycoside compound of formula I in the form of its water-soluble salts, for this egg shell stage (a) vigorously shaken with aqueous polar salt solution at 10-35C for 6-12 h, and then filtered or centrifuged to collect the aqueous layer containing dissolved aminoglycoside compound of formula I;
(c) produce a purified low molecular weight aminoglycoside compound of the formula I by means of forming a gel from an aqueous mixture of salts using a polar organic solvent in the solution of step (b) sequentially incrementally add miscible with water, organic is the cue solvent under mild stirring and cooling to maintain the temperature from 20 to 25C, and the formed gel is left for 2-24 hours to complete the precipitation, and then filtered or centrifuged to highlight semi aminoglycan the compounds of formula I;
(d) stabilize the selected aminoglycoside compound of formula I of step (C) the gradual introduction of organic oils in semi-dry gel formation aminoglycan the compounds of formula I;
(e) add at least one pharmaceutically acceptable excipient to a stable aminoglycan the compounds of formula I obtained in step (d)to form a composition having anti-wrinkle properties.

8. Stable aminoglycoside compound of formula I having a molecular weight in the range from 15,000 to 28000 Yes, obtained by the method according to claim 1.



 

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3 ex

FIELD: chemistry.

SUBSTANCE: starting high-molecular chitosan is dissolved in acid solution. The chitosan dissolved in the acid is then precipitated by adding alkali solution. The re-precipitated high-molecular chitosan is washed from the formed salt and excess alkali using a coarse-porous filter. The re-precipitated chitosan is dissolved in acid solution until achieving pH 5.5. An enzyme preparation is then added and hydrolysis is carried out. The reaction is stopped after formation of low-molecular chitosan.

EFFECT: method is characterised by avoiding the need to remove salts from the enzymatic mixture and the end product, as well as low level of loss of material.

2 dwg, 7 tbl, 6 ex

FIELD: chemistry.

SUBSTANCE: reaction is carried out in a two-phase system: aqueous potassium hydroxide solution - toluene at room temperature for 3-11 hours with further extraction of the product with acetone and extraction of the product from the organic layer. Extraction of the products from the organic layer and purification is carried out by deposition into ethyl alcohol.

EFFECT: invention enables to obtain novel propargyl esters of arabinogalactan by realising the method under mild conditions.

3 cl, 5 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to novel complexes of Platelet-Derived Growth Factor (PDGF), associated with amphiphilic polymers, ensuring physical and chemical stability of protein in respect of decomposition at physiological pH in vitro for application in pharmaceutics. Complex amphiphilic polymer-PDGE possesses chemical and physical stability, is water-soluble, polymer being selected from polysaccharides. Polymer consists of hydrophilic polymer skeleton, functionalised by hydrophobic substituents and hydrophilic groups. Invention also relates to method of obtaining complex amphiphilic polymer-PDGE, which is obtained in water medium in absence of organic solvent, able to denature protein, and to application of said complex amphiphilic polymer-PDGE for obtaining therapeutic composition with healing action, intended for treatment of ulcers by topic application.

EFFECT: improvement of physical and chemical stability of therapeutic protein in vitro and in vivo.

17 cl, 3 ex

FIELD: chemistry.

SUBSTANCE: oyster plant is washed, crushed and then extracted with acid water in phase ratio of 1:1.2, pH 5-6.5, temperature 55-65C, while periodically releasing pressure in the extraction mixture until the extraction agent boils. The extraction mixture undergoes preliminary processing in a microwave field until inactivation of native enzymes. The separated extract is concentrated through reverse osmosis to 15-20% content of dry substances, undergoes photosterilisation and packed in aseptic conditions.

EFFECT: invention enables reduction of chromaticity of the end product at relatively high conversion of inulin to the end product, as well as reduction of energy consumption and ensures shelf life of the end product of not less than 1 year without considerable change of its consumer and technological properties.

FIELD: chemistry.

SUBSTANCE: invention relates to a technique for preparing an inulin-containing solution for food or medical purposes. Dandelion root is washed, crushed and extracted with acid water in phase ratio of 1:1.2, pH 5-6.5 and periodic pressure release in the extraction mixture before the extraction agent boils. The extracted mixture undergoes preliminary electrocontact heating to temperature 55-65C. The separated extract is concentrated through reverse osmosis to 15-20% content of dry substances, subjected to photosterilisation and packed under aseptic conditions.

EFFECT: invention reduces chromaticity of the end product with relatively high conversion of inulin to the end product, reduces energy consumption and ensures shelf life of the end product of not less than 1 year without considerable change of its consumer and technological properties.

FIELD: chemistry.

SUBSTANCE: invention relates to a technique for preparing an inulin-containing solution for food or medical purposes. Dandelion root is washed, crushed and extracted with acid water in phase ratio of 1:1.2, pH 5-6.5, temperature 55-65C and periodic pressure release in the extraction mixture before the extraction agent boils. The extraction mixture undergoes pre-treatment in a microwave field until inactivation of native enzymes. The separated extract is concentrated through reverse osmosis to 15-20% content of dry substances, undergoes photosterilisation and then packed in aseptic conditions.

EFFECT: invention reduces chromaticity of the end product with relatively high conversion of inulin to the end product, reduces energy consumption and ensures shelf life of the end product of not less than 1 year without considerable change of its consumer and technological properties.

FIELD: chemistry.

SUBSTANCE: invention relates to a technique for preparing an inulin-containing solution for food or medical purposes. Scorzonera is washed, crushed and extracted with acid water with separation of the extract in phase ratio of 1:1, pH 5-6.5, temperature 55-65C for 30-40 minutes and periodic pressure release in the extraction mixture before the extraction agent boils. The extraction mixture undergoes pre-treatment in a microwave field until inactivation of native enzymes. The separated extract is concentrated through reverse osmosis to 15-20% content of dry substances, undergoes photosterilisation and then packed in aseptic conditions.

EFFECT: invention reduces chromaticity of the end product with relatively high conversion of inulin to the end product, reduces energy consumption and ensures shelf life of the end product of not less than 1 year without considerable change of its consumer and technological properties.

FIELD: medicine.

SUBSTANCE: method provides washing and grinding of oyster plant, acid water filling, electrical contact heating, extraction at the preset process parameters, return osmosis extract concentration, photosterilisation and packaging in the aseptic conditions.

EFFECT: method allows a relatively high yield of inulin in an end product with reduced chromaticity of the latter, reduced power inputs and provided shelf life of the end product for at least 1 year without considerable change of its consumer and technological properties.

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to method of obtaining composition, which has antioxidant activity, containing as main component proanthocyanidin oligomer, which has degree of polymerisation from 2 to 4, which includes stage of heating of proanthocyanidin polymer - containing vegetable materials, or their extract with green tea or its extract or epigallocatechinegallate in water solution of acid and stage of concentration of reaction solution, containing proanthocyanidin oligomer.

EFFECT: claimed method allows to efficiently and easily obtain from vegetable raw material with high output composition of proanthocyanidin oligomer, to whose end position bound is fluoroglycine ring structure and whose molecular weight is reduced to such value, that oligomer can be absorbed in live organism.

8 cl, 17 dwg, 11 ex, 2 ex

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