Method of determining quantity of fibrin-monomers in blood
SUBSTANCE: quantity of fibrin-monomers, dissolved in 0.5 N sodium hydroxide, is determined spectrophotometrically with application of ethanol test. Claimed method of quantitative determination of fibrin-monomers in blood makes it possible to reveal pathological process in organism with 95% reliability.
EFFECT: increase of determination accuracy.
2 tbl, 4 ex
The invention relates to medicine, namely to biochemical method for determining the concentration of components of the coagulation and products of their transformation in biological fluids.
In many diseases and pathological conditions (hemorrhagic and ischemic strokes, atherosclerosis, myocardial infarction, surgical trauma, cesarean section, and so on) there is a need for quantitative determination of hybridmonolith and degradation products of fibrin in plasma, however, to date only apply qualitative methods of determining the presence of hybridmonolith in plasma.
There is a method of identifying hybridmonolith in plasma for the formation of gelatinous masses under the influence of 50% ethanol [Lychev VG Diagnosis and treatment of disseminated intravascular coagulation. - M.: Medicine, 1993. - 160 S.]. Releasetest plasma 10 min after addition of 50% ethanol is considered as a sign of the presence of hybridmonolith. If form coarse particles, then the result is evaluated as slabopolozhitelnym or negative. The method does not allow to determine the concentration of hybridmonolith.
Angomont et al. proposed method for the determination of hybridmonolith and degradation products of fibrin on education in the plasma grains (procoagulant) fibrin after you add the possible solution phenanthroline [Moment A.N., Lakomov VA, Barkagan SS Clinical and laboratory diagnosis. 1996. No. 4. - P.17-20]. The main disadvantages of the method are its subjectivity and the presence of false positive results that arise, in particular, as a result of insufficient mixing of blood and citrate or storage of plasma for more than 1 hour, which leads to inactivation of coagulation factors.
The present invention is to develop an objective method for determining the number of hybridmonolith in plasma.
The technical result is simple, does not require high material costs, based on spectrophotometric determination of hybridmonolith.
The technical result is achieved in that the precipitate plasma homogenized in 0.5 N sodium hydroxide spend spectrophotometric analysis of suspension and the number of hybridmonolith determined by phrogram.
The positive effect of the proposed method achieve due to the quantitative determination of hybridmonolith spectrophotometrically. Comparative characteristics of the proposed method and the method of the prototype are presented in table 1.
The proposed method is as follows.
From the cubital vein blood sample in a plastic test tube containing 3.8% solution of sodium citrate 3-substituted (sodium citrate). The ratio of the volume of the RH blood and citrate - 9:1 (4.5 ml blood and 0.5 ml of sodium citrate). The contents centrifuged at 4000 rpm for 10 min (2000g). The result is a platelet-poor plasma, then 0.4 ml of blood plasma is added 0.15 ml of 50% ethanol and centrifugum at 3500 rpm for 15 minutes Received centrifugal (sludge) wash of 0.14 M solution of sodium chloride and centrifugum twice at 3.500 rpm for 10 min. the precipitate homogenizers in 7 ml of 0.5 n solution of sodium hydroxide within 1-2 min and placed in a quartz cuvette for spectrophotometry at 280 nm transmittance and phrogram determine the number of hybridmonolith. Fluctuations in the extinction from 0.1 to 0.8 indicate concentrations hybridmonolith in plasma.
Examples of the practical use of the proposed method
In the neurological Department Bureau No. 2 Tyumen entered patient Shcherbakova A.I., 68 years old with the diagnosis of Ischemic stroke" (moderate severity).
Patient blood samples were taken from the cubital vein into a syringe with a stabilizing solution (3,8% aq sodium citrate) in the ratio 1:9. A sample of 5 ml was placed in a centrifuge tube and centrifuged with acceleration 2000g for 10 min, then 0.4 ml of plasma was transferred to another tube and was added 0.15 ml of 50% ethanol. Included stopwatch and after 10 min was noted by the appearance of grains of procoagulant, then centrifuge holds Aravali at 3500 rpm 15 minutes Received centrifugal (sludge) were washed of 0.14 M sodium chloride solution and twice centrifuged at 3500 rpm (acceleration 1500g) for 10 min Decanted removed. The precipitate homogenized in 7 ml of 0.5 n solution of sodium hydroxide within 1-2 min and were placed in a quartz cuvette for spectrophotometry at 280 nm transmittance and phrogram determine the number of hybridmonolith. Concentration of hybridmonolith in plasma were judged by the magnitude of the refraction of light. Registered transmittance - 0,41.
Sick men E.N., 46 years was delivered to the Department of reanimacii design Bureau No. 2 Tyumen SMP machine in an emergency. Clinically diagnosed with "Ischemic stroke" (high severity).
From the cubital vein blood samples were taken in a plastic test tube containing 3.8% solution of sodium citrate 3-substituted (sodium citrate). The ratio of the volume of blood and sodium citrate to 9:1 (4.5 ml blood and 0.5 ml of sodium citrate). The contents were centrifuged at 4000 rpm for 10 min (2000g). The result made a club platelet-poor plasma, then 0.4 ml of plasma was added 0.15 ml of 50% ethanol and centrifuged at 3500 rpm for 15 minutes Received centrifugal (sludge) were washed of 0.14 M sodium chloride solution, and then centrifuged twice at 3.500 rpm for 10 min. the precipitate homogenized in 7 ml 05 N. solution of sodium hydroxide within 1-2 min and were placed in a quartz cuvette for spectrophotometry at 280 nm transmittance and phrogram determined the number of hybridmonolith. The transmittance is 0.76, indicating that the concentration of hybridmonolith in plasma.
To quantify the content of hybridmonolith in the blood plasma used phrogram, which is in units of activity reflects Concentratio of hybridmonolith, corresponding to 6.0 EA.
In the neurological Department Bureau No. 2 Tyumen entered patient Lagutina L.A., 48 years with a diagnosis of Ischemic stroke" (mild severity).
From the cubital vein blood samples were taken in a plastic test tube containing 3.8% solution of sodium citrate 3-substituted (sodium citrate). The ratio of the volume of blood and sodium citrate to 9:1 (4.5 ml blood and 0.5 ml of sodium citrate). The contents were centrifuged at 4000 rpm for 10 min (2000g). As a result he received platelet-poor plasma. Then 0.4 ml of plasma was added 0.15 ml of 50% ethanol and centrifuged at 3500 rpm for 15 minutes Received centrifugal (sludge) were washed of 0.14 M sodium chloride solution and centrifuged twice at 3500 rpm for 10 min. the precipitate homogenized in 7 ml of 0.5 n solution of sodium hydroxide within 1-2 minutes Received) suspension was placed in a quartz Kyu the etu for spectrophotometry. Used a wavelength of 280 nm.
To quantify the content of hybridmonolith in the blood plasma used phrogram, which reflects the light transmission in units of activity. Light transmission rate amounted to 0.27, and according to phrogram - 2,2 EA.
Patient eagles E.A., 34 years, was delivered to the Department of reanimacii design Bureau No. 2 Tyumen SMP machine in an emergency. Clinical diagnosis of Ischemic stroke high severity".
Patient blood samples were taken from the cubital vein into a syringe with a stabilizing solution (3,8% aq sodium citrate) in the ratio 1:9. A sample of 5 ml was placed in a centrifuge tube and centrifuged with acceleration 2000g for 10 min, then 0.4 ml of plasma was transferred to another tube and was added 0.15 ml of 50% ethanol. Included stopwatch and after 10 min note the appearance of grains of procoagulant, then centrifuged at 3500 rpm for 15 minutes Received centrifugal (sludge) were washed of 0.14 M sodium chloride solution and twice centrifuged at 3500 rpm (acceleration 1500g) for 10 min Decanted removed. The precipitate homogenized in 7 ml of 0.5 n solution of sodium hydroxide within 1-2 min and were placed in a quartz cuvette for spectrophotometry at 280 nm transmittance and phrogram determined the number of hybridmonolith. On the concentration of the emission of hybridmonolith in plasma judged also by the magnitude of the refraction of light. Registered transmittance - 0,79.
The examples illustrate the applicability of this method for the quantitative determination of hybridmonolith in plasma on the basis of spectrophotometric analysis and results phrogram. Altogether there were 20 studies with blood plasma from patients undergoing treatment in the regional clinical hospital №2 Tyumen (table 2). The positive effect of applying the proposed method in comparison with the prototype presented in table 2. The proposed method allows to detect the pathological process in the body with a certainty of 95%.
The method of quantitative determination of hybridmonolith in the blood
|Comparative characteristics determine hybridmonolith blood on the proposed method and prototype|
|Signs||The proposed method||Prototype method|
|1. Ispolzovanie reagents for research||50% solution of ethanol||solution phenanthroline|
|2. Processing plasma for research||Repeated centrifugation||Not done|
|3. Resolution method||Quantitative determination of hybridmonolith||Qualitative determination of hybridmonolith|
|4. Objectivity of research||Spectrophotometric determination of hybridmonolith||Visual definition of research results|
|5. Determining the severity of the pathological process in order to assign specific treatment||The possibility of using the method to assign specific treatment||Specific treatment is not provided|
The method of quantitative determination of hybridmonolith in the blood
|Fluctuations in the readings of the optical density in patients with ischemic stroke|
|The sequence number||The indicators light transmission||EA per ml|
Method of determining hybridmonolith in the blood, including the use of an ethanol test, characterized in that after treatment of plasma with ethanol, centrifugation and washing the precipitate with sodium chloride re-centrifugation, the precipitate homogenized with 0.5 N sodium hydroxide within 1-2 minutes, and the result take into account on the basis of spectrophotometric analysis at 280 nm transmittance and the number of hybridmonolith determined by ferrogram.
SUBSTANCE: thrombosis monitor comprises: a thrombosis chamber, at least in a part of which there is a thrombogenic material; an inlet tube connected to the thrombosis chamber through which blood flows into the thrombosis chamber; a blood supply container connected to the inlet tube; a feed pump for the container; a pressure sensor for measuring pressure applied to the container. A method of thrombosis monitoring consists in the fact that after introduction of an anticoagulant, blood is supplied from the container to the thrombosis chamber by pressing on a fluid placed on a blood layer and having density less than that of the blood layer; it is combined with anticoagulation blood processing or blood coagulation stimulation, and measurement of pressure applied to the container; the thrombogenic material is placed at least in a part of the thrombosis chamber.
EFFECT: group of inventions provides overall assessment of blood coagulation and platelet-cell thrombosis in a medium equivalent to blood flow for evaluation of efficacy of an antithrombotic drug.
11 cl, 15 dwg, 23 ex
SUBSTANCE: for determination of functional state of hemostasis system record of blood coagulation process is performed, current amplitude of blood resistance in first time moment is registered and second resistance of blood at multiple time moment from initial time value is measured. Two resistances and time moments are used to determine maximum blood resistance and time constant, by which blood resistance at the beginning and end of coagulation process is calculated. Obtained parameters are used to determine indices of beginning and end of blood coagulation process. Obtained indices are compared with of the same name indices of blood coagulation process in norm and in case of differently directed deviations disturbances of functional state of hemostasis system are diagnosed.
EFFECT: invention makes it possible to increase measurement accuracy and reduce examination time.
1 tbl, 4 dwg
SUBSTANCE: method is based on a method of observing turbidimetric fibrin clot formation with optical transmission of an incubation medium recorded by ultraviolet radiation band 230 to 320 nm by means of UV-spectrophotometre as a fibrin-polymer detector.
EFFECT: invention enables higher accuracy and sensitivity of the method.
4 ex, 4 dwg
SUBSTANCE: for thrombin production measurement, a layer of said sample contacts with a fluorogenic substratum of thrombin where the thickness of said layer is 0.05 to 5 mm, while the surface area is 10 to 500 mm2. Further, the thrombin production environment in said sample is provided. It is followed by measuring the fluorescence emitted from the layer surface by a fluorescent group released by the fluorescent substratum as a result of an enzymatic action of produced thrombin on said fluorogenic substratum. Besides, the invention ensures a kit for measuring the thrombin activity in the sample.
EFFECT: higher measuring accuracy.
29 cl, 12 dwg, 5 ex
SUBSTANCE: blood is examined. A hematocrit level (H), erythrocyte count (E), thrombocyte count (T) are determined. Said parametres are evaluated. In the event if they keep within the determined limits for the patients with acute coronary syndrome (ACS), then adenosine phosphate induced (ADP-induced) clotting time test samples are prepared. Citrated blood sample 0.4 ml is prepared of whole blood and divided on two samples 0.2 ml. Each of these samples is introduced in a measuring cell, recalcified at temperature 37°C for 2 minutes. Then a magnetic ball mixer is placed in each cell. The measurement is activated, and in three seconds the ADP solution 0.1 ml is introduced. After a clotting reaction, a process time duration is recorded separately for each sample. An arithmetical mean of the derived values is calculated (A). The derived values of each of said parameters are scored. Total score Σ=A+H+E+P shows the risk of recurrent thrombotic events. If Σ=4 points, the low risk is observed; the value Σ=5-6 points shows the medium risk, while Σ=7-10 points - the high risk.
EFFECT: method provides more objective risk evaluation of recurrent thrombotic events in the patients with ACS with its simplicity and low cost.
1 ex, 1 tbl
SUBSTANCE: blood sample is placed in capillary, in whose walls installed are electrodes connected to frequency generator and registering unit, blood electric conductivity is measured at the moment of passing through it of alternating current with frequency 200 Hz, electric coagulogram is registered and used to determine chronometric and amplitude characteristics: A - amplitude of functional curve decline, mV; N - time of functional curve decline to minimal value in minutes. If value of A/T index decreases or increases with respect to normal, conclusion about hemostatic disorders is made. If value of A/T index equals 3-5 - hemostasis state is evaluated as normal, if A/T value is lower than 3, hypocoagulation is determined, and if A/T value is higher than 5 - hypercoagulation.
EFFECT: application of the method makes it possible to obtain data about hemostasis system state in real time mode, without injuring form blood elements in investigated microvolumes of blood, thus making it possible to increase accuracy, self-descriptiveness and efficiency of hemostasis state evaluation and to carry out correction of performed therapy without delay.
4 dwg, 3 ex
SUBSTANCE: in patients with IHD before therapy with acetylsalisylic acid (ASA) ADP-induced and ASA-dependent platelet aggregation are examined and by their difference value of coefficient of aggregation inhibition (CAI) is calculated. CAI value <24% testifies to resistance to ASA, if CAI ≥24% - about sensitivity to ASA.
EFFECT: method ensures high prediction accuracy and makes it possible to prevent development of undesirable coronary events in IHD patients.
1 tbl, 4 ex, 4 dwg
SUBSTANCE: invention refers to medicine, namely to haematology, and orthopaedics. The intravascular thrombocyte activity correction in children with scoliosis requires the prescription for at least 6 months of a therapeutic complex which involves daily therapeutic physical training, two courses of massage and daily swimming for at least 20 minutes a day.
EFFECT: method allows to normalise intravascular thrombocyte activity in children aged 8-12 with scoliosis, to improve tissue microcirculation considerably, to optimise growth and development of skeleton and internals.
SUBSTANCE: invention belongs to medicine, notably to haematology and orthopaedics. For thromboplastin synthesis correction in children 8-12 years old with scoliosis is prescribed complex of treatment, including daily remedial gymnastics, two courses of massage and daily swimming for at least 20 minutes a day, during 6 months.
EFFECT: method enables thromboplastin synthesis normalisation in children with scoliosis, significantly sanitate children with scoliosis due to improvement of tissues' microcirculation.
SUBSTANCE: invention refers to medicine, namely to aggressive medical therapy, resuscitation science, critical care medicine, laboratory diagnostics and can be used by resuscitators, intensivists, laboratory doctors for well-timed diagnosis and consequently, for individualised aggressive medical therapy of acute disseminated intravascular coagulation. The integrated assessment of links of haemostatic system and the clinical appraisal of organ dysfunction are applied in a measurer, and when observing structural hypercoagulation characterised by fibrinogen level increase, thrombocyte activity increase, growth of soluble fibrin complex (SFC) level, and also when observing chronometric hypercoagulation characterised by time tests, palette-derived factor 4 activity (P4) with manifested petechial haemorrhage and organ dysfunction, and coagulation cascade activation with underlying depression of antithrombin III and protein C, a hypercoagulation stage of acute DIC is diagnosed. Chronometric hypercoagulation by Activated partial thromboplastin time (APTT), INR, fibrinogen and P4 with manifested signs of structural hypocoagulation by thrombin time prolongation and D-dimer activity increase with underlying further intensification of anticoagulant system deficiency, progression of target organs dysfunction and mixed haemorrhage show a transitive stage of acute DIC. If observing said hypocoagulation changes and disturbed fibrinolytic activity with prevailing either decompensated organ and tissue dysfunction, or hemorrhagic syndrome up to system haemorrhages, or their combination with hemorrhagic syndrome characterised by polymorphism of clinical picture and localisation: petechial-haematoma haemorrhage at the stress-induced stomach ulcers, hematuria, a coagulopathy stage of acute DIC is diagnosed that is characterised either by depression of fibrinolysis, or preserved fibrinolytic activity, or by activation of secondary fibrinolysis, or by acute primary fibrinolysis.
EFFECT: method allows optimising classification of acute disseminated intravascular coagulation, improving diagnostic significance of the classification and simplifying a diagnostic prospecting which provide a basis to consider the staging of acute disseminated intravascular coagulation.
1 dwg, 4 ex
FIELD: medicine, laboratory diagnostics.
SUBSTANCE: the suggested studying should be carried out on the glass simultaneously with several inductors by applying minimal inter-taking antilogarithms concentrations of aggregation inductors which correspond at double combination of inductors: ADP 5.0 x 10-8 M, adrenaline 3.0 x 10-9, collagen - dissolving the main suspension 1:8, thrombin 0.075 U/ml; at triple combination of inductors: ADP 10-9 M, adrenaline 10-9, collagen - dissolving the main suspension 1:9, thrombin 0.060 U/ml. The development of aggregation means thrombocytic activation in patients with arterial hypertension at metabolic syndrome. The method enables to evaluate the changes of thrombocytic functional state with combination of inductors more probably present in area of vascular lesion by applying minimal necessary concentrations that develops real conditions at hemostatic initiation in human vessels.
EFFECT: higher efficiency of studying.
3 dwg, 3 ex, 2 tbl
SUBSTANCE: method involves checking consciousness, blood coagulation state, peripheral blood leukocytes number, K+ ions, bilirubin, fibrinogen, hemolysis and hemoglobinuria availability, prothrombin index and exotoxic shock development. Each value is calculated in points as follows. Lucidity is evaluated as -2 points; depression - +3 points; coma - +6 points; lack of changes in blood coagulation system - -2 points; coagulation availability without clinical injuries - +2 points; coagulopathy with clinical manifestation signs - +19 points; K+ ions concentration being less than 3.0 mmole/l - +3 points, from 3.1 to 3.5 mmole/l - -5 points, from 3.6 to 5.0 mmole/l - 0 points, greater than 5.0 points - +7 points, failure in determining K+ ions concentration - 0 points; hemolysis availability - +6 points, its lack - -3 points; hemoglobinuria availability - +8 points, its lack - -1 points; leukocytes number being less than 12.0x109/l - -2 points, from 12,1 to 18.0x109/l - 0 points, higher than 18.0x109/l - +8 points; hourly urine output being less than 30 ml/h - +6 points, greater than 30 ml/h - -2 points; bilirubin content being less than 31 mcmole/l - -2 points, from 30.1 to 50.0 mcmole/l - 0 points, greater than 50.0 mcmole/l - +2 points, failure in determining bilirubin content due to hemolysis being available -+6 points; prothrombin index being equal to or less than 60% - +3 points, greater than 60% - 0 points, failure in determining prothrombin index due to hemolysis being available - +12 points; fibrinogen concentration in blood plasma being less than 2.1 g/l - +4 points, from 2.1 to 4.0 g/l - -1 point, from 4.1 to 6.0 g/l - +1 point, failure in determining fibrinogen concentration due to erythrocyte hemolysis being available - +13 points; exotoxic shock development - +9 points, its lack - -1 point. The points are summed up. The value being greater than +13, admission for treatment in resuscitation department is indicated. The value being less than -13, admission for treatment in therapeutics department is indicated. The value being from -13 to +13, resuscitation expert consultation is advised.
EFFECT: high evaluation accuracy.
FIELD: medicine, laboratory diagnostics.
SUBSTANCE: one should evaluate the time for clotting of plasma under testing in phospholipid-dependent test, moreover, one should apply high- and low-sensitive thromboplastin reagents to lupus anticoagulant to calculate the ratio of indices of prothrombin time prolongation and at its value being either equal to or above 1.1 one should diagnose APS.
EFFECT: shortened terms of research.
1 ex, 4 tbl
SUBSTANCE: method involves analyzing symptoms manifesting initial disseminated intravascular blood coagulation syndrome danger like burn area, availability of upper air passages burn, shock with its severity degree taken into consideration, sepsis development; clinical manifestations of disseminated intravascular blood coagulation syndrome like lung, kidney, liver function insufficiency, cerebral dysfunction, local and multiple hemorrhages, thrombosis, infarction; homeostasis system laboratory analysis data, hyper- and hypocoagulation based on chronometry test data, number of blood platelets, fibrin-monomer complexes, D-dimers, activity of antithrombin III, C and S proteins, XIIa-dependent fibrinolysis plasminogen content, availability of injured erythrocytes, combinations of laboratory tests for recognizing disseminated intravascular blood coagulation syndrome. Each sign under consideration receives a number of points corresponding to its diagnostic significance and integral value is calculated DIBCSIV=(X1+X2+…+Xn)/n, where n is the number of signs taken into consideration. DIBCSIV value equal to 1.0-1.5 units shows physiological norm. The value being between 1.6 and 2.5 units, light disseminated intravascular blood coagulation syndrome is diagnosed. The value being between 2.6 and 3.5 units, disseminated intravascular blood coagulation syndrome of medium severity is diagnosed; 3.6-4.5 points to one heavy severity degree; 4.6 and greater indicates highly severe case of disseminated intravascular blood coagulation syndrome.
EFFECT: high accuracy and objectiveness in differentiating syndrome severity degrees.
FIELD: medicine, diagnostics.
SUBSTANCE: one should study blood components to detect anticoagulant-fibrinolytic activity. Moreover, patient's blood should be sampled: in whole blood one should detect the presence of affected erythrocytes and evaluate the quantity of thrombocytes, in plasma it is necessary to study the activity of antithrombin III, XIIa-dependent fibrinolysis, the content of soluble fibrin-monomeric complexes, in blood serum of the sample taken one should detect the concentration of urea, creatinine, sodium, albumin, total cholesterol and the activity of aspartate aminotransferase, moreover, one should calculate integral value of renal-hepatic deficiency, to put corresponding point for the degree of parameters under testing, then one should calculate integral value of disseminated intravascular clotting (IVDIC) and at its value being 6.3 U and more DIC-syndrome should be diagnosed, moreover, at IVDIC value ranged 6.3-10.1 U it is possible to diagnose latent DIC-syndrome, at 10.2-14.6 - subacute DIC-syndrome and at 14.7 and higher - acute DIC-syndrome should be concluded.
EFFECT: higher accuracy and efficiency of diagnostics.
4 ex, 2 tbl
FIELD: medicine, obstetrics.
SUBSTANCE: the present innovation deals with predicting disadaptive processes in women in dynamics of menstrual cycle. During menstrual cycle beginning since the 1st d to the 21st d one should detect the dynamics for alteration in coefficient of activity of syntoxic adaptation programs (CASAP), calculated by the following formula:
where CST - concentration of blood serotonin, AAT-III - activity of antithrombin III, Aaoa - total antioxidizing activity of plasma, CCD8 + - concentration of T-suppressors, Cad - concentration of blood adrenalin, Cα2MG - concentration of α2-macroglobulin, CMDA - concentration of malonic dialdehyde, CCD4 + - concentration of T-helpers. Moreover, normally CASAP value alters two-fold against the first day of the cycle - since 0.70 up to 1.40 on the 21st d of the cycle, at no alterations in CASAP value one should diagnose female disadaptive alterations leading to failed pregnancy. The innovation enables to perform diagnostics of disadaptive processes in women in dynamics of menstrual cycle followed by prognostic conclusion upon future pregnancy.
EFFECT: higher accuracy of diagnostics.
SUBSTANCE: method involves determining spontaneous blood platelets aggregation and one induced by adrenalin and collagen, thrombocytospecific peptides activity of β-thromboglobulin and thrombocytic factor 4 in blood plasma.
EFFECT: high accuracy of diagnosis.
SUBSTANCE: method involves determining coagulating blood viscosity values like reaction period r, thrombin constant K, maximum amplitude MA, time T for forming fibrin-thrombocytic blood clot, spontaneous blood platelets aggregation intensity Ar, retraction and spontaneous clot lysis total FA. The r being within 5-7 min, Ar from -2 to -6 relative units, K being within 4-6 min, MA within 500-700 relative units, T within 40-60 min and FA equal to 10-20%, low inflammatory process activity is considered to be the case. The r being less than 5 min, Ar equal to -8 to -12 relative units, T less than 40 min and FA less than 10% with no changes in K and MA being observed, inflammatory process activity in chronic glomerulonephritis case is considered to be of high severity degree.
EFFECT: high accuracy of diagnosis; enhanced effectiveness of treatment method selection.
FIELD: medicine, clinical neurology, neurosurgery.
SUBSTANCE: one should study both activation and aggregation of thrombocytes in blood of carotid artery, at the quantity of thrombocytic active forms being above 70% and the number of aggregated thrombocytes being above 9.0% one should predict the development of cerebral ischemic lesion along with stable focal neurological symptomatology, and at the quantity of thrombocytic active forms being below 30% and the number of aggregated thrombocytes being below 8.0% it is possible to predict positive dynamics in the course of the disease mentioned without developing cerebral ischemic lesion.
EFFECT: higher accuracy of prediction.
FIELD: medicine, clinical neurology, neurosurgery.
SUBSTANCE: one should study the level of von Willebrand's factor in patient's carotid artery blood. At its content being below 105% one should predict the development of repeated AICH. The innovation improved information value of testing due to possibility to obtain reliable prediction in latent period, as well.
EFFECT: higher accuracy of prediction.
2 ex, 1 tbl