Surface-active bleaching agent and dynamic ph

FIELD: chemistry.

SUBSTANCE: invention relates to a method of cleaning at least part of a surface and/or fabric, involving: optional steps for washing and/or rinsing the surface and/or fabric; bringing the surface and/or fabric into contact with a washing solution containing a perhydrolase enzyme and a substrate for said enzyme, where the initial pH of the washing solution is alkaline and the amount of the perhydrolase enzyme and substrate is sufficient to lower the pH of the washing solution to 6.5 or lower; and optional washing and/or rinsing the surface and/or fabric, where said contact takes place during the washing cycle, and where lowering of the pH of the washing solution improves efficiency of the component of the washing solution.

EFFECT: improved method for bleaching textile.

7 cl, 5 ex, 5 dwg

 

The technical FIELD TO WHICH the INVENTION RELATES

The present invention provides methods and compositions for dynamic control of pH, in particular for washing applications. In particularly preferred embodiments, the implementation found in detergent compositions used to remove stains from the surface of fabrics, including clothing. In some particularly preferred embodiments, the implementation of the present invention provides a combination of enzymes that provide dynamic pH control.

The prior art of the PRESENT INVENTION

Detergents and other cleaning compositions typically include a complex combination of active ingredients. For example, most cleaning products include surfactants, enzymes for cleaning, bleaching agents, additives for improving the washing steps, defoamers, suspendresume pollution funds dirt repellent tools, optical brighteners, softeners, dispersants, inhibiting migration of the dye compounds, abrasives, bactericides and fragrances. Despite the complexity of the currently used detergent, there are many dyes that are difficult to remove completely. In addition, there is often an accumulation of residues, which leads to discoloration (e.g., yellowing) and lowering aesthetics because n is a complete cleaning. These problems are exacerbated by the increasing use of low temperature washing (e.g., cold water) and shorter washing cycles. Moreover, many spots consist of complex mixtures of fibrous material, mainly including carbohydrates and derivatives of carbohydrates, fiber and components of the cell wall (for example, contamination of plant-based materials, wood, earth/clay and fruits). These spots cause significant difficulties in the preparation and application of the cleaning compositions.

In addition, colored clothing tends to wear out and lose the appearance. Of a specified loss of color due to wear in the washing process, especially in automatic washing and drying machines. Moreover, loss of strength tissue in the gap is the inevitable result of mechanical and chemical effects caused by the use, carrying, and/or washing and drying. There is therefore a need in the tool for successful and effective washing colored clothes so that this loss of appearance was minimal.

In General, despite improving the ability of the cleaning compositions, in the specified area of engineering remains a need for detergents that remove coloring matter, keep the color and appearance of the fabric and prevent the migration of the dye. Cu is IU, there remains a need for detergent compositions and/or compositions for the care of fabrics that will give and/or restores tensile strength, and also give the fabric the ability to not be flattened, not to slide and/or not take, and will also provide control of static electricity, the softness of the fabric will retain the desired color and the resistance of fabric to wear. In particular, there remains a need for specifying compositions, which are able to remove the painted components dyes, which are often left on the wash cloth. In addition, there remains a need for improved methods and compositions suitable for bleaching textiles.

The INVENTION

The present invention provides methods and compositions for dynamic control of pH, in particular for washing applications. In particularly preferred embodiments, the implementation found in detergent compositions used in the removal of stains from the surface of fabrics, including clothing. Dynamic control of pH during washing allows effective ingredients to fully use their potential in a suitable pH range, thus achieving the best cleaning effect. In addition, changes in the pH of the solution for washing (from a slightly alkaline pH to acidic pH) also destroys stains on the surface and remove some stains that are not removed when a higher value is s pH.

The present invention also provides compositions that contain a sufficient amount of at least one enzyme and at least one substrate for this enzyme, sufficient to reduce the pH of the washing solution to at least about pH 7 or below. In some particularly preferred embodiments, the implementation of the methods defined below (e.g., example 3) is used to determine the pH decreases. In some preferred embodiments, the implementation of the reduction in pH is approximately pH 6 or below. In some alternative embodiments, the implementation of an enzyme selected from hydrolases and oxidase. In some preferred embodiments, the implementation of hydrolase choose from peligrosas, hydrolases complex carboxylate ester hydrolases complex thioesters, hydrolases complex phosphate monoufia, hydrolases complex phosphate diesters, hydrolases simple thioethers, α-AMINOETHYLPIPERAZINE, peptidylarginine hydrolases, allumination hydrolases, dipeptidase, participatively, pepsin, pepsin B, rennin, trypsin, chymotrypsin A, chymotrypsin B, elastase, enterokinase, cathepsin C, papain, chymopapain, fitsin, thrombin, fibrinolizina, renin, subtilisin, aspergillomas A, collagenase, clostridiales B, kallikrein, Gast is exina, cathepsin D, bromelain, keratinase, chymotrypsin C, pepsin C, aspergillomas B, urokinase, carboxypeptidase A and B, amino peptidases, lipases, pectinesterase and chlorophylls. In some particularly preferred embodiments, the implementation of hydrolase contains at least one enzyme having pelikulang activity (for example, perhydrol defined below). In other additional embodiments, the implementation of an oxidase selected from alsoeasy, galactosidase, cellobiose, pianosounds, and carbony substrate contains an ester group. In some preferred embodiments, the implementation of the substrate containing the ester group is selected from ethyl acetate, triacetin, tributyrin, esters of neocola, ethoxylated esters of podlatat, formic acid, acetic acid, propionic acid, butyric acid, valerianic acid, Caproic acid, Caprylic acid, nonnovel acid, decanoas acid, dodecanol acid, myristic acid, palmitic acid, stearic acid and oleic acid. In other embodiments, the implementation of the substrate containing the ester group has the formula, R1Ox[(R2)m(R3)n]pwhere R1represents H or the group that contains the primary is tricou, tertiary or Quaternary amino group, and the group R1that contains the amino group, are selected from substituted or unsubstituted alkyl, heteroalkyl, alkenyl, quinil, aryl, alkylaryl, alkylglycerol and heteroaryl; or where R1contains from 1 to 50,000 carbon atoms, from 1 to 10,000 carbon atoms, or even from 2 to 100 carbon atoms; each R2represents alkoxylate group, in one aspect of the present invention each R2represents independently ethoxylate, propoxylate or butoxylate group; R3represents a group forming an ester having the formula: R4CO-, where R4can represent H, substituted or unsubstituted alkyl, alkenyl, quinil, aryl, alkylaryl, alkylglycerol and heteroaryl, in one aspect of the present invention R4may be a substituted or unsubstituted alkyl, alkenylphenol or alkenylphenol group containing from 1 to 22 carbon atoms, a substituted or unsubstituted aryl, alcylaryl, alkylglycerols or heteroaryl group containing from 4 to 22 carbon atoms, or R4may be a substituted or unsubstituted C1-C22alkyl group, or R4may be a substituted or unsubstituted C1-C12alkyl group; x PR is dstanley a 1, when R1represents H; when R1is not H, x is an integer that is equal to or less than the number of carbon atoms in R1, p is an integer that is equal to or less than x, m is an integer from 0 to 12, or even from 1 to 12, and n is at least 1. In other additional embodiments, the implementation of the composition specified below, contain, relative to the total weight of the composition, from about 0.01 to about 99.9 substrate containing ester group. In some preferred embodiments, the implementation of the compositions contain, in relation to the total weight of the composition, from about 0.1 to about 50 of the substrate containing the ester group. In other preferred embodiments, the implementation of the compositions also contain at least one source of hydrogen peroxide and/or hydrogen peroxide. In some preferred embodiments, the implementation of the compositions also contain at least one additional ingredient. In some particularly preferred embodiments, the implementation of at least one additional ingredient chosen from surfactants, additives for increasing the washing steps, chelating agents, inhibiting the transfer of dye funds, precipitating obivok, dispersants, additional enzymes, and enzyme stabilizers, catalytic materials, bleach activators, enhancers bleaching, pre-formed perkiset, polymeric dispersing agents, clay-based tools to remove stains/to prevent re-deposition of Britanov, defoamers, dyes, fragrances, means for imparting elasticity to the structure, fabric softeners, carriers, hydrotropes, additives and/or pigments.

The present invention also provides methods for cleaning at least part of the surface and/or tissue, including: an optional stage of the wash and/or rinse the surface and/or fabric; the bringing into contact of the surface and/or tissues with at least one of the compositions defined below, and/or detergent solution containing at least one of the compositions defined below; and optionally washing and/or rinsing the surface and/or fabric. In some preferred embodiments, the implementation of the pH of the washing solution decrease almost linearly. In other embodiments, implementation of the surface and/or fabric is exposed to the cleaning solution having a pH below about 6.5, for at least about 2 minutes.

The present invention also provides methods for cleaning at least part of the surface and/or the Kani, includes: optional stage wash and/or rinse the surface and/or fabric; the bringing into contact of the surface and/or tissues with at least one composition defined below, and/or detergent solution containing at least one composition defined below; and optionally washing and/or rinsing the surface and/or tissue, where the bringing into contact takes place during washing cycle. In some preferred embodiments, the implementation of the pH of the washing solution decrease almost linearly. In some particularly preferred embodiments, the implementation of the pH of the washing solution is reduced to 6.5 or below during the last 25-50% of the washing cycle. In additional embodiments, the implementation of the surface and/or fabric is exposed to the cleaning solution having a pH below about 6.5, for at least about 2 minutes.

DESCRIPTION of FIGURES

Figa-C are graphs showing the effect of pH on the efficiency of treatment with peracetic acid. Panel A presents the results for t-shirts, while panel B presents the results for pillow cases, and panel C presents the results for stains of tea.

Figure 2 represents a graph showing the curve of the titration of contaminated ballast.

Figa-C are diagrams showing parameters of substrate and enzyme, participation is relevant in achieving efficiency through dynamic pH. Panel A is a graph showing the pH profile processing, whereas panel B presents data for t-shirts, pillowcases, having hydrophobic pollution, and the average value of these data, and panel C presents data for wine, tea, having hydrophilic spots, and the average value of the specified data.

Figa-C are graphs showing the results of experiments conducted to determine the influence of subtrates and cleaning efficiency. Panel A is a graph showing the pH profile option processing, whereas panel B presents data for t-shirts, pillowcases, having hydrophobic pollution, and the average value of these data, and panel C presents data for wine, tea, having hydrophilic spots, and the average value of the specified data.

Figa-C are graphs showing comparison of cleaning efficiency of the detergent with dynamic pH and commercial detergents. Panel A is a graph showing the pH profile option processing, whereas panel B presents data for t-shirts, pillowcases, having hydrophobic pollution, and the average value of these data, and panel C presents data for wine, tea and having a hydrophilic spots, and the average value of the specified data.

Description of the INVENTION

Now the image is giving provides methods and compositions for dynamic control of pH, in particular for washing applications. In particularly preferred embodiments, the implementation found in detergent compositions used in the removal of stains from the surface of fabrics, including clothing. In some particularly preferred embodiments, the implementation of the present invention provides a combination of enzymes that provide dynamic control of pH during the entire wash cycle.

Unless otherwise noted, the practical application of the present invention includes conventional technologies that are widely used in molecular biology, Microbiology, protein purification, protein engineering, protein sequencing and DNA and in the field of recombinant DNA, which are within the competence in this field of technology. Such technologies are known to the specialist in the art and described in numerous texts and handbooks (see, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual. 2nd ed., Cold Spring Harbor, [1989]); and Ausubel et al., Current Protocols in Molecular Biology,[1987]). All patents, patent applications, articles and publications mentioned in the present description, both above and below, through this right is included in the present description by reference.

In addition, the titles presented in the present description, are not limitations of the various aspects or embodiments of the present invention that can be made on the note on the specification as a whole.

Accordingly, the terms defined directly below are more fully defined by reference to the specification as a whole. However, in order to simplify understanding of the present invention, several terms are defined below.

Definition

Unless otherwise specified in the present description, all technical and scientific terms used in this description have the same meaning as generally understood by the average expert in the field of technology to which belongs the invention. For example, Singleton and Sainsbury, Dictionary of Microbiology and Molecular Biology. 2d Ed., John Wiley and Sons, NY (1994); and Hale and Marham, The Harper Collins Dictionary of Biology. Harper Perennial, NY (1991) provides specialists in the art of the General dictionaries of many of the terms used in the present invention. Although all methods and materials similar or equivalent to those described in this description are used in the practical implementation of the present invention, the preferred methods and materials are described in the present description. Accordingly, the terms defined directly below are more fully described by reference to the specification as a whole. In addition, as used in the present description, the terms in the singular shall include a reference to the plural, unless the context clearly indicates otherwise. Unless otherwise indicated, nucleic acids are written to the left to right in the direction from 5' to 3'; amino acid sequences are written left to right in the direction from amino to carboxy respectively. It should be understood that this invention is not limited to particular methodology, protocols, and described reagents, because they can vary, depending on the context in which they are applied by an expert in the field of technology.

It is assumed that every maximum numerical limitation specified elsewhere in this specification, includes all lower numerical limitation, as if the data is lower numerical limitations were expressly stated in this description. Each minimum numerical limitation specified elsewhere in this specification, includes all higher numerical limitation, as if the data is higher numerical limitations were expressly stated in this description. Each numerical range as established elsewhere in this specification, includes all narrower numerical ranges that fall within this broader numerical range, as if all the data is narrower numerical ranges were directly specified in the present description.

As it is used in the present description, the term "dynamic pH" refers to the change in pH of the cleaning system in all cleaning, due to the action on minority who she least one enzyme on at least one substrate, present in the cleaning system. In particularly preferred embodiments, the implementation of the dynamic pH leads to improved treatment, such as increased cleaning efficiency of detergents.

As it is used in the present description, the term "bleaching" refers to the processing of the material (e.g., fabric, linen etc) or surface for a considerable period of time and under conditions corresponding pH and temperature, in order to achieve clarification (i.e. clenched) and/or cleaning material. Examples of chemicals that are suitable for bleaching, include, without limitation, ClO2H2O2, percolate, NO2etc.

As it is used in the present description, the term "disinfection" refers to the removal of contaminants from surfaces, as well as the inhibition or destruction of microbes on the surfaces of objects. It is not intended that the present invention limited to any particular surface, object, or remove air pollutant (nutrients) or microbes.

As it is used in the present description, the term "peligrosas" refers to an enzyme capable of catalyzing a reaction that leads to the formation of relatively large quantities of percolat suitable for applications such as cleaning, bleaching and disinfection. In osobennostiakh options implementation pergidrolya enzymes of the present invention give a very high ratio of peligrosa to hydrolysis. High ratio of peligrosa to the hydrolysis of a variety of these enzymes makes these enzymes suitable for use in very diverse applications. In additional preferred embodiments, the implementation of perhydrol of the present invention are characterized by the fact that they have different tertiary structure and primary sequence. In particularly preferred embodiments, the implementation of perhydrol of the present invention contain various primary and tertiary structures. In some particularly preferred embodiments, the implementation of perhydrol of the present invention include various Quaternary structure. In some preferred embodiments, the implementation of perhydrol of the present invention is peligrosas from M. smegmatis, whereas in alternative embodiments, implementation, perhydrol is a variant of this peligrosas, while in other embodiments, implementation peligrosas is a homologue of the specified peligrosas. In other preferred embodiments, the implementation of the Monomeric hydrolase design to get a multimeric enzyme that has the best pelikulang activity than the monomer. However, not intended that the present invention is limited to a specified certain perhidrolis from . smegmatis, certain options specified peligrosas or specific homologues of perhydrol presented in US04/40438 included in the present description by reference in its entirety.

As used in this description, "personal care products" refers to products used for cleaning, bleaching and/or dezynfytsyrovaly hair, skin, scalp, and teeth, including, without limitation, shampoos, body lotions, shower gels, topical moisturizers, toothpaste and/or other topical cleaners. In some particularly preferred embodiments, the implementation of these products find application in humans, whereas in other embodiments, the implementation of these products are used in animals other than humans (e.g., in veterinary applications).

As used in this description, "cleaning compositions" and "cleaning composition" refers to compositions that find use in the removal of undesirable substances from the cleaned objects, such as fabric, dishes, contact lenses, other solid substrates, hair (shampoos), skin (Soaps and creams), teeth (liquid for mouth rinses, toothpastes, and so This term includes any materials/substances selected for a particular type of desired chemical composition and form of the product (for example, the composition in the form of liquid, gel, is renal or aerosol), provided that the composition is compatible with perhidrolis and other enzymes used in the composition. The specific choice of materials, the cleaning composition can be easily made through consideration of the cleaned surface, object or tissue and the required conditions for purification during the application form of the composition.

These terms also refer to any compositions that are suitable for cleaning, whitening, dezynfytsyrovaly and/or sterilization of any object and/or surface. It is assumed that these terms include, without limitation, the detergent composition (e.g., liquid and/or solid detergents for Laundry and detergents for fine fabrics; compositions for cleaning hard surfaces, such as glass, wood, ceramic and metal table tops and Windows; vacuum cleaners; cleaners furnaces; air fresheners fabrics; fabrics softeners; and means for previously removing stains from textiles and clothes, as well as detergents for dishes).

In fact, the term "cleaning composition", as used in this description, includes, unless otherwise indicated, granular or powder-like universal or highly effective detergents, in particular cleaning detergents; liquid, gel or paste-like universal detergents, in particular the so-called high-performance liquid is barb (HDL); liquid detergents for fine fabrics; means for manual washing of dishes or soft products for dishwashing, in particular with a rich foaming; means for machine washing, including different types of tablets, granules, liquids and additives when rinsing for use at home and in institutions; liquid cleaning and disinfectant means, including antibacterial hand wash, cleaning bars, liquid for rinsing the mouth, cleaners, oral, shampoos for vehicles or carpet cleaners for baths; shampoos and conditioners for the hair; shower gels and foam baths and cleaners for metal; and an auxiliary cleaning ingredients such as bleach additives and "pencils for spots or tools for pre-processing.

As they are used in the present description, the term "cleaning composition" and "detergent composition" are used in connection with mixes that are expected to apply in the scrubbing medium for the purification of contaminated sites. In some preferred embodiments, the implementation of the specified term used in connection with the washing of fabrics and/or service (for example, detergents for washing"). In alternative embodiments, the implementation of the specified term refers to other detergents, such as used for cleaning utensils, Cutlery, etc. (for example, detergents for washing by the UDA"). It is not intended that the present invention be limited to any particular composition or detergent composition. In fact, it is assumed that, in addition to peligrosas, the term includes detergents that contain surfactants, transferase(s), hydrolytic enzymes, oxidoreductase, additives for improving the washing steps, bleaching agents, bleaching activators, podsinwowa tools and fluorescent dyes, anti-caking agents, destinatary, enzyme activators, antioxidants and solvents.

As used in this description, "improved efficiency" detergent is defined as an increased degree of purification sensitive to whitening pigments (for example, grass, tea, wine, blood, dirt, etc)defined by the usual measurement after a standard wash cycle. In some cases, the implementation of the enzymes of the present invention provide increased efficiency of the oxidation and removal of colored dyes and stains. In other embodiments, implementation of the enzymes of the present invention provide enhanced removal efficiency and/or bleaching of dyes. In other additional embodiments, the implementation of the enzymes of the present invention provide enhanced removal efficiency KRA is the yaschih substances and stains with a lipid basis. In other embodiments, implementation of the present invention provides increased efficiency in the removal of stains and dyes with utensils and other objects.

As it is used in the present description, the term "cleaning composition for hard surfaces" refers to detergent compositions for cleaning hard surfaces such as floors, walls, tiles, accessories for baths and kitchens, and the like. Such compositions are available in any form, including, without limitation, solids, liquids, emulsions, etc.

As used in this description, "composition for washing" refers to all forms of compositions for cleaning dishes, including, without limitation, granular and liquid forms.

As used in this description, "composition for cleaning fabrics" applies to all forms of detergent compositions for cleaning fabrics, including, without limitation, the form of granules, liquid and bar.

As used in this description, "textile" refers to woven materials, as well as staple fibers and filaments suitable for conversion to or use as yarns, woven, knitted and nonwoven fabrics. The term covers a yarn derived from natural and synthetic (e.g., synthetic) fibers.

As used in this description, "textile materials" represent is a General term for fibers, intermediates yarn, yarn, fabrics and products made from fabrics (for example, clothing and other products).

As used in this description, the "fabric" includes any textile material. Thus, it is assumed that this term includes clothing as well as fabric, yarn, fiber, non-woven materials, natural materials, synthetic materials, and any other textiles.

As it is used in the present description, the term "compatible" means that the materials of the cleaning composition does not reduce the enzymatic activity of perhydrol to such an extent that peligrosas is not as effective as required during normal use. Detailed examples of specific materials cleaning compositions are described later in the present description.

As used in this description, "an effective amount of the enzyme" refers to the amount of enzyme necessary to achieve the enzymatic activity required in a particular application. Such effective amounts can easily be determined by the average expert in the art and depend on many factors, such as the use of a particular variant of the enzyme, the cleaning application, the specific composition of the cleaning composition, and whether a liquid or dry (e.g., granular is, in the form of a bar) composition, and the like.

As used in this description, "a cleaning composition for fabrics include cleaning compositions for solid surfaces, compositions for washing and cleaning composition for personal hygiene (e.g., oral cleaning compositions, cleaning compositions for oral, personal cleaning composition and so on).

As used in this description, "oral cleaning composition" refers to means for care of teeth, toothpastes, gels, tooth, tooth powders, liquids for rinsing the mouth, aerosols for oral, gel, oral cavity, chewing gum, lozenges, dry spirits, tablets, biogels, prophylactic pastes, solutions for care of teeth and the like. Oral hygiene compositions which are used in combination with peredryagami of the present invention, are well known in the art (see, for example, U.S. patent No. 5601750, 6379653 and 5989526, all of which are included in the present description by reference).

As used in this description, "oxidizing agent" refers to a reagent that is capable of bleaching of any material. Oxidizing reagent is present in suitable for whitening quantity, pH and temperature. The term includes, without limitation, the plumage is of n hydrogen and percolate.

As used in this description, "acyl" is a General name of a group of organic acids, which are residues of carboxylic acids after removal of the group-OH (for example, ethanolbased, CH3CO-Cl) is acylchlorides formed from ethanoic acid, CH3COO-H). Names of individual acyl groups are formed by changing the "Wye" acid "-yl".

As it is used in the present description, the term "acylation" refers to a chemical transformation that produces a substitution on acyl (RCO-) group in the molecule, as a rule, instead of the active hydrogen group-OH.

As it is used in the present description, the term "transferase" refers to an enzyme that catalyzes the transfer of functional compounds in a number of substrates.

As used in this description, the "leaving group" refers to nucleophile, which is cleaved from the donor acyl substitution by another nucleophile.

As it is used in the present description, the term "enzymatic conversion" refers to the modification of the substrate in the intermediate product or the modification of the intermediate product into a final product by bringing into contact of the substrate or the intermediate product with the enzyme. In some embodiments, the implementation of the contact is carried out by direct who is Astia corresponding enzyme on the substrate or intermediate product. In other embodiments, the implementation of the bringing into contact includes the impact on the substrate or an intermediate product of an organism that expresses and/or secretes an enzyme, and/or metabolizes the desired substrate and/or intermediate product in the desired intermediate product and/or the final product, respectively.

As used in this description, the phrase "stability of detergent" refers to the stability of the detergent composition. In some embodiments, the implementation of the stability determines during the application of detergent, whereas in other embodiments, implementation of the specified term refers to the stability of the detergent composition during storage.

As used in this description, the phrase "resistance to proteolysis" refers to the ability of the protein (e.g. enzyme) to withstand proteolysis. It is not intended that the term is limited to the use of any specific protease to determine the stability of the protein.

As used in this description, "oxidation stability" refers to the ability of the protein to function under oxidative conditions. In particular, the term refers to the ability of the protein to function in the presence of various concentrations of H2O2and/or percolate. Stability under various oxidative conditions can be measured or what redstem standard procedures, well-known specialists in the field of technology and/or by the methods described in the present description. A significant change in the resistance to oxidation is evident from at least about 5% or greater increase or decrease (in most embodiments, it is preferable to increase) in the half-life of enzymatic activity compared to the enzyme activity observed in the absence of oxidative compounds.

As used in this description, "resistance to pH" refers to the ability of the protein to function at a certain pH. As a rule, most enzymes have a limited pH range in which they operate. In addition to enzymes that operate at medium pH (about pH 7), there are enzymes that are able to work in conditions of very high or very low pH. Stability at different pH can be measured by standard procedures known to experts in the art, and/or by the methods described in the present description. A significant change in the resistance to pH evident from at least about 5% or greater increase or decrease (in most embodiments, it is preferable to increase) in the half-life of enzymatic activity compared to f is rotatively activity at the optimum pH of this enzyme. However, it is not intended that the present invention is limited as some level of resistance to pH and pH range.

As used in this description, "thermal stability" refers to the ability of the protein to function at a certain temperature. As a rule, most enzymes have a limited temperature range in which they operate. In addition to enzymes that operate at intermediate temperatures (e.g. room temperature), there are enzymes that are able to work in conditions of very high or very low temperatures. Temperature stability can be measured by known procedures or by methods described in the present description. A significant change in thermal stability is evident from at least about 5% or greater increase or decrease (in most embodiments, it is preferable to increase) in the half-life of catalytic activity of the mutant under the influence of temperature, different (i.e. higher or lower)than the optimal temperature for enzymatic activity. However, it is not intended that the present invention is limited as some level of temperature stability and temperature range.

As it is used in this description is, the term "chemical stability" refers to the stability of the protein (e.g. enzyme) in relation to chemicals that adversely affect its activity. In some embodiments, the implementation of such reagents include, without limitation, hydrogen peroxide, percolate, anionic detergents, cationic detergents, nonionic detergents, chelating agents, etc. However, it is not intended that the present invention is limited as some level of chemical stability and range of chemical stability.

As used in this description, the phrase "changing substrate specificity" refers to changes in the substrate specificity of the enzyme. In some embodiments, the implementation of the change in substrate specificity is defined as the difference between the attitude of the Kcat/Kmobserved for the enzyme, compared with the variants of the enzyme or other enzyme compositions. Enzyme-substrate specificity varies depending on the test substrate. The substrate specificity of the enzyme was determined by comparing demonstrate catalytic efficiency with different substrates. These detection methods find particular use in determining the effectiveness of the mutant enzymes, since, as a rule, it is desirable to obtain the variants of enzymes, which is exhibit higher ratios for certain interest substrates. For example, pergidrolya enzymes of the present invention is more efficient in the production of percolat of the ester substrate than the enzymes currently used for cleaning, bleaching and dezynfytsyrovaly. Another example of the present invention is peligrosas with lower activity in the degradation of percolate compared with wild type. Another example of the present invention is peligrosas with higher activity in relation to the more hydrophobic acyl groups than acetic acid. However, it is not intended that the present invention is limited as any appropriate substrate composition, and a particular substrate specificity.

As used in this description, the phrase "independently selected from the group consisting of..." means that those groups or elements that are selected from a specified group Markush may be the same, may be different, or any mix of elements, as shown in the following example:

With respect to chemical composition, the term "substituted"as used in this description indicates that the organic composition or radical to which is applied the term:

(a) steel unsaturated due to the elimination of at least one element or radical; or

(b) at least Odie is hydrogen in the compound or radical substituted by the group containing one or more atoms (i) carbon, (ii) oxygen, (iii) sulfur (iv) nitrogen or (v) halogen; or

(c) both (a)and (b).

Group, which may replace hydrogen as described in (b) immediately above, which contain only carbon atoms and hydrogen, represent a hydrocarbon group, including, without limitation, alkyl, alkenylphenol, alkenylphenol, alkylamino, cycloalkyl, phenyl, alkylphenyl, naftalina, untilnow, phenanthroline, fluorinol, steroids, and combinations of these groups with each other and with polyvalent hydrocarbon groups such as Allenova, alkylidene and alkylidyne group. Groups containing oxygen atoms, which may replace hydrogen as described in (b) immediately above include, without limitation, containing hydroxyl, acyl or ketone, ether, epoxy, carboxyl and ester groups. The group consisting of sulfur atoms, which may replace hydrogen as described in (b) immediately above include, without limitation, groups, sulfur-containing acids and acid esters, thioester group, mercaptopropyl and tokyograph. Groups containing nitrogen atoms, which may replace hydrogen as described in (b) immediately above include, without limitation, an amino group, a nitrogroup, isopropy, synthesis group, Minnie group, sidegroup, isocyanate group, ceanography and nitrile groups. The group consisting of halogen atoms, which may replace hydrogen as described in (b) immediately above include the group chlorine, bromine, fluorine, iodine, and any of the above groups in which hydrogen or alkyl side group substituted by the group of halogen, so that a stable substituted group.

It should be understood that all of the above groups of (b)(i) through (b)(v) can be substituted for each other or monovalent substitution, or by loss of hydrogen at a polyvalent substitution, so that there is formed another monovalent group which can substitute for hydrogen in an organic compound or radical.

As they are used in the present description, the terms "purified" and "isolated" refer to the removal of contaminants from a sample. For example, an enzyme purified by removal of contaminating proteins and other compounds contained in the solution or medication that are not of interest by the enzyme. In some embodiments, the implementation of interest recombinant enzymes Express in bacterial or fungal cells, hosts, and these of interest recombinant enzymes purify by removing other components of the host cells; by this item is Ozernoe content of polypeptides of interest recombinant enzyme in the sample increases.

As used in this description, "protein of interest" refers to a protein (e.g. enzyme or of interest to the enzyme"), which is subjected to analysis, identify and/or modify. In the present invention are the use of natural and recombinant proteins.

As used in this description, "protein" refers to any composition consisting of amino acids and defined as a protein by an expert in the field of technology. The terms "protein", "peptide" and " polypeptide in the present description are used interchangeably. Where the peptide is a portion of the protein, specialists in the art can understand the meaning of this term from the context.

As used in this description, functionally and/or structurally similar proteins are considered as "related proteins". In some embodiments, the implementation of these proteins originate from different genera and/or species, including the difference between classes of organisms (e.g. bacterial protein and fungal protein). In some embodiments, implementation, these proteins originate from different genera and/or species, including the difference between classes of organisms (e.g., a bacterial enzyme and fungal enzyme). In additional embodiments, the implementation of related proteins originate from the same species. In fact, n is assumed what is the present invention limited to related proteins from any particular source (s). In addition, the term "related proteins" encompasses homologues of the tertiary structure and the primary sequence homologs (e.g., enzymes of the present invention). In other embodiments, implementation of the specified term encompasses proteins that are immunologically cross-react. In some preferred embodiments, the implementation of the related proteins of the present invention show a very high ratio of peligrosa to hydrolysis.

Cleaning and detergent compositions

Detergent compositions of the present invention are offered in any suitable form, including, for example, in the form of liquid solvent, granules, emulsions, gels and pastes. When using solid detergent composition, the detergent is preferably formed in the form of granules. Preferably, the granules are formed so that they additionally contain protective agent (see, for example, the application U.S. serial No. 07/642669, registered on January 17, 1991, is hereby incorporated into this description by reference). Similarly, in some embodiments, the implementation of the granules formed so that they contained materials to decrease the dissolution rate of the pellets in the environment for washing (see, for example, U.S. patent No. 5254283 included the present description by reference in its entirety). In addition, pergidrolya enzymes of the present invention are used in compositions in which the substrate and the enzyme are present in the same granule. Therefore, in some embodiments, the implementation efficiency of the enzyme is increased by providing a high local concentration of enzyme and substrate (see, for example, the publication of the patent application U.S. US2003/0191033 included in the present description by reference).

Many of the enzymes and variants of enzymes that find use in the present invention, suitable for a variety of detergent compositions. A number of known components are suitable surfactants suitable for azannyh compositions. These include nonionic, anionic, cationic, anionic or zwitter-ionic detergents (see, for example, U.S. patent No. 4404128 and 4261868). Suitable detergent composition is described in U.S. patent No. 5204015 (previously included as a reference). Experts in the art are familiar with various compositions, which are used as cleaning compositions.

As indicated in the present description, in some preferred embodiments, the implementation of the detergent compositions of the present invention uses a surface-active agent (i.e. surfactant, including anionic, not the district and impolitically surfactants, well-known for their use in detergent compositions. Some surfactants suitable for use in the present invention are described in British patent application No. 2094826 A, is incorporated into this description by reference. In some embodiments, the implementation of the present invention apply a mixture of surface-active substances.

Suitable anionic surfactants for use in detergent compositions of the present invention include unbranched or branched alkylbenzenesulfonate; sulfates alilovic or alkenilovyh ethers having an unbranched or branched alkyl group or alkeneamine group; alkyl or alkanesulfonyl; reincorporate; alkanesulfonyl and the like. Suitable protivin for anionic surfactants include alkali metal ions such as sodium and potassium; alkaline earth metal ions such as calcium and magnesium; ammonium ion; and alkanolamine having from 1 to 3 alkanols groups with carbon number 2 or 3.

Impolitically surfactants which find use in the present invention include sulfonates, Quaternary ammonium salts, impolitically surfactants of betaine type, and the like. Such impolitically surface is about-active substances have both positive and negatively charged groups in the same molecule.

Nonionic surfactants, which are used in the present invention, typically include polyoxyalkylene ethers, and higher alkanolamide fatty acids or their adducts with acceleratedly, complex glycerin monetary fatty acids and the like.

In some preferred embodiments, the implementation of the surfactant or mixture of surfactants included in the detergent compositions of the present invention, is provided in an amount of from about 1 percent by weight to about 95 percent by weight of the entire detergent composition and preferably from about 5 percent by weight to about 45 percent by weight of the entire detergent composition. In various embodiments, the implementation in the composition of the present invention include numerous other components. Many of them are described below. It is not intended that the present invention is limited to these specific examples. In fact, it is anticipated that additional compounds will find use in the present invention. The description below only illustrates some optional components.

Proteins, in particular peligrosas and/or other(s) enzyme(s) of this image is placed usually include known poroshkoobraznye and liquid detergents, having a pH between 3 and 12.0 in the amount of from about 0.001 to about 5% (preferably from 0.1% to 0.5%) by weight. In some embodiments, the implementation of these detergent cleaning compositions also include other enzymes (e.g. protease, amylase, mannanase, peroxidase, oxidoreductase, cellulase, lipase, chitinase, pectinase, pectinase, xylanase and/or endoglycosidase), as well as additives to enhance the cleaning action and stabilizers. In fact, provided that any enzyme hydrolysing activity will be used in pure form and/or in combination with other enzymes in the present invention.

It is easy to understand that in addition to conventional cleaning compositions options peligrosas of the present invention are used for any purpose for which the use of native or wild-type enzyme. Therefore, such variations can be used, for example, in applications of solid and liquid Soaps, compositions for washing dishes, applications for cleaning surfaces, solutions or products for cleaning contact lenses, recycling, applications for textiles, disinfectants, skin care, oral care mouth, hair care, etc. in fact, it is not expected that any options perhydrol the present invention limited to any specific application. the example option peligrosas of the present invention may have, in addition to reduced allergenicity, increased efficiency in washing compositions (compared with perhidrolis wild-type or unmodified).

Adding protein to conventional cleaning compositions does not create any special restrictions apply. In other words, any temperature and pH suitable for the detergent, are also suitable for the present compositions, provided that the pH is in the range in which the enzyme(s) active (active), and the temperature below the denaturation temperature of the protein. In addition, proteins of the present invention find use in cleaning, bleaching and disinfectant compositions without detergents, again or in pure form or in combination with a source of hydrogen peroxide, ester substrate (for example, or added to, or inherent in the system that is used, such as with coloring substances, which contain esters containing esters etc), other enzymes, surfactants, additives for improving the washing steps, stabilizers, etc. in fact, it is not intended that the present invention be limited to any particular structure or application.

DETAILED DESCRIPTION of the PRESENT INVENTION

The present invention provides FPIC who would and compositions for dynamic control of pH, in particular for washing applications. In particularly preferred embodiments, the implementation found in detergent compositions used in the removal of stains from the surface of fabrics, including clothing. In some particularly preferred embodiments, the implementation of the present invention provides a combination of enzymes that provide dynamic control of pH. In fact, it is assumed that any enzyme hydrolysing or pergidrolya activity will be used in pure form and/or in combination with other enzymes in the present invention.

A well-known method of washing fabrics in automatic washing machines. Standard operating procedure automatic washing machine includes at least one wash cycle, at least one cycle of extraction, which removes a significant portion of the wash solution from the wash cycle, the last cycle of rinse and final spin cycle.

Cleaning products (for example, surfactants and additives to enhance the cleaning action of the detergent) are usually added to the washing machine drum during the wash cycle and/or Polskaya that they took part in the removal of stains and dyes from fabrics. However, sometimes additional materials, such as tools for better fabric care (for example, softeners, conditioners sensations from the fabric to the touch, the tion against sinemasi etc), add to the load of the machine during the rinse cycle, instead of a wash cycle, in order to avoid interference with components piastowski in the washing solution. Some of these materials (for example, fragrances, products for whitening, such as tools for better fabric care and/or means for removing stains) applied to the tissue, in order to get the maximum positive effect. In some cases it is desirable to maximize the possible number of these materials remaining on the fabric.

the pH of the aqueous washing solution during the beginning of the wash cycle, usually high, usually above 7, and, in most cases, at least 9. In fact, he often lies in the range from 10.5 to 12.5, and sometimes even higher. However, in some embodiments, implementation of the present invention the desired end pH less than or equal to 6. Because of the different nature of additives, usually made in a cycle of washing and/or rinsing, and extracting most of the washing solution, the pH of the rinse cycle, as a rule, lower than in the cycle of washing, but, as a rule, it is not below 7. Although used cycles rinse with pH below pH 7, it is not common practice. The process of automatic washing machines are special requirements, it is normal to make complex detergent composition in the washing cycle, and is common on the part of various types of tissues in one washing machine load.

You need to compositions for washing clothes were technically and economically feasible, and acceptable to the consumer. In particular, the removal of fatty coloring agents and/or bleach dyes is a constant challenge for developers of detergents for washing. Although this area needs improvement, the types of components in compositions for washing clothes, which significantly improve efficiency, demonstrate a tendency to treat the most expensive components (such as bleaches). The present invention provides compositions and methods for improving the efficiency of detergents for clothes best for the value.

In addition, the present invention provides compositions and methods suitable for effective cleanup of contaminated sites. The problem that occurs when washing in an automatic washing machine, is the gradual deposition of residues on the fabrics after a number of washings. In addition, while wearing appears a significant number of spots from the body and the environment, remaining in the tissues, which in the future are spot residues. These residues often result in fading dark-colored fabrics and/or the emergence of a "dirty" appearance of white and/or other light colored fabrics. Specified on the situation residue also makes more difficult the removal of coloring matter from the surfaces of tissues. The present invention finds application for more effective, than used in the present compositions, treatment of the contaminated tissues and their treatment.

Since the optimum pH values of the various active components in detergents for Laundry vary considerably, as well as pH-dependent efficiency of cleaning contaminated tissues, necessary arrangements that can work effectively in a wide range of pH, cleaning soiled fabric. Pergidrolya the enzyme of the present invention, used in some embodiments to implement the present invention finds application in education percolate bleaches and lowering the pH of the acid of the ester substrates. In some embodiments, the implementation of data ester substrates are present in patches, while in other embodiments, the implementation of them add to the composition and/or loading machine. In particularly preferred embodiments, the implementation of surface-active esters adsorb on the surface of the fabric and stain, in order to obtain the target whitening. Therefore, enzymes, such as proposed by the present invention which have a high affinity to surfaces stains and/or tissue, facilitate limited surface formation of bleach and/or acid. Formulations oblad the matter of medium alkalinity, account for the higher activity and solubility of certain components (for example, parkinlot), with a pKa of approximately 8,2 and surfactants. Hydralazine cleavage of esters produces acid, which lowers pH, dissolving oily residues and improving the efficiency of the erase components with optimal activity at acidic pH values.

In some particularly preferred embodiments, the implementation of perhydrol, surface-active esters, triacetin, hydrogen peroxide and minimum surface-active basis are used in the cleanup of contaminated products. In some embodiments, the implementation of the spots in the first place include spots from the body. In some embodiments, the implementation of the contaminated cloth titrated, so get the appropriate buffer system, to provide an alkaline pH, but with sufficient capacity to accommodate the decrease in pH due to the enzymatic production of acid. As indicated in the present description, tests were conducted efficiency in mini washing machines in the middle for North American conditions. The composition of the enzymatic bleaching and dynamic pH, proposed by the present invention, works better than commercial liquid detergent with products bearing the stains from the body. In some preferred embodiments, made what I adding enzyme delayed for 5 minutes (ie. hydrolase add after 5 minutes, a 12-minute wash cycle), while the substrate and perhydrol add to the load of the machine at the beginning of the wash cycle.

In some embodiments, the implementation of the present invention finds application in the enzymatic formation of percolat of ester substrates and hydrogen peroxide. In some preferred embodiments, the implementation of the substrates are selected from one or more of the following: formic acid, acetic acid, propionic acid, butyric acid, valerianic acid, Caproic acid, Caprylic acid, nonnovel acid, decanoas acid, dodecanol acid, myristic acid, palmitic acid, stearic acid and oleic acid. It is essential that the present invention provides a tool for effective cleaning, bleaching and dezynfytsyrovaly in a wide range of pH and temperature. In some embodiments, the implementation of the pH range used in said formation, is 4-12. In alternative embodiments, the implementation of the used temperature range is between 5° and 90°C. the Present invention offers advantages over currently used systems (see, for example, European patent application 87-304933 .9), in which the bleaching is possible at the optimum pH for the oxidation of Perkele is, providing also whitening at neutral pH, acidic pH and at low temperatures. Despite the fact that the present invention described in the present description the most detail regarding the care of the linen and fabrics, it is not intended that the present invention is limited to these applications. In fact, the present invention finds application in various settings, particularly in those in which bleaching using perkiset and/or hydrogen peroxide, preferably under conditions of dynamic pH, including, without limitation, washing, fabric processing, use in personal hygiene, disinfection and cleaning of hard surfaces.

Historically perborate sodium, and in a closer time percarbonate sodium, used as compounds for bleaching, in particular in detergents for washing. This compound is rapidly degraded in aqueous solution, giving hydrogen peroxide (H2O2), which is the active bleaching agent. Because perborate sodium more active at temperatures above 80°C and less active in the temperature range of 40-60°C (i.e. the temperature of the washing, which became the most widely preferred in the 1950s), in detergents for Laundry, which contained perborate sodium, introduced the bleaching activators. In fact, most detergents for clothes contain activators is otbelivanie. These activators are compounds with O - or N-linked acetyl groups, which can react with strong nucleophilic hydroperoxides, giving peroxyoctanoic acid. Because reactive substance it hydroperoxide, alkaline pH is essential for the effective transformation of these activators in percolate. Peroxidasa acid decomposes in alkaline environment, forming singlet oxygen (see Hofmann et al., J. Prakt. Chem., 334:293-297 [1992]).

Hydrogen peroxide is a particularly effective bleach at high temperatures (e.g., >40°C) and pH (>10), conditions that are typically used when washing fabrics in some settings. However, as indicated above, wash in cold water is applied more and leads to less efficient whitening by H2O2than when using hot water. To overcome this disadvantage low temperature, detergent compositions typically include amplifiers whitening, such as TAED (N,N,N, N'-tetraacetylethylenediamine), NOBS (nonanoyloxybenzenesulfonate) etc. These amplifiers connect with H2O2to create percolate, which are more effective than only H2O2. Although this improves the ability of the detergent to the bleaching, the reaction TAED effective only approximately the additional 50% since only two of the four acetyl groups TAED become percolate. In addition, the transformation of TAED in peracetic acid hydrogen peroxide is effective only at alkaline pH and high temperatures. Therefore, the reaction TAED is not optimized for use in all applications bleaching (for example, using a neutral or acidic pH and cold water). The present invention provides means for overcoming the disadvantages of the use of TAED. For example, the present invention finds use in applications with cold water, and use a neutral or acidic pH levels. Moreover, the present invention offers a means of education parkinlot of hydrogen peroxide with a high ratio of peligrosa to hydrolysis.

Moreover, pergidrolya and/or hydralazine enzymes of the present invention are active on various substrates-acyl donors, and active at low concentrations of substrate, and provide a means for effective peligrosa due to the high ratio of percolate:acid. In fact, it became clear that the higher ratio of peligrosa to hydrolysis are preferred for applications bleaching (see, for example, U.S. patent No. 5352594, 5108457, 5030240, 3974082 and 5296616, all of them are included in the present description by reference). In some preferred option is the implementation of pergidrolya enzymes of the present invention provide a ratio of peligrosa to hydrolysis above 1. In some particularly preferred embodiments, the implementation pergidrolya enzymes provide the ratio of peligrosa to hydrolysis above 1 and are used in bleaching.

In addition, it was demonstrated that it is active in a widely used detergent compositions (e.g., Ariel Futur, WOB, and so on). Thus, the present invention offers numerous advantages for various cleanup options.

As indicated above, the key components in getting parkinlot by enzymatic peligrosa are enzyme ester substrate and hydrogen peroxide. The hydrogen peroxide may be either added directly to the party, or be produced continuously "in situ." Currently in washing powders use add H2O2the party in the form of salts of percarbonate or perborate, which spontaneously decompose to H2O2. Pergidrolya enzymes of the present invention find application in the same way add to the party with washing powder as the source of H2O2. However, these enzymes are also used with any other suitable source of H2O2including educated chemical, electrochemical and/or enzymatic means. Examples of chemical sources are percarbonate and perborate, by mentioning the saints above, then as an example of the electrochemical heat source is an item filled with oxygen and hydrogen, and enzymatic example includes obtaining H2O2in the reaction of glucose with glucose oxidase. The following equation provides an example of the coupled system, which finds use with the present invention.

Glucose oxidase

Glucose + H2O --------------------> gluconic acid + H2O2

+

Peligrosas

H2O2+ ester substrate ----------> alcohol + percolate

This system produces acid (acid), which leads to lowering the pH of the system. It is not intended that the present invention limited to any specific enzyme, as any enzyme that produces H2O2and the acid with a suitable substrate used in the methods of the present invention. For example, lacticacid species Lactobacillus known that they create H2O2of lactic acid and oxygen, are used with the present invention. In fact, one advantage of the methods of the present invention is that the acid production (e.g., gluconic acid in the above example) lowers the pH of the basic solution until the pH range in which percolate most effective in bleaching (i.e. near or below pKa). Other enzymes (for example the EP, the carbohydrate oxidase, alcoholecstasy, etilenvinilatsetata, glycerolipids, oxidase amino acids and so on)that can generate hydrogen peroxide, are also used with ester substrates, providing percolate in combination with pergidrolya enzymes of the present invention. Enzymes that can produce acid from substrates without the production of hydrogen peroxide, also find use in the present invention. Examples of such enzymes include, without limitation, esterase, lipase, phospholipase, cutinase, protease. In some preferred embodiments, the implementation of the ester substrate selected from one or more of the following acids: formic acid, acetic acid, propionic acid, butyric acid, valerianic acid, Caproic acid, Caprylic acid, nonnovel acid, decanoas acid, dodecanol acid, myristic acid, palmitic acid, stearic acid and oleic acid. Thus, as described in the present description, the present invention offers certain advantages over the currently used methods and compositions for the development and application of detergents and various other applications.

Activity peligrosas

Many years used enzymes derived the C microorganisms. In fact, there are numerous biological catalysts known in the art. For example, U.S. patent No. 5240835 (incorporated in the present description by reference) provides a description of the activity transacylase obtained from C. oxydans and receive it. In addition, U.S. patent No. 3823070 (incorporated in the present description by reference) provides a description of Corynebacterium which produce certain fatty acids of the n-paraffin. U.S. patent No. 4594324 (incorporated in the present description by reference) provides a description of Melhylcoccus capsulatus, which oxidizes alkenes. Additional active agents known in the art (see, for example, U.S. patent No. 4008125 and 4415657; they are both included in the present description by reference). EP 0280232 describes the application of the enzyme C. oxydans in the reaction between diola and a complex ester of acetic acid with getting monoacetate. Additional references describe the use of enzyme C. oxydans for obtaining chiral hydroxycarbonate acid from prehiring diol. Additional details related to the activity transacylase C. oxydans, as well as to culture C. oxydans, production and purification of the specified enzyme suggested by U.S. patent No. 5240835. Thus, the ability of the specified enzyme to transesterification, mainly with the use of acetic acid was known. However, the fact that Yes the hydrated enzyme can conduct the reaction peligrosa was completely unexpected. Was even more unexpected that these enzymes show very high efficiency in the reactions of peligrosa. For example, in the presence of tributyrin and water, the enzyme produces butyric acid, whereas in the presence of tributyrin, water and hydrogen peroxide, the enzyme produces mainly permalloy acid and very little butyric acid. Mentioned high ratio of peligrosa to hydrolysis is a unique property exhibited by the enzymes of the present invention class peligrosas, and it is a unique characteristic not exhibited previously described by lipases, cutinase or esterases.

Peligrosas of the present invention are active in a wide range of pH and temperature, and accepts a wide range of substrates for transferring acyl. The acceptors include water (hydrolysis), hydrogen peroxide (perhydrol) and alcohols (classical transferring acyl). To measure peligrosa enzyme incubated in the desired buffer at a certain temperature with the substrate-complex ester in the presence of hydrogen peroxide. Typical substrates used for measuring peligrosa will include esters such as ethyl acetate, triacetin, tributyrin, ethoxylated esters of neodermata and others. In addition, the wild-type enzyme hydrolyzes complex nitrophenolate esters of acids to the short circuit. The latter are common substrates for measuring the concentration of the enzyme. Percolate and acetic acid can be measured using the analysis described in the present description. Hydrolysis nitrophenylamino ether are also described.

Although the primary example used during development of the present invention is peligrosas M. smegmatis, any peligrosas obtained from any source, which turns ester mainly in percolate in the presence of hydrogen peroxide used in the present invention. In some particularly preferred embodiments, the implementation of perhydrol described in US04/040438 (WO 05/056782), which is incorporated into this description by reference in its entirety.

In some preferred embodiments, the implementation of the present invention with pergidrolya and/or hydralazine enzymes of the present invention is applied esters containing an aliphatic and/or aromatic carboxylic acids and alcohols. In some preferred embodiments, the implementation of substrates-esters are selected from one or more of the following esters of acids: formic acid, acetic acid, propionic acid, butyric acid, valerianic acid, Caproic acid, Caprylic acid, nonnovel acid, decanoas acid, dodecanol is islote, myristic acid, palmitic acid, stearic acid and oleic acid. In additional embodiments, the implementation of triacetin, tributyrin, esters of neodol and/or ethoxylated esters of neodol serve as acyl donors for the education of percolate/acid.

In some preferred embodiments, the implementation of the present invention with pergidrolya and/or hydralazine enzymes in detergent compositions of the present invention is applied esters containing an aliphatic and/or aromatic carboxylic acids and alcohols. In some preferred embodiments, the implementation of the substrates are selected from one or more of the following esters of acids: formic acid, acetic acid, propionic acid, butyric acid, valerianic acid, Caproic acid, Caprylic acid, nonnovel acid, decanoas acid, dodecanol acid, myristic acid, palmitic acid, stearic acid and oleic acid. Thus, in some preferred embodiments, the implementation offers a detergent containing at least one peligrosas and/or hydrolase at least one source of hydrogen peroxide and at least one ester of the acid.

The activity of hydrolases

In addition to peligrosas described the Oh in the present description, in the present invention find application in various hydrolases, including, without limitation, hydrolase complex carboxylate esters, hydrolase complex thioesters, hydrolase complex phosphate monoamino and hydrolase complex phosphate diesters, acting on ester bonds; hydrolase simple thioethers, which operates on essential communication; and α - AMINOETHYLPIPERAZINE, peptidylarginine hydrolase, acylaminoalkyl hydrolase, dipeptidase, participatiegraad, acting on peptide bond. This hydrolase(s) used in pure form or in combination with perhidrolis. Among them, preferred are hydrolase complex carboxylate esters and participatiegraad. Suitable hydrolases include: (1) protease, prinadlezashie to the class of participatiegraad (for example, pepsin, pepsin B, rennin, trypsin, chymotrypsin a, chymotrypsin B, elastase, enterokinase, cathepsin C, papain, chymopapain, fitsin, thrombin, fibrinolizin, renin, subtilisin, aspergillomas A, collagenase, clostridiales B, kallickrein, gastoxin, cathepsin D, bromelain, keratinase, chymotrypsin C, pepsin C, aspergillomas B, urokinase, carboxypeptidase A and B and aminopeptidase); (2) hydrolase complex carboxylate esters, including carboxylesterase, lipase, pectinesterase, chlorophy the manhole; and (3) enzymes having a high ratio of peligrosa to hydrolysis. Among them especially effetive lipase and esterase, which show a high ratio of peligrosa to hydrolysis, and protein engineering esterase, cutinase and lipase, using primary, secondary, tertiary and/or Quaternary structural features of perhydrol of the present invention.

This hydrolase is introduced into the detergent composition in the required amount in accordance with the task. Preferably it should be entered in the amount of from 0.00001 to 5% by weight and more preferably from 0.02 to 3 percent by weight. This enzyme should be used in the form of granules, cleannig of the raw enzyme in pure form or in combination with other enzymes and/or components of the detergent composition. The granules of the untreated enzyme used for in such quantity that the purified enzyme is from 0.001 to 50 percent by weight of the granules. Pellets are used in amounts of from 0.002 to 20 and preferably from 0.1 to 10 percent by weight. In some embodiments, the implementation of the granules are so that they sondergut tool that protects the enzyme, and the material that slows the dissolution (i.e. material, which regulates the dissolution of the granules during the application).

When using peligrosas of the present invention sostav the et between about 0.01 ppm and 100 ppm in the washing solution. In some preferred embodiments, the implementation of perhydrol is present in a concentration of between approximately 0.1 and 10 ppm

The oxidase activity

Washing composition of the present invention includes a carbohydrate oxidase, i.e. the enzyme that catalyzes the oxidation of carbohydrate substrates, such as carbohydrate monomers, dimers, trimers or oligomers, and restores molecular oxygen, producing hydrogen peroxide.

Suitable carbohydrate oxidase include carbohydrate oxidase selected from the group consisting of alsoeasy (IUPAC classification EC 1.1.3.9), galactosidase (IUPAC classification EC 1.1.3.9), cellobioside (IUPAC classification EC 1.1.3.25), pianosounds (IUPAC classification EC 1.1.3.10), carbothioamides (IUPAC classification EC 1.1.3.11) and/or exotoxicity (IUPAC classification EC 1.1.3.5), glucose oxidase (IUPAC classification ECl.1.3.4) and mixtures thereof. In fact, it is envisaged that any suitable oxidase (i.e., which follows the equation

Enzyme + substrate -> acid and H2O2) finds use in the present invention.

A qualified professional with the enzymes classified as EC 1.1.3._, KF 1.2.3._, EC 1.4.3._ and KF 1.5.3._, understand that similar classes of enzymes on the basis of the recommendations of the Nomenclature Committee of the International Union of biochemistry molekulyarnoi biology (IUBMB) applicable in the present invention.

In some embodiments, implementation of the preferred carbohydrate oxidase include alasoorituse and/or galactosidase, is preferred alasoorituse because of its broader substrate specificity. Alasoorituse active against all mono-, di-, tri -, and oligo - carbohydrates, such as D-arabinose, L-arabinose, D-cellobiose, 2-deoxy-D-galactose, 2-deoxy-D-ribose, D-fructose, L-fucose, D-galactose, D-glucose, D-glycero-D-Gulo-heptose, D-lactose, D-lyxose, L-lyxose, D-maltose, D-mannose, melezitose, L-melibiose palatinose, D-raffinose, L-rhamnose, D-ribose, L-sorbose, stachyose, sucrose, D-trehalose, D-xylose and L-xylose.

In some particularly preferred embodiments, implementation of the preferred carbohydrate oxidase is alasoorituse described in WO99/31990, and the polypeptide proizoditsya Microdochium nivale CBS 100236, or has the amino acid sequence described in SEQ ID NO:2, or is similar to it. In addition, oxidase, which have a much broader substrate specificity and are therefore able to remove carbohydrates more efficiently and remove a wider range of carbohydrates that are used in the present invention. For example: galactosidase acts on D-galactose, lactose, melibiose, raffinose and stachyose; cellobioside acts on cellobiase, and is also on allocstring, lactose and D-mannose; pianosounds effect on D-glucose and D-xylose, L-sorbose and D-glucose-1,5-lactose; carbothioamides acts on L-sorbose, and D-glucose, D-galactose and D-xylose; and hexosaminidase effect on D-glucose and D-galactose, D-mannose, maltose, lactose and cellobiose.

Suitable exotoxicity include described in WO96/39851 (see, for example, examples 1-6). Suitable pianosounds include described in WO97/22257 (see, for example, on page 1, line 28, to page 2, line 19; page 4, line 13, to page 5, line 14; and page 10, line 35, to page 11, line 24).

In some preferred embodiments, the implementation of the cleaning compositions of the present invention include from about 0,0001% to about 10%, preferably from about 0,001% to about 0.2%, more preferably from about 0,005% to 0.1% pure enzyme carbohydrate oxidase in relation to the weight of the entire composition.

Additional enzymes that find use in the present invention include galactosidase (Novozymes A/S), cellobioside (Fermco Laboratories, Inc.), galactosidase (Sigma), pianosounds (Takara Shuzo Co.), carbothioamides (ICN Pharmaceuticals, Inc.) and oxidase (Genencor International, Inc.).

In other embodiments, to compositions of the type substrates, Lucaya such compounds, as sugar, glucose and/or galactose, in order to further increase the efficiency of the enzymatic bleaching.

Additional components of the cleaning composition

Additional components are used in the cleaning compositions of the present invention. Although it is not expected that the cleaning compositions of the present invention is limited thus, the various components described in more detail below. In fact, although such components are not significant for the purposes of the present invention, non-limiting list of supplements below, suitable for use in the present cleaning compositions and may be desirable for inclusion in certain embodiments of the present invention, for example to assist or enhance cleaning performance, for treatment of the cleaned substrate, or to modify the aesthetics of the cleaning composition, as is the case with perfumes, pigments, dyes or the like. It should be understood that such additives are in addition to the enzymes of the present invention, hydrogen peroxide and/or a source of hydrogen peroxide and material containing ester group. The exact nature of these additional components, and the extent of their inclusion will depend on the physical form of the composition and the type of cleaning process, in which oterom they should be applied. Suitable materials additives include, without limitation, surfactants, additives for improving the washing steps, chelating agents, inhibiting migration of the dye means, precipitating additives, dispersants, additional enzymes, and enzyme stabilizers, catalytic agents, bleaching activators, enhancers bleaching, pre-formed percolate, polymeric dispersing tools, clay tools to remove stains/to prevent re-deposition, Britney, defoamers, dyes, fragrances, means for imparting elasticity to the structure, fabric softeners, carriers, hydrotropes, processing AIDS and/or pigments. In addition to the following description, suitable examples of such other additives and the degree of application are found in U.S. patents No. 5576282, 6306812 and 6326348 included in the present description by reference. The above additional ingredients can form the rest of the cleaning compositions of the present invention.

SurfactantsIn some embodiments, the implementation of the cleaning composition proposed by the present invention include at least one surfactant and/or surfactant, where the surfactant is preferably selected from einnig surfactants, anionic surfactants, cationic surfactants, impolitically surfactants, zwitter-ionic surfactants, semipolar nonionic surfactants and mixtures thereof.

Surfactant is typically present in amount from about 0.1% to about 60%, from about 1% to about 50% or even from about 5% to about 40% by weight in consideration of the cleaning composition.

Cationic surfactants and salts of fatty acids with long-chainIn some embodiments, implementation of the present invention find application such cationic surfactants and salts of fatty acids with long chain, including salts of saturated or fatty acids, Elgiloy or alkenilovyh simple ester salts of carboxylic acids, salts or esters of a-califoirnia acids, surfactants amino acid type surfactant is a complex phosphate esters, Quaternary ammonium salts, including having from 3 to 4 alkyl substituents and up to 1 phenylseleno alkyl substituent. Suitable cationic surfactants and salts of fatty acids with long chain include described in British patent application No. 2094826 A description on which is expected in the present description by reference. In some embodiments, the implementation of the compositions include from about 1 to about 20 percent by weight of such cationic surfactants and salts of fatty acids with long chain.

Additives to enhance the cleansing actionIn some embodiments, implementation of the present invention compositions include from about 0 to about 10 percent by weight of one or more additives to enhance the cleaning action selected from the group consisting of alkali metal salts and alkanolamine salts of the following compounds: phosphates, phosphonates, phosphonocarboxylates, salts of amino acids, aminopolyamide high-molecular electrolytes, medicationbuy polymers, salts of dicarboxylic acids and aluminosilicate salts. Examples of suitable divalent linking agents described in British patent application No. 2094826 A, the description of which is incorporated into this description by reference.

In additional embodiments, the implementation of the compositions of the present invention contain from about 0 to about 10 percent by weight of one or more alkali metal salts of the following compounds as alkali or inorganic electrolytes: silicates, carbonates and sulfates, and organic bases such as triethanolamine, diethanolamine, monoethanol is in and triisopropanolamine. In some embodiments, the implementation of the cleaning compositions of the present invention contain one or more additives to enhance the cleaning action of the detergent and/or additives systems to improve the washing steps. When additives are used to improve the washing steps, the target cleaning composition typically contains relatively low amounts (for example, from about 0% to about 10% additive to enhance the cleansing action of the weight of the considered cleaning composition).

In various embodiments, the implementation of additives to improve the washing steps include, without limitation, alkali metals, ammonium and alkanolammonium salts of polyphosphates, alkali metal silicates, carbonates of alkali and alkaline earth metals, aluminosilicate additives to improve the washing steps, polycarboxylate compounds, ether hydroxypolycarboxylic, copolymers of maleic anhydride with ethylene or vinylmations ether, 1,3,5-trihydroxybenzene-2,4,6-trisulfonic acid and carboxymethylcysteine acid, the various alkali metal, ammonium and substituted ammonium salts polixeni acids, such as ethylenediaminetetraacetic acid, nitrilotriacetic acid, and polycarboxylate, such as malletova acid, succinic acid, citric acid, accidentally Isleta, primulina acid, benzene-1,3,5-tricarboxylic acid, carboxymethylcysteine acid and its soluble salts.

Chelating agentsin some embodiments, the implementation of the cleaning composition proposed by the present invention contain at least one chelating agent. Suitable chelating agents include copper, iron and/or magnesium chelating agents, and mixtures thereof.

In some preferred embodiments, the implementation of which include at least one chelating agent, the cleaning compositions comprise from about 0.1% to about 15% or from about 0.5% to about 5% of at least one chelating agent by weight of the considered cleaning composition.

Precipitating additivesIn some other embodiments, the implementation of the cleaning composition proposed by the present invention contain precipitating additive. Suitable precipitating additives include polyethylene glycol, polypropyleneglycol, polycarboxylate, soil release polymers, such as polyterephthalic acid, clays, such as kaolinite, montmorillonite, atapulgite, illite, bentonite, halloysite, and mixtures thereof.

Means to prevent re-depositionIn other additional embodiments, the implementation of the present invention the compositions contain from p is blithedale 0.1 to about 5 percent by weight of one or more of the following compounds as a means to prevent re-deposition of: polyethylene glycol, polyvinly alcohol, polyvinylpyrrolidone and carboxymethylcellulose. In some preferred embodiments, the implementation used a combination of carboxymethylcellulose and/or polyethylene glycol with a composition of the present invention as compositions suitable for removing dirt.

Inhibiting the transfer of dye fundsIn other embodiments, the implementation of the cleaning compositions of the present invention include one or more means of inhibiting the migration of the dye. Suitable polymeric inhibiting migration of the dye tools include, without limitation, polyvinylpyrrolidone polymers, polymers, polyamine-N-oxide, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polivinilatsetatny and polyvinylimidazole or mixtures thereof.

When they are present in the present cleaning compositions, inhibiting migration of the dye funds are usually present in amounts of from about 0,0001% to about 10%, from about 0.01% to about 5% or from about 0.1% to about 3% by weight of the cleaning composition.

The dispersantIn additional embodiments, the implementation of the cleaning compositions of the present invention contain a dispersant. Suitable water-soluble organic materials include Homo - or copolymer acid or their salts, in which polycarbon the Wai acid contains at least two carboxyl radical, separated from each other by no more than two carbon atoms.

EnzymesIn other embodiments, the implementation of the cleaning composition proposed by the present invention optionally comprise one or more detergent enzymes which provide cleaning performance and/or win in fabric care. Examples of suitable enzymes include, without limitation, hemicellulase, peroxidase, protease, metalloprotease, cellulase, xylanase, lipase, phospholipase, esterase, cutinase, pectinase, keratinase, reductase, oxidase, peroxidase, lipoxygenase, ligninase, pullulanase, tannaz, pentosanase, Malagasy, mannanase, cellulase, β-glucanase, arabinosidases, hyaluronidase, chondroitinase, laccase, and amylases, or mixtures thereof. In some embodiments, the implementation of the combination is the usual cocktail of suitable enzymes (e.g. proteases (proteases), lipase (lipase), cutinase (cutinase) and/or cellulase (cellulases), used in conjunction with amylase (amylase)).

Stabilizers enzymes- Enzymes for use in detergents can be stabilized by using different methods. In some embodiments, implementation of the present invention the enzymes are stabilized by the presence of water-soluble sources of calcium ions and/or magnesium in the finished compositions is s, which provide enzymes such ions.

The catalytic metal complexes In other embodiments, the implementation of the cleaning compositions of the present invention include at least one catalytic metal complex. In some embodiments, the implementation of the present invention find application metal-containing catalysts whitening containing catalyst system containing a transition metal cation with a particular whitening catalytic activity, such as the cations of copper, iron, titanium, ruthenium, tungsten, molybdenum, or magnesium, an auxiliary metal cation having a low or missing whitening catalytic activity, such as the cations of zinc or aluminum, and komleksoobrazovateli having defined stability constants with respect to the catalytic and auxiliary metal cations, particularly ethylenediaminetetraacetic acid, ethylenediaminetetra(methylenephosphonate acid) and its soluble salts (see, for example, U.S. patent No. 4430243, hereby incorporated by reference in its entirety).

In some embodiments, the implementation of the compositions of the present invention catalyze by compounds of magnesium. Such compounds and used the number of well-known in the art and include the (see, for example, catalysts based on magnesium, described in U.S. patent 5576282, hereby incorporated by reference in its entirety).

Cobalt catalysts bleaching also find use in the present invention. Such compositions are known in the art (see, for example, US 5597936 and US 5595967). Such cobalt catalysts can be easily obtained by known methods (see, for example, U.S. 5597936 and U.S. 5595967).

In some embodiments, the implementation of the compositions of the present invention include at least one complex of a transition metal macropolitical rigid ligand ("MRL"). In practice, and not with the purpose of limitation, the compositions and cleaning methods of the present invention can be adjusted in such a way as to ensure order at least one active molecule MRL on a hundred million in water for washing, and usually preferably provide from about 0,005 ppm to about 25 ppm, more preferably from about 0.05 ppm to about 10 ppm and most preferably from about 0.1 ppm to about 5 ppm MRL in the washing solution.

In some embodiments, implementation of the preferred transition metals are considering a transition-metal catalyst bleaching include magnesium, iron and chromium. In some other variations of the preferred implementation MRL, used in the present invention, represent a special type of super-rigid ligands with cross-links, such as 5,12-diethyl-1,5,8,12-tetraazabicyclo[6.6.2]hexadecane.

Suitable transition metal MRLS can be easily obtained by known methods (see, for example, WO 00/332601 and U.S. 6225464; both are included in the present description by reference in its entirety).

BleachIn some embodiments, the implementation of the present invention provides that the use of perhydrol of the present invention in combination with an additional bleaching agent (means), such as percarbonate sodium, perborate sodium, sodium sulfate/adduct of hydrogen peroxide and sodium chloride/adduct of hydrogen peroxide and/or photosensitive bleaching dye, such as zinc or aluminum salt from sulphonated phthalocyanine will improve detergency. In additional embodiments, the implementation of perhydrol of the present invention used in combination with amplifiers bleaching (for example, TAED and/or NOBS).

Podsinwowa tools and fluorescent dyesIn some embodiments, implementation of the present invention in the composition is injected podsinwowa tools and fluorescent dyes. Examples of suitable podsinwowa funds and fluorescent dyes described in the British patent application No. 2094826 A, the description of which is incorporated into this description by reference.

Anti-caking agentsIn some embodiments, implementation of the present invention, in which the composition is a powder or a solid, the composition is injected anti-caking agents. Examples of suitable anti-caking agents include salts of p-toluensulfonate acids, salts cellsurvival acid, salts of acetic acid, salt sulfonterol acid, talc, finely pulverized silica, clay, calcium silicate (for example, Micro-Cell [Johns Manville Co.]), calcium carbonate and magnesium oxide.

AntioxidantsIn some additional embodiments, the implementation in the composition of the present invention include at least one antioxidant. In some particularly preferred embodiments, the implementation of the antioxidants include, for example, tert-butyl-hydroxytoluene, 4,4'-butylidene-bis-(6-tert-butyl-3-METHYLPHENOL), 2,2'-butylidene-bis-(6-tert-butyl-4-METHYLPHENOL), modified monasterolo the cresol-modified destirol the cresol-modified monasterolo phenol-modified destirol phenol and 1,1-bis-(4-hydroxyphenyl)cyclohexane.

SolventsIn some embodiments, the implementation of the compositions of the present invention also include solvents, including, without limitation, lower alcohols (e.g. ethanol benzosulfimide salt and substituted lower alkilani benzosulfimide salt, such as p-toluensulfonate salt), glycols such as propylene glycol, acetylbenzenesulfonyl salt, acetamide, amides pyridinedicarboxylic acid, benzoate salt and urea.

In some embodiments, the implementation of the detergent compositions of the present invention is used in shirobom pH range from acidic to alkaline pH. In some preferred embodiments, the implementation of the detergent composition of the present invention is used in slightly acid, neutral or alkaline scrubbing medium having a pH above about 4 to not more than about 11.

In addition to the ingredients described above, fragrances, buffers, preservatives, dyes and the like are also used with the present invention. These components are available in concentrations and forms known to specialists in this field of technology.

In some embodiments, the implementation of the powdered detergent basis of the present invention receive any known means of obtaining (for example, methods of spray drying and granulation methods). In some preferred embodiments, the implementation of the detergents used bases obtained using the method of spray drying and/or method (methods) granulation by spray drying. Detergent base, obtained using the method of spray drying is not regulated by relating the structure to the conditions obtaining. In some preferred embodiments of implementing the method of drying by atomization get hollow granules obtained by spraying a water suspension of heat-resistant ingredients such as surface active agents and additives to enhance the cleansing action in space with a high fever. In some embodiments, the implementation after drying, spray add fragrances, enzymes, bleaching agents, inorganic alkaline additives to improve the washing steps. In some embodiments, implementation, applying a highly concentrated granular detergent base, obtained by means of granulation by spray drying, after getting the basics also add different ingredients.

In some embodiments, implementation, use liquid detergent base, the base is a homogeneous solution, while in other embodiments, the implementation of it is a non-homogeneous variance.

In some embodiments, the implementation of the detergent compositions of the present invention is incubated with the tissue (for example, soiled tissues), in industrial and home applications at temperatures, reaction times and the modules bath is usually used in these conditions. The incubation conditions (i.e. conditions that are effective for the treatment of materials detergent compositions in compliance and with the present invention) can be easily identified by experts in the field of technology. Accordingly, suitable conditions effective for the treatment of these detergents, fit those with similar detergent compositions which include peligrosas wild-type.

As indicated above, in some embodiments, the implementation of the detergents, proposed by the present invention, used as zamachivalas in the appropriate solution at intermediate pH, where there is sufficient activity in order to deliver the desired improvements, such as mitigation, clean-up roll, warning, roll-off, removing the fibers from the surface and/or cleaning. When washing composition is a composition for soaking (for example, pre-washing or pre-processing), in the form of liquid, aerosol, gel or paste-like compositions, the enzyme peligrosas usually used from about 0.00001% to about 5% by weight, in regard to the total weight of the composition for soaking or pre-treatment. In some embodiments, the implementation of such compositions do not necessarily apply at least one surface-active substance. If they are used, such surfactants are usually present in a concentration of from about 0.0005 to about 1 percent by weight, relative to the total weight of soaked. OST is supplemented flax part of the composition contains conventional components, used for soaking (e.g., diluents, buffers, other enzymes (e.g. protease), and so on), their concentrations.

The cleaning composition

The cleaning compositions of the present invention find use in various applications, including use for washing, cleaning hard surfaces, application for automatic dishwashing, as well as for cosmetic applications, such as cleaning dentures, teeth, hair and/or skin. However, due to the unique advantages of increased effectiveness in lower temperature solutions and excellent profile, color preserving enzymes of the present invention are ideally suited for applications for washing, such as the bleaching of fabrics. Moreover, the enzymes of the present invention find use in granular and liquid compositions.

The enzymes of the present invention also find application in the extension of the cleaning products. Incremental cleaning products, including enzymes of the present invention are ideally suited for inclusion in the processes of washing, which is desirable for more efficient whitening. Such cases include, without limitation, applications for low-temperature cleaning solution. In some embodiments, the implementation of the extension product is in its simplest form, the Dean or several enzymes of the present invention. In some embodiments, the implementation of additives (additives) are packaged in a unit dosage form suitable for addition to a cleaning process in which is used a source of active oxygen, and the desired high efficiency of bleaching. In some embodiments, the implementation form of a single dose is a bean, while in other embodiments, the implementation of it is a tablet, gelatin capsule or other unit with a single dose, such as pre-measured powders or liquids. In some preferred embodiments of include at least one excipient or carrier, in order to increase the amount of such a composition. Suitable materials, fillers or carriers include, without limitation, various salts of sulfates, carbonates and silicates, as well as talc, clay and the like. In some embodiments, implementation materials, fillers or carriers for liquid compositions include water or primary and secondary alcohols of low molecular weight, including polyols and diols. Examples of such alcohols include, without limitation, methanol, ethanol, propanol and isopropanol. In some embodiments, the implementation of the compositions include from about 5% to about 90% of these materials. In some embodiments, the implementation of the acid fillers are the label in a decrease in pH. In some alternative embodiments, the implementation of a cleaning additive includes at least one source of active oxygen and/or additional ingredients as described in the present description.

Cleaning compositions and cleaning additives of the present invention require effective amount of enzymes that are offered by the present invention. In some particularly preferred embodiments, the necessary amount of enzyme reach by adding one or more kinds of perhydrol M. smegmatis, variants, homologues and/or other enzymes or fragments of enzymes having the activity of enzymes of the present invention. Typically, the cleaning compositions of the present invention contain at least 0.0001 percent by weight, from about 0.0001 to about 1, from about 0.001 to about 0.5, or even from about 0.01 to about 0.1 percent by weight of at least one enzyme of the present invention.

In some embodiments, the implementation of the cleaning compositions of the present invention include a material selected from the group consisting of a source of active oxygen, hydrogen peroxide and mixtures thereof, and a source of active oxygen selected from the group consisting of:

(i) from about 0.01 to about 50, from about 0.1 to PR is approximately 20, or even from about 1 to 10 percent by weight of percale, organic peroxyacids, of hydrogen peroxide with urea and mixtures thereof;

(ii) from about 0.01 to about 50, from about 0.1 to about 20, or even from about 1 to 10 percent by weight of carbon and from about 0.0001 to about 1, from about 0.001 to about 0.5, from about 0.01 to about 0.1 percent by weight of the carbohydrate oxidase; and

(iii) mixtures thereof.

Suitable parsoli include selected from the group consisting of alkali metal perborate, percarbonate alkali metals, perphosphate alkali metals, persulfates alkali metals and mixtures thereof.

In some preferred embodiments, the implementation of the carbohydrate(s) selected from the group consisting of monoglide, diplomado, triplewides, oligoplites and mixtures thereof. Suitable carbohydrates include carbohydrates selected from the group consisting of D-arabinose, L-arabinose, D-cellobiose, 2-deoxy-D-galactose, 2-deoxy-D-ribose, D-fructose, L-fucose, D-galactose, D-glucose, D-glycero-D-Gulo-heptose, D-lactose, D-lyxose, L-lyxose, D-maltose, D-mannose, melezitose, L-melibiose, palatinose, D-raffinose, L-ramnose, D-ribose, L-sorbose, stachyose, sucrose, D-trehalose, D-xylose, L-xylose, and mixtures thereof.

Suitable carbohydrate oxidase include carbohydrate oxidase selected from the group consisting of alsoeasy (IUPAC classification EC 1.1.3.9), galactosidase (IUPAC classification EC l.1.3.9), cellobioside (IUPAC classification EC l.1.3.25), pianosounds (IUPAC classification EC l.1.3.10), carbothioamides (IUPAC classification EC l.1.3.11) and/or exotoxicity (IUPAC classification EC l.1.3.5), glucose oxidase (IUPAC classification EC 1.1.3.4) and mixtures thereof.

In some preferred embodiments, the implementation of the cleaning compositions of the present invention also contain from about 0.01 to about 99,9, from about 0.01 to about 50, from about 0.1 to 20, or even from about 1 to about 15 percent by weight molecules containing ester group. Suitable molecules containing ester group can have the formula:

R1Ox[(R2)m(R3)n]p

in which R1represents a group selected from the group consisting of H or substituted or unsubstituted alkyl, heteroalkyl, alkenyl, quinil, aryl, alkylaryl, alkylglycerol and heteroaryl; in one aspect of the present invention R1may contain from 1 to 50,000 carbon atoms, from 1 to 10,000 carbon atoms, or even from 2 to 100 carbon atoms;

each R2represents alkoxylate group, in one aspect of the present invention each R2PR is dstanley an independent ethoxylate, propoxylate or butoxylate group;

R3represents a group forming an ester, with some of the options for implementation with the formula:

R4CO-, where R4represents H, substituted or unsubstituted alkyl, alkenyl, quinil, aryl, alkylaryl, alkylglycerol and heteroaryl, in one aspect of the present invention R4represents a substituted or unsubstituted alkyl, alkenyl, quinil, group containing from 1 to 22 carbon atoms, aryl, alcylaryl, alkylglycerols or heteroaryl group containing from 4 to 22 carbon atoms, or R4represents a substituted or unsubstituted C1-C22alkyl group, or R4represents a substituted or unsubstituted C1-C12alkyl group; x represents 1 when R1represents H; when R1is not H, x is an integer that is equal to or less than the number of carbon atoms in R1;

p is an integer that is equal to or less than x;

m is an integer from 0 to 50, an integer from 0 to 18 or an integer from 0 to 12, and n is at least 1.

In one aspect of the present invention, the molecule containing the ester group is alkylalkoxy or propoxylate, the ima is the overall formula R 1Ox[(R2)m(R3)n]pwhere:

R1represents a C2-C32substituted or unsubstituted alkyl or heteroalkyl group;

each R2represents independently ethoxylate or propoxylate group;

R3represents a group forming an ester having the formula:

R4CO-, where R4represents H, substituted or unsubstituted alkyl, alkenyl, quinil, aryl, alkylaryl, alkylglycerol and heteroaryl, in one aspect of the present invention R4represents a substituted or unsubstituted alkyl, alkenylphenol or alkenylphenol group containing from 1 to 22 carbon atoms, a substituted or unsubstituted aryl, alcylaryl, alkylglycerols or heteroaryl group containing from 4 to 22 carbon atoms, or R4represents a substituted or unsubstituted C1-C22alkyl group, or R4represents a substituted or unsubstituted C1-C12alkyl group;

x is an integer that is equal to or less than the number of carbon atoms in R1;

p is an integer that is equal to or less than x;

m is an integer from 1 to 12 and

n is at least 1.

In one aspect of this image is etenia molecule, containing ester group has the formula:

R1Ox[(R2)m(R3)n]p

where R1represents H or a group containing a primary, secondary, tertiary or Quaternary amino group, and the group R1that contains the amino group, which are selected from the group consisting of substituted or unsubstituted alkyl, heteroalkyl, alkenyl, quinil, aryl, alkylaryl, alkylglycerol and heteroaryl; in one aspect of the invention applicants R1contains from 1 to 50,000 carbon atoms, from 1 to 10,000 carbon atoms, or even from 2 to 100 carbon atoms;

each R2represents alkoxylate group, in one aspect of the present invention each R2represents independently ethoxylate, propoxylate or butoxylate group;

R3represents a group forming an ester having the formula:

R4CO-, where R4can represent H, substituted or unsubstituted alkyl, alkenyl, quinil, aryl, alkylaryl, alkylglycerol and heteroaryl, in one aspect of the present invention R4may be a substituted or unsubstituted alkyl, alkenylphenol or alkenylphenol group containing from 1 to 22 carbon atoms, a substituted or unsubstituted aryl, alcylaryl, alkylglycerols or GE is erourolliou group, containing from 4 to 22 carbon atoms, or R4may be a substituted or unsubstituted C1-C22alkyl group, or R4may be a substituted or unsubstituted C1-C12alkyl group;

x represents 1 when R1represents H; when R1is not H, x is an integer that is equal to or less than the number of carbon atoms in R1;

p is an integer that is equal to or less than x;

m is an integer from 0 to 12, or even from 1 to 12; and

n is at least 1.

In some embodiments, implementation of any of the above aspects of the present invention, the molecule containing the ester group has an average molecular weight less than approximately 600,000 daltons, less than approximately 300,000 daltons, less than approximately 100,000 daltons, or less than about 60,000 daltons.

Suitable molecules containing ester group include Poliplast, which contain the ester group.

The cleaning composition proposed by the present invention are usually such that during the application processes for water purification washing water has a pH from about 5.0 to about 11,5, or even from priblizitelen is 7.5 to about 10.5. the Liquid formulations of the product are usually so that the pH was between approximately 3.0 and approximately 9,0. Granular products for washing are usually so that the pH ranged from about 9 to about 11. Technologies for controlling pH at recommended level include the use of buffers, alkalis, acids, etc. and are well known to the person skilled in the technical field.

In some embodiments, implementation, when the enzyme(s) of the present invention applied in the form of a granular composition or liquid, for the enzyme (enzymes) preferably take the form of coated particles, in order to protect this enzyme from other components of the granular or liquid compositions during storage. In addition, the conclusion in the shell provides a means of monitoring the suitability of the enzyme (enzymes) during the cleaning process. In some embodiments, the implementation of the conclusion in the shell increases the efficiency of the enzyme (enzymes). When this enzyme(s) wrap the using any suitable material for the shell, known in this technical field.

In the sheath material usually make at least part of the enzyme (enzymes). Typically, the shell material is water-soluble and/or dispersible in water. In some embodiments, the implementation of the sheath material to them is no glass transition temperature (Tg) of 0°C or above (see, for example, WO 97/11151, especially page 6, line 25 to page 7, line 2, for details about the glass transition temperatures).

In some embodiments, the implementation of the sheath material selected from the group consisting of carbohydrates, natural resins, synthetic resins, chitin, chitosan, cellulose, cellulose derivatives, silicates, phosphates, borates, polyvinyl alcohol, polyethylene glycol, paraffin waxes, and combinations thereof. When the membrane material is a carbohydrate, it is usually selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, and combinations thereof. In some preferred embodiments, the implementation of the shell material is starch (see, for example, European patent 0 922 499, U.S. patent No. 4977252, U.S. patent No. 5354559 and U.S. patent No. 5935826 to describe some suitable starches).

In some alternative embodiments, the implementation of the sheath material is a microsphere made from plastic material (materials), including, without limitation, thermoplastics, Acrylonitrile, Methacrylonitrile, polyacrylonitrile, polymethacrylates and mixtures thereof. Suitable commercially available microspheres include EXPANCEL® (Expancel, Stockviksverken, Sweden), PM 6545, PM 6550, PM 7220, PM 7228, EXTENDOSPHERES®, LUXSIL®, Q-CEL® and SPHERICEL® (PQ Corp., Valley Forge, PA).

Methods of making and applying the cleaning composition is th present invention

The cleaning compositions of the present invention be in any suitable form and produced by any of the developer's method (see, for example, U.S. patents№5879584; 5691297; 5574005; 5569645; 5565422; 5516448; 5489392; and 5486303; they are all included in the present description by reference to non-limiting examples).

Method of use

The cleaning compositions described in the present description, are used in the cleaning of fabrics and/or surfaces. Normally at least a portion of the section to be cleaned is brought into contact with an embodiment of the present cleaning compositions, in pure form or dissolved in the washing solution, and then the specified site does not necessarily washed and/or rinsed. For the purposes of the present invention wash includes, without limitation, cleaning brush and mechanical stirring. The fabric includes any suitable fabric, suitable for washing in normal use by the consumer conditions. The cleaning compositions of the present invention is usually used in concentrations of from about 500 ppm to about 15000 ppm in solution. When the cleaning solution is a water, the water temperature typically lies in the range from approximately 5°to approximately 90°C. In the variants of implementation, which clean the fabric, the mass ratio of water to fabric is usually from priblizitel is but 1:1 to about 30:1.

EXPERIMENTAL PART

The following examples are offered to demonstrate and more to illustrate certain preferred embodiments of aspects of the present invention, and should not be taken as limiting its scope.

In the experimental description that follows, use the following abbreviations: °C (degrees centigrade); rpm (revolutions per minute); H2O (water); HCl (hydrochloric acid); aa (amino acid); bp (base pair); kb (thousands of base pairs); kDa (kilodalton); g (grams); μg (micrograms); mg (milligrams); ng (nanogram); μl (Microlitre); ml (milliliters); mm (millimeters); nm (nanometers); μm (micrometer); M (molar); mm (millimolar); μm (micromolar); U (units); (volts); MW (molecular weight); sec (seconds); min (minutes); h (hours); MgCl2(magnesium chloride); NaCl (sodium chloride); OD280(optical density at 280 nm); OD600(optical density at 600 nm); page (polyacrylamide gel electrophoresis); GC (gas chromatography); EtOH (ethanol); FSB (phosphate-salt buffer [150 mm NaCl, 10 mm sodium phosphate buffer, pH 7,2]); LTOs (sodium dodecyl sulphate); Tris (Tris(hydroxymethyl)aminomethane); TAED (N,N,N, N'-tetraacetylethylenediamine); weight/about (weight to volume); V/V (volume to volume); Per (perhydrol); per (gene peligrosas); Ms (M. smegmatis); MS (mass spectroscopy); BRAIN (BRAI Biotechnology Research and Information Network, AG, Zwingenberg, Germany); TIGR (The Institute for Genomic Research, Rockville, MD); AATCC (American Association of Textile and Coloring Chemists); WFK (wfk Testgewebe GmbH, Bruggen-Bracht, Germany); Amersham (Amersham Life Science, Inc. Arlington Heights, EL); ICN (ICN Pharmaceuticals, Inc., Costa Mesa, CA); Pierce (Pierce Biotechnology, Rockford, IL); Amicon (Amicon, Inc., Beverly, MA); ATCC (American Type Culture Collection, Manassas, VA); Amersham (Amersham Biosciences, Inc., Piscataway, NJ); Becton Dickinson (Becton Dickinson Labware, Lincoln Park, NJ); BioRad (BioRad, Richmond, CA); Clontech (CLONTECH Laboratories, Palo Alto, CA); Difco (Difco Laboratories, Detroit, MI); GIBCO BRL or Gibco BRL (Life Technologies, Inc., Gaithersburg, MD); Novagen (Novagen, Inc., Madison, WI); Qiagen (Qiagen, Inc., Valencia, CA); Invitrogen (Invitrogen Corp., Carlsbad, CA); Genaissance (Genaissance Pharmaceuticals, Inc., New Haven, CT); DNA 2.0 (DNA 2.0, Menlo Park, CA); MIDI (MIDI Labs, Newark, DE) InvivoGen (InvivoGen, San Diego, CA); Sigma (Sigma Chemical Co., St. Louis, MO); Sorvall (Sorvall Instruments, a subsidiary of DuPont Co., Biotechnology Systems, Wilmington, DE); Stratagene (Stratagene Cloning Systems, La Jolla, CA); Roche (Hoffmann La Roche, Inc., Nutley, NJ); Agilent (Agilent Technologies, Palo Alto, CA); Minolta (Konica Minolta, Ramsey, NJ); and Zeiss (Carl Zeiss, Inc., Thornwood, NY); Genencor (Genencor International, Inc. Palo Alto, CA); Expancel (Expancel, Stockviksverken, Sweden); PQ Corp. (PQ Corp., Valley Forge, PA); BASF (BASF Aktiengesellschaft, Florham Park, NJ); Monsanto (Monsanto Co., St. Louis, MO); Novozymes (Novozymes A/S, Bagsvaerd, Denmark); Wintershall (Winterschall AG., Kassel, Germany); Gist-Brocades (Gist-Brocades, NV, Ma Delfit, The Netherlands); Enichem (EniChem Americas, Inc., Houston, TX); Huntsman (Huntsman Corp., Salt Lake City, UT); Fluka (Fluka Chemie AG, Buchs, Switzerland); and Dow Corning (Dow Corning Corp., Midland, MI).

Additional abbreviations used for detergent compositions, presented in the following table:

LAS: Linear C11-13Las sodium
TAS:Solid alkylsulfate sodium
CxyAS:C1x-C1yalkylsulfate sodium
CxyEz:C1x-C1ypredominantly linear primary alcohol condensed with an average of z moles of ethylene oxide
CxyAEzS:C1x-C1yalkylsulfate sodium, condensed with an average of z moles of ethylene oxide (added the names of the molecules shown in the examples)
Nonionic:mixed ethoxylated/propoxycarbonyl fatty alcohol (e.g., Plurafac LF404), in particular alcohols with an average degree of amoxilonline of 3.8 and an average degree of propoxycarbonyl 4,5
QAS:R2N+(CH3)2(C2H4OH) with R2= C12-C14
Silicate:Amorphous sodium silicate (ratio of SiO2:Na2O = 1,6-3,2:)
Metasilicate:Metasilicate sodium (ratio SiO2:Na2O = 1,0)
Zeolite And:Hydrogenated aluminosilicate formula Na12(AlO2SiO2)12·27H2O
SKS-6:Crystalline layered silicate of formula δ-Na2Si2O5
Sulfate:Anhydrous sodium sulfate
STPP:Sodium tripolyphosphate
MA/AA:A random copolymer of acrylate/maleate 4:1, approximately 70000-80000
AA:Polymer sodium polyacrylate average molecular weight 4500
Policer-boxylic:The copolymer containing a mixture of carboxylate monomers such as acrylate, maleate and methacrylat with MV in the range 2000-80000 (for example, Sokolan™, a copolymer of acrylic acid, MV 4500; BASF)
BB1:3-(3,4-dihydroisoquinoline)propanesulfonate
BB2:1-(3,4-dihydroisoquinoline)decane-2-sulfate
PB1:Perborate sodium monohydrate
PB4:Perborate sodium tetrahydrate of nominal formula NaBO3·4H2O
Percarbonate:Percarbonate sodium nominal formula 2Na2CO3·3H2O2
TAED:Tetraacetylethylenediamine

NOBS:Nonanoyloxybenzenesulfonate in the form of sodium salt
DTPA:Diethylenetriaminepentaacetic acid
HEDP:1,1-hydroxyethylphosphonate acid
DETPMP:Diet is streaminit(methylene)phosphonate (e.g., Dequest 2060™; Monsanto)
EDDS:The Ethylenediamine-N,N'-diantara acid, (S, S) isomer in the form of its sodium salt,
Diamine:Dimethylaminopropylamine; 1,6-hexanediamine; 1,3-propandiamine; 2-methyl-1,5-pentanediamine; 1,3-pentanediamine; 1-methyl-diaminopropan
DETBCHD:5,12-diethyl-1,5,8,12-tetraazabicyclo[6,6,2]hexadecane, dichloride salt of Mn(II)
PAAC:Salt of cobalt(III) pentamycetin
Paraffin:Paraffin oil (such as Winog 70™; Wintershall)
Paraffin-
sulfonate
:Parafine oil or wax, in which some hydrogen atoms are substituted by sulphonate groups
Aldose-
oxidase
:The enzyme oxidase (e.g., aldose oxidase; Novozymes)
Galactose oxidase:Galactosidase (for example, from Sigma)
Protease:Proteolytic enzymes (e.g., SAVINASE, ALCALASE®, EVERLASE®; Novozymes; and "Protease A", described in US RE 34606 on Figa, 1B and 7, and column 11, lines 11-37; "Protease B", described in US 5955340 and US57005676 on Figa, 1B and 5 and in table 1; and "Protease C", described in US 63125936 and US 6482628 figure 1-3 [SEQ ID 3] and in column 25, line 12, and "Protease D" is option 101G/103A/104I/159D/232V/236H/245R/248D/252K (BPN ' numbering'), described in WO 99/20723, and ASP, described in US04/039006; Genencor)
Amylase:Amylolytic enzymes (e.g., PURAFECT® Ox Am; described in WO 94/18314, WO 96/05295 in Genencor; and NATALASE®, TERMAMYL®, FUNGAMYL® and DURAMYL®; Novozymes)
Lipase:Lipolytica enzymes (e.g., LIPOLASE®, LBPOLASE® Ultra; Novozymes; and Lipomax™; Gist-Brocades)
Cellulase:Cellulities enzymes (e.g., CAREZYME®, CELLUZYME®, ENDOLASE®; Novozymes)
Pectinase:PECTAW AY® and PECTA WASH® (Novozymes).
PVP:Polyvinylpyrrolidone with an average molecular weight of 60000
PVNO: Polyvinylpyridine-N-oxide with an average molecular weight of 50000
PVPVI:A copolymer of vinylimidazole and vinylpyrrolidone, with an average molecular weight of 20000
Brightener 1:Disodium 4,4'-bis(2-colfosceril)biphenyl -
Silicone antispin-
Vatel
:Polydimethylsiloxanes regulator foam with a copolymer of siloxane-oxyalkylene as a dispersing means with respect to the regulator foam to the dispersing medium from 10:1 to 100:1
Antifoam:12% silicone/silica, 18% stearic alcohol, 70% starch in granular form
SRP 1:Polyesters with terminal anionic groups
PEG X:Polyethylene glycol with molecular weight "X"
PVP K60 ®:Vinylpyrrolidone the homopolymer (average MV 160000)
Jeffamine ® ED-2001 :Blocked polyethylene glycol (for example, from Huntsman)
Isachem ® AS:Branched alcohol alkylsulfate (for example, from Enichem)
MME PEG (2000):Onomatology ether of polyethylene glycol (MW 2000) (for example, from Fluka).
DC3225C:Silicone defoamer, a mixture of silicone oil and silica (for example, from Dow Corning).
TEPAE:Tetraethylenepentaamine
BTA:Benzotriazol
Betaine:(CH3)3N+CH2COO-
Sugar:D-glucose production purity or sugar food purity
CFAA:C12-C14alkyl-N-methylglucamide
TPKFA:All fraction C12-C14 fatty acids, limited downwards on the chain length
Clay:Hydrogenated aluminosilicate General formula
Al2O3SiO2·xH2O (for example, kaolinite, montmorillonite, atapulgite, illite, bentonite, halloysite).
MCAEM:Esters of the formula R1Ox[(R2)m(R3)n]p
the pH of the composition:Measured in a 1% solution in distilled water at 20°C
Peligrosas:The enzyme described in US 04/040438, including wild type (DT) and options (for example, S54V).
ZPB:The Quaternary ammonium compound hexamethylenediamine were-E24-dimethyl, tetrasulfate

In some of the following experiments to measure the optical density of the product obtained after completion of the reaction, used the spectrophotometer. For reflectivity measurements the samples used reflectometer. Unless otherwise stated, the concentration of protein was determined using Coomassie Plus (Pierce), using BSA as standard.

EXAMPLE 1

Analysis of enzymes

This example describes how to determine the purity and activity of the enzymes used in the following examples and throughout this specification.

Analysis of enzyme activity (pNB analysis)

This activity was measured by hydrolysis of p-nitrophenylacetate. The reaction mixture obtained by adding 10 μl of 100 mm p-nitrophenylacetate in dimethyl sulfoxide in 990 ml of 100 mm Tris-HCl buffer pH 8.0 containing 0.1% Triton X-100. The background rate of hydrolysis was measured before adding the enzyme at 410 nm. The reaction starts by adding 10 ál of enzyme to 990 ml of the reaction mixture and measuring the change in optical density at 410 nm at room temperature (~23°C). The results, adjusted for background record as δA410/min/ml or δA410/min/mg protein.

Weight enzyme components proposed in the present description, calculated from the total number of active protein. All interest amounts and the relationship is calculated by weight, unless otherwise indicated. All interest amounts and the relationship is calculated with respect to the entire composition, unless otherwise indicated.

EXAMPLE 2

The influence of pH on the efficiency of peracetic acid cleaning

This example describes experiments conducted to determine the effect of pH on affectionate Peru is ssnoi acid for cleaning.

A. Method of determining the cleaning efficiency in the small scale

Dyes:

Colorants for the study were obtained from commercial suppliers (i.e., Testfabrics). The target of the tested dyes represented consumer dirty t-shirts, consumer dirty pillowcases, ready stains from tea (Testfabrics, Tea for Low Temp on Cotton, STC CFT BC-3) and ready stains from wine (Testfabrics, Cotton Soiled with Wine, STC CFT CS-3). Consumer contaminated products were used as ballast to fill the machine boots to 0.6 pounds per 2 gallons. Consumer items were collected and prepared from the filthy garments donated by local residents. A solid area of staining was identified and cut into samples of approximately 4 inches by 4 inches from the target contaminated consumer the analyzed spots. These samples are then cut in half and marked for use in the comparison of the two water treatment options.

The Protocol of washing on a small scale:

Preparation:

Usually in experiments by washing in a small scale compared five different treatment options in three Parallels, using two copies of each target spot on treatment option. Therefore, in each treatment were used for two samples each prepared spot. Consumer the test spots were prepared thereby each treatment was subjected to a half of the sample, a steam room, each in the other treatments. Therefore, the test of the five treatment options covered 10 pairs of consumer testing of patches, where each treatment covered 4 half spots to provide a pairwise comparison. This pairwise ordering consumer test spots was done twice for all treatment options. Dyes were combined, weighed for each treatment and added consumer contaminated ballast in order to get a final load of 0.6 lbs. The treated composition was weighed or divided into aliquots, depending on the shape of the component. The solution of hard water with a ratio of calcium to magnesium 3:1, 10000 granov per gallon (gpg), obtained by dissolving 188,57 g of calcium chloride dihydrate and 86,92 g of magnesium chloride uranyl in 1 l of purified water.

The order of washing:

1. Five small drums of washing machines with a vertical load filled 2 gallon (7,75 l) of deionized water at 60°F.

2. The hardness of water is measured and brought up to 6 gpg by adding a solution of hard water.

3. Processed components were added in respective drums and started stirring at 75 rpm

4. Measured and regulated pH using 1N. NaOH or HCl to the desired level.

5. Stopped PE emiliania, added spots and burden, then once again began stirring.

6. Spots and products were washed for 12 minutes. Throughout washing was observed for pH.

7. Within 2 minutes, poured and pressed at an average speed of the washing machine.

8. Again filled drums 2 gallons deionizovannoy water at 60°F and regulate the hardness of the water.

9. Was stirred for 2 minutes.

10. Again within 2 minutes was poured and pressed at an average speed of the washing machine.

11. Remove stains and burden of containers.

12. United all stains and dried in the dryer at medium heat with constant spin cycle for 45 minutes.

13. Dried burden in the dryer at a high temperature for 45 minutes and was removed.

14. Stroked spot at medium heat and put in order.

Classification spots:

Product comparison used a system of evaluation PSU, as described in more detail below. The compositions were tested for efficiency (for example, the residue of a dye substance after washing). In these experiments, washed several tissues with comparable compositions. Spots were evaluated visually by three independent appraisers, who were assigned to units of assessment (PSU) on the scale of the Scheff from 0 to 4:

0. There is no advantage.

1. I think that this product is a little better (not sure).

2. I am sure that the p product a little better.

3. This product is better.

4. This product is much better.

Prepared spots (for example, from tea and wine), was evaluated in a circular system in which spots of the same cycle, reprinted for all treatment options were compared with each other. Consumer test spots (for example, zagrozenia t-shirts and pillow cases) were evaluated in pairs, and compared painted samples, which were divided in half for two different treatments. Then calculate the average value of the processing for each type of spots for each option processing by drawing comparisons of all samples for all treatment options.

B. Effect of pH on affectionate percolate for cleaning

The method of determining the effectiveness of detergent on a small scale was used to determine the effects of pH on affectionate percolate for cleaning consumer dirty t-shirts and pillowcases and prepared stains from tea. Conducted two series of experiments of the eight treatment options in three Parallels to compare the pH range from 5 to 10 with peracetic acid and without it. Used a buffer with low ionic strength, and buffer that chelate metal ions, escaped.

Composition (washing in 2 gallons):

300 ppm C12-C13 E6,5 acetate
+/- 0,5 mm peracetic acid
2,25 ml C12-C13E6,5 acetate
1 ml of 3.78 M peracetic acid (+4M original solution of acetic acid)
1 ml of 7.5 M acetic acid (missing peracetic acid)
5 mm buffer38 ml of 1M (acetate pH 5-6, bicarbonate pH 7.5 to 8.5, the borate pH 9,25 to 10.5)
Water hardness 6 gpgUp to 4.5 ml 10000 gpg 3:1 Ca:Mg
(original hard water solution)
NaOH for pH controlof 7.75 ml of 1N. NaOH (to adjust adding percolate)

Efficiency:

Figure 1 shows the effect of peracetic acid for cleaning consumer polluting stains and prepared stains from tea. As shown in this figure, the total degree of purification of all spots, as a rule, increases with ponizeni pH, in the presence of peracetic acid, and in its absence. The largest positive effect on cleaning, bring peracetic acid was observed at pH 8 and 9, when the difference of cleaning between conditions with peracetic acid and without it was the greatest. This pH corresponds to peracetic acid pKa of 8.2. A positive effect on the clearance of low pH and optimum bleaching peracetic acid pH 8-9 indicates that washing the composition, which covers a wide range of pH, provides improved cleaning efficiency.

EXAMPLE 3

Determination of the parameters create a dynamic pH in the wash

This example describes experiments to determine the parameters of the conditions of washing with dynamic pH. Creating dynamic pH in the wash requires understanding titrated materials in the wash. While the components can be designed to provide dynamic pH, dirty clothes and the average city water have their own buffer tanks, which are much harder to control. Nevertheless, the conducted experiments, in order to carry out these definitions.

A. Titration of contaminated ballast to dynamic pH

Small drums on 2 gallon vertical load filled 2 gallon deionizovannoy water and the hardness of water is brought to 6 gpg using hard water solution from Example 2, A. Added components to the concentrations in the wash 100 ppm LAS, 20 ppm of citrate and 200 ppm of triacetin. At different points in time were added various amounts of PB1 and 1 ppm of perhydrol, in order to obtain different profiles of pH in the wash. Started stirring at 75 rpm and then added 0.6 FUTA consumer contaminated products. Stirring was continued for 20 minutes and all this time nab is time ugali for pH.

Figure 2 shows the different profiles of pH, specific to adding different amounts of perborate, various peligrosas, and at different points in time. The desired pH profile was obtained with 75 ppm PB1 and 1 ppm high perhidrolis (S54V) with the addition of delayed 5 minutes inefficient peligrosas high gidrologiya activity (DT). Profile pH slightly decreased from 9 to 8 during the first 6 minutes, until the first enzyme produced peracetic acid. After 6 minutes the pH had fallen significantly adding enzyme with high hydrolysing activity.

B. Parameters of the substrate and enzyme, creating a dynamic pH

The efficiency gains

Described in Example 2, part a, method of testing the effectiveness of the detergent on a small scale was used to test parametrov substrate and enzyme, creating a dynamic pH on the efficiency of cleaning. Purification was assessed on the consumer dirty t-shirts and pillowcases and prepared the stains of tea, and wine. Conducted an experiment with five treatment options in three Parallels, which add high-performance peligrosas (S54V) and inefficient peligrosas high gidrologiya activity (DT) produced at different points in time during the entire wash cycle. Treatment options were compared and normalized by the commercial the who high-performance liquid (HDL) composition TIDE®.

Composition (washing in 2 gallons):

1 ppm option peligrosas S54V470 ál 16100 ppm option peligrosas S54V (16,1 mg/ml)
1 ppm of perhydrol DT690 ál 11000 ppm of perhydrol DT (11 mg/ml)
300 ppm C12-C15E7 acetate2271 mg C12-C15E7 acetate
200 ppm of triacetin1514 mg of triacetin (~1.3 ml)
2.25 mm hydrogen peroxideof 1.94 ml of 30% H2O2
75 ppm PB1568 mg PB1 (0.75 mm ~pH 10)
20 ppm of citrate1,01 ml of 15% citric acid (0.1 mm ~pH 2)
100 ppm LAS3.98 ml 19% LAS pH 8.5 (neutralized with NaOH)
80 ppm ZPB606 mg ZPB
hardness 6 gpgto 4.5 ml 10000 gpg 3:1 Ca:Mg (initial solution of hard water)
Standard: 1540 ppm TIDE® HDL11,66 g TIDE® HDL

Efficiency:

Figure 3 shows the pH profiles obtained in the region of slichnih conditions. In order to generate sufficient acid to reduce the pH below 6, it was necessary to add peligrosas high gidrologiya activity (DT). Delay adding high-performance peligrosas (S54V) only slightly slowed the decrease in pH, but had a significant effect on the cleaning efficiency. It turned out that this high perhydrol the ability to react with substrates within 5 minutes, the formation of peracetic acid under optimum conditions of activity and then lowering the pH with the help of the enzyme with high gidrologiya activity, create the desired pH profile and the best efficiency of cleaning dirt and wine.

C. Optimizacija parameters of the enzyme for optimum efficiency

The parameters of the enzyme and substrate were optimized using statistical experimental scheme. Described in Example 2, part a, method of testing the effectiveness of the detergent on a small scale was used for testing parameters of the substrate and enzyme in the cleaning efficiency. Purification was assessed on the consumer dirty t-shirts and pillowcases and prepared the stains of tea, and wine. Conducted an experiment with four series of five treatment options in three Parallels, comparing different amounts of highly efficient peligrosas (S54V) with different number is Tami of triacetin and delays in the Appendix. Treatment options were compared and normalized by commercial HDL composition TIDE®.

Triacetin
no experienceAcT S54VTriacetinDelayCode
1130001B
2110001C
30,5530031D
40,120001E
50,32515002B
60,110032C
7 0,32525002D
80,110032E
90,77515033B
100,130003C
110,110003D
120,5520033E
13130034B
140,77525004C
15110034D
160,1 30034E

0.1 to 1 ppm of perhydrol S54V0,1, 0,33, 0,55, 0,78, 1 ml 7570 ppm of perhydrol S54V
100-300 ppm of triacetin757, 1136, 1514, 1893, 2271 mg of triacetin
300 ppm C12-C15E9 acetate2271 mg C12-C15E9 acetate
2 mm hydrogen peroxide1,72 ml of 30% H2O2
100 ppm PB1757 mg PB1 (~pH 10)
20 ppm of citrate152 mg of citric acid
100 ppm LAS3.98 ml 19% LAS pH 8.5 (neutralized with NaOH)
80 ppm ZPB606 mg ZPB
hardness 6 gpgto 4.5 ml 10000 gpg 3:1 Ca:Mg (initial solution of hard water)
Standard: 1540 ppm TIDE® HDL11,66 g TIDE® HDL

Efficiency:

Determined the dependence of the cleaning mud stains, stains from tea and wine from the concentrations of enzyme and triacetin. It turned out that the application of the three-minute detention is key add triacetin to the composition during the wash cycle does not have a significant impact on cleaning any stains. The cleanup of contaminated t-shirts and stains from tea and wine was strongly dependent on the concentration of the enzyme, which indicates that at least 1 ppm, high-performance peligrosas need to turn the greater part of the substrates in the peracetic acid for bleaching and acid to lower pH. No dependence purification of t-shirts and stains from tea on the concentration of triacetin indicates that more than 100 ppm of triacetin not necessary in the present system for washing with only 1 ppm of the enzyme.

The observed dependence of cleaning stains from wine can be explained by the kinetic effect, when the enzyme produces peracetic or acetic acid faster at high concentrations of triacetin and dye wine is more sensitive in the beginning of the wash cycle.

EXAMPLE 3

Determination of substrate and its effect on the cleaning efficiency

This example describes experiments to determine the optimal substrate. The substrates peligrosas used in the detergent composition with dynamic pH to produce percolate and acid for bleaching and lowering pH during the wash cycle.

Triacetin is a water-soluble substrate, with a high ratio of the number of moles of acid to the weight for the production of large quantities of peracetic acid and acetic acid in solution. In other vari is ntah implementation in providing superior cleaning are used surface-active esters, because they combine the properties of surface-active substance with a complex ether, which can be converted during cleaning perhidrolis in peracetic acid. Four surface-active complex ester were tested for their effects on cleaning stains on t-shirts and pillowcases prepared stains from tea and wine. Four surface-active complex ester include alkyl chains of different lengths with different lengths of chains of ethylene oxide and attached to the terminal primary alcohol last ethoxylate complex ester of acetic acid.

C12-C13E9 acetate consists of an alkyl chain distribution center is about 12 to 13 carbon atoms, distribution amoxilonline with the center approximately 9 units of ethylene glycol and terminal acetate. C12-C15E7 acetate consists of an alkyl chain of from 12 to 15 carbon with 7 units of ethylene oxide and acetate. C9-C11E2,5 acetate consists of an alkyl chain of from 9 to 11 carbons with from 2 to 3 units of ethylene oxide and acetate. C9-C11E6 acetate consists of an alkyl chain of from 9 to 11 carbon with 6 units of ethylene oxide and acetate.

Described in Example 2, part a, method of testing the effectiveness of the detergent on a small scale was used for testing these substrates on the efficiency of cleaning, is using pilot scheme with five treatment options in three Parallels. Treatment options were compared and normalized on commercial high-performance liquid composition TIDE®.

Composition (washing in 2 gallons):

1 ppm of perhydrol S54V1 ml 7570 ppm of perhydrol S54V
300 ppm of various surface-active esters2271 mg C12-C13-E9, C12-C15-E7, C9-C11-E2,5, C9-C11-E6,5 acetates
200 ppm of triacetin1514 mg of triacetin (~1.3 ml)
2.25 mm hydrogen peroxideof 1.94 ml of 30% H2O2
75 ppm PB1568 mg PB1 (0.75 mm ~pH 10)
20 ppm of citrate152 mg of citric acid (0.1 mm ~pH 2)
100 ppm LAS3.98 ml 19% LAS pH 8.5 (neutralized with NaOH)
80 ppm ZPB606 mg ZPB
hardness 6 gpgto 4.5 ml 10000 gpg 3:1 Ca:Mg (initial solution of hard water)
Standard: 1540 ppm TIDE® HDL11,66 g TIDE® HDL

Efficiency:

N the Figure 3 shows the profiles of pH, obtained with different substrates. Any changes peligrosa or hydrolysis of substrates by the enzyme, or the ratio of the number of moles of acid to the weight did not influence significantly on the pH profiles. C12-C15-E7 acetate, however, provided a slightly better cleaning consumer dirty t-shirts and pillowcases. Shorter substrates, C9-C11-E2,5 and C9-C11-E6 acetates, provide better cleaning hydrophilic stains of tea and wine, probably due to the higher ratio of the number of moles of percolate-to-weight ratio. In any case, all the substrates in combination with the enzyme was well cleaned spot of tea and wine.

EXAMPLE 4

Comparison of detergent compositions with dynamic pH with commercial brands

This example describes experiments to compare detergent compositions with dynamic pH. Described in Example 2, part a, method of testing the effectiveness of the detergent on a small scale was applied in order to compare the cleaning performance of detergent compositions with dynamic pH with commercial brands TIDE®. Purification was assessed on the consumer dirty t-shirts and pillowcases and prepared the stains of tea, and wine. Conducted an experiment with five treatment options in three Parallels, comparing commercial Liquid TIDE® with Bleach Alternative, commercial TIDE® with bleach granules, to the position with a dynamic pH, containing C12-C15-E7 acetate composition with dynamic pH containing C9-C11-E2,5 acetate, and KOMMERCHESKIY TIDE® HDL composition as a reference. For compositions with dynamic pH was added protease, in order to equalize all the advantages of commercial brands on the spots containing proteins, such as consumer contaminated products. Inefficient peligrosas high gidrologiya activity (DT) was added to the wash cycle option processing dynamic pH after a five-minute delay to produce the optimum pH profile using real components. In these experiments also used a serine protease ASP.

Composition (washing in 2 gallons):

1 ppm variant ASP R18298 μl 25400 ppm ASP R18
1 ppm of perhydrol S54V470 ál 16100 ppm of perhydrol S54V
1 ppm of perhydrol DT after a five-minute delay690 ál 11000 ppm of perhydrol DT 5 minute wash cycle
300 ppm of various ester substrates2271 mg C9-C11-E2,5, C12-C15-E7 acetates
200 ppm of triacetin 1514 mg of triacetin (~1.3 ml)
2.25 mm hydrogen peroxideof 1.94 ml of 30% H2O2
75 ppm PB1568 mg PB1 (0.75 mm ~pH 10)
20 ppm of citrate1,01 ml of 15% citric acid (0.1 mm ~pH 2)
100 ppm LAS3.98 ml 19% LAS pH 8.5 (neutralized with NaOH)
80 ppm ZPB606 mg ZPB
hardness 6 gpgto 4.5 ml 10000 gpg 3:1 Ca:Mg (initial solution of hard water)
Standard: 1540 ppm TIDE® HDL11,66 g TIDE® HDL
1540 ppm Liquid TIDE® with Bleach Alternative (LTBA)11,66 g LTBA
970 ppm granular TIDE® with bleach7,34 g TIDE® with bleach

Efficiency:

Figure 3 shows the pH profiles obtained in different conditions. Composition with dynamic pH, regardless of the substrate, creates a linear profile of pH from pH 9 to 5.5 during the entire wash cycle. The pH profile of commercial formulations TIDE® slowly decreased after the addition of test spots and contaminated ballast because of the inherent buffering capacity, but pH ostavlennym throughout the wash cycle. Liquid compositions TIDE® supported in the wash pH 7.5, whereas granular TIDE® with bleach supported in the wash pH of 10.25. Detergent compositions with dynamic pH is much better than commercial liquid formulations TIDE®, coped with cleaning consumer dirty t-shirts and pillowcases and prepared stains from tea and wine. Best of compositions with dynamic pH with C12-C15-E7 acetate as substrate showed himself to be equivalent granular TIDE® with bleach on the consumer contaminated products, and stains from tea and wine.

EXAMPLE 5

Detergent composition

The following example provides examples of various detergent compositions. In these compositions the number of enzymes is expressed as the ratio of the pure enzyme by weight of the entire composition and, if not agreed otherwise, the ingredients of the detergent is expressed in relation to the weight of the entire composition.

Get the following detergent compositions of the present invention for washing.

td align="left"> 0,5 0,08
IIIIIIIVV
LASto 12.0-4,0- -
C12-C15AEa 1.8S-2,03,08,05,0
C8-C10propylpiperonyl2,02,02,02,01,0
C12-C14alkyldimethylammonium----2,0
C12-C15AS-10,0-2,02,0
CFAA-5,04,04,03,0
MCAEM
(triacetin)
to 12.06,015,020,015,0
C12-C18fatty acids8,06,0 2,02,02,0
Citric acid (anhydrous)2,01,01,51,01,0
DETPMP1,01,01,01,00,5
Monoethanolamine8,06,03,03,02,0
Bicarbonate5,03,5-2,5-
Propandiola 12.714,513,110,8,0
Ethanol1,81,8the 4.7of 5.41,0
Pectinase--- -
Amylase-0,002--
Cellulase--is 0.00020,0001
Lipase0,1-0,1-0,1
Protease0,050,30,0550,50,2
Alasoorituse--0,3-0,003
PAAC0,010,01---
DETBCHD--0,020,01-
SRP10,5-0,30,3
Boric acid2,42,42,82,82,4
Ecological sodium--3,0--
DC3225 C1,01,01,01,01,0
2-butyloctyl0,030,040,040,030,03
DTPA0,50,40,350,280,4
Brightner 10,180,100,11--
Peligrosas0,050,30,50,2
MCAEM
(C12-C13E6,5acetate)
3,08,0to 12.01,54,8
Brought to 100% perfume/dye and/or water

the pH of the compositions (I) to (V) is from about 9 to about 10, and it lead up to this value by adding sodium hydroxide.

In addition, you receive the following liquid detergent compositions of the present invention for hand washing of dishes.

IIIIIIIVVVI
C12-C15AEa 1.8S20,0to 12.010,0-10,010,0
LAS---5,05,08,0
Parafusulina---to 12.0--
C10-C18alkyldimethylammonium5,03,05,0---
Betaine3,0-1,03,01,0-
Amide C12-poly-OH fatty acids---3,0-1,0
Amide C14-poly-OH fatty acids-1,5----
MCAEM
(triacetin)
to 12.0151881520,0
DTPA----0,2-
Trinatriytsitrat the dihydrate0,25--0,7--
Diamine1,05,07,01,05,07,0
MgCl20,25--1,0--
Protease0,020,010,020,010,020,05
Amylase0,001--0,002-0,001
Alasoorituse- --0,020,050,01
Coolcullen sodium---2,01,53,0
PAAC0,010,010,02---
DETBCHD---0,010,020,01
PB11,52,81,2---
Peligrosas0,020,010,030,010,020,05
MCAEM
(C11-E9acetate)
3,42,84,04,66,8
Brought to 100% perfume/dye and/or water

the pH of the compositions (I) to (VI) is from about 8 to about 9, and it lead up to this value by adding sodium hydroxide.

Also get the following liquid detergent compositions of the present invention for automatic dishwashing.

IIIIIIIVV
STPP1616181616
Potassium sulfate-108-10
1,2-propandiol6,00,52,06,00,5
Boric acid4,03,03,0 4,03,0
CaCl2the dihydrate0,040,040,040,040,04
MCAEM
(triacetin)
5,03,0to 12.08,01,0
Protease0,030,030,030,030,03
Amylase0,02-0,020,02-
Alasoorituse-0,150,02-0,01
Galactosidase--0,01-0,01
PAAC0,01--0,01 -
DETBCHD-0,01--0,01
Peligrosas0,10,030,050,030,06
MCAEM
(C14-C15E12acetate)
1,00,51,01,00,5
Brought to 100% perfume/dye and/or water

the pH of the compositions (I) to (V) is from about 9 to about 10, and it lead up to this value by adding sodium hydroxide.

Also get the following detergent compositions of the present invention for washing clothes. These compositions are in the form of granules or tablets in some preferred versions of the implementation.

10,0
IIIIIIIVV
The main productthe
C14-C15AS or TAS8,05,03,03,03,0
LAS8,0-8,0-5,0
C12-C15AE3S0,52,01,0--
MCAEM
(triacetin)
to 12.015,010,018,0to 12.0
QAS---1,01,0
Zeolite And5,08,06,0-5,0
SKS-6 (add dry)- -4,0--
MA/AA2,02,02,0--
AA----4,0
3Na citrate 2H2O-2,0---
Citric acid (anhydrous)2,0-1,52,0-
DNPA0,20,2---
EDDS--0,50,1-
HEDP--0,20,1 -
PB13,04,8--4,0
Bicarbonate--the 3.85,2-
NOBS1,9----
NASA OBS--2,0--
TAED0,52,02,05,01,00
BB10,06-0,34-0,14
BB2-0,14-0,20-
Sulfate20,025,025,018,0
Silicate-1,0--3,0
Protease0,0330,033---
Protease--0,0330,0460,033
Lipase-0,008---
Amylase0,001---0,001
Cellulase-0,0014---
Pectinase0,0010,0010,0010,001 0,001
Alasoorituse0,03-0,05--
PAAC-0,01--0,05
Peligrosas0,030,051,00,060,1
MCAEM**2,05,0to 12.03,56,8
Brought to 100% moisture/sodium sulfate and/or minor components*

• Perfume / dye, Breitner / SRP1 / Na carboxymethylcellulose / photouploader / MgSO4/ PVPVI / defoamer / high molecular weight PEG / clay.

• ** MCAEM selected from the group consisting of C9-C11-E2,5-acetate, [C12H25N(CH3)(CH2CH2OAc)2]+Cl-, (CH3)2NCH2CH2OCH2CH2OAc or mixtures thereof.

Also get the following liquid detergent compositions of the present invention for washing and Laundry.

TPKFA
IIIIIIIIVV
LAS11,58,59,0-4,0-
C12-C15AE2,85S--3,018,0-to 12.0
C14-C15Ethe 2.5S8,511,53,0-to 12.0-
MCAEM
(triacetin)
3,23,23,02,02,01,0
CFAA---5,0-3,0
2,02,0-2,00,52,0
Citric acid (anhydrous)3,23,20,51,22,01,2
Format Ca0,10,10,060,1--
Format Na0,50,50,060,10,050,05
The Na cumene sulfonate4,04,01,03,01,2-
Borat0,60,6-3,02,03,0
Ethanol2,0 2,01,04,04,03,0
1,2-propandiol3,03,02,08,08,05,0
Monoethanolamine3,03,01,51,02,51,0
TEPAE2,02,0-1,01,01,0
PB1the 3.82,04,53,22,82,5
Protease0,030,030,010,030,020,02
Lipase---0,002 --
Amylase----0,002-
Cellulase-----0,0001
Pectinase0,0050,005---
Alasoorituse0,05--0,05-0,02
Galactosidase-0,04
Peligrosas0,030,050,010,030,080,02
MCAEM
(C11-C15-E6acetate)
3,24,61,83,56,22,8
PAAC0,030,030,02---
DETBCHD---0,020,01-
SRP 10,20,2-0,1--
DTPA---0,3--
PVNO---0,3-0,2
Brightner 10,20,2 0,070,1--
Silicone non0,040,040,020,10,10,1
Brought to 100% perfume/dye and/or water

the pH of the compositions (I) to (VI) is from about 9 to about 10, and it lead up to this value by adding sodium hydroxide.

Get the following compact high-density detergents of the present invention for washing dishes.

BTA
IIIIIIIVVVI
STPP-35,045,0--20,0
3Na citrate 2H2Oof 17.0--30,035,0 -
Silicate5,05,03,0-5,01,0
Metasilicate2,54,54,5---
PB1--4,5---
PB4---5,0--
Percarbonate5,04,5--the 3.84,8
BB1-0,10,1-0,5-
BB20,20,05 -0,1-0,6
MCAEM
(triacetin)
3,514,55,53,02,925,9
HEDP1,0-----
DETPMP0,6-----
PAAC0,030,050,02---
Paraffin0,50,40,40,6--
Protease0,0720,0530,0530,0260,059
Amylase0,012-0,012-0,0210,006
Lipase-0,001-0,005--
Pectinase0,0010,0010,001---
Alasoorituse0,050,050,030,010,020,01
Peligrosas0,0720,0530,0530,0260,0590,01
MCAEM
(C12-C13-E6,5acetate)
3,52,81,67,54,20,8
0,30,20,20,30,30,3
Polycarboxylate6,0---4,00,9
Perfume0,20,10,10,20,20,2
Brought to 100% moisture/sodium sulfate and/or minor components*

* Breitner / dye / SRP1 / Na carboxymethylcellulose/ photouploader / MgSO4/ PVPVI/ defoamer / high molecular weight PEG / clay.

the pH of the compositions (I) to (VI) is from about 9.0 to approximately 10.0.

The following detergent compositions of the present invention in the form of tablets obtained by pressing granular detergent composition for washing dishes at a pressure of 13 kN/cm2using standard rotary press with 12 heads.

td align="left"> 0,5
III IIIIVVVIVIIVIII
STPP-38,824,728,2-22,426,116,0
3Na citrate 2H2O20,0---35,9---
Carbonate Na5,01,03,02,41,05,02,03,0
Silicate5,04,85,02,63,41,92,31,2
Lipase0,001- 0,01-0,02---
Protease0,0420,0720,0420,031----
Protease C----0,0520,0230,0230,029
Peligrosas0,010,080,050,040,0520,0230,0230,029
MCAEM
(C9-C11-Ethe 2.5acetate)
2,86,54,5the 3.84,62,82,82,8
Amylase0,012 0,0120,012-0,015-0,0170,002
Pectinase0,005--0,002----
Alasoorituse-0,03-0,020,02-0,03-
PB1--the 3.8-7,8--8,5
Percarbonate6,0the 3.8-6,0-5,04,5-
BB10,2--0,30,2--
BB2-0,2-0,5--0,10,2
MCAEM
(triacetin)
3,54,04,05,23,04,24,06,5
PAAC0,010,010,02-----
DETBCHD---0,020,02---
TAED---- -2,1-1,6
HEDP1,0--0,9-0,40,2-
DETPMP0,7-------
Paraffin0,40,50,50,5--0,5-
BTA0,20,30,30,30,30,30,3-
Polycarboxylate4,0---4,9 0,60,8-
PEG 400-30000-----2,0-2,0
Glycerin-----0,4-0,5
Perfume---0,050,20,20,20,2
Brought to 100% moisture/sodium sulfate and/or minor components*

* Breitner / dye / SRP1 / Na carboxymethylcellulose/ photouploader / MgSO4/ PVPVI/ defoamer / high molecular weight PEG / clay.

the pH of the compositions (I) to 7(VIII) is from about 9 to about 10.

The weight of the tablet compositions with 7(I) 7(VIII) is from about 20 grams to about 30 grams the century

Get the following liquid detergent compositions of the present invention for cleaning hard surfaces.

IIIIIIIVVVIVII
MCAEM
(triacetin)
7,05,98,512,515,56,412,5
LAS---0,80,8-0,8
Coolcullen sodium1,52,6-1,51,51,51,5
Isachem ® AS0,60,6---0,6 -
3Na citrate 2H2O0,50,560,50,60,750,50,75
Fatty acid0,60,130,60,10,40,60,4
2-butyloctyl0,30,3-0,30,30,30,3
PEG DME-2000®0,4-0,30,350,5--
PVP0,30,40,60,30,5--
MME PEG (2000)®-- --0,50,5
Jeffamine ® ED-2001-0,4--0,5--
PAAC---0,030,030,03-
DETBCHD0,030,050,05----
Protease0,070,050,050,030,060,010,04
Amylase0,120,010,01-0,02-0,01
Lipase -0,001-0,005-0,005-
Peligrosas0,070,050,080,030,060,010,04
MCAEM
(C12-C15-E6acetate)
3,55,64,85,33,68,0the 4.7
Pectinase0,001-0,001---0,002
PB12,54,61,8the 3.83,21,82,8
Alasoorituse0,05-0,03 -0,020,020,05
Brought to 100% perfume/dye and/or water

the pH of the compositions (I) to (VII) is from about 8.5 to about 9.5, and it lead up to this value by adding sodium hydroxide.

All mentioned in the specification of the patents and publications indicate the level of experts in the field of technology, which corresponds to the present invention. All patents and publications is incorporated into this description by reference as if each individual publication was specifically and individually marked as included in the reference.

If that describes the preferred embodiments of the present invention, for specialists in the art it is clear that there can be produced various modifications of the described embodiments, it is assumed that such modifications are included in the scope of the present invention.

Specialists in the art will easily understand that the present invention is well adapted to accomplish the goals and to achieve those mentioned, as well as its inherent, results and benefits. The compositions and methods described in the present description, are typical representatives of the pre is respectful of embodiments, are the samples and were not intended as limitations of the scope of the present invention. Specialist in the art will easily understand that described in the present description the invention can be made of various substitutions and modifications without deviating from the scope and essence of the present invention.

The invention is clearly described in the present description, suitable for implementation in practice in the absence of any element or elements, limitation or limitations, not specifically disclosed in the present description. Used terms and expressions have been used as terms of description and not of limitations, and application of these terms and expressions there is no intention to exclude any equivalents shown and described signs or portions thereof, but it should be understood that various modifications are possible within the scope of the present claimed invention. Thus, it should be understood that although the present invention specifically disclosed by preferred embodiments and optional features, expert in the art may resort to modification and change of concepts described in the present description, and that such modifications and changes are considered as lying within the scope of the present invention, as defined by the attached claims.

This is the invention described in the present description widely and in General. Each of the narrower species and subradular groups falling under the generic description, also forms part of the present invention. This includes the generic description of the present invention with the exception or negative limitation excluding any object of the kind, regardless of whether listed specifically excluded material in the present description or not.

1. The method of cleaning at least part of the surface and/or tissue, including: an optional stage of the wash and/or rinse the surface and/or tissue; contacting surface and/or fabric with a detergent solution containing pergidrolya the enzyme and the substrate for a given enzyme, where the initial pH of the washing solution is alkaline and the number pelikulang enzyme and substrate is sufficient to lower the pH of the washing solution to 6.5 or below; and optionally washing and/or rinsing the surface and/or tissue, where the specified bringing into contact takes place during washing cycle, and where the lowering of the pH of the washing the solution improves the efficiency of the washing solution.

2. The method according to claim 1, wherein lowering the pH from alkaline to acid improves the efficiency of the washing solution with optimal activity at acidic pH.

3. The method according to claim 2, in which the component is an enzyme.

4. The method according to claim 1, which is a decline from alkaline pH to acidic pH increases the stain removal compared to removing at alkaline pH.

5. The method according to claim 1, in which the specified pH of the washing solution is reduced essentially linearly.

6. The method according to claim 1, in which the specified pH of the washing solution is reduced to 6.5 or below during the last 25-50% of a given wash cycle.

7. The method according to claim 1, in which the specified surface and/or fabric is exposed to the specified cleaning solution having a pH below about 6.5, for at least about 2 minutes



 

Same patents:

FIELD: chemistry.

SUBSTANCE: composition contains more than 5% anionic surfactant, less than 25% nonionic surfactant, a light-sensitive ingredient and an inorganic mother-of-pearl agent. The light-sensitive ingredient is selected from a group comprising enzymes, dyes, vitamins, aromatising agents and mixtures thereof.

EFFECT: inorganic mother-of-pearl agent improves stability of light-sensitive ingredients in the detergent composition.

20 cl, 14 ex

FIELD: chemistry.

SUBSTANCE: invention discloses a composition which contains a fabric toning agent and a bacterial alkaline enzyme which exhibits endo-beta-1,4-glucanase activity (E.C.3.2.1.4). The fabric toning agent meets Testing method 1 requirements given in the description. Described also is a method of cleaning and/or processing a surface or fabric using the said composition.

EFFECT: composition ensures better removal of yellow stains and improved deposit of the agent with improvement of the outer appearance of the fabric.

18 cl, 16 ex

FIELD: chemistry.

SUBSTANCE: cleaning compositions contain organic catalysts having improved enzyme compatibility and having the following formulae: where each R1 independently denotes a branched alkyl group selected from a group comprising 2-butyloctyl, 2-pentylnonyl, 2-hexyldecyl, isodecyl, isotridecyl and isopentadecyl, or a linear alkyl group containing 11-18 carbon atoms. The composition also contains one or more auxiliary ingredients.

EFFECT: cleaning compositions with efficient bleaching at low water temperature.

15 cl, 16 ex

FIELD: chemistry.

SUBSTANCE: present invention relates to a composition which contains lipase, and a bleaching catalyst which can accept an oxygen atom from a peroxy acid and transfer the oxygen atom to an oxidisable substrate.

EFFECT: less bad smell which arises when using lipase enzymes in detergents.

13 cl, 4 tbl, 3 ex

FIELD: chemistry.

SUBSTANCE: detergent composition contains a polymer combined with a surfactant or an inorganic detergent component and auxiliary ingredients. The fat purification efficiency index of the detergent composition is at least equal to 10. The polymer is a random grafted copolymer which has a hydrophilic skeleton and hydrophobic side chains, obtained by grafting (a) polyethylene oxide; (b) vinyl ester of acetic and/or propionic acid; and/or C1-4alkyl ester of acrylic or methacrylic acid; and (c) modifying monomers. The detergent compositions preferably additionally contain lipase enzyme.

EFFECT: improved removal of fat contaminants and stains with reduced amounts of conventional surfactants or inorganic detergent components.

22 cl, 6 ex

FIELD: chemistry.

SUBSTANCE: compositions contain certain versions of lipase and a photo-bleaching agent based on xanthene dye, a photoinitiator and their mixtures.

EFFECT: more efficient cleaning, leading to minimal undesirable bad smells.

11 cl, 3 tbl, 16 ex

FIELD: chemistry.

SUBSTANCE: present invention relates to compositions which contain certain versions of lipase and a toned agent for fabric, and includes use of such compositions for cleaning and/or treating surface areas or fabric.

EFFECT: improved deposition of temporary colourant, reduced activity of the enzyme which leads to bad smell, and improved perception of cleanness.

17 cl, 3 tbl, 16 ex

FIELD: chemistry.

SUBSTANCE: detergent contains the components as follows, wt %: anionic surface active agent (SAG) - alkylbenzol sodium sulphonate 10-16; nonionic SAG - oxyethylated fatty alcohols or oxyethylated alkylphenol 2-5; sodium tripolyphosphate 15-25; organophosphonate compound - sodium salt 1-hydroxyethylidene of phosphonic acid or sodium diethylentriaminopentaxys-(methylene phosphonate) 0.2-0.6; polycarboxylate 0.5-1.5; carboxymethyl cellulose 0.3-0.6; modified polyalkylene glycol 0.2-0.6; optical bleaching agent 0.05-0.3; soda ash 3-6; liquid glass 3.5-6.0; enzyme 0.4-0.7; defoaming agent 0.05-1.5; aromatiser 0.15-0.3; sodium sulphate and water to 100.

EFFECT: higher efficiency of bleaching detergent, all type decontamination with additional softening of fabric and without irritation of hand skin during manual washing, lower temperature modes of washing, reduced damage effect on metal parts of washing machines.

10 cl, 2 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: invention is meant for soaking, washing and bleaching all types of textile articles, except articles from natural silk and wool, in any kind of machine, and also hand wash. Substance contains in % mass: an anionic surfactant 7-15, nonionic surfactant oxyethylated fatty alcohol 2-5, sodium tripolyphosphate 15-25, sodium ethylene-diaminotetraacetate (versene) 0.3-1, polycarboxylate, containing monomers of acrylic acid 0.2-0.6, carboxymethyl cellulose (CMC) (in terms of 100% of the share of the main substance) 0.4-0.6, sodium silicate (in terms of SiO2) - 2-5, sodium perborate or sodium percarbonate (in terms of activated oxygen) 1.5-4, tetraacetylethylenediamine (TAED) 0.5-3, enzymes 0.4-1, caustic ash 5-15, mixture of sodium carbonate and amorphous sodium silicate 2-5, optical brightener 0.05-0.4, perfume 0.1-0.3, sodium sulphate and water till 100.

EFFECT: increase in the effectiveness of the detergent with a whitening effect, removal of all kinds of dirt while adding extra softness without an irritating effect on the skin during hand washing, reducing the temperature regimes of washing, reduction in the harmful effect to the metallic part of the washing machine.

9 cl, 2 tbl

FIELD: chemistry.

SUBSTANCE: invention pertains to compositions of granulated synthetic detergents, meant for manual and machine washing (in any type of machine) and soaking all types of textiles, including coloured and other household needs. The detergent contains the following components in the given % mass: anionic surface active agent sodium alkylbenzenesulfonate 9-12; non-ionic surfactant 2-5; sodium tripolyphosphate 15-30; dispergator 0.3-1.2; carboxymethyl cellulose 0.3-0.7; sodium silicate 3-6; modified polyalkylene glycol 0.2-0.6; enzyme 0.3-0.8; sodium carbonate 6-12; photo-bleaching agent 0.003-0.020; optical brightener 0.05-0.20; fragrance component 0.1-0.3; sodium sulphate and water up to 100. The non-ionic surfactant preferably contains oxyethylated monoalkylphenols based on propylene trimers or oxyethylated fatty alcohols. The dispergator used is sodium polyacrylate or sodium salt of a copolymer of maleic or acrylic acid or a sodium salt of a copolymer of acrylic, methacrylic and maleic acid. Alternatives of the synthetic detergent are also described.

EFFECT: increased effectiveness of the detergent, bleaching effect after washing, wider assortment of high quality granulated synthetic detergents.

7 cl, 1 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to oil-containing starch granules for delivering perfume oil as beneficial effect endowing additives to a substrate, containing: (a) starch in amount which forms an effective matrix for said granules; (b) perfume oil containing ingredients with Clog P equal to at least 3; and (c) an effective amount of an amidoamine compound for inhibiting migration of said oil to the surface of the said starch granules, said compound has the following structure: (I) or (II), where radicals are described in the claim separately for each structure. The invention also relates to a method of producing oil-containing starch granules, comprising the following steps: (a) preparing a dispersion of starch in water to form a starch suspension; (b) melting an effective amount of the amidoamine compound of structure (I) or (II) to obtain a molten amidoamine compound; (c) adding perfume oil to the molten amidoamine compound from step (b) to obtain a solution of the amidoamine compound in perfume oil; (d) adding the solution from step (c) to the starch suspension from step (a); (e) homogenisation of the obtained suspension by mixing to obtain a homogeneous mixture; and (f) spray drying the said homogeneous mixture to obtain oil-containing starch granules. The invention also relates to a method of washing fabric, comprising the following steps: (a) preparation of an aqueous solution containing an effective amount of the oil-containing starch granules in claim 1 or 2, and (b) bringing the fabric to be washed into contact with the aqueous solution from step (a). The invention also pertains to a laundry detergent composition containing: (a) at least one surfactant; and (b) an effective amount of oil-containing starch granules.

EFFECT: prolonged existence of the aromatising agent in the substrate owing to inhibition of migration of perfume oil to the surface during laundry.

7 cl, 5 tbl

FIELD: textile industry.

SUBSTANCE: lightproof composition added in process of cloth rinsing has viscosity of less than 500 mPas after at least one cycle of freezing-unfreezing and contains from approximately 5 to approximately 30 wt % of active component, more than 5 wt % of polyol, which represents polyatomic alcohol that is not ethylene glycol, propylene glycol, diethylene glycol or dipropylene glycol and 0.1-10 wt % of additional softener selected from hydrophobised ether of saccharose, cationic starch, aminofunctional silicon or their mixtures. Active component represents compound or mixture of compounds, having the following formula: {R4-m-N+-[(CH2)n-Y-R']m}X-, (a), where each R is hydrogen, short chain C1-C6, poly(C2-3 alkoxy), benzyl, or their mixtures; m = 2 or 3; n = 1-4; Y = -O-(O)C- or -C(O)O; sum of carbon atoms in each R1=C11-C21, for each R1, which represents hydrocarbyl or substituted hydrocarbyl group; and X-anion compatible with softener; or {R4-m-N+-[(CH2)n-Y-R']m}X-, (b), where each R is hydrogen, short chain C1-C6, poly (C2-3alkoxy), benzyl, or their mixtures; m = 2 or 3; n =1-4; Y=CH2, -NR-C(O)-, or -C(O)-NR- and each Y is same or different; sum of carbon atoms in each R1 minus (n+1), when Y is CH2, is equal to C12-C22, for each R1, which represents hydrocarbyl or substituted hydrocarbyl group; and X- any anion compatible with softener; or (c), where each R, R1, and A- have values given above; each R2 -C1-C6 alkylene group; and G - atom of oxygen or -NR-.

EFFECT: softener has good dispersive ability and spreading property when stored at low or high temperature.

9 cl, 1 tbl, 4 ex

FIELD: biochemistry; production of the water purifying diphasic compositions.

SUBSTANCE: the invention is pertaining to the water purifying diphasic compositions. The composition contains (in mass %): (a) 5-75 of the surface-active substance; (b) at least 2.5 molecules of polydextrose; (b) at least, 2.5 - 50 molecules of sucrose; and (d) - the water and auxiliary components - up to the balance. The other version is the composition containing (in mass %): (a) 5-75 of the surface-active substances; (b) at least - 2,5 molecules of molecules of polydextrose; (c) at least from 0.5 up to approximately 4 the salt; (d) at least - 2.5 - sucrose; and (e) the water and the auxiliary components to the balance. These compositions contain, at least, two visible separated layers on the basis of the water at aging the compositions without shaking or steering. The technical result of the invention is the increased stability.

EFFECT: the invention ensures the increased stability of the water purifying diphasic compositions.

12 cl, 3 tbl, 14 ex

FIELD: agriculture; chemical industry; other industries; production of the water two-phase purifying compositions.

SUBSTANCE: the invention is pertaining to the water purifying two-phase compositions. The composition contains: (a) about 5-75 % of the mass of the surface-active substance, (b) at least about 15 % of the molecule or the molecules of the polydextrose, where the polymerization degree makes from the nearby 4 to the nearby 22,(that corresponds to the molar weight from nearby 600 to nearby 3600) and (c) the water and the auxiliary components to the balance. The composition contains at least two visible separated layers on the basis of water at keeping without stirring or hashing. The composition containing from 0.5 up to nearby 3 % of the salt, contains at least about 10 % of the polydextrosew for induction of the two-phase formation. The technical result of the invention is the increase of stability.

EFFECT: the invention ensures the increase of stability of the water purifying two-phase compositions induced by the polysextrose.

10 cl, 4 tbl, 15 ex

FIELD: aqueous composition for fabric softening.

SUBSTANCE: claimed composition contains (mass %): (i) at least one cationic compound - fabric softening agent, having two or more alkenyl chains wherein each chain contains 8 or more carbon atoms; and (ii) at least one sugar oil derivative. Cationic compound (i) and/or sugar oil derivative (ii) are individually mixed with other active component of fabric softening composition, excluded anionic surfactants, water, paints, conserving agents or other optional components with small concentration, to provide intermediate mixture, followed by mixing of cationic compound (i) and sugar oil derivative (ii). Aqueous fabric softening composition produced by claimed method and method for fabric treatment using the same, also are disclosed.

EFFECT: homogeneous composition with improved cleavage resistance.

12 cl, 9 tbl, 16 ex

The invention relates to foaming detergent compositions that can be applied in individual detergents produced in the form of viscous liquids, creams or gels

The invention relates to a molded product, the detergent or cleaning action, primarily tablets, and method of production thereof

The invention relates to a method of cleaning vehicles, which consists in spraying the vehicle alkaline solution washing composition in a mixture with an aqueous solution of a derivative of cellulose

FIELD: chemistry.

SUBSTANCE: liquid contains the following in wt %: isopropyl alcohol 10-30, ethyl carbitol 2-10, nonionic surfactant 0.01-0.10, polyatomic alcohol 0.02-0.10, substance, water - the rest. The polyatomic alcohol is preferably glycol and the amino alcohol is triethanolamine.

EFFECT: low freezing point of aqueous solutions of isopropyl alcohol, high cleaning power, low total content of low-temperature ingredients and cost of the product.

3 cl, 2 tbl, 2 ex

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