Method for producing preparation of boar natural sex pheromones

FIELD: medicine.

SUBSTANCE: invention refers to veterinary science, particularly to production of a preparation of boar natural sex pheromones. A method for producing the preparation of boar natural sex pheromones, involving mixing pericarps, root of celeriac, root of parsnip in certain proportions, grinding, homogenising, adding the enzyme protosubtiline, incubating, centrifuging, mixing with urine of senior boars in certain proportions, pressing out and sublimating the produced fluid.

EFFECT: method allows providing higher yield of the preparation with better biological activity.

4 tbl, 3 ex

 

The invention relates to the field of veterinary medicine, in particular the production of biological drugs, which are used in pig production as a stimulant of sexual function in sows.

A method of obtaining the drug of sex pheromones from the urine of a bull (Ed. St. No. 1666102, 1991 "Method of production of sex pheromones", ed. Kononov VP and others) and the method for the preparation of the natural sex pheromone of the boar (Patent No. 2034521, 1992 "a Method of obtaining drug natural sex pheromone of the boar", ed. The Seine O., and others). However, these methods difficult to implement, require special equipment and qualified maintenance. The obtained preparations of sex pheromones have low biological activity.

The closest technical solution, selected as a prototype, is a method for the preparation of the natural sex pheromone of the boar (Patent No. 2159597, 1999, "Method of obtaining drug natural sex pheromones of males", ed. The Seine O., and others), which consists in mechanical grinding testes obtained from sexually Mature males to versionrange state, followed by homogenization until smooth. To increase the destruction of cellular tissue and release pheromones into the solution, in homogenised contribute enzyme it (GSH GOST 23636-79) in an amount of 1-1,5% of the total mA the son of the feedstock and incubated the mixture at a temperature of 37-39°C for 50-60 min, and then centrifuged to separate it into two fractions. The supernatant is mixed with the urine of a male in the ratio of 1:2-2,5 and distilled with steam. The resulting condensate is the product of the natural sex pheromone of the male. Shelf life 12 months.

The disadvantage of this method is the low yield of the final product, is relatively low biological activity, a short retention period.

The aim of the invention is to increase the yield of product with high biological activity and a long shelf life.

This goal is achieved by the fact that the quality of raw materials used testes and urine sexually Mature boars, and celery root, and the root of the parsnip, containing the steroid 5α-androst-16-EN-3-one and 5-α-androst-16-EN-3α-ol, which is the basis of the sex pheromone of the boar. Testes and celery and parsnips in the ratio of 1.0:0.25 to:0,25 crushed to versionrange state and homogenized in the homogenizer until smooth. Then homogenized contribute enzyme it (GSH GOST 23636-79) in an amount of 1.0 to 1.5% of the total weight of the feedstock. The resulting mixture was incubated in a thermostat at a temperature of 37-39°C for 50-60 minutes, then mixed with urine sexually Mature boars in the ratio of 1:1.0 to 1.5 and press using a press with cells of 0.7×0,7 mm Poluchenno the liquid is bottled in glass bottles, in 15.0 ml and subjected to sublimation. After sublimation vials closed with rubber stoppers and running aluminum caps.

The optimal ratio of these components were selected with the use of mathematical planning of experiment method the steep ascent. If you increase the amount of urine is reduced concentrations of 10-15%, with a decrease in urine drug get 25-30% less than at the optimum ratio of components.

Optimum incubation was chosen empirically. At temperatures below 37°C and above 39°C there is a decrease of enzyme activity of protocolin, resulting in the hydrolysis process proceeds insufficient, resulting in reduction of yield of the drug. When the duration of incubation, less than 50 min, the yield of the final product decreases by 10-15%due to incomplete hydrolysis. With increasing time of incubation more than 60 min saturation of the drug pheromones are not increased because the hydrolysis process of this time is terminated and the flow of pheromones in the solution is terminated.

After carrying out all stages of the process the product is a dry porous mass, brownish hue with a specific smell. The shelf life of 24 months at a temperature not exceeding 20°C.

Before using the drug flamesword from aluminum cap and tube, dilute the contents of the vial in 100 ml of water at a temperature of 35-40°C, filtered through 2-3 layers of gauze filter and sprayed by sprinklers in places of detention of the sows at the level of the nasal mirror animals.

Example. To 1000 g of testes obtained from sexually Mature boars and cleaned of connective and adipose tissue were added 250 g of peeled celery root and 250 g of parsnip seed, crushed to versionrange state and homogenized in the homogenizer until smooth. In the resulting mass was made 15 g of enzyme protocolin and incubated in a thermostat at a temperature of 37-39°C for 60 minutes Then homogenized added 1515 ml of urine grunt, thoroughly mixed, and pressed using a press with cells of 0.7×0,7 mm the resulting liquid was poured into glass vials of 15 ml and subjected to sublimation. After the process of sublimation the product was a dry porous mass (0.5 g) brownish hue with a specific smell. Vials of the drug were closed with rubber stoppers and tried aluminum caps. Kept the drug at 20°C, the shelf life of 24 months.

Before use of the drug was dissolved in 100 ml of water at a temperature of 35-40°C and was filtered through a gauze filter.

Check drug sterility was performed using environments MPA and Endo with the following incubation in a thermostat. The toxicity of the drug was tested on 10 repair pigs of large white breed 5 months of age, which was placed in a special chamber machine and sprayed using an aerosol generator. During and after treatment replacement gilts manufactured drug natural sex pheromones grunt behavioral responses, the main clinical and hematological parameters were within physiological norms. In Guinea pigs (10 goals) after intra-abdominal injection (0.5 ml/bird) effects of toxicosis were observed, all experimental animals were alive.

The biological activity of manufactured drug was determined using a biological test and the use of special olfactometer. Compared manufactured drug drug prototype (including shelf life), urine sexually Mature boar, extract of celery root, extract of root of the parsnip seed, broth mixed with distilled water. When conducting Biotest completed ethogram, which included behavioral response in sows during contact with the test object. The results of Biotest showed (table 1), which sows the percentage of answers on the manufactured drug was higher (89.5 per cent)than in the drug-prototype (78,8%).

Production tests made ven the rata was performed in the conditions of teaching and experimental farm "Znamenskoye" Kursk state agricultural Academy named IVA, pig-breeding complexes "Magnetic" and "Timski" Kursk region.

The first scientific-production experiment was carried out repair immature pigs-analogues of large white breed 5 months of age. It was formed three groups of 35 animals. Pigs of group 1 were subjected to stimulation experienced by the drug. Pigs of group 2 were treated with drug-prototype. Pig 3 was the group's control, they were treated with distilled water.

Used drugs sex pheromones in a dose of 0.5 ml per animal by spraying a spray at the level of the nasal mirror, every other day for 20 days. Contained experimental and control animals in the same room and were fed the same diet. The sexual hunt the pigs was determined using grunt-probe.

The results of the first scientific-industrial experience has shown that the onset of puberty in pigs 1 experimental group occurred in 181,5±2,03-day age, the pigs 2 experimental group with 187,0±1.85 days, and the 3 pigs in the control group - with 205,5±2,11 days. Most pigs exposed stimulation pheromones, puberty occurred in the first 10 days after the start of stimulation, and the pigs of the control group, the onset of puberty was delayed and the 30-day observation period was manifested only in 16 (46%) animals. When the volume in pigs, which stimulated manufactured drug, the number of Mature animals was more - 31(89%) compared to animals, driven by drug-prototype - 28(80%) (table 2).

The second scientific-production experiment was carried out repair pigs analogous to 8 months of age, who had a good development, but puberty they did not come. From these pigs were selected three groups of 15 animals. Pigs 1 experimental group was treated with manufactured drug natural sex pheromone of the boar. Pigs 2 experimental group stimulated drug prototype. Pigs 3 group was the control and stimulation of the pheromone were not exposed. The pheromone stimulation was performed daily for 20 days, the dose was 0.5 ml/bird. Watched animals within 30 days, the sexual hunt was determined using grunt-probe.

The results of the second research and production experience showed that after stimulation produced by the drug in 11 (73%) pigs came puberty. When using drug-prototype puberty manifested in 9 (67%) pigs in the control group, 3 patients (20%) pigs (table 3.).

The third scientific-production experiment was carried out on lactating sows of large white breed. It was formed three groups of 30 sows each. Sows from the experimental group were subjected to the stimulation produced by the drug. Sows 2 experimental group stimulated drug prototype. Sow 3 groups were control and pheromones were not processed.

Stimulation of sows natural sex pheromones held for 5 days before weaning piglets and the subsequent 15 days daily. Dose was 0.5 ml/bird. When the manifestation of the estrous sows them artificially seminal. In the subsequent account of the number of farrowing sows and the number of received piglets.

The results of the third research and production experience has shown that within 20 days after weaning piglets among animals exposed stimulation produced by drug natural sex pheromone of the boar, was identified sexual hunting in 27(90%) sows. After stimulation drug prototype sexual hunting was observed in 25(83%) sows, and among the control animals in 19(63%). From sows 1 experimental group received more pigs (235 Gol.), than sows 2 (196 goal.) and 3 (144 goal.) groups (table 4).

Thus, the claimed method of producing drug natural sex pheromones grunt allows you to obtain the product with high biological activity and a longer shelf life.

Table 1
The results of biological testing of manufactured drug natural sex pheromone of the boar and drug-prototype
The analyzed objectThe results of Biotest (%)
Made medication
shelf life 12 monthsto 89.5
shelf life 24 months88,7
Drug-prototype
shelf life 12 months78,8
shelf life 24 months60,0
Urine sexually Mature boar68,5
The extract of celery root57,8
An extract from the root of the parsnip seed54,0
The broth feed40,0
Distilled waterthe 4.7

Table 2
The onset of puberty in replacement gilts subjected to stimulation by drugs natural sex pheromones grunt
GroupnCome puberty in pigs within days after stimulation
day
1-56-1011-1516-2021-2526-30Only
1 (manufactured product)35111055--31 (89%)
2 (drug-prototype)358863328 (80%)
3 (control)35-2 -55416 (46%)

Table 3
The onset of puberty in replacement gilts with delayed-puberty, subjected to stimulation by drugs natural sex pheromones grunt
GroupnCome puberty in pigs within days after stimulation
day
1-56-1011-1516-2021-2526-30Only
1 (manufactured product)15452---11 (73%)
2 (drug-prototype)154 4-1--9 (67%)
3 (control)15--2-1-3 (20%)

Table 4
The influence of the natural sex pheromone of the grunt at the onset of estrous sows and results of insemination
GroupnCame in the hunt for 20 days after weaning pigletsHe oporosis sowsReceived pigs
number%number%onlyFarrow
1 (manufactured product)3027 9024892359,8
2 (drug-prototype)30258320801969,8
3 (control)30196316841449,0

The method of producing drug natural sex pheromones grunt, including the crushing of testicles, their homogenization, addition of enzyme protocolin, incubation of 1.0-1.5% of the total weight of raw material, incubating for about 50-60 minutes at 37-39°C, centrifugation, mixing with urine in the ratio of 1.0:1.0 to 1.5, characterized in that the initial mass of the testes add celery root and the root of the parsnip seed in the ratio of 1.0:0.25 to:0,25 resulting mass after adding urine press in the press with cells of 0.7×0.7 mm, and the liquid sublimate.



 

Same patents:

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutics, and concerns a compositions for treating hair loss containing substantially i) a first alkyl ester of nicotinic acid with its alkyl fragment with a direct or branched chain containing 11 to 22 CH2 groups, and ii) a second alkyl ester of nicotinic acid with its alkyl fragment with a direct or branched chain containing 6 to 10 CH2 groups. The invention also concerns a method of treating hair loss in a subject, and also to a method of increasing thickness or "plenitude" of growing hair with using the offered compositions.

EFFECT: invention provides the compositions applied for hair follicle stimulation, exhibiting a favourable effect for treating hair growth disorders, such as alopecia, particularly in women.

8 cl, 2 dwg, 2 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: invention refers to cosmetics, namely to creation of new cosmetic face oil compositions encapsulated and presented in the form of softgel capsules that is the a single dosage form. The cosmetic face oil composition contains mixed vegetable oils, wt %: sesame oil 15-17, water-melon seed oil 9-13, walnut oil 8-10, peach oil 9-11, apricot oil 8-10, jojoba oil 2-3, lemon essence 2-3, olive oil - the rest to be encapsulated in the form of softgel capsules that is a single dosage form. This enables to increase shelf-life and retaining properties of the oils.

EFFECT: use of the offered composition provides rejuvenation and prevention of premature ageing, ability of skin to smoothing, active nourishment, eliminated desquamation, restored moisture-retaining power, intensified immune status of skin.

1 cl, 2 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to cosmetology and represents an ascorbic acid suspension in glycerin in which the ascorbic acid contents makes 13 wt % or more, a portion of ascorbic acid is dissolved in either glycerin, or glycerin containing diglycerin in the concentration 8 to 12 wt %, and a residue of ascorbic acid is deposited in the form of microcrystals of a particle diameter 25 mcm or less and uniformly dispersed in the given suspension.

EFFECT: invention provides improved skin sensation.

6 cl, 1 tbl, 3 ex, 6 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to cosmetology and represents an ascorbic acid suspension in glycerin in which the ascorbic acid contents makes 13 wt % or more, a portion of ascorbic acid is dissolved in either glycerin, or glycerin containing diglycerin in the concentration 8 to 12 wt %, and a residue of ascorbic acid is deposited in the form of microcrystals of a particle diameter 25 mcm or less and uniformly dispersed in the given suspension.

EFFECT: invention provides improved skin sensation.

6 cl, 1 tbl, 3 ex, 6 dwg

Silicon oxides // 2431465

FIELD: medicine, pharmaceutics.

SUBSTANCE: group of inventions refers to deposited amorphous silicon oxides with controlled abrasivity and effective cleaning properties for application in an oral care composition. There are offered amorphous deposited silicon oxide particles with an oil absorption value 85 cm3/100 g or less, an average particle diameter d50 3 mcm and less and d90 at which 90 wt % of particles have a diameter less than the value d90 6 mcm or less, with the value d99 at which 99 wt % particles have a diameter less than the value d99 10 mcm or less and at which a radioactive dentin abrasion value makes less than 150. There is offered a method of grinding amorphous silicon oxide involving fine grinding by jet, countercurrent jet, flat micronisation, or fluidised layer micronisation, and silicon oxide classification for formation of the amorphous deposited silicon oxide particles with the characteristics described above.

EFFECT: what is offered is application of said particles as an abrasive cleaning additive in an oral care composition, the oral care composition and a tooth-paste containing an acceptable carrier and an effective cleaning amount of said particles which clean teeth without surplus dentin or enamels abrasion.

19 cl, 7 tbl, 6 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: group of inventions refers to oral care compositions for dental erosion and/or abrasion control (i.e. prevention, inhibition and/or treatment). The offered tooth brushing composition for dental erosion control contains alkali fluoride as a fluoride ion source, an amphoteric surface-active material representing cocamidopropyl betaine, and a silicon dioxide dental abrasive as an abrasive. The tooth brushing agent have relative dentineabrasion (RDA) 20 to 60 and pH within 6.5 to 7.5 and does not contain a phosphate buffer, a water-soluble salt C10-18 alkylsulphate and calcium salt. A method of dental erosion and/or abrasion control involves application of an effective amount of said composition in a requiring individual.

EFFECT: use of said ingredients of the composition combined with RDA and pH control provides intensification of fluoride absorption by enamel, ability to protect against acid erosion stimuli, and to harden repeatedly enamel after an effect of such erosion stimuli.

13 cl, 8 tbl, 7 ex, 4 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: group of inventions refers to oral care compositions for dental erosion and/or abrasion control (i.e. prevention, inhibition and/or treatment). The offered tooth brushing composition for dental erosion control contains alkali fluoride as a fluoride ion source, an amphoteric surface-active material representing cocamidopropyl betaine, and a silicon dioxide dental abrasive as an abrasive. The tooth brushing agent have relative dentineabrasion (RDA) 20 to 60 and pH within 6.5 to 7.5 and does not contain a phosphate buffer, a water-soluble salt C10-18 alkylsulphate and calcium salt. A method of dental erosion and/or abrasion control involves application of an effective amount of said composition in a requiring individual.

EFFECT: use of said ingredients of the composition combined with RDA and pH control provides intensification of fluoride absorption by enamel, ability to protect against acid erosion stimuli, and to harden repeatedly enamel after an effect of such erosion stimuli.

13 cl, 8 tbl, 7 ex, 4 dwg

FIELD: medicine.

SUBSTANCE: present invention refers to liquid peroxide-containing oral care compositions containing a flavouring system which effectively masks undesired taste and sensations formed by peroxide. The liquid composition contains: (a) 0.01 wt % to 10 wt % of the composition, a pre-dissolved peroxide source chosen from hydrogen peroxide or urea peroxide; (b) 0.06 wt % to 0.095 wt % of the composition, a combination of the cooling agent menthol and at least the second cooling agent in which menthol makes 0.02 to 0.075 wt % of the composition, thus the second cooling is selected from: carboxamides, ketals, diols, menthol esters and their mixtures; (c) one or mixed components selected from group of certain flavouring chemical substances and suitable for the use in a mouth a liquid carrier. pH of the compositions is within 4 to 6.

EFFECT: composition provides a stable flavouring profile and steady freshening taste and sensation.

3 cl, 2 ex

FIELD: medicine.

SUBSTANCE: present invention refers to liquid peroxide-containing oral care compositions containing a flavouring system which effectively masks undesired taste and sensations formed by peroxide. The liquid composition contains: (a) 0.01 wt % to 10 wt % of the composition, a pre-dissolved peroxide source chosen from hydrogen peroxide or urea peroxide; (b) 0.06 wt % to 0.095 wt % of the composition, a combination of the cooling agent menthol and at least the second cooling agent in which menthol makes 0.02 to 0.075 wt % of the composition, thus the second cooling is selected from: carboxamides, ketals, diols, menthol esters and their mixtures; (c) one or mixed components selected from group of certain flavouring chemical substances and suitable for the use in a mouth a liquid carrier. pH of the compositions is within 4 to 6.

EFFECT: composition provides a stable flavouring profile and steady freshening taste and sensation.

3 cl, 2 ex

FIELD: medicine.

SUBSTANCE: present invention refers to liquid peroxide-containing oral care compositions containing a flavouring system which effectively masks undesired taste and sensations formed by peroxide. The liquid composition contains: (a) 0.01 wt % to 10 wt % of the composition, a pre-dissolved peroxide source chosen from hydrogen peroxide or urea peroxide; (b) 0.06 wt % to 0.095 wt % of the composition, a combination of the cooling agent menthol and at least the second cooling agent in which menthol makes 0.02 to 0.075 wt % of the composition, thus the second cooling is selected from: carboxamides, ketals, diols, menthol esters and their mixtures; (c) one or mixed components selected from group of certain flavouring chemical substances and suitable for the use in a mouth a liquid carrier. pH of the compositions is within 4 to 6.

EFFECT: composition provides a stable flavouring profile and steady freshening taste and sensation.

3 cl, 2 ex

FIELD: food industry.

SUBSTANCE: invention relates to a technology of deer farming products processing, in particular, to methods for production of biologically active products from complex raw material i.e. velvet antlers, tails and genitals. The method envisages grinding raw material i.e. maral buck velvet antlers, tails and genitals taken at a weight ratio of 60:20:20. The one performs extraction with 50% alcoholic solution acidated up to pH 4.7, the ratio of alcohol to raw material 7:1 during 12-14 days at a temperature of 20-25°C. One performs stirring for 5 minutes on a daily basis with subsequent extract freezing-out at a temperature of - 15 - (-18)°C during 5 days. Then the end product is filtered.

EFFECT: invention allows production of a highly effective finished product and more complete and rational usage of velvet antler deer raw material, in particular, maral tails and genitals.

6 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: invention refers to technology of biologically active agents (BAA) of animal origin. A method for preparing a BAA for prostate function recovery involves grinding bull and/or puberty bull-calf prostate tissue, homogenising the intermediate product, keeping the homogenate in 3% aqueous solution of acetic acid and zinc chloride (ZnCl2), separating water-soluble components of animal tissue cells by centrifugation to produce a supernatant to be concentrated, purified by ultrafiltration, adding glycine to the solution, sterilising and drying an end product. The solution of acetic acid is prepared of apyrogenic water; the homogenate is kept in the solution of acetic acid and zinc chloride at temperature 3-7°C for 48 h in being agitated by 0.5 portions every 4 h. The ultrafiltration process is executed in a circulation mode through a flat-chamber membrane installation with membrane filters of characteristic pore size d=300 A at temperature T≤10°C to produce a solution to be concentrated with the use of nano-filter compositional membranes. It enables to prepare an end product concentrate containing mixed ingredients of molecular weight M=5÷20.0 kDa to be dried by means of a spray-type drier.

EFFECT: method allows producing a high-yield powered therapeutically effective agent containing water-soluble ingredients of M<20,0 kDa.

4 cl, 1 ex, 2 dwg

FIELD: medicine.

SUBSTANCE: invention refers to surgery, and can be applied for treating trophic ulcers. It involves fenestration of a wound surface up to healthy, blood-supplied underlying tissue. It is followed with tangential excision of the wound surface. A complete wound hemostasis is carried out. Foetal allofibroblasts are injected in amount 1-7 million cells in a region of the fenestrated openings with irrigating a surface of trophic ulcer with a foetal allofibroblast solution. Autodermoplasty with a split-thickness sieve graft is performed.

EFFECT: method allows to stimulate granulation tissue growth in extensive injury.

2 ex, 4 dwg

FIELD: agriculture.

SUBSTANCE: invention relates to methods for transfer of embryonal stem cells to birds for production of gametes in them. Method consists in the fact that stem cells of rooster (ZZ) are introduced to hen bird in ovo. Specified bird is incubated until hatching. Specified hen bird is grown to pubescence with further reproduction of specified bird to produce mass of fertile bird eggs.

EFFECT: ratio of roosters to hens in eggs of this bird will be higher than without in ovo introduction of specified embryonal stem cells of rooster.

1 tbl, 15 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to gynecology, and deals with treatment of complicated forms of inflammatory diseases of uterine appendages. For this purpose, when performing abdominal operation from two sides between round ligament of the uterus and isthmic part of fallopian tube medicinal mixtures of the following medications are introduced: at first - lidase 16 U in 1 ml of 0.25% Novocain, after that without removing the needle prednisolone 15 mg, amikacin 0.25 mg in 1 ml of 0.25% Novocain. Drainage of small pelvis with gauze-glove pad on lateral pockets to retrouterine space and bed of remote mass is carried out. In post-operational period antimicrobial drug is introduced intravenously. Limphotropically from two sides from anterior commisure of large pudental lips introduced are medicinal mixtures of medications: first - lidase 16 U in 1 ml of 0.25% Novocain, after that without removing the needle - prednisolone 15 mg, amikacin 0.25 mg in 1 ml of 0.25% Novocain, slowly during 15 minutes. Such introduction is carried out 1 time a day for 7-8 days.

EFFECT: method ensures stimulation of interstitial humoral transport and lymphatic drainage and fast normalisation of clinical and laboratory parametres with reduction of course dose of antimicrobial drugs.

1 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to surgery and combustiology, and can be used in treating the patients with burn wounds. That is ensured by toilet. Then 1-2 days after trauma, the wound surface is covered with a bandage bottom of which is made of hydrophobic perforated silicone film "Carbosyl-P" coated with a layer of human collagen type I and human allogenic fibroblasts. As allogenic fibroblasts, characterised cells of human diploid fibroblast line M-22 at passage level №15-25 are used.

EFFECT: invention enables fast adequate epithelium repair over the wound at good functional and cosmetic result including in burns of II-IIIA degree, and also prevents development of local purulo-necrotic complications.

7 dwg, 3 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: invention concerns experimental medicine and osteoporosis therapy development. Method involves single injection of 0.5 ml of 10-day old rat embryo homogenate to femoral medullar channel of rat.

EFFECT: reduced treatment time due to improved osseous tissue structure faster tissue remodeling and prevented pathologic processes in tissue.

4 dwg

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely - to urology. On background of traditional treatment carried out is influence on prostate gland by chaotically changing magnetic field. Influence is rectal, with frequency 1 - 2000 Hz, strength 15-20 E. Duration of session 10-15 minutes. Preliminarily 1-2 hours earlier biologically active points selected from line E27, E28, E29, E30, R11, R12, R13, R14, VC2, VC3, VC4, V28, V31, V32, V33, V34, VG4 are influenced. Influence is performed by current of negative polarity, with intensity 50-100 mcA, voltage 9 V, after that suppository Vitaprost is introduced rectally.

EFFECT: method reduces time of treatment and increases remission duration.

2 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, particularly to gynaecology, and concerns chronic adnexitis treatment that is ensured by course of general hydrogen sulphide baths of temperature 36-37°, concentration of 50-140 mg/l, within 8-10 minutes, 6-8 baths every second day and gynaecologic irrigations at temperature 38°, concentration to 140 mg/l, within 10-12 minutes for course 6-8 daily procedures. Panties-like applications of sulphide silt mud are performed at temperature 38-42°, within 10-15 minutes every second day. Additionally, there are applied vulval pads with ointment Ephthypeloid U containing as follows, wt %,: diclofenac - 0.5, furasolidone - 0.5, metronidazole - 0.1, Lydasa PE - 2 PE, tetracycline - 0.8, erythromycin - 0.5, nystatin - 0.5 g 500000 units - 1.0, calendula extract - 0.3, chestnut extract - 0.3, dalargin - 0.2, Solbrol M - 0.2, Solbrol P - 0.06, Solbrol BR - 0.3, Extrapone V - 0.16, Eftillin - 40.0 and sulphide silt mud of Suksunsky pond - 55.0 in amount 2-2.5 ml for 6-7 hours daily within course 6-8 procedures.

EFFECT: method provides intensified and accelerated complex analgetic, antiinflammatory, antimicrobic action of Ephthypeloid U components and as consequence, normalisation of microcirculation and acceleration repair processes.

8 ex

FIELD: medicine.

SUBSTANCE: invention concerns medicine, in particular to gynecology, and concerns endometriosis treatments. With this goal glycoprotein is administered to the sick woman, allocated of a follicular liquid of cattle in a dose of 150-600 mg. Depending on degree of expression of an endometriosis from 10 to 30 injections of a preparation are carried out.

EFFECT: depression of risk of relapses, conservation of genital function.

2 cl, 3 ex

FIELD: agriculture.

SUBSTANCE: invention relates to the field of livestock farming. The method includes hormonal control of fertility in breeding by introduction of a synthetic gonadotrophin-releasing hormone-surphagon. Surphagon is introduced into an abdominal cavity of animal males as single injections in the amount of 0.4 mcg/kg for 5-10 days.

EFFECT: method provides for increased sexual reaction of animal males and higher fertility.

6 tbl, 1 ex

Up!