Method for prediction of rate of acute infectious diseases in infants
SUBSTANCE: method for prediction of rate of acute infectious diseases in infants with determining the immune status values on the first and fifth postnatal day: absolute lymphocyte count, differentiation marker carriers CD3, CD4, CD8, CD 19, CD56, CD95, a spontaneous nitroblue tetrazolium test value (NBTsp), a level of a phagocytic coefficient (PC), blood serum immunoglobulin A, M, G (IgA, IgM, IgG) concentrations, and further calculating a forecasting index (Ifor) with using a certain equation of multiple linear regression if Ifor does not exceed 3.2, a probability of no more than one acute infectious disease in an infant is concluded; if Ifor is within 3.2-3.7, 2 to 5 probable acute infectious diseases in an infant is stated; if Ifor exceeds 3.7, probability of more than 5 acute infectious diseases in an infant is concluded.
EFFECT: higher prediction accuracy.
The invention relates to medicine, namely to neonatology, and can be used to determine the effect of the immune status of the first and fifth days of life on the development of morbidity in children during the 1st year of life.
Known "Method of predicting the state of the immune system of the newborn" (patent No. 2107912, priority from 26.09.1994).
The authors provide an early prediction of the immune system of the newborn. In umbilical cord blood immediately after birth to determine the relative content of b-lymphocytes and indices greater than or equal to 18%, forecast immunodeficiency condition.
The disadvantages of this method are: identify indicators only humoral immunity, evaluate the relative amount of b-lymphocytes, and in the neonatal period most appropriate to assess the total amount. Investigate cord blood once without taking into account the dynamics.
Known "Method of early diagnosis of abnormalities in the immune status of the newborn" (Patent No. 92001927, priority from 23.10.1992).
Examine a sample of blood of a newborn child and determine it diagnostically significant factor, at the same time explore a sample of umbilical cord blood, which as diagnostically significant indicators determine the migration index of leukocytes and levels of immunoglobulin m and M and the value of the migration index of leukocytes less than 45% or more than 100%, and the level of immunoglobulin A - more than 0.08 g/l diagnosed early deviation in the immune status of full-term newborn baby. The presence of abnormalities in the immune status was confirmed by the frequent development of acute respiratory bacterial viral infections and the development of allergic diathesis in children in the first year of life.
The disadvantages of this method are: prognostic criteria are given without taking into account the dynamics of changes in parameters.
Known dissertation research Karpova NV "immune and cytokine systems are often ill children and methods of correction of violations" (2008).
The paper presents a comprehensive survey of the FIC on the indicators of the number of subpopulations of lymphocytes, the level of immunoglobulins in the serum, interferonogenesis (biological tests and enzyme-linked immunosorbent assay), serum content and production of several cytokines. It is revealed that the immune and cytokine status healthy and sickly children of the same age differ. These differences lie in the reduction of the FIC with the largest subpopulations of lymphocytes, induced production of γ, spontaneous products α, the ratio T×1/T×2 and the increase induced production IL. We found a decrease in the number of subpopulations had no effect on the occurrence of acute respiratory disease during the observation FIC, but reducing the induced and γ, as well as the ratio of production of IFN T×1/T×2 contributed to the occurrence of ARI in the same period. The concentration of immunoglobulins in the serum FIC was changed in comparison with healthy children as follows - IgG was slightly reduced, IgM remained within the normal range, the concentration of IgA in most children was at the lower limit age limit, therefore, the function of b-lymphocytes in FIC is somewhat reduced. The readiness of the organism to respond adequately to emerging infection depends in particular on the balance between T-helper type 1 and 2. In the present work, the state of this balance was estimated by the ratio of cells (T×1) or IL (T×2), and was designated as producing IFN T×1/T×2. It turned out that the FIC this ratio is lower than in healthy children. These data confirm the widespread opinion that the relative predominance of T×2-immune response may be associated with deterioration of immune protection against viral and bacterial infections. The FIC reduced spontaneous products α and increased induced production IL. All of the above data on the status of the immune and cytokine systems in FIC indicate that FIC a number of changes in the indices of cellular and humoral immunity.
The disadvantages of this method are: the evaluation system contains a large number of what about the components, when this clear criteria for distinguishing between the probability of incidence is increasing missing.
A known method for predicting the state of health of children 1 year of life (Ibershoff, Tveria, Whitefence, Evernote, SENCHENKOV. The problem is sickly and children and optimization methods of their treatment / Journal "child Health", 2(11) 2008) determination of lysozyme and secret IgA in saliva and bactericidal activity of the skin, since the neonatal period. By reducing the content of lysozyme, IgA, and bactericidal activity of the skin from the level typical for children of group I health, predicted the development of frequent sickness in 80% of cases.
The disadvantages of the method: the proposed prognostic criteria are used to assess the local protection of the body; prognostic criteria are given without taking into account the dynamics of changes of indicators in the 1st year of life.
The technical result of the proposed method - determination of the influence of immune status of the first and fifth days of life on the development of morbidity in children during the 1st year of life, allowing time to assign corrective therapy and reduce the risk of disease development.
The technical result is achieved through examination of 51 child from the period of birth to 2 years of life.
The study of the immune status held the 1-2 and 4-5 days of life, because by this time of the end of the acute period of adaptation of the child to the conditions of extrauterine existence. Defined subpopulation structure of lymphocytes, media differentiation markers CD3, CD4, CD8, CD19, CD56, CD95 NSDP, phagocytic index, the concentration of IgA, IgM, IgG.
We studied the continued development of these children (from birth to 1 year). In the study of follow-up monitoring were assessed: physical development, psychological development, somatic morbidity and degree of resistance. The degree of resistance was determined by the ratio of acute illnesses suffered by the child during the year.
It is established that the frequency of acute respiratory viral diseases depends on immunological parameters in children in the first and fifth day after birth.
To clarify the criterion for predicting the frequency of occurrence of diseases in the first year of life was used the method of multiple linear regression, where the dependent parameter were made by the frequency of occurrence of acute infectious diseases in the first year of a child's life, as the adjustable parameters are indicators of the immune system on the first and fifth day after birth, in particular the absolute number of lymphocytes, media differentiation markers CD3, CD4, CD8, CD19, CD56, CD95 and the rate of spontaneous test of nitrosonium tetrazolium NSDP), level phagocytic index (AF)classes of immunoglobulins a, M, G (IgA, IgM, IgG).
The regression equation has the following form:
Index forecasting the incidence of children in the first year of life = -4,49057-0,115041·CD3_1+0,117647·CD4_1+0,148533·CD8_1-0,0078096·CD19_1+0,000470342·CD56_1-0,010362·CD95_1-0,0438747·STSP+0,0131639·FP-0,345831·IgA_1-0,0782763·IgM_1+0,0470971·IgG_1+0,0336856·CD3_5-0,000220081·CD4_5-0,0199284·CD8_5+0,059304·CD19_5+0,070623·CD56_5+0,0229516·CD95_5+0,0526357·STSP+0,0157991·FP+0,775039·IgA_5+0,117594·IgM_5-0,0111648·IgG_5.
CD3_1 - the absolute number of lymphocytes, media marker of differentiation of CD3 on the first day after birth;
CD4_1 - the absolute number of lymphocytes, media marker of differentiation of CD4 on the first day after birth;
CD8_1 - the absolute number of lymphocytes, media marker of differentiation of CD8 on the first day after birth;
CD19_1 - the absolute number of lymphocytes, media differentiation marker CD19 on the first day after birth;
CD56_1 - the absolute number of lymphocytes, media differentiation marker CD56 on the first day after birth;
CD95_1 - the absolute number of lymphocytes, media differentiation marker CD95 on the first day after birth;
NSDP is an indicator of spontaneous test of nitrazine tetrazolium on the first day after birth;
FP - phagocytic index on the first day after birth;
IgA_1 level of immunoglobulin class And on the first day of polerowanie;
IgM_1 level of immunoglobulin M class on the first day after birth;
IgG_1 level of immunoglobulin G class on the first day after birth;
With paetkau the same indicators on the fifth day after birth.
Using the training and control samples found that children who had no more than one disease during the first year of life Index forecasting the incidence of children in the first year of life (IPR) did not exceed a value of 3.2; in children undergoing from 2 to 5 diseases, Ypres was in the range of 3.2 to 3.7; sickly children who have had more than 5 acute infectious diseases in the first year of life, the index has exceeded the value of 3.7.
Thus, the use of the proposed method to assess the risk of incidence of acute infectious diseases in the first year of a child's life and timely aimed correcting therapy, contributing to the overall resistance of the organism.
As materials, further justifying its contained extracts from the history of the development and history of disease of the newborn.
Example 1. Girl HD, 2009 birth, 1 year.
Clinical diagnosis in the neonatal period: Respiratory distress syndrome 3 degrees (m aspiration meconium plug waters). Aspiration pneumonia. Cerebral ischemia 3 degrees, OST the initial period. Convulsive syndrome. Hypertensive syndrome. The syndrome of multiple organ failure (cardiac, pulmonary, renal, adrenal, hepatic). Natal trauma AIZ (dislocation of the C2-C3).
The baby was born on the 1st of pregnancy, occurring against the background of the combined preeclampsia moderate severity (edematous syndrome), on the background of bronchial asthma, the threat of termination, from an early time (4 weeks) received Duphaston to 28 weeks of pregnancy. The OAS (infertility for 4 years, NMC, the threat of cancer), chronic Feto-placental insufficiency, chronic intrauterine hypoxia. Medical history: acute respiratory infections (2 times per year), bronchial asthma since 2002.
Birth 1, in the period 39-40 weeks, 1 p-d 15 h 45 min, 2 p-d 15 minutes Epidural anesthesia. Waterless period of 5 hours 30 minutes, the fluid is green, moderate quantity.
Body weight at birth 3500 g, body length 52 cm, head circumference 36 cm, chest circumference 33 see Estimation on Apgar scale 3-6 points. The child was born in severe asphyxia. RD 3 degree (meconium plug aspiration).
The child was seen as difficult due to severe asphyxia, aspiration meconium plug waters, respiratory disorders (score from Downes - 4 points). It was noted moaning breath, with the participation of accessory muscles, moist rales are heard in the basal regions, marked W the position of the diaphragm during breathing, breathing weakened. Given the inefficiency of spontaneous breathing, increase respiratory disorders: dyspnea, indrawing of the intercostal spaces, the retraction of the sternum, the child was transferred to the IVL device SLE-2000 parameters FiO21,0; Pip 28; Peep 2; f 66; J:E=1:1,3 with the operation of the apparatus synchronizes in the background of sedation and myorelaxation. Thorax symmetric excursion sufficient. Breathing hard, is all of the fields, heard traiterous and moist rales. BH 65 minutes When sanitizing of the endotracheal tube saniyede rich yellow-green mucus. From the stomach saniyede bright slime.
Warm under a lamp radiant heat. The condition is very severe. Muscular hypotonia, hyporeflexia. On the background of the General depression of the Central nervous system have any bouts clinico-tonic convulsions. Skin dyed green water, light diffuse cyanosis. Symptom pale spots <5 sec. General pastos tissues.
In General blood analysis: Hb 183 g/l, at the hands of Eritrean. 5,0·1012/l, leukocytosis 9·109/l, trom. 291·109/l, p/poison 4%, poison 51%, lymphocytes 21%, mo - 7%, as 1%, retic 15‰.
In urinalysis: protein - 0,81 g/l, lake. - 2-4 p/SP, at the hands of Eritrean. - 5-8 p/SP, glucose neg, acetone - OTP reaction 5.5 pH, uric acid - a lot.
In the biochemical analysis of blood: public bilir - 105,0 μm/l, glucose 5.0 mm/l, Alat amounts to 188.7 nmol/CL, AST 181,2 nmol/SL, much the wine 11.8 mm/l, total protein of 53.7 g/l CRP (+).
Radiography of the chest in a horizontal position, the lung field is reduced transparency, enhanced pulmonary figure, traced focal shadows punctulata, different intensity more to the right. The sinuses are free. The mediastinal shadow is not changed. Conclusion: x-ray signs of aspiration syndrome.
Radiography of the chest in the dynamics with the capture of the abdominal cavity, speaker negative to the right and in the medial division of pulmonary transparency drastically reduced with indistinct contours. Pulmonary pattern is reinforced. The sinuses can be traced. Heart expanded in diameter. Abdomen almost airless, single gas accumulation to the left of the spine at the level of L2-L5. Conclusion: signs of aspiration pneumonia.
Immunological study of peripheral blood on the 1st and 5th day of life showed a change in immunological parameters that indicate the violation of the processes of immunoregulation and lead to disruption of the mechanisms of adaptation in neonates and start the development of pathological reactions, manifested clinical signs of infection.
Treatment: mechanical ventilation, infusion therapy, dehydration therapy, electrolytes, cardiotonic, muscle relaxants, nootropics, anticonvulsants, obezbolivaushee therapy, editiona, muscle relaxants, antibiotics, biologics, diuretic, fresh frozen plasma.
On the 5th day. transferred to the RCC in ı1.
During the follow-up observation, it was found that in the first year of life the child 4 times suffered from an acute respiratory viral infection, acute obstructive bronchitis, pneumonia. Once a year ill with acute intestinal infection unspecified etiology type gastroenterocolitis moderate severity, infection of the urinary tract.
At the age of 8 months the child had atopic dermatitis.
During the first year of life the girl had repeatedly been treated in ı1 and received appropriate treatment: infusion therapy, nootropics, anticonvulsant therapy, hormonal therapy, antibacterial therapy, dehydration therapy, biologics, massage, paraffin bath, etc. together with immunomodulating therapy not received, i.e. immunotherapy was not conducted.
As it was not taken into account the index predict the incidence of acute illnesses, the child did not receive, respectively, together with immunomodulating therapy. The result is that the child belongs to the group of sickly and children and has a low degree of resistance. Subsequent calculations showed that the index forecasting the incidence of acute infectious diseases in the PE the first year of life this child was Ypres=3,65 - the forecast from 2 to 5 diseases during the first year of life.
Example 2. Boy S.A., 2009, year of birth, 1 year.
Clinical diagnosis in the neonatal period: Cerebral ischemia 2 degrees, the acute period. CNS depression syndrome. RD 2 degrees. Edematous syndrome. The IUGR 1 degree gipotermicescomu option. Transient hyperbilirubinemia. Intubation of the trachea. IVL. Catheterization of the umbilical vein. High risk of infectious diseases. The symptom of the failure.
The baby was born on the 2nd of pregnancy, occurring against the background of exacerbation of chronic pyelonephritis, combined preeclampsia (edematous syndrome) moderate severity, threatened miscarriage, chronic Feto-placental insufficiency, chronic intrauterine hypoxia. The OAS (the scar on the uterus).
Medical history of the mother during pregnancy SARS with increasing temperature up to 38°C. In childhood chickenpox, rubella. Chronic pyelonephritis in 1994, exacerbation during pregnancy.
Birth 2nd, urgent, pathological. Birth by emergency caesarean section. B/in the period of 0 hours, amniotic fluid stained with blood, and a moderate quantity. Abruptio low located placenta. The bleeding. Acute fetal hypoxia.
Body weight at birth 2820 g, body length of 50 cm, head circumference 33 cm, chest circumference 33 see Estimation on Apgar scale -6 points. The child was born in severe asphyxia. RD 2 degree (edematous syndrome).
In the genus. the hall was reorganize the SDT, vvl bag "Penlon" 100% oxygen through a mask within 5 minutes Of the race. hall child immediately transferred to the ER and ITN.
The child was seen as difficult due to severe asphyxia, RDS 2 degree (edematous syndrome). Given the above, the child is transferred to NCPAP apparatus SLE-2000, the starting parameters FiO2- 0,5%, Rear +5 cm H2O. Is under the lamp suchitoto heat. Distress not growing. The child periodically podstegivaet, on the lips scarce frothy discharge. Given the lack of a "+" effect parameters NCPAP changed FiO2- 0.6%, Rear +6 cm H2O, there is a positive effect. Not moaning. Course NCPAP is completed, the child is transferred to the DCT (FiO20,45 4-6 l/min). Outside grants oxygen, the condition worsens, increasing shortness of breath up to 80 min, marked diffuse cyanosis. Chest of podstata laterally, the breath is held from 2 sides with the participation of accessory muscles, indrawing of the diaphragm, the intercostal spaces, traiterous rales in all fields. BH 64 minutes Score from Dowries 3 points. Warm under a lamp radiant heat. The condition is very severe. Muscular hypotonia. Hyporeflexia. Locomotor activity was reduced. Tremor of the limbs. The skin is clean, light diffuse cyanosis. The symptom is ledno spots" < 5 sec. General pastos tissues. Given the growth of respiratory failure, critical condition, after a preliminary rehabilitation LDP and the SDT, as well as sedation of 0.5% Sol. Sibazoni 0.5 ml/m, produced tracheal intubation tube "Portex" No. 3 on the depth 9,0 see Started IVL device Bear cub 750 psv settings FiO20,6; Pip 31; Peep 3; f 72; J:E=1:1,2 with the operation of the apparatus synchronizes in the background of sedation and myorelaxation. Thorax symmetric excursion sufficient. Breathing in the basal regions are heard better, at the height of inspiration can be heard traiterous wheezing. When sanitizing of the endotracheal tube saniyede abundant bright mucus. From the stomach saniyede bright slime.
In General blood analysis: Hb 181 g/l, at the hands of Eritrean. 4,8·1012/l, leukocytosis 8,7·109/l, p/poison 3%, poison 65%, n - 1%, lymphocytes 20%, mo - 8%, as 2%, retic 35‰.
In urinalysis: protein - 2,122 g/l, lake. - 8-10 p/SP, at the hands of Eritrean. - 5-8 p/SP, glucose neg, acetone - OTP, the reaction of 5,5 pH, salts of phosphates are many, drag. mushrooms found.
In the biochemical analysis of blood: public bilir - 64,0 μm/l, tie. the 4.65 µm/l, glucose 6 mmol/l, Alat 105 nmol/CL, AST 128 nmol/CL, urea 8.2 mm/l, total protein 40.6 g/l, CRP (-).
Radiography of the chest from 13.07.09. in the horizontal position, the lung fields are symmetrical pneumat., moderately thickened pulmonary drawing by interstice. Roots structureless. Traced the airbag is I bronchogram. Sinuses free, no configuration, enlarged mediastinum. Conclusion: x-ray signs of edema syndrome.
Immunological study of peripheral blood on the 1st and 5th day of life showed a change in immunological parameters that indicate the violation of the processes of immunoregulation and lead to disruption of the mechanisms of adaptation in neonates and start the development of pathological reactions, manifested clinical signs of infection.
Index predict the incidence of acute illnesses Ypres=4,09014
Treatment: mechanical ventilation, infusion therapy, dehydration therapy, electrolytes, cardiotonic, muscle relaxants, nootropics, anticonvulsants, obezbolivaushee therapy, sedatives, muscle relaxants, antibiotics, biologics, diuretic.
At the age of 4 days transferred to the RCC ı1.
During the follow-up observation, it was found that in the first year of life the child 2 times suffered from an acute respiratory viral infection, complicated by obstructive bronchitis. Once a year ill with acute intestinal infection unspecified etiology type gastroenterocolitis moderate severity.
At the age of 5 and 11 months had atopic dermatitis.
According to neurosonography aged 1 month - diffuse changes in hypoxic-ischemic in nature, risaki slight dilatation of the interhemispheric fissure.
The boy is in the dispensary with a neurologist about perinatal encephalopathy. During the 1st year of life physical development above average harmonious, delayed motor development.
During the 1st year of life the child received appropriate treatment: infusion therapy, nootropics, anti-bacterial therapy, dehydration therapy, biologics, massage, paraffin bath.
With regard to the definition by using the proposed index is predictive of the risk of high morbidity in the first year of life the child is assigned to immunomodulatory therapy.
In the genus. house:/drip immunoglobulins 4 ml/kg of body weight per day, in front of/with the introduction diluted with 0.9% sodium chloride or 5% glucose in the ratio 1:4, enter/infusion at a rate of 8-10 drops per minute. The course is 5 days.
In the clinic: KIP - Feron suppositories in a daily dose of 500,000 IU (1 suppos.), rectal, 1-2 times a day, the course of treatment is 7-10 days. Suppositories administered rectally after cleansing enema or bowel movements. When intestinal infections Kipferon suppositories administered in an average dose of ME 50000 per 1 kg of body weight, but not more than 1 million IU/day (2 suppos.) The course of treatment is 7 days.
Anaferon prophylactically in 1 tab. 3 mg, administered 1 tablet. in day. The rate of 30-40 days.
Viferon 150000 IU - 1 suppository daily for 1 suppositor and 2 times per day after 12 h rectally. The course of treatment is 5 days.
According to the index predict the incidence of acute illnesses the child should be referred to the group with the risk of more than 6 diseases in the year, however, the timely use of immunomodulatory therapy on the basis of accounting Ypres were able to transfer it to a group with fewer diseases. According to the observations in the first year of life the child does not belong to the group sickly and children, harmoniously developed and has a high degree of resistance.
A method for predicting the incidence of children with acute infectious diseases in the first year of life with determination on the first and fifth day after birth indicators of immune status: the absolute number of lymphocytes, media differentiation markers CD3, CD4, CD8, CD19, CD56, CD95, the rate of spontaneous test of nitrosonium tetrazolium (NSDP), level phagocytic index (FIS)concentrations in serum immunoglobulin classes a, M, G (IgA, IgM, IgG), characterized in that the index of prediction (IPR) is determined using equation of multiple linear regression:
where mark _1 indicates on azatel on the first day after birth, mark _5 indicator on the fifth day after birth;
if the IDP does not exceed 3,2 - judgments about the probability of moving in the first year of life no more than one acute infectious diseases; if the IPR is in the range of 3.2 to 3.7 - likely to occur from 2 to 5 acute infectious diseases in the first year of life; if the IPR is greater than the value of 3,7 - judgments about the probability of occurrence of more than 5 acute infectious diseases in the first year of life.
SUBSTANCE: complete blood count is performed, and the leukogram components are used to calculate integral hematological values: Calph-Caliph leucocytic intoxication index - LII1, Ostrovsky leucocytic intoxication index - LII2, stress index - SI, neutrophil-lymphocyte index - NLI, neutrophil-monocyte index - NMI, lymphocyte-monocyte index - LMI. If observing the values LII1≤2.5, LII2≤2.75, SI≤1.0, NLI≤11.0, NMI≤16.0, LMI≤2.0, a mild severity is detected. The values LII1 2.6 to 4.5, LII2 2.76 to 5.0, SI 1.1 to 2.0, NLI 11.1 to 15.0, NMI 16.1 to 24.0, LMI 2.1 to 2.5 enables to predict a moderate severity level. A severe level is ensured by LII1> 4.5, LII2> 5.0, SI> 2.0, NLI> 15.0, NMI> 24.0, LMI> 2.5.
EFFECT: use of the declared method allows higher objectivity of determining severity level of acute pancreatitis.
4 ex, 2 tbl
SUBSTANCE: method of early solid malignant disease staging (at the stage of provisional clinical diagnosis) by cell number with stable ontogenetic disorders detected in patient's peripheral blood lymphocytes by quantitative and qualitative analysis of stable chromosomal and genomic disorders in metaphase plates from patient's peripheral blood lymphocytes.
EFFECT: method enables following disease change shift in response to treatment and determining clinical effectiveness.
5 ex, 1 tbl
SUBSTANCE: method of diagnosing rejection of kidney allotransplant includes determination of phase height PH of peripheral blood live lymphocytes by method of phase-interference microscopy, determination of quantity of lymphocytes with phase height PH≤1.5 mcm, 1.5 mcm<PH≤2 mcm, 2 mcm<PH≤2.5 mcm, PH>2.5 mcm, selection of lymphocyte activity coefficients for each limit, said phase heights of lymphocytes equal k3=3, k2=2, k1=1, k0=0 respectively. Obtained data are used to determine functional activity of lymphocytes in sample by formula: FA=(k3n3+k2n2+k1n1+k0n0)/n, where n is number of lymphocytes in sample, n3 is number of lymphocytes with PH≤1.5 mcm, n2 is number of lymphocytes with 1.5 mcm<PH≤2 mcm, n1 is number of lymphocytes with 2 mcm<PH≤2.5 mcm, n0 is number of lymphocytes with PH>2.5 mcm, k3, k2, k1, k0 are coefficients of lymphocyte activity, and if value of lymphocyte functional activity is within FA=1.8-2.0, rejection of kidney transplant is diagnosed.
EFFECT: application of claimed method makes it possible to set diagnosis in due time, which considerably increases efficiency of anti-crisis therapy in post-transplantation period.
2 ex, 1 tbl
SUBSTANCE: claimed is method of diagnosing antioxidant function of membranes of erythrocytes in case of exacerbation of herpes-viral infection. Method lies in determination of superoxide dismutase concentration and titre of antibodies to herpes virus by method of immunosorbent assay (ELISA) of peripheral blood in pregnant women after exacerbation of herpes-virus infection. If superoxide dismutase concentration is 297.63±13.16 Unit/gHb (control - 386.77±13.12 Unit/gHb), titre of antibodies to herpes virus in peripheral blood of pregnant woman constituted 1:3200. If titre of antibodies is 1:6400, superoxide dismutase concentration decreased to 243.85±7.71 Unit/gHb (control - 386.77±13.12 Unit/gHb; p<0.001).
EFFECT: reduction of superoxide dismutase concentration in erythrocytes of peripheral blood in pregnant women during exacerbation of herpes-virus infection predicts reduction of protective reaction of toxic impact of lipid peroxidation on membranes of erythrocytes, increasing risk of their destruction.
SUBSTANCE: essence of method of predicting character of progressing course of chronic kidney disease (CKD) lies in determination of blood coagulation characteristics: activated partial thromboplastin time (aPTT), prothrombin time (PT), thrombin time (TT), antithrombin III (ATIII) level, von Willebrand factor (WF), endothelin-1 (E-1), D-dimer. If aPTT indices are within the range from 28 to 35 seconds, PT is from 12 to 15 seconds, TT is from 9 to 13 seconds, ATIII level is from 29 to 70%, WF level is from 105 to 115%, E-1 does nor exceed 0.32 fmol/ml and D-dimer is from 245 to 520 ng/ml FEU slow progressing of CKD is predicted. If aPTT, PT, TT, ATIII level are the same, and WF is higher than 115%, E-1 is higher than 0.32 fmol/ml, D-dimer is higher than 620 ng/ml FEU, accelerated CKD progressing is predicted.
EFFECT: increase of accuracy and reliability of chronic kidney disease diagnostics.
2 ex, 2 tbl
SUBSTANCE: method includes determination of ceruloplasmin level on blood plasma. If its values are from 202.8 to 266.8 mg/l, thymus weight is higher than 0.2 g and content of lymphoblasts in it is more than 19%, development of re-inoculated lymphoblastic leukemia in line AKR/JY mice is diagnosed.
EFFECT: method makes it possible to diagnose syngenic re-inoculated lymphoblastic leukemia in mice quickly and accurately.
1 tbl, 1 ex
SUBSTANCE: coagulation profile parameters are evaluated: fibrinolytic activity (FA), activated partial thromboplastin time (APTT), fibrin split products (D-Dimers) and international normalised ratio (INR). It is followed by calculating an integral coagulation factor (ICF) by formula: ICF=0.06FA+0.25INR+0.08 D-Dimer+0.54 APTT+6.54 where ICF is integral coagulation factor, FA is fibrinolytic activity (min.), INR is international normalised ratio (unit), D-Dimer is fibrin split products (ng/ml), APTT is activated partial thromboplastin time (sec.). The value ICF≥1 refers to a high risk, while the value ICF<1 - to a low risk.
EFFECT: application of the method enables evaluating a degree of risk of gestational thrombogenic complications that makes it possible to plan and control therapeutic actions aimed to normalise blood circulation in the mother-placenta-foetus system.
SUBSTANCE: in a cell containing an agitated blood sample, a primary measuring channel is formed by means of a collimated narrow optical beam. Then a photoconverter is used to register light intensity through the primary and to isolate thereafter constant and variable electric signal components, and to determine a mean value of thrombocyte aggregates. Immediately before completion of sample agitation, a mean ICK light intensity through termination the primary measuring channel formed in the cell at the bottom of the sample. On completion of agitation, an additional measuring channel similar to the primary one is formed at the top of the sample near to its level wherein time-stable light intensity value Iy is registered. A logarithm of the relation Iy/ICK shows a degree of aggregation.
EFFECT: invention enables measurement of the same sample both of mean aggregate value, and the degree of thrombocyte aggregation, and more accurate evaluation of these characteristics.
3 cl, 5 dwg
SUBSTANCE: pressure equal to existing capillary pressure required to pass the entire red cell suspension by micro-sieving through a calibrated filter is generated in a simulated medium. It involves filtration time recording of the analysed blood by a stop watch. Passage time of the analysed red cell suspension shows a red cell deformability index - RCDI which is calculated by formulae: and where Xi is an average filtration time, n is number of observations.
EFFECT: use of the method allows quick and precise quantitative analysis of red cell deformability.
1 tbl, 3 ex
SUBSTANCE: for thrombocyte aggregation analysis, a blood sample is mixed by periodic absorption of a sample portion from a container into a capillary to be thereafter displaced to the container back. An electric signal is transformed into light beam intensity passed through a segment of the capillary found in a measuring channel and relevant to double crossing of said segment by a meniscus during each aspiration and displacement cycle in the electric signal. The pulses formed by crossing of the light beam by the sample during each aspiration and displacement cycle are isolated. An average value of the amplitude Uc of each pulse is related to a component level of the electric signal preceding its rise-up portion; its root mean square deviation σ is calculated, while the mean size of thrombocyte aggregates is shown by the squared relation σ/Uc.
EFFECT: more uniform mixing of small volume of the blood samples and eliminated action of optical transmission of the capillary walls and light beam instability on the measurement results.
4 cl, 6 dwg
SUBSTANCE: invention relates to laboratory methods for blood analysis. Plasma is dropped in copper sulfate solution with density 1.023 g/cm3, not above, and time for drop falling on bottom of graduated cylinder with column height 243 mm is measured. The blood plasma density value is calculated by the formula:
wherein is the unknown blood plasma density (g/cm3); is copper sulfate solution density measured by areometer (g/cm3); t is average falling time of plasma drop in the copper sulfate solution (as seconds); 0.260130126 and 0.00290695 are correction coefficients. Temperature of plasma and copper sulfate solution is 20oC. Method is simple and suitable and allows carrying out analysis of small volumes of blood plasma and to reduce analysis time.
EFFECT: improved assay method.
SUBSTANCE: method involves carrying out microscopic examination of blood serum samples taken from femoral vein and cubital vein. Femoral vein sample is taken on injured side. The examination is carried out before and after treatment. The blood serum samples are placed on fat-free glass slide in the amount of 0.01-0.02 ml as drops, dried at 18-30°C for 18-24 h. The set of pathological symptoms becoming larger or not changed after the treatment in comparison to sample taken before treatment, and morphological picture of samples under comparison taken from the cubital vein showing no changes or being changed to worse, the treatment is considered to be effective.
EFFECT: enabled medicamentous treatment evaluation in course of treatment to allow the treatment mode to be changed in due time; avoided surgical intervention (amputation); retained active life-style of aged patients.
FIELD: medicine, obstetrics, gynecology.
SUBSTANCE: in the first trimester of pregnancy one should study the content of CD8+CD11b lymphocytes and at their values being either equal or above 2% it is possible to predict gestosis. The present innovation enables to choose correct tactics of treating pregnant women that, in its turn, leads to decreased frequency of this complication of pregnancy and the risk for the development of fetal and neonatal pathology.
EFFECT: higher accuracy of prediction.
3 ex, 1 tbl
SUBSTANCE: the present innovation deals with studying and treating diseases of inflammatory, autoimmune and degenerative genesis. One should perform sampling of heparinized blood followed by its sedimentation to obtain blood plasma with leukocytes and centrifuging to isolate the latter which are washed against erythrocytic and serumal admixtures, and, also, it deals with calculating the number of cells in samples out of leukocytic suspension after incubation (B) for 1.5 h at 37 C in holes of plastic microplotting board, out of leukocytic suspension one should additionally prepare two samples, one should be applied to calculate total number of leukocytes before incubation (A), the second sample undergoes incubation at the same mode at addition of autoserum to calculate the number of cells remained after incubation (C). One should state upon adhesive properties of leukocytes by the index of spontaneous adhesion (D), where D=(A-B)/B.100%, and effect for enhanced cellular adhesion under the impact of autoserum should be detected by the value of K=(B-C)/C.100% at K ≥ 30%, where B - C - the number of cells undergone additional adhesion after addition of autoserum. The present innovation widens functional possibilities of the suggested method due to obtaining additional values depicting adhesive properties of blood leukocytes.
EFFECT: higher accuracy of detection.
FIELD: medicine, diagnostics.
SUBSTANCE: the present innovation deals with blood sampling, separating plasma against erythrocytes, moreover, in plasma on should detect activity of antithrombin III, proteins C and S, XIIa-dependent fibrinolysis and concentration of plasminogen obtained results should be expressed as relative units followed by calculating integral parameter that characterizes the state of anticoagulant-fibrinolytic potential (IPAFP) by the following formula: IPAFP = [(C1 + C2)/(C3 + C4)] x 100, where C1 - the ratio of observed value of antithrombin III activity to the value of inferior border of the range of analogous parameter norm; C2 - the ratio of observed value for the activity of proteins C and S system to the value of inferior border of the range of this parameter norm; C3 - the ratio of the value of inferior border of plasminogen concentration under normal conditions to observed value of analyzed parameter; C4 - coefficient calculated with the help of regression equation: C4 = 0.9 + (0.01 x X), where X - terms of lysis of patient's euglobulin clot/min, and at IPAFP value of 101.4 U and higher one should state anticoagulant-fibrinolytic blood potential to be in norm, in interval of 64.8 - 101.3 -as insufficient, and at 64.7 and below - as critical. The present method simplifies the procedure of evaluating the state of endogenous anticoagulants and activity of XIIa-dependent fibrinolysis.
EFFECT: increased diagnostic value of obtained results.
3 ex, 1 tbl
FIELD: medicine, laboratory diagnosis.
SUBSTANCE: method involves determination of the patient blood content of globulin-alpha 1, globulin-beta, globulin-gamma and the total bilirubin content followed by calculation of diagnosis indices for the patient (Y1, Y2, Y3) by using the computer program "Statistica 1.5" and introducing values X1, X2, X3 and X4 in computer wherein X1 means globulin-alpha 1 value; X2 means globulin-beta value; X3 means globulin-gamma value; X4 means total bilirubin value. Obtained values of diagnosis indices for the individual patient (Y1, Y2, Y3) are compared with average values of diagnosis indices (Y1', Y2', Y3') for different urogenital infections followed by comparison by sign and value. By the maximal coincidence of diagnosis index values for the individual patient with average diagnosis index values urogenital disease is diagnosed and the following diagnosis index average values are used: for chlamydiosis: Y1' = -2; Y2' = -0.1; Y3' = -0.2; for mycoplasmosis: Y1' = 2; Y2' = 0.8; Y3' = -0.04; for ureaplasmosis: Y1' = 2; Y2' = -1; Y3' = 0.02; for health persons: Y1' = -2; Y2' = 0.1; Y3' = 0.2. Invention provides the development of a method for express-diagnosis of infection at initial stage and diagnosis of atypical forms that occur in these diseases, and differential diagnosis of chlamydiosis, mycoplasmosis and ureaplasmosis. Invention can be used for carrying out the differential diagnosis of chlamydiosis, mycoplasmosis and ureaplasmosis.
EFFECT: improved method for express-diagnosis.
2 tbl, 4 ex
SUBSTANCE: method involves determining absolute value of ratio between lymphocyte number and absolute value of monocyte number in peripheral blood at the end of combine radiation therapy. The ratio is divided by 4.05. The result value being greater than 1, no disease relapse occurrence is predicted during the first observation year. The value being less than 1, tumor growth progress is stated and carcinoma relapse is predicted at the first year after treatment.
EFFECT: enhanced accuracy in detecting pathological process progress before observing clinical manifestations.
SUBSTANCE: method involves determining infrared radiation absorption coefficient in blood plasma in bandwidth of 1543-1396 cm-1. The infrared radiation absorption coefficient is determined in %. The value being equal to 29.7±1.1%, catarrhal cholecystitis is diagnosed. The value being 26.4±1.4%, phlegmonous cholecystitis is diagnosed. The value being 21.2±1.8%, gangrenous cholecystitis is diagnosed. The value being equal to 18.6±0.5%, gangrenous perforated cholecystitis case is diagnosed. The value in norm is equal to 32.4±0.8%.
EFFECT: high accuracy and specificity of diagnosis.
SUBSTANCE: the present innovation deals with biomedical measuring technologies, in particular, to those to detect bactericide activity of blood serum according to the level of its inhibiting impact upon luminescence intensity of sulfur-sensitive luminescent bacteria (ΣimpO) against control - luminescence intensity the same sulfur-sensitive luminescent bacteria that had no contact with blood serum (ΣimpK), then one should calculate the value of bactericide activity of blood serum by the following formula:
As sulfur-sensitive luminescent bacteria one should apply either natural or recombinant microorganisms being characterized by direct proportionality between intensity of decreased spontaneous bioluminescence level and degree of bactericide effect. For example, it is possible to apply Escherichia coli strain with genes of Photobacterium leiognathi luminescent system. The suggested method enables to shorten the duration for detecting bactericide activity of blood serum and decrease its labor intensity.
EFFECT: higher efficiency of detection.
1 cl, 1 ex, 1 tbl
SUBSTANCE: method involves determining blood insulin I and thyroxin T content and phagocytic leukocyte activity (PLA). Activity coefficient is calculated on the basis of formula KA=IxPLA/T. KA value being found greater than 2.8 units, considerable amelioration treatment effect is predicted. The value being from 1.4 to 2.8 units, amelioration is predicted. KA being less than 1.4 units, lower treatment efficiency is predicted.
EFFECT: high reliability of prognosis.