Method of determining bacterial number of dust in buildings
SUBSTANCE: method involves collecting dust samples with a sterile cotton wool wad from 10-20 cm2 of any surface or the sterile cotton wool filter of a vacuum cleaner. The dust samples are put into a sterile physiological solution, followed by extraction for 30-60 minutes at room temperature while stirring. The supernatant liquid is collected and multiple cultures of the obtained extract are prepared from the liquid. The obtained cultures are sown in a semi-liquid thioglycol medium and modified thioglycol medium obtained by adding defibrinated blood to the thioglycol medium in a given amount. Culturing is performed for 10 days at temperature 37°C and daily visual monitoring, followed by determining the number of microbial cells which are calculated from the McCready probability table, which is compiled based on data on growth properties of microorganisms and tinctorial properties of the bacterial community of the dust sample are determined through microscopic examination of smears of the mixed culture mass which is gram-stained.
EFFECT: simultaneous quantitative determination of the total bacteria number and obtaining cultural, morphological, tinctorial and haemolytic characteristics of the bacterial community of dust samples from buildings.
The invention relates to the field of ecology, namely, to obtain the initial characteristics of the microbial community dust areas, and can be used for monitoring residential and industrial premises with the aim of simultaneous quantitative determination of total bacterial contamination and receiving cultural, morphological, tinctorial and hemolytic characteristics of the microbial community samples of dust areas.
A known method for determining bacterial contamination Cup method through the consistent use of a set of special environments and cultivation under anaerobic and aerobic conditions (instructions for bacteriological control of complex sanitary-hygienic measures in health care institutions. Order No. 720 USSR Ministry of health, dated July 31, 1978, Appendix 2. HOWTO MUK 4.2.734-99. Microbiological monitoring of the working environment. The MOH of Russia. Moscow, 1999 I. Goh, Obbard J.P., Viswanathan, S., Huang Y. Airborne bacteria and fungal spores in the indoor environment. A case study in Singapore. Acta biotechnol., 2000, 20, 1, 67-73).
However, for screening studies of microbial contamination dust areas consistent use of several special nutrient media in different culture conditions greatly increases the effort and time, there is a need for the mine to obtain qualitative characteristics of bacterial flora.
The problem to which this invention is directed, is to develop a method which involves the use of monitoring environmental monitoring determination of bacterial contamination dust areas sowing dust sample on only one nutrient medium and simultaneously obtaining qualitative and quantitative characteristics of the microbial community.
The technical result of the claimed method is the achievement of efficiency, simplicity and convenience in determining the total bacterial contamination of the dust of the premises.
To achieve the technical result of the method of determination of bacterial contamination of the dust of the premises involves collecting samples of dust sterile cotton swab with 10-20 cm2any surface or on a sterile cotton filter when vacuuming, hanging dust or cotton swab in 1 ml of sterile physiological solution, extraction for 30-60 minutes at room temperature with stirring, the selection of the supernatant liquid, the preparation of supernatant multiple breeding extract, seeding the resulting dilution in thioglycolate environment and modified thioglycolate medium prepared by adding to 10 ml of thioglycolic environment defibrinating blood in quantities of 0.2-0.4 ml, stump the licensing within 10 days at a temperature of 37°C, daily visual inspection and then determining the most probable number number of microbial cells.
The determination of the most probable number number of microbial cells is calculated by the statistical table Mac-credit, based on the data on the growth properties of microorganisms, and tinctorial properties of bacterial community sample of dust detected by smear microscopy mixed culture of mass, gram-stained.
Preparation of thioglycolic environment. A portion of the environment to control the sterility of thioglycolic environment (thioglycolate Wednesday FS 42-326-SU-92: hydrolyzed casein enzymatic - 15 g, fodder yeast extract 5 g, sodium chloride and 2.5 g, glucose 5 g, thioglycolate sodium - 0.5 g cysteine hydrochloride, 0.75 g, agar-agar microbiological - 0.75 g, sodium carbonate and 0.8 g distilled water to 1 l, pH 7.0) was dissolved by heating in distilled water, poured into 10 ml sterile test tubes and sterilized. To prepare the modified environment to detect the presence of hemolytic microorganisms to 10 ml sterile thioglycolate environment at a temperature of 37-40°C, add 0.2-0.4 ml defibrinating blood (animal or human), stirred in a circular motion to obtain a uniformly colored solution.
The samples were collected dust can be the t to be made sterile cotton swab with 10-20 cm 2any surface or collected on sterile cotton filter when processing the surface of the vacuum cleaner.
Preparation of dilution of the extracts of samples of dust. A portion of dust or cotton swab placed in a sterile tube, add a known volume of sterile saline solution, the extraction is carried out for 30-60 minutes at room temperature with stirring, select the supernatant, from which it is prepared dilution of the extract.
Seeding each dilution is carried out in three parallel tubes prepared by the environment, and for planting the minimum breeding use tubes with a modified environment.
Visual inspection allows to make a conclusion about the presence in the microbial community of bacteria with different type of breathing, character growth, ability to flatulence, hemolytic properties.
The most probable number is the number of microbial cells (NWCC) is determined by the table Mac-credit.
Preparation of smears. Smears prepared from thoroughly mixed culture of mass of the tubes with a minimum dilutions, stained by Gram. When using the microscope determine tinctorial properties of the mixed culture is the presence of gram-positive and gram-negative coccoid and rod-shaped bacteria.
The invention is illustrated in SL is blowing examples.
Example 1. Determination of bacterial contamination of dust accommodations
A sterile cotton swab was collected dust with 10 cm2the surface of the plinth. The swab was placed in a sterile tube, add 1 ml of sterile physiological solution. After 30 minutes of stirring has prepared a five-fold dilution of the extract, spent sowing 100 ál of each dilution in three parallel tubes topicalias medium and 100 ál of minimum cultivation in three parallel tubes with the modified thioglycolate medium prepared by adding to 10 ml of thioglycolic environment 0.2 ml defibrinating blood. Were cultured for 10 days under aerobic conditions at a temperature equal to 37°C. Visual inspection revealed the presence of microbial community sample of dust, mainly aerobic microorganisms, as in the upper part of the column environment noted a strong turbidity on the surface of the film growth, at a height of 1 cm from the surface of the medium was observed 2-11 loose bars, the lower part of the environment was clear. Gasification has not been identified as of hemolysis. Microscopic analysis of the mixed culture showed the presence of coccoid and rod-shaped polymorphic bacteria gram-negative and gram-positive. The overall rate was 150 NVCC/cm2.
Example 2. Determination of bacterial contamination of dust collected mattress
A sample of dust collected on sterile cotton filter when processing the surface of the vacuum cleaner. A portion of the dust with a mass equal to 5 mg, was placed in a sterile tube, add 1 ml of sterile physiological solution. After 60 minutes of mixing has prepared a five-fold dilution of the extract, spent sowing 100 ál of each dilution in three parallel tubes with thioglycolate medium and 100 ál of minimum cultivation in three parallel tubes with the modified thioglycolate medium prepared by adding to 10 ml of thioglycolic environment 0.4 ml defibrinating blood. Were cultured for 10 days under aerobic conditions at a temperature equal to 37°C. Visual inspection revealed the presence in the microbial community of the sample dust bacteria with different types of respiration - aerobic and anaerobic (surface and bottom character growth), since the surface environment was noted film growth at the bottom of the tube was observed hemolysis. Microscopic analysis of the mixed culture showed the presence of coccoid and rod-shaped polymorphic bacteria gram-negative and gram-positive. The overall rate was 2×105NVCC/g of dust.
Thus, the claimed method simultaneously determined the number of total bacterial contamination, cultural, morphological, tinctorial and hemolytic characteristics of the microbial community samples of dust areas is a simple, effective and convenient for routine studies.
1. The method for determining bacterial contamination dust areas, including dust samples were collected with a sterile cotton swab with 10-20 cm2any surface or on a sterile cotton filter when vacuuming, hanging dust or cotton swab in 1 ml of sterile physiological solution, extraction for 30-60 min at room temperature with stirring, the selection of the supernatant liquid, the preparation of supernatant multiple dilution of the extract, the planting of the resulting dilutions in thioglycolate environment and modified thioglycolate medium prepared by adding to 10 ml of thioglycolic environment defibrinating blood in quantities of 0.2-0.4 ml, grown for 10 days at 37°C, daily visual inspection and then determining the most probable number number of microbial cells.
2. The method according to claim 1, characterized in that the determination of the most probable number number of microbial cells is calculated by the statistical table Mac-credit, based on the data on the growth properties of microorganisms, and tinctorial the basic properties of the bacterial community of the dust sample is detected by smear microscopy mixed culture of the masses, gram-stained.
SUBSTANCE: invention relates to field of medicine, namely to narcology. In order to predict recurrences of opium addiction in patient monthly carried out is incubation of lymphocytes in vitro in two samples: in presence of morphine in dose 10-6 M and in its absence - with further hystochemical staining for 5'-nucleotidase and semi-quantitative evaluation of said staining efficiency in points. Ratio of average points of staining for 5'-nucleotidase in lymphocytes, incubated with morphine and in its absence is calculated by division of first index by second. If said ratio decreases by more than 30% in comparison with the norm, appropriate preventive treatment is administered to patient.
EFFECT: method makes it possible to predict recurrences of opium addiction after withdrawal of patients from abstinence syndrome, due to detection of biological marker, anticipating clinical manifestations of recurrence.
SUBSTANCE: invention relates to field of medicine, namely, to laboratory methods of examination. In order to diagnose toxic affection of liver blood serum is analysed. 0.01 ml of blood serum in form of a drop is applied on microscope slide surface and dried under working room conditions. Obtained sample is microscoped and by presence of triad of morphological characters, which includes facies hyperpigmentation, multi-rayed cracks and dark-brown deposit, in form of polymorphic structures, located along multirayed cracks, conclusion about toxic affection of liver is made.
EFFECT: method makes it possible in short terms to diagnose toxic affection of liver at the early stage of disease.
2 dwg, 4 ex
SUBSTANCE: metal foil of certain thickness, length and width is used to produce certain defects with certain width opening, depth and length on reference specimens for testing. Prepared foil strips are fitted in pre-prepared grooves of rectangular or round shape to be filled with epoxy glue so that foil is immersed in glue to depth of 1-2 mm. With epoxy glue set, prepared specimen is ground to preset surface roughness and that of foil strip width. Then, foil is etched to produce defect.
EFFECT: certain defects with certain width opening, depth and length on reference specimens for testing.
SUBSTANCE: method of sampling air for quantitative determination of fluorine involves taking samples on a solid and liquid absorbent, wherein the solid sorbent used is granular sodium fluoride and the liquid absorbent used is a composite chemical: glycerin, buffer solution with pH 4.5, 0.643% solution of alizarin complexone, 0.72% solution of lanthanum nitrate, distilled water.
EFFECT: invention enables to separate hydrogen fluoride and hydrofluoric acid from fluorine during sampling and increases accuracy of determining fluorine.
SUBSTANCE: invention relates to field of medicine, namely to oncology. In order to predict risk of lymphatic cancer spread in case of stomach cancer, histological test of resected stomach is carried out. Sections of submucous base of mucous stomach lining, located at the distance 3-5 cm from visual edge of tumour, are analysed. If in histological preparations expanded capillaries formed by one layer of endothelial cells are present, metastases of stomach cancer in regional lymphatic nodes are predicted.
EFFECT: method increases accuracy of prediction of risk of lymphatic cancer spread in case of stomach cancer.
7 dwg, 3 ex
SUBSTANCE: there are made samples by melting experimental ingot of charge containing addition alloy for titanium alloy and additional component in form of spongy titanium which forms homogenous alloy with addition alloy. Also, amount of controlled addition alloy in alloy comes to from 10 to 50 wt %. There is fabricated a sample out of an experimental ingot and there is performed X-ray control for presence of inclusions in composition of addition alloy containing refractory metals. By results of X-ray control there is carried out micro-X-ray-spectral assay of composition of revealed inclusions.
EFFECT: more upgraded control of addition alloy for titanium alloys.
FIELD: machine building.
SUBSTANCE: procedure consists in sampling portion of preliminary compressed and formed analysed flow and in establishment of parity of linear flow rates of main and sampled flows by equalising pressure of main and sampled flows, thus, facilitating isokinetic selection. Also, whole cross section of the analysed flow is divided into virtual zones approximately equal in area. Further, sampling is successively carried out in the middle of areas of virtual zones. Notably, relative time of sampling in each zone is proportional to relative areas of virtual zones. The device consists of a stepped flange with through orifice in the centre. An upper bush of axial transfer is installed in the flange; with its lower end the bush is rigidly connected to a branch with through orifices. A lower bush is arranged on the lower end of the branch; also, the upper and lower bushes have orifices of the same diameter made asymmetrically relative to axis of the device. A capillary with a replaceable diffuser on its lower end is positioned in these orifices. A piston corresponding to a cylinder bush is arranged on a lower end of the lower bush. A stop interacts with internal surface of the piston; a packing ring is installed between the stop and the piston. On the lower end of the piston there is installed a replaceable diaphragm, while a cylinder jacket is installed between the stepped flange and the stop; the jacket has through orifices. An indicator is set on the upper end of the capillary, while a scale is set on the upper end of the stepped flange.
EFFECT: upgraded quality and accuracy of evaluation of flow rate and ratio of phases of measured flows.
2 cl, 1 dwg
FIELD: machine building.
SUBSTANCE: excitation and record are performed in one common point. Excitation is carried out with an impact of limited force sufficient for initial excitation of collective synchronous oscillation of atoms in lattice sites of minerals in composition of soils (rock) at frequency at least 25 000 Hz. Starting from the moment of excitation there are continuously and in time measured and recorded frequency of initial excitation of atom oscillation, system-changing to value of 0.1 Hz, and changing energy of descending frequency of initial excitation of atoms. On results of measured data processing there are allocated values of frequencies with amplitudes reflecting presence of structural (real) or other boundaries in massif of rock, interesting for analysis, time of changes of initial frequency of excitation to values of allocated frequencies, time of change (descending) of any allocated frequency to frequency determined with detail of analysis, and time of attenuation of fluctuations of rock massif within the range of frequencies or on separate frequency interesting for analysis. Using obtained quantitative and qualitative data, and generally accepted or specific, established by calculation and (or) experimentally analytic dependencies there is determined depth of selection of structural and (or) other boundaries of geological and (or) other objects. Vertical time-distance curve is plotted in each (or chosen) point of profile to required depth. There is determined velocity of fluctuation propagation, and physic, physic-mechanical, and geo-mechanical properties of massif of soil (rock).
EFFECT: increased resolving capacity and reliability of selection of structural elements in massif of rock, upgraded accuracy in determination of velocity characteristics of analysed massif of rock with complicated structure; raised validity and information value of observation results.
SUBSTANCE: testing vessel has a housing having an opening on its top end, as well as a storage container linked to the opening and which stores a sample of fluid medium. The vessel also has a cover which tightly closes the opening in the housing of the vessel with possibility of attachment/removal and an area which fixes the indicator strip. Said area is provided in the cover of the housing and fixes the other end of the indicator strip with possibility of attachment/removal, and also holds the indicator strip in the storage container while the opening in the housing of the vessel is tightly closed by the cover of the housing. The cover of the housing is formed from a cap fitted to the opening in the housing of the vessel with possibility of attachment/removal, and inner lining which is made from elastic material and tightly closes the gap between the cap and the opening. The area which fixes the indicator strip is provided in the inner lining. The indicator strip includes a multilayer body in which two or more elements are multilayered, and a covering layer which continuously covers the surface of one outermost layer of the multilayered body, the end surface of the multilayer body in the longitudinal direction, and the other outermost surface of the layer of the multilayer body. The method involves a step for storing the sample of fluid medium inside the storage volume of the testing vessel in a predetermined amount and a step for fixing the other end of the indicator strip to the area which fixes the indicator strip of the cover of the housing. The method also involves a step for tightly closing the opening in the testing vessel with the cover of the housing when the indicator strip is fixed and thus dipping the other end of the indicator strip in the sample of the fluid medium.
EFFECT: reduced effect of the surrounding medium on the sample, more correct tests.
7 cl, 11 dwg
FIELD: veterinary science.
SUBSTANCE: method is proposed to prepare samples for extraction-photometric method of detecting residual quantities of cationic surfactants in muscular tissue of animals and birds. Suspension of muscular tissue is prepared, and Carrez I solution is added to it. The mixture is thoroughly mixed. Then Carrez II solution is added and thoroughly mixed again. The produced suspension is soaked at room temperature for 30 min. The supernatant fluid is filtered via a paper filter. The produced filtrate is treated with two portions of diethyl ether. The filtrate is repeatedly filtered via filter paper. The 5% solution of sodium hydroxide is added until pH is 6.8.
EFFECT: invention increases sample sensitivity and assists in a higher extent of analyte from muscular tissue of animals and birds in extraction-photometric analysis.
3 tbl, 3 ex
SUBSTANCE: biosensor contains photo-autotrophic microalgae cells, the fluorescent characteristics of the photosynthesis system of which vary in the presence of cytotoxic chemical compounds in their surroundings: heavy metal ions and herbicides. Cells of green and diatomic microalgae are immobilised in cryogenic gel of polyvinyl alcohol: a cell suspension is deposited on a surface and the cells enter macropores of the polymer carrier under the effect of centrifugal force (5000-14000 g) for 1-10 minutes. A highly sensitive and stable biosensor is obtained based on components taken in the following ratio in wt %: microalgae cells 0.015-1.1; polyvinyl alcohol 7-15; aqueous phase - up to 100. Low concentrations of heavy metals and herbicides in aqueous systems are determined at flow rate of up to 360 ml/h based on the change in the value of relative variable fluorescence chlorophyll cells in the biosensor. The biosensor can be used for a maximum of 60 days.
EFFECT: high sensitivity of the biosensor.
2 dwg, 5 ex
SUBSTANCE: detection and identification of biological objects and their nanocomponents are enabled by exposure to radiation, monochromatic or nonmonochromatic radiation, including laser, sensing of one or more samples containing microobjects and their nanokcomponents with the use of a set of sample response measurement and record devices. The responses characteristics of each radiation conversion event are measured separately or in the aggregate, transferred and reduce in a diagnostically linear form. It is followed normalisation, correction and creation of a base of the reference and diagnosed parametres of microobjects and/or their nanocomponents. Further, the reference, diagnosed and identified microobjects and/or their nanocomponents are recognised and compared with experience data of required parametres on the basis of measurement with the use of a matrix.
EFFECT: use of the declared method allows precise qualitative and quantitative analysis of detected, identified, diagnosed parametres of the microobjects and their nanocomponents on the basis of optical measurement.
4 cl, 12 dwg, 1 tbl, 3 ex
SUBSTANCE: cultivated microbiological objects count is ensured by the measurements of their morphological compositions by determining the size distribution of microorganism cells in a nutrient fluid by variation of scattered light intensity. The fluid flow is sounded with monochromatic coherent light; interaction signal of probe radiation and analysed microbiological objects are recorded by the measurement of amplitude and duration of scattered light pulses by analysed particles; and functions derived from the measurements are plotted in the form of two-dimensional distribution of specified amplitudes and durations expressing statistic parameters of light scattering intensity by particles. After said functions, the size distribution of analysed cultivated microbiological objects and decay products of the nutrient fluid is derived.
EFFECT: higher measurement accuracy due to eliminated error caused by foreign particles that are decay products of the nutrient fluid in cultivation of the analysed microbiological objects.
9 dwg, 5 ex
SUBSTANCE: invention concerns medicine, particularly method of cardiomyocyte selection from cardiomyocyte-containing cell mix without genetic alteration incardiomyocytes. Method of cardiomyocyte content boost in cardiomyocyte-containing cell mix without genetic alteration. Method of cardiomyocyte obtainment without genetic alteration to cardiomyocytes. Method of cardiomyocyte content assessment in cardiomyocyte-containing cell mix.
EFFECT: possible efficient selection of cardiomyocytes from cardiomyocyte-containing cell mix without genetic alterations.
20 cl, 16 dwg, 14 ex
FIELD: chemistry, biochemistry.
SUBSTANCE: method can be used in microbiology, food industry for estimation of viability of unicellular organisms (yeasts, etc.), which demonstrate difference of dielectric properties. Per cent content in mixture of live and dead unicellular microorganisms is determined by deviation of measured value of dielectric permeability from dielectric permeability of mixtures, consisting only of live and only of dead unicellular microorganisms, or by experimental dependence of second derivative of dielectric permeability on humidity.
EFFECT: elaboration of distant methods of assessment in continuous flow, in elaboration of industrial methods of control over live microorganism production.
SUBSTANCE: invention concerns medical microbiology. The method of chronic urogenital gonococcal infection course forecast involves seeding of accompanying fungi of Candida genus in case of gonococcus detection, and persistence factors of accompanying microorganisms is evaluated. If titration of fungi of Candida genus gives not less than 102 colony-forming cells per millilitre and antilysozyme activity evolves simultaneously in the quantity not less than 1.3 mcg/ml per optical density unit, and anticomplementary activity not less than 1.5·106 antilytic complement units, then chronic character of urogenital infection is confirmed diagnosed.
EFFECT: increased accuracy of chronic urogenital gonococcal infection course forecast.
2 ex, 3 tbl
SUBSTANCE: method involves carrying out bacteriological study of esophageal mucous membrane biopsy samples. No microorganism growth or predominant Streptococcus spp., Peptostreptococcus spp., Staphylococcus spp. in monoculture or culture association in the amount of equal to or greater than 103-104 CFU/g (colony formation units), and no Escherichia coli, Bacteroides spp., Enterococcus faecalis, Enterococcus faecium, Candida spp. in monoculture or culture association in the amount of equal to or greater than 102-107 CFU/g, and optionally increased total microorganisms quantity to 104-107 CFU/g being observed, alkaline ingredient availability in refluxate in gastroesophageal reflux cases is declared to take place.
EFFECT: enabled alkaline ingredient availability and microbiocenosis disorders intensity evaluation.
SUBSTANCE: method involves determining duodenal juice acidity, duodenum bulbary and postbulbary department insemination degree with H.pylori. Ulcer edge and pyloric canal area bioptates are subjected to immunological and histological examination. Duodenal juice acidity being equal to or higher than 6.5 mmole/h and duodenal mucous membrane insemination degree being equal to or higher than 100 bacteria in vision field, IgG antibodies to H.pylori diluted in 1:160 proportion and higher, gastric metaplasia being detected in pyloric canal bioptates of ulcer edges among hypertrophied smooth muscle cells of separate groups of atrophied and deformed smooth muscle cells divided by layers of loose connecting tissue having blood vessels, fibroblasts, lymphocytes and macrophages, and anisochromia being detected when staining hypertrophied smooth muscle cells, pylorostenosis development is to be predicted.
EFFECT: high accuracy in predicting pylorostenosis development clinical course.
SUBSTANCE: method involves separating pure microorganism cultures from nasal mucous membrane and/or rhinopharynx microflora and identifying them. Anti-lysozyme activity is determined in pure culture and microbial insemination share in the general microbial insemination index is calculated for biotope under study. The first value being equal to or greater than 3 mcg/ml and the second one greater than 45%, rhinotubal microorganism migration into middle ear tympanic cavity is to be predicted.
EFFECT: high accuracy in predicting clinical course of inflammation in middle ear.
FIELD: biotechnology, microbiology.
SUBSTANCE: method involves sowing out samples of mixed cultures in liquid selective media, determination of cells number accumulating in media during microorganisms growth by bioluminescent method and mathematical treatment of kinetic data of the growth of individual bacterial cultures, determination of the parent concentration of microorganisms relating to different taxonomic groups. Invention allows carrying out the simultaneous identification of bacterial cells relating to different taxonomic groups and presenting in mixed cultures simultaneously, and to enhance precision in determination of cells number in the broad concentration range and to reduce the total analysis time significantly. In industry mixed cultures are used widely in different branches of food industry (dairy, meat, brewing and others) and in other biotechnological processes (biological treatment of sewage waters, bioremediation of soils, producing methane from waste in different manufactures and others). Invention can be used for differentiated determination of microorganisms number in mixed cultures that are widely distributed in nature: in air, soil, ponds, as components of natural microflora in higher organisms and among contaminants causing injury of different objects.
EFFECT: improved method for determination.
1 tbl, 3 dwg, 5 ex
FIELD: oil and gas production.
SUBSTANCE: samples from oil contaminated surface are chosen for selection of strains of micro-organisms-destructors of oil and oil products. There are selected pure cultures of hydrocarbon containing bacteria and they are cultivated on dense growth medium. There is determined catalase activity of grown strains of micro-organisms. Further, they are used for preparation of one billion microbe suspension which is mixed with Raymond liquid medium at ratio 1:150. As a sole source of carbon there is added oil or oil products from a place of contamination at 1 cm3 per 1 dm3 of Raymond medium and there is carried out incubation during 12 days. Further, culture is sown on dense growth medium and cultivated. Upon completion of cultivation there is determined catalase activity of studied strains. At its decrease in comparison to a source at 30 % and more analysed strain of micro-organism is chosen as active destructor of oil and oil product.
EFFECT: selection of strains of micro-organisms-destructors among aboriginal micro-flora most actively decomposing oil and oil products facilitating efficient measures for purification of water and soil ecotopes contaminated with oil and oil products.