Diagnostic technique for foetus growth inhibition
SUBSTANCE: amniotic fluid of a pregnant woman on her 16-19 weeks of pregnancy is analysed for the concentration of arginine and praline by capillary electrophoresis. An aspect ratio is calculated, and if its value is equal to 3.2 and higher, foetus growth inhibition is diagnosed.
EFFECT: higher diagnostic accuracy of foetus growth inhibition.
The invention relates to medicine, namely to obstetrics and Perinatology, and will find wide application in the development of midwifery practice.
The problem of delayed fetal development refers not only to the field of Perinatology, but also closely related to obstetric pathology and questions obstetric tactics (Ainstream et al. The fetal development. Kyiv. Health. 1988). The frequency of this complication of pregnancy according to some authors ranges from 4.5%to 39%, ranking in the structure of causes of perinatal morbidity and mortality in third place (Athboy, Mavodones // Obstetrics and gynecology. 1985. No. 12. S-30; Emilia, Tshapaieva // Obstetrics and gynecology. 1995. No. 6. P.18-24).
The effectiveness of remedial measures when diagnosed, usually at the end of II-III trimesters of pregnancy the fetal development is extremely reduced and only aimed at the prolongation of pregnancy, not its overcoming. In connection with this interest is the elucidation of early diagnostic markers of delayed fetal growth (rgts) in order to conduct timely remedial measures.
Analysis of patent and scientific and medical literature made it possible to analyze the existing methods of diagnosis of growth retardation of the fetus. A more modern technique of diagnosis rgts is the use of multiple fotometricheskikh until the of Atala, but they are informative only in the third trimester of pregnancy - 26-38 weeks (Ainstream, Annonin, Mavodones // Obstetrics and gynecology. 1988. No. 6. P.32-35). Ultrasound diagnosis rgts based on the comparison fotometricheskikh the results of the study, with the target indicators for the duration of pregnancy. One of the most informative parameters is biparentally the size of the head of the fetus. However, the measurement of this indicator cannot be used for the detection of growth retardation of the fetus.
Recently, to identify the syndrome rgts used diplomaticheskii determination of volumetric blood flow in the aorta of the fetus, the umbilical vein or the internal carotid artery with subsequent analysis of the curves of the flow velocity. Given that qualitative assessment of blood flow using systolicdiastolic ratio (C/D) and the resistance index reflects the same patterns in wide clinical practice for the diagnosis rgts also use the definition of pulsation index (PI), an increase which occurs when rgts in umbilical Vienna and a sharp decrease in the internal carotid artery. However, all these studies are the most reliable in the period from 17 to 20 weeks of pregnancy. Diagnostic value of disposable dynamic scintigraphy when is America fetal growth is 75-79% (Emilia, Tshapaieva // Obstetrics and gynecology. 1984. No. 6. P.18-24).
A known method for the diagnosis of threatening the status of the foetus in patent No. 2076624 (1997), which allows simultaneous registration of the electrical activity of the heart of the mother and fetus, and in the absence of a correlative relationship diagnose threatening the fetus or the degree of violation of the condition of the fetus. Regarding this analogue can be emphasized that the use of it in clinical practice allows to assess the presence of abnormalities in fetal development significantly in late pregnancy.
A known method for the diagnosis of intrauterine fetal development, described in the patent No. 2086981 (1997), which in the venous blood of the woman is determined by the absolute content of b-lymphocytes (VA) and the relative content of neutrophils have receptors for C3 component of complement (EAC-RON), and when the indicators VA greater than or equal to 0.3×109µg/l, and EAC-RON greater than or equal to 16%, the fetus is assessed as non-compliant physiological development and diagnosed the intrauterine development of the fetus. This method is not specific, since changes in the content of b-lymphocytes, and neutrophils may change at various diseases of the mother, not related to anti-aircraft regiment, as well as the accuracy of the method sostav the em only 80%.
In patent No. 2172958 (2001) proposed a method for the diagnosis of anti-aircraft regiment, which allows the diagnostics of early stages of growth retardation of the fetus by analyzing peripheral blood, including DNA isolation, conducting polymerase chain reaction and determination of allele PL-AII gene glycoprotein IIIA. It should be noted that this method has a major drawback, especially the limited application because of the complexity of its implementation, expressed in mnogoetapnoe and duration of its execution.
Another way to early prediction of delay syndrome fetal development is defined in the peripheral venous blood content HLA-DR + monocytes (patent 2265224, 2004), and its value is equal to or smaller 57,9%, predict the development of the syndrome of rgts. It should be noted that this method is also limited in application because of the complexity and the duration of its execution.
In the patent of Russian Federation №2331365 (2007) proposed a method for the diagnosis of syndrome of intrauterine development of the fetus by definition in the blood of a pregnant woman content of allantoin, and when the values of its concentration from 2.9 to 5.8 mmol/l diagnose the syndrome of intrauterine development of the fetus. This method is not specific, as expressed by the decrease of this indicator is observed not only in anti-aircraft regiment, and when karasuyama the pregnancy and early spontaneous abortion.
The prototype of the present invention is elected as a method for predicting the intrauterine development of the fetus, described in the patent No. 2246733 (2003). The essence of the prototype method is that in the serum of pregnant determine the content insulinopenia growth factor and vascular endothelial growth factor, calculate the ratio of their relationship and the value of its 28.5 and below diagnosed fetal development.
This method has a number of imperfections:
1. The method is not sufficiently specific - level change growth factors observed for the various complications of pregnancy - eclampsia, non-developing pregnancy, threatened miscarriage, premature birth (Avilov et al. // Ross. Bulletin akush. and gin. - 2005. No. 3. P.7-9).
2. The method is laborious and time-consuming it is running.
3. The implementation of the method requires expensive equipment and import sets of reagents for immunoassay analysis, which hinders its widespread use in clinical practice.
These drawbacks are eliminated in the invention.
The objective of the invention: improving the accuracy and specificity through the development of a specific method for the diagnosis of growth retardation of the fetus (rgts).
The problem is solved in that in the amniotic fluid of women in pregnancy (16 weeks the ü of gestation) by capillary electrophoresis determine the content of arginine and Proline, calculate the ratio of their relationship and the magnitude of the coefficient, equal to 3.2 and above, diagnosed with fetal growth retardation.
The technical result is obtained during use of the invention is to improve the accuracy of diagnosis of growth retardation of the fetus, which allows you to assign a timely and adequate therapy in the earlier period.
The novelty of the proposed method lies in the fact that for the first time proposed to diagnose rgts pregnant at an early age, exploring the amniotic fluid (once) and specifying in it the ratio of the amino acids arginine and Proline.
It is known that amino acids perform the vital role of "construction material" in the biosynthesis of a specific tissue proteins, enzymes, peptide hormones, etc. the Role of amino acids as essential substrates in metabolic processes is inextricably linked with another no less important is their function as regulators of many biochemical and physiological reactions (plastic, energy metabolism, and others). Among the nitrogenous components of the amniotic fluid free amino acids are important, so how to effectively use the fruit as a plastic material (Snaaake, .Asai // Acta Obstet. Ginec. Jap., 1979. - 31. No. 2. P.226).
Special attention is currently given to the role of arginine in beremend the STI, and also to diagnose the condition of the fetus (Iaustralia et al. // The Bulletin of PFUR. - 2005. No. 4. - Pp.92-98; Avishalom // Izvestiya vuzov North KAV. the region. - The natural. science. - 2006. No. 8. - S.112-116; Svilenova et al. // Ross. Bulletin akush. and gin. - 2007. No. 2. - P.4-7). Arginine is one of the most effective stimulant product somatotropic pituitary hormone (growth hormone) and allows it to maintain its concentration at the upper normal limits, and the lack of arginine leads to stunted growth of children (Mcherron et al. // Pharmacy. - 2004. - T. No. 5. - P.18-20).
An important role is also played by another amino acid - Proline. Particularly rich in them the main protein of connective tissue - collagen, which in high concentrations is contained in the bones and muscle tissue. Proline is a non-polar amino acid, is a building material cellular proteins. Recently published data on the practical use of Proline. It is widely used in medicine in the composition of amino acid mixtures for parenteral nutrition, in cryomedicine (Y. Morita et. al. 2005) and as the basis for the creation of antipsychotic drugs, as well as in the food industry as an antioxidant (Sagamino // ABT. Diss. Kida. Biol. Sciences. - 2005. - 240 S.).
This method is carried out by examination of samples of the amniotic fluid.
Under aseptic conditions, t is academically access produce the capture of amniotic fluid under ultrasound for the diagnosis of chromosomal pathology.
Before analysis the samples of amniotic fluid is treated with a 10% solution of trichloroacetic acid to deproteinization. Next, the samples centrifuged for 15 minutes at 5500 rpm C. the Obtained supernatant solution was used for analysis.
Identification and quantitative determination of free form arginine and Proline performed by capillary electrophoresis using unmodified silica capillary ("Capel-105", "Lumex", SPb., Russia).
Before you begin the capillary sequentially washed with 1 M hydrochloric acid, distilled water, 0.5 M sodium hydroxide solution and the buffer solution (20 mm borate buffer, pH 9,15). The time of sample introduction 15 C. a Pressure of 30 mbar, operating voltage 20 kV. Use direct spectrophotometric detection at 200 nm. All studies are carried out at a temperature of 30°C.
Data processing is done using an IBM PC with the software "Multichrome" (JSC "ampersand character"). Then calculate the ratio of arginine to Proline.
The efficiency of this method is confirmed by the following clinical examples.
Example 1. The patient Peninsula, 20 years. Outpatient map No. 193. Addressed in the consultative polyclinic NIIAP (22.12.2008, in connection with suspected chromosomal abnormality of the fetus, the time taken is nasty 16 weeks.
25.12.2008, in aseptic conditions transabdominal access made taking amniotic fluid under ultrasound for the diagnosis of chromosomal abnormalities and determination of arginine and Proline by capillary electrophoresis. By capillary electrophoresis identified in amniotic fluid content of arginine, which amounted to 216 mg/ml, and Proline - 70 mg/ml
The ratio of arginine to Proline = 3,1, indicating the absence of the occurrence of delayed fetal growth. During the whole period of gestation the patient was under observation in the clinic NIIAP.
11.05.2009, the woman came to give birth in the maternity unit of NIIAP.
D-C: pregnancy 39 weeks. Birth spontaneous, ended in a live birth, full-term baby with a mass 3200,0.
This clinical example confirms the accuracy of diagnosis of the absence of the occurrence of rgts.
Example 2. Patient G to 30 years. Outpatient map No. 18. Addressed in the consultative polyclinic NIIAP (19.01.2009, in connection with suspected chromosomal abnormality of the fetus, gestational age 22 weeks.
21.01.2009, in aseptic conditions transabdominal access made taking amniotic fluid under ultrasound for the diagnosis of chromosomal abnormalities and determination of arginine and n is oline by capillary electrophoresis. By capillary electrophoresis identified in amniotic fluid content of arginine, which amounted to 61 µg/ml, and Proline - 19 mcg/ml the Ratio of arginine to Proline = 3,2.
The diagnosis of the possible occurrence of rgts.
29.04.2009, the woman came to give birth in the maternity unit of NIIAP.
D-C: pregnancy 37-38 weeks. Birth spontaneous, ended in a live birth with a mass 2200,0.
This clinical example confirms the ability to accurately diagnose rgts on the proposed factor.
By the present method were examined 38 women, among whom 29 ratio of arginine/Proline in the amniotic fluid is equal to 3.1 and below, and in 9 patients it was 3.2 and above. All pregnant women with factor of 3.2 and above identified fetal growth retardation, confirmed the birth of underweight children (2370-2530,0). Women with a ratio of arginine/Proline was equal to 3.1 and below, gave birth to children without signs of rgts.
The above confirms the diagnostic value of the claimed method.
Thus, the inventive method is more effective in comparison with the known and has a number of advantages:
1. It has high accuracy. The accuracy is 99%.
2. Method-specific (definition rgts this factor is a specific method).
3. the manual high-sensitivity, allows definition rgts at the preclinical stage.
Approbation of the invention carried out on a sufficient volume of clinical material, which confirmed the efficiency of the method and the possibility of its application in clinical practice.
A method for the diagnosis of growth retardation of the fetus by a study of the biological fluid of a pregnant woman, characterized in that a pregnant woman in terms 16-19 weeks in amniotic fluid by capillary electrophoresis determine the content of arginine and Proline, calculate the ratio of their relationship and the magnitude of the coefficient, equal to 3.2 and above, diagnosed with fetal growth retardation.
SUBSTANCE: substance of the method for prediction of a CNS pathology in newborns consists in the fact that that blood of a pregnant woman on her 3rd trimester is examined for a nitrogen oxide. If the blood NO concentration is 6.12 mcmol/l and more, the developing CNS pathology is predicted in a newborn.
EFFECT: use of the declared method enables effective screening assays of the pregnant women for identification of the CNS pathology in newborns.
SUBSTANCE: peripheral blood lactate level is measured. If the postischemic lactate level exceeds 10 mmol/l, reperfusion syndrome is diagnosed.
EFFECT: improved diagnostic accuracy.
5 dwg, 1 ex
SUBSTANCE: substance of the laboratory diagnostic technique for sepsis consists in a quantitative analysis of blood serum for nonprotein microorganism metabolism products, particularly n-hydroxyphenyllactic (HPLA), phenyllactic (PLA), n-hydroxyphenylacetic (HPAA) and homovanillic (HVA) acids. If the contents of said four acids listed above synchronously excess the a healthy level by 5 times or more, sepsis is diagnosed.
EFFECT: use of said technique allows higher reliability of diagnosing sepsis in the patients with hyperthermia of an uncertain aetiology, in impaired state of the patients in hopital, in the intensive care patients, and in surgical postoperative complications.
4 tbl, 3 ex
SUBSTANCE: invention describes a method of quantitative evaluation of blood acetic, propionic, isobutyric, butyric, valeric, isocapronic and capronic acids by gas chromatography analysis wherein a blood sample is acidified with 1 % sulphuric acid to pH 2-3, evaluated acids are extracted with isobutyl alcohol volume of which is related to the blood sample volume as 1:1. The protein separation is enabled by centrifugation. 2-3 drops of 0.4 % alkali is added, and the extract is evaporated dry, further the solid residue is added consistently with 1 % sulphuric acid and isobutyl alcohol that is followed with gas chromatography separation of the mixed acids in a capillary column with a flame ionisation detector, and the amount of each acid is evaluated by a calibration diagram.
EFFECT: higher sensitivity and accuracy of the method of quantitative evaluation of acetic, propionic, isobutyric, butyric, valeric, isocapronic and capronic acids if found in blood together.
5 cl, 1 ex, 4 tbl
SUBSTANCE: invention relates to field of medicine, namely, to gastroenterology. For non-invasive diagnostics of fibrosis and cirrhosis in case of HBV and HCV infections complex ultrasonic examination of liver and spleen tissue is carried out. Additionally duplex scanning with colour Doppler mapping of porto-hepatic region vessels is performed and quantitative indices of hemodynamics of rate of blood flow in vessels, including splenic vein, are determined. Blood test is analysed and used to determine number of platelets, biochemical blood tests are performed, most significant for determination of disease degree indices of coagulogram are taken. After that, discriminant analysis of obtained characteristics and indices is carried out, and taking into account age and experimentally obtained coefficients, total value of two canonical discriminant functions F1 and F2 for HCV and HBV is calculated. Further, by obtained in empiric way territory map position of point for calculated by patient's concrete indices values F1 and F2 for cases of HCV-infection and HBV-infection is determined. Depending on point location on territory map case of mild fibrosis, severe fibrosis or liver cirrhosis is diagnosed.
EFFECT: method increases reliability of fibrosis and cirrhosis diagnostics in case of HBV and HCV infections.
10 dwg, 2 ex
SUBSTANCE: peripheral blood thrombocytes of women suffering gestosis of various severity levels on their 32-38 weeks of pregnancy are analysed for the activity of glutathione reductase (GY), NADF-dependent glutamate dehydrogenase (NADFGDG) and NADF-dependent isocitrate dehydrogenase (NADFICDG). A nicotine amide adenine dinucleotide phosphate transfer coefficient (NTC) represented by the relation of the GY activity to a product of the NADFGDG and NADFICDG activities is calculated. At the NTC value is equal to 1.3 and lower, the newborn's Apgar score is predicted to be equal to 6 and less, and the NTC value exceeding 1.3 provides the Apgar score being 7-10 points.
EFFECT: more accurate prediction of the newborn's state.
2 tbl, 5 ex
SUBSTANCE: areas highly exposed to harmful chemical agents are chosen. A random group of children without clinical signs of living in this territory is tested using chemical laboratory tests of blood to identify the content of chemical compounds, which are priority chemical environmental factors on the selected area of residence and clinical and laboratory studies are conducted to determine a set of laboratory indicators of adaptation system. Then using the results of the study the average values of chemical compounds in the blood are fixed and then they are compared to the background and average values for each of the above laboratory parameters are fixed and compared to the physiological norm; deviation of this value from the normal rate reveals children bodies response to chemical exposure. Next, a causal relationship is established between the level of content of chemical compound in the blood and the response of the child body through deviation of laboratory parametres from the norm using a logistic regression model. Using method based on analysis of odds ratios the maximally inactive level of marker of exposure and corresponding response marker based on the conditions are determined under which the odds ratio that characterises the degree of the connection between exposure to a chemical compound and the body's response will be greater than or equal to one; for this a model of dependence between the level of a marker of exposure and the specified index odds ratio is designed, the parametres of the model are determined and they reflect the change in the probability using which the value of the maximally inactive level of marker of exposure is calculated, i.e. maximum ineffective concentrations of chemical compounds. From the entire spectrum of defined concentrations of certain chemical compounds for each laboratory parametre of adaptation systems choose the smallest value that is accepted as the maximally inactive concentrations on a child adaptation system for a given chemical compound i. In future diagnosis of violation of adaptation of children living in the selected area is performed by comparing the content Ci of certain chemicals in their blood with previously established value of inactive concentration for this chemical compound; and if a ratio is a violation of adaptation is diagnosed.
EFFECT: method allows diagnosing violation of children adaptation under chemical hazards of environmental factors with high precision at an early preclinical stage, with simultaneous simplicity and accessibility for a wide practical application.
5 tbl, 2 dwg
SUBSTANCE: zeolite antioxidant activity test is enabled by introducing a substance being tested into bodies of experimental animals. Biological products of tissues and organs of the experimental animals and control sets are prepared. Metabolic process indicator substances are evaluated. The pulmonary tissue, blood plasma, erythrocytes and thrombocytes are analysed for the content of lipid peroxidation products and natural antioxidants which are scored and summed up. The zeolite antioxidant activity is tested relatively to the normal values of the content of lipid peroxidation products and natural antioxidants which are defined as an arithmetical mean of the relevant values received in the animals of a control set.
EFFECT: enabled reliable zeolite antioxidant activity test at the enabled comparative evaluation of substances by this parametre.
2 tbl, 1 ex
SUBSTANCE: vaginal fluid is analysed. The vaginal secretion is collected by means of a common tampon placed in a vagina for 8-9 hours, further weighted; microbial metabolites are extracted in equiponderate amount of distilled water, and the extract is analysed by gas-liquid chromatography. If the vaginal discharge contain acetic acid more than 0.315 mg/g and total propionic and butyric acids ≤0.200 mg/g in an age group of 17 to 34 years, and acetic acid more than 0.210 mg/g with total propionic and butyric acids ≤0.120 mg/g in an age group of 35 to 48 years, nonspecific aerobic vaginitis is diagnosed.
EFFECT: more accurate diagnosis of nonspecific aerobic vaginitis.
SUBSTANCE: method of evaluating immunogenicity of brucella strains includes enzyme-linked analysis of culture supernatant of peripheral blood cells for content of cytokines - tumour necrosis factor (TNF-α), interleukin-1β (IL-1β) and colony-stimulating factor (CSF), synthesized by mononuclear cells of peripheral blood in vitro without impact (spontaneous production) and under the impact of antigens of evaluated brucella strains (induced production) and determination of their immunogenicity by ratio of spontaneous and induced production of said cytokines, brucella strains are considered immunogenic if their antigens cause enhancing of TNF-α by 1-1.25, IL-1β by 2-2.50 and CSF by 3-3.70.
EFFECT: method improvement.
FIELD: medicine, hepatology.
SUBSTANCE: one should detect the level of hepato-specific enzymes (HSE) in blood plasma, such as: urokinase (UK), histidase (HIS), fructose-1-phosphataldolase (F-1-P), serine dehydratase (L-SD), threonine dehydratase (L-TD) and products of lipid peroxidation (LP), such as: dienic conjugates (DC), malonic dialdehyde (MDA). Moreover, one should detect the state of inspecific immunity parameters, such as: immunoregulatory index (IRI) as the ratio of T-helpers and T-suppressors, circulating immune complexes (CIC). Additionally, one should evaluate the state of regional circulation by applying rheohepatography (RHG), the system of microhemocirculation with the help of conjunctival biomicroscopy (CB) to detect intravascular index (II). In case of increased UK, HIS levels up to 0.5 mcM/ml/h, F-1-P, L-SD, L-Td, LP products, CIC by 1.5 times, higher IRI up to 2 at the norm being 1.0-1.5, altered values of regional circulation, increased II up to 2 points at the norm being 1 point, not more one should diagnose light degree of process flow. At increased level of UK, HIS up to 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 1.5-2 times, increased IRI up to 2.5, altered values of regional circulation, increased II up to 3-4 points one should diagnose average degree of process flow. At increased level of UK, HIS being above 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 2 and more times, increased IRI being above 2.5, altered values of regional circulation, increased II up to 5 points and more one should diagnose severe degree of process flow.
EFFECT: higher accuracy of diagnostics.
FIELD: medicine, infectology, hepatology.
SUBSTANCE: in hepatic bioptate one should detect products of lipid peroxidation (LP), such as: dienic conjugates (DC), activity of antioxidant enzymes, such as: catalase (CAT)and superoxide dismutase (SOD). One should calculate by the following formula: C = DC/(SOD x CAT)x100, where DC - the content of dienic conjugates, SOD - activity of superoxide dismutase, CAT - activity of catalase. At coefficient (C) values being above 65 one should predict high possibility for appearance of cirrhosis, at 46-645 - moderate possibility and at 14-45 -low possibility for appearance of cirrhosis.
EFFECT: higher accuracy of prediction.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
FIELD: medicine, juvenile clinical nephrology.
SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
EFFECT: higher efficiency of detection.
7 dwg, 1 ex, 6 tbl
FIELD: clinical medicine, pulmonology.
SUBSTANCE: one should carry out complex estimation of interleukin-1β) concentration in blood, saliva, bronchoalveolar liquid. Moreover, one should detect distribution coefficient (DC) for IL-1β as the ratio of IL-1β blood content to IL-1β salivary content. At increased IL-1β blood content by 10 times and more, by 2 times in saliva, unchanged level of bronchoalveolar IL-1β, at DC for IL-1β being above 1.0 one should predict bronchial obstruction. The method enables to conduct diagnostics of the above-mentioned disease at its earlier stages.
EFFECT: higher efficiency of prediction.
FIELD: medicine, diagnostics.
SUBSTANCE: the present innovation deals with genetic trials, with diagnostic field of oncological diseases due to analyzing DNA by altered status of gene methylation that take part in intracellular regulation of division, differentiating, apoptosis and detoxication processes. One should measure the status of methylation in three genes: p16, E-cadherine and GSTP1 in any human biological samples taken out of blood plasma, urine, lymph nodes, tumor tissue, inter-tissue liquid, ascitic liquid, blood cells and buccal epithelium and other; one should analyze DNA in which modified genes of tumor origin or their components are present that contain defective genes, moreover, analysis should be performed due to extracting and purifying DNA out of biological samples followed by bisulfite treatment of this DNA for modifying unprotected cytosine foundations at keeping 5-methyl cytosine being a protected cytosine foundation followed by PCR assay of bisulfite-treated and bisulfite-untreated genes under investigation and at detecting alterations obtained according to electrophoretic result of PCR amplificates, due to detecting the difference in the number and electrophoretic mobility of corresponding fractions at comparing with control methylated and unmethylated samples containing normal and hypermethylated forms of genes one should diagnose oncological diseases. The method provides higher reliability in detecting tumors, detection of remained tumor cells after operation.
EFFECT: higher efficiency of therapy.
1 cl, 3 dwg, 4 ex
FIELD: medicine, gastroenterology.
SUBSTANCE: one should carry out diagnostic studying, moreover, on the 5th -6th d against the onset of exacerbation in case of gastric and duodenal ulcerous disease one should detect the content serotonin, histamine and acetylcholine in blood, then during 2-3 wk one should conduct medicinal therapy to detect serotonin, histamine and acetylcholine level in blood again and at serotonin content being by 2-3 times above the norm, histamine - by 1.15-1.4 times above the norm and acetylcholine - by 20-45% being below the norm one should predict the flow of gastric and duodenal ulcerous disease as a non-scarring ulcer.
EFFECT: higher accuracy of prediction.
SUBSTANCE: method involves taking blood from ulnar vein (systemic blood circulation) and from large vein of the injured extremity proximal with respect to lesion focus (regional blood circulation). Spontaneous NST-test value is determined and difference is calculated in systemic and regional blood circulation as regional-to-systemic difference. The difference value is used for predicting clinical course of pyo-inflammatory disease in extremities.
EFFECT: high accuracy of diagnosis.
4 cl, 2 tbl
FIELD: medicine, gastroenterology.
SUBSTANCE: one should introduce biologically active substance, moreover, in patient's blood serum one should detect the content of acetyl choline and choline esterase activity followed by 2-h-long intragastric pH-metry at loading with biologically active substance as warm 40-45%-honey water solution at 35-40 C, and at increased content of acetyl choline being above 1.0 mM/l, choline esterase being above 0.5 mM/l/30 min and pH level being 6.0-6.9 it is possible to consider apitherapy to be useful for treating ulcerous duodenal disease.
EFFECT: higher efficiency and accuracy of detection.
FIELD: medicine, gastroenterology.
SUBSTANCE: it has been suggested a new method to detect pharmacological sensitivity to preparations as acidosuppressors. After the intake of the preparation a patient should undergo fibrogastroduodenoscopy 3 h later, then, through endoscopic catheter one should introduce 0.3%-Congo red solution intragastrically and the test is considered to be positive at keeping red color that indicates good sensitivity to the given preparation, and in case of dark-blue or black color the test is considered to be negative that indicates resistance to this preparation. The suggested innovation widens the number of diagnostic techniques of mentioned indication.
EFFECT: higher efficiency of diagnostics.