Method of increasing antioxidant status of warm-blooded organism under ultraviolet irradiation |
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IPC classes for russian patent Method of increasing antioxidant status of warm-blooded organism under ultraviolet irradiation (RU 2424580):
 Method for prevention of chronic toxic cardiopathy in experimental animals / 2424579
In studying methods for prevention the injuries associated with chronic toxic action of heavy metals in experimental animals, a chronic molybdenum intoxication is simulated by a daily introduction of an ammonium molybdate solution through a probe in a stomach in a dose of 50 mg/kg on a metal base in which a unit of the solution 1 ml is equal to molybdenum 50 mg. Simultaneously every second day for two months, the natural sorbent yrlite-1 in the form 6% suspension is introduced in an animal through a probe into a stomach in an amount 2.5% of body weight.
Method of simulating gastropathy / 2424578
Gastropathy is simulated in nonlinear white rats by a 21-day oral introduction of the mixture 200 mcl of 5% ascorbic acid and the nonsteroidal anti-inflammatory drug Nise 20 mg/kg, an hour before feeding once a day.
 Method of modelling infected ureterohydronephrosis / 2423739
Ureter is freely wrapped with two layers of a cellophane strip 1-1.5 cm wide. Thereafter, a microdose of a microbial suspension is introduced on an isolated segment of the ureter in its wall and paraurethral fatty tissue.
 Method of estimating anti-ergotypic response in experiment / 2423079
Invention relates to biology and medicine, namely to experimental models of immunologic states, and can be used for estimation of anti-ergotypic response to vaccination with polyclonally activated cells. For this purpose vaccination with splenocytes, activated by means of anti-CD3 antibodies and interleukin-2, by subcutaneous injections 1 time per week during 4 weeks. After that response of delayed-type hypersensitivity (DTH) is induced by introduction of activated splenocytes under aponeurosis of animal paw pad. Anti-ergotypic response is estimated by degree of DTH development.
Method for prevention of rat's liver, myocardium and pancreas injury in chronic alcoholic intoxication modelling / 2422916
For correction of metabolic and morphological disorders in chronic alcoholisation, hypovitaminosis diet is applied in rats. 15 % ethanol is used as a drink. Tykveol 1 ml per 1 kg of animal's body weight pre-mixed with food is introduced.
Method of experimental proliferative process inhibition in prostate tissue / 2422915
For experimental proliferative process inhibition in prostate tissues, the endoliquor introduction of the estrogenic preparation Estradurine to senior male rats in weight 200-250 g is performed. The preparation is introduced in a single dosage 0.002 mg by a suboccipital puncture. On the 10th day, the rats are decapitated for prostate examination.
 Method of experimental neolymphogenesis modelling / 2422914
An intermittent transplantable tumour is formed in an experimental animal. After the transplantable tumour reached a size not less than 0.5 cm in diameter, growing new lymph nodes are observed in a regional areas from the tumour.
 Method of acute intestinal obstruction modelling / 2422913
For acute intestinal obstruction modelling, an inferior midline laparotomy and intestinal ligation through a laparotomy incision are performed. A soft tube 6 cm long is introduced through anus. The intestines are ligatured with thick ligatures on the tube, and the ligature proximal to anus is ligatured on the tube, while the distal ligature is fixed on the intestine not covering the tube at the distance of 1 cm from the proximal ligature directly at a tube end distal to anus. Thereafter, a mesentery with the vessels feeding the given segment of the intestines is transected.
Method of suppressing cord-factor of tuberculosis mycobacteria pathogenicity / 2422150
Invention relates to field of veterinary and medicine, namely to phthisiology, and can be applied for suppression of formation of cord-factor of tuberculosis mycrobacteria pathogenicity in vitro. For this purpose as antagonist applied is suspension of Bacillus subtilis "ТНП-3-ДЕП" strains and Bacillus subtilis "ТНП-5-ДЕП" is in equal ratio with concentration of 5·108 CFU in 1 ml.
 Method of simulating tubercular osteitis of various severities / 2421823
Cortical layer is perforated near an articular capsule of a knee joint; a canal is formed in a spongy substance of a distal epiphysis of a femoral bone. With using a trocar, a fragment of a haemostatic sponge with a fluid culture of tuberculosis mycobacteria 1×103 to 1×108 cells/mcl is inserted in this canal. Then, an autogenous bone graft is used to fill a bone defect with isolating an infection area with a cement filling.
 S1p3 receptor antagonist / 2424227
Invention relates to novel compounds - arylamidrazone derivatives of formula
Method of treating surgical endotoxicosis / 2423140
Invention relates to medicine, namely to surgery, and can be applied for treatment of surgical endotoxicosis, complicated with intestinal insufficiency. For this purpose applied is solution of mixture of powder sublimates of juice, water extract of plant tops and water extract of marc of young table beet. Mixture of powder sublimates is dissolved in quantity 40 g of dry substance per 1 l of neutral anolyte ANK with concentration 0.01-0.02% to obtain standard solution 1-2% by pectin. Obtained solution is introduced enterally drop-by-drop through capillary probe with 5 days course. Solution introduction insures enterosorption, nutritive support, stimulation of intestine motor functions.
Medication possessing lympho kinetic activity / 2423138
Invention relates to pharmaceutical industry, in particular to medication, possessing lymphokinetic activity. Application of dihydroquercetin as medication, possessing lymphokinetic activity.
 Medication for irreversible inhibition in vivo of carboxylesterase based on phosphorolated 1-hydroperfluorisopropanols, which has low acute toxicity for warm-blooded organisms, and method of its obtaining for inhibition of carboxylestarase in plasma of rodents / 2423132
Invention relates to field of medicine. Phosphorilated 1-hydroperfluorisopropanols of formula 1 are applied as effective medication for irreversible inhibition in vivo of carboxylesterase. Said compounds represent (O,O-diethyl)-01-trifluormethyl-2,2,2-trifluorethylphosphate. In order to inhibit in vivo carboxylesterase in plasma of rodents, effective amount of said compounds is introduced to animals.
Medication for normalising exchange processes in animals / 2423109
Invention relates to field of veterinary. Medication contains diacetophenonyl selenide (DAPS-25), carotenoid and vegetable oil with the following component content, wt %: DAPS-25 0.18-0.24, pycopene 0.2-0.3, vegetable oil - the remaining part.
Method of preventing severe forms of obstetrical pathology / 2422149
Invention relates to medicine, namely to obstetrics, and can be applied for prevention of severe forms of obstetrical pathology. Method includes preliminary dopplerometry of uterine-placental hemodynamics condition for pregnant women at 2 trimester of pregnancy. After that, infusion of magnesia in dose 30.0 ml is performed. One day after infusion repeated dopplerometry of uterine arteries is carried out and if the spasm of uterine vessel remains - donor of nitrogen oxide - nebilet in minimal dose in dose 2.5 mgx2 times per day is prescribed under control of AP and pulse.
 Foam licorice bath / 2421204
Invention refers to medicine, namely - to balneotherapy. A method involves the use of a dense extract of licorice root (DELR). The DELR is used in the amount 50 to 150 ml with the content of glycyrrhizic acid 12 - 14 % and more. Foam is generated by air pressure 300-400 kPa before a patient immersed in the bath, with pressure drop to 100-150 kPa and conservation throughout the whole procedure after the patient immersed in the bath. If observing the secondary immunodeficiency in the patients with allergic diseases, procedure duration is up to 10 minutes. A pathology caused by the secondary immunodeficiency without allergic diseases requires a 10-15-minute procedure.
 Fused proteins binding immunoglobulin domain / 2420537
Offered is a fused protein containing from an amino-terminus to carboxyl terminus: binding domain polypeptide which is bound with a biological target molecule; human one-cysteine "link" region IgGl peptide; immunoglobulin heavy chain CH2 constant region polypeptide and immunoglobulin heavy chain CH3 constant region polypeptide. The fused protein represents mixed monomers and dimers, has ADCC, CDC or both cytotoxicities. Also, described are a pharmaceutical composition and a method of treating a B-cell disorder with using the fused protein.
 Pyrazolopyrimidine derivative / 2420530
Invention refers to a compound presented by formula (1), to its salt or hydrate where in formula
 Compound for treating lipase-mediated diseases / 2420513
Invention relates to novel compounds - benzoquinone derivatives of formula (I):
Method of treating rectal cancer / 2424013
Invention refers to medicine, oncology, and can be used for treating rectal cancer. That is ensured by an intramuscular introduction of ingaron 500000-1000000 IU ml for 5-10 days to a patient once a day. It is followed with an irradiation procedure in a dynamic dose fractionation mode. The first three coarse irradiation fractions are combined with carboplatin 300-450 mg/m2. Daily during the whole irradiation course, xeloda 500-1500 mg/m2 is introduced as a radio sensitiser.
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FIELD: medicine. SUBSTANCE: 20 minutes prior to an irradiation procedure in an ultraviolet chamber, an infusion tea of nettle, birch, plantain leaves is introduced orally in a laboratory animal. The infusion tea is prepared of leaves taken in the ratio 1:1:1, by keeping 8 g of the tea in boiling water 200 ml. The infusion tea is introduced at 5 ml/kg of weight daily for 28 days. EFFECT: higher antioxidant activity and decreased degree of radical product and lipid peroxide accumulation, in the absence of side and toxic effects of the plant tea, and applicability in experimental medicine and clinical practice. 4 tbl
The invention relates to medicine, in particular to pharmacology, can be used to stabilize the processes of lipid peroxidation in biomembranes and improving the antioxidant status of warm-blooded organism in conditions of exposure to ultraviolet rays and to find application in experimental medicine and clinical practice. Known methods for enhancing the antioxidant status of warm-blooded organism in the conditions of influence of prooxidant factors the introduction of synthetic drugs with antioxidant action - dibunola, tocopherol acetate, Mexidol [1], emoxipin [2] and the drug group of immunomodulators of plant origin Eleutherococcus [3]. The disadvantages of these methods are the need of the use of pharmacological drugs of synthetic origin, with a number of adverse and toxic effects and relatively high cost. A method of stabilizing processes peroxidation and increasing antioxidant status of warm-blooded organism in the conditions of UV exposure, which is a powerful Pro-oxidant factor, the introduction of infusion based herbal leaf, nettle, birch, plantain. Plants included in the collection, grow on the territory of our region (the Amur region) and are widely distributed on the territory of the Russia, contain a wide range of biologically active substances. Raw material used for manufacturing is available, the technology for cost-effective, wide range of applications. Infusion-based herbal no side or toxic effects, exerts a stabilizing influence on the processes of lipid peroxidation of membranes, increases the activity of the antioxidant system of the organism, providing, thus, the multidimensional positive influence on the organism of animals and humans in conditions of UV exposure and exposure to other prooxidant factors. The technical result of the invention to provide infusion based herbal plants (nettle leaves, birch, plantain), growing on the territory of our region (the Amur region) and its application to stabilization processes peroxidation and increasing antioxidant status of warm-blooded organism in conditions of ultraviolet radiation. The method consists in the fact that the experimental animals (rats or mice)under standard vivarium conditions for 20 minutes before irradiation in a UV chamber (Patent RF №2348079 from 27.02.2009 g) administered daily oral infusion of nettle leaves, birch, plantain at a rate of 5 ml/kg of body weight for 28 days. The infusion based on the collection of nettle leaves, Erez, plantain. Nettle leaves (harvested during flowering), birch (harvested in may), plantain (harvested in June - July) were crushed, mixed at the rate of 1:1:1, poured boiling water at a rate of 8 g per 200 ml of water, insisted 60 minutes, filtered, the precipitate was removed, the flask was cooled. On the 29th day of the experiment the animals were killed by decapitate. The results were taken into account by the ratio of LPO products (lipid hydroperoxides, diene conjugates, malonic dialdehyde) and the major components of AOC (ceruloplasmin, vitamin E) in the blood and internal organs compared to control animals, treated by statistical methods using the criterion of Wilcoxon signed-Mann-Whitney. The method allows for the increase of antioxidant activity and reducing the degree of accumulation of the products of radical and lipid peroxides in the body irradiated RIP mice and rats without the use of pharmacological drugs of synthetic origin, with a number of adverse and toxic effects and relatively high cost. Investigated the content of products of lipid peroxidation (lipid hydroperoxides, diene conjugates, malonic dialdehyde) and the major components of AOC (ceruloplasmin, vitamin E) in plasma and liver tissue of the control and the experimental groups (table. 1, 2, 3, 4).
As a result of the research the content of lipid hydroperoxides in the blood of animals treated on the background exposure infusion based on the collection of nettle leaves, birch and plantain, significantly lower by 32.4%, than in the control (exposed) group of animals (p<0,05), diene conjugates - by 34.8% (p<0.05), and malondialdehyde by 10%.
In the blood of experimental animals the content of ceruloplasmin significantly higher 34.3% compared with the control group rats (p<0.05)and the level of vitamin E - 24.3% (p<0,05).
In the liver tissue of experimental groups of animals, the content of lipid hydroperoxides was significantly lower by 23.9% compared with controls (p<0,05), diene conjugates by 36% (p<0.05), and malondialdehyde - 34.1% (p<0,05).
The content of ceruloplasmin in the liver tissue of experimental the Noah group of animals was significantly higher by 30.6% compared with controls (p< 0.05), and levels of vitamin E by 31% (p<0,05). INFORMATION SOURCES 1. Mashkovsky PPM Drugs: a Handbook for physicians. - M.: Medicine, 2004 - 685 S. 2. Dorovskikh VA Antioxidants in the prevention and correction of cold stress [Text] /Wagrowski, Eouropean, San. - Blagoveshchensk, 2000. - 183 C. 3. Dorovskikh VA Adaptogens and cold stress: yesterday, today, tomorrow...: monograph /Wagrowski, Nevsimalova, Nperson etc. - Blagoveshchensk: Dallgow, 2006. - 214 S. A method of improving the antioxidant status of warm-blooded organism to the stabilization of processes of lipid peroxidation of membranes under conditions of UV exposure of laboratory animals, characterized in that the experimental animals for 20 minutes before exposure to ultraviolet rays administered orally infusion based on the collection of nettle leaves, birch, plantain, taken in the ratio 1:1:1, and the resulting infusion of 8 g of the collection in 200 ml of boiling water, with the introduction of infusion carry out daily at a rate of 5 ml/kg of body weight for 28 days.
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