Method of treating rats with acute verapamil intoxications

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to toxicology, and can be used for treating rats with acute verapamil intoxications. That is ensured by administering sodium thiosulphate 15-20 mg per 100 g of rat's weights as a cardioprotector.

EFFECT: method provides improved central venous pressure value.

3 ex, 1 tbl

 

The invention relates to medicine, namely to toxicology, and can be used in the clinic for the treatment of patients with poisoning verapamil.

Acute chemical poisoning according to Basellandschaft are 3-4 overall number of cases and on the 1st place in absolute number of deaths (95040 people), exceeding the figure 2 and 3 times for tumors and myocardial infarction, respectively, and 13% for cerebrovascular diseases (Courland B.A. Chemical security of Russia in the light of the objectives of SES // Toxicological Bulletin. - 2007. No. 6. - P.8-10).

The main types of drugs most commonly cause acute poisoning, are considered blockers slow calcium channels (Savina A.S. Acute heart failure and its treatment in case of poisoning cardiotoxic poisons // Cardiology. - 1998. No. 4. - P.67-71).

Known application for the correction of cardio - and hemodynamics in acute poisoning verapamil calcium gluconate (cardioprotector - a substance that improves the metabolism of calcium in the myocardium), administered intravenously in a dose of 2-3 g (Vietemese Reference of clinical pharmacology of cardiovascular drugs (verapamil). - 2nd ed., revised and enlarged extra - M.: Publishing house of the BINOMIAL - SPb.: Nevsky Dialect, 2002. - 926 S.; Vietemese Arrhythmogenic effects of medical environments, the TV // Experimental and clinical pharmacology. - 2005. No. 2. - S-77.; Denisova I.N., Kulakov V.I., Haitov P.M. Clinical guidelines based on evidence-based medicine. - M.: GEOTAR-MED, 2001. - S. 1248 (section acute poisoning).

However, this treatment is sometimes not effective enough.

Also for the correction kardiogemodinamicheskuyu disorders known to use 10-20 ml of 10%calcium (Iagno, Clinical toxicology. M.: Medicine, 2008. s).

This treatment increases the level of calcium in the blood. But still the dosage of calcium in the administration of verapamil is controversial both in experiment and clinic (Li Saw Her F.L. Case report: Fatal verapamil overdosage despite intensive therapu and use of high dose intravenous calcium // J Hum Hypertens. - 1996. No. 10 (7). - P.495-496; Calcium channel blocking drug overdose: an Australien series / D.M.Howarth, A.N.Dawson, A.J.Smith et al. // Hum Exp Toxicol. - 1994. No. 13 (3). - P.161-166; Calcium and digoxin vs. calcium alone for severe verapamil toxicity / T.C.Bania, B.Blaufeux, S.Hughes et al. // Vcad Emerg Med. - 2000. No. 7 (10). - P.1089-1096.) and even when reaching the highest numbers in the blood does not lead to stabilization of cardio - and hemodynamics. Introduction calcium is only effective when reduced heart rate (HR).

Object of the invention is the recovery of myocardial function and blood flow in rats with poisoning verapamil.

The technical result that will be achieved from the use of the invention is to improve hemodynamic pok the point, Central venous pressure (CVP).

The technical result is achieved by the fact that treatment of rats with acute poisoning verapamil carried out by introducing cardioprotector - sodium thiosulfate at a dose of 15-20 mg per 100 g of rat.

The invention consists in the elaboration of the methods of medicamentous correction of the degree of violation of the Central venous pressure verapamil.

The importance of the Central venous pressure, as one of the parameters of Central hemodynamics, reflected in the clinical literature (Bdikin, Avista. Monitoring respiration and hemodynamics in critical States. - Ekaterinburg: Socrates, 2006. - S.257). In the experimental literature (basic parameters and constants in animals), this figure is not reflected, hence the importance of his analysis with the introduction of toxic substances and drug administration (Performance standards in laboratory animals in Toxicological experiment (modern concepts and methodological approaches, the main parameters and constants) / Imeretina, Resava, Whosever and others - M.: Medicine, 1978. - P.59; the Problem of standards in toxicology / Imeretina, Resava, Whosever, Paganiniana. - M.: Medicine, 1991. - P.59).

The introduction of sodium thiosulfate for the treatment of rats with acute poisoning verapamil still not used.

Dosage of 15-20, MGN 100 g of rat we have selected eksperimentalno and is optimal.

This dosage allows to achieve a positive impact as a cardioprotective drug to stabilize the membranes of cardiomyocytes - the protection of sulfhydryl groups of enzymes from oxidation and preservation of their activity, prevention actions ischemic toxin in membranes of mitochondria and stabilization of the cell membrane and decrease the level of processes of lipid peroxidation.

The invention is carried out as follows.

One group of rats (Mature white outbred rats male weighing 20010 g) (10 PCs) previously injected intraperitoneally verapamil at a dose of 2.5 mg/100 g of rat.

The second group of rats (10 units) previously injected intraperitoneally verapamil at a dose of 2.5 mg/100 g of rat. Then in the 20th minute after administration of verapamil was administered sodium thiosulfate at a dose of 15-20 mg per 100 g of rat in the right jugular vein.

In these groups underwent measurement of initial Central venous pressure (mm vods) and pressure at the 5th, 10th, 20th, 30th, 40th, 50th and 60th minute.

The duration of experimental poisoning accounted for 60 min and was selected to account for the circulation of verapamil in the blood of experimental animals and humans and the ability of drugs to cause significant violations of cardio - and hemodynamics in acute poisoning (Gallenkamp, Medical toxico age, 2003 - S-568).

The selected dose of verapamil were taken from literature sources (Neuropharmacological study of the effects of joint injection verapamil and olanzapine on the content of neurotransmitters in the brain structures of rats Wistar / Vssadmin, Pimlott, Webarchives etc. // Experimental and clinical pharmacology. - 2005. No. 6. - P.12-14. Cherednik I. Comparative evaluation of antiarrhythmic actions of novocained and isoptin with neurogenic atrial fibrillation // Abstracts of the 5th Grew. the NAC. fo. "Man and medicine". - M., 1998. - S.) and adapted by us for our methodology, intraperitoneal conduct, in connection with the need for more accurate dosage injection verapamil, simplicity and less traumatic for the animal.

The invention is illustrated by the following examples of specific performance:

The group with acute poisoning verapamil

Example 1. Rat male weighing 200 g were injected intraperitoneally verapamil at a dose of 2.5 mg/100 g in mass. Measurement of initial Central venous pressure and the pressure at the 5th, 10th, 20th, 30th, 40th, 50th and 60th minute measured by cateora introduced into the right jugular vein. In the 60th minute poisoning the value of CVP amounted to - 1.1 mm Vogt

Example 2. Rat male weighing 190 g were injected intraperitoneally verapamil at a dose of 2.5 mg/100 g in mass. Measurement of initial Central venous pressure and pressure the Oia on the 5th, The 10th, 20th, 30th, 40th, 50th and 60th minute measured by cateora introduced into the right jugular vein. The value of the CVP in the 60th minute was - 1.0 mm Vogt

Example 3. Rat male weighing 210 g were injected intraperitoneally verapamil at a dose of 2.5 mg/100 g in mass. Measurement of initial Central venous pressure and the pressure at the 5th, 10th, 20th, 30th, 40th, 50th and 60th minute measured by cateora introduced into the right jugular vein.

The value of the CVP in the 60th minute was - 1.4 mm Vogt

The group with the introduction of sodium thiosulfate.

Example 1. Rat male weighing 190 g were injected intraperitoneally verapamil at a dose of 2.5 mg/100 g in weight followed by the introduction of sodium thiosulfate and 20 min at a dose of 18 mg/100 g mass. Measurement of initial Central venous pressure and the pressure at the 5th, 10th, 20th, 30th, 40th, 50th and 60th minute measured by cateora introduced into the right jugular vein. The value of the CVP in the 60th minute of the poisoning amounted to - 3.1 mm Vogt

Example 2. Rat male weighing 200 g were injected intraperitoneally verapamil at a dose of 2.5 mg/100 g in weight followed by the introduction of sodium thiosulfate and 20 min at a dose of 20 mg/100 g mass. Measurement of initial Central venous pressure and the pressure at the 5th, 10th, 20th, 30th, 40th, 50th and 60th minute measured by cateora introduced into the right jugular vein.

The value of the CVP in the 60th minute after the poisoning amounted to - 3.5 mm Vogt

Example 3. Rat male weighing 200 g intraperitoneally who drove verapamil at a dose of 2.5 mg/100 g in weight followed by the introduction of sodium thiosulfate and 20 min at a dose of 15 mg/100 g mass. Measurement of initial Central venous pressure and the pressure at the 5th, 10th, 20th, 30th, 40th, 50th and 60th minute measured by cateora introduced into the right jugular vein.

The value of the CVP in the 60th minute amounted to 3,2 mm Vogt

Techniques for performing other similar examples are given.

The change in Central venous pressure in acute poisoning verapamil and poisoning with medication adjustments shown in the table.

Table
The change in Central venous pressure in acute poisoning verapamil and poisoning with medicamental correction (Mm)
The stages of the experimentGroups of ratsCVP mm Vogt
ExIn6,10,2
In+T6,20,2
Dynamics of acute poisoning
5 minIn2,40,1
In+T2,50,1
10 minIn 2,20,2
In+T2,20,1
20 minIn1,80,2
In+T1,90,1
30 minIn1,60,1
In+T2,10,1
40 minIn1,40,2
In+T2,40,1
50 minIn1,20,2
In+T2,50,1
60 minIn1,00,1
B+T (15 mg)3,10,1
(18 mg)3,10,2
(20 mg)3,60,2
Note. In groups of introduced rats with verapamil.
B+T - a group of rats with verapamil+sodium thiosulfate

As can be seen from the above data, h is Sova dynamics of Central venous pressure in acute poisoning verapamil was characterized by a progressive decrease Central venous pressure. Data source and 60th minutes of poisoning.

Sourceof 6.10.2 mm Vogt
CVP at the 60th min poisoningto 1.00.1 mm Vogt

As can be seen from the above data, there is a significant change in the level.

Considering the experimental results of the dynamics of venous pressure, you can make the following conclusion: the introduction of sodium thiosulfate in the early stages (20 min) helps prevent the development of more severe degree of disorders of the Central venous pressure, thereby improving the state of the experimental animal is a rat.

Thus, this method allows high accuracy to evaluate the influence of the drug and its dose on the degree of change in Central venous pressure in acute poisoning verapamil.

Treatment of rats with acute poisoning verapamil by introducing cardioprotector, characterized in that as cardioprotector injected sodium thiosulfate at a dose of 15-20 mg per 100 g of rat.



 

Same patents:

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to zinc-containing antidote of lethal and severe poisonings with ethanol. Claimed antidote represents intramolecular tricyclic complex of triethanolamine with zinc salts of inorganic or organic acids (2, 8, 9-trihydrozincatran), the ratio of triethanolamine to zinc salts being 1:1. Invention also relates to method of treating ethanol poisoning by introduction into organism of zinc-containing antidote in 5 vol.% ethanol in dose range 30-60 mg/kg of body weight.

EFFECT: prevention of lethal outcome in case of ethanol poisoning and reduction of acute toxicity of zinc-containing antidote.

9 cl, 5 ex

FIELD: chemistry.

SUBSTANCE: Chinese magnolia is extracted with liquefied carbon dioxide. The obtained CO2 extract undergoes chromatographic separation on aluminium oxide and eluated with hexane. After eluation the obtained hexane fractions are frozen and recrystallised from the chloroform-hexane mixture in ratio of 1:10-3:10.

EFFECT: high output of product.

1 ex

FIELD: medicine; veterinary science.

SUBSTANCE: method consists in introduction to an experimental animal of Acyzol 30 mg/kg once a day combined with daily subcutaneous introduction of cadmium sulphate solution in a dose 0.1 mg/kg.

EFFECT: prevention of cadmium toxic effect in chronic poisoning in experimental animals.

2 cl, 1 tbl, 1 ex

Medicinal agent // 2363463

FIELD: medicine.

SUBSTANCE: invention relates to medicine and pharmaceutical industry, and more specifically to making tableted dosage form with prophylactic effect for arresting primed reaction to radiation and early transient incapacity. The agent contains ondansetron, methacin and caffeine in weight ratio ondansetron:methacin:caffeine 4:1:20-300.

EFFECT: reduced adverse reaction, more specifically vomiting, diarrhea and reduction of physical activity.

5 cl, 11 tbl

FIELD: medicine.

SUBSTANCE: claimed is remedy possessing antitoxic properties in case of aliphatic alcohol poisoning. As such polyosm - 30% solution of polyethylene oxide 400 is suggested. It is shown that polyosm protects animals against death (100% in control) in case of isopropyl alcohol poisoning.

EFFECT: water content in lungs is analogous to index of intact animals at moderate reduction of respiration rate.

6 tbl, 9 ex

FIELD: chemistry.

SUBSTANCE: invention refers to veterinary science, in particular to products intended to increase capacity for survival and yield of poultry. For this purpose common composition 1,3-di(3-piperidine-2-hydroxypropyl)-6-methyluracil of formula: is used. Composition is characterised by natural resistance, antioxidant activity, activates nonspecific factors of immune system, as well as possesses medicinal properties to provide detoxification of nitrates and nitrites. Composition has the appearance of white powder, is highly soluble in water and can be added in specified doses to chickenfeed.

EFFECT: production of composition providing increase of capacity for survival and yield of poultry.

6 tbl, 5 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to pharmaceutical industry and deals with acizol application as preventive and therapeutic means in neurotoxin poisoning cases. The present invention showed high efficacy of acizol in complex treatment for toxico-hypoxic encephalopathy and pneumonia (normalisation of homeostasis indices, lowering of mortality and disabling complications).

EFFECT: lowering of the risk of complications in neurotoxin poisoning cases.

15 tbl

FIELD: medicine; pharmacology.

SUBSTANCE: wood of Siberian or Daurian larch is debarked, cleaved and dried at 40-50°C to residual moisture 23-27%. Dried wood is chopped, and soluble substances are extracted with 75-85% ethyl alcohol aqueous solution at temperature 45-50°C in ratio raw material:extracting agent 1:(7-10). Further extracting agent is distilled off, and sawdust is supplied to press for additional alcohol return. Then extract aqueous part is cooled to 20-25°C within 20-30 minutes for isolation of resinous impurity accompanying dihydroquercetin (DHQ). Deresined extract aqueous part is added with methyltertbutyl ether (MTBE) in ratio 1: (0.3-0.45), and DHQ is reextracted within 2-3 hours. Extract ether part is isolated separate from aqueous part through sedimentation within 2-2.5 hours and supplied to MTBE evaporation, while target product is crystallised of hot water. Invention enables to produce DHQ with yield 2.2-2.5% of bone-dry wood mass with grade 90-96%.

EFFECT: simplification of process and production of high-quality product.

4 cl, 2 ex

FIELD: medicine; veterinary.

SUBSTANCE: method describes the use of 2,4-diphenyl-7,8-benzo-5,6-dihydroselenochromen as a means of treatment and prevention of poisoning with heavy metal compounds.

EFFECT: higher resistance in animals and humans to poisoning with heavy-metal compounds.

8 tbl, 1 ex

FIELD: organic chemistry, toxicology.

SUBSTANCE: invention describes a method for using 2,4,6-triphenyl-4H-selenopyrane as an agent for treatment and prophylaxis of poisoning with arsenic compounds. Sodium arsenite was used as a toxicant. Using the claimed preparation reduces lethality of animals up to 40-60% (100% in control group). Also, significant improving blood indices and visceral organs of animals occurred, i. e. the severity degree of poisoning was decreased.

EFFECT: enhanced effectiveness of agent.

2 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to chemical-pharmaceutical industry, namely to creation of composition of dense extract of conifer needles for capsulation. Composition of dense extract of conifer needles contains the following ingredients: dense extract of conifer needles, emulsifier, a-tocopherol (vitamin E), retinol (vitamin A), naphthoquinones (vitamin K), essential fatty acids (vitamin F), vegetable oil. Microelements can be included additionally.

EFFECT: composition improves state of mucous membranes and enhances protective properties of organism.

2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to chemical-pharmaceutical industry and medicine and deals with composition for parenteral introduction, which contains (wt %): 2-ethyl-6-methyl-3-hydroxypyridine succinate as active component - 4.0-6.0; stabiliser - sodium metabisulfite - 0.015-0.045 and water as solvent - to 100. Claimed invention also relates to method of obtaining said composition. Composition is characterised by stability during three years, low toxicity, comparable with 2-ethyl-6-methyl-3-hydroxypyridine succinate solution without stabilisers, makes it possible to realise therapeutic action of active substance without undesirable side pharmacologic activity of auxiliary substances.

EFFECT: invention can be used in medicine as medication for treatment and prevention of various diseases and pathological states of human organism.

3 cl, 6 ex, 2 tbl

FIELD: medicine.

SUBSTANCE: invention relates to field of veterinary. Method consists in the following: applied is "EVL-Se-COMPOSITION" solution which is introduced into animals by group method with water one time per day in single dose 0.05 ml during 10 days, 5 days before weaning and 5 days after weaning.

EFFECT: application of claimed method makes it possible to realise prophylaxis of gastrointestinal diseases, increase average daily weight gain, does not cause side effects.

3 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to dermatology and can be applied for eczema treatment. For this purpose additionally to complex drug therapy administered is seramil intramuscularly in dose 0.5 mg every second day. Treatment course consists of 10 days.

EFFECT: method provides effective treatment, absence of side effects, reduction of recurrence number, stable remission, accompanied by normalisation of immune system and reduction of treatment terms.

3 ex

FIELD: medicine, veterinary science.

SUBSTANCE: group of inventions concerns veterinary science, particularly, methods and medicinal agents for treating parasitic diseases in waterfowl. The method of treating involves introduction of a medicinal agent. As a medicinal agent, a tablet 0.2 g is introduced orally to waterfowl in dosage 1 tablet per 7 kg of bird's weight. And the agent contains praziquantel, ivermectine, sodium selenite and target additives in the following amount (g in 1 tablet): praziquantel - 0.035, ivermectin - 0.0014, sodium selenite - 0.0005, target additive - 0.1631.

EFFECT: method and agent exhibit high therapeutic antiparasitic efficacy and lowered toxicity.

6 cl, 5 ex

FIELD: medicine, veterinary science.

SUBSTANCE: group of inventions concerns veterinary science, particularly, methods and medicinal agents for treating parasitic diseases in waterfowl. The method of treating involves introduction of a medicinal agent. As a medicinal agent, a suspension in introduced to waterfowl in a single dose 1 ml of the suspension per 1 kg of bird's weight. The agent contains praziquantel, ivermectin, sodium selenite, nipagin, nipasol, lactose, Tween 80, gelatin and distilled water in the following ratio of components (wt %): praziquantel - 0.5, ivermectin - 0.02, sodium selenite - 0.0071, nipagin - 0.01, nipasol - 0.005, lactose - 30.0, Tween 80 - 2.0, gelatin - 2.0, distilled water - the rest.

EFFECT: method and agent exhibit high therapeutic antiparasitic efficacy and lowered toxicity.

3 cl, 5 ex

FIELD: medicine, veterinary science.

SUBSTANCE: invention relates to veterinary science, namely to stress correctors and adaptogens for animals. Preparation for correction contains selenium in nanosize condition and zero valency (Se0) , ascorbic acid and water for injections, at the following ratio of components in wt %: selenium (Se0) 0.001-1.0; ascorbic acid 5.0-20.0; water for injections - balance.

EFFECT: invention provides for reduction of toxicity, high availability for organism, higher antioxidant and anti-stress effect of agent, lower costs for its production.

2 tbl, 4 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to pharmacy, namely to creation of complexes for treatment of chronic fatigue syndrome (CFS). Four naturopathic complexes for CFS treatment contain herbal mixtures and homeopathic medications.

EFFECT: intake of naturopathic complexes ensures high therapeutic effect throughout the treatment without negative side effects and disease recurrences.

5 cl, 4 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to pharmaceutical industry, in particular to anti-inflammatory composition. Method of reducing skin irritation, which includes contact of skin, displaying irritation, with preparation of personal hygiene, including composition with anti-inflammatory properties which contains (a) anti-inflammatory substance, representing extract of olive leaves (Olea Europea) and (b) anti-inflammatory agent, containing combination of sodium cocoyl amino acids, magnesium aspartate and potassium aspartate, ingredient a) and ingredient b) being represented in said composition in specified ratio (versions).

EFFECT: medications reduce skin irritation efficiently, possess synergetic anti-inflammatory effect.

12 cl, 4 ex

FIELD: medicine, veterinary.

SUBSTANCE: invention relates to farm animal feeding. Method lies in the following: to fed full cream milk or in full cream milk substitute of standard recipe introduced is selenised girasol in amount 50-70 grams per one calf per day from 5-day age for 35 days.

EFFECT: method allows to increase natural resistance of calves fed with milk and amplifies in their organism synthesis of antibodies belonging to immunoglobulins of class G.

1 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: the present innovation includes synthetic zeolite Na-A, sulfur and flavoring at the following ratio of components, weight%: sulfur 0.03-0.08, flavoring 1.0-1.5, synthetic zeolite Na-A - the rest. As flavoring one should apply food flavorings with the odor of vanillin, lemon, prune, almond, coffee. Organoleptic evaluation of preparation's properties has shown that it causes no irritation of human hand skin: hands become dry and demonstrate pleasant odor. Due to supplementing sulfur the suggested preparation provides elimination of harmful microflora, protects hand skin against infection. Preparation is simple to be manufactured and applied along with its low price and ecological safety.

EFFECT: higher efficiency.

1 cl, 1 tbl

Up!