Diagnostic technique for vibration sickness

FIELD: medicine.

SUBSTANCE: venous blood is taken from a worker exposed to vibration action to analyse a neuron-specific enolase (NSE) level by immune-enzyme assay (IEA). If the NSE concentration is less than 20 ng/ml, the absence of vibration sickness in the person being tested is stated, the NSE level being within 20 to 30 ng/ml shows the presence of degree 1 vibration sickness, while the NSE level exceeding 30 ng/ml indicates degree 2 vibration sickness.

EFFECT: simplified diagnosing of vibration sickness and evaluation of a manifestation degree of vibration sickness.

1 tbl, 3 ex


The present invention relates to medicine, namely, neurology and pathology, and is intended for diagnostics of vibration disease (WB).

A known method for the diagnosis of WB (Lyubimova R.P. "the Dynamics of clinical and electrophysiological changes in the neuromuscular system of patients with vibration disease", journal of Neuropathology and psychiatry, 1991, No. 9, s-17), based on the collection of complaints, the investigation of neurological status, biochemical blood analysis (including determination of calcium content), identifying angiospastic disorders, disorders of pain and vibration sensitivity, needle electromyography (EMG). To substantiate the diagnosis WB Replusive recommends that in the analysis of EMG to estimate the duration of motor unit potentials (PDE), maximum and average amplitude of PDE, the degree of deviation of the characteristics of the histogram of biopotentials from age norms, the severity fibrillyatsy and fasciculations.

Closest to the present invention is a method for the diagnosis of vibration disease (Occupational diseases. A guide for physicians. M: Medicine. 1996 edited Nefazodone, in 2 volumes. Volume 2, Chapter 7. Vibration disease. S-162.), based on a comprehensive examination of the patient, including complaints, clinical and laboratory data, as well as functional is s research methods (cold test, skin thermometry, altimetry, the determination of the threshold sensitivity vibration, dynamometry, rheovasography, stimulation electroneuromyography, electroencephalography), aimed at identifying the major syndromes WB - peripheral angiopathies syndrome (white finger) and syndrome of vegetative-sensory polyneuropathy, which are found in patients with WB, both the first and second degrees, only more pronounced (Occupational diseases. A guide for physicians. M: Medicine. 1996 edited Nefazodone, in 2 volumes. Volume 2, Chapter 7. Vibration disease. P.146-147). A disadvantage of the known methods of diagnosis WB is a subjective approach to the refinement stage of development of the WB to the patient due to the lack of precise quantitative criteria; it is a costly and time-consuming procedure involving the use of a large number of different methods.

The objective of the invention is a simplified method for the diagnosis of vibration disease without reducing the quality of diagnosis, quantifying the severity of vibration disease.

The task is solved in that in the method for the diagnosis of vibration disease, including assessment of clinical signs, carry out the determination of the level neurospecific enolase (NSE) in serum by the method of immunopharm what these assay (ELISA), and when the content of NSE less than 20 ng/ml make a conclusion about the absence of the subject of vibration disease, when NSE from 20 to 30 ng/ml - about the presence of vibration disease first-degree gravity, with NSE more than 30 ng/ml - second vibration disease severity.

In the clinic, Yekaterinburg medical research center surveyed 167 miners vibration threatening professions, whose average age was 45.6±0,6 years, the average work experience is 19.2±0.8 years. For the purpose of diagnosis of vibration disease all work the survey was conducted according to the standard technique (Occupational diseases. A guide for physicians. M: Medicine. 1996 edited Nefazodone, in 2 volumes. Volume 2, Chapter 7. Vibration disease. S-156), and determined the content of NSE in serum by ELISA.

After the inspection and identify the business objective changes (symptom PAL, a symptom of "white spots", gipestesia), and data of instrumental study (conducted: palleschisommese to determine threshold sensitivity vibration; electroneuromyography (ENMG) of the upper extremities with the speed of conduction of the impulse (IPN) touch (Spies) and motor (Sleep) the median and ulnar nerves, and terminal latency (TL) and the amplitude of the M-response of the same nerves; according to the days (RVG) of the forearms and wrists were analyzed pulse volume (RI) vessels and the coefficient asymmetry blood; all work is remodels cold test), decisions of the expert Commission on the communication of disease and occupation of the surveyed workers were divided into 3 groups: the first group - patients with vibration disease first severity (VB) - 47 (28%), work experience with Vibroscreen - 23,0±0.6 years; the second group included patients with vibration disease in the second degree of severity (VB) - 62 (37%), work experience - 23,9±0.5 years. The third group was made up of working groups risk for the development of vibration disease (58 people, 35% of those surveyed), the average length of which was 6.0±0.5 years. All examined patients WB was diagnosed with the syndrome of vegetative-sensory polyneuropathy of upper extremities.

Comparison of two methods to assess the severity of the manifestations of vibration disease (as defined by the level of NSE and generally accepted criteria for the diagnosis of vibration disease using instrumental methods) showed that the claimed method is not inferior to the prototype for the quality assessment of the extent WB (table 1).

The data obtained showed that the performance of instrumental methods of research workers risk group did not differ from those of healthy persons diagnosed only (p<0.001) and the asymmetry of the pulse of the blood vessels of the hands. The level of concentration of NSE in serum CROs and the average for the group amounted to 13,0±6,6 ng/ml and did not differ from that of healthy persons.

Patients VB was registered slow SLEEP (p<0,001), both sensory and motor fibers of the median and ulnar nerves and increase terminal latency (in comparison with the group of healthy individuals), indicating that the development of autonomic-sensory polyneuropathy of upper extremities. According to the RVG marked tendency to decrease and the asymmetry of the pulse of the blood vessels of the hands. The threshold vibration sensitivity was significantly (p<0,001) higher than in healthy persons. in Addition, all indicators analyzed in patients VB significantly (p<0.001) and differed from those of workers at risk. The concentration of NSE in serum of patients VB 23.4±4.5 ng/ml and was significantly higher (p<0,001) indicators, as healthy individuals, and workers at risk.

Indicators ENMG (Spies, Sleep, TL, both the median and ulnar nerves) in patients VB, as well as in patients VB, had no significant difference (p<0,001) with the same values in healthy persons In the study of vibration sensitivity registered an increase (p<0,001) threshold sensitivity vibration. At the same time, according to ENMG we did not reveal significant differences between groups of patients VB and VB marked only the tendency to slow down Spies and increase t as the middle is my, and ulnar nerves. The concentration of NSE in serum was 44.5±8.7 ng/ml and was significantly (p<0.001) above indicators as healthy individuals and patients VB.

The authors believe that NSE is a highly specific marker of neurons that perform multiple functions, including those involved in myelinopathy [Poletaev A.B. Meshoperations group proteins S-100, their endogenous acceptors and ligands and the regulation of metabolic processes in the nervous tissue: author. Diss. ... Prof. The honey. Sciences. - M., 1987. - P.20-23.; Abramov V.V. Integration of immune and nervous systems // Novosibirsk: Nauka, Siberian branch, 1991. - 168 C.]. There are many works devoted to the role neurospecific proteins in neurodegenerative diseases of the CNS [Belyaev I.A. Neurospecific proteins in blood and spinal fluid with tick-borne CNS (clinical, diagnostic and prognostic aspects): author. discard. the honey. Sciences. - M., 1995. - 22 S.; Nagdyman N., Grimmer I., Scholz T. Predictiv value of brain-specific proteins in serum for neurodevelopmental outcome after birth asphyxia // Pediatr. Res. - 2003. - Vol.54(2). - P.270-275.). Obtained data show that in vibration disease level of NSE in serum significantly increased, therefore, the NSE can be considered a marker of disorders of the peripheral nervous system.

The method is as follows.

The worker exposed to in the brazii, take blood from a vein and determine the level of NSE enzyme-linked immunosorbent assay (ELISA) using kits "MAGIWEL (USA) according to the following scheme: dispense 25 ál of NSE standards and patient samples in cells in duplicate, make 100 μl incubation solution in each cell, without touching the surface of the liquid; incubate 1 hour at room temperature (20-28°C) with constant stirring die on the shaker for microplates, after incubation, remove the liquid and wash each strip 5 times, making each cell 100 ál of enzyme, incubated 60 minutes at room temperature, remove the mixture and washed 5 times with buffer, incubated for 30 minutes at room temperature with constant stirring die on the shaker, avoiding direct sunlight. The optical density immediately measured on a plate reader at 450 nm. Each measurement standards and patient samples are carried out in two takes. Before using all samples and reagents are brought to room temperature (24,3±3°C). Then largest NSE determine the severity of vibration disease: when the content NSE less than 20 ng/ml make a conclusion about the absence of the subject of vibration disease, NSE from 20 to 30 ng/ml - about the presence of vibration disease first-degree gravity, with NSE more than 30 ng/ml - second vibration disease severity.

Example No. 1. Patient I., miner, St the W work with Vibroscreen 23 years. Was admitted to the neurology clinic with complaints of numbness, sensitivity to cold and paresthesias in the hands, whitening of the terminal phalanges 4 and 5 fingers on the cold, convulsive tightening and reduction of strength in the hands, sleep disturbance because of aching pain in the hands, shortness of breath, fatigue, irritability.

Objective examination: tenderness to palpation of namyslow shoulders and shoulder-beam muscles, especially in the middle third. Brush bluish-hypermonogenic, wet, reduced ripple on a.radialis with two sides, a symptom of "white spots" 7 seconds symptom PAL positive from both sides. Hypoesthesia on the hands on polyneuritic type. Tendon reflexes of the upper and lower extremities symmetric, quickened. According to ENMG upper limbs showed signs of polyneuropathy: a decrease in the rate of impulses in sensory (List) fibers of the median nerve (45 m/s), amplitude of the M-response (5,9 MB) and a slow terminal (TL) latency (3 msec). In the study of the ulnar nerve Spies was 45 m/sec, Sleep - 56 m/s, the amplitude of the M-response - to 5.4 mV, t is 2.5 msec. The threshold of occurrence of the M-response was 7 mA. The initial skin temperature in the area of the nail phalanx of the third finger of the right hand was 30,9°C, after the cold test and 22.8°C (test positive). On RVG upper extremities reported to decrease blood (RI - 1,02$) and signs of increasing vascular tone hands. The threshold vibration sensitivity was increased and amounted to 118%. On radiographs of the elbow and shoulder joints, osteo-articular pathology was not detected. On the basis of complaints, physical examination data, and instrumental methods of examination, was diagnosed with vibration disease first the severity of the syndrome of vegetative-sensory polyneuropathy of upper extremities. The concentration of NSE in serum was registered at the level of 24.7 ng/ml, which confirms the above diagnosis in a given patient.

Example No. 2. The patient Including, the drifter, the experience of working with Vibroscreen 25 years. Was admitted with complaints of pain and convulsive contraction in the hands, pain in the shoulder and elbow joints, numbness of the fingers, "whitening" of the fingers, sleep disturbance, fatigue, pain in the cervical spine.

At objective examination: the brush of cool, wet, cyanotic, the symptom of "white spots" 8 seconds, reduced pulsation of the radial artery on both sides, hypesthesia on polyneuritic type type high glove to the level of the middle third of the forearm. Sharp pain on palpation of external namyslow shoulders. According to ENMG showed signs of polyneuropathy: a List of the median nerve was 45 m/s, the List of the ulnar nerve - 46 m/s. The temperature of the third finger on the right hand corresponded to 23.2°C, POS is f cold test - 23,5°C (sample indifferent). On RVG - pulse blood vessels in the area of the brushes significantly reduced (RI $ 0,68). On the basis of complaints, physical examination data, and instrumental methods of examination, diagnosis: second vibration disease severity, symptoms of autonomic-sensory polyneuropathy of upper extremities with angiospazmam fingers, outer and inner epicondyles shoulders, periastron shoulder joints. The concentration of NSE in serum was registered at the level of 67 ng/l, which confirms the above diagnosis in a given patient.

Example No. 3. Patient K., drifter, experience with Vibroscreen 10 years. Upon admission to the clinic complained of numbness and paresthesias of the tips of the fingers of the hands. At objective examination: the skin of normal color, hypesthesia on the fingers. According to RVG - pulse blood pressure forearm moderately reduced (RI - 0,75 USD), the asymmetry of the pulse of the blood vessels of the arms (28%). The temperature of the third finger brush 30°, after cold test - 28,7° (test positive). When ENMG of the median nerve Sleep was 55 m/sec (normal), List - 54 m/sec (normal), and in the study of the ulnar nerve - Sleep - 58 m/s, Spies - 51 m/s (in the range of normal values). When setting the threshold sensitivity vibration is not what Yavlena deviations from the norm. On radiographs of the cervical spine revealed a marginal osteophytes2-C4and the reduction of height of intervertebral discs. After the survey was diagnosed with osteochondrosis of the cervical spine. The concentration of NSE in serum registered at the level of 6.5 ng/l, which confirms the absence of the examined miner vibration disease.

The advantages of the proposed method for the diagnosis of vibration disease should include: a simplified method for the diagnosis of diseases and objective quantitative assessment of the identified changes.

A method for the diagnosis of vibration disease, including assessment of clinical signs, characterized in that they determine the level of neuron-specific enolase (NSE) in serum enzyme-linked immunosorbent assay and content NSE less than 20 ng/ml make a conclusion about the absence of the subject of vibration disease, NSE from 20 to 30 ng/ml - about the presence of vibration disease first-degree gravity, with NSE more than 30 ng/ml - second vibration disease severity.


Same patents:

FIELD: medicine.

SUBSTANCE: method of quantitative determination of human chitinase-like protein YKL-40 (another name - human cartilaginous glycoprotein-39, or HcGP-39) is offered. When implementing the method, colloidal chitin or heparin is sorbed in microplate wells, then solutions containing a certain amount of chitinase-like protein, and analysed samples are introduced in the wells. The amount of YKL-40 is analysed by the amount of an enzymatic reaction product formed by interaction of an enzymatic conjugate with YKL-40 antibodies against with a substratum of this enzyme.

EFFECT: method allows well reproducible and high sensitive YKL-40 analysis in various human biopsy materials and excretes.

2 cl, 2 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: once admitted to hospital, a patient suffering acute cholecystitis is examined for the blood serum lactoferrin level to calculate a range score; the serum lactoferrin values 200 ng/ml are taken as 1 point; respectively, the lactoferrin level 8 point and lower enable to observe a destructive form of chronic cholecystitis; the lactoferrin level within 8 to 10 points shows catarrhal cholecystitis, and the level 10 points and higher indicates destructive cholecystitis.

EFFECT: use of the technique allows higher diagnostic accuracy in gallbladder tissue destruction in associated acute cholecystitis.

3 ex

FIELD: medicine.

SUBSTANCE: method of determining individual human drug sensitivity or immunotoxicity is based on individual modelling in vitro of the effect on IFN-alpha and/or IFN-gamma production by patient's whole blood leukocytes/lymphocytes. The method involves evaluating a degree of a stimulating/correcting or inhibitory drug action and/or afteraction on the primary IFN system reactivity values.

EFFECT: method provides individual prescription of adequate drugs and thereby higher clinical efficacy for prevention of potential developing medicinal interferon immunodeficiencies.

3 cl, 4 ex

FIELD: medicine.

SUBSTANCE: for selective recovery of a viable cell population, a biological fluid sample is placed in a microfluid device cell containing a silicon microchannel matrix with through holes of size 3-30 mcm and channel length 50-300 mcm as a filter medium, and passed through the matrix at rate 0.1-4 ml/min. In case of cell size separation, a cell fraction of the size less than that of the through holes in the matrix, are collected after passing of the microchannel matrix, while the larger cells are eluated from the microchannel matrix channels by eluent backflow. In case of receptor-specific cell separation, a matrix surface is pre-modified by specific cell receptor antibodies, while the target cells are eluated from the matrix channels by isotypic antibodies or haptens.

EFFECT: more efficient and faster selective recovery of the viable cell population from biological fluids.

3 cl, 2 dwg, 4 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: for detecting thrombinemia associated with large joint replacement, on the 10th-14th postoperative day, patient's blood plasma is examined for the hemostasis system values: the concentrations of D-dimer, plasminogen and antithrombin III. A discriminator (Z) is calculated by formula: Z=4.3936-0.001×D-0.029×P+0.017×A, where D is the concentration of D-dimer (ng/ml FEU), P is the concentration of plasminogen (percentage of normal concentration), A is the concentration of antithrombin (percentage of normal concentration). If observing the value Z <0, high probability of preserving thrombinemia is diagnosed, and pharmacological antithrombotic prevention is recommended to be continued.

EFFECT: use of the declared method allows high-efficient determination of appropriateness of prolongation of a pharmacological antithrombotic prevention course following the large joint replacement.

2 ex

FIELD: medicine.

SUBSTANCE: invention relates to biology and medicine, namely to experimental models of immunologic states, and can be used for estimation of anti-ergotypic response to vaccination with polyclonally activated cells. For this purpose vaccination with splenocytes, activated by means of anti-CD3 antibodies and interleukin-2, by subcutaneous injections 1 time per week during 4 weeks. After that response of delayed-type hypersensitivity (DTH) is induced by introduction of activated splenocytes under aponeurosis of animal paw pad. Anti-ergotypic response is estimated by degree of DTH development.

EFFECT: implementation of method provides possibility of experimental reliable estimation of anti-ergotypic response in vivo, induced by polyclonally activated cells, and estimate its connection with changes of autoimmune disease course, performing control over autoimmune responses.

1 dwg, 2 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: substance of the invention involves H.capsulatum G18 5 P cultivation in a mycelial growth phase on a Smith synthetic protein-free salt medium at temperature 28°C for 3 months then a biomass is separated by bulky filtration and sterilised through membrane filters that is followed by a sterility test, and then the antigens found in the culture medium are precipitated with acetone cooled to -20°C and dried. The erythrocytic diagnostic antigen is produced on the recovered antigen complex common for histoplasmosis and coccidioidomycosis.

EFFECT: higher stability and indirect hemagglutination reproducibility.

2 cl, 3 ex, 2 tbl

FIELD: medicine.

SUBSTANCE: analysis for human immune status disorder is enabled by sheep's erythrocyte lysis with 25 % human blood serum with using endogenous heterophil antibodies at temperature (37.0±0.5)°C for 10 minutes with 0.288M NaCl added in a buffer solution. If observing erythrocyte lysis inhibition less than 35 %, human immune hyperreaction is presented, 35-70 % shows a normal reaction, more than 70% - lower reaction.

EFFECT: more efficient detection of preclinical immunodeficiency conditions, enabled in-depth examination of the pathogenesis of autoimmune diseases, and early prevention, controlled efficacy of a conducted therapy.

4 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: analysed sample is studied simultaneously by two methods: the first one is a indirect immunofluorescence (IIMF) reaction, while the second one involves a polymerase chain reaction (PCR); the IIMF provides using monoclonal antibodies "BCKK" (P-384D and 434D). The antibodies interact with the capsular antigen F1 specific for Y.pestis species, or the plasmid-temperature-independent surface protein PFV which is found in all Y.pestis strains and rare R-form Y.pseudotuberculosis strains. The bacteria luminescence shows the presence of native or fraction-less Y.pestis bacteria, or typical and PFV-atypical Y.pseudotuberculosis strains in the sample. The PCR is conducted by two pairs of primers vlm33for/ISrevl754 - specific for Y.pestis species, and JS - specific for Y. pseudotuberculosis species. The values derived with the first pair of the primers are estimated as positive if observing the amplicons in 400 bps, and with the second pair if observing the amplicons in 223 bps; the analysed sample is identified by the matching the IIMF and PCR values with the reference strains.

EFFECT: method provides quick identification and high-reliability differentiation of all strains.

7 tbl, 6 ex

FIELD: medicine.

SUBSTANCE: donor strain producing an isotope-marked bacteriophage which is pre-recovered from lysogenic strain V.cholerae eltor in the 1 concentration n·108 PFU/ml is produced and added with 5 mcc/ml of isotope 3H-thymidine, and kept for one day in a thermostat at 37°C to produce marked cholera phage. The latter is introduced in the amount 1 ml in a test tube with an indicator recipient strain in the ratio 1:1 for lysogenisation, incubated at 37°C within one day; 0.1 ml of the derived mixture is seeded on an agar plate, grown at 37°C within 20-24 hours, and the donor strain is recovered. The residual portion of the mixture is used to record radioactivity of the bacteriophage and recipient strain. The positive result is shown by radioactivity of the marked bacteriophages within the range 475-1269 p/min, and of the recipient strains within the range 78-103 p/min.

EFFECT: invention allows higher efficiency of detection and recording of the cholera phage in a 3H-thymidine marked cholera germ cell.

3 tbl, 4 ex

FIELD: medicine, ophthalmology.

SUBSTANCE: in lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula: At PC value being below 10.0 one should predict favorable disease flow, and at PC value being above 10.0 - unfavorable flow.

EFFECT: higher accuracy of prediction.

2 ex

FIELD: medicine, medicinal microbiology.

SUBSTANCE: method involves growing microorganism culture to be studied in solid nutrient medium followed by preparing microbial suspension and its incubation in the presence of lactoferrin. Control sample is prepared in parallel series. Control and experimental samples are incubated, supernatant is removed from bacterial cells and lactoferrin concentration is determined in supernatant of experimental and control sample by immunoenzyme analysis. Then anti-lactoferrin activity is calculated by difference of concentrations of residual lactoferrin in experimental and control samples. This method provides enhancing the sensitivity and precision in carrying out the quantitative evaluation of anti-lactoferrin activity in broad spectrum of microorganisms that is urgent in diagnosis and prognosis of diseases with bacterial etiology. Invention can be used in determination of persistent indices of microorganisms for assay of their etiological significance in pathological processes.

EFFECT: improved assay method.

3 tbl, 3 ex

FIELD: medicine, biology.

SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.

EFFECT: improved an valuable properties of nutrient medium.

3 ex

FIELD: medicine, cardiology.

SUBSTANCE: in peripheral blood one should detect the level of CD95(+) and CD16(+) neutrophilic granulocytes and at combination of increased level of CD95(+) neutrophilic granulocytes by 4 times and more and CD16(+) neutrophilic granulocytes by 0.6 times against the norm with ECG signs of myocardial infarction one should predict lethal result of large-focal myocardial infarction.

EFFECT: higher accuracy of prediction.

FIELD: medicine, parasitology.

SUBSTANCE: one should carry out immunoenzymatic assay to detect diagnostic optic density and that of labeled immune complex in a plot's hole with tested serum measured in conventional units at wave length being 492 nm. One should calculate coefficient of antibodies concentration measured in conventional units by the following formula: CAC = (Odtsh - Odd) x 100, where CAC - coefficient of antibodies concentration, Odtsh - optic density of the hole with tested serum, Odd - diagnostic value of optic density, 100 - coefficient of serumal dilution. By CAC value one should detect the titer of antibodies to Lamblia intestinalis antigens to interpret results of the trial. The method enables to study the dynamics of disease flow.

EFFECT: higher efficiency and accuracy of diagnostics.

1 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: the present innovation deals with studying and treating diseases of inflammatory, autoimmune and degenerative genesis. One should perform sampling of heparinized blood followed by its sedimentation to obtain blood plasma with leukocytes and centrifuging to isolate the latter which are washed against erythrocytic and serumal admixtures, and, also, it deals with calculating the number of cells in samples out of leukocytic suspension after incubation (B) for 1.5 h at 37 C in holes of plastic microplotting board, out of leukocytic suspension one should additionally prepare two samples, one should be applied to calculate total number of leukocytes before incubation (A), the second sample undergoes incubation at the same mode at addition of autoserum to calculate the number of cells remained after incubation (C). One should state upon adhesive properties of leukocytes by the index of spontaneous adhesion (D), where D=(A-B)/B.100%, and effect for enhanced cellular adhesion under the impact of autoserum should be detected by the value of K=(B-C)/C.100% at K ≥ 30%, where B - C - the number of cells undergone additional adhesion after addition of autoserum. The present innovation widens functional possibilities of the suggested method due to obtaining additional values depicting adhesive properties of blood leukocytes.

EFFECT: higher accuracy of detection.

FIELD: medicine, immunology.

SUBSTANCE: one should carry out reaction of blast-transformation, detect proliferation of T-lymphocytes activated with antibodies to CD3 in the presence of interleukin-7 (ACT IL-7) and in the presence of interleukin-7 and dexametazone (ACT IL-7 D), calculate the index for dexametazone action as the ratio of ACT IL-7 to ACT IL-7 D, moreover, the value of dexametazone action index being above 1.2 indicates increased production of cytokins that suppress T-lymphocytes in neonatals. The method enables to detect functional defect of immune system that characterizes neonatal period.

EFFECT: higher efficiency of detection.

2 ex

FIELD: medicine.

SUBSTANCE: method involves measuring forced exhalation volume per 1 s (FEV1) in l, full right ventricle evacuation time (RVE) in ms and angiotensin II value (AII) in ng/l. Discriminant relationship is built as D=0.504·RVE+3.038·FEV1 - 2.0·AII. D being less than 83.88, pulmonary hypertension occurrence is predicted within 1 year. D being equal to or greater than 83.88, no pulmonary hypertension is predicted to occur.

EFFECT: enhanced accuracy of prediction.

FIELD: medicine, medicinal immunology.

SUBSTANCE: method involves determination of heterophilic antibodies in human serum blood by the Paul-Bunnel's method relatively the level of circulating immune complexes, complement-activating properties of heterophilic antibodies by incubation of standardized ram erythrocytes with 0.8% serum for 30 ± 5 min and the following measurement of the erythrocytes lysis degree. The measurement of the effector function coefficient of heterophilic antibodies is carried out by the complement system Keff.f.h.a.-c.s. by the formula: Keff.f.h.a.-c.s. = Y/Tg.a. wherein Y means a lysis degree, %; Tg.a. means a reverse titer of heterophilic antibodies to ram erythrocytes. The damage assay is carried out by comparison of the immune status with the relative level of circulating immune complexes in serum. Method provides detection of preclinic from of immunodeficiency and autoimmune diseases that opens the possibility for their prophylaxis at most early stages of development. Invention can be used for assay of damage in the immune status in human serum blood.

EFFECT: improved method for assay.

5 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: method involves concurrently examining anti-inflammatory IL-4 level in blood serum and lacrimal fluid. The value being within the limits of 60-70 pg/l in blood serum and 5-15 pg/l in lacrimal fluid, disease prognosis is considered to be unfavorable. The IL-4 concentration being within the limits of 90-100 pg/l in blood serum and 20-30 pg/l in lacrimal fluid, disease prognosis is considered to be favorable.

EFFECT: high accuracy of diagnosis.