Porous composite chitosan-gelatin matrix for bone defect filling

FIELD: medicine.

SUBSTANCE: invention refers to medicine and concerns composites for waxed reconstruction of the injured bone tissues. High-porous elastic chitosan-gelatin matrixes of porosity 90 % or more consist of chitosan and contain gelatin up to 60 wt % and sodium lauryl sulphate up to 0.2-0.4 wt %. The porous structure is produced by introducing the additives, freezing and sublimation drying.

EFFECT: due to elasticity and high porosity, said sponges fill bone defects with a minimum clearance that promotes the uniform formation of bone tissue throughout the defect; high solubility of matrixes provides fast bone tissue genesis.

1 tbl, 1 ex

 

The invention relates to medicine, namely plastic reconstruction of damaged bone tissue.

The most promising for rapid recovery of bone tissues are porous composite ceramic materials. High porosity contributes to a more rapid resorption of the material and consequently a more rapid recovery of bone tissue. A well-developed pore structure also contributes to the formation of new bone tissues throughout the volume in the pores and channels of porous materials. To increase beactively and resorbable possible through the use of porous composites containing chitosan (chitosan matrices). This polymer has bioactive and anti-viral properties. The advantages of porous composite materials based on chitosan compared to porous ceramics can be attributed to their high flexibility and the possibility of obtaining them without using high-temperature firing. Due to the elasticity and porosity of chitosan matrices will easily fill bone defects with minimal gap between the bone and the implant.

The closest technical solution and the achieved effect are the matrices on the basis of chitosan (CH), (Dominique J. GriVon, M. Reza Sedighi, David V. SchaeVer,b, Jo Ann Eurell, Ann L. Johnson, Chitosan scaffolds: Interconnective pore size and cartilage engineering // Acta Biomateriala 2 (2006) 313-320). The matrices were obtained by dissolving chitosane in acetic acid solution. Then from the solution was removed excess water method of drying and freezing. In the process of drying is the removal of the frozen solvent by sublimation. When freezing of the initial solution in the form of particles of ice crystallizes aqueous solution, which is then removed by drying. As a result, the remote location of the ice formed rounded pore sizes up to 100 microns. The disadvantage of the resulting matrices is their low solubility, which prevents the rapid recovery of bone tissue, and low porosity. Low porosity (about 80%) sponges complicates the possibility of deformation of the jaws when filling bone defects and as a consequence leads to a tight fit of the sponge to the bone, which can lead to the formation of voids in the formation of bone tissue.

The technical result of the invention is the obtaining of porous chitosan-gelatin matrices (HIM), with a higher solubility and porosity of more than 90%, with the amount rounded pores of 20 to 400 microns in diameter and elongated pores from 20 to 3000 μm in length and from 10 to 200 microns in diameter.

The technical result is achieved in that the porous composite chitosan-gelatin scaffold for filling bone defects according to the invention contains gelatin in to the number to 60 wt.% and sodium lauryl sulfate, 0.2-0.4 wt.% and has a porosity of more than 90% dimensions rounded pore diameter of 20 to 400 μm and elongated pores from 20 to 3000 μm in length and from 10 to 200 microns in diameter.

The composition HGM not known. To obtain HIM in a solution of ethanoic acid are dissolved chitosan and gelatin at a pH of from 4 to 5. After complete dissolution the solution is added to the foaming agent - additive ammonium carbonate and sodium lauryl sulfate.

When interacting with ethanoic acid ammonium carbonate is bubbling carbon dioxide with formation of pores rounded shapes.

The obtained foamed solution is frozen and subjected to freeze-drying. In the process of freezing and freeze drying the formed final uniform structure matrix, characterized by high porosity of more than 90% with the size of the rounded then up to 400 microns in diameter channel and then to 3000 microns in length and up to 200 microns in diameter. Then, the matrices were washed and dried at a temperature of 80°C, while the remaining ammonium carbonate is completely removed. The result is a porous composite matrices with different composition and porosity with different size depending on the concentration in solution of initial components.

When the content is more than 60 wt.% gelatin during aging in water sponges are rapidly dissolved with partial or complete destruction. When the content of sodium lauryl less than 0.2 wt.% the structure obtained with non-uniform pore distribution, the highest content is not effective, because the structure is practically unchanged and reduces the elasticity and strength of the matrices.

Example. 1 Powder chitosan 1 g of gelatin 1 g dissolved in 20 ml of a solution of ethanoic acid at pH=4-4,5. Then in the solution under stirring was added 1 g of ammonium carbonate and sodium lauryl 0,008, After freezing at temperatures below minus 18°C foam mass was placed in a freeze dryer. After freeze-drying, rinsing, and drying in air at 80°C has received plastic composite matrix with a porosity of 95-98% and pore size rounded - 20-300 microns in diameter. Solubility in 7 days was 11-13 wt.%.

Solubility was determined by the weight loss during curing HIM in the water. Samples weighing 0.5 g were kept in 50 ml of distilled water at 37°C for 7 days, after which they were removed, dried at 60-80°C until dry, when the mass of the samples was the same. Calculation of solubility was carried out according to the formula:

P=(m1-m2)/m1×100%,

where m1- weight HIM to test solubility;

m2- weight HIM after the test for solubility.

In accordance with the examples were also produced samples HIM having compositions within the stated and defined their properties in comparison with the prototype. The results obtained are summarized in table.

Porous composite chitosan-gelatin scaffold for bone filling the Def is tov, characterized in that it contains gelatin in an amount up to 60 wt.% and sodium lauryl sulfate, 0.2-0.4 wt.% and has a porosity of more than 90% dimensions rounded pore diameter of 20 to 400 μm and elongated pores from 20 to 3000 μm in length and from 10 to 200 microns in diameter.



 

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1 tbl, 9 ex

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4 tbl

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6 cl, 3 ex

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