Device to sample gas medium for sorbent and method to prepare it for operation in compartment of manned spacecraft
FIELD: space engineering.
SUBSTANCE: device to sample a gas medium for a sorbent in a compartment of a manned spacecraft includes a bellows aspirator with a working volume of around 100 cm3 and a sampler with an absorber, arranged in the form of a sorbent-filled tube body made of stainless steel with the length of around 150 mm and inner diametre of not more than 6 mm, at the ends of which the thread is cut, onto which captive nuts are screwed - as plugs of duraluminium with a Teflon seal, joined with the absorber body by a flexible synthetic thread, and the tube ends are closed with a glass wool with Teflon limiters. Also there is a method provided to sample a gas medium for a sorbent in a compartment of a manned spacecraft by pumping of an examined air in the manned spacecraft through a previously prepared device. Besides, preparation includes thorough degreasing and washing of the inner surface of the tube with subsequent drying in the nitrogen atmosphere at 300°C prior to the sorbent charging into the absorber; removal of substances absorbed by a used sorbent by means of nitrogen blowdown via the sorbent and the sampler sealing.
EFFECT: accuracy and reliability of gas sampling for sorbents when used in conditions of a manned space flight.
4 cl, 1 dwg
The present invention relates to the selection of sorbents sample gas environment in conditions of weightlessness in the compartments of manned spacecraft (PKA) and is based on pumping a metered sample of the investigated gas medium through probsably with the sorbent, which captured the trace amounts of organic substances. To identify these substances and their quantitative determination in the laboratory of absorbers using a stream of inert gas and thermal effects of these trace amounts extracted from the sorbent in the form determined by the volume of the gas sample.
The prior art various designs samplers, however, for solving the problem of space research and experiments they are unsuitable due to the impossibility of combining with equipment spacecraft or research experimental module.
Known gas sampler samples SU 1608461, G01N 1/22 for geological purposes. Its design and method of its use cannot be applied due to the nature of design for space research in hermometer.
A device for sampling gases containing gas pipeline, probe, conical chamber, a sampling tube and the analyzer (SU 180411, G01N 1/22, 1966).
The advantages of this device include the ability to use as is analizator analytical filter, sedimentation column and photoelectric counter.
The main disadvantage of this device is that it is not isokinetically gas sampling, since there is no equality between the average velocity of flow in the selected tube in the gas line. In addition, at low speeds the sample gas is the deposition of impurities in the selected tube. All this greatly reduces the reliability of the analysis results and representativeness of the sample.
The known device for sampling gases of high pressure, which can be used for analysis and control of contents mechanical verdadeira particles (impurities) in compressed gases (air, nitrogen, helium, hydrogen, argon, neon, xenon, oxygen, and other gases)used in the rocket-space technology, aviation, automotive and other for Example, a device (EN 2152017, G01N 1/22, 27.06.2000) contains a probe that is connected with him of the sampling tube with a shut-off valve, throttle, conical chamber and sampling pipe and the analyzer samples. The orifice is made in the form of a combined nozzle consisting of series-connected input confused, cylindrical and output cone parts. The conical chamber is integral with the upper part that extends into the diffuser portion of the combined nozzle and the bottom, is covered at the base. The device provides the reliability and accuracy of analysis of mechanical verdadeira particles (impurities) in compressed gases.
The disadvantage of this device is its narrow functionality, which allows to obtain a sample of gas at high pressures and velocities of gas flow. Known, or other similar device for sampling gases containing probe, sampling tube, for example SU 819613, G01N 1/22, 09.04.81, GOST 3022-70, p.4. However, all known devices have a major drawback - they are functionally designed for other studies. In addition, they are characterized by the complexity of operative adjustment costs by maneuvering valves on the bypass and analysisfor lines (opening, closing). In addition to complicating conditions, this fact does not provide the necessary reliability of the device.
A device for sampling the compressed air to a filter device containing a gas pipeline probe shut-off valve, pressure gauge, thermometer, rotameter, regulating valve, filter element and the throttle on the exit fitting (Baibakov FB and other Control impurities in compressed gases, M.: Chemistry, 1989, p.140, RIS).
The advantage of this device is its structural simplicity, but its main drawback is the possibility of changes in the process of sampling not only concentration is AI, but disperse composition, as well as larger particles, as shown by tests, are deposited on the walls of the sample tube, and submicron particles slip through the security filter. Install the throttle on the exit fitting violates the condition isokinetic sampling and reduces the reliability of the results.
The objective of the invention is the provision of representativeness, accuracy and reliability of the sampling gas sorbents for use in the manned space flight.
The selection of sorbents sample gas environment based on pumping a metered sample of the investigated gas medium through probsably with the sorbent, which captured the trace amounts of organic substances. To identify these substances and their quantitative determination in the laboratory of absorbers using a stream of inert gas and thermal effects of these trace amounts extracted from the sorbent in the form determined by the volume of the gas sample.
Thus, taking into account Skopelitis tasks, the technical result - the development of the sampling capacitance and method of sampling for research in weightlessness is achieved by a device for sampling gas environment on the sorbent in the compartment manned spacecraft includes bellows suction pump with a working volume is about 100 m cm 3and probsably absorbent, made in the form of filled with sorbent housing-stainless steel tube with a length of about 150 mm and an inner diameter of not more than 6 mm, the ends of which are threaded, which are screwed nut-caps made of aluminium with Teflon seal connected to the housing of the flexible absorber synthetic thread, and the ends of the tube are covered glass wool with Teflon stoppers.
The task is solved in that the device comprises a polymeric sorbent of Tenax, sorbent Silica gel D-08, or any other sorbent, depending on the analyzed substances.
The task is also solved using the method of sampling of gas environment on the sorbent in the compartments manned spacecraft using the device described above, by pumping the investigated air in manned spacecraft through pre-prepared device, and the training includes a thorough degreasing and washing the inner surface of the tube, followed by drying in nitrogen atmosphere at 300°C before loading of the sorbent in the absorber, removing sorbed on the sorbent substances by blowing through the sorbent nitrogen speeds of 30-60 ml/min for 6-8 hours at a temperature of 250°-300°, and the flow is subjected to at least 0.5 l of the investigated air when the duration is eljnosti one selection about 3 minutes.
The authors of the present invention is not known similar technical solutions, and therefore the claimed set of attributes corresponds to conditions of patentability.
The invention is illustrated in the drawing, which shows a device for the selection of the sorbent sample gas medium in the compartments manned spacecraft.
The drawing shows a device for the selection of the sorbent sample gas medium in the compartments manned spacecraft, where
1 - bellows hood,
2 - probsably absorbent,
The invention is carried out as follows.
Equipment and materials:
Wardrobe-thermostat with gas line, a rotameter and the on / off valve,
The compressed nitrogen GOST 10157-79,
Sorbent "Tenax TA"
Sorption tube "Gerstel",
Covers for aspirators and sinks.
For sampling gas medium in the compartments PKA used probsably and manually operated bellows suction pump with a displacement of 100 cm3.
The sink is a stainless steel tube with a length of 150 mm and an inner diameter of 6 mm At the ends of the tubes are threaded, which are screwed nut - caps made of aluminium with a Teflon seal. The tube is filled with sorbent. In order sorbent navyseals and to maintain the density of the packing, the ends of the tube are closed with glass wool with Teflon stoppers. The stub and the body of the absorber are connected by a flexible synthetic thread.
The loaded sorbent sinks preceded by a thorough degreasing and rinsing the inner surface of the tubes, followed by drying in nitrogen atmosphere at 300°C. Before being sent to the manned spacecraft (PKA) absorbers are trained, consisting in the removal of sorbed on the sorbent substances by blowing through the sorbent nitrogen speeds of 30-60 ml/min for 6-8 hours at a temperature of 250°-300°C. Probability undergone heat treatment within the above specified time, removed from the Cabinet-thermostat, seal and send to the object.
Characteristics of probabilitv:
The error of the measured air samples - 5%.
The number of pumped air through a single absorber when sampling for organic trace amounts - 500 cm3.
The number of pumped air through a single absorber when sampling on freon 218-100 cm3.
The degree of concentration of organic trace contaminants -25 times.
The degree of concentration of freon 218 5 times.
Remove the aspirator from the case; remove probsably; remove the nut caps with probabiliy; in with the accordance with the marking on probabilite to tightly connect probsably with the aspirator; dramatically compress the bellows aspirator and release (the open end of promotability must be in the desired sampling point air); to wait for the full straightening of the bellows, an aspirator, an operation to compress and flatten bellows aspirator repeat 4 more times (total 5 times); disconnect probsably from the suction pump and to produce sealing its ends by nuts, plugs; to write in your journal: room promotability, sampling location, date of sampling, it is desirable to clarify microclimatic conditions in the compartment PKA during selection probsably to remove the styling, the suction device to remove the case and prepare probsably to return.
Sample preparation for gas chromatographic analysis.
To install probsably in wardrobe - thermostat; to raise the temperature in the Cabinet - thermostat to 200°C; decarbonate organic traces of contaminants from the sorbent probabilita stream of nitrogen or helium with a speed of 30-60 ml/min; the desorbed sample is transferred to the sorption turbo "Gerstel" for subsequent analysis on a gas chromatograph with mass spectrometric detection, to perform the decoding results of the analysis taking into account the degree of concentration on the sorbent.
The invention is intended for the qualitative identification and quantitative determination of trace volatile organic compounds (alcohol is, ketones, esters, aliphatic and aromatic hydrocarbons in air, oxygen, nitrogen and helium. Separation of the mixture components is conducted in a conventional gas chromatography on capillary columns coated with a stationary liquid phase. Assessment of the components is carried out using a mass-selective detector (MSD).
The invention is based on extracting probabilism organic trace contaminants from pumped through the sorbent studied air, the subsequent extraction of trace and transfer on the sorption tube "Gerstel with subsequent recovery system Gerstel thermal desorption for HP 6890 (TD) and injector Gerstel CIS 4 cryogenic cooling for HP 6890 for automatic injection (DRI) concentrated organic substances in the capillary column gas chromatograph HP 6890 (GC), which is part of the system 6890/5973N (GC/MSD). Substances emerging from the capillary column in a specific sequence, proceed in the mass-selective detector (MSD), which is conducted qualitative and quantitative identification of trace contaminants. For accurate identification of trace contaminants in the samples library is used NIST-98 (BMS), containing in the computer's memory 130,000 mass spectra and including structural formulas 275000 compounds. Calibration / mass spectrometer for subsequent number of the quality assessment of target component concentration when performing analyses of samples is carried out using certified sources mikropotokami in a dynamic setting "Microgap". Method / mass spectrometric determination of the concentration of pollutants in the atmospheric air approved by the Russian State sanitary and epidemiological regulation of the Russian Federation in the "Methodological guidelines on / mass spectrometric determination of volatile organic compounds in ambient air". The results of these analyses are used by the Russian toxicologists to assess the quality of the air environment of manned space germopulse, including orbital space stations.
The claimed device enables the sampling of air on Board the orbital station for further analysis of trace contaminants in the laboratory using GC/MSD method. So when pumping has not come leakage through the sorbent concentration, the volume of the pumped air is limited to 500 cm3. The duration of one selection is 3 minutes.
For promotability AK-IM the selected sorbent of Tenax, which is particularly effective for the concentration of toxic contaminants from the air. This polymer sorbent (poly-2,6-diphenyl-p-phenyleneoxy) has a low polarity, non-specific adsorption surface and has a very high thermal stability. The polymer based on poly-2,6-dispel-p-phenyleneoxy can be applied at 320°C, and the term is gravimetricheskie studies have shown, that at 400°C is not observed appreciable loss in mass of Tenexa, and only at 450°C weight begins to decrease. Tenax does not react with most organic substances even at temperatures above 300°C, and the efficiency of adsorption and desorption of trace organic compound is more than 90%. The advantages of this polymer include the possibility of high-speed sampling, the sample can be selected for 10-20 seconds) and high adsorption capacity for compounds with different molecular weight, and efficiency of sorption of trace contaminants on Tenace remains at the level of 90% and air velocity of up to 9 liters per minute. Higher adsorption characteristics of Tenax TA (AllTech catalog. 1997, p. 210).
Performed laboratory tests on the collection and analysis of samples containing organic compound classes of aldehydes, ketones, alcohols, aromatic and saturated hydrocarbons, unsaturated and halogenated hydrocarbons, esters order to determine the possibility of leakage of substances through probsably and determine modes of desorption for the most complete extraction with sorbent. For testing conditions for the extraction of trace contaminants from air Tanakam and complete extraction were selected substances, making the main contribution to the pollution of obite what's hermozamente areas with long service lives. Tests have shown that to prevent leakage of substances through Tenax when sampling through probsably need to pump 0.5 liters of the investigated air. It is found experimentally that for substances with low volume retention, such as freon, you need to use stronger adsorption properties of the sorbents. Therefore, for sampling the freon 218 are used probability AK-1M, filled with Silica gel D-08.
Tests conducted on the retention of trace contaminants in probabilitic AK-IM for a long time (6 months) period in a regular microclimatic conditions. The test results showed that sample integrity is within 91-97%, tightness of probabilitv saved.
Tests conducted on the impact on sample integrity and efficiency of the sorbent Tenax transport and climatic effects. The result is positive, the tightness of probabilitv saved.
1. Device for sampling gas environment on the sorbent in the compartment manned spacecraft, including bellows suction pump with a displacement of about 100 cm3and probsably absorbent, made in the form of filled with sorbent housing-stainless steel tube with a length of about 150 mm and an inner diameter of not more than 6 mm, the ends of which Nara is Ana thread, which are screwed nut-caps made of aluminium with Teflon seal connected to the housing of the flexible absorber synthetic thread, and the ends of the tube are covered glass wool with Teflon stoppers.
2. The device according to claim 1, characterized in that it comprises a polymeric sorbent of Tenax.
3. The device according to claim 1, characterized in that the sampling on freon 218 it contains a sorbent Silica gel D-08, or any other sorbent, depending on the analyzed substances.
4. Method of sampling of gas environment on the sorbent in the compartment manned spacecraft using a device according to any one of claims 1 to 3 by pumping the investigated air in manned spacecraft through pre-prepared device, and the training includes a thorough degreasing and washing the inner surface of the tube, followed by drying in nitrogen atmosphere at 300°C before loading of the sorbent in the absorber, removing sorbed on the sorbent substances by blowing through the sorbent nitrogen speeds of 30-60 ml/min for 6-8 hours at a temperature of 250°-300° and sealing promotability, and pumping is subjected to at least 0.5 l of the investigated air when the duration of one selection about 3 minutes
SUBSTANCE: invention relates to cosmetic industry, in particular, to composition for oral care, efficient for reduction of bad breath. Method to produce composition for oral care, with preliminary identification of substances efficient to reduce bad breath related to tobacco smoke. Method to eliminate bad breath related to tobacco smoke including introduction of composition for oral care, containing cardamom oil or cranberry extract. Agent efficient to reduce concentration of pyridine or pyrazine compound available in an individual oral cavity as a result of tobacco goods smoking, and elimination of bad breath at the same time, representing cardamom oil or cranberry extract.
EFFECT: composition efficiently reduces bad breath related to tobacco smoke.
23 cl, 14 dwg, 15 tbl, 7 ex
SUBSTANCE: sample container for taking samples of human expired air on molecular markers in form of a 5-7 litre sack made from fluoroplastic F-26 with an aluminium socket piece on which there is a rubber tube which is hermetically closed by a steel plug with a cap.
EFFECT: invention provides a sample container suitable for analyses in weightlessness conditions.
3 cl, 2 dwg, 1 tbl
SUBSTANCE: invention refers to medicine and namely to medical diagnostics. There has been proposed express-diagnostic method of diabetes mellitus as per the content of acetone micro-impurities in the air breathed out by the patient, at that, evaluation of acetone content in the sample is performed with the instrument complex 'concentrating centrifuge - mass-spectrometre'.
EFFECT: possibility of observing the dynamics of acetone in real-time mode.
SUBSTANCE: invention refers to medicine, namely to medical diagnostics. There is offered early diagnostic technique of kidney affection caused by therapy with nonsteroid antiinflammatory drugs. In the patients, microalbuminuria is registered by semiquantitative method. Concentration of β2-microglobulin is evaluated by immunoenzyme method. If microalbuminuria index corresponds to the range (30-300) mg/ml, and/or β2-microglobulin exceeds 0.3 mg/ml, incipient character of canalicular and glomerular kidney apparatus affection is considered to be observed. Said method makes it possible to observe changes in kidney tissue, and to individualise therapeutic practice on the basis of the obtained result.
EFFECT: ensured possibility to observe initial structural changes in kidney tissue and to individualise therapeutic practice on the basis of the obtained result.
2 ex, 2 tbl
SUBSTANCE: method involves examining expired air moisture condensate for determining sodium, potassium, calcium content and hydrogen index. Bronchopulmonary diseases remission stage is predicted depending on sodium, potassium, calcium concentration and hydrogen index, and pulmonary carcinoma diagnosis is excluded or confirmed.
EFFECT: high accuracy of prediction.
3 cl, 3 tbl
SUBSTANCE: one should detect the content of ammonia along with organic amines in the air of mouth cavity due to intake of urea of normal isotopic composition of 12C 1H4 14N2 16O and detecting patient's infectiousness degree due to comparing the results of trials, moreover, one should detect the content of ammonia with accompanied organic amines I the air of mouth cavity should be performed after the intake of urea, moreover detection of initial level should be carried out before the onset of urea hydrolysis within 0-1.0 min interval, and detection of ammonia's loading content - during the period of active urea hydrolysis within 1-9 min interval after the intake of urea, moreover, air sampling out of mouth cavity, air supply onto pick up unit for ammonia transformation into a signal, detection of ammonia content with accompanied organic amines in the air and, also, detection of patient's infectiousness should be performed either once or periodically, or continuously. For this purpose one should apply device that contains means for air sampling out of mouth cavity, pick up unit for ammonia transformation into a signal, measuring unit, treatment unit, and indication unit, moreover, measuring unit is designed as a unit for signal measuring with possibility to transform them into digital form, pick up unit for ammonia transformation into a signal is designed as electrochemical sensing device its input being connected with a tube with means for air sampling, this device is additionally supplemented with memory unit and enhancing unit its input being connected with the output of pick up unit for signal measuring, and output of enhancing unit is connected with the input of the unit for signal measuring with possibility to transform them into digital form, its output being connected with the input of memory unit its output being connected with the input of treatment unit, moreover, the output of the latter is connected with the input of indication unit.
EFFECT: higher accuracy of diagnostics.
11 cl, 3 dwg, 5 ex
SUBSTANCE: tissue sections are incubated simultaneously with mixed primary and secondary antibodies. Primarily, the primary antibodies are applied on the tissues, and in 15 seconds of the incubation process - the secondary antibodies without washing with a buffer. It is followed with combined incubation for 4 minutes. A substrate-chromogen solution for the incubation is prepared immediately before the application, the sections are washed with a buffer solution and distilled water heated to temperature 37°C.
EFFECT: method allows fast and precise tumour histogenesis, detection of tumour cells within resection boundaries.
6 dwg, 2 tbl, 6 ex
SUBSTANCE: proposed system comprises filter accommodated in tight sampling container communicated with accumulating container. It comprises also controlled vacuum source, tight sampling container, receive/transmit station made up of tight container with union arranged at container cylindrical section to feed water for rinsing sample transfer line. System incorporates filtration unit arranged between sampler and receiver/transmit station and made up of tight overflow container accommodating filtration element and filtrate accumulation container provided with filtration element, tightly communicated with aforesaid container. Note here that system control, indication and data processing unit incorporating electro pneumatic and electric control elements provided with programmable logic controller and digital signal processing program. Additionally, system incorporates device to receive process samples that required pre-filtration or sedimentation, deaeration, harmful gas evacuation, and rinsing water. Said device represents n air separator. Besides, it includes sample vacuum feed device to feed samples to measuring device that consists of serially connected switch to receive fed process samples or rinsing water, flow measuring cell connected to vacuum generation and feed system of receive/transmit station. Note also that said switch is located below flow flask level and made up of closed vessel provided with covers with unions to supply various samples, and electric sample availability pickup arranged at its bottom. Said cell is closed by protective film and located nearby reference sample arranged on driven carriage. Note that receive/transmit station of analytical complex is located below the level of aforesaid flow measuring cell and features volume exceeding total volumes of fed sample and rinsing water, and communicated with flow cell and return line. Additionally, system comprises device to automatically feed metered amounts of rinsing water made up of water coarse filter. Also, it incorporates electro pneumatic control devices to control primary samplers, sample preparation and delivery to analysis points, receive signals from electric pickups and interacts with devices incorporated with the system in compliance with cyclogram. Additionally, it includes electric control device comprising operator panel with indication and visualisation means to control sample feed, those for rinsing, returning products into process, as well as receiving and processing measurement results and data transfer.
EFFECT: stationary conditions for liquid sample analysis.
9 cl, 2 dwg
SUBSTANCE: invention relates to field of medicine, namely to obstetrics. In order to confirm role of infectious process in uteroplacental region in genesis of combination of threat of abortion and development of fetus growth retardation syndrome in future in pregnant women with symptoms of abortion threat leukocyte intoxication index is determined by formula: LII=segmentonuclear + stab + immature + myelocytes/monocytes + lymphocytes + eosinophils. In case if LII is higher than 1.8, infectious nature of said complication is detected.
EFFECT: method makes it possible to diagnose endogenic intoxication in case of abortion threat and establish infectious cause of fetus development retardation syndrome.
2 ex, 5 tbl
SUBSTANCE: group of inventions relates to a concentration-lowering sampling apparatus designed to collect a gas sample from a sample space, having temperature which is essentially higher than normal temperature, and a method of collecting samples form the said sample space. The sampling apparatus has a sampling pipe, having a first end through which the stream of the sample enters the said pipe, and a second end. The sampling apparatus has a diluting apparatus designed to receive the stream of the sample from the second end of the sampling pipe and for dilute the sample. The diluting apparatus has a tubular gas-permeable first jacket which forms a sample channel, in which the second end of the sampling pipe enters, and a tubular gas-permeable second jacket around the first jacket, which forms between itself and the first jacket a space for diluted gas fed through the first jacket into the sample channel. The second jacket is joined by its end lying on the side of the sampling pipe to the sampling pipe in a first joining position. The outer diametre of the second end of the sampling pipe is smaller than the inner diametre of the first jacket, and the second end of the sampling pipe protrudes into the first jacket by a second distance from the first joining position, while facilitating direction of the diluted gas with formation of an essentially laminar protective stream lying longitudinally relative the sample channel and the surrounding stream of the sample. The method involves collecting the stream of the sample into the sampling pipe, directing the stream of the sample from the said sampling pipe to the sample channel formed by the first gas-permeable jacket. The diluted gas is then fed into the space for diluted gas formed by the second jacket surrounding the first jacket and joined by its end to the sampling pipe, and then from that space through the first jacket into the sample channel. Further, heat is transferred from the sample space to the space for diluted gas in order to heat the diluted gas which is in contact with the sampling pipe. The heated diluted gas creates an essentially laminar protective stream surrounding the stream of the sample coming from the sampling pipe into the sample channel, and lying longitudinally relative the sample channel.
EFFECT: efficient prevention of loss of sample material.
10 cl, 4 dwg
SUBSTANCE: method of creating a virtual model of a biological object involves fixing the biological object using cementing material in a three-dimension figure to form a specimen and dividing it into multiple parallel layers in a given sequence, obtaining an image of each layer of the specimen in digital format, entering the obtained image of each layer into a digital image processing unit to obtain a three-dimensional virtual image of each layer, merging the obtained virtual images of the multiple layers into a sequence which is the reverse of the given sequence until a virtual model of the specimen is obtained and deleting the image of the cementing material to obtain a virtual model of the biological object. Before fixing, the biological object is instantly frozen at cryogenic temperatures. The biological object is fixed by freezing the cementing material and the specimen is divided into layers in closed space by successively cutting the selected plane of the specimen with a discrete step of not more than 50 mcm. The image of each layer in digital form is obtained from the surface of the specimen formed after each cutting step. The device for realising the method has a unit for mounting the biological object, a rotating mechanism with cutting elements in form of cutters designed to divide the specimen with the biological object into layers, and a cooling cabinet in which the unit for mounting the biological unit is mounted with possibility of linear displacement in the longitudinal direction and with possibility of discrete linear displacements in the cross direction. The device also has an apparatus for obtaining digital images of the specimen, a unit for processing digital images of the specimen, and a control unit connected to the rotation mechanism with cutting elements in form of cutters, a mechanism for linear displacement of the unit for mounting the biological object in the longitudinal direction, a mechanism for discrete linear displacement of the unit for mounting the biological object in the cross direction and apparatus for obtaining digital images of the specimen.
EFFECT: use of the invention increases reliability of three-dimensional reconstruction of a pathology-biological object by avoiding distortion of its structure and form.
21 cl, 7 dwg
SUBSTANCE: to evaluate the nanodispersed copper action on tumour growth in experiment, a suspension of copper nanoparticles in the concentration 1 mg/ml in physiologic saline is introduced in eight doses of 1.25 mg/kg each, at total dose of 10 mg/kg to male rats grouped in three suffering transplantable sarcoma 45. In the first group, the introduction is local, in a tumour, in the second group - intraperitoneal, while in the third reference group, physiologic saline is introduced in the amount 0.3 ml in a rat. The introduction is carried out on 9th, 10th, 12th, 13th, 19th, 20th, 22nd and 23rd post- transplantation days; the animals are killed on the 29 post- transplantation day. The nanodispersed copper action on tumour growth is described by the following criteria - tumour weight, tumour volume, efficiency index, tumour growth inhibition percentage, morphological changes in tumour tissue.
EFFECT: method allows evaluating the nanodispersed copper action on transplantable sarcoma-45 growth.
FIELD: machine building.
SUBSTANCE: procedure consists in immersion of tubular casing of fuel rod into water electrolytic medium containing particles of iron oxide and in covering it at least partially with layer of iron oxide. Also, particles of iron oxide are produced by anode oxidising iron containing working electrode.
EFFECT: increased corrosion resistance of treated rod and its increased service life.
7 cl, 1 dwg
FIELD: machine building.
SUBSTANCE: device consists of rotating precipitating portion. A precipitator consists of a stabilising chamber, precipitating chamber and of a channel for centrate drainage. The precipitating chambers are divided with partitions with orifices for liquid flow. Precipitation is performed from a moving layer of liquid, while drying of sediment is done through additional orifices in the partitions between the chambers. The precipitating chamber rotates on a faceplate. A flat removable precipitating plate is set on the bottom of this chamber. Bigger particles settle near the partition between the stabilising and precipitating chambers, while finer particles settle further from the sad partition downstream on the flat removable precipitating plate. A rotating pipe line supplies suspension into the precipitator. The device is also equipped with a stationary portion of centrate reception made in form of a jacket for reception of centrate. A convex bottom of the jacket is inclined.
EFFECT: sedimentation of both large and fine particles of suspension during analysis, easy unloading of sediment with minimal losses and with maintaining order of settled particles.
SUBSTANCE: invention refers to a method of analysing oligosaccharides being components of low molecular weight heparins and very low molecular weight heparins in blood plasma. A method of analysing β-unsaturated oligosaccharides in blood plasma. Application of the method for quantitative analysis of enoxaparin, octaparin, bemiparin or tinsaparin. Application of the method for quantitative analysis of octasaccharides (versions).
EFFECT: more adequate oligosaccharide analysis.
15 cl, 2 dwg, 2 tbl, 2 ex
SUBSTANCE: conjunctival cell sample is collected by pressing a bulbar conjunctiva of an examined eyeball at 2-3 mm above a limb in the meridian 12 hours with a soft contact lens (SCL) placed with its concave side on a tonometre. Then, the SCL is removed from the tonometre and turned out so that the collected sample is at the bottom of the SCL which is fixed in 95% alcohol by single dip. Then, the SCL is washed with distilled water, then coloured with hematoxylin for 10-15 seconds, wash with distilled water and air-dried. Thereafter, the SCL is placed on a slide surface for cytological analysis.
EFFECT: invention allows simplifying the method for conjunctival cell preparation for cytological analysis, reduced analysis time and cost.
FIELD: automatical aids for sampling liquids.
SUBSTANCE: system for sampling and delivering filtrate has filter submerged into tested medium and connected with collecting tank and vacuum pressure source which is connected with top hole of collecting tank by means of pneumatic pipe. System has sample receiving tank connected with collecting tank and control unit which has first output to be connected with vacuum pressure source. Collecting tank has two separated chambers - washing chamber and dispatching chamber. Lower hole of washing chamber has to be lower hole of collecting tank and side hole of dispatching chamber has to be side hole of collecting tank. Floating valve is installed inside washing chamber to shut off lower and top holes. Filter is connected with lower hole of collecting tank through sampling pipe. Side hole of collecting tank is connected with lower hole of tank for receiving samples through sampling pipe. Flow-type sensor and check valve are installed inside transportation pipe. Output of flow-type sensor is connected with input of control unit; second output of control unit is connected with control input of analyzer.
EFFECT: improved precision of measurement of sample ion composition; prolonged service life of filter.
1 cl, 1 dwg