Method of substance antioxidant activity test

FIELD: medicine.

SUBSTANCE: zeolite antioxidant activity test is enabled by introducing a substance being tested into bodies of experimental animals. Biological products of tissues and organs of the experimental animals and control sets are prepared. Metabolic process indicator substances are evaluated. The pulmonary tissue, blood plasma, erythrocytes and thrombocytes are analysed for the content of lipid peroxidation products and natural antioxidants which are scored and summed up. The zeolite antioxidant activity is tested relatively to the normal values of the content of lipid peroxidation products and natural antioxidants which are defined as an arithmetical mean of the relevant values received in the animals of a control set.

EFFECT: enabled reliable zeolite antioxidant activity test at the enabled comparative evaluation of substances by this parametre.

2 tbl, 1 ex

 

The invention relates to the field of medicine and pharmacology, and can be used for evaluation of antioxidant properties of natural mineral materials, as well as various medical preparations and food products.

A known system for testing antioxidative activity of polymer alkylarylsulfonates, as a target molecule of the oxidizing agents used salicylate. Hydroxyl radical reacts with salicylic acid (2-oksibenzoynoy acid) with the formation of two deoxybenzoin acids, in particular 2,3 - and 2,5-deoxybenzoin acid. These gidroksilirovanii products are proof of the formation of OH. Determination of 2,3 - and 2,5-deoxybenzoin acids perform using liquid chromatography high-resolution electrochemical detector. For education and discovery HE used suspension 10 μm FeCl3, 1.0 mm H2O2, 1.0 mm ascorbate, and 10.0 μm salicylic acid. Add 0.1 ml of normal saline or tyloxapol (final concentration from 0.0 to 10 mg/ml). The reaction mixture is maintained at 45°C for 30 min and centrifuged for 10 min at 1200 g. The supernatant centrifuged (Beckman Microfuge E) through the tubular filter (0.22 micron) (PGC Scientific N 352-118) at 15000 g 100 ál sample of the eluate served in the column GHUR 018 RP (250×4,7 mm Beckman N 235329). Gidrauxilirovanne the e products of salicylate determine quantitatively using an electrochemical detector (Coulochem (ESA model A), moreover, the reduction potential of the detector set equal to 0.40 B (DC). The oxidation potential of the safety cell (used as a screen) set to +0,40 B (DC). The measurements are performed twice (EN 2147235, 10.04.2000).

Also known another method designed for the analysis of peptides, consisting in determining the physical indicator of peptides with matching 3-hydroxypyridine. One of these physical characteristics are based on electrochemical detection. The method consists in injecting an aliquot of a biological fluid, such as urine, electrochemical detector, based on the redox potential. It is recommended to use amperometric or coulometric detector. Coulometric detector is more sensitive in this system, since it allows complete oxidation or restore the analyzed molecules in column eluent. Screening or guard electrodes can be placed in the ascending direction from the analytical electrode by selective oxidation or restoration of interfering substances, a significant improvement in the selectivity. For the destruction of interfering substances establish one or more cells pre-treatment (EN 2139541, 10.10.1999).

Known quantities of the config method for determining alpha-tocopherol, which is an antioxidant, in which the electrochemical determination of tocopherol by the method of cyclic voltammetry. This method is based on the ability of Tocopherols to a 1-electron oxidation and is implemented using the system for electrochemical measurements, consisting of a programmer PR-8, a potentiostat PI-50 and two-coordinate device registration PDA-1. Measurements conducted in the electrochemical cell coupled with a system for electrochemical measurements. The working volume of the cell 3 ml as a working electrode used stationary platinum disk electrode (ø 2 mm). The silver chloride reference electrode with waterproof diaphragm. The auxiliary electrode is a platinum spiral. Since the electrochemical measurements were carried out in nonaqueous media, provided the analog compensation of the ohmic losses using a potentiostat PI-50 (EN 2102747, 20.01.1998).

However, all of the above technical solution is only suitable for the analysis of individual substances.

There is also known a method of determining the antioxidant activity of substances, including the introduction of a measured substance in the organism of experimental animals, the slaughter of experimental and control animals, the preparation of biological products from the tissues and organs in both groups of animals and identify, and quantify substances-lights the ditch metabolic processes in the body called animals (U.S. Pat. U.S. No. 4615877), (U.S. Pat. U.S. No. 4615877). However, the known method is built on the identification of only one parameter, varying widely depending on a number of factors that affect it, which reduces the reliability evaluation of antioxidant activity of a substance, in addition, the claimed method does not allow to evaluate the antioxidant activity of zeolites.

Zeolites are non-stoichiometric compounds, their compositions vary within wide limits, forming a series of solid solutions. Known for more than 40 mineral species of natural zeolites.

Natural zeolites are molecular sieves, i.e. microporous bodies, able to selectively absorb substances, the molecular sizes smaller than the sizes of micropores (for penetration in the adsorption cavity of the adsorbate molecule must have a critical diameter less than the size of the input window). Crystallochemical features, the ability to cationic exchange loss and absorption of water and other molecules without destroying the structural frame and determine other properties of the zeolites, which serve as ion exchangers and catalysts.

Natural zeolite in the respiratory tract is not absorbed not absorbed into the bloodstream itself as crystal, and transit passes, interacting only at the level of selective ion exchange and selective sorption by contact with blood, simpati the definition vessels, giving or taking micrometrology, catalyzing biochemical reactions.

The task, which directed the claimed solution, which seeks to ensure reliable evaluation of antioxidant activity of substances, including zeolites.

The technical result obtained by the solution of the problem, which seeks to ensure the possibility of obtaining accurate evaluation of antioxidant activity of zeolites and other natural and synthetic materials, while providing opportunities for comparative assessment of substances in this parameter.

To solve this problem, a method for determining the antioxidant activity of a substance, including the introduction of a measured substance in the organism of experimental animals, the slaughter of experimental and control animals, the preparation of biological products from the tissues and organs in both groups of animals and identify, and quantify substances indicators of metabolic processes in the body called animals, characterized in that the introduction of a measured substance in the organism of experimental animals perform by inhalation, in both groups, determine the content of products of lipid peroxidation and natural antioxidants in the lung tissue, in blood plasma, erythrocytes and platelets, which translate into points, and summarize according to the expression:

PAA=L+CP+e+T,

where PAA is an indicator of antioxidant activity of a substance, score (0 to 4); L is an indicator reflecting the content of products of lipid peroxidation and natural antioxidants in the lung tissue, score; KP - index, reflecting the content of products of lipid peroxidation and natural antioxidants in plasma, score; e - indicator reflecting the content of products of lipid peroxidation and natural antioxidants in erythrocytes, point; T is an indicator reflecting the content of products of lipid peroxidation and natural antioxidants in platelets, points, in addition, a larger value of the total score corresponds to a greater antioxidant the activity of the substances, the evaluation of antioxidant activity of a substance to produce a relatively normal values of the content of products of lipid peroxidation and natural antioxidants (N), which is defined as the arithmetic average of the corresponding values obtained on animals in the control group, and the value L is equal to 1 point, if the total value of points corresponding to the contents of Ceruloplasmin (C, mg/100 g), malondialdehyde (MD, nmol/g), Gidroperekisi (SE, nmol/g), Diene conjugates (DC, nmol/g) and Vitamin E (BE, µg/g) in tissues of the lung, is from 3 to 6, and the appointmentchenyy, smaller or larger above limit, the value L is equal to 0 points, if the content of ceruloplasmin deviates from normal values by no more than ±10%, t=1, when it exceeds the value N+10%, C=2, if less than the value N-10%, C=0, in this case, if the content of malondialdehyde deviates from normal values by no more than ±10%, MD=1, if exceeds the value N+10%, MD=2, if less than the value of N is 10%, MD=0, if the content of the gidroperekisi deviates from normal values by no more than ±10%, SE=1, if exceeds the value N+10%, SE=2, if less than the value of N is 10%, SE=0, while, if the content of diene conjugates deviates from normal values by no more than ±10%, DC=1, if exceeds the value N+10%, DC=2, if less than the value of N is 10%, then DK=0, if the content of vitamin E deviates from normal values by no more than ±10%, EE=1 when it exceeds the value N+10%, EE=2, if less than the value of N is 10%, then BE=0, and the value of KP is equal to 1 point, if the total value of points corresponding to the contents of Ceruloplasmin (C, mg/100 g), malondialdehyde (MD, nmol/g), Gidroperekisi (SE, nmol/g), Diene conjugates (DC, nmol/g) and Vitamin E (BE, µg/g) in blood plasma, is from 3 to 6, and for values smaller or larger above limit, the value of the index KP is equal to 0 points, in this case, if the content of ceruloplasmin deviates from normal values by no more than ±10%, t=1, when it exceeds the value N+10%, C=2, if less than the value N-10%, C=0, in this case, if the content of malondialdehyde deviates from normal values by no more than ±10%, MD=1, if exceeds the value N+10%, MD=2, if less than the value of N is 10%, MD=0, this, if the content of the gidroperekisi deviates from normal values by no more than ±10%, SE=1, if exceeds the value N+10%, SE=2, if less than the value of N is 10%, SE=0, while, if the content of diene conjugates deviates from normal values by no more than ±10%, DC=1, if exceeds the value N+10%, DC=2, if less than the value of N is 10%, then DK=0, while if the content of vitamin E deviates from normal values by no more than ±10%, BE=1 when it exceeds the value N+10%, BE=2, if less than the value of N is 10%, then BE=0, and the value e is equal to 1 point, if the total value of points corresponding to the contents of malondialdehyde (MD, nmol/g), Diene conjugates (DC, nmol/g) and Vitamin E (BE, nmol/ml) in erythrocytes, is within 1 to 4, and for values smaller or larger above limit, the value of e is equal to 0 points, if the content of malondialdehyde deviates from normal values not Bo the it than ±10%, then MD=1 when it exceeds the value N+10%, MD=2, if less than the value of N is 10%, MD=0, while, if the content of diene conjugates deviates from normal values by no more than ±10%, DC=1, if exceeds the value N+10%, DC=2, if less than the value of N is 10%, then DK=0, if the content of vitamin E deviates from normal values by no more than ±10%, then BE=1 when it exceeds the value N+10%, EE=2, if less than the value of N is 10%, then BE=0, and the value T is equal to 1 point, if the total value of points corresponding to the contents of malondialdehyde (MD, nmol/g), Diene conjugates (DC, nmol/g) and Vitamin E (BE, nmol/ml) in platelets is in the range of from 1 to 4, and for values smaller or larger above limit, the value of the index T receive 0 points if the content of malondialdehyde deviates from normal values by no more than ±10%, MD=1, if exceeds the value N+10%, MD=2, if less than the value of N is 10%, MD=0, while, if the content of diene conjugates deviates from normal values by no more than ±10%, DC=1, if exceeds the value N+10%, DC=2, if less than the value of N is 10%, then DK=0, if the content of vitamin E deviates from normal values by no more than ±10%, BE=1 when it exceeds the value N+10%, EE=2, if less than the value of N is 10%, then BE=0, in addition, the od of substances in the body of the animal is performed through his respiratory system.

Comparison of the characteristics of the claimed solution with signs analogues and prototype demonstrates its compliance with the criterion of "novelty".

The characteristics of the characterizing portion of the claims, solves the following functional tasks.

Signs indicating that the introduction of the measured substance in the organism of experimental animals perform inhalation by"allow accurate dosing of the evaluated substances obtained experimental animals.

Signs indicating that in both groups of animals (control and experimental) "determine the content of products of lipid peroxidation and natural antioxidants in the lung tissue, in blood plasma, erythrocytes and platelets", allow to increase the reliability of the estimate, since the latter is performed in a quantitative form, based on the "work" of several body systems, taking into account the wide spectrum of natural antioxidants, reflecting the response of these systems to the impact of the test material, the value of which often depends not or Vice versa depends substantially on parallel controlled parameters.

The signs are that the content of products of lipid peroxidation and natural antioxidants in the lung tissue, in blood plasma, erythrocytes and platelets "translate into points, and summarize an expression for the PAA=L+CP+e+T,

where PAA is an indicator of antioxidant activity of a substance, score (0 to 4); L is an indicator reflecting the content of products of lipid peroxidation and natural antioxidants in the lung tissue, score; KP - index, reflecting the content of products of lipid peroxidation and natural antioxidants in plasma, score; e - indicator reflecting the content of products of lipid peroxidation and natural antioxidants in erythrocytes, point; T is an indicator reflecting the content of products of lipid peroxidation and natural antioxidants in platelets, score allow quantitative assessment of antioxidant activity of any substance and the to compare and rank them according to this index.

The sign of "a larger value of the total score corresponds to a greater antioxidant activity of a substance allows to compare substances on their antioxidant activity.

Signs "evaluation of antioxidant activity of a substance to produce a relatively normal values of the content of products of lipid peroxidation and natural antioxidants (N), which is defined as the arithmetic average of the corresponding values obtained on animals in the control group allow you to establish a base for the quantitative estimation of parameters containing the Oia products of lipid peroxidation and natural antioxidants in the investigated tissues of the animal and their respective evaluation points.

Signs "value L is equal to 1 point, if the total value of points corresponding to the contents of Ceruloplasmin (C, mg/100 g), malondialdehyde (MD, nmol/g), Gidroperekisi (SE, nmol/g), Diene conjugates (DC, nmol/g) and Vitamin E (BE, µg/g) in tissues of the lung, is from 3 to 6, and for values smaller or larger above limit, the value L is equal to 0 points uniquely, from the standpoint of the claimed method to assess scoring options antioxidant effects, based on studies of lung tissues.

Signs "if the content of ceruloplasmin deviates from normal values by no more than ±10%, t=1, when it exceeds the value N+10%, C=2, if less than the value N-10%, C=0, in this case, if the content of malondialdehyde deviates from normal values by no more than ±10%, MD=1, if exceeds the value N+10%, MD=2, if less than the value of N is 10%, MD=0, while if the content of the gidroperekisi deviates from normal values by no more than ±10%, SE=1, if exceeds the value N+10%, SE=2, if less than the value of N is 10%, SE=0, while, if the content of diene conjugates deviates from normal values by no more than ±10%, DC=1, if exceeds the value N+10%, DC=2, if less than the value of N is 10%, then DK=0, while if the content of vitamin E deviates from n is malinich values by no more than ±10%, then BE=1 when it exceeds the value N+10%, BE=2, if less than the value of N is 10%, EE=0" provide an evaluation of the contents of the products of lipid peroxidation and natural antioxidants in the lung tissues and their ball assessment within a parameter definition HP

Signs "value KP is equal to 1 point, if the total value of points corresponding to the contents of Ceruloplasmin (C, mg/100 g), malondialdehyde (MD, nmol/g), Gidroperekisi (SE, nmol/g), Diene conjugates (DC, nmol/g) and Vitamin E (BE, µg/g) in blood plasma, is from 3 to 6, and for values smaller or larger above limit, the value of KP is equal to 0 points uniquely, from the standpoint of the claimed method to assess scoring options antioxidant effects, based on studies of blood plasma.

Signs "if the content of ceruloplasmin deviates from normal values by no more than ±10%, t=1, when it exceeds the value N+10%, C=2, if less than the value N-10%, C=0, in this case, if the content of malondialdehyde deviates from normal values by no more than ±10%, MD=1, if exceeds the value N+10%, MD=2, if less than the value of N is 10%, MD=0, while if the content of the gidroperekisi deviates from normal values by no more than ±10%, SE=1, if exceeds the value N+10%, SE=2, if less than the values N-10%, then GP=0, while, if the content of diene conjugates deviates from normal values by no more than±10%, DC=1, if exceeds the value N+10%, DC=2, if less than the value of N is 10%, then DK=0, if the content of vitamin E deviates from normal values by no more than ±10%, EE=1 when it exceeds the value N+10%, EE=2, if less than the value N -10%, VE=0" provide an evaluation of the contents of the products of lipid peroxidation and natural antioxidants in blood plasma and their score in the definition of the parameter KP.

Signs "value e is equal to 1 point, if the total value of points corresponding to the contents of malondialdehyde (MD, nmol/g), Diene conjugates (DC, nmol/g) and Vitamin E (BE, nmol/ml) in erythrocytes, is in the range from 1 to 4, and for values smaller or larger above limit, the value of e is equal to 0 points uniquely, from the standpoint of the inventive method, to evaluate the scores of the parameters of the antioxidant effects based on studies of erythrocytes.

Signs "if the content of malondialdehyde deviates from normal values by no more than ±10%, MD=1, if exceeds the value N+10%, MD=2, if less than the value of N is 10%, MD=0, while, if the content of diene conjugates deviates from the normal znachenie more than ±10%, then DK=1 if greater than the value N+10%, DC=2, if less than the value of N is 10%, then DK=0, if the content of vitamin E deviates from normal values by no more than ±10%, EE=1 when it exceeds the value N+10%, EE=2, if less than the value of N is 10%, EE=0" provide an evaluation of the contents of the products of lipid peroxidation and natural antioxidants in red blood cells and their score in the definition of the parameter E.

Signs "value T is equal to 1 point, if the total value of points corresponding to the contents of malondialdehyde (MD, nmol/g), Diene conjugates (DC, nmol/g) and Vitamin E (BE, nmol/ml) in platelets is in the range of from 1 to 4, and for values smaller or larger above limit, the value of the index T is equal to 0 points uniquely, from the standpoint of the inventive method, to evaluate the scores of the parameters of the antioxidant effects based on studies of platelets.

Signs "if the content of malondialdehyde deviates from normal values by no more than ±10%, MD=1, if exceeds the value N+10%, MD=2, if less than the value of N is 10%, MD=0, while, if the content of diene conjugates deviates from normal values by no more than ±10%, DC=1, if exceeds the value N+10%, DC=2, if less than the value of N is 10%, then DK=0, this, e, is whether the content of vitamin E deviates from normal values by no more than ±10%, then VE=1 when it exceeds the value N+10%, EE=2, if less than the value of N is 10%, EE=0" provide an evaluation of the contents of the products of lipid peroxidation and natural antioxidants in the blood platelets and their score in the definition of the parameter T.

The signs of the input substances in the body of the animal is performed through his Airways" simplify the implementation of the method with position proportioning action.

An example of a specific implementation.

The studies were carried out on outbred rats. For experimental activation of lipid peroxidation animals were exposed to low-energy laser radiation. Low-energy laser irradiation was chosen as a factor in certain dosages functional inhibitory activity of the cells of the system of local immunity. For irradiation was applied pulsed infrared laser "Agnis-L01" with a wavelength of 850 nm, pulse energy of 3.7·10-7J., repetition rate 240-1400 Hz and modulation 8-69 Hz. Was irradiated chest in 6 zones (subaxillary, scapulary and subscapularis, left, and right) for 10 seconds 1 time per day for 15 days.

Part of the animals before irradiation were subjected to inhalation using zeolite (mordenite and zeolite) tuff of the two fields (Kulikovskii and Aginskoe) of the Amur region. eality were ground using an ultrasonic disintegrator Bandelin Sonopulse 3400. The crushing of the zeolites was about 1-5 μm. Inhalation enter into the lungs of zeolites was performed ultrasonic nebulizer URSA-0,25P (Russia). The number of micronized zeolite and duration of stay of the animal in the cage 1 is the rate of the animals receiving doses of zeolite from 100 to 1000 mg/kg (1 times a day for 40 min).

Animals were divided into 4 groups of 20 animals: Control - intact animals; Laser - animals, obluchavshegosya low-energy laser; Kulikovskii+laser and Aginskoe+laser - animals that inhalation was administered zeolites Kulikovskii and Varinskogo fields (respectively) under irradiation by low-energy laser.

For the evaluation of antioxidant properties of zeolite determine the levels of products of lipid peroxidation in plasma and lung tissue of rats. Using known biochemical methods, including: preparation of lung homogenates; isolation and washing of whole red blood cells for in vitro experiments; isolation and washing of platelets for experiments in vitro; extraction of lipids from blood plasma and lung homogenates; determination of ceruloplasmin in plasma, determination of malondialdehyde; determination of diene conjugates; determination of lipid hydroperoxides; the determination of vitamin E.

Th the manufacture of homogenates of the lungs.

After the slaughter of animals, dissected thoracic and abdominal cavity, lungs perfesional 0.15 M KCl solution containing 5 mm Tris-HCl buffer, pH 7,4. Lungs were crushed scissors, weighed and homogenized in the homogenizer of the downs with the same buffer at a ratio of 1:5 for 1 minutes Prepared homogenate was used to determine the content of peroxide oxidation of lipids (Pol) and natural antioxidants.

The separation and washing of whole red blood cells for in vitro experiments carried out according to the descriptions given in the book of Ashkenazi IA Erythrocytes and internal thromboelastometry. - L.: Nauka, 1977, 155 S.

The separation and washing of platelets for experiments in vitro carried out according to the descriptions given in the book "the Aggregation of platelets: study methods and mechanisms" / Samal A.B., Cherenkevich S.N., Khmara NF - Mn.: University, 1990. - 104 S.

Extraction of lipids from blood plasma and lung homogenates is carried out by the method of Blaye-Dyer (see Kats M. Technique of lipid. Isolation, analysis and identification of lipids. - M.: Mir, 1975. - 322 S.).

Determination of ceruloplasmin in plasma. The method is based on the oxidation of p-phenylenediamine with the participation of ceruloplasmin (see Kolb VG, Kamyshnikov B.C. Clinical biochemistry. Minsk, 1976. - 312 S.).

Determination of malondialdehyde. MD was determined in plasma CROs and and lung homogenates by color reaction with thiobarbituric acid (TBA) (see Borodin E.A. Stabilization and reactivation of cytochrome P-450 phosphatidylcholine during the peroxide oxidation of lipids / Eouropean, Ahiataku // Biological membranes. - 1987. No. 7. - S-728).

The definition of diene conjugates. 0.1 ml of the alcohol solution of lipids made in the cuvette of the spectrophotometer SF-16, which was a 2.9 ml of absolute alcohol. The optical density was measured at a wavelength of 233 nm with swing beam 10 mm For translation used the coefficient of molar extinction of 2.2·10-5M-5cm-5(see Steel E.A. Method for the determination of diene conjugation of unsaturated higher fatty acids // Modern methods in biochemistry. - M.: Medicine, 1977. - P.63-64). The amount of diene conjugate expressed in nolah on g tissue or ml of blood.

Determination of lipid hydroperoxides. The number of lipid hydroperoxides was determined on the basis of their ability to oxidize ions Fe2+with the subsequent reaction of Fe3+with ammonium thiocyanate (see Romanova L.A. Method for the determination of lipid hydroperoxides using thiocyanate ammonium / Gahramanova, Identally // Modern methods in biochemistry. - M.: Medicine, 1977. - P.64-66).

Determination of vitamin E. vitamin E was determined in lipid extracts from plasma and tissues by a colour reaction with dipyridylium and FeCl3(see Kisilevich RE Determination of vitamin E in serum / R. Kisilevich, Chisquare // Lab. case. - 1972. No. 8. - S-475) in the modification Masturbarse (see Starberg M.A. Antioxidant properties of the combined preparations of phospholipids with a derivative of malonic and thiobarbiturate acid: Diss. Kida. the honey. Sciences / Masserberg. - Blagoveshchensk, 1996. - 178 C.).

Data of biochemical studies summarized in tables 1 and 2.

Table 1
Content of products of lipid peroxidation and natural antioxidants in the lung tissue and plasma in experimental groups
GROUPCeruloplasmin, mg/100 gDiene conjugates, nmol/gMD, nmol/gThe gidroperekisi, nmol/gVitamin E, ug/g
lung tissue
Control39,02±2,54 p<0,001221,76±14,86 p<0,0018,84±1,22 p<0,00290,58±4,4 p<0,001205,44±7,38 p<0,01
Laser33,43±1,34 p<0,001 299,34±18,67 p<0,00113,54±1.1 p<0,001102,21±6,7 p<0,001200,35±8,51 p<0,001
Aginskoe+laser38,3±1,94 p<0,001237,24±12,29 p<0,001to 12.44±1,38 p<0,00192,0±3,96 p<0,001198,26±9,83 p<0,001
Kulikovskii+laser38,98±2,56 p<0,001259,58±15,25 p<0,001made 11.32±1.35 p<0,00292,14±5,93 p<0,001197,5±8,60 p<0,001
blood plasma
Control21,44±1,27 p<0,00151,82±5,15 p<0,001to 4.92±1,15 p<0,0220,18±1,21 p<0,00127,16±1,69 p<0,001
Laser19,12±0,89 p<0,00168,23±5.6 p<0,0015,3±1,32 p<0,00129,89±2,05 p<0,00126,34±1,82 p<0,001
Aginskoe+laser11,58±4,82 the< 0,135,0±7,35 p<0,0014,96±0,8 p<0,005are 24.88±0,87 p<0,00129,08±2,16 p<0,001
Kulikovskii+laser21,18±0,55 p<0,152,72±6,1 p<0,01of 4.44±0,54 p<0,00227,24±2,28 p<0,00121,94±1,28 p<0,001

Table 2
Biochemical indices of products of lipid peroxidation in erythrocytes and platelets
Indicator
Group
Diene conjugates (nmol/g)MD (nmol/g)Vitamin E (µg/g)
ErythrocytesPlateletsErythrocytesPlateletsErythrocytesPlatelets
Control56,6±3,361,6±3,5 6,1±0,52,0±0,15,1±1,0
Aginskoe43,4±2,354,0±1,44,1±0,25,1±0,24,1±0,15,6±0,1
Kulikovskii47,8±2,556,2±2,04,3±0,25,3±0,33,7±0,15,3±0,3

Next, using the table data is defined by the formula PAA=L+CP+e+T the value of PAA.

For Varinskogo field

A. the Definition of products of lipid peroxidation and natural antioxidants in the lung tissue (L).

1) Ceruloplasmin (mg/100 g Content of ceruloplasmin 35,66±2,41 mg/100 g, which corresponds to N±10% (39,02±2,54 mg/100 g), so C=1.

2) of Malonic dialdehyde (MD), nmol/g Content MD 8,88±0.98 nmol/g, which corresponds to N±10% (8,84±1,22), then MD=1.

3) Gidroperekisi, nmol/g Content of hydroperoxides 89,4±4.8 nmol/g, which corresponds to N±10%) (90,6±4.4 nmol/g), then SE=1.

4) of Diene conjugates (DC), nmol/g Content of diene conjugate 235,5±9.9 nmol/g, which corresponds to N±10%) (221,7±14,8 nmol/g), i.e. DK=1.

p> 5) Vitamin E, ág/g vitamin E 190,1±5,5 µg/g, which is greater than N±10% (205,4±7,4 mg/g), mean VE=0.

Thus, L=1, since C(1)+MD(1)+GP(1)+DK(1)+VE(0)=4

B. Definition of products of lipid peroxidation and natural antioxidants in plasma (CP).

1) Ceruloplasmin (mg/100 g Content of ceruloplasmin to 20.28±3.24 mg/100 g, which corresponds to N±10% (21,44±1,27 mg/100 g), so C=1.

2) of Malonic dialdehyde (MD), nmol/g Content MD 3,72±0,39 nmol/g, which is less than N±10% (4,92±0.45 nmol/g), then DR=0.

3) Gidroperekisi, nmol/g Content of hydroperoxides 24,58±to 4.52 nmol/g, which corresponds to N±10% (20,18±1,21 nmol/g), then SE=1.

4) of Diene conjugates (DC), nmol/g Content of diene conjugate 23,62±4.4 nmol/g, which is less than N±10% (51,82±5,15 nmol/g), then DK=0.

5) Vitamin E, ág/g vitamin E 28,42±2,10 µg/g, which corresponds to N±10% (27,16±1,69), then VE=1.

Thus, KP=1, C(1)+MD(0)+GP(1)+DK(0)+VE(1)=3

C. the Definition of products of lipid peroxidation and natural antioxidants in erythrocytes (e).

1) of Malonic dialdehyde (MD), nmol/g Contents of the MD to 4.1±0.2 nmol/g, which is less than N±10% (5,5±0,3 nmol/g), then DR=0.

2) of Diene conjugates (DC), nmol/g Content of diene conjugate to 43.4±2.3 nmol/g, which is less than N±10% (56,6±3.3 nmol/g), then DK=0.

3) Vitamin E, nmol/ml in the vitamin E of 4.1±0.1 µg/g, which is greater than N±10% (4,1±0,1 µg/g), mean VE=2.

Thus, e=1, since MD(0)+DK(0)+VE(2)=2

, The Definition of products of lipid peroxidation and natural antioxidants in platelets (T).

1) of Malonic dialdehyde (MD), nmol/g Content of the MD of 5.1±0.2 nmol/g, which is less than N±10% (6,1±0.5 nmol/g), then DR=0.

2) of Diene conjugates (DC), nmol/g Content of diene conjugate 54,0±1.4 nmol/g, which is less than N±10% (61,6±3,5), then DK=0.

3) Vitamin E, nmol/ml in the vitamin E of 5.6±0.1 µg/g, which corresponds to N±10% (5,1±1,0), then VE=1.

Thus, T=1, since MD(0)+DK(0)+VE(1)=1

TOTAL: the Value of PAA for Varinskogo field

PAA=L+CP+e+T=1+1+1+1=4

For the Kulikovo field

A. the Definition of products of lipid peroxidation and natural antioxidants in the lung tissue (L).

1) Ceruloplasmin (mg/100 g Content of ceruloplasmin 32,27±1.0 mg/100 g, which is less than N±10% (39,02±2,54 mg/100 g), then C=0.

2) of Malonic dialdehyde (MDA), nmol/g Content MD 10,56±0,92 nmol/g, which is greater than N±10% (8,84±1,22), then MD=2.

3) Gidroperekisi, nmol/g Content of hydroperoxides by 110.6±10.9 nmol/g, which is greater than N±10% (of 90.6±4.4 nmol/g)mean SE=2.

4) of Diene conjugates (DC), nmol/g Content of diene conjugate 261,6±13 nmol/g, which is greater than N±10% (221,7±14,8 nmol/g), then DK=2.

5) Vitamin E, ág/g vitamin E 189,4±4,2 mg/g, which is less than N±10% (205,4±7,4 mg/g), mean VE=0.

Therefore clicks the zoom, L=1, since C(0)+MD(2)+SE(2)+DK(2)+VE(0)=6

B. Definition of products of lipid peroxidation and natural antioxidants in plasma (CP).

1) Ceruloplasmin (mg/100 g Content of ceruloplasmin 19,25±1,43 mg/100 g, which corresponds to N±10% (21,44±1,27 mg/100 g), so C=1.

2) of Malonic dialdehyde (MDA), nmol/g MDA Content 7,15±0,63 nmol/g, which is greater than N±10% (4,92±0.45 nmol/g), then MD=2.

3) Gidroperekisi, nmol/g Content of hydroperoxides 29,47±1.92 nmol/g, which is greater than N±10% (20,18±1,21 nmol/g)mean SE=2.

4) of Diene conjugates (DC), nmol/g Content of diene conjugate 59,85±5,63 nmol/g, which is greater than N±10% (51,82±5,15 nmol/g), then DK=2.

5) Vitamin E, ág/g vitamin E to 24.33±1,88 µg/g, which is less than N±10% (27,16±1,69), then VE=0.

Thus, KP=0, C(1)+MD(2)+SE(2)+DK(2)+VE(0)=7

C. the Definition of products of lipid peroxidation and natural antioxidants in erythrocytes (e)

1) of Malonic dialdehyde (MDA), nmol/g MDA Content of 4.3±0.2 nmol/g, which is less than N±10% (5,5±0,3 nmol/g), then DR=0.

2) of Diene conjugates (DC), nmol/g Content of diene conjugate of 47.8±2.5 nmol/g, which is less than N±10% (56,6±3.3 nmol/g), then DK=0.

3) Vitamin E, nmol/ml vitamin E to 3.7±0.1 µg/g, which is less than N±10%) (4,1±0,1 µg/g), mean VE=0.

Thus, e=0, since MD(0)+DK(0)+VE(0)=0

, The Definition of products of lipid peroxidation and the natural Academy of Sciences is ioxidant in platelets (T).

1) of Malonic dialdehyde (MDA), nmol/g MDA Content of 5.3±0.3 nmol/g, which is less than N±10% (6,1±0.5 nmol/g), then DR=0.

2) of Diene conjugates (DC), nmol/g Content of diene conjugate 56,2±2.0 nmol/g, which is less than N±10% (61,6±3,5), then DK=0.

3) Vitamin E, nmol/ml vitamin E to 5.3±0.3 ág/g, which corresponds to N±10% (5,1±1,0), then VE=1.

Thus, T=1, since MD(0)+DK(0)+VE(1)=1

TOTAL: the Value of PAA for the Kulikovo field

PAA=L+CP+e+T=1+0+0+1=2

Low-energy laser radiation used in the dosage has prooxidant action. As follows from the data of tables 1, 2 in group Kulikovskii+laser, compared with a group of "Control" in the tissue of the lungs and in the blood plasma was found a significant increase in the content of diene conjugate and MD in lung homogenate, the increase in the content of hydroperoxides, the decrease in the concentration of ceruloplasmin and vitamin E. It says on functional decline in the activity of the antioxidant system during inhalation of zeolites in the Kulikovo field in combination with low-energy laser radiation. This may be due to slight damage to the lung tissue by the zeolite in the Kulikovo field and stimulation of inflammation by laser radiation. Results in the group Aginskoe+zeolite were as follows: found MD increase in lung homogenate and the decline of ceruloplasmin, the increase of hydroperoxides and MD in the blood plasma. On the other hand, there is a statistically significant decrease of diene conjugates and statistically significant increase in the concentration of vitamin E in the blood plasma.

Thus, laser radiation, inducing lipid peroxidation (LPO), zeolites Varinskogo fields manifest themselves as substances with antioxidant properties (PAA=4). Zeolites in the Kulikovo field, being the type of crystal lattice mainly mordenite (needle-like structure), on the contrary partially stimulate FLOOR, which leads to decrease in PAA (PAA=2), up to a level substantially less the same indicator for zeolites Varinskogo field.

Method for determination of antioxidant activity of zeolites, including their introduction into the organism of experimental animals, the slaughter of experimental and control animals, the preparation of biological products from the tissues and organs in both groups of animals, identification and quantification of substances indicators of metabolic processes in the body called animals, and animal impact, the vast functional activity of the cells of the system of local immunity of the lung, for this irradiated chest animals in subaxillary, scapulary and subscapularis areas, right and left, through them osnago infrared laser with a wavelength of 850 nm, the pulse energy of 3.7·10-7J., repetition rate 240-1400 Hz and modulation 8-69 Hz, while the estimated zeolite in the organism of experimental animals administered by inhalation, and in both groups define the content of products of lipid peroxidation and natural antioxidants in the lung tissue, in blood plasma, erythrocytes and platelets, which translate into points, and summarize in the words:
PAA=L+CP+e+T,
where PAA is an indicator of antioxidant activity of zeolite, score (0 to 4);
L - indicator reflecting the content of products of lipid peroxidation and natural antioxidants in the lung tissue, score;
KP - index, reflecting the content of products of lipid peroxidation and natural antioxidants in plasma, score;
E - the indicator reflecting the content of products of lipid peroxidation and natural antioxidants in erythrocytes, score;
T - indicator reflecting the content of products of lipid peroxidation and natural antioxidants in platelets, score,
the evaluation of antioxidant activity of zeolite produce relatively normal values of the content of products of lipid peroxidation and natural antioxidants (N), which is defined as the arithmetic average of the corresponding values obtained for W the animals of the control group, moreover, the value L is equal to 1 point, if the total value of points corresponding to the contents of ceruloplasmin (C, mg/100 g), malondialdehyde (MD, nmol/g), gidroperekisi (SE, nmol/g), diene conjugates (DC, nmol/g) and vitamin E (BE, µg/g) in tissues of the lung, is from 3 to 6, and for values smaller or larger above limit, the value L is equal to 0 points, if the content of ceruloplasmin deviates from normal values no more than 10%, then C is 1 if greater than the value of N at 10%, then C is equal to 2, if less than the value of N at 10%, C is 0, if the content of malondialdehyde deviates from normal values not more than 10%, then MD is 1 if greater than the value of N at 10%, then MD is equal to 2, if less than the value of N at 10%, then MD is 0 if the contents of gidroperekisi deviates from normal values not more than 10%, the GPU is 1 if greater than the value of N is 10%, SE = 2, if less than the value of N at 10%, SE is set to 0, if the content of diene conjugates deviates from normal values not more than 10%, then DK is 1 if greater than the value of N at 10%, then DK is equal to 2, if less than the value of N at 10%, then DK is equal to 0, if the content of vitamin E deviates from normal values is not more than 10%, BE equal to 1, if it exceeds the value of the N at 10%, then BE equal to 2, if less than the value of N at 10%, then BE equal to 0, and the value of KP is equal to 1 point, if the total value of points corresponding to the contents of ceruloplasmin (C, mg/100 g), malondialdehyde (MD, nmol/g), gidroperekisi (SE, nmol/g), diene conjugates (DC, nmol/g) and Vitamin E (BE, µg/g) in blood plasma, is from 3 to 6, and for values smaller or larger named limit, the value of KP is equal to 0 points, if the content of ceruloplasmin deviates from normal values not more than 10%, then C is 1 if greater than the value of N at 10%, then C is equal to 2, if less than the value of N at 10%, C is 0, if the content of malondialdehyde deviates from normal values not more than 10%, then MD is 1 if greater than the value of N at 10%, then MD is equal to 2, if less than the value N 10%MD is 0 if the contents of gidroperekisi deviates from normal values not more than 10%, the GPU is 1 if greater than the value of N is 10%, SE = 2, if less than the value of N at 10%, SE is set to 0, if the content of diene conjugates deviates from normal values not more than 10%, then DK is 1 if greater than the value of N at 10%, then DK is equal to 2, if less than the value of N at 10%, DK is equal to 0, if the content of vitamin E deviates from the normal value is not more than 10%, then BE equal to 1 if greater than the value of N is 10%, BE equal to 2, if less than the value of N at 10%, then BE equal to 0, and the value e is equal to 1 point, if the total value of points corresponding to the contents of malondialdehyde (MD, nmol/g), diene conjugates (DC, nmol/g) and vitamin E (BE, nmol/ml) in erythrocytes, is in the range from 1 to 4, and for values smaller or larger above limit, the value of e receive 0 points if the content of malondialdehyde deviates from normal values not more than 10%, then MD is 1 if greater than the value of N at 10%, then MD is equal to 2, if less than the value of N at 10%, then MD is 0, if the content of diene conjugates deviates from normal values not more than 10%, then DK is 1 if greater than the value of N at 10%, then DK is equal to 2, if less than the value of N at 10%, DC 0, if the content of vitamin E deviates from normal values not more than 10%, BE equal to 1 if greater than the value of N is 10%, BE equal to 2, if less than the value of N at 10%, then BE equal to 0, and the value T is equal to 1 point, if the total value of points corresponding to the contents of malondialdehyde (MD, nmol/g), diene conjugates (DC, nmol/g) and vitamin E (BE, nmol/ml) in platelets, is in the range from 1 to 4, and when the value is, smaller or larger above limit, the value of the index T is equal to 0 points, if the content of malondialdehyde deviates from normal values not more than 10%, then MD is 1 if greater than the value of N at 10%, then MD is equal to 2, if less than the value of N at 10%, then MD is 0, if the content of diene conjugates deviates from normal values not more than 10%, then DK is 1 if greater than the value of N at 10%, then DK is equal to 2, if less than the value of N at 10%, then DK is equal to 0, if the content of vitamin E deviates from normal values not more than 10%, BE equal to 1 if greater than the value of N is 10%, BE equal to 2, if less than the value of N at 10%, then BE equal to 0.



 

Same patents:

FIELD: medicine.

SUBSTANCE: vaginal fluid is analysed. The vaginal secretion is collected by means of a common tampon placed in a vagina for 8-9 hours, further weighted; microbial metabolites are extracted in equiponderate amount of distilled water, and the extract is analysed by gas-liquid chromatography. If the vaginal discharge contain acetic acid more than 0.315 mg/g and total propionic and butyric acids ≤0.200 mg/g in an age group of 17 to 34 years, and acetic acid more than 0.210 mg/g with total propionic and butyric acids ≤0.120 mg/g in an age group of 35 to 48 years, nonspecific aerobic vaginitis is diagnosed.

EFFECT: more accurate diagnosis of nonspecific aerobic vaginitis.

4 ex

FIELD: medicine.

SUBSTANCE: method of evaluating immunogenicity of brucella strains includes enzyme-linked analysis of culture supernatant of peripheral blood cells for content of cytokines - tumour necrosis factor (TNF-α), interleukin-1β (IL-1β) and colony-stimulating factor (CSF), synthesized by mononuclear cells of peripheral blood in vitro without impact (spontaneous production) and under the impact of antigens of evaluated brucella strains (induced production) and determination of their immunogenicity by ratio of spontaneous and induced production of said cytokines, brucella strains are considered immunogenic if their antigens cause enhancing of TNF-α by 1-1.25, IL-1β by 2-2.50 and CSF by 3-3.70.

EFFECT: method improvement.

1 tbl

FIELD: medicine.

SUBSTANCE: sampling of patient's lacrimal fluid (LF) is performed, analysed reaction mixture (ARM), consisting of substrate and analysed lacrimal fluid (ALF) is prepared, where as substrate, collagen gel is used. In course of ARM preparation, ALF is mixed with collagen gel in ratio 1-1.5:1 and keep at room temperature until homogeneous mixture (said ARM) is obtained. After that said ARM is applied on a microscope slide, kept until complete drying up of the entire microscope slide surface, measuring the time of complete ARM drying up. Then, quantitative determination of ALF CA is carried out by means of preliminarily built calibration curve of dependence of time of complete drying up of standardised reaction mixture samples, each of which consists of substrate and collalisin solution with specified collagenolytic activity, on collagenolytic activity. Obtained earlier ALF CA value is compared with normal values and if value of collagenolytic activity is lower than 231.8 kU/ml, lower ALF CA is determined, if the value of said activity is 231.8-297.8 kU/ml, normal ALF CA is determined, and if the value of said activity is higher than 297.8 kU/ml, higher ALF CA is determined.

EFFECT: application of the method makes it possible to increase accuracy of LF CA determination, reduce duration of determination procedure, eliminate possibility of infecting people who are carrying out the analysis.

1 dwg, 3 tbl

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to oncology, and can be used for clinical effectiveness control in children with neuroblastomas. That is ensured by neo-adjuvant combination cytostatic therapy with peripheral vein blood sampling prior to and after each course of chemotherapy. Blood is examined for plasminogen and plasmin activity to calculate the relation of the first to the second. If the value increased after chemotherapy, a therapeutic clinical effect is predicted. If the value decreased or remained unchanged throughout two courses of chemotherapy, the absence of effect is predicted that is a basis for changing the cytostatics to provide an adequate treatment.

EFFECT: method provides assessing cancer invasiveness, its invasive and metastatic potential, detecting the patients with an expected therapeutic effect, good prognosis and the patients with no effect who require timely correction of anticancer therapy to provide prolonged and improved quality of life of the patients.

2 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: invention concerns a method for prediction of the efficacy of Infliximab inclusion in a conventional therapy of the patients with rheumatoid arthritis (RA). A patient's peripheral blood is examined for total lymphocyte content (TLC) expressing TNF-α-CD120a receptors, for levels of spontaneous and stimulated production of tumour necrosis factor alpha (TNF-α) and interleukin-6 (IL-6). It is followed by calculating indexes of PHA action on PBMC (peripheral blood mononuclear cells) TNF-α and IL-6 production by formulae respectively: IA1=Ast/Asp, IA2=Bst/Bsp, where IA1 is an index of PHA action on PBMC TNF-α production; Ast is a level of stimulated TNF-α production, pg/ml; Asp is a level of spontaneous TNF-α production, pg/ml; IA2 is an index of PHA action on PBMC IL-6 production; Bst is a level of stimulated IL-6 production, pg/ml; Bsp is a level of spontaneous IL-6 production, pg/ml. Provided IA1≤2.4 and IA2≤1.6 and TLC≥6% simultaneously, the high efficacy of Infliximab inclusion in the therapy of the RA patients is predicted.

EFFECT: invention allows reliable prediction of the efficacy of Infliximab inclusion in the conventional therapy of the RA patients.

1 ex

FIELD: medicine.

SUBSTANCE: method starts with blood sample alkalisation with 10% sodium hydroxide to pH 8-10; dichlorobromomethane is recovered from the sample by hexane extraction; the extract is separated centrifugally at 7000-7500 rpm and analysed with gas chromatography in a partition gas chromatograph with an electron capture detector, while dichlorobromomethane is measured by a calibration diagram.

EFFECT: high sensitivity and accuracy of the method for blood dichlorobromomethane measurement.

1 ex, 5 tbl

FIELD: medicine.

SUBSTANCE: excitory cells of the immune system are recovered that is followed with primary incubation of the cells with an investigated substance to produce a primary-incubation supernatant or mixed cells and supernatant; secondary incubation of the target cells with the supernatant or mixed cells and supernatant wherein the secondary-incubation target cells are understood as human tumour cells or cell lines of an oncogenetic origins; the target cells are analysed where the analysis is specified in a group including an expression analysis of specific proteins and an apoptosis and/or necrosis analysis.

EFFECT: improvement of the method.

14 dwg

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to orthopedics. In order to estimate state of bone tissue in case of immobilisation osteoporosis in a laboratory animal examined are homogenates: bone, muscular, bone marrow of any extremity and peripheral blood. Biochemical and integral parametres are determined. Five factor variables F1-F5 are calculated using values of biochemical and integral parametres, constant values of factor coefficients of biochemical parametres and free coefficients. After that calculated is the value of discriminant function, whose value is used to estimate bone state as normal or conclusion about presence of immobilisation osteoporosis is made.

EFFECT: method increases accuracy and efficiency, has high stability of immobilisation osteoporosis recognition.

1 ex, 3 tbl

FIELD: medicine.

SUBSTANCE: blood plasma is analysed for cytochrome oxidase activity, erythrocyte 2,3- biphosphoglycerate and lactic acid concentrations. It is followed by calculating an oxygenation coefficient K by formula: K=(C1+C2):A, where C1 is the erythrocyte 2,3- biphosphoglycerate concentration, mol/l; C2 is the erythrocyte lactic acid concentration, mol/l; A is plasma cytochrome oxidase activity, mol/l. The K value withint 1.0 <K≤3.0 enables to diagnose tissue hypoxia, while in case of the K values being 3.0<K≤5.0 cardiovascular hypoxia is diagnosed. If the oxygenation coefficient is 5.0 and more, blood hypoxia is diagnosed.

EFFECT: use of the method enables the differential diagnostics of blood, tissue and cardiovascular hypoxia.

3 ex

FIELD: medicine.

SUBSTANCE: method for prediction of duration of a recurrence-free interval in patients with rectal cancer consisting in the fact that the surgical removal of a peritumoural recurrence is followed with biochemical analysis of the concentration of polypeptide hormone prolactin, and its specified tissue value enables to determine duration of the recurrence-free interval.

EFFECT: method allows predicting duration of the recurrence-free interval following the removal of the recurrence.

1 tbl

FIELD: medicine, hepatology.

SUBSTANCE: one should detect the level of hepato-specific enzymes (HSE) in blood plasma, such as: urokinase (UK), histidase (HIS), fructose-1-phosphataldolase (F-1-P), serine dehydratase (L-SD), threonine dehydratase (L-TD) and products of lipid peroxidation (LP), such as: dienic conjugates (DC), malonic dialdehyde (MDA). Moreover, one should detect the state of inspecific immunity parameters, such as: immunoregulatory index (IRI) as the ratio of T-helpers and T-suppressors, circulating immune complexes (CIC). Additionally, one should evaluate the state of regional circulation by applying rheohepatography (RHG), the system of microhemocirculation with the help of conjunctival biomicroscopy (CB) to detect intravascular index (II). In case of increased UK, HIS levels up to 0.5 mcM/ml/h, F-1-P, L-SD, L-Td, LP products, CIC by 1.5 times, higher IRI up to 2 at the norm being 1.0-1.5, altered values of regional circulation, increased II up to 2 points at the norm being 1 point, not more one should diagnose light degree of process flow. At increased level of UK, HIS up to 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 1.5-2 times, increased IRI up to 2.5, altered values of regional circulation, increased II up to 3-4 points one should diagnose average degree of process flow. At increased level of UK, HIS being above 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 2 and more times, increased IRI being above 2.5, altered values of regional circulation, increased II up to 5 points and more one should diagnose severe degree of process flow.

EFFECT: higher accuracy of diagnostics.

3 ex

FIELD: medicine, infectology, hepatology.

SUBSTANCE: in hepatic bioptate one should detect products of lipid peroxidation (LP), such as: dienic conjugates (DC), activity of antioxidant enzymes, such as: catalase (CAT)and superoxide dismutase (SOD). One should calculate by the following formula: C = DC/(SOD x CAT)x100, where DC - the content of dienic conjugates, SOD - activity of superoxide dismutase, CAT - activity of catalase. At coefficient (C) values being above 65 one should predict high possibility for appearance of cirrhosis, at 46-645 - moderate possibility and at 14-45 -low possibility for appearance of cirrhosis.

EFFECT: higher accuracy of prediction.

3 ex

FIELD: medicine, clinical toxicology.

SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.

EFFECT: higher accuracy of prediction.

2 ex, 3 tbl

FIELD: medicine, juvenile clinical nephrology.

SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.

EFFECT: higher efficiency of detection.

7 dwg, 1 ex, 6 tbl

FIELD: clinical medicine, pulmonology.

SUBSTANCE: one should carry out complex estimation of interleukin-1β) concentration in blood, saliva, bronchoalveolar liquid. Moreover, one should detect distribution coefficient (DC) for IL-1β as the ratio of IL-1β blood content to IL-1β salivary content. At increased IL-1β blood content by 10 times and more, by 2 times in saliva, unchanged level of bronchoalveolar IL-1β, at DC for IL-1β being above 1.0 one should predict bronchial obstruction. The method enables to conduct diagnostics of the above-mentioned disease at its earlier stages.

EFFECT: higher efficiency of prediction.

2 tbl

FIELD: medicine, diagnostics.

SUBSTANCE: the present innovation deals with genetic trials, with diagnostic field of oncological diseases due to analyzing DNA by altered status of gene methylation that take part in intracellular regulation of division, differentiating, apoptosis and detoxication processes. One should measure the status of methylation in three genes: p16, E-cadherine and GSTP1 in any human biological samples taken out of blood plasma, urine, lymph nodes, tumor tissue, inter-tissue liquid, ascitic liquid, blood cells and buccal epithelium and other; one should analyze DNA in which modified genes of tumor origin or their components are present that contain defective genes, moreover, analysis should be performed due to extracting and purifying DNA out of biological samples followed by bisulfite treatment of this DNA for modifying unprotected cytosine foundations at keeping 5-methyl cytosine being a protected cytosine foundation followed by PCR assay of bisulfite-treated and bisulfite-untreated genes under investigation and at detecting alterations obtained according to electrophoretic result of PCR amplificates, due to detecting the difference in the number and electrophoretic mobility of corresponding fractions at comparing with control methylated and unmethylated samples containing normal and hypermethylated forms of genes one should diagnose oncological diseases. The method provides higher reliability in detecting tumors, detection of remained tumor cells after operation.

EFFECT: higher efficiency of therapy.

1 cl, 3 dwg, 4 ex

FIELD: medicine, gastroenterology.

SUBSTANCE: one should carry out diagnostic studying, moreover, on the 5th -6th d against the onset of exacerbation in case of gastric and duodenal ulcerous disease one should detect the content serotonin, histamine and acetylcholine in blood, then during 2-3 wk one should conduct medicinal therapy to detect serotonin, histamine and acetylcholine level in blood again and at serotonin content being by 2-3 times above the norm, histamine - by 1.15-1.4 times above the norm and acetylcholine - by 20-45% being below the norm one should predict the flow of gastric and duodenal ulcerous disease as a non-scarring ulcer.

EFFECT: higher accuracy of prediction.

3 ex

FIELD: medicine.

SUBSTANCE: method involves taking blood from ulnar vein (systemic blood circulation) and from large vein of the injured extremity proximal with respect to lesion focus (regional blood circulation). Spontaneous NST-test value is determined and difference is calculated in systemic and regional blood circulation as regional-to-systemic difference. The difference value is used for predicting clinical course of pyo-inflammatory disease in extremities.

EFFECT: high accuracy of diagnosis.

4 cl, 2 tbl

FIELD: medicine, gastroenterology.

SUBSTANCE: one should introduce biologically active substance, moreover, in patient's blood serum one should detect the content of acetyl choline and choline esterase activity followed by 2-h-long intragastric pH-metry at loading with biologically active substance as warm 40-45%-honey water solution at 35-40 C, and at increased content of acetyl choline being above 1.0 mM/l, choline esterase being above 0.5 mM/l/30 min and pH level being 6.0-6.9 it is possible to consider apitherapy to be useful for treating ulcerous duodenal disease.

EFFECT: higher efficiency and accuracy of detection.

3 ex

FIELD: medicine, gastroenterology.

SUBSTANCE: it has been suggested a new method to detect pharmacological sensitivity to preparations as acidosuppressors. After the intake of the preparation a patient should undergo fibrogastroduodenoscopy 3 h later, then, through endoscopic catheter one should introduce 0.3%-Congo red solution intragastrically and the test is considered to be positive at keeping red color that indicates good sensitivity to the given preparation, and in case of dark-blue or black color the test is considered to be negative that indicates resistance to this preparation. The suggested innovation widens the number of diagnostic techniques of mentioned indication.

EFFECT: higher efficiency of diagnostics.

2 ex

Up!