Method to forecast development of toxic form of dyspepsia in newborn calves with initial symptoms of dyspepsia
FIELD: veterinary science.
SUBSTANCE: in newborn calves with initial dyspepsia symptoms blood samples are taken to identify aggregative activity of platelets using solutions of ADP, collagen, ristomycin and adrenalin as inducers by mixing of 0.1 ml of plasma rich in platelets and standardised by platelets quantity on a slide plate with 0.1 ml of one of aggregating agents and identifying time of platelet aggregates appearance in the form of whitish grain visible with the naked eye. The reference is the average result of identified aggregation of platelets in 10 healthy newborn calves. If platelets aggregation acceleration over 7% compared to the reference is detected in newborn calves with initial dyspepsia symptoms, development of toxic dyspepsia is forecasted. Early forecasting of toxic dyspepsia development in newborn calves has signification practical value, since it makes it possible to more thoroughly treat animals.
EFFECT: reduced loss of calves sick with dyspepsia and improved economic efficiency of stock raising.
1 tbl, 2 ex
The invention relates to veterinary medicine, namely, to the diagnosis of animal diseases.
It is now established that in certain pathological conditions of animals to observe changes in the aggregation activity of platelets.
There are various methods of determining the functional capacity of platelets, based on the assessment of spontaneous or induced (using various agonists) platelet aggregation (Barkagan SS, Momot A.P. Diagnostics and controlled therapy of disorders of hemostasis. - M.: "Novamed-JSC, 2001. - 296 S.).
A known method of determining aggregation activity of platelets on glass" (Shitikova AS. Visual micromethod determine the aggregation activity of platelets with various aggregating agents / Ashitkov // Laboratory work. - 1984. No. 4. - S-220).
However, all these methods are only used to assess the functional activity of platelets for various diseases, and to predict the development of toxic forms of dyspepsia are not applied.
The purpose of the invention is the prediction of newborn calves with an initial signs of dyspepsia development of toxic forms of dyspepsia.
Early prediction of the development of toxic dyspepsia newborn calves is of great practical importance because it allows a more thorough approach to t is rapee and timely preventive treatment of sick animals, that will help reduce the mortality of cases of dyspepsia newborn calves and raising the economic efficiency of livestock.
The essence of the method consists in the following. Newborn calves with an initial signs of dyspepsia take samples of blood and determine aggregation activity of platelets using as inductors solutions ADP, collagen, ristomycin and adrenaline by mixing on a slide 0.1 ml of standardized platelet count platelet-rich plasma and 0.1 ml of one of the aggregating agents, and identify the time of manifestation of platelet aggregates in the form of whitish grains visible to the naked eye. The control used is the average result of the determination induced platelet aggregation in 10 clinically healthy calves. In the detection of newborn calves with an initial signs of dyspepsia acceleration of platelet aggregation over 7% of control it is possible to predict the development of toxic dyspepsia.
The method is as follows: newborn calves with an initial signs of dyspepsia make sampling blood in the morning before feeding in plastic dishes. To prevent spontaneous aggregation apply the stabilization of the obtained blood of 3.8% solution of sodium citrate is a ratio of 1:4. Define induced aggregation activity of platelets using as inductors solutions ADP (breeding stock solution 1:10), collagen (concentration 20 mg/ml), ristomycin (concentration 15 mg/ml) and epinephrine (dilution of stock solution 1:50). From stabilized blood receive platelet-rich plasma and platelet-poor plasma. Used platelet-rich plasma obtained by centrifugation of the obtained stable blood at 1000 rpm for 6 min; and plasma poor in platelets, after centrifugation the remaining in vitro platelet-rich plasma at 3000 rpm for 20 minutes platelet-Rich plasma standardise the number of platelets by dilution, if necessary, autologous sample of platelet-poor plasma (up to 380-400000 platelets/µl). The volume mix is rich and platelet-poor plasmas is determined by the formula (1):
where Vppp- the volume of platelet-poor plasma,
Vprp- the volume of platelet rich plasma,
N is the number concentration of platelets in the original platelet-rich plasma (cells/µl).
Further, different pipettes for non-fat dry glass slide put 0.1 ml of the investigated platelet-rich plasma and 0.1 ml of one of the aggregating agents. In the Les of mixing with a glass rod drops on a glass slide immediately include a timer and set the time of manifestation of the so-called phenomenon of the snow storm when aggregates of platelets reach such sizes that can be visible to the naked eye as whitish grains (required the use of light microscope type OI-19). The phenomenon of snow storms in healthy individuals occurs at the same time, while strengthening the aggregation function it appears earlier standards, with the weakening later. Response repeat 2 times with the same aggregation agent. The results take into account in seconds. The result is considered average from the two definitions of the values.
The control used is the average result of the determination by the above method induced platelet aggregation in 10 clinically healthy calves of the same age group.
In the detection of newborn calves with an initial signs of dyspepsia acceleration of platelet aggregation over 7% of control it is possible to predict the development of toxic forms of dyspepsia.
Example 1. According to the principle of analogues with regard to the origin, age and body weight at birth were formed 2 groups of newborn calves. Terms of housing, feeding and care of animals were the same. The first group consisted of 22 clinically healthy calves. The second group was composed of 17 calves with initial symptoms of dyspepsia were treated according to the sh farm is IU (tylosin-50 0.2 ml/kg intramuscularly 1 time per day until recovery; the broth sorrel isotonic sodium chloride solution inside the morning and evening at 0.5 l). During the experiment the second group was divided into two subgroups: patients with simple (n=10) and patients with a toxic form of dyspepsia (n=7) calves. The criterion for this division was the difference in the clinical picture of the disease. For calves with simple dyspepsia was characteristic not too rapid defecation emitting liquefied feces, the General condition of patients calves remained satisfactory. In calves, sick toxic form of dyspepsia, with full development of the disease pattern was observed profuse diarrhea, were observed depression, weakness, emaciation, therasense coat, dryness of nasal mirror.
In patients with dyspepsia calves (n=17) platelet aggregation was determined in the beginning of the disease (when the first signs of dyspepsia), in the midst of disease (at 2-3 days dyspepsia) and stage of recovery.
The results of determining induced platelet aggregation in clinically healthy and patients with dyspepsia calves are presented in table 1. As can be seen from table 1, patients with dyspepsia newborn calves at the beginning of dyspepsia in comparison with the average data of clinically healthy calves occurred acceleration induced aggregation on 5,51% when using collagen, 6,26% when s is the physical alteration of ADP, on 5,95% when using ristomycin and 5,80% when using adrenaline. When toxic dyspepsia early in the disease the results of the aggregation was ahead of the average of the healthy newborn calves on 7,72% when using collagen, 8,39% when using the ADF, of 7.82% in ristomycin and 8,09% when using adrenaline.
|Induced platelet aggregation (AT) clinically healthy and patients with dyspepsia newborn calves|
|Inductors||Average AT a healthy newborn calves (n=22)||Simple dyspepsia (n=10)||Toxic dyspepsia|
|Onset||The height of the disease||Stage recovery||The onset of the disease (n=7)||The height of the disease (n=7)||Stage of recovery(n=5)|
|ADP,||38,97±1,61||36,53±0,39*||32,94±0,07*||37,06±0,07*||35.70 barm±0,40*||to 23.03±0,06*||34,13±0,14*|
|* -p≤0.05 compared with the average AT a healthy newborn calves|
Accordingly, already at the early symptoms of dyspepsia, when it is impossible to diagnose the disease, platelet aggregation occurs before more than 7% of those newborn calves, which further perebolevayet toxic form of dyspepsia. That is they way the definition of induced platelet aggregation in the newborn calves on the proposed methodology can be used as a relatively simple and readily available in economy a method for predicting the development of toxic forms of dyspepsia in newborn calves with an initial signs of dyspepsia.
Example 2. The newborn calf No. 122 identified the initial symptoms of dyspepsia: not too rapid defecation emitting liquefied feces. To evaluate the risk of toxic forms of dyspepsia the animal selected blood sample, defined aggregation activity of platelets using as an inducer of collagen. This figure from a diseased calf equal 27,25 C. as a control used the average result of the definition of the aggregation activity of platelets in 10 clinically healthy calves (30,05). The acceleration of platelet aggregation from a diseased calf is 2,80 with or to 9.32%. Accordingly, this animal can predict the development of toxic forms of dyspepsia. The calf assigned adequate therapy: 0.1 ml of 0.1%solution of the drug "Biopag-D" 1 kg live weight of calves 2 times a day orally with colostrum-milk; 0.2 g/kg animal body weight of enterosorbent "Polisorb-EP" inside in the form of an aqueous suspension for 1 hour before giving colostrum or 2 hours after feeding; roopali who Liukin with glucose 5 ml per 1 kg body weight of 1 times a day intravenously; 50 ml of isotonic sodium chloride solution on the head intravenous 1 time per day. In the result, the animal had indigestion in the form of light.
1. A method for predicting the development of toxic forms of dyspepsia in newborn calves with an initial signs of dyspepsia, which includes the determination of the aggregation activity of platelets using as inductors solutions ADP, collagen, ristomycin and adrenaline by mixing on a slide 0.1 ml of standardized platelet count platelet-rich plasma and 0.1 ml of one of the aggregating agent, and determining the time of manifestation of platelet aggregates in the form of whitish grains visible to the naked eye, when the acceleration of platelet aggregation over 7% of control predict the development of toxic forms of dyspepsia.
2. The method according to claim 1, characterized in that the control used is the average result of the determination induced platelet aggregation ten clinically healthy calves.
SUBSTANCE: invention refers to medicine, particularly to gastroenterology. Diagnosing active opium addiction in patients with chronic viral hepatitis type C is ensured by evaluating: basal, stimulated level and stimulation index of tumour necrosis factor α (TNF-α); basal and stimulated level of interferon-γ (IFN-γ); stimulation index of interleukin-2 (IL-2); basal level of interleukin-4 (IL-4) If basal TNF-α is 12 pg/ml to 50 pg/ml, stimulated TNF-α is 15 pg/ml to 80 pg/ml, TNF-α stimulation index is 0.65 to 1.78, basal IFN -γ is 20 pg/ml to 22 pg/ml, stimulated IFN -γ is 20 pg/ml to 22 pg/ml, stimulation index of IL-2 is 1 to 1.45, basal IL-4 is 9.1 pg/ml to 11.8 pg/ml, active opium addiction is diagnosed.
EFFECT: technique provides higher accuracy of diagnosing active opium addiction in patients with chronic viral hepatitis type C.
3 ex, 1 tbl
SUBSTANCE: invention refers to medicine. A matrix mass spectrometry technique is used to detect the presence of a residue in an imprint. A powder containing hydrophobic particles of silicon dioxide, as well as a metal, metal nitride, a metal oxide or carbon is applied on the imprint. An average particle diametre of said metal, metal nitride, metal oxide or carbon introduced in the hydrophobic particles of silicon dioxide is 65 to 90 mcm or 400 to 500 nm preferentially. The investigated imprint is lifted from the application area with using a lifting tape and brought in contact with a sample substrate of a mass spectrometre, then the powder is applied on the imprint. The powder is magnetic or paramagnetic and additionally contains a fluorescent or pigmented dye molecule. The mass spectrometry is conducted with using a matrix specified in a group including MALDI-TOF-MS-MS or SALDI-TOF-MS-MS and their combinations. The residue represents an endogenous residue, particularly endogenous and/or exogenous metabolites and a contact residue. The endogenous metabolite represents squalene, while the exogenous metabolite is nicotine metabolite, e.g. cotinine, and the contact residue is the drug cocaine.
EFFECT: method allows executing the imprint residue analysis on a human imprint directly that eliminates it to be replaced.
21 cl, 14 dwg
SUBSTANCE: sodium fluoride is added to an analysed sample in amount of 10% of the mass of the biological object and infused twice in 45 minutes with portions of ethyl acetate, the mass of each of which is twice higher than the mass of the biological material. Separate extractions are combined, filtered through anhydrous sodium sulphate. The solvent from the filtrate is evaporated at temperature 50-60°C. The residue is dissolved in a mixture of hexane-dioxane-propanol-2 solvents. Chromatography is performed in a column with silica gel L 40/100 µ using a hexane-dioxane-propanol-2 mobile phase. The eluate fractions which contain the analysed substance are merged. The eluate is evaporated. The residue is dissolved in a mixture of hexane-dioxane-propanol-2 solvents and the analysed substance is determined via high performance liquid chromatography (HPLC) in a 64x2 mm column filled with Silasorb 600 sorbent using a hexane-dioxane-propanol-2 mobile phase and a UV detector.
EFFECT: invention shortens the duration of detecting tetraethyl thiuram disulphide in blood and increases its sensitivity.
3 tbl, 2 ex
SUBSTANCE: in patients with progressive myopia if glaucoma suspected, lachrymal fluid is examined for the concentration lactic (LA) and pyruvic (PA) acids for the right and left eyes. Then for each eye, hypoxia coefficients are calculated by formulae: HCOD=LAOS/PAOD, HCOS=LAOS/PAOS, where HCOD is the hypoxia coefficient of the right eye; LAOS is the LA concentration in the LF of the right eye, mmol/l; PAOD is the PA concentration in the LF of the right eye, mcmol/l; HCOS is the hypoxia coefficient of the left eye; LAOS is the LA concentration in the LF of the left eye, mmol/l; PAOS is the PA concentration in the LF of the left eye, mcmol/l. If the hypoxia coefficient HCOD exceeds 1.0, glaucoma on the corresponding eye is diagnosed.
EFFECT: use of the technique enables high reliable diagnosis of glaucoma in patients with progressive myopia.
SUBSTANCE: diagnostic technique for chronic viral hepatitis type C associated with opium addiction consisting in needle biopsy of a liver that is followed by optical microscopy to analyse the prepared biopsy materials for a basal, stimulated level of interleukine-10 (IL-10), and if observing the basal IL-10 within 218.5 pg/ml to 288.5 pg/ml, and the stimulated IL-10 within 468 pg/ml to 588 pg/ml, the presence of fibrosis of the central vein, then chronic viral hepatitis type C associated with opium addiction is diagnosed.
EFFECT: improved diagnostic accuracy.
2 tbl, 3 ex
SUBSTANCE: invention refers to medicine, namely to diagnosing malignant processes in a human body. A diagnostic technique for a malignant process in a human body consisting in sampling a patient's living tissue, grinding, mixing with physiologic saline to a state of suspension, keeping and agitating under certain conditions, then centrifuging, separating supernatant and detecting cancer-specific markers by an immunochemiluminisent assay.
EFFECT: method exhibits a simple and high degree of malignant process detection in the human body, both at the early, and following stages of disease.
SUBSTANCE: method of hepatic fibrosis assessment in patients with chronic viral hepatitis type C consisting in evaluating CB56+ phenotype in blood lymphocytes with the value range of blood CD3+/CD56+, CD3+/CD56+/CD4+, CD3+/CD56+/CD8+, CD56+/CD94+, CD56+/NKG2D+, CD56+/CD107a+ cell percentage showing third stage (pre-cirrhotic) or cirrhosis.
EFFECT: method allows higher diagnostic accuracy in fibrous hepatic changes.
7 dwg, 3 tbl, 3 ex
SUBSTANCE: determining individual genome sensitivity to radon exposure is ensured by genetic blood test to identify predisposing and protective genotypes: marker Arg280His of gene XRCC1 - predisposing genotype Arg/Arg, protective genotype Arg/His; marker Argl94Trp of gene XRCC1 - predisposing genotype Arg/Arg, protective genotype Arg/Trp; marker Asnl48Glu of gene APE1 - predisposing genotype Glu/Glu, protective genotypes Asn/Asn, Asn/Glu; marker A2455G of gene CYP1A1 - predisposing genotype A/G, protective genotypes A/A and G/G; a deletion marker in gene GSTM1 - predisposing genotype o/o, protective +. High individual sensitivity to high radon dose is stated by observing the quantitative prevalence of predisposing genotypes or the equal quantity of predisposing and protective genotypes. High individual resistance to high radon dose is determined by the quantitative prevalence of protective genotypes.
EFFECT: use of the method allows estimating genetically determined predisposition to formation of high level of chromosomal aberrations even before exposing to a radiating factor.
5 tbl, 2 ex
SUBSTANCE: patient examination involves visual evaluation of a periodontal pocket depth, a tooth mobility degree, gum tissue state with using periodontal indexes. X-ray examination aims at evaluating an alveolar bone resorption degree. It is followed with biochemical blood analysis for the parathormone and calcitonin concentrations. If simultaneously observing the blood calcitonin concentration less than a lower physiological norm, while the parathormone concentration exceeding an upper physiological norm, the presence of chronic aggressive generalised periodontitis is concluded.
EFFECT: use of the technique allows advanced detection of chronic aggressive generalised periodontitis, early identification of said disease among the other inflammatory diseases of periodontium at initial morbidities.
3 dwg, 2 tbl, 2 ex
SUBSTANCE: biological tissue is crushed, processed twice for 30 minutes with portions of ethyl acetate, weight of each twice exceeding weight of a biological object; prepared extractions are combined, filtered through anhydrous sodium sulphate; a solvent is evaporated from the filtrate; the residue is dissolved in acetonitrile; the prepared solution is watered down in the volume ratio 1:4, extracted twice in portions of chloroform, volume of each being equal to volume of a hydrophilic layer; the chloroform extracts are combined, steamed to a dry residue; the residue is dissolved in mixed solvents hexane-dioxane-propanol-2, cleaned in a silica gel column L 40/100µ with using a mobile phase hexane-dioxane-propanol-2; eluate fractions containing an analysed substance are combined; the eluent is evaporated; the residue is dissolved in mixed solvents hexane-dioxane-propanol-2 and analysed by a HELC method in a column of dimensions 64×2 mm filled with the sorbent Silasorb 600 with using a mobile phase hexane-dioxane-propanol-2 and a UV detector.
EFFECT: invention allows higher selectivity, sensitivity and accuracy of biological material analysis for tetramethylthiuramdisulfide.
4 ex, 5 tbl
FIELD: medicine, psychiatry.
SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
EFFECT: more objective prediction of disease development.
FIELD: medicine, urology.
SUBSTANCE: one should conduct subcutaneous prevocational tuberculin test and, additionally, both before the test and 48 h later it is necessary to perform the mapping of prostatic vessels and at decreased values of hemodynamics one should diagnose tuberculosis. The information obtained should be documented due to printing dopplerograms.
EFFECT: more reliable and objective information.
1 ex, 1 tbl
FIELD: molecular biology.
SUBSTANCE: the suggested innovation deals with the fact that nucleic acids should be isolated directly out of the sample without pipetting stage but with the help of interconnected reservoirs being prepared beforehand. The above-mentioned vessels should be applied either separately or being interconnected according to standard microtitrating format. The sample should be mixed with a lyzing buffer and nucleic acids are bound with matrix in closed system including, at least, two interconnected reservoirs. Forced movement of sample's mixture and buffer back and forth from one reservoir into another one for several times through narrow passage provides their thorough intermixing. The method provides quick and safe isolation of nucleic acids.
EFFECT: higher efficiency.
44 cl, 4 dwg, 1 ex
FIELD: medicine, phthisiology, microbiology.
SUBSTANCE: diagnostic material is poured preliminary with chlorohexidine bigluconium solution, homogenized, kept at room temperature for 10-12 h and centrifuged. Precipitate is poured with Shkolnikova's liquid medium, incubated at 37oC for 3 days, supernatant part of Shkolnokova's medium is removed, fresh Shkolnikova's medium is added, and precipitate is stirred and inoculated on the dense cellular egg media. Sensitivity of the strain is determined in 3 weeks by the presence of growth in the control tube only. Invention provides enhancing precision and reducing time for assay. Invention can be used in assay for medicinal sensitivity of tuberculosis mycobacterium.
EFFECT: improved assay method.
FIELD: medicine, biotechnology, pharmacy.
SUBSTANCE: invention relates to agents used for treatment of pathological states associated with disorder of synthesis of neuromediating substances. Method involves the development of pharmaceutical composition and a method for it preparing. Pharmaceutical composition represents subcellular synaptosomal fractions: synaptic membranes, "light" synaptosomes and "heavy" synaptosomes prepared from gray matter of cerebral hemispheres from experimental animals based on the goal-seeking modification of humoral mediators of nerve endings transformed to synaptosomes in development and regression of malignant processes. The composition provides inhibiting the growth of tumor cells, to elevate span-life of patients with ascite Ehrlich's sarcoma, breast adenocarcinoma Ca-755, Wolker's carcinosarcoma-256.
EFFECT: valuable medicinal and anti-tumor properties of composition.
12 cl, 3 tbl, 3 ex
SUBSTANCE: method involves carrying out microscopic examination of blood serum samples taken from femoral vein and cubital vein. Femoral vein sample is taken on injured side. The examination is carried out before and after treatment. The blood serum samples are placed on fat-free glass slide in the amount of 0.01-0.02 ml as drops, dried at 18-30°C for 18-24 h. The set of pathological symptoms becoming larger or not changed after the treatment in comparison to sample taken before treatment, and morphological picture of samples under comparison taken from the cubital vein showing no changes or being changed to worse, the treatment is considered to be effective.
EFFECT: enabled medicamentous treatment evaluation in course of treatment to allow the treatment mode to be changed in due time; avoided surgical intervention (amputation); retained active life-style of aged patients.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
FIELD: medicine, juvenile clinical nephrology.
SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
EFFECT: higher efficiency of detection.
7 dwg, 1 ex, 6 tbl
FIELD: medicine, urology.
SUBSTANCE: the present innovation deals with differential diagnostics of prostatic cancer and other prostatic diseases at the stage of primary inspection. The method includes the detection of PCA and calculation of probability coefficient for prostatic cancer (PCC) by the following formula: where e - the foundation of natural logarithm (e=2.718…), PCA - the level of total blood PCA in ng/ml, V - patient's age in years. At PCC value being above 0.2 one should diagnose prostatic cancer and to establish final diagnosis one should perform polyfocal prostatic biopsy. The method enables to increase accuracy of diagnostics at decreased number of unjustified prostatic biopsies.
EFFECT: higher efficiency of diagnostics.
FIELD: medicine, biology.
SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.
EFFECT: improved an valuable properties of nutrient medium.