Method for prediction of atypical antipsychotic drug treatment rates in shizophrenics
SUBSTANCE: clinical investigation is supplemented with simultaneously testing monoamine oxidase (MAO) activity in thrombocytes in nmole of benzaldehyde per 1 mg of protein an hour and semicarbazide-dependent amine oxidase (CAO) activity in blood serum in nmole of benzaldehyde per one ml of blood serum an hour. The blood serum CAO to thrombocyte MAO activity relation is calculated. If the value exceeds 0.6, a positive result of atypical antipsychotic drug treatment in schizophrenia is predicted.
EFFECT: more precise and objective prediction, reduced length of treatment in schizophrenics.
The problem of predicting the effectiveness of pharmacotherapy (CFT) before treatment is relevant in connection with a large selection of expensive psychotropic drugs and the individual response of patients.
There is a method of predicting the effectiveness of CFT by clinical studies on therapy after 3-6 weeks after beginning treatment (Ph.D GY, Neduva A.A. Treatment of mental patients. - M.: Medicine, 1988, p.8-25).
The disadvantage of this method is the duration of the study 3-6 weeks, as well as the subjective evaluation of the patient depending on the practitioner.
There is a method of predicting the effectiveness of treatment residual schizophrenia by clinical and biological research - determining the concentration of neurosteroid dehydroepiandrosterone, cortisol and rate of apoptosis of neutrophils (RU patent No. 2319155 C2, 10.03.2008). The disadvantage of this method is time consuming, expensive research.
The technical result of the proposed solutions is empowering predicting treatment of atypical antipsychotics schizophrenia patients, the reduction treatment, saving medicines, reduction of treatment time.
This result is achieved by the fact that in addition to the clinical study to determine simultaneously the activity is monoaminoxidase (MAO) in platelets of 1 nmol of benzaldehyde per mg protein per hour and semicarbazides oxidase (CAO) in the serum of 1 nmol of benzaldehyde/ml of serum per hour, calculate the ratio of activity of CAO to the activity of MAO and with the value of more than 0.6 predict a positive outcome of schizophrenia atypical antipsychotics.
The method is as follows.
Patients with schizophrenia before treatment in addition to the clinical study to determine simultaneously the activity of monoamine oxidase in platelets in nmol benzaldehyde 1 mg protein per hour according to the method described in the article Ontologies, Taskswitching Method of determining monoaminoxidase activity of platelets, Zh. "Laboratory work", 1985, No. 5, p.289-291 and activity semicarbazides oxidase in serum at a concentration of benzaldehyde in 1 ml per hour according to the method described in the article Ahisaariego Colorimetric method for determining the activity of monoamine oxidase in serum // Ukr. "Laboratory work", 1976, No. 3, s-153. Calculate the ratio of activity semicarbazides oxidase in serum activity of monoamine oxidase in platelets and when the value of the ratio more than 0.6 predict a positive outcome of therapy atypical antipsychotics schizophrenia patients.
Assessment of mental status is carried out by the PANSS (Kay, S.R., Fiszbein a, Opler LA The positive and negative syndrome scale (PANSS) for schizophrenia // Schizophr Bull. 1987. Vol 13, No. 2. P.261-276.
Patient M., case History No. 105. The tread is in the clinic UHF IRI psychiatry University, 19.01.2007 Diagnosis: paranoid Schizophrenia. The observation period less than one year. (ICD-10: F 20.0). Mentally ill for 2 years. During the previous two years had two supplications attack paranoid structures permitted without treatment within 3-4 months. Six months before the present admission gradually began to grow paranoid symptoms in the form of the ideas of the impact of mental automatisms, pseudohallucinations, brad relations. Describes the symptoms gradually increased in connection with what was referred for treatment in the clinic of the Institute. Before therapy is defined activity in serum CAO (10 nmol benzaldehyde in 1 ml per hour) and MAO activity (13,8 nmol benzaldehyde for 1 mg of protein per hour) in platelets, the value of which amounted to 0.7. Was conducted therapy atypical neuroleptic risperidone at a dose of 4 mg per day. Already during the first weeks of therapy had improvement of psychopathological symptoms. The patient was discharged with improvement. PANSS when entering 78 points, while the extract 60 points.
Thus, the ratio of CAO to MAO than 0.6 is a predictor of positive therapeutic prognosis in patient with schizophrenia in the treatment of atypical neuroleptic.
Patient N., Case history No. 1280. Was admitted to the hospital UHF IRI psychiatry University 09.10.2007, Di is the prognosis Schizophrenia paranoid. The observation period less than one year (ICD-10: F 20.0). The disease made its debut 3 years prior to referral to a psychiatrist. Psychopathological symptoms were determined hallucinatory version of Kandinsky syndrome-clérambault. PANSS when entering 83 points. Before therapy is defined activity in serum CAO (15.0 nmol benzaldehyde in 1 ml per hour) and MAO activity (9.0 nmol benzaldehyde for 1 mg of protein per hour) in platelets, the value of which amounted to 1.7 (15,0/9,0). In the treatment of atypical neuroleptic olanzapine noted positive dynamics in the form of the disappearance of hallucinatory product flows thoughts, streamlined the behavior that has an impact on social functioning. Issued in remission. PANSS - 53.
Thus, the ratio of CAO to MAO than 0.6 is a predictor of positive therapeutic prognosis in a patient with schizophrenia in the treatment of atypical neuroleptic.
The proposed method is applied 36 patients with schizophrenia. The coincidence of the clinical and biochemical parameters in 73,3% of patients (p<0,001).
This method has advantages compared with the known, consisting in the reduction of treatment time, increase accuracy, objectivity diagnosis and saving drugs.
A method for predicting the effectiveness of treatment atipic the diversified antipsychotics schizophrenia by clinical and biological research, characterized in that in addition to the clinical study to determine simultaneously the activity of monoamine oxidase (MAO) in platelets in nmol of benzaldehyde per 1 mg of protein per hour, and the activity semicarbazides oxidase (CAO) in the serum at the concentration of benzaldehyde per ml of serum per hour, calculate the ratio of the activity of CAO in the serum to the activity of platelet MAO, and when the value of more than 0.6 predict a positive outcome in the treatment of schizophrenia with atypical neuroleptics.
SUBSTANCE: inventions concern methods and analyses for studying expression of one or more biomarkers in a mammal's tissue or cell sample; study kits and products are presented also. Identifying expression of GalNac-T14 molecules predicts sensitivity or specifies that the tissue or cell sample is supposed to be sensitive to apoptosis inducing agents, such as Apo2L/TRAIL.
EFFECT: information obtained by the analysis aiming at identifying GalNac-T14 expression in the tissue or the cell sample in a mammal can provide an attending physician with the information which can be used for prescribing an optimum treatment regimen for the patients suffering such diseases as pancreatic cancer, lymphoma, non-small cell carcinoma of lung, colon cancer, rectal cancer, melanoma or chondrosarcoma.
22 cl, 50 dwg, 6 tbl
SUBSTANCE: five-percent placental tissue extract is analysed for the concentration of arginine and histidine to be related, and if the relation is lower than 0.86, development of cerebral affection in a newborn is predicted.
EFFECT: more precise and specific prediction of perinatal CNS affection in the newborn and enabled well-timed pathogenetic therapy.
3 dwg, 1 tbl
SUBSTANCE: patient's blood serum is treated with 7% solution of polyethylene glycol-6000, incubated and with dye Sudan B at 40°C for 1 hour, separated electrophoretically in agarose gel. After that, additionally, before treatment of blood serum with 7% PEG-6000 to 0.6 ml of sample 0.2 ml of 0.1% tritone X-100 solution is added, incubated for 15 minutes at 20°C, after which mixture is mixed by shaking 120 times per 1 minute. Application of method makes it possible to detect additional intensive minor fraction of modified LP(a).
EFFECT: increase of diagnostics accuracy.
3 dwg, 1 tbl
SUBSTANCE: method of determination of triterpene saponins in vegetable raw material and medications includes dissolution of saponin-containing fraction in mixture water-ammonium buffer, determination of its optic density and calculation of saponin content in terms of oleanolic acid, under specified conditions.
EFFECT: claimed method represents express method, facilitates analysis and increases degree of reliability of obtained results.
SUBSTANCE: in order to estimate efficiency of treating ischemic nephropathy in newborn babies in early neonatal period activity of gamma-glutamyltransferase and cholinesterase in child's urine is determined in dynamics of treatment. If activity of said enzymes decreases with respect to initial level, treatment is estimated as efficient, if activity increases or does not change - as inefficient.
EFFECT: application of method makes it possible to increase accuracy of estimation of ischemic nephropathy treatment in newborns, carry out correction of therapeutic measures in due time and improve disease outcome.
1 tbl, 3 ex
SUBSTANCE: in a first trimester of pregnancy, the microalbuminuria level is determined. If the value is 45 mg\l and more, placental insufficiency is predicted.
EFFECT: method enables early prediction of placental insufficiency by a simple quantitative estimation and thereby ensures early adequate preventive treatment.
SUBSTANCE: detecting expression of GalNac-T14 molecules enables to predict sensitivity or indicates that a tissue or cell sample is sensitive to apoptosis inducing agents, such as DR4 or DR5 agonist antibodies. The information obtained by the analysis aimed at detecting GalNac-T14 expression in the mammal's tissue or cell sample can provide a hospital doctor with data which can be used for prescribing an optimal treatment schedule for patients suffering such diseases as pancreas cancer, lymphoma, non-small cell carcinoma of lung, colon cancer, rectal cancer, melanoma or chondrosarcoma.
EFFECT: expanded scope of the compounds.
16 cl, 53 dwg, 14 tbl
SUBSTANCE: invention relates to field of medicine, in particular toxicology and resuscitation science and can be used for early prognosis of course of acute poisoning with psychotropic drugs. At the time of patient's admission to hospital albumin fraction of blood serum is isolated. After that, general level of reduced thiols is determined, and if its value is lower than 220 mcmol/l development of negative disease dynamics is predicted.
EFFECT: method allows to increase efficiency of performed treatment in said category of patients.
1 tbl, 8 ex
SUBSTANCE: invention refers to chlitin derivative photoproteins and to application thereof both as intracellular calcium indicators, and in cellular studies. Said proteins are produced by mutagenesis of a coding sequence of chlitin. Also, there are offered nucleic acids coding said protein, a vector containing said nucleic acids and a host cell carrying the vector. They can find application in genetic communication technologies for monitoring the cellular events associated with signal transmission and gene expression. Besides, photoproteins of the present invention can be used as intracellular calcium indicators in diagnostic techniques based on calcium concentration measurement in response to the various effects.
EFFECT: produced proteins exhibit enhanced bioluminescence, high affinity to calcium and prolonged light emission.
19 cl, 16 dwg, 5 ex
SUBSTANCE: in order to realise the method level of ferritin in blood serum and bile bladder tissue, rang point is defined, and ferritin values in serum from 0 to 10 ng/ml, and bile bladder from 0 to 0.25 mg/l - are taken for 1 point, respectively, level of ferritin in serum 70 ng/ml is taken as 7 points, and in bile bladder tissue 0.75 mg/l is taken as 3 point, and if sum is 10 points, conclusion is made about non-destructive cholecystitis, and if sum is from 10 points conclusion about destructive cholecystitis is made.
EFFECT: application of invention allows to increase accuracy of diagnostics of bile bladder tissue destruction in acute cholecystitis.
FIELD: medicine, ophthalmology.
SUBSTANCE: in lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula: At PC value being below 10.0 one should predict favorable disease flow, and at PC value being above 10.0 - unfavorable flow.
EFFECT: higher accuracy of prediction.
FIELD: forensic medicine.
SUBSTANCE: for the purpose to detect the sequence of applied lesions at availability of several wounds, scratches and ecchymoses on a cadaver one should study the activity of alkaline peptides isolated out of affected tissue by the impact of blood neutrophils of healthy donors upon phagocytosis. Moreover, the highest stimulating effect belongs to the peptides isolated out of the lesion applied earlier. The method enables to detect the sequence of applied lesions more accurately and differentiate the repeated lesion applied 5 min later, or more.
EFFECT: higher efficiency and accuracy of detection.
2 ex, 2 tbl
FIELD: medicine, biochemistry.
SUBSTANCE: in blood serum one should detect the level of lactoferrin and biliary acids. At their ratio being equal to 5-17 it is necessary to detect chronic hepatitis of high activity.
EFFECT: higher accuracy of detection.
SUBSTANCE: method involves determining cathepsin D activity in endometrium bioptate. The value being equal to or less than 0.1 units of enzymatic activity per hour, external genital endometriosis is diagnosed.
EFFECT: high accuracy of diagnosis.
SUBSTANCE: method involves studying lactoferrin content in blood serum and peritoneal exudates in postoperative period every day during the first three days. Lactoferrin concentration in blood serum being concurrently reduced by 0.02 mcmole/l or less and increasing lactoferrin concentration in peritoneal exudates by 0.04 mcmole/l or more, enteric detoxication is considered to be effective.
EFFECT: high quality of estimation.
SUBSTANCE: method involves determining plasminogen/plasmin, α2-macro-globulin, α1-antitripsin content at the first, third, fifth and tenth day. The plasminogen/plasmin level being equal to 66-74 mcmole/l or 100-120 mcmole/l, α2-macro-globulin level of 2.7-3.0 mcmole/l, α1-antitripsin content of 2.38-3.2 mcmole/l, systemic inflammatory response to purulent infection, light severity degree endotoxicosis is diagnosed and favorable disease outcome is predicted. The plasminogen/plasmin level being equal to 50-65 mcmole/l or 125-160 mcmole/l, α2-macro-globulin level of 2.3-2.6 mcmole/l, α1-antitripsin content of 3.3-4.0 mcmole/l, sepsis with organ and system dysfunction, moderate severity degree endotoxicosis is diagnosed and septic complication availability and lingering disease development course is predicted. The plasminogen/plasmin level being equal to 39-40 mcmole/l, α2-macro-globulin level of 1.58-2.08 mcmole/l, α1-antitripsin content of 5.0-6.2 mcmole/l, severe sepsis, septic shock, severe degree endotoxicosis is diagnosed and unfavorable disease outcome is predicted.
EFFECT: high accuracy of diagnosis.
FIELD: medicine, biochemistry.
SUBSTANCE: at testing one should precipitate high-molecular compounds with acetonitrile and register supernatant's spectral characteristics. Supernatant should be applied onto a paper filter, dried and put into solution containing aromatic aldehyde, acetone and concentrated hydrochloric acid taken at weight ratio of 70:5:1 to be kept for 2-3 min. Then it should be once again dried up to detect qualitative and semiquantitative content of oxidized tryptophan metabolites by intensity and chromatic shades. Moreover, by chromatic shades of yellow dyeing it is possible to detect the content of hydroxylated metabolites and by chromatic shades of violet dyeing - that of unhydroxylated ones.
EFFECT: higher significance of detection.
FIELD: medicine, anesthesiology, resuscitation.
SUBSTANCE: in patients one should study the content of lactoferrin in peritoneal exudates during the 1st d of postoperational period and at decreased value being below 3500 ng/ml on should predict unfavorable result. The suggested method provides correction of possible postoperational complications that deteriorate the flow of peritonitis and lead to lethal result.
EFFECT: higher accuracy of prediction.
FIELD: veterinary medicine.
SUBSTANCE: method involves determining low and middle molecular mass substances content in blood plasma and erythrocytes and general blood plasma albumin concentration. Integral index is calculated on basis of obtained values using formula II=100*S238-298(plasma)/S238-298(erythrocytes)*GAC, where S238-298(plasma) and S238-298(erythrocytes) are the low and middle molecular mass substances content in blood plasma and erythrocytes, respectively, determined from area of figures restricted by spectral curves in wavelength range of 238-298 nm and abscissa axis (conditional units2); GAC is the general blood plasma albumin concentration (g/l). The value being from 2.1 to 3.0, the first endotoxicosis degree is diagnosed. The value being from 3.1 to 4.5, the second endotoxicosis degree is diagnosed. The value being from 4.5 to 6.0, the third endotoxicosis degree is diagnosed. The value being greater than 6.0, the fourth endotoxicosis degree is diagnosed. The normal value is equal to 0.5-2.0.
EFFECT: high accuracy of diagnosis.
1 dwg, 1 tbl
SUBSTANCE: method involves separating blood serum proteins into fractions, determining albumins and alpha-2-globulins content and controlling their content changes during the disease development process. Gamma-globulin content is determined in per cent ratio with respect to total protein quantity. Then, changes in the fractions content are controlled from the first to the third week. Albumin content being in norm and alpha-2-globulins content becoming greater to the end of the first week by 30-50% when compared to normal value and dropping to norm at the second week end and gamma-globulin content increasing from norm by 10-30% to the second or the third week, high inflammatory process activity is to be diagnosed. Albumin content dropping by 10-30% from normal value at the second week, alpha-2-globulins content growing by 10-20% of norm and gamma-globulin content dropping by 30-50% at the second or the third week when compared to norm, low inflammatory process activity is to be diagnosed.
EFFECT: high accuracy and reliability of diagnosis.