Method of treating respiratory diseases in calves

FIELD: medicine.

SUBSTANCE: invention relates to veterinary science. The method involves a hypodermic injection of a hyperimmune serum containing specific PG-3, IRT and VD-MD virus antibodies every 24 hours to clinical recovery. It is combined with intake of 70% alcoholate of grinded herbal mixture containing equal proportions of purple echinacea herb and blossom clusters, Syrian rue herb, tillet blossom and common licorice roots. The mixture is prepared in the raw material to extractant relation 1:10, kept at temperature 18-20°C for 7 days. It is presented as 7-8% aqueous solution to be introduced in animals in dosage 2.5-3.5 ml/kg of live weight every 12 hours to clinical recovery.

EFFECT: method provides correction of the key broken components of immune system and enhancement of the natural resistance.

4 tbl, 2 ex

 

The invention relates to the field of veterinary medicine and can be used to treat respiratory disease in cattle caused by the Association of parainfluenza virus-3 (PG-3), infectious bovine rhinotracheitis (RTIs) and viral diarrhea is a disease of the mucous membranes (BD-BS) cattle.

Respiratory diseases of viral etiology widely distributed among cattle, and mass outbreaks are often caused by infectious bovine rhinotracheitis virus - pustular vulvovaginitis (RTI/IPV). Outbreak of RTIs in disadvantaged households are often accompanied by circulation among animals other pathogens, in particular of parainfluenza virus-3 (PG-3) and virus diarrhoea is a disease of the mucous membranes (BD-BS).

The usual treatment of animals affected RTI or mixed infection IRT-PG-3-VD-BS includes the use of symptomatic agents, immunomodulators and antibiotics to prevent secondary infections. The disadvantages of this treatment include a relatively low efficiency, because the pathogen persists in the animal population and after the termination of the flash, resulting in further repeated epizootics when sensitive population (reduction of the level of group immunity after a certain time after parabolani, the introduction of new Neiman the animal's etc). The lack of clear differences in nonspecific symptoms, viral, bacterial, and viral and bacterial respiratory diseases, as well as the impossibility of rapid diagnosis of their etiology leads to excessive use of antibiotics /1/. Experiments on the use of antibiotics for prophylaxis and treatment of calves showed that their appointment from the first days of life resulted in an increased incidence of bacterial complications.

Known methods of prevention and treatment of respiratory diseases using medicinal plants containing substances with a direct effect on the mucous membrane of the respiratory tract, such as leaves mother and stepmother, plantain, fruit, anise, fennel, caraway, herb thermopsis, oregano, thyme and other /2/in the form of infusions and decoctions, fees, including, for example, nettle, burdock, chamomile pharmacy, oregano ordinary, St. John's wort, common wormwood, plantain, mother-and-stepmother, oats /3/Ivan-tea, yarrow, plantain, mother-and-stepmother, burdock root, Polygonum pochechuynogo, Robotnik, dandelion, nettle, camomile /4/, leaves mother and stepmother, leaf of plantain shoots of wild rosemary, chamomile flowers, licorice root, leaf birch, rhizome with the roots of leuzea or grass Campion Tatar /5/, marsh rosemary (herb), mountains of the t bird (grass), elecampane (root), willow-herb (the leaves), small-leaved lime (flowers), the mother-and-stepmother ordinary (leaves), lungwort drug (herb), licorice (root), ordinary pine (kidney), thyme (herb), a series of tripartite (grass), greater celandine (grass), Althaea officinalis (roots), birch (kidney), black elderberry (flower), Valerian officinalis (rhizome), calendula officinalis (flowers), white mistletoe (sheet), sudanica swamp (grass), Pansy (flowers) /6/ and other Common disadvantage of known methods is not high enough efficiency due to the preservation of immunodeficiency calves, as it is known that respiratory diseases calves are accompanied by secondary T-immunodeficiency state, aggravating the disease process, and reducing the effectiveness of therapeutic and preventive effect.

The immune status of calves with respiratory disease increase passive immunization using immune sera of animal blood containing antibodies to pathogens that form the viral-bacterial background livestock complex, or immune serum donor animals, such as hyperimmune serum containing hemagglutinin to viruses RTI, PG-3 and BM-BS /7/.

The technical result - increase of efficiency of treatment of respiratory b the diseases of calves, which type of mixed infections, resulting in the formation of General nonspecific adaptive reactions of increased activation with increased resistance and balance different types of metabolism, on the background of passive immunization against viruses PG-3, RTI and VD-BS.

Getting the specified result is achieved by using an alcohol tincture of the chopped vegetable mixture of equal amounts of herbs and blossoms of Echinacea purpurea herb of Rue ordinary, Linden flowers (Tilia cordata) and the roots of the licorice, which is poured ethyl alcohol 70% concentration at a ratio of 1:10, incubated in the dark at a temperature of 15-20°C for 7 days, filtered, pressing raw and filtered.

Used ingredients are widely used in herbal medicine. The proposed collection contains all the necessary substances, the combined use of which has an effective influence on the animal organism in respiratory diseases.

Declared the collection has a bronchodilatory effect, promotes discharge of phlegm, anti-inflammatory and soothing effect, exhibits antiviral and antimicrobial activity, helps to restore the functions of the pulmonary system, has detoxifying properties, regulates metabolic processes and increases the body's resistance.

Grass and inflorescences of Echinacea purpurea possess immunomodulatory activity, inhibit the activity of hyaluronidase, the enzyme that enhances the permeability for viruses and microbes, increase the antimicrobial activity of leukocytes, macrophages and neutrophils in the blood and increase the synthesis of protective antibodies and endogenous interferon.

Herb Rue ordinary has a pronounced antimicrobial activity, confirmed pharmacological research, provides bronchodilatory effect collection, promotes the discharge of mucus.

Linden flowers Mescalito have immunomodulatory effects, which manifest themselves in action on the cellular and humoral mechanisms of immunity, the migration of lymphocytes, the synthesis of interferon and complement system, and anti-inflammatory action.

The roots and rhizomes of licorice provide bronchodilators and anti-inflammatory effect collection. In addition, they enhance the healing effects of other herbs included in the collection.

The chemical composition of the treatment-and-prophylactic means is represented by a complex of biologically active substances, which ensures its high therapeutic effect. The predominant biologically active substances are phenolic compounds, flavonoids, polysaccharides, alkaloids, tannins, VI the amines, trace elements, the main of which can be considered as zinc, selenium and iron.

Flavonoids (contain all components) inhibit the enzyme catechol-ortho-methyltransferase and provide adaptation trophic function of the body due to adrenoblokirutego effect. Flavonoids have P-vitamin (capillary) activity. In total anti-inflammatory effect of flavonoids is their direct effect on cell and tissue membranes - reducing their permeability, sealing membranes of lysosomes, inhibits hyaluronidase, an antagonistic effect against the inflammatory mediators.

Polysaccharides contain all components) have anti-inflammatory, immunocorrigirutee action. They stimulate nonspecific immunity and phagocytosis activity of macrophages and neutrophils, as well as bactericidal function of cells.

Phenolic compounds (contain all components) have an antimicrobial effect, the mechanism of which is due to their ability to absorb components of the cytoplasmic membrane of bacteria to form strong hydrogen bonds with proteins and damage the membrane, in particular increasing its permeability to ions (especially potassium) and important metabolites, lost cell, as well as for the water coming from the outside and promotes lysis.

78% of the total number of alkaloids contained in the herb Rue ordinary, is paganin. Pharmacological studies showed that 1%and 10%grass infusions of Rue ordinary have high protivostoyaschej activity, and a 0.25%solution of Paganini - high antimicrobial activity.

Tannins (Glycyrrhiza glabra - to 9.46%, small-leaved lime is not less than 8.0%), showing a stabilizing effect, restore repair processes in hepatocytes and improve the functioning of the antioxidant system of the liver.

Vitamins (vitamin C - Linden flowers (Tilia cordata), Echinacea vitamin E - licorice, vitamin a, Echinacea purpurea) are involved in all processes of metabolism, increase the body's resistance to diseases, anti-inflammatory action, promote detoxification.

The trace element zinc (small-leaved lime - 29 µg/g, licorice is 0.33 µg/g, Rue ordinary - 0.78 µg/g) participates in the body's immune processes, selenium (Glycyrrhiza glabra - 12,14 µg/g, Rue ordinary - 1.0 microgram/g), small-leaved lime to 0.13 µg/g) is a powerful antioxidant and iron (Echinacea - 48 mg/100 g, Linden (Tilia cordata) - 0.2 mg/g, Rue ordinary - 0.14 mg/g, licorice - 0.7 mg/g) the main structural component of hemoglobin and hem-comprising the farm is tov.

High collection efficiency is achieved by combining a complex of biologically active substances, as a result there is a mutual enhancement of the useful pharmacological properties of each individual component. Thus, the combination of vitamins a, C and zinc stimulates synthesis of antibodies, a combination of flavonoids and tannins provides a hepatoprotective effect, and the combination of flavonoids and fenologlikozida enhances the detoxification function of the liver. The collection has a pronounced anti-inflammatory, antimicrobial, antiviral, bronchodilators and adaptogenic action.

Tincture of the chopped vegetable mixture of grass and blossoms of Echinacea purpurea herb of Rue ordinary, Linden flowers (Tilia cordata) and the roots of the licorice preparing pharmacopoeial method in the ratio of raw material to izvlekali 1:10. As sulcatas use ethyl alcohol 70% concentration. Medicinal raw material is crushed to a particle size of 1-3 mm, mixed in equal quantities, a portion of the raw material is placed in a glass vessel, add the calculated amount of ethanol 70% concentration, incubated for 7 days in a dark place at a temperature of 18-20°C. Then alcohol tincture filter through a canvas or two layers of cheesecloth, pressing raw filter.

Example: in the glass vessel was placed 500 g of the medicinal si the article (an equal number from the grass and blossoms of purple coneflower, herb Rue ordinary, Linden flowers (Tilia cordata) and licorice root), added in 5.0 kg of ethyl alcohol 70% concentration, put in a dark place and kept at the temperature of 18-20°C for 7 days, after which it was filtered through the canvas, pressing the raw material, and was filtered.

A method of treating respiratory disease of calves is as follows.

Sick calves injected subcutaneously hyperimmune serum containing specific antibodies to the viruses, PG-3, RTI and VD-BS in the credits not less than 1:1280 and 1:256 1:1024, respectively, at a dose of 1.0 ml/kg body weight of the animal with an interval of 24 hours until clinical recovery and additionally applied inside 70% alcohol tincture of the chopped vegetable mixture of equal amounts of herbs and blossoms of Echinacea purpurea herb of Rue ordinary, Linden flowers (Tilia cordata) and the roots of the licorice in the form of 7-8% aqueous solution prepared in cooled boiled water a dose of 2.5-3.5 ml /kg body weight of the animal with an interval of 12 hours before clinical recovery.

The effectiveness of treatment for respiratory disease of calves polyetiological nature was determined in the research-and-production experiments in terms of the economy, long prone to respiratory diseases due to exposure to associations of viruses, pathogenic and conditionally pathogenic is Oh microflora, on the background of violations of conditions and feeding pregnant cows and calves. Virological and bacteriological studies confirmed etiologic role of PG-3 IRT antibody titer ranged from 1:4 to 1:32, pasteurellosis, Salmonella, Proteus and Streptococcus. The incidence of up to 80%, the mortality is up to 20%. The treatment was carried out at the first appearance of clinical signs, which were manifested by depression, decreased appetite, and in some cases the temperature increase to 41.5°C, pulse frequency up to 139 beats in 1 min and respiration up to 80 breaths 1 min, hyperemia of the mucous membranes covering the nasal speculum, serous-mucous effusions of the nasal holes and eyes. The forced killed calves during acute flow in the lungs was found dense regions of red color, signs of enfisema, mucopurulent exudate in the trachea and bronchi, the signs of catarrhal hemorrhagic gastroenteritis. Zapotocny and bronchial nodes are enlarged.

The essence of the method is illustrated by examples.

Example 1. Therapeutic efficacy of the method depending on its parameters were determined on the sick calves 30-45 days of age associated with a viral infection caused by viruses PG, RTI and VD-BS, after diagnosis on the basis of a comprehensive analysis required virological research is the Finance, distributed in 5 groups: 4 groups - experienced group 1 - control. Calves in all groups were subcutaneously injected with hyperimmune serum containing antibodies to viruses PG-3, ITR and VD-BS in the credits not less than 1:1280, 1:256 1:1024, respectively, at a dose of 1.0 ml/kg body weight of the animal with an interval of 24 hours before clinical recovery. Calves experimental groups additionally inside used 70% alcohol tincture of the chopped vegetable mixture of equal amounts of herbs and blossoms of Echinacea purpurea herb of Rue ordinary, Linden flowers (Tilia cordata) and the roots of the licorice in the form of a 7% aqueous solution with an interval of 12 hours in doses of 2.5, 3.0mm, 3.5mm and 4.0 ml/kg body weight of the animal to clinical recovery. Calves of the control group were treated by traditional methods with symptomatic means. Analysis was performed according to the main clinical characteristics (table 1). The results of the research (table 1) confirmed the validity of therapeutic doses of 2.5 - 3.5 ml/kg body weight of the animal. Doses below the minimum and above the maximum is not economically feasible.

Table 1
The effectiveness of treatment of respiratory diseases of calves depending on the parameters of the method
IndicatorsThe treatment
subcutaneously hyperimmune serum* at a dose of 1.0 ml/kg body weight every 24 hours until clinical recovery
inside tincture mixture of equal amounts of herbs and blossoms of Echinacea purpurea herb of Rue ordinary, Linden flowers (Tilia cordata) and the roots of the licorice with an interval of 12 hours at a dose ml/kg body weightSymptomatic remedies
2,53,03,54,0
The number of calves that goal.1 - n=182 - n=183 - n=154 - n=15n-20
Recovered a goal.1718151518
Form parabolani, %
light 1617141315
heavy21123
The duration of illness, days1210101016
Efficiency, %94,4100,0100,0100,090,0
Palo or involuntarily killed a goal.1---2
Recurrent disease, a goal.443010
* hyperimmune serum contains antibodies to the viruses, PG-3, RTI and VD-BS in the credits not less than 1:1280,1:256 1:1024, respectively

Example 2. The treatment efficiency of the JV is the property in accordance with the invention in comparison with known was determined on 2 groups of patients calves 40-45 days old 10 goals each. Calves had typical clinical signs of infectious respiratory disease (depression - calf lowers ears, is some distance away from the main group of animals; decreased appetite, increased body temperature to 40.8°C). The diagnosis was established according to the results of the anamnesis, clinical, virological and bacteriological studies. Calves from the experimental group were treated with subcutaneous injection of hyperimmune serum containing specific antibodies to the viruses, PG-3, RTI and VD-BS in the credits not less than 1:1280, 1:256 1:1024, respectively, at a dose of 1.0 ml/kg weight of the animal with an interval of 24 hours and an additional oral use 70% alcohol tincture of the chopped vegetable mixture of equal amounts of herbs and blossoms of Echinacea purpurea herb of Rue ordinary, Linden flowers (Tilia cordata) and the roots of the licorice in the form of a 8% aqueous solution at a dose of 3.5 ml/kg body weight of the animal with an interval of 12 hours until clinical recovery. Calves of the control group were treated with symptomatic drugs, including anti-bacterial, and serum of animals, hyperimmunizing vaccine PG-3 and RTI "CAMP". The animals were clinically observed for months. Studies of clinical and physiological parameters was carried out during the week. Before and after the treatment took blood samples for opredelyayushchikh indicators. The results are presented in tables 2, 3, 4.

Table 2
Clinical and physiological parameters calves
Day treatmentThe control groupExperienced group
body temperature, °Cpulse beats./minbreathing rate, movement./minbody temperature, °Cpulse beats./minbreathing rate, movement./min
Day 140,8±0,2to 96.8±1,5324,6±1,0340,9±0,295,4±2,2025,0±1,48
Day 240,4±0,3104,0±3,0328,6±2,2541,0±0,3103,0±4,0328,8±1,16
Day 3 39,7±0,494,0±1,8724,8±1,1B39,5±0,298,0±2,0224,8±1,16
Day 439,2±0,4to 96.6±2,1622,6±1,8738,6±0,395,2±1,9820,8±1,97
Day 539,1±0,3of 92.2±2,1018,8±2,1638,9±0,2of 92.6±1,4718,8±1,39
6-Eugene38,3±0,1of 92.8±2,0221,8±2,8038,2±0,1the 95.8±1,7821,8±1,28
Day 738,9±0,2is 95.6±1,5720,2±2,6638,1±0,2to 90.4±1,4424,2±1,28

Table 3
The effectiveness of treatment of respiratory bol is SNA calves
IndicatorsThe treatment
subcutaneously hyperimmune serum and inside the infusion of a mixture of equal amounts of herbs and blossoms of Echinacea purpurea herb of Rue ordinary, Linden flowers (Tilia cordata) and the roots of licorice, n=10medications and blood serum of animals, hyperimmunizing vaccine PG-3 and RTI, n=10
Recovered a goal.108
The duration of illness, days812
Efficiency, %100,080,0
Palo or involuntarily killed a goal.-2
Recurrent disease, goal34

Table 4
Immunological parameters of blood in patients with calves
IndicatorsSick calves
Before the treatmentAfter the treatment
Experienced groupThe control group
Leukocytes, 109/l14,54±0,6213,04±1,2813,87±0,95
Lymphocytes, 109/l7,33±0,318,27±0,628,71±0,44
T-lymphocytes, 109/l19,8±1,623,7±1,120,0±1,4
Total protein serum, g/l68,9±3,675,0±3,472,6±3,5
Globulins, %
alpha
beta
gamma
58,06±12,14
10,70±4,37
rate 18.89±2,12
28,47±3,76
60,26±11,12
9,39±1,87
16,07±0,97
34,78±6,77
52,86±12,11
br15.15±0,03
15,11±0,98
22,6±4,91
Albumin, %was 41.94±13,7839,16±9,3547,14±3,99
Bactericidal activity of blood serum, %28,3±1,1 50,0±1,939,9±2,0
The authors estimated the effect activity of blood serum, %1,8±0,44,6±0,23,2±0,2

The analysis in table 2 showed that the body temperature of patients calves in the first few days of treatment was higher than the physiological norm. Normalization temperature in calves from the experimental group occurred on the third day, and in the control group - 4-5 day of illness. Heart rate and respiration during the first days of illness were at the upper end or above the physiological norm, for 5-6 day treatment normalization of these parameters as the control calves and calves from the experimental group. The symptoms of the disease in calves of the experimental group was gone for 8-10 days in the month (period), not re-occurred. Calves normally developed. The clinical manifestations of respiratory disease in calves in the control group amounted to 10-12 days, 4 calves from 8 recovered developed recurrence (table 3). When treating sick calves along with the disappearance of symptoms characteristic of a marked increase in the number of immune cells. Thus, the number of T-lymphocytes in the experimental group increased by 19.7%, while in the control group, this rate was almost unchanged. Total protein was increased in the experimental Ruslana's group of 8.9% compared to 5.4% in the control group, moreover, in the control group, this increase was due to albumin and alpha-globulin fraction, while in the experimental group is due to the gamma-globulin fraction. It can be assumed that higher levels of proteins alpha-globulin fraction due to the ongoing inflammatory process, because the alpha globulins include proteins "acute phase". The increasing levels of gamma-globulin indicated to increase the voltage of the immune system, as the number of gamma globulins are immunoglobulins of classes A, G and M, with the properties of antibodies (table 4).

The tests confirmed the high efficiency of the method of treatment of respiratory disease in calves.

Sources of information

1. Danilevsky V.M. Internal non-infectious diseases of farm animals. - M.: Agropromizdat, 1991. - P.148.

2. Dccallow and other Drugs in veterinary medicine. - M.: Kolos, 1977, S.138-147.

3. Veterinary medicine, 2005, №4, C.11-13.

4. EN 2072862 C1, 1997.

5. EN 2184560 C1, 2002.

6. EN 2185184 C1, 2002.

7. EN 2287994 C1, 2006.

A method of treating respiratory disease of calves, including subcutaneous injection of hyperimmune serum containing specific antibodies to the viruses, PG-3, RTI and VD-BS, with an interval of 24 h until clinical recovery, characterized in that it further inside apply 70%alcohol tincture of the ISM is Lannoy herbal mixture of equal amounts of herbs and blossoms of purple coneflower, herb Rue ordinary, Linden flowers (Tilia cordata) and the roots of the licorice obtained when the ratio of raw material to izvlekali 1:10 and aged at a temperature of 18-20°C for 7 days, in the form of 7-8%aqueous solution, at a dose of 2.5-3.5 ml /kg body weight of the animal with an interval of 12 h prior to clinical recovery.



 

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26 cl, 40 dwg, 3 tbl, 38 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: present invention refers to application in an effective amount and to new nicotine receptor agonists described by general formula (i) or (ii) for treating inflammatory diseases chosen from a group including asthma, chronic obstructive pulmonary disease (COPD), interstitial pulmonary tissue fibrosis (IPF), sarcoidosis, hypersensitivity pneumonitis (HP), chronic hypersensitivity pneumonitis and bronchiolitis obliterans organising pneumonia (BOOP). The compounds (i) and compounds (ii) relate to formulae (i) (ii) where in formula (i) R1 and R2 independently mean alkyl with 1-10 carbon atoms; Xa means CH or N; Ya means one or more substitutes chosen from hydrogen, halogen, cyano, hydroxyl, alkyl with 1-10 carbon atoms optionally substituted with one or more halogen atoms, and alkoxy with 1-10 carbon atoms; n means an integer 0 or 2; J means a counterion representing a compound for maintaining electric neutrality, e.g., halogen, sulphate, sulphonate; in formula (ii) R3 is chosen from or Xb means N or N+-R10; R4 means one or more substitutes chosen from hydrogen, halogen; each R10, R11 and R12 independently means alkyl with 1-10 carbon atoms; provided the presence of the counterion when Xb means N+-R10.

EFFECT: use of nicotine receptor agonists in the effective amount for treating inflammatory diseases.

26 cl, 40 dwg, 3 tbl, 38 ex

FIELD: medicine, veterinary medicine.

SUBSTANCE: invention relates to the field of veterinary medicine and biotechnology. The method includes selection of donors, selection of donor blood, holding at room temperature for clotting, separation of blood clot, serum treatment, placing in the refrigerator, checking on harmlessness and activity, packaging into sterile containers. At that a maternal and a closely related group of cattle are used as donors, who previously suffered from viral respiratory-intestinal infections, and the serum is pre-examined for antibodies presence to previously registered pneumoenteritis of viral genesis by raising the reaction of indirect hemagglutination. The blood is then incubated at room temperature for 1 hour and placed in a refrigerator at a temperature of 4-6°C for 12-16 hours for complete retraction of blood clot. After checking of serum on harmlessness and activity the tanks are treated outside with 1% solution of "Zhavel-solid", uncorked in a sterile room, filtered, centrifuged and poured into sterile containers. Then the serum is irradiated with ultraviolet rays with a length of 254-256 nm during 1 hour and ozonised in a closed loop mode.

EFFECT: method does not use toxic substances, increases accuracy of determining activity of serum and accelerates duration of its preparation, and serum obtained with this method has a high specificity and sterility.

FIELD: chemistry; biochemistry.

SUBSTANCE: invention relates to biotechnology, specifically to obtaining a human immunoglobulin based preparation, and can be used in medicine. The preparation is obtained via purification of class G, A and M immunoglobulins isolated from the blood of HIV infected patients through affinity chromatography on a column with integrase-sepharose.

EFFECT: invention enables to obtain class G, A and M immunoglobulins isolated from the blood of HIV infected patients, capable of selectively splitting HIV integrase only.

7 dwg, 4 ex

FIELD: chemistry.

SUBSTANCE: invention pertains to biotechnology. Described is an anti-HIV monoclonal antibody or its functional fragment which has in the variable region of the H-(heavy) chain regions which determine complementarity, CDR1, CDR2 and CDR3, which have sequences given in the description, and CDR1, CDR2 and CDR3 regions with sequences given in the description in the variable region of the L-(light) chain. Expression vectors which code fragments of the heavy and light chain of the described antibody and host cells which are transformed by the said vectors are disclosed. A method for detecting the HIV strain and a method for individual passive immunotherapy are disclosed.

EFFECT: invention enables to obtain an antibody which enables neutralisation of HIV infection without an autoimmune side effect.

17 cl, 9 dwg, 2 tbl, 6 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine and concerns a composition for treatment or prevention of infection and/or infectious disease of respiratory tracts and the method for application of said composition for specified purposes which involves oral introduction to a mammal of the composition containing a galactose-containing indigestible oligosaccharide and at least 5 wt % of digested galactose saccharide.

EFFECT: invention provides that the active substance is safe and can be included in the dietary intake.

20 cl, 7 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention presents the method and composition for treatment and-or prevention of infection, and said method includes oral introduction of the composition to a mammal, and said composition involves galactose-containing indigestible oligosaccharide and immunoglobulin of milk or colostrum of hyperimmunised cows.

EFFECT: development of the effective method for the compositions for treatment and-or prevention of infection.

15 cl, 1 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: method is suggested for production of virus on lung cells of cotton rats, and also line of lung cells of cotton rats ATCC PTA-3930 for growth, reproduction and cultivation of viruses.

EFFECT: improved efficiency of method.

8 cl, 1 tbl, 7 ex

FIELD: medicine.

SUBSTANCE: invention concerns medicine, particularly therapy, and can be applied in chronic disease treatment. Method involves causative agent extraction or determination of antibodies to causative agents or extraction of genetic components from blood, body fluid or smear. Treatment is performed by vaccine drugs specific to one or several identified causative agents, additionally immunomodulators are administered.

EFFECT: activated repair processes, arrested development of disease state of organs and systems due to elimination of identified pathogen from organism and to immune correction.

3 cl, 8 ex

FIELD: biotechnology, preparative biochemistry.

SUBSTANCE: method involves hyperimmunization of animal-producers with inactivated, purified and concentrated rabies virus with aluminum phosphate and/or sodium nucleinate as an adjuvant. Virus obtained in culture transplantable cells VERO and in primary culture of Syrian hamster kidney cells is concentrated by adsorption of aluminum phosphate gel or centrifugation, or ultrafiltration methods and purified passing through porous silica, or by ion-exchange chromatography. For immunization antigen with the immunogenicity index 9.5 IU/ml, not less, is used and with the content of protein less 200 mcg/ml, bovine serum albumin less 0.5 mcg/ml Syrian hamster kidney cells and VERO cells less 0.5 mcg/ml, cellular DNA 5.0-10.0 ng/ml, aluminum ions concentration in antigen 0.2-1.25 mcg/ml, and sodium nucleinate 0.002-0.5 mg/ml. Scheme of grund-immunization - 1-3 injections of antigen in the dose 3-15 ml with interval for 14-30 days, the main immunization cycle - on 45-60 day involving 10-15 injections with interval for 5-15 days and in the antigen dose 5-70 ml. Then producers are immunized once per a month with the dose 20-70 ml, and once per 6 month the shortened cycle of immunization is carried out involving 2-4 injections of antigen with interval for 5-15 days in the dose 20-70 ml. Method provides preparing heterologous antirabies serum of high specific activity.

EFFECT: improved preparing method, improved and valuable properties of serum.

12 cl, 1 tbl, 4 ex

FIELD: biotechnology, protein engineering.

SUBSTANCE: claimed library represents E.coli TGI cells wherein each cell contains fragmid DNA providing biosynthesis of filamentous bacteriophages exposing unique human single-stranded antibody on surface thereof. Also disclosed is recombinant fragmid pHEN-2A8 DNA containing artificial gene of human single-stranded antibody under control of lactose operon promoter providing synthesis of human single-stranded antibody in composition of chimerical protein with membrane pIII protein of M13 bacteriophage in E.coli cells. Also disclosed is method for production of artificial human single-stranded 2A8 antibody by using such fragmid DNA.

EFFECT: fragmid library useful in medicine.

3 cl, 7 dwg, 10 ex

FIELD: veterinary medicine.

SUBSTANCE: means has alcohol extract of herb and inflorescences of Echinacea purpurea, Tussilago farfara, Thymus serpyllum herb and Glycyrrhiza glabra roots taken in equal quantities. Method involves introducing intramuscular recovalescent serum injection containing specific antibodies to coronary rotaviruses in 1:128 and 1:64 proportions respectively. Alcohol extract of herb and inflorescences of Echinacea purpurea, Tussilago farfara, Thymus serpyllum herb and Glycyrrhiza glabra roots taken in equal quantities as 7-8% water solution is additionally administered for 7-10 days with 24 h long intervals at a dose of 1.0-1.5 ml/kg of animal weight as prophylaxis and at a dose of 2.5-3.0 ml/kg as treatment with 12 h long intervals until clinical recovery takes place.

EFFECT: simplified and high accuracy diagnosis method.

2 cl, 4 tbl

Anti-decubitus oil // 2416425

FIELD: medicine.

SUBSTANCE: invention refers to creation of fatty oil and essence compositions for decubitus ulcer treatment. The anti-decubitus oil contains sea-buckthorn, wheat-germ fatty oils, Alexanrdia laurel oil and essences: origanum, geranium, cedar, marjoram, myrrh, palmarose, patchouli, guaiacum, tanacetum and tea tree in the certain proportions.

EFFECT: provided maximum circulation management within an injury, rejection of necrotic mass (the same decubitus ulcer), skin restoration within the decubitus ulcer and the fastest cleansed wound repair.

3 ex

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