Method for prediction of cerebral affections in newborns
SUBSTANCE: five-percent placental tissue extract is analysed for the concentration of arginine and histidine to be related, and if the relation is lower than 0.86, development of cerebral affection in a newborn is predicted.
EFFECT: more precise and specific prediction of perinatal CNS affection in the newborn and enabled well-timed pathogenetic therapy.
3 dwg, 1 tbl
The invention relates to medicine, namely to obstetrics and Perinatology, and can be used to predict lesions of the Central nervous system of the newborn.
Perinatal brain damage takes the leading place in the structure of morbidity and mortality, due to a progressive increase in the frequency of cerebral disorders in newborns, leading to their role in the formation of a disability since childhood. In this regard, this problem is beyond the scope of biomedical and acquires social value (Beresnev, Y.M. Hypoxic encephalopathy: the hypothesis of the pathogenesis of cerebral disorders and the search for methods of drug therapy //Ross. Bulletin of perinat. and pediat. 2002. No. 1. - P.6-13).
Prenatal factors leading to the defeat of the brain of the newborn can be different: diseases transferred by the woman during pregnancy, perinatal infection, immunological imbalance, metabolic disorders in the mother's body. However, the leading role among them plays intrauterine hypoxia that accompanies many of the complications of pregnancy. Despite these successes remain some difficulties in accurate and objective diagnosis and especially the prediction of perinatal brain damage.
Thus, there is a need develo the TCI of new methods for the prediction of cerebral damage the newborn with the purpose of timely adequate pathogenetic therapy, to reduce the frequency of manifestation and severity of cerebral pathology.
Among well-known in the medical-scientific and patent literature methods of diagnosis of brain damage can be noted electroencephalography of the fetus at birth, but the accuracy of this diagnosis is very low due to functional immaturity of brain structures and the large variability of the bioelectrical activity of the brain of the fetus (Beresnev SCI, Bubnov NI, Sorokina SH and other Perinatal brain pathology: the margin of safety, and short and long-term prognosis // ROS. news. perinatol. and the pediatrician. - 1998. No. 4. - P.6-12). Data on the diagnostic value of determining the level of α-fetoprotein in the amniotic fluid for the specified purpose relate mainly malformations (neural tube Whirlow et al. Amniotic fluid. - 1998. - S). Fairly accurate, however, very traumatic, is a method of determining neurospecific peptide - homocarnosine in the amniotic fluid taken during pregnancy by amniocentesis (Madhu M Homocarnosine amniotic fluid and its value in the diagnosis of brain damage in the fetus. - Abstract. Diss. Kida. the honey. Sciences. 1990. - 19 C.). Traumatic is the diagnosis and perinatal CNS damage by determining the intensity of processes of lipid peroxidation in the Kyrgyz Republic the wee baby at 3 and 6 days of life (Lagumina NN. Early diagnosis and preventive therapy of perinatal CNS lesions in children. Rostov-on-don. - Abstract. Diss. Kida. the honey. Sciences. 1998. - 22 S.).
There is a method of predicting health disorders children with perinatal CNS damage in the first year of life on the study of umbilical cord blood, wherein determine the relative content teilinstitut cells in their content of 9% and over predict the preservation of neurological symptoms by the end of the first year of life (patent RF №2121682, 1998). The disadvantages of this method include nespecificnomu - change T-link the immune system takes place and other pathological conditions.
In the patent of Russian Federation №2148262 (1999) proposed a method for the diagnosis of hypoxic lesions of the Central nervous system in newborns using the definition of the MDA content in the cerebrospinal fluid of a child in the 2nd and 6th day of life. With the increase of this indicator on the 2nd day over to 0.72 µmol/l and on the 6th day in 2 times in comparison with the norm diagnose the progression of the pathological process in the brain. This method is traumatic because it required taking liquor from a child in the neonatal period, which may lead to serious consequences.
There is a method of forecasting encephalopathy in newborns (RF patent No. 2157534, 1999), which in cord blood define an is iheni loci And, In, DR HLA system, and if one of the antigens A32, B8, B27, DR3 or combinations thereof predict perinatal hypoxic-ischemic encephalopathy. The method is tedious and not sufficiently specific - different loci HLA-system observed in various diseases, including systemic.
In the patent of Russian Federation №2254573 (2005) proposed a method for predicting newborn encephalopathy by determining the activity of NADPH-oxidase in placental homogenates. Increased activity of this enzyme to 13.5 units of optical density and above allows us to predict the development of encephalopathy in the newborn. The disadvantage of this method is its lack of specificity. Proposed in this method, the enzyme activity is determined for diagnostic purposes, firstly, does not reflect the state of the placental blood flow and hemodynamics, the violation of which is the primary cause of hypoxic-ischemic brain injury fetus and newborn. Secondly, disorders of energy metabolism that can lead to serious modification of biochemical processes in the brain, primarily associated with the reactions of oxidative phosphorylation and metabolism of makroergov, and then the ratio of NADPH and NADP (Lubchenco A.M., Lukyanova L.D. Trigger role of energy metabolism in the cascade of functional-metabolic the definition of violations during hypoxia. //Problems of hypoxia: molecular, physiological and medical aspects. - M. 2004. - P.51-84).
Another method for early diagnosis of perinatal CNS lesions in newborns is the concentration of MDA in the amniotic fluid (RF patent No. 2289136, 2005), and increased more 4,50 µmol/l diagnosed with perinatal CNS damage. However, this method is non-specific, because the increase in MDA level in the amniotic fluid can lead not only to the defeat of the Central nervous system, but also to cardiopathy newborn and thyroid gland (Vierow et al. Amniotic fluid.-1998. - 215 C.). Therefore, this method can only be used in screening tests.
Similar disadvantages are characteristic of the diagnostic method of the consequences of perinatal CNS damage is mild on the patent of Russian Federation №2312351 (2006), where the diagnostic test is proposed total level of antibodies in the serum of children. This indicator is also not specific in relation to cerebral damage and can, in particular, increase in autoimmune diseases in children (Menshchikova E.B., Zenkov NICHOLAS, Shergin S.M. Biochemistry of oxidative stress. Oxidants and antioxidants. Novosibirsk. - 1994. - 203 C.).
There is a method of diagnosing consequences of perinatal CNS lesions, mild (pathé is t No. 2339043, 2007) by estimating the level of IL-1β in the serum. In this way not predicted the development of CNS lesions in the newborn, and already diagnosed with the aftermath, and therefore cannot timely pathogenetic therapy before irreversible complications.
Thus, the main disadvantages of the above methods of prediction and diagnosis of cerebral disorders in newborns are: in some cases the trauma, in the other - nespecificnomu or complexity, in some cases the need to use expensive equipment to perform the analysis.
The prototype of the claimed invention, the selected method for predicting CNS pathology in newborns (patent No. 2317043, 2008).
The essence of the prototype method is that in cord blood of a newborn determine the level of endothelin, and on the sixth day spend dopplerometrical the study of the middle cerebral artery, and if the level of gli is 8,14 PCG/ml or more, the resistance index is equal to 0.73 and more, predict the development of neurological symptoms in this child in the first 3 months of life.
This method has several disadvantages:
1. The impossibility of predicting neurological disorders in the newborn immediately after birth (Doppler is only about 6 hours).
2. The method is not sufficiently specific - changes in the level of endothelin in the umbilical cord blood reflects only one component of a complex process of regulation of the tone of blood vessels. The effect of the vasoconstrictor may be reduced due to the opposite effect extremely active vasodilators and primarily of nitric oxide (Reutov V.P. Cycle of nitric oxide in mammals // Success Biol. chemistry. - 1995. - T.35. - P.189-228).
3. The method is time-consuming, its implementation requires expensive equipment and import sets of reagents for immunoassay analysis, which hinders its widespread use in clinical practice.
4. Some degree of inaccuracy in the results diplomaticheskii changes can make extraordinary lability vascular reactions of the newborn, and severe stages of flow hypoxic lesions of the Central nervous system (change vasospasm and vasodilation) in this period.
These drawbacks are proposed to be eliminated in the present method. The objective of the proposed method is to develop to enable specific way with high accuracy to predict cerebral damage in the newborn.
The problem is solved in the following way: after delivery of placenta (at the final stage of labor) take a portion of its tissue, prepare a 5%extract, which definition shall have the content of arginine and histidine, calculate the ratio of the first to the second, and when the magnitude of the relationship below 0,86 predict the development of cerebral lesions in infants.
The technical result is obtained during use of the invention is to improve the accuracy of predicting the development of cerebral lesions in the newborn, which allows you to assign in the early neonatal period adequate pathogenetic therapy.
The novelty of the proposed method lies in the fact that for the first time proposed to predict cerebral lesions in the newborn in the early neonatal period, and in the case of a delayed their development on a single definition in the placenta ratio of the content of arginine and histidine.
Biochemical and, as a consequence, the functional changes in the placenta (placental insufficiency) are the main trigger in the development of the violations of her hemodynamics and fetal-maternal circulation, accompanied by chronic intrauterine hypoxia (Savelyeva G.M. et al., Placental insufficiency M.: "Medicine". - 1991. - 276 C.). The latter, in turn, can lead to the development of cerebral damage the fetus and later a newborn baby.
It is now established that Autonomous control of placental blood flow is competing influence of the vases is active compounds - signal molecules produced locally in the placenta. These include nitric oxide (NO), which has an extremely wide spectrum of action. He is not only a potent vasodilator, but neurotransmiters (Snyder S.H. //Science. - 1992. - vol. 257. P.494-496), controls the aggregation of platelets to the endothelium, regulates oxygen transport processes (Markov oil on canvas //Advances in physiological Sciences. - 1996. - So 27. No. 4, P.30-43). However, NO is a short - lived molecule in different tissues exists from a fraction to several seconds, and its metabolites present an incomplete picture of the possible effects of nitric oxide. As you know, the main source of NO synthesis is the amino acid arginine (E.S. Severin, Biochemistry. M: GEOTAR-MED. 2003, S-188), the contents of which allows to judge about the dynamics NO. In addition, arginine is involved in the regulation of many physiological processes.
Arginine is used in the tissues as a building material and energy, as well as operates as an independent signaling molecule. He is involved in communication between nerve cells (Dmitrienko EXAMPLE, the gut THUS, Chandarana YEAR, Ukr. her oncologist. log. No. 1. - 2008. - P.137-140).
An essential amino acid synthesized in the placenta and also performs numerous functions, is histidine, imidazole ring which expands its participation in biochemical reacts is Yah. Education high energy derivatives of imidazole may affect tissue energy pool. The product of the decarboxylation of histidine - histamine exerts opposite nitric oxide vasoconstrictor effect, affecting the tone of the placental vessels and, consequently, the intensity of circulation. Simultaneous determination of placental amino acids with multidirectional influence on the blood vessels endothelial component allow you to more objectively judge the state of the placental blood flow and fetal as oxygen and plastic substances necessary for proper development of the fetal brain and then the brain of the newborn.
We installed a prognostic parameter for the development of perinatal brain damage, by definition, 5%placenta extract ratio arginine/histidine, when the value is below 0,86 predict cerebral lesions of the newborn.
The method is as follows.
Before conducting biochemical research areas of the Central part of the placenta is washed from the blood chilled saline solution, weighed 1 g of tissue and the extraction is carried out amino acid 10% solution of trichloroacetic acid (in the ratio 1:5). To precipitate proteins, the mixture is centrifuged at 3000 rpm for 10 minutes. The supernatant in an amount of 0.1 ml of UNOSAT on a strip of chromatographic paper (density 60-80), pre-moistened with acetate-pyridine buffer solution. Electrophoresis of amino acids is carried out in the same buffer at a pH of 5.2 to 5.4 in the instrument for electrophoresis (Institute of physiology. Bohomolets, Ukraine). The duration of electrophoresis for 2 hours at 120 V/cm and a current of 0.8 mA/see At the end of electrophoresis pregrammy fixed in a drying Cabinet at 90° C for 10 min and stained with 0.5% ninhydrin solution in acetone. The color intensity is measured on the spectrophotometer SF-26 (Russia) at a wavelength of 530 nm. The location of the arginine and histidine on ForageMax identify coincidentally mobility spots - the standard of the corresponding amino acids with mobility spots placenta extract. Calculations of the content of arginine and histidine carried out on the calibration graphs prepared using standard solutions of amino acids (ICN, Belgium).
The performance of the proposed method is confirmed by the following clinical examples.
Example 1. Pregnant I-VA, 25 years. East. No. 906. The patient was in the dispensary in the antenatal clinic with 10 weeks of pregnancy. The first pregnancy. Entered the maternity ward 7.10.2008, Diagnosis: pregnancy 39 weeks, fetoplacental insufficiency. In the second trimester was installed anemia, on this occasion, has taken the necessary treatment. The first period is on delivery. 8.10.2008, was born full-term baby, the baby's weight at birth 3200 g, height 51 cm, estimation on Apgar scale 8/8 points.
Study 5%placenta extract has allowed to establish that the contents of arginine and histidine 0.85, indicating that opportunities for the development of CNS pathology in the newborn. The child was discharged from the hospital on the 6th day in a satisfactory condition. However, at the age of 2 months when viewed by the child neurologist identified General motor restlessness, tremor of extremities, muscle dystonia, reflexes quickly fade away. The clinical picture was rated as perinatal hypoxic-ischemic lesion of the Central nervous system.
Thus, the prediction made by the present method, were confirmed.
Example 2. Pregnant C-WA, 29 years. East. No. 793. Was at the dispensary in the antenatal clinic with 9 weeks of pregnancy. This first pregnancy. Entered the maternity ward 4.08.2008, Diagnosis: pregnancy 39-40 weeks, late preeclampsia, placental insufficiency. The first stage of labor. 5.08.2008 occurred this term spontaneous labour. The weight of the baby at birth 3300 g, height 52 cm, estimation on Apgar scale 7/8 points. In the study of 5% placenta extract the contents of arginine and histidine amounted to 0.80, which allowed to predict the development of perinatal porazeni the Central nervous system. From the first days of life the newborn was noted anxiety, tremor of extremities, muscle hypertonicity, the weakening of the sucking recovery of reflexes. Diagnosed with hypoxic-ischemic CNS damage the child was transferred to the neonatal care unit.
Thus, the prediction made by the present method, were confirmed.
Example 3. Pregnant E, 28 years. East. No. 849. The first pregnancy. The patient was in the dispensary in the antenatal clinic with 9 weeks of pregnancy. Entered the maternity ward 24.09.2008 was diagnosed with pregnancy 39-40 weeks, early preeclampsia, pyelonephritis. 25.09.2008, was born full-term baby, the baby's weight at birth 3250 g, height 52 cm, estimation on Apgar scale 8/8 points. Condition at birth satisfactory. In the study of 5%placenta extract the value of the ratio of the content of arginine and histidine was 0.86, indicating that the absence of risk of the development of the Central nervous system in the newborn. The child was discharged on the 6th day in a satisfactory condition. Subsequent examinations neurologist in 2 months and 6 months revealed no neurological pathology.
Thus, the prediction by the present method were confirmed.
By the present method, we have examined 102 women with a 5%placenta extract the content of arginine and histidine and the calculated is an ratio of these amino acids. Of the surveyed mothers in 39 the ratio of arginine to histidine was below 0,86, and 63 is 0.86 and above. In 38 children, in the placenta of mothers which studied the figure below 0,86 found the CNS, and only one child in this group cerebral CNS lesions within 6 months of follow-up was lacking. All babies born to women with placental ratio of arginine to histidine 0,86 and above, had no neurological symptoms either at birth or in later periods of postnatal development (up to 6 months). These studies are presented in the table.
|Index||The number of the examined|
|False positive result||1|
From the presented data it follows that the accuracy and specificity is 99%.
The above confirms prognosticheskoe value of the claimed method.
Thus, the claimed method is more effective in comparison with the known and has a number of advantages:
1. High accuracy - 99%, specificity 99%.
3. Normally requires a single study.
4. The availability of way into widespread clinical practice.
5. The possibility of prediction of perinatal brain lesions of the newborn and timely preventive treatment of this pathology.
The inventive method for predicting cerebral damage in newborns allows to carry out preventive therapy to reduce the risk of irreversible complications and can be widely used in clinical practice.
A method for predicting cerebral lesions in newborns, including the study of biological material, characterized in that the five percent extract of placenta tissue determine the content of arginine and histidine, compute their value and when the value is less than 0,86 predict the development of the newborn cerebral lesions.
SUBSTANCE: patient's blood serum is treated with 7% solution of polyethylene glycol-6000, incubated and with dye Sudan B at 40°C for 1 hour, separated electrophoretically in agarose gel. After that, additionally, before treatment of blood serum with 7% PEG-6000 to 0.6 ml of sample 0.2 ml of 0.1% tritone X-100 solution is added, incubated for 15 minutes at 20°C, after which mixture is mixed by shaking 120 times per 1 minute. Application of method makes it possible to detect additional intensive minor fraction of modified LP(a).
EFFECT: increase of diagnostics accuracy.
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SUBSTANCE: method of determination of triterpene saponins in vegetable raw material and medications includes dissolution of saponin-containing fraction in mixture water-ammonium buffer, determination of its optic density and calculation of saponin content in terms of oleanolic acid, under specified conditions.
EFFECT: claimed method represents express method, facilitates analysis and increases degree of reliability of obtained results.
SUBSTANCE: in order to estimate efficiency of treating ischemic nephropathy in newborn babies in early neonatal period activity of gamma-glutamyltransferase and cholinesterase in child's urine is determined in dynamics of treatment. If activity of said enzymes decreases with respect to initial level, treatment is estimated as efficient, if activity increases or does not change - as inefficient.
EFFECT: application of method makes it possible to increase accuracy of estimation of ischemic nephropathy treatment in newborns, carry out correction of therapeutic measures in due time and improve disease outcome.
1 tbl, 3 ex
SUBSTANCE: in a first trimester of pregnancy, the microalbuminuria level is determined. If the value is 45 mg\l and more, placental insufficiency is predicted.
EFFECT: method enables early prediction of placental insufficiency by a simple quantitative estimation and thereby ensures early adequate preventive treatment.
SUBSTANCE: detecting expression of GalNac-T14 molecules enables to predict sensitivity or indicates that a tissue or cell sample is sensitive to apoptosis inducing agents, such as DR4 or DR5 agonist antibodies. The information obtained by the analysis aimed at detecting GalNac-T14 expression in the mammal's tissue or cell sample can provide a hospital doctor with data which can be used for prescribing an optimal treatment schedule for patients suffering such diseases as pancreas cancer, lymphoma, non-small cell carcinoma of lung, colon cancer, rectal cancer, melanoma or chondrosarcoma.
EFFECT: expanded scope of the compounds.
16 cl, 53 dwg, 14 tbl
SUBSTANCE: invention relates to field of medicine, in particular toxicology and resuscitation science and can be used for early prognosis of course of acute poisoning with psychotropic drugs. At the time of patient's admission to hospital albumin fraction of blood serum is isolated. After that, general level of reduced thiols is determined, and if its value is lower than 220 mcmol/l development of negative disease dynamics is predicted.
EFFECT: method allows to increase efficiency of performed treatment in said category of patients.
1 tbl, 8 ex
SUBSTANCE: invention refers to chlitin derivative photoproteins and to application thereof both as intracellular calcium indicators, and in cellular studies. Said proteins are produced by mutagenesis of a coding sequence of chlitin. Also, there are offered nucleic acids coding said protein, a vector containing said nucleic acids and a host cell carrying the vector. They can find application in genetic communication technologies for monitoring the cellular events associated with signal transmission and gene expression. Besides, photoproteins of the present invention can be used as intracellular calcium indicators in diagnostic techniques based on calcium concentration measurement in response to the various effects.
EFFECT: produced proteins exhibit enhanced bioluminescence, high affinity to calcium and prolonged light emission.
19 cl, 16 dwg, 5 ex
SUBSTANCE: in order to realise the method level of ferritin in blood serum and bile bladder tissue, rang point is defined, and ferritin values in serum from 0 to 10 ng/ml, and bile bladder from 0 to 0.25 mg/l - are taken for 1 point, respectively, level of ferritin in serum 70 ng/ml is taken as 7 points, and in bile bladder tissue 0.75 mg/l is taken as 3 point, and if sum is 10 points, conclusion is made about non-destructive cholecystitis, and if sum is from 10 points conclusion about destructive cholecystitis is made.
EFFECT: application of invention allows to increase accuracy of diagnostics of bile bladder tissue destruction in acute cholecystitis.
SUBSTANCE: invention relates to pharmacology. Medication for brain cell protection contains dominant protein extract from placenta tissue, obtained by chromatographic separation of protein fraction of 8-10 components with total molecular weight from 15 to 200 kDa, 70-80% of fraction consisting of protein with molecular weight about 70 kDa. In order to obtain said medication powdered placenta is extracted by means of alkalescent buffer in presence of inhibitors of proteolytic protein cleavage and centrifuged. Centrifugate is passed through chromatographic column with anion-exchange carriers balanced by means of the same buffer, column is washed from unbound material, proteins are eluted with neutral salt solution. Collected material is diluted with water bringing pH to subacid value and diluted substrate is successively passed through column with anion-exchange carrier and column with cation-exchange carrier, balanced with buffer solutions to pH about 6.0. Final product is obtained after sterilisation.
EFFECT: method application allows to obtain efficient medication of high purity, normalising nervous system functions.
3 cl, 1 tbl, 4 ex
SUBSTANCE: invention relates to field of medicine, namely to surgery. Blood is sampled from cubital vein in patients with acute pancreatitis on 1-3 and 7-10 day from disease beginning. Level of blood serum myoglobin is determined by method of performing reaction of passive hemagglutination. If level of blood serum myoglobin on 1-3 day increase from 95 to 128 ng/ml, acute fatty pancreatic necrosis is diagnosed, if myoglobin level in blood serum is higher than 128 ng/ml, hemorrhagic pancreatic necrosis is diagnosed, and if level of myoglobin in blood serum on 7-10 day increases higher than 256 ng/ml, hemorrhagic pancreatic necrosis in stage of infection of pancreatic necrosis nidus is diagnosed.
EFFECT: method allows to correct drug therapy and individually ground tactics of treating patients with acute pancreatitis depending on severity of disease form and presence of infection.
1 tbl, 3 ex
FIELD: medicine, ophthalmology.
SUBSTANCE: in lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula: At PC value being below 10.0 one should predict favorable disease flow, and at PC value being above 10.0 - unfavorable flow.
EFFECT: higher accuracy of prediction.
FIELD: forensic medicine.
SUBSTANCE: for the purpose to detect the sequence of applied lesions at availability of several wounds, scratches and ecchymoses on a cadaver one should study the activity of alkaline peptides isolated out of affected tissue by the impact of blood neutrophils of healthy donors upon phagocytosis. Moreover, the highest stimulating effect belongs to the peptides isolated out of the lesion applied earlier. The method enables to detect the sequence of applied lesions more accurately and differentiate the repeated lesion applied 5 min later, or more.
EFFECT: higher efficiency and accuracy of detection.
2 ex, 2 tbl
FIELD: medicine, biochemistry.
SUBSTANCE: in blood serum one should detect the level of lactoferrin and biliary acids. At their ratio being equal to 5-17 it is necessary to detect chronic hepatitis of high activity.
EFFECT: higher accuracy of detection.
SUBSTANCE: method involves determining cathepsin D activity in endometrium bioptate. The value being equal to or less than 0.1 units of enzymatic activity per hour, external genital endometriosis is diagnosed.
EFFECT: high accuracy of diagnosis.
SUBSTANCE: method involves studying lactoferrin content in blood serum and peritoneal exudates in postoperative period every day during the first three days. Lactoferrin concentration in blood serum being concurrently reduced by 0.02 mcmole/l or less and increasing lactoferrin concentration in peritoneal exudates by 0.04 mcmole/l or more, enteric detoxication is considered to be effective.
EFFECT: high quality of estimation.
SUBSTANCE: method involves determining plasminogen/plasmin, α2-macro-globulin, α1-antitripsin content at the first, third, fifth and tenth day. The plasminogen/plasmin level being equal to 66-74 mcmole/l or 100-120 mcmole/l, α2-macro-globulin level of 2.7-3.0 mcmole/l, α1-antitripsin content of 2.38-3.2 mcmole/l, systemic inflammatory response to purulent infection, light severity degree endotoxicosis is diagnosed and favorable disease outcome is predicted. The plasminogen/plasmin level being equal to 50-65 mcmole/l or 125-160 mcmole/l, α2-macro-globulin level of 2.3-2.6 mcmole/l, α1-antitripsin content of 3.3-4.0 mcmole/l, sepsis with organ and system dysfunction, moderate severity degree endotoxicosis is diagnosed and septic complication availability and lingering disease development course is predicted. The plasminogen/plasmin level being equal to 39-40 mcmole/l, α2-macro-globulin level of 1.58-2.08 mcmole/l, α1-antitripsin content of 5.0-6.2 mcmole/l, severe sepsis, septic shock, severe degree endotoxicosis is diagnosed and unfavorable disease outcome is predicted.
EFFECT: high accuracy of diagnosis.
FIELD: medicine, biochemistry.
SUBSTANCE: at testing one should precipitate high-molecular compounds with acetonitrile and register supernatant's spectral characteristics. Supernatant should be applied onto a paper filter, dried and put into solution containing aromatic aldehyde, acetone and concentrated hydrochloric acid taken at weight ratio of 70:5:1 to be kept for 2-3 min. Then it should be once again dried up to detect qualitative and semiquantitative content of oxidized tryptophan metabolites by intensity and chromatic shades. Moreover, by chromatic shades of yellow dyeing it is possible to detect the content of hydroxylated metabolites and by chromatic shades of violet dyeing - that of unhydroxylated ones.
EFFECT: higher significance of detection.
FIELD: medicine, anesthesiology, resuscitation.
SUBSTANCE: in patients one should study the content of lactoferrin in peritoneal exudates during the 1st d of postoperational period and at decreased value being below 3500 ng/ml on should predict unfavorable result. The suggested method provides correction of possible postoperational complications that deteriorate the flow of peritonitis and lead to lethal result.
EFFECT: higher accuracy of prediction.
FIELD: veterinary medicine.
SUBSTANCE: method involves determining low and middle molecular mass substances content in blood plasma and erythrocytes and general blood plasma albumin concentration. Integral index is calculated on basis of obtained values using formula II=100*S238-298(plasma)/S238-298(erythrocytes)*GAC, where S238-298(plasma) and S238-298(erythrocytes) are the low and middle molecular mass substances content in blood plasma and erythrocytes, respectively, determined from area of figures restricted by spectral curves in wavelength range of 238-298 nm and abscissa axis (conditional units2); GAC is the general blood plasma albumin concentration (g/l). The value being from 2.1 to 3.0, the first endotoxicosis degree is diagnosed. The value being from 3.1 to 4.5, the second endotoxicosis degree is diagnosed. The value being from 4.5 to 6.0, the third endotoxicosis degree is diagnosed. The value being greater than 6.0, the fourth endotoxicosis degree is diagnosed. The normal value is equal to 0.5-2.0.
EFFECT: high accuracy of diagnosis.
1 dwg, 1 tbl
SUBSTANCE: method involves separating blood serum proteins into fractions, determining albumins and alpha-2-globulins content and controlling their content changes during the disease development process. Gamma-globulin content is determined in per cent ratio with respect to total protein quantity. Then, changes in the fractions content are controlled from the first to the third week. Albumin content being in norm and alpha-2-globulins content becoming greater to the end of the first week by 30-50% when compared to normal value and dropping to norm at the second week end and gamma-globulin content increasing from norm by 10-30% to the second or the third week, high inflammatory process activity is to be diagnosed. Albumin content dropping by 10-30% from normal value at the second week, alpha-2-globulins content growing by 10-20% of norm and gamma-globulin content dropping by 30-50% at the second or the third week when compared to norm, low inflammatory process activity is to be diagnosed.
EFFECT: high accuracy and reliability of diagnosis.