Method of express estimation of hemostasis system functional state

FIELD: medicine.

SUBSTANCE: blood sample is placed in capillary, in whose walls installed are electrodes connected to frequency generator and registering unit, blood electric conductivity is measured at the moment of passing through it of alternating current with frequency 200 Hz, electric coagulogram is registered and used to determine chronometric and amplitude characteristics: A - amplitude of functional curve decline, mV; N - time of functional curve decline to minimal value in minutes. If value of A/T index decreases or increases with respect to normal, conclusion about hemostatic disorders is made. If value of A/T index equals 3-5 - hemostasis state is evaluated as normal, if A/T value is lower than 3, hypocoagulation is determined, and if A/T value is higher than 5 - hypercoagulation.

EFFECT: application of the method makes it possible to obtain data about hemostasis system state in real time mode, without injuring form blood elements in investigated microvolumes of blood, thus making it possible to increase accuracy, self-descriptiveness and efficiency of hemostasis state evaluation and to carry out correction of performed therapy without delay.

4 dwg, 3 ex

 

The invention relates to medicine, specifically to instrumental methods of assessing the functional state of the hemostatic system.

The highest value of the functional state of the hemostatic system has to maintain a normal blood flow, prevention and edema hemorrhage, thrombosis, ischemia and myocardial bodies, protection against the dissemination of bacteria and toxins from the lesions. This is the most important General biological value of the hemostatic system and the role of its disorders in the pathogenesis of the disease [3].

All of the above determines the high significance of hemostatic laboratory tests in medical practice. The particular importance of monitoring blood coagulation system associated with the active application in clinical practice of modern high-performance anti direct and indirect actions, antiplatelet agents, and thrombolytics. In these cases, therapeutic effect is achieved by correction of hemostasis disorders [1, 3].

Early and accurate diagnosis of disorders of the hemostatic system is a factor that depends on the timely treatment of the patient and the beginning of the preventive measures for the prevention of complications [3, 6].

The difficulty of studying the state of hemostasis due to the fact that this system is it has high biological variability and instability of its factors the complexity of extracting the determined parameter of the cascade of interrelated reactions, inability, in many cases, direct measurements of concentrations, as well as the ability methodological and instrumental unification [3].

Today there is a wide range of methods to assess the functional state of the blood coagulation system. However, to get information had a high diagnostic importance of the correct selection of a set of tests, often requiring them to produce special qualifying examination. A detailed examination of all parts of hemostasis cumbersome and time-consuming, requires large quantities of blood, not moving fast enough and is mainly research, not applied[2, 3, 4].

The desire for more efficient, targeted and operational study of the hemostatic system is quite understandable and justified. In this regard, the benefits can be methods of the graphic registration of the processes of blood coagulation and fibrinolysis.

The known method for the instrumental assessment of the functional state of the hemostatic system [5], which consists in measuring the conductivity of the blood by passing through it current. The blood sample is placed in a temperature-controlled cell, immersed in her plate electrodes, United chastoty generator and the registration unit, and measure the electrical conductivity of the blood by passing through it an alternating current with a frequency of 200 Hz. The recording electrocochleography varies from 15 to 100 minutes, and the image of the functional curve electrocochleography determine the timing and amplitude parameters characterizing the state of the hemostatic system.

This method is the closest to the claimed and selected as a prototype.

The disadvantage of the prototype method is that the study of the functional state of the hemostatic system in this way use large amounts of blood, and the registration process up to 100 minutes is not possible to monitor the process gemokoagulyatsii in real time.

The objective of the proposed method is able to track the process of gemokoagulyatsii in real time in the studied micro-volume of blood, without the trauma of formed elements of the blood by reducing the mechanical impact on them.

The problem is solved in that place the blood sample in the capillary tube, the walls of which is placed the electrodes connected to the frequency generator and the registering unit, measure the conductivity of the blood by passing through it an alternating current with a frequency of 200 Hz. Then write electrocoagulator is determined by her timing and amplitude characteristics: - the amplitude reduction of the functional curve, mV; T - time reduction of the amplitude functional curve to the minimum value in minutes. On the obtained image of the functional curve appreciate the level of activation of the blood clotting process (A/T), which is defined as the maximum slope of the functional curve. With a decrease or increase the value of the indicator A/T relative norms assess abnormalities in the hemostatic system. When values of A/T, equal 3-5, assess the condition of hemostasis as the norm, when values of A/T less than 3 determine the hypocoagulation, and when values of A/T over 5 - hypercoagulation.

New in the proposed method is the use of micro-volume blood was placed in a special capillary, the definition of the index of the intensity of activation of blood coagulation And/T and assessment of hemostasis as the norm when A/T is equal to 3-5 as hypocoagulation, when values of A/T less than 3, as hypercoagulation, when values of A/T more than 5.

Using the proposed method for the rapid assessment of the functional state of the hemostatic system can quickly in real time (in minutes) from a drop of blood taken from a finger, to monitor the process gemokoagulyatsii the patient and immediately make a correction therapy. This is a convenient and safe way under what the actual content of the hemocoagulation indicators in therapeutic range, that, in turn, reduces the likelihood of complications (thrombosis, embolism or hemorrhage).

The essential features of the method showed in the inventive combination of new properties that are not explicitly derived from the prior art in this field and obvious to the expert.

Identical set of features not found in the patent and medical literature.

The proposed method can be used in medical practice to improve the quality of treatment.

The invention will be clear from the following description and the attached drawings.

Figure 1 shows the device, which is carried out rapid assessment of the functional state of the hemostatic system.

Figure 2 presents electrocoagulating patient A.

Figure 3 presents electrocoagulating patient,

Figure 4 presents electrocoagulating patient W,

where a is the amplitude reduction of the functional curve, mV; T - time reduction of the amplitude functional curve to the minimum value in minutes.

The proposed method for the rapid assessment of the functional state of hemostasis is performed using the device (figure 1), consisting of a capillary 1, the wall 2 of which is placed the electrodes 3 through wires 4 connected to the contact plate 5, the VM is tirovannoj on the opposite wall of the capillary, connected to the frequency generator 6 and the recording unit 7.

A sample of capillary blood volume (0.05 to 0.1 cm3) is placed in the lumen of the capillary 1. Next, using electrodes 3 are passed through the blood of alternating current with a frequency of 200 Hz and measure the electrical conductivity of blood. The results obtained in real time in digital form on the display of the recording unit 7, together with an account of the functional curve (electrocochleography). Then carry out the analysis with further interpretation of the obtained graphic images of the functional curve (electrocochleography), where a is the amplitude reduction of the functional curve, measured in mV; T - time reduction of the amplitude functional curve to the minimum value in minutes; A/T indicator of the intensity of activation of the blood clotting process (Hypo-, normo-, hypercoagulation), which is defined as the maximum slope of the functional curve. When values of A/T 3-5 estimated as the norm, while reducing the magnitude And/T less than 3 estimate as a hypocoagulation, and at higher values of A/T more than 5 assess as hypercoagulation.

Example 1. A patient of 24 years, the case history No. 627, was admitted to the Department therapy clinic of the Institute of pharmacology WITH the RAMS. The diagnosis of autonomic neuropathy on hypotonic type. Prolaps MK I century, regurgitation 1 tbsp. NK - 0. To assess functional the condition of hemostasis patient studies of the blood coagulation system using conventional techniques.

The following results are obtained.

Complete blood count: hemoglobin - 126 g/l, erythrocytes - 4,21 T/l CPU - 0,90, hematocrit 45%, leucocytes - 5,2 G/l, ESR - 7 mm/h, platelets - 350 G/L. Leukocyte formula: p/I neutrophils - 0%, segmented neutrophils - 50%, eosinophils - 5%, basophils - 0%, lymphocytes - 37%, monocytes - 8%.

The coagulation tests: APTT - 38,0, prothrombin time and 15.3, fibrinogen General - 2.8 g/l; fibrin monomer complex - 3.5 mg/100 ml, fibrinolytic activity - 240 minutes

The study of platelet aggregation

Inductors aggregationThe transmittance, %The size of the aggregates, UE
Spontaneous aggregation00,87
ADP, 1.25 mg/ml225,8
ADP, 2.5 mg/ml286,4
ADP, 5 mg/ml327,2
Adrenaline, 2.5 mg/ml477,8
Adrenaline, 5 mg/ml526,6
Collagen695,9

Conclusion: Indicators of platelet aggregation in normal limits.

Additionally, we performed a study using the proposed device, the following results are obtained: A - 10 mV, T - 3.2 min, A/T - 3,1.

The findings of the study variance in the functional state of blood coagulation was not detected.

Example 2. Patient, 32 years of age, medical history, No. 474, was admitted to the Department therapy clinic of the Institute of pharmacology WITH the RAMS. The diagnosis of ischemic heart disease: angina, FC-II. NK - 0-I. Patient before admission to the hospital for a long time took cardiomagnyl 75 mg/day. To assess the functional state of hemostasis patient studies of the blood coagulation system using conventional techniques.

The following results are obtained.

Complete blood count: hemoglobin - 139 g/l, erythrocytes - 4,37 T/l CPU - 0.95, and a hematocrit of 41%, leucocytes - 6.7 G/l, ESR - 12 mm/h, platelets - 332 G/l Leukocyte formula: p/I neutrophils - 0%, segmented neutrophils - 48%, eosinophils - 1%, basophils - 0%, lymphocytes - 44%, monocytes - 7%.

The coagulation tests: APTT - 40,0, prothrombin time - 24,0, fibrinogen common - 4.0 g/l; fibrin monomer complex - 4,5, fibrinolytic activity - 210 minutes

The study of platelet aggregation

Inductors aggregationThe transmittance, %The size of the aggregates, UE
Spontaneous aggregation00,5
ADP, 1.25 mg/ml155,0
ADP, 2.5 mg/ml205,5
ADP, 5 mg/ml255,3
Adrenaline, 2.5 mg/ml74,5
Adrenaline, 5 mg/ml104,5
Collagen656,0

Conclusion: Significant reduction in the degree adrenaline-platelet aggregation on the background of antiaggregatory treatment.

Additionally, we performed a study using the proposed device, the following results are obtained: A - 25 mV, T - 12 min, A/T 2,1.

The findings of the study of the functional state of blood coagulation revealed the state gipokoagulyatsii.

Example 3. Patient W. 64 years, case history No. 63, was admitted to the Department therapy clinic of the Institute of pharmacology WITH the RAMS. Diagnosed with Obliterating atherosclerosis of the lower extremities. Occlusion of the iliac-femoral segment on the right. To assess the functional state of hemostasis patient studies of the blood coagulation system using conventional techniques.

The following results are obtained.

Complete blood count: hemoglobin - 160 g/l, erythrocytes - the ceiling of 5.60 T/l, the CPU is 0.86, the hematocrit of 52%, leucocytes - 7,3 G/l, ESR - 2 mm/h, platelets - 233 G/l Leukocyte formula: p/I neutrophils - 0%, segmented neutrophils - 63%, eosinophils - 1%, basophils - 0%, lymphocytes 32%, monocytes - 4%.

The coagulation tests: APTT - 27,0, prothrombin time - 13,0 with, fibrinogen General - 5,1 g/l; fibrin monomer complex - 21,0, fibrinolytic activity - 240 minutes

The study of platelet aggregation

tr>
Inductors aggregationThe transmittance, %The size of the aggregates, UE
Spontaneous aggregation56,0
ADP, 1.25 mg/ml5010,3
ADP, 2.5 mg/ml7012,5
ADP, 5 mg/ml7013,3
Adrenaline, 2.5 mg/ml8514,8
Adrenaline, 5 mg/ml8514,5
Collagen9515,0

Conclusion: Increased aggregation activity of platelets.

Additionally, we performed a study using the proposed device, the following results are obtained: A - 37 mV, T - 6 min, A/T - 6,2.

The findings of the study of the functional state of blood coagulation revealed hypercoagulable state. The patient is recommended to take cardiomagnyl 75 mg/day.

In total, we used blood samples of 40 patients divided into 3 groups: 1 - normal (13 patients), 2-I - hypercoagulation (11 patients) and 3-I - hypocoagulation (16 patients).

The offered way of a rapid assessment of the functional state of the hemostatic system allows you to obtain the status of the hemostatic system in real time, without the trauma of blood cells in the studied micro-volume of blood that can increase the accuracy, informativeness and operatives the assessment of hemostasis.

The list of used literature

1. Scenarion, Apomu. Diagnostics and controlled therapy of disorders of hemostasis. - Moscow: "Novamed", 2001. - 296 S. (pp.178-192).

2. ISO. Manual of laboratory diagnostic methods. - M.: GEOTAR. Media, 2007. - 800 S. (s-340).

3. Apomu. Pathology of hemostasis. Principles and algorithms of clinical laboratory diagnostics. - SPb.: Format, 2006. - 208 S. (p.9-11; 15-120; 139-154).

4. Medical laboratory technology and diagnosis: Reference. Medical laboratory technology / Edited. editor Prof. Aeacides. - SPb.: InterMedia, 1999. - 656 S. Il. (s-284).

5. The description of the patent No. 2282855. The method of assessing the functional state of the hemostatic system. III, Wearechange, Waisanen, Mnepismo, Waipahu, Mberengwa. Published: 27.08.2006. Bull. No. 24.

6. WRX. Thrombosis in cardiology practice. 2nd ed., extra - SPb.: Nevsky Dialect, M.: Publishing house of the BINOMIAL, 2001. - 125 p: Il. (p.51-60).

The way of a rapid assessment of the functional state of the hemostatic system, including the measurement of electrical conductivity of the blood by passing through it an alternating current with a frequency of 200 Hz, the recording electrocochleography and determination on her timing and amplitude characteristics And the amplitude reduction of the functional curve, mV; T - time reduction of the amplitude functional curve to the mini is real value in minutes, characterized in that the blood sample is placed in a capillary tube, the walls of which is placed the electrodes connected to the frequency generator and the registering unit, measure the conductivity of the blood and the image of the functional curve appreciate the level of activation of the blood clotting process A/T, a/T, equal 3-5, assess the condition of hemostasis as the norm, when values of A/T less than 3 determine the hypocoagulation, and when values of A/T over 5-hypercoagulation.



 

Same patents:

FIELD: medicine.

SUBSTANCE: in patients with IHD before therapy with acetylsalisylic acid (ASA) ADP-induced and ASA-dependent platelet aggregation are examined and by their difference value of coefficient of aggregation inhibition (CAI) is calculated. CAI value <24% testifies to resistance to ASA, if CAI ≥24% - about sensitivity to ASA.

EFFECT: method ensures high prediction accuracy and makes it possible to prevent development of undesirable coronary events in IHD patients.

1 tbl, 4 ex, 4 dwg

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to haematology, and orthopaedics. The intravascular thrombocyte activity correction in children with scoliosis requires the prescription for at least 6 months of a therapeutic complex which involves daily therapeutic physical training, two courses of massage and daily swimming for at least 20 minutes a day.

EFFECT: method allows to normalise intravascular thrombocyte activity in children aged 8-12 with scoliosis, to improve tissue microcirculation considerably, to optimise growth and development of skeleton and internals.

7 ex

FIELD: medicine.

SUBSTANCE: invention belongs to medicine, notably to haematology and orthopaedics. For thromboplastin synthesis correction in children 8-12 years old with scoliosis is prescribed complex of treatment, including daily remedial gymnastics, two courses of massage and daily swimming for at least 20 minutes a day, during 6 months.

EFFECT: method enables thromboplastin synthesis normalisation in children with scoliosis, significantly sanitate children with scoliosis due to improvement of tissues' microcirculation.

7 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to aggressive medical therapy, resuscitation science, critical care medicine, laboratory diagnostics and can be used by resuscitators, intensivists, laboratory doctors for well-timed diagnosis and consequently, for individualised aggressive medical therapy of acute disseminated intravascular coagulation. The integrated assessment of links of haemostatic system and the clinical appraisal of organ dysfunction are applied in a measurer, and when observing structural hypercoagulation characterised by fibrinogen level increase, thrombocyte activity increase, growth of soluble fibrin complex (SFC) level, and also when observing chronometric hypercoagulation characterised by time tests, palette-derived factor 4 activity (P4) with manifested petechial haemorrhage and organ dysfunction, and coagulation cascade activation with underlying depression of antithrombin III and protein C, a hypercoagulation stage of acute DIC is diagnosed. Chronometric hypercoagulation by Activated partial thromboplastin time (APTT), INR, fibrinogen and P4 with manifested signs of structural hypocoagulation by thrombin time prolongation and D-dimer activity increase with underlying further intensification of anticoagulant system deficiency, progression of target organs dysfunction and mixed haemorrhage show a transitive stage of acute DIC. If observing said hypocoagulation changes and disturbed fibrinolytic activity with prevailing either decompensated organ and tissue dysfunction, or hemorrhagic syndrome up to system haemorrhages, or their combination with hemorrhagic syndrome characterised by polymorphism of clinical picture and localisation: petechial-haematoma haemorrhage at the stress-induced stomach ulcers, hematuria, a coagulopathy stage of acute DIC is diagnosed that is characterised either by depression of fibrinolysis, or preserved fibrinolytic activity, or by activation of secondary fibrinolysis, or by acute primary fibrinolysis.

EFFECT: method allows optimising classification of acute disseminated intravascular coagulation, improving diagnostic significance of the classification and simplifying a diagnostic prospecting which provide a basis to consider the staging of acute disseminated intravascular coagulation.

1 dwg, 4 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to haematology and orthopaedics. Correction of blood microvesicle level in children aged 8-12 with scoliosis is ensured by the prescription of a therapeutic complex that involves daily therapeutic physical training, two courses of massage and daily swimming for at least 20 minutes a day, for at least 6 months.

EFFECT: method enables correcting blood microvesicle level in children aged 8-12 with scoliosis, optimising microcirculation in tissues, normalising functions of the internal organs, growth and development of a child.

7 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to haematology, and orthopaedics. Thrombocyte aggregation normalisation in children aged 8-12 with scoliosis is ensured by the prescription of a therapeutic complex that involves daily therapeutic physical training, two courses of massage and daily swimming for at least 20 minutes a day. The therapeutic complex is performed for at least 6 months.

EFFECT: method improves the effectiveness of thrombocyte aggregation correction in children aged 8-12 with scoliosis.

9 ex

FIELD: medicine.

SUBSTANCE: blood is drawn off, stabilised to recover thrombocyte-enriched plasma (TEP) and applied on a slide where thrombocyte aggregation test with a number of inducers is performed. In this environment, the visual evaluation of thrombocyte aggregation allows creating a real model of blood flow with small volume (0.02 ml.) of plasma and small volume of inducers with using minimum mutually potentiating concentrations of inducers, with necessary application of collagen in the combinations and calculation of "СЗИАК" the value of which is used to observe fine disorders of thrombocyte aggregation.

EFFECT: improved clinical effectiveness.

4 dwg, 4 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: invention can be used to evaluate thrombocyte aggregation in the intravascular-related conditions to diagnose thrombocyte hypo- and hyperaggregation in physiological and pathological statuses. A blood sample is drawn, stabilised with 3.8% sodium citrate, divided into plasma and erythrocytes by centrifugation. That is followed with the thrombocyte aggregation test with simultaneously addition of at least three aggregation inducers chosen of the group including ADP, adrenaline, collagen, thrombin. The time of aggregate generation while adding the aggregation inducers is recorded with calculating the average aggregation within a damage area (AADA). If the AADA is 20.0-23.75 sec, the thrombocyte aggregation is considered to be normal. The AADA 12.1-19.9 sec indicates the risk of hyperaggregation; while the value 12.0 sec and less indicates high thrombocyte aggregation with potential thromboses in the nearest future. If the AADA is 23.76-29.9, there is a risk of hypoaggregation; the values 30.0 sec and higher show hypoaggregation with developing hemorrhagic diathesis in the nearest future.

EFFECT: application of the method allows diagnosing thrombocyte hypo- and hyperaggregation in small plasma amount.

3 dwg, 3 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to gynaecology, and can be used for prediction of the clinical course of early postoperative destructive processes following conservative surgeries of tubo-ovarian growths. It involves the blood analysis in the patients with inflammatory diseases in uterine appendages after conservative laparoscopic surgeries. Every day during three postoperative days, blood is analysed for soluble fibrin complexes (SFC) and fibrinogen (FG) to calculate a destruction coefficient D by formula: D=SFC/FG. Where: SFC is an amount of soluble fibrin complexes in blood, mg/%; FG is an amount of fibrinogen in blood, g/l; and its values exceeding 2.9 enable to predict an unfavourable clinical course of the postoperative period to prescribe expansion of the operation extent; the value D equal to 2.9 preserved at the same level or decreased shows a favourable clinical course of the postoperative period.

EFFECT: method allows detecting developing destructive process that improves accuracy of predicting the clinical course of the disease in the early postoperative period and allows for well-timed correction of the administered therapy and enlarging the operation extent if required.

2 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, particularly to gynaecology and can be used for express diagnostics of necrotic suppurative tissue destruction in inflammatory diseases of uterine appendages. The clinical signs are determined and in observed inflammatory diseases of uterine appendages, blood is sampled to analyse blood plasma for the content of fibrin-monomer complexes (FMC) and fibrinogen count (FG), and to calculate the destruction factor D by formula: D=FMC/FG, where: FMC is soluble fibrin-monomer complex count in blood, mg/%, FG is fibrinogen count in blood, g/l. If D is equal to and more than 2.9, necrotic suppurative destruction in uterine appendages is considered to be observed, and the values less than 2.9 show the absence of destruction in uterine appendages.

EFFECT: method allows reducing time and improving quality of diagnostics that provides a basis for well-timed therapeutic approach adequate to the clinical situation.

2 ex

FIELD: medicine, laboratory diagnostics.

SUBSTANCE: the suggested studying should be carried out on the glass simultaneously with several inductors by applying minimal inter-taking antilogarithms concentrations of aggregation inductors which correspond at double combination of inductors: ADP 5.0 x 10-8 M, adrenaline 3.0 x 10-9, collagen - dissolving the main suspension 1:8, thrombin 0.075 U/ml; at triple combination of inductors: ADP 10-9 M, adrenaline 10-9, collagen - dissolving the main suspension 1:9, thrombin 0.060 U/ml. The development of aggregation means thrombocytic activation in patients with arterial hypertension at metabolic syndrome. The method enables to evaluate the changes of thrombocytic functional state with combination of inductors more probably present in area of vascular lesion by applying minimal necessary concentrations that develops real conditions at hemostatic initiation in human vessels.

EFFECT: higher efficiency of studying.

3 dwg, 3 ex, 2 tbl

FIELD: medicine.

SUBSTANCE: method involves checking consciousness, blood coagulation state, peripheral blood leukocytes number, K+ ions, bilirubin, fibrinogen, hemolysis and hemoglobinuria availability, prothrombin index and exotoxic shock development. Each value is calculated in points as follows. Lucidity is evaluated as -2 points; depression - +3 points; coma - +6 points; lack of changes in blood coagulation system - -2 points; coagulation availability without clinical injuries - +2 points; coagulopathy with clinical manifestation signs - +19 points; K+ ions concentration being less than 3.0 mmole/l - +3 points, from 3.1 to 3.5 mmole/l - -5 points, from 3.6 to 5.0 mmole/l - 0 points, greater than 5.0 points - +7 points, failure in determining K+ ions concentration - 0 points; hemolysis availability - +6 points, its lack - -3 points; hemoglobinuria availability - +8 points, its lack - -1 points; leukocytes number being less than 12.0x109/l - -2 points, from 12,1 to 18.0x109/l - 0 points, higher than 18.0x109/l - +8 points; hourly urine output being less than 30 ml/h - +6 points, greater than 30 ml/h - -2 points; bilirubin content being less than 31 mcmole/l - -2 points, from 30.1 to 50.0 mcmole/l - 0 points, greater than 50.0 mcmole/l - +2 points, failure in determining bilirubin content due to hemolysis being available -+6 points; prothrombin index being equal to or less than 60% - +3 points, greater than 60% - 0 points, failure in determining prothrombin index due to hemolysis being available - +12 points; fibrinogen concentration in blood plasma being less than 2.1 g/l - +4 points, from 2.1 to 4.0 g/l - -1 point, from 4.1 to 6.0 g/l - +1 point, failure in determining fibrinogen concentration due to erythrocyte hemolysis being available - +13 points; exotoxic shock development - +9 points, its lack - -1 point. The points are summed up. The value being greater than +13, admission for treatment in resuscitation department is indicated. The value being less than -13, admission for treatment in therapeutics department is indicated. The value being from -13 to +13, resuscitation expert consultation is advised.

EFFECT: high evaluation accuracy.

3 tbl

FIELD: medicine, laboratory diagnostics.

SUBSTANCE: one should evaluate the time for clotting of plasma under testing in phospholipid-dependent test, moreover, one should apply high- and low-sensitive thromboplastin reagents to lupus anticoagulant to calculate the ratio of indices of prothrombin time prolongation and at its value being either equal to or above 1.1 one should diagnose APS.

EFFECT: shortened terms of research.

1 ex, 4 tbl

FIELD: medicine.

SUBSTANCE: method involves analyzing symptoms manifesting initial disseminated intravascular blood coagulation syndrome danger like burn area, availability of upper air passages burn, shock with its severity degree taken into consideration, sepsis development; clinical manifestations of disseminated intravascular blood coagulation syndrome like lung, kidney, liver function insufficiency, cerebral dysfunction, local and multiple hemorrhages, thrombosis, infarction; homeostasis system laboratory analysis data, hyper- and hypocoagulation based on chronometry test data, number of blood platelets, fibrin-monomer complexes, D-dimers, activity of antithrombin III, C and S proteins, XIIa-dependent fibrinolysis plasminogen content, availability of injured erythrocytes, combinations of laboratory tests for recognizing disseminated intravascular blood coagulation syndrome. Each sign under consideration receives a number of points corresponding to its diagnostic significance and integral value is calculated DIBCSIV=(X1+X2+…+Xn)/n, where n is the number of signs taken into consideration. DIBCSIV value equal to 1.0-1.5 units shows physiological norm. The value being between 1.6 and 2.5 units, light disseminated intravascular blood coagulation syndrome is diagnosed. The value being between 2.6 and 3.5 units, disseminated intravascular blood coagulation syndrome of medium severity is diagnosed; 3.6-4.5 points to one heavy severity degree; 4.6 and greater indicates highly severe case of disseminated intravascular blood coagulation syndrome.

EFFECT: high accuracy and objectiveness in differentiating syndrome severity degrees.

1 tbl

FIELD: medicine, diagnostics.

SUBSTANCE: one should study blood components to detect anticoagulant-fibrinolytic activity. Moreover, patient's blood should be sampled: in whole blood one should detect the presence of affected erythrocytes and evaluate the quantity of thrombocytes, in plasma it is necessary to study the activity of antithrombin III, XIIa-dependent fibrinolysis, the content of soluble fibrin-monomeric complexes, in blood serum of the sample taken one should detect the concentration of urea, creatinine, sodium, albumin, total cholesterol and the activity of aspartate aminotransferase, moreover, one should calculate integral value of renal-hepatic deficiency, to put corresponding point for the degree of parameters under testing, then one should calculate integral value of disseminated intravascular clotting (IVDIC) and at its value being 6.3 U and more DIC-syndrome should be diagnosed, moreover, at IVDIC value ranged 6.3-10.1 U it is possible to diagnose latent DIC-syndrome, at 10.2-14.6 - subacute DIC-syndrome and at 14.7 and higher - acute DIC-syndrome should be concluded.

EFFECT: higher accuracy and efficiency of diagnostics.

4 ex, 2 tbl

FIELD: medicine, obstetrics.

SUBSTANCE: the present innovation deals with predicting disadaptive processes in women in dynamics of menstrual cycle. During menstrual cycle beginning since the 1st d to the 21st d one should detect the dynamics for alteration in coefficient of activity of syntoxic adaptation programs (CASAP), calculated by the following formula:

where CST - concentration of blood serotonin, AAT-III - activity of antithrombin III, Aaoa - total antioxidizing activity of plasma, CCD8+ - concentration of T-suppressors, Cad - concentration of blood adrenalin, Cα2MG - concentration of α2-macroglobulin, CMDA - concentration of malonic dialdehyde, CCD4+ - concentration of T-helpers. Moreover, normally CASAP value alters two-fold against the first day of the cycle - since 0.70 up to 1.40 on the 21st d of the cycle, at no alterations in CASAP value one should diagnose female disadaptive alterations leading to failed pregnancy. The innovation enables to perform diagnostics of disadaptive processes in women in dynamics of menstrual cycle followed by prognostic conclusion upon future pregnancy.

EFFECT: higher accuracy of diagnostics.

2 ex

FIELD: medicine.

SUBSTANCE: method involves determining spontaneous blood platelets aggregation and one induced by adrenalin and collagen, thrombocytospecific peptides activity of β-thromboglobulin and thrombocytic factor 4 in blood plasma.

EFFECT: high accuracy of diagnosis.

2 tbl

FIELD: medicine.

SUBSTANCE: method involves determining coagulating blood viscosity values like reaction period r, thrombin constant K, maximum amplitude MA, time T for forming fibrin-thrombocytic blood clot, spontaneous blood platelets aggregation intensity Ar, retraction and spontaneous clot lysis total FA. The r being within 5-7 min, Ar from -2 to -6 relative units, K being within 4-6 min, MA within 500-700 relative units, T within 40-60 min and FA equal to 10-20%, low inflammatory process activity is considered to be the case. The r being less than 5 min, Ar equal to -8 to -12 relative units, T less than 40 min and FA less than 10% with no changes in K and MA being observed, inflammatory process activity in chronic glomerulonephritis case is considered to be of high severity degree.

EFFECT: high accuracy of diagnosis; enhanced effectiveness of treatment method selection.

1 dwg

FIELD: medicine, clinical neurology, neurosurgery.

SUBSTANCE: one should study both activation and aggregation of thrombocytes in blood of carotid artery, at the quantity of thrombocytic active forms being above 70% and the number of aggregated thrombocytes being above 9.0% one should predict the development of cerebral ischemic lesion along with stable focal neurological symptomatology, and at the quantity of thrombocytic active forms being below 30% and the number of aggregated thrombocytes being below 8.0% it is possible to predict positive dynamics in the course of the disease mentioned without developing cerebral ischemic lesion.

EFFECT: higher accuracy of prediction.

2 ex

FIELD: medicine, clinical neurology, neurosurgery.

SUBSTANCE: one should study the level of von Willebrand's factor in patient's carotid artery blood. At its content being below 105% one should predict the development of repeated AICH. The innovation improved information value of testing due to possibility to obtain reliable prediction in latent period, as well.

EFFECT: higher accuracy of prediction.

2 ex, 1 tbl

Up!