Fluorescent method for determining calcium concentration on basis of discharged photoproteins

FIELD: measurement equipment.

SUBSTANCE: invention refers to biophysics. In order to determine calcium concentration on the basis of discharged photoproteins, bioluminescent reaction of photoproteins and calcium ions is performed, intensity of the solution fluorescence is measured, calibration dependence of fluorescence intensity on calcium concentration in double logarithmic coordinates is built and logarithm value of calcium concentration is determined as per the logarithm of the measured fluorescence intensity. Fluorescence is initiated with the light source after bioluminescent reaction is completed. Fluorescence intensity is measured at the specified length of excitation and recording wave.

EFFECT: method allows determining calcium concentration in various media as per fluorescence intensity of discharged photoproteins, which allows performing continuous measurements of calcium concentration in "in vivo" system.

2 dwg, 6 ex

 

The invention relates to the field of Biophysics and can be used in biological and medical research for the quantitative determination of calcium concentration in different environments.

There is a method of determining the concentration of calcium, based on the fact that oxalates (salts of oxalic acid (H2C2O4) itself oxalic acid forming salts of calcium white insoluble precipitates [APPCRASH, Ala. The course of analytical chemistry, quantitative analysis. Ed.: Moscow "Chemistry", 1982].

Known bioluminescent method for determining the concentrations of calcium. This method uses enzymes coelenterates - fetoprotein. The method is based on the fact that in the presence of calcium ions starts bioluminescent reaction, and the intensity of bioluminescence depends on the calcium concentration. [..Illarionov, L.A.Frank, V.A.Illarionova, V.S.Bondar, E.S.Vysotski, J.R.Blinks Recombinant obelin: cloning and expression of cDNA, purification, and characterization as a calcium indicator / J. Methods in enzymology. - 2000. No.. 277. - P.223-249]. In this method, 1 ml of a solution containing an unknown concentration of calcium, add 10 μl solution of 5·10-6M obelin the luminescent protein in 20 mm TrisHCl buffer, pH 7. Next, register the intensity of bioluminescence using biochemiluminescence. On the gauge dependence of the intensity of bioluminescence from the calcium concentration to determine the concentration of calliav test solution.

The disadvantages of this method is the inability to quantify calcium in systems in vivo and the need for chemical reaction for each dimension.

The technical result of the invention is to develop a method for determining the concentration of calcium in various media by fluorescence intensity discharged photoprotein.

This technical result is achieved by the fact that under the fluorescent method for determination of calcium concentration on the basis of the discharged photoproteins, including bioluminescent reaction fetoprotein and calcium ions, the measurement of the fluorescence intensity of the solution, the construction of gauge dependence of fluorescence intensity on the concentration of calcium in the double logarithmic coordinates and determine the value of the logarithm of the calcium concentration on the logarithm of the light intensity, it is new that the initiate fluorescence light source after completion of the bioluminescent reaction, measure the intensity of fluorescence at a given wavelength excitation and reception.

The inventive method uses fluorescence discharged photoprotein that allows continuous measurement of the concentration of calcium in the systems in vivo. This difference allows to make a conclusion on the compliance of the claimed those who practical solutions to the criterion of "novelty".

The features distinguishing the claimed technical solution to the prototype, not identified in other technical solutions in the study of this and related areas of technology and, therefore, provide the claimed solution according to the criterion of "inventive step".

The invention is illustrated by drawings:

Figure 1 presents the dependence of the fluorescence intensity in the spectra of emission discharged photoprotein obelin the luminescent protein from the calcium concentration. The wavelength of 310 nm excitation, wavelength registration 510 nm.

Figure 2 presents the dependence of the intensity of fluorescence excitation spectra discharged photoprotein obelin the luminescent protein from the calcium concentration. The wavelength registration of 470 nm, the wavelength of the excitation 350 nm.

Similar dependencies were obtained during the variation of viscosity when added to the test solution of glycerin with the formation of 1, 3, 5, 10% solution of glycerin in water.

The inventive method for the determination of calcium concentration on fluorescence intensity in the spectrum of the emission is as follows.

Example 1. Preparation of calibration graph for the determination of calcium concentration using photoprotein obelin the luminescent protein. In the in vitro system, at room temperature, 230 μl of an aqueous solution of calcium at different concentrations (5·10-9M, 10 -8M, 5 x 10-8M, 10-7M, 5 x 10-7M, 10-6M, 5 x 10-6M, 10-5M, 10-4M, 10-3M) add 20 μl of a solution containing 5 x 10-5M photoprotein obelin the luminescent protein, 20 mm TrisHCl buffer, or PIPES, pH 7. In each case, the solution is stirred thoroughly for 1 min to fully conduct bioluminescent reaction, which forms a fluorescent product called discharged photoprotein obelin. Fluorescence analysis was carried out under continuous irradiation of a solution of light. The fluorescence intensity in the spectrum of emission discharged obelin the luminescent protein is measured at a wavelength of registration in the range of 460-540 nm photoexcitation wavelength in the range 335-365 nm. Construct a calibration graph of fluorescence intensity in the spectrum of emission discharged obelin the luminescent protein from the calcium concentration in the double logarithmic coordinates (Fig 1).

Example 1.1. In the in vitro system, at room temperature, 230 ál sample, in which the need to determine the concentration of calcium, made of 20 μl of a solution containing 5 x 10-5M photoprotein obelin the luminescent protein, 20 mm TrisHCl buffer, or PIPES, pH 7. Mix thoroughly for 1 min Under continuous irradiation of light to measure the intensity of the fluorescence emission spectrum at wavelengths registration and arousal, which were used to build calibers is knogo graphics. Consider the logarithm of the fluorescence intensity. The resulting value of the logarithm of the intensity substituted in the calibration graph (the dependence of the fluorescence intensity in the spectrum of emission discharged obelin the luminescent protein from the calcium concentration in the double logarithmic coordinates) and find the corresponding value of the logarithm of the calcium concentration. To determine the molar concentration of calcium is necessary to build ten to the power value of the logarithm of the calcium concentration, was found in a calibration curve for obelin the luminescent protein.

Example 1.2. In the system of in vivo administered 0.1 mg coelenterazine per 1 g of cells. Coelenterazine, being hydrophobic, permeates through the membrane, contacting synthesized in the cell by apocalymon education photoprotein obelin the luminescent protein. Under continuous irradiation of light to measure the intensity of the fluorescence emission spectrum at wavelengths registration and arousal, which were used to build the calibration graph. Consider the logarithm of the fluorescence intensity. The resulting value of the logarithm of the intensity substituted in the calibration graph (the dependence of the fluorescence intensity in the spectrum of emission discharged obelin the luminescent protein from the calcium concentration in the double logarithmic coordinates) and find the corresponding value of the logarithm of the calcium concentration. That is predelete the molar concentration of calcium, you need to build ten to the power value of the logarithm of the calcium concentration, was found in a calibration curve for obelin the luminescent protein.

Example 2. Preparation of calibration graph for the determination of calcium concentration using fetoprotein aquarina. In the in vitro system, at room temperature, 230 μl of an aqueous solution of calcium at different concentrations (5·10-9M, 10-8M, 5 x 10-8M, 10-7M, 5 x 10-7M, 10-6M, 5 x 10-6M, 10-5M, 10-4M, 10-3M) add 20 μl of a solution containing 5 x 10-5M fetoprotein aquarina, 20 mm TrisHCl buffer, or PIPES, pH 7. In each case, the solution is stirred thoroughly for 1 min to fully conduct bioluminescent reaction, which forms a fluorescent product called discharged photoprotein acorin. Fluorescence analysis was carried out under continuous irradiation of a solution of light. The fluorescence intensity in the spectrum of emission discharged aquarina measured at a wavelength of registration in the range 440-530 nm photoexcitation wavelength in the range of 320-350 nm. Construct a calibration graph of fluorescence intensity in the spectrum of emission discharged aquarina on the concentration of calcium in the double logarithmic coordinates.

Example 2.1. In the in vitro system, at room temperature, 230 μl of sample, which you want to determine the concentration of calcium, made of 20 μl of a solution containing 5 x 10-5M fetoprotein aquarina, 20 mm TrisHCl buffer, or PIPES, pH 7. Mix thoroughly for 1 min Under continuous irradiation of light to measure the intensity of the fluorescence emission spectrum at wavelengths registration and arousal, which were used to build the calibration graph. Consider the logarithm of the fluorescence intensity. The resulting value of the logarithm of the intensity substituted in the calibration graph (the dependence of the fluorescence intensity in the spectrum of emission discharged aquarina on the concentration of calcium in the double logarithmic coordinates) and find the corresponding value of the logarithm of the calcium concentration. To determine the molar concentration of calcium is necessary to build ten to the power value of the logarithm of the calcium concentration, was found in a calibration curve for aquaria.

Example 2.2. In the system of in vivo administered 0.1 mg coelenterazine per 1 g of cells. Coelenterazine, being hydrophobic, permeates through the membrane, contacting synthesized in the cell by apoaequorin education fetoprotein aquarina. Under continuous irradiation of light to measure the intensity of the fluorescence emission spectrum at wavelengths registration and arousal, which were used to build the calibration graph. Consider logari the m fluorescence intensity. The resulting value of the logarithm of the intensity substituted in the calibration graph (the dependence of the fluorescence intensity in the spectrum of emission discharged aquarina on the concentration of calcium in the double logarithmic coordinates) and find the corresponding value of the logarithm of the calcium concentration. To determine the molar concentration of calcium is necessary to build ten to the power value of the logarithm of the calcium concentration, was found in a calibration curve for aquaria.

Example 3. Preparation of calibration graph for the determination of calcium concentration using fetoprotein litina. In the in vitro system, at room temperature, 230 μl of an aqueous solution of calcium at different concentrations (5·10-9M, 10-8M, 5 x 10-8M, 10-7M, 5 x 10-7M, 10-6M, 5 x 10-6M, 10-5M, 10-4M, 10-3M) add 20 μl of a solution containing 5 x 10-5M fetoprotein litina, 20 mm TrisHCl buffer, or PIPES, pH 7. In each case, the solution is stirred thoroughly for 1 min to fully conduct bioluminescent reaction, which forms a fluorescent product called discharged photoprotein Klitin. Fluorescence analysis was carried out under continuous irradiation of a solution of light. The fluorescence intensity in the spectrum of emission discharged litina esmeraude wavelength reception in the range of 400-500 nm and photoexcitation wavelength in the range of 320-350 nm. Construct a calibration graph of fluorescence intensity in the spectrum of emission discharged litina on the concentration of calcium in the double logarithmic coordinates.

Example 3.1. In the in vitro system, at room temperature, 230 ál sample, in which the need to determine the concentration of calcium, made of 20 μl of a solution containing 5 x 10-5M fetoprotein litina, 20 mm TrisHCl buffer, or PIPES, pH 7. Mix thoroughly for 1 min Under continuous irradiation of light to measure the intensity of the fluorescence emission spectrum at wavelengths registration and arousal, which were used to build the calibration graph. Consider the logarithm of the fluorescence intensity. The resulting value of the logarithm of the intensity substituted in the calibration graph (the dependence of the fluorescence intensity in the spectrum of emission discharged photoprotein litina on the concentration of calcium in the double logarithmic coordinates) and find the corresponding value of the logarithm of the calcium concentration. To determine the molar concentration of calcium is necessary to build ten to the power value of the logarithm of the calcium concentration, was found in a calibration curve for litina.

Example 3.2. In the system of in vivo administered 0.1 mg coelenterazine per 1 g of cells. Coelenterazine, being hydrophobic, penetrates through member is, contacting synthesized in the cell by abolition education fetoprotein litina. Under continuous irradiation of light to measure the intensity of the fluorescence emission spectrum at wavelengths registration and arousal, which were used to build the calibration graph. Consider the logarithm of the fluorescence intensity. The resulting value of the logarithm of the intensity substituted in the calibration graph (the dependence of the fluorescence intensity in the spectrum of emission discharged litina on the concentration of calcium in the double logarithmic coordinates) and find the corresponding value of the logarithm of the calcium concentration. To determine the molar concentration of calcium is necessary to build ten to the power value of the logarithm of the calcium concentration, was found in a calibration curve for litina.

The inventive method for the determination of calcium concentration on fluorescence intensity in the spectrum of the excitation is carried out as follows.

Example 4. Preparation of calibration graph for the determination of calcium concentration using photoprotein obelin the luminescent protein. In the in vitro system, at room temperature, 230 μl of an aqueous solution of calcium at different concentrations (5·10-9M, 10-8M, 5 x 10-8M, 10-7M, 5 x 10-7M, 10-6M, 5 x 10-6M, 10-5M, 10-4M, 10 M) add 20 μl of a solution containing 5 x 10-5M photoprotein obelin the luminescent protein, 20 mm TrisHCl buffer, or PIPES, pH 7. In each case, the solution is stirred thoroughly for 1 min to fully conduct bioluminescent reaction, which forms a fluorescent product called discharged photoprotein obelin. Fluorescence analysis was carried out under continuous irradiation of a solution of light. The intensity of the fluorescence excitation spectrum "Sa-discharged" obelin the luminescent protein is measured at a wavelength of excitation in the range 335-365 nm and registration on the wavelength in the range of 460-540 nm. Construct a calibration graph of fluorescence intensity in the spectrum of the excitation Sa-discharged" obelin the luminescent protein from the calcium concentration in the double logarithmic coordinates (figure 2).

Example 4.1. In the in vitro system, at room temperature, 230 ál sample, in which the need to determine the concentration of calcium, made of 20 μl of a solution containing 5 x 10-5M photoprotein obelin the luminescent protein, 20 mm TrisHCl buffer, or PIPES, pH 7. Mix thoroughly for 1 min Under continuous irradiation of light to measure the intensity of the fluorescence excitation spectrum at the wavelengths of excitation and reception, which were used to build the calibration graph. Consider the logarithm of the fluorescence intensity. The resulting value of the logarithm in which ensenaste substituted in the calibration graph (the dependence of the fluorescence intensity in the spectrum of the excitation discharged obelin the luminescent protein from the calcium concentration in the double logarithmic coordinates) and find the corresponding the value of the logarithm of the calcium concentration. To determine the molar concentration of calcium is necessary to build ten to the power value of the logarithm of the calcium concentration, was found in a calibration curve for obelin the luminescent protein.

Example 4.2. In the system of in vivo administered 0.1 mg coelenterazine per 1 g of cells. Under continuous irradiation of light to measure the intensity of the fluorescence excitation spectrum at the wavelengths of excitation and reception, which were used to build the calibration graph. Consider the logarithm of the fluorescence intensity. The resulting value of the logarithm of the intensity substituted in the calibration graph (the dependence of the fluorescence intensity in the spectrum of the excitation discharged obelin the luminescent protein from the calcium concentration in the double logarithmic coordinates) and find the corresponding value of the logarithm of the calcium concentration. To determine the molar concentration of calcium is necessary to build ten to the power value of the logarithm of the calcium concentration, was found in a calibration curve for obelin the luminescent protein.

Example 5. Preparation of calibration graph for the determination of calcium concentration using fetoprotein aquarina. In the in vitro system, at room temperature, 230 μl of an aqueous solution of calcium at different concentrations (5·10-9M, 10-8M, 5 x 10-8M, 10-7M, 5 x 10-7 M, 10-6M, 5 x 10-6M, 10-5M, 10-4M, 10-3M) add 20 μl of a solution containing 5 x 10-5M fetoprotein aquarina, 20 mm TrisHCl buffer, or PIPES, pH 7. In each case, the solution is stirred thoroughly for 1 min to fully conduct bioluminescent reaction, which forms a fluorescent product called discharged photoprotein acorin. Fluorescence analysis was carried out under continuous irradiation of a solution of light. The intensity of the fluorescence excitation spectrum discharged aquarina measured at a wavelength of excitation in the range of 320-350 nm and registration on the wavelength in the range 440-530 nm. Construct a calibration graph of fluorescence intensity in the spectrum of the excitation Sa-discharged" Aquarena on the concentration of calcium in the double logarithmic coordinates.

Example 5.1. In the in vitro system, at room temperature, 230 ál sample, in which the need to determine the concentration of calcium, made of 20 μl of a solution containing 5 x 10-5M fetoprotein aquarina, 20 mm TrisHCl buffer, or PIPES, pH 7. Mix thoroughly for 1 min Under continuous irradiation of light to measure the intensity of the fluorescence excitation spectrum at the wavelengths of excitation and reception, which were used to build the calibration graph. Consider the logarithm intense is vnesti fluorescence. The resulting value of the logarithm of the intensity substituted in the calibration graph (the dependence of the fluorescence intensity in the spectrum of the excitation discharged aquarina on the concentration of calcium in the double logarithmic coordinates) and find the corresponding value of the logarithm of the calcium concentration. To determine the molar concentration of calcium is necessary to build ten to the power value of the logarithm of the calcium concentration, was found in a calibration curve for aquaria.

Example 5.2. In the system of in vivo administered 0.1 mg coelenterazine per 1 g of cells. Under continuous irradiation of light to measure the intensity of the fluorescence excitation spectrum at the wavelengths of excitation and reception, which were used to build the calibration graph. Consider the logarithm of the fluorescence intensity. The resulting value of the logarithm of the intensity substituted in the calibration graph (the dependence of the fluorescence intensity in the spectrum of the excitation discharged aquarina on the concentration of calcium in the double logarithmic coordinates) and find the corresponding value of the logarithm of the calcium concentration. To determine the molar concentration of calcium is necessary to build ten to the power value of the logarithm of the calcium concentration, was found in a calibration curve for aquaria.

Example 6. Post eenie calibration curve to determine the concentration of calcium using fetoprotein litina. In the in vitro system, at room temperature, 230 μl of an aqueous solution of calcium at different concentrations (5·10-9M, 10-8M, 5 x 10-8M, 10-7M, 5 x 10-7M, 10-6M, 5 x 10-6M, 10-5M, 10-4M, 10-3M) add 20 μl of a solution containing 5 x 10-5M fetoprotein litina, 20 mm TrisHCl buffer, or PIPES, pH 7. In each case, the solution is stirred thoroughly for 1 min to fully conduct bioluminescent reaction, which forms a fluorescent product called discharged photoprotein Klitin. Fluorescence analysis was carried out under continuous irradiation of a solution of light. The intensity of the fluorescence excitation spectrum discharged litina measured at the wavelength of excitation, which is included in the range of 320-350 nm and registration on the wavelength included in the range 400-500 nm. Construct a calibration graph of fluorescence intensity in the spectrum of the excitation Sa-discharged" litina on the concentration of calcium in the double logarithmic coordinates.

Example 6.1. In the in vitro system, at room temperature, 230 ál sample, in which the need to determine the concentration of calcium, made of 20 μl of a solution containing 5 x 10-5M fetoprotein litina, 20 mm TrisHCl buffer, or PIPES, pH 7. Mix thoroughly for 1 min Under continuous light irradiation measure intensives the ü fluorescence excitation spectrum at the wavelengths of excitation and reception, which were used to build the calibration graph. Consider the logarithm of the fluorescence intensity. The resulting value of the logarithm of the intensity substituted in the calibration graph (the dependence of the fluorescence intensity in the spectrum of the excitation discharged litina on the concentration of calcium in the double logarithmic coordinates) and find the corresponding value of the logarithm of the calcium concentration. To determine the molar concentration of calcium is necessary to build ten to the power value of the logarithm of the calcium concentration, was found in a calibration curve for litina.

Example 6.2. In the system of in vivo administered 0.1 mg coelenterazine per 1 g of cells. Under continuous irradiation of light to measure the intensity of the fluorescence excitation spectrum at the wavelengths of excitation and reception, which were used to build the calibration graph. Consider the logarithm of the fluorescence intensity. The resulting value of the logarithm of the intensity substituted in the calibration graph (the dependence of the fluorescence intensity in the spectrum of the excitation discharged litina on the concentration of calcium in the double logarithmic coordinates) and find the corresponding value of the logarithm of the calcium concentration. To determine the molar concentration of calcium is necessary to build ten to the power of testing the value of the logarithm of the calcium concentration, found via a calibration curve for litina.

The advantages of the proposed method are:

- in opportunities for continuous use initially introduced indicator for biochemical process of any duration in living cells;

a greater signal intensity;

- photoluminescence easier to check, since measurements are not connected with the conduct of additional biochemical processes;

and there is the ability to use fluorescent reporter calcium in combination with bioluminescent analysis.

Fluorescence method for determination of calcium concentration on the basis of the discharged photoproteins, including bioluminescent reaction fetoprotein and calcium ions, the measurement of the fluorescence intensity of the solution, the construction of gauge dependence of fluorescence intensity on the concentration of calcium in the double logarithmic coordinates and determine the value of the logarithm of the calcium concentration on the logarithm of the measured intensity of the fluorescence, wherein fluorescence of the trigger light source after completion of the bioluminescent reaction and measure the intensity of fluorescence at a given wavelength excitation and reception.



 

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FIELD: medicine.

SUBSTANCE: system comprises a biological signal measurement unit, an electroencephalogram interface unit for discriminating a user's request and identifying a function which is linked to the user's request, an analysis unit for measuring and the analysing a physical value, an event-related potential waveform storage unit, a user's characteristic extraction unit and a setup unit. A procedure consists in setting up a discrimination method in the electroencephalogram interface unit and provides measuring a visual and/or acoustical stimulation related physical value to be accepted as a characteristic value of stimulation. It is followed with finding variations of the characteristic value of stimulation which has an effect on the event-related potential. The event-related potential waveform is saved, and a user's characteristic value is extracted while being based on the saved event-related potential waveform. A random-access memory stores a computer program and activates a computer to follow the steps of said setup procedure.

EFFECT: simplified calibration, more precise identification of electroencephalogram and high-manipulation interface.

16 cl, 15 dwg

FIELD: medicine.

SUBSTANCE: duration, amplitude and form of each continuous brain signal wave are matched with parameters of each sensory signal. Recording each continuous brain signal wave, forming a matched sensory signal and exposing a patient to this signal are consistent and immediate. During the procedure, the patient is suggested to pay attention that the sensory signals perceived reflect brain work.

EFFECT: method enables more efficient process normalising psychophysiological state following stress load, psychoemotional and mental strain, and while treating functional disorders of central nervous system, psychosomatic diseases and consequences of organic cerebral affections.

4 cl

FIELD: medicine.

SUBSTANCE: duration, amplitude and form of each continuous brain signal wave are matched with parameters of each sensory signal. Recording each continuous brain signal wave, forming a matched sensory signal and exposing a patient to this signal are consistent and immediate. During the procedure, the patient is suggested to pay attention that the sensory signals perceived reflect brain work.

EFFECT: method enables more efficient process normalising psychophysiological state following stress load, psychoemotional and mental strain, and while treating functional disorders of central nervous system, psychosomatic diseases and consequences of organic cerebral affections.

4 cl

FIELD: medicine.

SUBSTANCE: surgery is preceded with carrying out a spectral analysis of cardiac rhythm variability and specifying the VLF, LF, HF values. If the ratio VLF:LF:HF is 35.5:25.8:38.7%, an unfavourable postoperative course is predicted, while the ratio VLF:LF:HF being 25.3:34.9:39.8% states a favourable postoperative course to be expected.

EFFECT: method extends the range of products for predicting the postoperative course in the patients underwent rhinosurgeries.

3 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: continuous electrocardiosignal is recorded, filtered and presented as discrete records. In all derivations, the discrete records are summed over modulus. A threshold level is formed to be compared to a value of the sum over modulus of the discrete records of electrocardiosignal. A cardicycle, QRS-complex electrocardiosignal and R-R interval are extracted. In the extracted cardiocycle, values of derivatives Y' of the discrete records of electrocardiosignal are calculated. It is followed with extracting an interval of the values of an electrocardiosignal derivative belonging to the QRS-complex electrocardiosignal with the beginning and termination of the extracted interval of the values of the electrocardiosignal derivative are the beginning and termination of the QRS-complex electrocardiosignal.

EFFECT: more precise extraction of the beginning and termination of the QRS-complex.

3 cl, 11 dwg

FIELD: medicine.

SUBSTANCE: continuous electrocardiosignal is recorded, filtered and presented as discrete records. In all derivations, the discrete records are summed over modulus. A threshold level is formed to be compared to a value of the sum over modulus of the discrete records of electrocardiosignal. A cardicycle, QRS-complex electrocardiosignal and R-R interval are extracted. In the extracted cardiocycle, values of derivatives Y' of the discrete records of electrocardiosignal are calculated. It is followed with extracting an interval of the values of an electrocardiosignal derivative belonging to the QRS-complex electrocardiosignal with the beginning and termination of the extracted interval of the values of the electrocardiosignal derivative are the beginning and termination of the QRS-complex electrocardiosignal.

EFFECT: more precise extraction of the beginning and termination of the QRS-complex.

3 cl, 11 dwg

FIELD: medicine.

SUBSTANCE: continuous electrocardiosignal is recorded, filtered and presented as discrete records. In all derivations, the discrete records are summed over modulus. A threshold level is formed to be compared to a value of the sum over modulus of the discrete records of electrocardiosignal. A cardicycle, QRS-complex electrocardiosignal and R-R interval are extracted. In the extracted cardiocycle, values of derivatives Y' of the discrete records of electrocardiosignal are calculated. It is followed with extracting an interval of the values of an electrocardiosignal derivative belonging to the QRS-complex electrocardiosignal with the beginning and termination of the extracted interval of the values of the electrocardiosignal derivative are the beginning and termination of the QRS-complex electrocardiosignal.

EFFECT: more precise extraction of the beginning and termination of the QRS-complex.

3 cl, 11 dwg

FIELD: medicine.

SUBSTANCE: method involves carrying out ultrasonic scanning examination of subclavian artery over its whole extent in physiological arm position with arterial blood pressure being measured in the middle one third of the arm. Next, when applying compression tests, blood circulation parameters variations are recorded in distal segment of the subclavian artery with arterial blood pressure being concurrently measured. Three degrees of superior thorax aperture syndrome severity are diagnosed depending on reduction of linear blood circulation velocity and arterial blood pressure compared to their initial values. Mild one takes place when linear blood circulation velocity reduction reaches 40% and arterial blood pressure 20% of initial level, moderate one when linear blood circulation velocity reduction reaches 70% and arterial blood pressure 50% and heavy one when linear blood circulation velocity reduction is greater than 70% of initial level and arterial blood pressure is greater than 50% to the extent of no blood circulation manifestation being observed in the subclavian artery.

EFFECT: high accuracy of diagnosis.

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