Method of differential diagnostics of bullous dermatoses

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to dermatovenerology. In order to carry out differential diagnostics of bullous dermatoses level of blister formation in skin is determined. Erosion on skin is processed with acridine orange in dilution 1:5000 on physiological solution and examined by means of frontal lens of contact 60 fold magnification objective. If blister bottom is represented by cells of granular epidermis layer, the cells being large, horizontally oriented with nuclei of various size, configuration and light intensity with cytoplasm in granular layer cells having yellowish glow and nuclei with signs of lysis, disintegration of epidermis cells is detected, in blister cavity there are acantolytic cells with large nuclei and glowing red cytoplasm, seborrheal pemphigus is diagnosed. If bottom of erosion is represented by cells of basal layer, and in cells there are large nuclei with intensive green glow, acantolytic cells have a large nucleus and intensively glowing red cytoplasm and when lens of objective touches the erosion bottom disintegration of epidermis cells is detected and its strata with acantolytic cells are freely floating in tissue fluid, pemphigus vulgaris is diagnosed. If destruction of basal membrane is observed, blister bottom is represented by papillary layer of derma, cell infiltrate, where non-fluorescent dark papillary is detected capillaries are contouring, Duhring's dermatitis herpetiformis is diagnosed.

EFFECT: method ensures high accuracy of diagnostics at early stages of bullous dermatites during short period of time.

7 dwg, 3 ex

 

The invention relates to medicine, namely to dermatology, may be applied in a comprehensive diagnosis of bullous dermatoses. Differential diagnosis of bullous dermatosis is one of the urgent problems of dermatology. Thus an important diagnostic signs are histopathological patterns, the sum of the changes in the epidermis and dermis, characteristic of a particular study [1]. The differential diagnosis of the cystic dermatosis very important point is the establishment of a level bubble formation in the skin. In particular, when acantholycosa the bladderwort (AP), toxic bullosa (TEN), the syndrome of burned skin (SSSS), some forms multiform exudative erythema (MEA), in which the bubbles are located in the epidermis. While the bottom of the bladder may be a layer of granular, subulate or basal cells (AP, TEN or SSSS). In other forms of MAA, the syndrome of Stevens-Johnson herpetiformis dermatosis during the bubble, as a rule, is the subepidermal nature [1].

Modern morphological methods for diagnosis of bullous dermatosis associated with the improvement primarily immunomorphological methods studies showing levels of fixation Ig G, rarely Ig And in the epidermis or in the zone of the basal membrane of the skin. When using the Western blot turns, you can viewitem skin "targets" for autoantibodies (desmoglein 3 and 1, when the disease, the proteins of the basal membrane BP 180 and 320 when the bullous pemphigoid etc) [2, 3].

Immunomorphological research methods are quite complicated to implement, require invasive skin biopsy with subsequent sample preparation and processing of the slices labeled fluorescein antibodies. Given that bullous dermatitis are sometimes aggressive and heavy character development in a relatively short time, threatening the life of the patient, the diagnostic questions require more than a quick solution. Therefore, it seems appropriate to search for new approaches to the diagnosis of bullous dermatoses.

The known method of epiluminescence microscopy (ELM) skin. It is non-invasive and allows clinically to assess fully - increasing oil impression - morphological features of the skin, not visible to the naked eye. ELM can be done handheld portable microscope, equipped with colourless lens, which allows you to receive a fixed 10-fold amplification. Incident light at an acute angle of 20 get from the built-in light source, equipped with a battery in the unit.

Immersion oil covers the entire damaged area and provides not only easy sliding, but also eliminates surface reflection due to the different refractive in the EU air and skin. This makes the outer layer is transparent, allowing the visualization of pigmented anatomical structures of the epidermis in vivo, as well as the underlying layers, dermoepidermal transition, which is impossible in the study of the naked eye [2]. The disadvantage of this method is low resolution capability of the lens, which does not allow to verify the cells of the epidermis and, consequently, the level of occurrence of the bubble in the skin. Closest to the proposed method is a method of differential diagnosis of bullous dermatoses, including the determination of the level of bubble formation in the skin, while erosion on the skin is treated with acridine orange at a dilution of 1:5000 physiological solution, and then conduct research using the front lens contact lens 60-fold increase. The method allows to determine the location of the bubbles, ie - sabcomeline, intraepidermal (adbaseline) and subepidermal, as well as the presence acantholytic cells, eosinophils, leukocytes, lymphocytes and plasma cells. Moreover, it is known that one of the diagnostic signs is easy disintegration of the cells of the epidermis tapping the lens of bottom erosion. (http://www.worklib.ru/laws/ussr/10005432.php.)

The disadvantage of this method are the diagnostic signs, including the formation of the bubble, with the composition of the cellular infiltrate (acantholycosa cells, eosinophils, lymphocytes) and easy disintegration of the cells of the epidermis. These signs can be observed with the vulgar, and the seborrheic disease. And when the dermatitis of visible only during dedifferentiate layers of the epidermis.

The use of contact microscopes creates new perspectives of research in biology, medicine, as it allows to study not only the fine cell structure and its change in living cells and tissues, but also allows for monitoring of the physiological functions of living bodies flowing in their physiological, pathological and transport processes [4] (figure 1).

Object of the invention is the efficiency of differentiation bullous dermatoses.

The task to solve due to the fact that, if the bottom of the bubble is represented by the cells of the granular layer of the epidermis, the cells are large, horizontally oriented, with nuclei of different size, configuration and intensity of illumination, while in cells of the granular layer of the cytoplasm has a yellowish glow, and kernel - signs of lysis marked disintegration of the cells of the epidermis, in the cavity of the bubble - acantholycosa cells with large nuclei and glowing red cytoplasm, diagnose seborrheic the bladderwort;

if the bottom erosion is represented by the basal layer cells, and the cells are large nuclei with intense who ate green glow acantholycosa cells have a large nucleus and intensely glowing red cytoplasm, and when you touch the lens on the bottom erosion marked disintegration of the cells of the epidermis and layers it with contritions cells swim freely in the tissue fluid, diagnose vulgar disease;

if you see the destruction of the basal membrane and the bottom of the bubble presents the papillary layer of the dermis, defined cellular infiltration, where contribut effluorescence dark papillary capillaries, diagnosed with dermatitis herpetiformis during the.

The method is as follows. Erosion on the skin is treated with acridine orange (AO) in a dilution of 1:5000 physiological solution. After 10 minutes in the erosion enter the front lens contact lens 60-fold increase, and conduct microscopic examination of the studied surface. Contact biomicroscopy (KB) is conducted at the facility, which is based on optical block part LUMAM AND 3, mounted on the rod that can bring contact lenses to any part of the body. Diagnosis carried out on the determination of the depth of bubble formation in the skin. When vulgar disease can be seen that the bottom of the bubble is represented by the basal layer cells, and the cells are large nuclei with intense green luminescence, centrifically have a large nucleus and intensively SwetsWise red cytoplasm, and when you touch the lens on the bottom erosion marked disintegration of the cells of the epidermis and layers it with contritions cells swim freely in the tissue fluid. When seborrheic the bladderwort bottom of the bubble is represented by the cells of the granular layer of the epidermis, the cells are large, horizontally oriented, with nuclei of different size, configuration and intensity of illumination, while in cells of the granular layer of the cytoplasm has a yellowish glow, and kernel - signs of lysis marked disintegration of the cells of the epidermis, in the cavity of the bladder acantholycosa cells with large nuclei and glowing red cytoplasm. When herpetiformis dermatitis of the see during the destruction of the basal membrane and the bottom of the bubble presents the papillary layer of the dermis, defined cellular infiltration, where contribut effluorescence dark papillary capillaries. The research time per person averaged 20 minutes.

Results were examined in 14 patients with bullous dermatoses, on the basis of Regional dermatovenerologic dispensary (CCVD). Using KB identified 7 patients vulgar bladderwort (EAP), 2 patients with seborrheic disease and 5 patients with dermatitis herpetiformis during the (child friendly city). The research was conducted and tires bladder, and directly erosive morphological elements. The diagnosis was established on the basis of CL the technical picture, and confirmed by histological examination method. When KB skin healthy individuals without lesions were seen private Horny scales, the illumination of which is homogeneous, the kernel was not determined.

A similar pattern was observed when the CB surface (tires) bubble. And when KB in patients with seborrheic hand, foot on the place revealed bullous elements, the microscopic picture depended on the depth of their location and the nature of the bottom of the bladder (figure 2).

When seborrheic the bladderwort bottom of the bubble was represented by the cells of the granular layer of the epidermis. Cells were large, horizontally oriented, with nuclei of different size, configuration and intensity of the glow. In the cells of the granular layer of the cytoplasm had a yellowish glow, and the engine had signs of lysis (deformation, fragmentation, disintegration of cells of the epidermis, and in the cavity of the bladder acantholycosa cells with large nuclei and glowing red cytoplasm (table). The table shows that when using the KB in the prototype signs vulgar and seborrheic bladderworts are the same: the presence of acantholytic cells and easy disintegration of the cells of the epidermis.

In patients with vulgar disease in KB on the proposed method, the bottom of the bubble was represented by the cells of the basal layer cells was noted large nuclei the intense green glow cells bore the signs of disintegration. Acantholycosa cells had a large nucleus and intensely glowing red cytoplasm, when you touch the lens on the bottom erosion was observed disintegration of cells of the epidermis and layers it with contritions cells has floated freely in the tissue fluid. When using the contact lens with 60-fold magnification and diluted in physiological solution acridine orange was at the bottom of the erosion of differentiated cells infiltration (Fig 3, 4, 5, 6).

In patients with herpetiformis dermatitits of during when KB was observed destruction of the basal membrane (subepidermal location of the bubble). The bottom of the bubble was presented to the papillary layer of the dermis, where it was determined the cellular infiltrate was konturirovany effluorescence dark papillary capillaries (7), (table).

As can be seen from the table, when using KB (prototype), we see only acantholycosa cells and easy disintegration of the epidermis, but to differentiate vulgar or seborrheic disease is impossible, dedifferentiate layers of the epidermis with dermatitis of during that very little for diagnosis. In our proposed method, contact biomicroscopy (KB) we verificarem the cells of the granular, spinous and basal layers of the epidermis, defined acantholycosa cells, thereby differentiate Volga the ing, seborrhoeic the disease and dermatitis herpetiformis during the.

Thus, the proposed method is fast, efficient and allows to differentiate bullous dermatoses in the early stages.

Example 1. Patient R. 72 years, turned in CCVD Krasnoyarsk with complaints about the appearance of rashes on the skin and mucous membranes of the oral cavity. The first rash appeared on the mucous membrane of the oral cavity. After 6 months the rash spread over the skin surface.

Local status: Process is widespread, with predominant localization on the mucosa of the oral cavity, the skin of the trunk, upper and lower extremities. The rash presents the mucosa of the oral cavity multiple erosions. On the skin - bubbles with a loose lid and erosion. Symptom of St. Nicholas and the symptom of Asbo-Hansen positive. In connection with intolerance patients lidocaine diagnostic skin biopsy was not possible. Was held contact biomicroscopy erosion bladder. When KB revealed the following characteristics: bottom erosion is represented by the basal layer cells, and the cells are large nuclei with intense green glow acantholycosa cells have a large nucleus and intensely glowing red cytoplasm, and when you touch the lens on the bottom erosion marked disintegration of the cells of the epidermis and layers it with acanto oticheskimi cells swim freely in the tissue fluid. Data morphological features characteristic of vulgar bladderworts. The patient was prescribed the appropriate treatment.

Example 2. Patient K., 65 years old, came in CCVD with complaints of itching of the skin and the appearance of bubbles. Rash appeared 6 days ago.

Local status: Process is widespread and symmetrical in nature, with localization on the skin in areas of the extensor surfaces of the limbs. Lesions are papules, blisters, erosions and serous-hemorrhagic crusts. Items are grouped. Symptom Nicholas negative. Was held contact biomicroscopy erosion bladder. When KB is revealed: the destruction of the basal membrane, the bottom of the bubble presents the papillary layer of the dermis, defined cellular infiltration, where contribut effluorescence dark papillary capillaries. Listed morphological features characteristic of dermatitis herpetiformis during the. The patient was prescribed the appropriate treatment.

Example 3. Patient L., 62, was admitted to the hospital CCVD with widespread skin lesions. For the first time the rash appeared 1 month ago, was treated by a dermatologist with a diagnosis of drug reaction, without effect. Last week, the condition worsened, the process spread.

Local status: common process, with localization on the skin of the back, chest, hairy frequent the head. The process is represented by: bubbles 1-2 cm in diameter, erosions and serous crusts of yellow color. The symptom positive Nikolsky. For the differential diagnosis of the patient was conducted KB erosion bladder. The bottom of the bubble is represented by the cells of the granular layer of the epidermis, the cells are large, horizontally oriented, with nuclei of different size, configuration and intensity of illumination, while in cells of the granular layer of the cytoplasm has a yellowish glow, and the engine shows signs of lysis marked disintegration of the cells of the epidermis, in the cavity of the bubble - acantholycosa cells with large nuclei and glowing red cytoplasm. Data morphological features characteristic of seborrheic bladderworts. The patient was assigned to appropriate therapy.

Literature

1. Liever''s histopathology of the skin. Lippinkoft Raren. New York.l997. - 1073 p.

2. Kolpakov FI, I. prokhorenkov Contact microscopy of the skin in dermatological practice. // VDV. - 1985. No. 4. - P.18-19.

3. Barsky IA, Polyakova NI, Jakubenas A.V. Contact microscopy. - M. 1976. "Medicine".

4. Elchingen. L.V., I. prokhorenkov Contact biomicroscopy and microfluorimetry skin in the diagnosis of dermatoses. The methodological. REC. the assertion. The Ministry of health, USSR, 1987. - P.3.

The method of differential diagnosis of bullous dermatoses, including the determination of the level of bubble formation in the skin, the less the ku erosion on the skin acridine orange at a dilution of 1:5000 physiological solution, conducting research using the front lens contact lens 60-fold increase, characterized in that, if the bottom of the bubble is represented by the cells of the granular layer of the epidermis, the cells of a large horizontally oriented with nuclei of different size, configuration and intensity of illumination, while in cells of the granular layer of the cytoplasm has a yellowish glow, and the engine shows signs of lysis marked disintegration of the cells of the epidermis, in the cavity of the bubble - acantholycosa cells with large nuclei and glowing red cytoplasm, diagnose seborrheic the disease; if the bottom erosion is represented by the basal layer cells, and the cells are large nuclei with intense green glow acantholycosa cells have a large nucleus and intensely glowing red cytoplasm, and when you touch the lens on the bottom erosion marked disintegration of the cells of the epidermis, and layers it with contritions cells swim freely in the tissue fluid, diagnose vulgar disease; if you see the destruction of the basal membrane and the bottom of the bubble presents the papillary layer of the dermis, defined cellular infiltration, where contribut effluorescence dark papillary capillaries, diagnosed with dermatitis herpetiformis during the.



 

Same patents:

FIELD: machine building.

SUBSTANCE: test cell consists of internal cylinder with inside installed piston moving axially owing to hydraulic facility for regulation of pressure and volume of fluid medium; it is installed on the side of the piston for investigated fluid medium. Also the said internal cylinder has a thin wall and is closed from the said side for investigated fluid medium. The cell has an external cylinder arranged coaxially outside the said internal cylinder, thus creating circular space between the said cylinders. Notably, the external cylinder has a reinforced and sturdy structure and at least one orifice for inlet of hydraulic fluid medium to a hydraulic side of the said piston opposite to the said side for investigation of fluid medium and to the said circular space. Further, circular space has less radial dimension, than maximal range of flexible expansion of the said internal cylinder; therefore, break of the said internal cylinder because of pressure drop on the wall of the cylinder is impossible due to restriction with the said external cylinder.

EFFECT: safe study of pressure, volume and temperature and avoiding break and deformation of internal cylinder.

3 cl, 1 dwg

FIELD: chemistry.

SUBSTANCE: several large sources of pollutants of river water are selected on the map of a city or some other populated place. Monitoring sites are marked before, in the middle and after each source. The first monitoring site is installed beyond the boundary of the city or some other populated place and the last monitoring site is beyond the boundary of that city or some other populated place. From the first monitoring site, growing distance to the next monitoring sites lying on the boundary on large sources of pollutants along the river bank is measured, as well as before and after the city or some other populated place, and after analysing the river water samples for concentration of pollutants and evaluation of conformity of the measurement results with the maximum allowable concentration, measurement results are evaluated along the river bank depending on the growing distance from the first monitoring site to the next monitoring site and taking river water samples.

EFFECT: broader functionalities of measuring pollution of large rivers with waste water and higher accuracy of associating these measurement results to river bank area.

8 cl, 7 dwg

Immersion probe // 2405132

FIELD: physics.

SUBSTANCE: immersion probe has a housing whose longitudinal axis is surrounded by an outer surface. Part of the outer surface of the probe is fitted with sampling elements which pass around the longitudinal axis in the peripheral direction and which have radial extension. Around the sampling elements there is a protective layer which can dissolve or burn in molten steel or slag. The sampling elements have a flat or concave surface facing away from the immersed end of the housing of the probe. The sampling elements can also have a flat or concave surface which faces away from the immersed end and which is inclined relative the longitudinal axis in the direction of the end of the housing of the probe, opposite the immersed end. The sampling elements are in form of one or more annular segments lying one behind the other in the direction of the longitudinal axis.

EFFECT: efficient collection of slag samples.

15 cl, 4 dwg

FIELD: oil and gas industry.

SUBSTANCE: method for determining oil-and-gas saturated intervals involves removal of slurry and gas diluted in drill fluid, analysis of that gas and relation of gas logging to certain depths. At that, slurry is removed during well drilling. Oil hydrocarbons are extracted from slurry samples. Well-logging curves are built for hydrocarbons C5+ highest. Connection of hydrocarbon logging is performed as per anomaly of hydrocarbon compound at header - not header boundary.

EFFECT: improving accuracy of prompt extraction of producing intervals of headway when drilling deep oil and gas wells.

2 cl, 2 dwg, 5 tbl

FIELD: medicine.

SUBSTANCE: system comprises a sampling and detecting unit enclosed in a protective cover with a touch opening and accommodating a gas analyser spaced from the opening and generating an alarm when recording vapours of the controllable substances, a movable body having a contact pad exposed in the finger touch opening, a system for transferring the contact pad of the movable body to the gas analyser, a sensor activating a scanning cycle, a fingerprint reader arranged opposite to the contact pad, a desorption chamber hydraulically connected to the gas analyser, a cooling area, a control unit containing a device for fingerprint database storage and comparison to reference fingerprints, a person's identification indicator. In addition, the system accommodates a gas passage with its one end connected to an input of the gas analyser, and its other end - to a finger seat on the fingerprint reader. The movable body has an aperture wherein the contact pad closing the aperture and removable therefrom with a finger on the fingerprint reader, and a resilient member used to return the contact pad in a position to close the aperture of the movable body are fixed.

EFFECT: application of the presented system allows to detect the trace amounts of volatile substances, to improve reliability of fingerprint identification.

3 cl, 3 dwg

FIELD: medicine.

SUBSTANCE: differential diagnostics of herpetic infections of gastric mucosa us membranes of a stomach or dental pulp is ensured by coordination marking of a biopsy material. Histologic sections of thickness no more than 3 mcm are prepared. The prepared sections are applied on slides as follows: the first section is arranged on the first slide, the second section is applied on the second, the third slide is used for the third section, the fourth section is placed on the first slide and further the same sequence, the number of slides is determined by the identified virus count. Each preparation is analysed for specific viruses of the herpes groups by immunohistochemistry with using specific monoclonal antibodies. The results of each reaction are recorded by the presence of specifically stained antigen granules in a histologic sample, and the images of the results of each reaction are saved in the electronic form. The formed images of the following sections are aligned by the coordination mark. If observing the specifically stained antigen granules of various localisation, herpetic mixed infection of gastric mucosa and dental pulp is diagnosed. When observing the specifically stained antigen granules of the same localisation herpetic monoinfection with immune cross-reaction is diagnosed.

EFFECT: technique presents higher accuracy of diagnosing herpetic infections of gastric mucosa or dental pulp ensured by differential diagnostics between mixed infection and monoinfection with immune cross-reaction.

3 dwg, 3 ex

FIELD: physics.

SUBSTANCE: sample collection device has housing with end sealing, a sampling tube with a shutoff cock and a flexible reinforced hose with a ferromagnetic tip and a permanent magnet. A hollow casing made from diamagnetic or paramagnetic material is placed vertically and air-tightly from the bottom to the top in the container and inside the said case on the entire height of the container there is a linear permanent magnet motor mounted to a motor scanning inductor through lining made from magnetically permeable material and capable of vertical displacement on a fixed secondary element of the electric motor together with its inductor and stopping at the required point of the secondary element. The ferromagnetic tip of the flexible hose, held in such a position by magnetic attraction force, is constantly located opposite the permanent magnet outside the casing.

EFFECT: interval-wise collection of liquid and gas from a container.

2 cl, 1 dwg

Sampling unit // 2399723

FIELD: mining.

SUBSTANCE: sampling unit includes glass holder, removable sampling sleeve, and stock equipped with a handle. Sampling sleeve is provided with possibility of being fixed on the stock end, made from thin flexible spring steel and cut along the generatrix. The end of sampling sleeve is provided with L-shaped slots, and inner surface of glass holder is provided with projection made so that it can interact with the above slot.

EFFECT: maintaining inherent characteristics of soil sample.

3 dwg

FIELD: instrument making.

SUBSTANCE: device comprises dosing syringe. Nozzle made of body and pipeline of blowing gas supply is installed at the end of syringe body. Device also comprises sealing elements, providing together with nuts for tight connections of nozzle body with syringe body and its stem. Nozzle body includes cylindrical cavity, where blowing gas is supplied along pipeline. Diametre of this cavity is more than diametre of syringe piston.

EFFECT: improved quality of chromatographic analysis and reliable operation of device.

2 dwg

FIELD: machine building.

SUBSTANCE: equal proportions of material from running flow are periodically cut off for samples in specified and equal time intervals; also equal amount of material is cut off to sample from all parts of flow at cross and straight cross section of flow and its equal thickness. Amount of sampled material is proportional thickness of flow at different flow thickness. In the beginning running flow of loose material is directed along trajectory of free fall. Further, it is crossed with a divider along its whole width and thickness in cross section. Its cross part is cut off, and material is simultaneously removed with the divider in a lengthwise direction along whole thickness of flow. Also material is mixed, there is formed a flow of material with specified parametres and its qualitative characteristics are determined. The facility consists of a sampler installed in the flow of loose material on a frame and travelling by means of a chain drive, of a driving gear connected to an electric motor by means of a reducer, and of a driven rotary screw installed in a case of the sampler; also the case has upper loading and lower un-loading slots. The loading slot of the sampler case has length equal or bigger, than thickness of falling flow of loose material, while the screw with an alternated step from the upper loading to the lower unloading slot rotates at constant speed around its proper axis and in the direction of lengthwise section of the falling flow of loose material. An unloading trapdoor is installed under a receiving conveyor; a material spreader is positioned above continuous moving surface of the conveyor.

EFFECT: raised reliability of loose material samples facilitating application of instruments for continuous control of material quality.

4 cl, 3 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to phthisiology, and can be used in TB clinics for formation of groups of higher risk of disease recurrence among cured patients with newly diagnosed infiltrative pulmonary tuberculosis for the moment of main treatment course end. Presence of risk factors of tuberculosis process progressing in remote period of observation is determined: group A - interruption of main treatment course, widespread infiltration for the moment of treatment beginning - 3 segments and more, drug resistance, contact with patient with open form of tuberculosis before beginning of treatment; group B - men, cavities of decay with 2 cm and more diametre at the beginning of treatment, duration of course treatment not higher than 180 doses, violation of regulated period of preventive fluoroexamination before detection of disease, interruption of hospital treatment. Presence of prognostically significant qualitative factors of type of residual post-tuberculosis changes (RTC) in remote period of observation is determined: contact with patient with open form of tuberculosis (x1), social status (x3), use of drugs (x4), completeness of hospital stage (x5), cavity of decay (x6), completeness of course treatment (x9), residual post-tuberculosis changes at the moment of detection (x10), massiveness of bacteria release (x12), in case if quality factor is absent it is equaled to 0, if it is present - to 1. Presence of prognostically significant quantitative factors of type of residual post-tuberculosis changes in remote period of observation is determined: number of affected lung segments (x2), age - years (x7), duration of course treatment - doses (x8), duration of intensive phase - doses (x11). After that, prognosis of "minor" (Y1) and "major" (Y2) RTC in remote period of observation are determined by formulas Y1 and Y2. On the basis of Y1 and Y2 values comparison conclusion about prediction of "minor" and "major" RTC is made. In case if one risk factor of tuberculosis progressing in remote period from group A or two and more from group B are absent, and 'minor" RTC are present, low degree of recurrence risk, requiring observation in III group of specialised dispensary registration for 1 year, is determined, if one factor of group A or two and more factors of group B are present and presence of "major" RTC, high degree of recurrence risk, requiring observation in III group of specialised dispensary registration for 3 years, is determined, if one factor of group A or two and more factors of group B are present and presence of "minor" RTC, medium degree of recurrence risk, requiring observation in III group of specialised dispensary registration for 2 years, is determined, and if, if one risk factor from group A or two and more from group B are absent, and presence of "major" RTC, medium degree of recurrence risk, requiring observation in III group of specialised dispensary registration for 2 years, is determined.

EFFECT: method allows to reduce frequency of recurrences due to improvement of specialised dispensary observation in group III of specialised dispensary observation of cured patients with newly diagnosed infiltrative pulmonary tuberculosis as the most frequent form of the disease.

4 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, in particular to dentistry, and is intended for sampling biopsy material from mucous membrane of prosthetic bed for orthopedic rehabilitation of patients with post-operative defects of jaws with dental prostheses. Mucosa is excised in one section by mucotome with 3.0 mm diametre. Each following sampling is carried out with lap on the previous one in such way that diametre of section of mucosa affection after carrying out four analyses constitutes 6.0 mm. Sampling of biopsy material is performed in terms, 24 hours, 5 days, 14 days and 28 days.

EFFECT: method makes it possible to carry out sampling of biopsy material in one section in different terms of post-operative wound epithelisation.

1 dwg

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to surgical dentistry and is intended for determination of degree of damage to inferior alveolar nerve in dental implantation. Carried out is measurement of electroexitability (EOD) of tooth pulp on corresponding side of lower jaw and electric potentials (EP) of face skin three times in each of 4 points: in area of mouth corner, chin, in projection of mental and inferior dental foramen of lower jaw, by means of apparatus PARKELL 0624. After that average index of EP is calculated: N=n1+n2+n3+n4/4, where n1, n2, n3, n4 are average values of face skin EP in each of 4 points. If values of teeth EOD indices are 12-25 mcA and face skin EP 45-55 mcA, temporary cessation of nerve conduction, indicating a mild degree of damage is diagnosed; if teeth EOD is 26-50 mcA and face skin EP is 55-80 mcA - injury of nerve segment resulting from compression by dental implant is diagnosed, if teeth EOD is 51-100 mcA and face skin EP is 80-150 mcA, complete cessation of nerve trunk conduction is diagnosed.

EFFECT: method allows to establish trauma nature, determine pathogenetic treatment and thus accelerate process of patients' rehabilitation.

1 dwg, 3 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to obstetrics and otolaryngology. Claimed method lies in the following: in order to predict protracted course of acute rhinofaryngitis in case of influenza A(H3N2) in the second trimester of pregnancy, in women content of antiviral bodies in pair blood serums is determined, as parametre, first serum (A) is used, concentration of middle-molecule peptides (units of optic density) (B). degree of expression of nasal mucosa edema during period of disease height in points (C); after that prediction of development of protracted course of acute rhinopharyngitis by means of discriminant equation: D=-0.099A-112.032B+2.654C, where D is discriminamt function with boundary value equal 25.48; if D is equal or less than boundary value, development of protracted course of acute rhinopharyngitis in case of influenza A(H3N2) in women in the second trimester of pregnancy, and if D is higher than boundary value, absence of protracted course of acute rhinopharyngitis in case of influenza A(H3N2) in women in the second trimester of pregnancy. Probability of correct prediction constitutes 85.9%.

EFFECT: ensuring prediction of protracted course of acute rhinopharyngitis in case of influenza A(H3N2) in women in the second trimester of pregnancy.

2 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to clinical immunology and allergology, and can be used in pediatrics for diagnostics of bronchial asthma in children of early age. Method includes analysis of anamnesis data, estimation of clinical symptoms, as well as studying of allergologic status, which is carried out taking into account index of eosinophils in general blood test and level of general immunoglobulin E in blood serum. Additionally carried out is analysis of induced sputum with determination of eosinophil percentage in it. If said index is higher or equal 5%, it is considered to be main proof for diagnosis "bronchial asthma".

EFFECT: method allows for the first time to establish diagnosis of disease in children under 5 years old from group of risk on bronchial asthma development.

4 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to internal diseases, diagnostics. Method is based on determination of general oxidant activity (GOA) and general antioxidant activity (GAA) with further determination of oxidant index (OI), which equals ratio of GOA to GAA. In accordance with the invention oxidant activity is determined by degree of echinochrome A oxidation by components of oxidant system of blood serum or plasma, and general antioxidant activity is determined by degree of echinochrome A oxidation with chloramine B, added to blood serum or plasma. OI index of healthy donor is taken as a unit. OI value higher than a unit testifies to disbalance of general oxidant status of organism. Method ensures up to 20 fold reduction of amount of analysed serum or plasma in comparison with standard methodology, reduction for carrying out analysis from 72 hours to 2 hours. When ranging it is possible to perform analysis of more than 500 samples during one day, using microboards and microplate spectrophotometers.

EFFECT: method is simple in implementation, economical and does not require large volume of thermostatically controlled chambers.

1 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to neuroinfections. In children older than 11 years old, determined are presence of retrobulbar neuritis (XI), foci of white and gray substance of brain and spinal marrow CNC (X2), in blood: progesterone in nmol/k (X3), luteinising hormone (LH) in MU/l (X4), folliculostimulating hormone (FSH) in MU/l 1 (X5), testosterone in nmol/l (X6), estradiol in pkmol/l (X7). By obtained values discriminant function,

is calculated. If Y values are lower than 0.5, favourable outcome is predicted, if Y values are higher than 0.5 - development of disseminated sclerosis is predicted.

EFFECT: method increases accuracy of predicting disseminated sclerosis development, which is achieved due to application of prognostically significant indices and their complex estimation.

3 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, in particular to physiology, biochemistry and can be used for express diagnostics of organism adaptation to hypokinesia both in usual conditions, and during space flights. Blood parametres are determined in patients only with hypokinesia and with hypokinesia at the background of desmopressin introduction, obtained results are compared and conclusion is made, as parametres mass-spectral determination of proteomic profiles of blood serum in mass range from 1000 to 17000 with processing of samples with magnetic particles MB WCX and MB IMAC Cu is carried out, and if there is no change in signal intensity after introduction of desmopressin with pre-fractioning by magnetic particles MB IMAC Cu m/z=9133 in comparison with initial data, as well as with reliable reduction of peak intensity m/z=1297, m/z=2769, m/z=7764, m/z=6432, m/z-6630 when pre-fractioning with magnetic particles MB WCX in these users stage of organism adaptation to hypokinesia with introduction of desmopressin is diagnosed.

EFFECT: method acceleration, possibility to determine about 20 parametres during one analysis, minimal amount of biomaterial (5-10 mcl), as well as possibility of said technique automation.

5 cl, 3 tbl, 1 dwg

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to therapy and gastroenterology. Clinical symptoms and risk factors are determined from life anamnesis: presence of dry cough, presence in anamnesis of chronic obstructive lung disease, presence in anamnesis of bronchial asthma, regular intake of broncholytic medications, presence of tickling in throat, presence in anamnesis of chronic diseases of ENT organs (laryngitis, pharyngitis, sinusitis, recurring otitis media), expression of cardialgia, regular intake of beta-blockers, regular intake of calcium antagonists, remotedness of endured myocardial ischemic attack. Their gradations and numerical values are determined, after which prognostic coefficients are determined. On the basis of comparison of prognostic coefficients values, risk of unfavourable course of gastroesofageal reflux disease is predicted.

EFFECT: method allows to increase accuracy of prediction.

1 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to otorhinolaryngology. Before surgery in patient's nasal cavity type of skull construction is determined by head index. If it corresponds to brachycephaly, hemorrhage from posterior parts of nasal cavity is predicted; in case of dolichocephaly - hemorrhage from anterior parts of nasal cavity or ethmoidal labyrinth, and in case of mesocephaly - possibility of hemorrhage from both anterior and posterior parts of nasal cavity.

EFFECT: method allows to reduce frequency and expression of intra- and post-operative nasal hemorrhages.

2 dwg, 1 ex

FIELD: medicine, clinical toxicology.

SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.

EFFECT: higher accuracy of prediction.

2 ex, 3 tbl

Up!