Method for prediction of gastric ulcer cicatrisation rate
SUBSTANCE: invention refers to medicine, particularly to gastroenterology and therapy, can be used to update a prediction of clinical course of the gastric ulcer (GU). It involves the evaluation of an ulcer defect, Helicobacter infection content, and hemolytic resistance parametres. An individual dynamics of ulcer defect cicatrisation of a wall of stomach is calculated by formula: Cicatrisation dynamics = 0.139831+0.853145·S_prior - 0.0000181334· tlat-0.0221184· Kmax - 0.000185502·HP, where S_prior is the defect area prior to the initiation of treatment, Kmax is a maximum erythrocyte hemolysis rate constant, tlat is latent hemolysis time, HP is the Helicobacter infection content. And if the derived coefficient is equal to or less than 0.6, ulcer healing within a therapeutic standard time limit is predicted, if it exceeds 0.6, prolonged gastric ulcer healing is supposed.
EFFECT: method allows to estimate the ulcer defect cicatrisation dynamics even prior to the initiation of treatment that in turn allows to optimise drug therapy.
1 tbl, 1 dwg
The invention relates to medicine, in particular to a gastroenterologist and therapy, can be used to Refine the prognosis of gastric ulcer (GU).
There is a METHOD of PREDICTING the COURSE of GASTRIC ULCER AND DUODENAL ULCER (patent No. 2246731 (RU) from 2005.02.20). The authors conduct a diagnostic study, with 5-6 days from the beginning of exacerbation of peptic ulcer of stomach and duodenum determine the content of serotonin, histamine and acetylcholine in the blood, then in 2-3 weeks conduct drug therapy and again determine the level of serotonin, histamine and acetylcholine in the blood and when the content of serotonin in 2-3 times above the norm, histamine 1.15-1.4 times higher than normal, and acetylcholine - 20-45% below normal forecast for gastric ulcer and duodenal ulcer as not cicatrizing ulcers.
The disadvantages of the method is the high cost due to the use of specific reagents and equipment for determining serotonin, histamine and acetylcholine.
There is a METHOD of PREDICTING the course of GASTRIC ULCERS AND DUODENAL ULCERS ASSOCIATED WITH HELICOBACTER PYLORI (patent No. 2269132 (RU) from 2006.01.27). The essence of the method is that carried out the biochemical analysis of blood, determine the titer of antibodies to Helicobacter pylori class IgG and IgA, and when the title to the each of them is 1:200 and more forecast for gastric ulcer and duodenal ulcer, associated with Helicobacter pylori, with exacerbations. The technical result is to increase the accuracy of the prognosis of the disease.
The disadvantages of the method: diagnosis and prediction is done using only one clinical symptom - titer antibodies to Helicobacter pylori, not taking into account the peculiarities and possibilities of their own systems of an organism of the patient.
Closest to the claimed method is a method of DIAGNOSING PATHOLOGIES of the GASTROINTESTINAL TRACT of the PATIENT (patent No. 2098819 (RU) from 1997.12.10). The method includes determining the phase currents of the gastro-intestinal tract, including peptic ulcer disease, and assessment of individual sensitivity of the patient to the development of peptic ulcer. The patient carry out blood collection, separation and its control sample in saline to a reference concentration of red blood cells, the effect on samples with hydrochloric acid, followed by determining at set intervals of values of optical density study and control samples, which make the conclusion about the percentage concentration of red blood cells, separated by a characteristic group resistance to the hemolytic effects of hydrochloric acid: value added, demoted-, rednisone, and it is judged on the condition of the gastrointestinal tract, before exposure to blood samples with hydrochloric acid to produce their incubation with lekti the AMI in doses threshold concentration, not cause agglutination of red blood cells, and the condition of the gastrointestinal tract of the patient is judged as follows: increase percent concentration povsemestnij erythrocytes indicates early signs of ulcer and erosive lesions of the mucous membrane, the increase in the percentage concentration pangenetic erythrocytes indicates remission of the disease.
The drawbacks of the invention: the complexity of calculating portion for identifying group affiliation resistance of erythrocytes; no justification of the boundaries of the partition coefficients in groups of resistance.
The purpose of the invention is development of a method of predicting the course of GU, allowing to assess the dynamics of cicatrization of the ulcer before treatment, which, in turn, will allow to optimize drug therapy.
This goal is achieved by the fact that at the preliminary stage conducted a study of the osmotic properties of red blood cells obtained from the blood of patients with SUD. We have studied clinical and laboratory dynamics of the disease in 12 patients with SUD. Used automatic registration method acid and osmotic erythrogram on the installation, with the following blocks:
1) optical (photoelectrocolorimeter FEC-56M with built-in differential amplifier or CPK-2MP);
2) recording (two-Regis is combined with LCD-003 and digital voltmeter type B7-20);
3) thermostatic (ultraharmonic UTU-6).
Hemolysis of erythrocytes was performed in a thermostatted cells with outer dimensions of 20×40×10 mm and a working volume of 4 ml measurement of the values of transmittance was carried out at a wavelength of λ=490 nm, since in this region of the spectrum, the molar extinction coefficient of aqueous solutions of oxyhemoglobin minimum. Thus, the application of a spectrophotometric method for recording the intensity of the parallel light fluxes that have passed through the control and working of the cell, allows you to test not only the release of hemoglobin into the environment of incubation, and the intensity of light scattering suspensions of erythrocytes, the value of which is proportional to the volume of erythrocyte cells and the number of them in the environment.
When conducting hemolysis of red blood cells in one cell installation registers the S-shaped cumulative curve whose shape reflects the total change of the magnitude of light scattering (τ %) in the studied solution in time.
The registration method of the kinetic curves allows to assess the structural condition of erythrocytes by the following parameters:
tlatthe latent time period of hemolysis (s, min);
t50the time half of hemolysis (s, min);
Tomax- constant maximum rate of hemolysis (Rel. units);
GSFthe relative number of spherocytes (%);
G - Rel the relative number of hemolyzed cells (%).
The kinetics of the acid hemolysis was detected after adding in the working cell with 5 ml of the suspension of erythrocytes 100 μl of acid (Hcl 0.1 N.). The choice of Hcl due to its stability during storage, and the presence of both ions (H+and CL-in plasma.
At admission and after standard treatment for SUD compared the dynamics of scarring ulcers of the stomach wall, the duration of treatment, hemoglobin, presence of Helicobacter pylori (HP).
A fragment of the table containing the studied parameters below.
|The performance of some patients with FBG|
|tlat(s)||Kmax(atmospheric air)||b (g/l)||HP||The size of the ulcer (cm)||Bed-day|
|before the treatment||after the treatment|
|320||3,2||118||+||0,6 x 0,8||0||16|
|180||3,40||135||+||of 0.5 and 0.6||0||17|
|195||of 2.51||140||+++||1,5×1,8||of 0.5 and 0.6||30|
|173||2.42||143||1,5×1,9||0,6 x 0,6||32|
The main parameter characterizing the resistance of cells to acid, is the value of tlatthe time latentes the period of hemolysis, the value of Kmax- constant describing the maximum rate of hemolysis of erythrocytes.
We calculated the dependence of the dynamics of scarring ulcers, defined as the difference of the square of the defect before treatment and after treatment has been studied clinical and laboratory criteria based on multiple regression analysis. It was found that the greatest contribution to the dynamics of scarring make settings such as the area of the defect before treatment (S_), the constant maximum speed of hemolysis of erythrocytes (Kmax), the latent time period of hemolysis (tlat), infection of Helicobacter (HP).
The regression equation is as follows:
Further mathematical processing showed that the duration of treatment is statistically significant relationships with those calculated using equation coefficient dynamics of scarring (see drawing). Moreover, the average values of this coefficient is calculated for a group of patients who have had complete scarring, 0.6; for a group of patients who have had partial scarring, and 2.1, the differences between groups on this topic were statistically significant. As a practical guidance on the use of our method can be applied in dagiti the following algorithm individual forecast speed scarring ulcers: if the ratio of dynamics, calculated by the proposed formula, 0.6 or less, the healing of ulcers will be provided in the standard treatment time, if the ratio is more than 0.6, expected long-term healing of stomach ulcers.
Thus, the proposed formula for calculating the coefficient of the dynamics of scarring using the area of the ulcer of the stomach wall before treatment, a constant maximum rate of hemolysis of erythrocytes, the latent time period of hemolysis and the degree of H. pylori colonization is easy to use, does not require the Clinician complex calculations, allows to reliably differentiate each patient individually according to the intensity of ulcer healing. For this differentiation, in turn, will allow the doctor to prescribe the best course of treatment, standard or enhanced, will reduce the time and increase the effectiveness of treatment.
A method for predicting the course of gastric ulcer, including determining the size of the ulcer, colonization of H. pylori infection, the parameters of resistance to hemolytic effect, characterized in that the dynamics of individual cicatrization of the ulcer of the stomach wall calculated by the formula: Dynamics rubs.=0,139831+0,853145·S_ - 0,0000181334·tlat- 0,0221184·Kmax- 0,000185502·HP
where S_ - the area of the defect is cured before the I; Kmax- constant maximum rate of hemolysis of erythrocytes; tlatthe latent time period of hemolysis; HP - infection of H. pylori infection, if the ratio is equal to or less than 0.6, predict healing of ulcers in the stipulated standard of treatment time, if more than 0.6 - expected long-term healing of stomach ulcers.
SUBSTANCE: DNA is recovered from patient's peripheral venous blood to detect TNFα gene polymorphism. If observing -308GA and -308AA genotypes, a severe course of chronic calculous cholecystitis with frequent alternating exacerbations is predicted. -308GG genotype detected enables predicting moderate and mild courses of chronic calculous cholecystitis of recurrent or monotonous pattern.
EFFECT: use of the method allows to predict the clinical course and severity of chronic calculous cholecystitis in a relatively short time.
1 tbl, 1 ex
SUBSTANCE: integrated examination for Cytomegalovirus, Chlamydophila pneumoniae, Chlamydophila psittaci, Legionella pneumophila, Moraxella catarrhalis detection involves the PCR-based DNA recovery from four mixed biological substrata, including sputum, bronchoalveolar lavage, posterior pharyngeal and epipharyngeal wall smear, saliva of 3-5 patients (children and adults).
EFFECT: reduced probability of false-negative results as compared to analysis of only one of them and substantially more profit-proved than the detailed analysis of all the substrata separately.
SUBSTANCE: initial blood and a food extract solution are prepared and mixed. When mixing, the food extract solution is added in the amount 4-12 vl % and incubated in constant agitation. A thermally untreated natural food homogenate is used as an initial food. When preparing thereof, at first a mother solution is prepared and analysed for protein concentration, e.g. by Bradford protein assay. Then it is dissolved to the use rate by an antioxidant solution, e.g. phenol, a process solution is prepared in physiologic saline. A dissolution criterion is specified by such protein concentration that corresponds to base active protein concentration in the initial food.
EFFECT: higher information value of the samples, high reliability and accuracy of the analysis results to follow.
5 cl, 1 ex
SUBSTANCE: fertile window is determined in the menstrual cycle by saliva electrolyte composition test ensured by electrolytic current generated by potassium ion diffusion in the saliva. That is ensured by the pre-evaluation of electrolytic current in the absence of the fertile window, thereafter this value is used as a reference to be matched with the relevant present electrolytic current when determining fertile window time and length. The fertile window time is determined by increasing electrolytic current as compared with the same value in the absence of the fertile window, and the fertile window length is described by the increasing time of the corresponding electrolytic current.
EFFECT: higher accuracy of determination of the fertile window time and length.
3 dwg, 3 ex
SUBSTANCE: method for biological modelling of staphylococcal allergy involves the sensitisation of cavies with staphylococci suspension 5.0-10.0 bln/ml deactivated by autoclaving and detoxicated in 0.2% formalin, and sorbed on aluminium hydroxide 3-5 mg/ml. The suspension prepared as described above is single subcutaneously introduced to cavies in the amount 0.5-1.0 ml per one animal. The allergic reaction is provoked by the intracutaneous introduction of 0.1 ml of staphylococcal allergen dissolved 1:10, 1:20 and 1:40. The reaction is estimated by skin hyperemia 24 hours later.
EFFECT: permanent allergy simulation in laboratory animals.
SUBSTANCE: assessment of local inflammation activity with underlying uterine cervical diseases involves the collection of biomaterial samples to be analysed by delivering a native material sampling agent to the external orifice of uterus to a nidus surface directly without introducing a sampling agent inside the cervical canal. The collected material is fully immersed in a test tube filled with 1.0 ml of 0.155 M saline solution and agitated in a vortex centrifuge for 10 minutes. The prepared materials are investigated by enzyme-linked immunosorbent assay (ELISA) and analysed for cytokine interleukin-1β concentration by spectrophotometric method. The derived value within 70 pg/ml to 150 pg/ml enables to observe the presence of a chronic inflammatory process, while the value exceeding 150 pg/ml shows the presence of an active inflammatory process and expressing immune response.
EFFECT: method allows eliminating injuries of the mucosa and improving the quality of the investigated native material without complicating the investigation process.
3 cl, 3 ex
SUBSTANCE: analysed sample is illuminated with coherent radiation and a field of vision is selected in the beam of radiation passing through the analysed sample using an aperture diaphragm which enables to view a fragment of the speckle image of mutual interference of coherent waves with random phase shift and random set of intensities as a result of scattering of light on particles of the analysed sample. The size of the field of vision is such that 1-4 speckles fall in it. Coefficient of variation of intensity of illumination of the field of vision with time is then determined, from the change of which aggregation capacity of particles and behaviour of aggregation is determined.
EFFECT: higher rate, accuracy and sensitivity of analysing aggregation capacity.
3 cl, 5 dwg, 1 tbl, 5 ex
SUBSTANCE: in animals in erythrocytes and renal tissue concentration of malone dialdehyde (MDA) and activity of Na+, K+-ATP-ase of renal tissue are determined simultaneously. If MDA values are 4.88±0.03 nmole/ml of erythrocital mass and higher, and in cells of cortical and medullary renal tissue respectively 2.18±0.03 and 3.57±0.06 nmol/ml of protein and higher and values of activity of Na+, K+-ATP-ase of cortical and medullary renal tissue 2.21±0.08 and 5.39±0.13 mcmol "Рн" /mg protein/hour and lower respectively, nephropathy is diagnosed. In diagnostics of nickel nephropathy by said method taken into account are disorders of renal water- and electrolyte-excretory function, as well as pathogenic mechanisms of said disorders.
EFFECT: possibility to apply method in analysis of pathogenic mechanisms of toxic action of heavy and non-ferrous metals, in particular nickel, on functional state of kidneys.
4 tbl, 1 ex
SUBSTANCE: there is claimed method of diagnosing atrophic process in vagina in women after radical operations on organs of small pelvis. Immunogystochemical analysis of vaginal biopsy material is carried out with calculation of number and diametre of vessels in them, for which purpose biopsy of vagina in its middle third with sampling of biopsy material of size 0.5x0.5x0.3 cm is performed, after that, biopsy material is fixed in neutral 10% formalin for 18 hours with further passing in isopropanole and pouring into paraffin, after that staining of deparaffinated cuts with hematoxylin and eosin is carried out in accordance with classical methods with preliminary disclosing of antigen in citrate buffers for CD34, result of reaction is identified by nuclear cell staining, number and size of vessels, visualised by means of CD34, and in case of vessels of medium and large diametre with area 3-10 mm2 are present, norm is determined, and if vessels with area 2 mm2 and less are present, vaginal atrophy is diagnosed.
EFFECT: increase of diagnostics efficiency.
2 ex, 5 tbl
SUBSTANCE: in newborn children, who are in critical state, on the first day of life in a single urine sample, cholinesterase activity is determined (per 1 mg of excreted with urine creatinine) and if value of cholinesterase activity is equal 10 mU/mg creatinine or higher, severe ischemic nephropathy is diagnosed, when clinical manifestations of ischemic nephropathy are hidden by anatomical and physiological peculiarities of a newborn baby.
EFFECT: possibility to apply method for early diagnostics of severe ischemic nephropathy in full-term newborn babies in critical state, in early neonatal period.
SUBSTANCE: invention refers to medicine, namely phthisiology, and can be used for prediction of development of respiratory tuberculosis.
EFFECT: method provides more effective genetic prediction of respiratory tuberculosis in adults ensured by PCR-based molecular HLA - DQB1* gene typing, and if detecting HLA - DQB1*05 allele in the genotype, the risk of development of respiratory tuberculosis and unfavourable course are predicted.
1 tbl, 1 dwg, 1 ex
SUBSTANCE: invention refers to medicine, namely to urology. To establish the indications for prostate biopsy, prostate secretion is analysed. Kallikrein (KK) activity (mcmol x min-1 x l-1) and angiotensin converting enzyme (ACE) activity (mcmol x min-1 x l-1) are evaluated. Provided 18.20≤KK≤58.62 and 32.16≤ACE≤51.20, prostate biopsy is considered to be indicated.
EFFECT: method provides higher reliability of establishing the indications for prostate biopsy, is easy to implement, and does not require considerable material costs.
SUBSTANCE: invention refers to medicine, namely to intraluminal endoscopic surgery of stomach and duodenum, and can be used in treating the patients with giant gastric and duodenal ulcers. An ulcer surface is exposed to endoscopic therapy by punctual noncontact surface monopolar coagulation with a centripetal argon plasma flame at flow rate 2.2 l/min starting in a 'SPRAY' mode at flow intensity 44 Wt, exposure length 2-3 seconds for each point. After a 30-second pause, the ulcer surface is treated with the argon plasma flame in a 'COAG' mode at flow intensity 44 Wt, exposure length 2-3 seconds for each point. 5 days later, the 'SPRAY' mode treatment of the ulcer surface is applied once more at flow intensity 40 Wt, exposure length 2-3 seconds for each point. If after 7 days following the second treatment, the monitoring fiberoptic gastroduodenoscopy shows a maximum ulcer diametre exceeding 1.5 cm, the third 'SPRAY' mode ulcer treatment is required at flow intensity 40 Wt, exposure length 2-3 seconds for each point. General coagulation time for the entire ulcer surface area in each treatment session makes 20-30 seconds.
EFFECT: method allows better treatment optimisation and improved conservative therapeutic effect in giant gastric and duodenal ulcers, as well as prevention of potential complications development.
SUBSTANCE: invention relates to field of medicine, namely to surgery. Blood is sampled from cubital vein in patients with acute pancreatitis on 1-3 and 7-10 day from disease beginning. Level of blood serum myoglobin is determined by method of performing reaction of passive hemagglutination. If level of blood serum myoglobin on 1-3 day increase from 95 to 128 ng/ml, acute fatty pancreatic necrosis is diagnosed, if myoglobin level in blood serum is higher than 128 ng/ml, hemorrhagic pancreatic necrosis is diagnosed, and if level of myoglobin in blood serum on 7-10 day increases higher than 256 ng/ml, hemorrhagic pancreatic necrosis in stage of infection of pancreatic necrosis nidus is diagnosed.
EFFECT: method allows to correct drug therapy and individually ground tactics of treating patients with acute pancreatitis depending on severity of disease form and presence of infection.
1 tbl, 3 ex
SUBSTANCE: invention relates to field of medicine, namely to diagnostic methods. Homogenate of placenta of pregnant woman who had herpes-virus infection is analysed by ELISA method for activity of granzyme B, titre of antibodies in pregnant woman's peripheral blood, and simultaneously number of syncyotrophoblast nuclei in apoptosis is calculated. If antibody titre is 1:3200 and granzyme B content is 283.14±18.00 pg/ml, percentage of nuclei in apoptosis state is 1.5±0.09%; if antibody titre is 1:6400 and granzyme B content in placenta homogenate is 712.84±23.00 pg/ml content of nuclei in apoptosis state is 2.0±0.12%; if titre of antibodies to herpes virus is 12800 and content of granzyme B in placenta homogenate is 1007.53±31.50 pg/ml (control - 251.10±31,00 pg/ml) percentage of nuclei in state of apoptosis increases to 3.5±0.08% (control - 1.0±0.009%).
EFFECT: method ensures high sensitivity of detecting nuclei in state of apoptosis in syncyotrophoblast at the background of increased content of granzyme B in placenta homogenate.
SUBSTANCE: invention relates to field of medicine, namely to laboratory analysis and can be used for prediction of course of wound process in case of purulent-inflammatory diseases if maxillofacial region of microbial etiology. In carrying out microbiological analysis of wound discharge, presence or absence of microorganisms and their concentration are determined, in case of immunologic analysis registered are total number of leukocytes, ESR, levels of medium circulating immune complexes (CIC), antibodies to streptococcus (ASL-O), immunoglobulin A (IgA). Analysis is carried out befire and on 5-7 day after operation, and in case of complete elimination of microorganisms in wound or reduction of their concentration to <103 CFU/tamp., as well as reduction in blood serum for not less than 30% of total number of leukocytes, antibodies to streptococcus, and reduction for not less than 25% of value of ESR, CIC, IgA, favourable course of wound process is predicted, in case if indices are different - prediction is unfavourable.
EFFECT: method allows to reduce time of patient's hospitalisation, carry out adequate therapy in post-operational period in due time, does not require additional equipment for carrying out microbiological and immunological analysis.
SUBSTANCE: invention relates to field of medicine, in particular to cardiology, neurology, ophthalmology, is used to carry out diagnostics of predisposition to ischemic neuroopticopathy in elderly people with ischemic heart disease (IHD), arterial hypertension (AH) and dyscirculatory encephalopathy (DEP). Blood is sampled from ulnar vein, blood samples are forms, from which DNS is separated, amplification of DNA fragments is pertformed, after that genotyping on polymorphisms del-425 and 3'-UTR of c-fms geneand polymorphism 64V of CCR2 gene is carried out, if in elderly women heterozygous genotype 64V/641 of polymorphism 64V of CCR2 gene, and heterozygous genotype 12 of polymorphism 3'-UTR of c-fms gene are detected, predisposition to ischemic neuroopticopathy is diagnosed.
EFFECT: detection of predisposition to ischemic neuroopticopathy in elderly people for administration of adequate preventive treatment.
7 tbl, 2 ex
SUBSTANCE: invention relates to medicine, namely to biochemical investigations in oncology, and can be used in determination of activation or stabilisation of pathologic process in patients with primary-resistant Hodgkin's lymphoma. In patients with primary-resistant Hodgkin's lymphoma at stages of treatment, by radioimmune method determined is content in blood of thyroid gland hormones - triiodothyronine and thyroxine, and adrenocortical hormone - cortisol, their concentration is summed up and in case of total level of hormones is within 278.2 nmole/l - 0 333.0 nmole/l conclusion about activation of malignant process is made, if total level of said hormones is 432.4 nmole/l - 833.2 nmole/l conclusion about absence of process activation is made.
EFFECT: prediction of process activation or stabilisation in patients with primary-resistant Hodgkin's lymphoma is an informative test for evaluation of pathologic process state and can be recommended for monitoring of patients at treatment stages.
1 tbl, 2 ex
SUBSTANCE: invention relates to medicine, namely to obstetrics, and can be used for prediction of preterm effusion of amniotic fluid in pregnant women with gestation term 32-36 weeks. Essence of invention lies in the following: in blood serum of pregnant women with gestation term 32-36 weeks IL-8 level is determined, and if its value equals or is lower than 15.4 ng/ml, preterm effusion of amniotic fluid is predicted.
EFFECT: method is simple in implementation and allows to predict development of preterm effusion of amniotic fluid with high accuracy, sensitivity and specificity, which makes it possible to select correct tactics of monitoring women with risk of said obstetrics pathology development, to avoid complications of labour activity and perinatal pathology.
1 tbl, 4 ex
SUBSTANCE: invention relates to field of medicine, namely to diagnostic methods and deals with method of diagnosing entry of nuclei of placenta pili syncyotrophoblast into apoptosis by determining in placenta homogenate content of IgG and IgM antibodies to phosphatidylserine in pregnant women, who had herpes-virus infection in the third trimester of pregnancy. Method lies in determination of IgG and IgM antibodies to phosphatidylserine in placenta homogenate by ELISA method. If titre of antibodies to HSV-1 equalled 1:6400, 4.70±0.08 U/ml of IgG and IgM antibodies to phosphatidylserine were detected; if titre was 1:12800, content of IgG and IgM antibodies to phosphatidylserine increased to 5.60±0.06 U/ml (control - 2.50±0.07 U/ml), number of syncyotrophoblast nuclei in state of apoptosis constituted 4.50±0.20% (control - 1.5±0.20%).
EFFECT: method possesses high sensitivity and allows to predict development of apoptosis in nuclei of placenta pili syncyotrophoblast in case if number of IgG and IgM antibodies to phosphatidylserine in placenta grows.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl