Method for establishing indications for prostate biopsy
SUBSTANCE: invention refers to medicine, namely to urology. To establish the indications for prostate biopsy, prostate secretion is analysed. Kallikrein (KK) activity (mcmol x min-1 x l-1) and angiotensin converting enzyme (ACE) activity (mcmol x min-1 x l-1) are evaluated. Provided 18.20≤KK≤58.62 and 32.16≤ACE≤51.20, prostate biopsy is considered to be indicated.
EFFECT: method provides higher reliability of establishing the indications for prostate biopsy, is easy to implement, and does not require considerable material costs.
The invention relates to medicine, namely to urology, and will be used to determine the indications for biopsy of the prostate.
Development of methods for determining the indications for biopsy of the prostate gland, suitable for mass screening, is one of the main tasks of modern urology.
In the United States every year, which amounts to 300 thousand new cases of prostate cancer (PC). 30 thousand men per year in the US die from this disease. The problem of determining the indications for prostate biopsy is extremely relevant and in Russia, where every year, which amounts to about 20,000 new cases of PC. The incidence is 25.6 per 100 thousand population. An annual growth rate of 63.9% (Bealestreet, Kamniska. Hormonal therapy of patients with prostate cancer. J. "Oncourology", 2007-2008, M., special issue, pp.2-5).
There are many ways to define the indications for prostate biopsy. The modern way to define the indications for prostate biopsy based on the joint application definition prostatespecific antigen (PSA) and digital rectal exam (IF), in conjunction with transrectal ultrasound (transrectal us) prostate. The combination of these techniques biopsy of the prostate is shown in 80% of patients. But overall RUSNANO, that DOG standard, prostatespecific the marker, interspecifically to 20% (Pannek J., Partin A.W., Prostate-specific antigen: whats new in 1997. Oncology (Williston Park) 1997; 11: 1273-82). Diagnostic value indicators such as the ratio of free PSA to total, the rate of increase of PSA, density and range of normative age-related PSA values, is under study.
Sensitivity is not higher than 50%. Many urologists find this research is justified only for technical support biopsy.
Prostate biopsy is the only reliable tool for the detection of an existing prostate cancer (Chibichan MB, Kogan M.I. Phenomenon of migration stage prostate cancer after radical prostatectomy. J. "Bulletin of the southern scientific center, Rostov-on-don, 2009, Volume 5, No. 1, p.11-17). However, even with 10-12 point biopsy of the prostate 10-35% of men with negative initial biopsy have cancer at repeat biopsy. Urologists have to repeat uncomfortable for patients with prostate biopsy, accompanied by 25% of patients with severe pain, hematospermia, hematuria (Djavan B, et al. J Urol 2000; 163:1144-9; Roehl KA, et al. J Urol 2002; 167:2435-9); Heidenreich A, et al. EurUrol 2008; 53:68-80 (EAU guidelines on prostate cancer 2008).
Although biopsy remains the "gold standard" for detection of prostate cancer VC is SHL, for making decisions about the need for biopsy requires the development of new methods for determining the indications for biopsy of the prostate.
Conducted research on the medical-scientific and patent literature found different ways to determine the indications for biopsy of the prostate.
The study I.Rehman, A.R.Azzouzi, J.W.F.Catto, S.Alien, S.S.Cross, K.Feely, M.Meuth, F.C.Hamdy Proteomic analysis of voided urine after prostatic massage from patients with prostate cancer: a pilot study. Urology 64:1238-1243, 2004, describes how to determine the indications for biopsy of the prostate gland, where patients with suspected FPW investigated urine after massage of the prostate gland. Urine was centrifuged to remove cells and debris, spent separation and protein markers by two-dimensional polyacrylamide gel electrophoresis and identified protein markers. Detection in urine protein Calgranulin B/MRP-14 biopsy of the prostate was considered shown.
In the same study described another way to define the indications for prostate biopsy, where a similar method was investigated urine of the patient, and in the presence of protein in it Transferrin A chain (36 kDa) biopsy of the prostate was considered shown.
The disadvantages of the above methods is the low specificity of the markers, the complexity of the technological platforms: the need for special equipment the equipment and staff of specially trained staff, lack of reliability.
The closest technical solution adopted by the us as a prototype, is how to define the indications for prostate biopsy described Bussemakers MJG, et al. (Cancer Res 1999; 59:5975-9), US Patent No. 5185439 at which conduct the study of the biological material in the third portion of urine after massage of the prostate gland. The method is based on the technology of nucleic acid amplification, used for detecting mRNA Prostate specific gene 3 (Prostate Cancer Gene 3 (RS)) in the urine samples of men. Urine is treated by adding a Transport Medium for Urine (SCI), which is the lysis of cells and stabilization of RNA. Next, the analysis counts RSA and PSA mRNA ratio RA/PSA mRNA define the index RSA. When the index PCA 3 more 100 - biopsy of the prostate is considered shown.
The disadvantage of this method of determining the indications for prostate biopsy is its complexity, high cost of research, which hampers the possibility of large-scale implementation in practice, the lack of accuracy is 89%.
The aim of the present invention is to simplify the method of determining the indications for biopsy of the prostate, reducing the cost of its implementation, improving the reliability determination by the Azania biopsy of the prostate.
The objective is achieved by determining in a biological fluid of the patient - the secret of the prostate gland activity (µmol × min-1× l-1) kallikrein (KK) and activity (µmol × min-1× l-1) angiotensin-converting enzyme (ACE)inhibitors. Under the conditions 18,20≤QC≤58,62 and 32,16≤ACE≤51,20 biopsy of the prostate is considered shown.
The proposed method of determining the indications for prostate biopsy is as follows.
The patient after the massage the prostate gland taking the secret of the prostate gland, which determine the activity (µmol × min-1× l-1) kallikrein (KK) and activity (µmol × min-1× l1) angiotensin-converting enzyme (ACE)inhibitors.
Before the study the secret of the prostate gland is diluted in the ratio of 1:9 with saline to a volume of at least 0.5 ml
The activity of kallikrein determined by a spectrophotometric method for rate of hydrolysis of N-benzoyl-1-arginine ethyl ester (BAEE) (Pashina T.S., Krinsky AV Simplified method for the determination of kallikrein and kallikrein serum (plasma) blood in norm and in certain pathological conditions. Questions of medical chemistry. 1974; 20(6): 660-663).
The activity of kallikrein is expressed in (µmol × min-1× l
The activity of angiotensin-converting enzyme (ACE) is determined by a spectrophotometric method using a synthetic substrate ACE forilalililla-glycylglycine (BASSOON) (Golikov P.P., Nikolaev POSTGRADUATE rapid method for determining the activity of angiotensin-converting enzyme in serum // Klin. Lab. Diagnosis. - 1998. - 1. - C.11-13).
Enzyme activity is expressed as µmol × min-1× l-1.
Practical feasibility of the proposed method of determining the indications for prostate biopsy confirmed an example from clinical practice.
The patient badgers S.L., 59 years old, was admitted to outpatient evaluation at the clinic of urology of the Rostov Gosmeduniversiteta (Rostov state medical University) with a preliminary diagnosis of prostatic hyperplasia.
Complaints: frequent and painful, difficulty urinating. The history of the disease: these complaints symptoms occur within 2 years.
The results has shown that the boundaries of the prostate fuzzy; iron is moderately enlarged, hardened, non-uniform.
Evaluation of urinary international scale assessment of prostatic symptoms (IPPS) - 18 points.
Urinalysis is normal. Laboratory diagnosis: PSA=9,426 ng/ml. the Results of transrectal us prostate cancer: volume issue the ATA - 26,3 cm3; residual urine volume - 70 cm3.
To determine the indications for prostate biopsy the patient study was conducted according to the claimed method.
The patient after a massage of the prostate was carried out by the fence secret of the prostate gland, which determined the activity concentration of kallikrein (KK) and the activity of angiotensin-converting enzyme (ACE).
Before the study obtained the secret of a prostate cancer patient was diluted in the ratio of 1:9 with saline to a volume of 0.5 ml.
The activity of kallikrein (KK) was determined spectrophotometrically by the method of the speed of hydrolysis of N-benzoyl-1-arginine ethyl ester (BAEE) (Pashina T.S., Krinsky AV Simplified method for the determination of kallikrein and kallikrein serum (plasma) blood in norm and in certain pathological conditions. Questions of medical chemistry 1974; 20(6):660-663).
The activity of kallikrein was expressed in µmol × min-1× l-1. It was obtained the value of QC=34,48 mmol × min-1× l-1.
The activity of angiotensin-converting enzyme (ACE) inhibitors was determined by a spectrophotometric method using a synthetic substrate ACE forilalililla-glycylglycine (FAGG) (Golikov P.P., Nikolaev POSTGRADUATE rapid method for determining the activity of any antiprogressive enzyme in serum // Klin. Lab. Diagnosis. - 1998. - 1. - C.11-13).
Enzyme activity was expressed in mmol × min-1× l-1. It was obtained the value of PFA=50,14 mmol × min-1× l-1.
Because they were made conditions
a prostate biopsy was considered shown.
The patient was performed a biopsy of the prostate. The results morfologicheskogo studies of biopsy, the patient was diagnosed with adenocarcinoma of the prostate T1cN0M0.
The diagnosis is confirmed the feasibility and need for the given patient biopsy of the prostate.
In the clinic of urology of Rostov state medical University us study was conducted according to the claimed method 20 outpatients with suspected prostate cancer.
All patients received values of QC activity and the activity of the enzyme corresponded to the conditions 18,20≤QC≤58,62 and 2.16≤ACE≤51,20, so according to the claimed method biopsy of the prostate to all of them was shown.
19 patients according to morphological studies was diagnosed with prostate cancer. Thus, the accuracy of the proposed method of determination of indications for prostate biopsy was 95%. The cost of research according to the claimed methods which have amounted to two thousand rubles per patient. The proposed method does not require a staff of specially trained personnel, can be performed in an outpatient setting and, therefore, can be widely adopted in clinical practice.
The cost of conducting studies to determine the indications for prostate biopsy according to the prototype is 350-400 euros. The accuracy of the method is 89% (J. Groskopf, S.M.Aubin, J.Mathis, J.Pham, P.Hodge, M.L.Macairan, L.S.Marks, and H.Rittenhouse. 2006. APTIMA PCA3 Molecular Urine Test: Development of a Method to Aid in the Diagnosis of Prostate Cancer. Clin Chem. 52: 1089-95; Shappel S.B. Clinical utiliti of prostate carcinoma molecular diagnostic tests. Rev Urol. 2008; 10(1): 44-69). The method is difficult to implement, is not reliable enough, preventing its widespread implementation.
Thus, compared with the prototype of the proposed method for the determination of indications for prostate biopsy is more reliable, easy to implement and significantly cost-effective.
The method of determining the indications for prostate biopsy involving the study of biological material, characterized in that the secret of the prostate gland determine the activity (µmol · min-1· l-1) kallikrein (KK) and activity (µmol · min-1· l-1) angiotensin-converting enzyme (ACE) inhibitors and subject to the conditions 18,20≤QC≤58,62 and 32,16≤ACE≤51,20 biopsy of the prostate is considered shown.
SUBSTANCE: invention refers to medicine, namely to intraluminal endoscopic surgery of stomach and duodenum, and can be used in treating the patients with giant gastric and duodenal ulcers. An ulcer surface is exposed to endoscopic therapy by punctual noncontact surface monopolar coagulation with a centripetal argon plasma flame at flow rate 2.2 l/min starting in a 'SPRAY' mode at flow intensity 44 Wt, exposure length 2-3 seconds for each point. After a 30-second pause, the ulcer surface is treated with the argon plasma flame in a 'COAG' mode at flow intensity 44 Wt, exposure length 2-3 seconds for each point. 5 days later, the 'SPRAY' mode treatment of the ulcer surface is applied once more at flow intensity 40 Wt, exposure length 2-3 seconds for each point. If after 7 days following the second treatment, the monitoring fiberoptic gastroduodenoscopy shows a maximum ulcer diametre exceeding 1.5 cm, the third 'SPRAY' mode ulcer treatment is required at flow intensity 40 Wt, exposure length 2-3 seconds for each point. General coagulation time for the entire ulcer surface area in each treatment session makes 20-30 seconds.
EFFECT: method allows better treatment optimisation and improved conservative therapeutic effect in giant gastric and duodenal ulcers, as well as prevention of potential complications development.
SUBSTANCE: invention relates to field of medicine, namely to surgery. Blood is sampled from cubital vein in patients with acute pancreatitis on 1-3 and 7-10 day from disease beginning. Level of blood serum myoglobin is determined by method of performing reaction of passive hemagglutination. If level of blood serum myoglobin on 1-3 day increase from 95 to 128 ng/ml, acute fatty pancreatic necrosis is diagnosed, if myoglobin level in blood serum is higher than 128 ng/ml, hemorrhagic pancreatic necrosis is diagnosed, and if level of myoglobin in blood serum on 7-10 day increases higher than 256 ng/ml, hemorrhagic pancreatic necrosis in stage of infection of pancreatic necrosis nidus is diagnosed.
EFFECT: method allows to correct drug therapy and individually ground tactics of treating patients with acute pancreatitis depending on severity of disease form and presence of infection.
1 tbl, 3 ex
SUBSTANCE: invention relates to field of medicine, namely to diagnostic methods. Homogenate of placenta of pregnant woman who had herpes-virus infection is analysed by ELISA method for activity of granzyme B, titre of antibodies in pregnant woman's peripheral blood, and simultaneously number of syncyotrophoblast nuclei in apoptosis is calculated. If antibody titre is 1:3200 and granzyme B content is 283.14±18.00 pg/ml, percentage of nuclei in apoptosis state is 1.5±0.09%; if antibody titre is 1:6400 and granzyme B content in placenta homogenate is 712.84±23.00 pg/ml content of nuclei in apoptosis state is 2.0±0.12%; if titre of antibodies to herpes virus is 12800 and content of granzyme B in placenta homogenate is 1007.53±31.50 pg/ml (control - 251.10±31,00 pg/ml) percentage of nuclei in state of apoptosis increases to 3.5±0.08% (control - 1.0±0.009%).
EFFECT: method ensures high sensitivity of detecting nuclei in state of apoptosis in syncyotrophoblast at the background of increased content of granzyme B in placenta homogenate.
SUBSTANCE: invention relates to field of medicine, namely to laboratory analysis and can be used for prediction of course of wound process in case of purulent-inflammatory diseases if maxillofacial region of microbial etiology. In carrying out microbiological analysis of wound discharge, presence or absence of microorganisms and their concentration are determined, in case of immunologic analysis registered are total number of leukocytes, ESR, levels of medium circulating immune complexes (CIC), antibodies to streptococcus (ASL-O), immunoglobulin A (IgA). Analysis is carried out befire and on 5-7 day after operation, and in case of complete elimination of microorganisms in wound or reduction of their concentration to <103 CFU/tamp., as well as reduction in blood serum for not less than 30% of total number of leukocytes, antibodies to streptococcus, and reduction for not less than 25% of value of ESR, CIC, IgA, favourable course of wound process is predicted, in case if indices are different - prediction is unfavourable.
EFFECT: method allows to reduce time of patient's hospitalisation, carry out adequate therapy in post-operational period in due time, does not require additional equipment for carrying out microbiological and immunological analysis.
SUBSTANCE: invention relates to field of medicine, in particular to cardiology, neurology, ophthalmology, is used to carry out diagnostics of predisposition to ischemic neuroopticopathy in elderly people with ischemic heart disease (IHD), arterial hypertension (AH) and dyscirculatory encephalopathy (DEP). Blood is sampled from ulnar vein, blood samples are forms, from which DNS is separated, amplification of DNA fragments is pertformed, after that genotyping on polymorphisms del-425 and 3'-UTR of c-fms geneand polymorphism 64V of CCR2 gene is carried out, if in elderly women heterozygous genotype 64V/641 of polymorphism 64V of CCR2 gene, and heterozygous genotype 12 of polymorphism 3'-UTR of c-fms gene are detected, predisposition to ischemic neuroopticopathy is diagnosed.
EFFECT: detection of predisposition to ischemic neuroopticopathy in elderly people for administration of adequate preventive treatment.
7 tbl, 2 ex
SUBSTANCE: invention relates to medicine, namely to biochemical investigations in oncology, and can be used in determination of activation or stabilisation of pathologic process in patients with primary-resistant Hodgkin's lymphoma. In patients with primary-resistant Hodgkin's lymphoma at stages of treatment, by radioimmune method determined is content in blood of thyroid gland hormones - triiodothyronine and thyroxine, and adrenocortical hormone - cortisol, their concentration is summed up and in case of total level of hormones is within 278.2 nmole/l - 0 333.0 nmole/l conclusion about activation of malignant process is made, if total level of said hormones is 432.4 nmole/l - 833.2 nmole/l conclusion about absence of process activation is made.
EFFECT: prediction of process activation or stabilisation in patients with primary-resistant Hodgkin's lymphoma is an informative test for evaluation of pathologic process state and can be recommended for monitoring of patients at treatment stages.
1 tbl, 2 ex
SUBSTANCE: invention relates to medicine, namely to obstetrics, and can be used for prediction of preterm effusion of amniotic fluid in pregnant women with gestation term 32-36 weeks. Essence of invention lies in the following: in blood serum of pregnant women with gestation term 32-36 weeks IL-8 level is determined, and if its value equals or is lower than 15.4 ng/ml, preterm effusion of amniotic fluid is predicted.
EFFECT: method is simple in implementation and allows to predict development of preterm effusion of amniotic fluid with high accuracy, sensitivity and specificity, which makes it possible to select correct tactics of monitoring women with risk of said obstetrics pathology development, to avoid complications of labour activity and perinatal pathology.
1 tbl, 4 ex
SUBSTANCE: invention relates to field of medicine, namely to diagnostic methods and deals with method of diagnosing entry of nuclei of placenta pili syncyotrophoblast into apoptosis by determining in placenta homogenate content of IgG and IgM antibodies to phosphatidylserine in pregnant women, who had herpes-virus infection in the third trimester of pregnancy. Method lies in determination of IgG and IgM antibodies to phosphatidylserine in placenta homogenate by ELISA method. If titre of antibodies to HSV-1 equalled 1:6400, 4.70±0.08 U/ml of IgG and IgM antibodies to phosphatidylserine were detected; if titre was 1:12800, content of IgG and IgM antibodies to phosphatidylserine increased to 5.60±0.06 U/ml (control - 2.50±0.07 U/ml), number of syncyotrophoblast nuclei in state of apoptosis constituted 4.50±0.20% (control - 1.5±0.20%).
EFFECT: method possesses high sensitivity and allows to predict development of apoptosis in nuclei of placenta pili syncyotrophoblast in case if number of IgG and IgM antibodies to phosphatidylserine in placenta grows.
SUBSTANCE: invention relates to medicine, namely to biochemical investigations in oncology, and can be used in treatment of Hodgkin's lymphomas with primary drug resistance of tumour to standard schemes of drug intervention. Novelty and essence of investigation lies in the following: in patients with Hodgkin/s lymphoma at treatment stages by radioimmune method determined is content in blood of total and free thyroxine, coefficient of ratio of concentrations total thyroxine/free thyroxine is determined and if its values are within 8.4-11.2 conclusion about absence of clinical effect of anti-tumour therapy is made, if coefficient values are within 14.2-22.8, conclusion about presence of clinical effect of treatment is made.
EFFECT: laboratory biochemical analysis determines clinical efficiency of the method or its absence during treatment procedures in patients with application of standard chemichal therapy, autolymphochemical therapy and is valuable laboratory test for determination of treatment efficiency and further tactics of patients monitoring.
1 tbl, 2 ex
SUBSTANCE: invention relates to medicine, namely to neurology. Risk factors are determined from history of life: family history of cardio-vascular disease, hypertension disease and in case of its presence regularity of anti-hypertensive therapy intake, marital status, age, education, psychoemotional stress, sex, also taken into account is expression of detected during first examination cognitive impairment and results of its treatment. Their gradations and numerical values are determined, prognostic coefficients F1, F2 are calculated by mathematical formulas with further comparison of their values. If F1 is higher than F2 favourable type of cognitive impairment course in patients with ischemic stroke is predicted, and if F2 is higher than F1 - unfavourable type of cognitive impairment course.
EFFECT: method ensures possibility of estimation of clinical course of cognitive impairment, thus allowing to increase efficiency and purposefulness of preventive and medicinal measures in groups of patients with risk of developing unfavourable course of cognitive impairment.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
SUBSTANCE: method involves studying blood samples with venous blood mixed with vital stain like methylene blue. Degree of vital stain absorption by erythrocytes is determined by applying photocolorimetry. The value drop being more than 25%, extracorporal detoxication is to be predicted as ineffective.
EFFECT: simplified method.
SUBSTANCE: method involves evaluating prognostic signs and calculating their prognostic weight. Consciousness disorders and diagnosis set at prehospital stage are used as the prognostic signs. Calculations are carried out from formula: lethal outcome %= -15.99+19.1•C+14.7•DS-2.79•C•DS, where C is the ranged consciousness disorder degree, DS is the diagnosis set prehospital stage. The higher is the value, the higher is lethal outcome probability.
EFFECT: accelerated and simplified prognosis method.
SUBSTANCE: method involves evaluating proliferating processes by calculating index of positive cell nuclei (Ki-67). The Ki-67 value being from 6 to 16%, erosive ulcerating stomach lesions accompanied by stomach hemorrhage and hemorrhagic shock is to be predicted. The value being from 17 to 30%, erosive ulcerating stomach lesions without hemorrhage is to be predicted.
EFFECT: high accuracy of prognosis.
FIELD: medicine, urology.
SUBSTANCE: the present innovation deals with differential diagnostics of prostatic cancer and other prostatic diseases at the stage of primary inspection. The method includes the detection of PCA and calculation of probability coefficient for prostatic cancer (PCC) by the following formula: where e - the foundation of natural logarithm (e=2.718…), PCA - the level of total blood PCA in ng/ml, V - patient's age in years. At PCC value being above 0.2 one should diagnose prostatic cancer and to establish final diagnosis one should perform polyfocal prostatic biopsy. The method enables to increase accuracy of diagnostics at decreased number of unjustified prostatic biopsies.
EFFECT: higher efficiency of diagnostics.
SUBSTANCE: method involves determining homeostasis values and absolute number of neutrophils and lymphocytes in particular. Low neutrophil number being determined in a child during a number of years and low number of lymphocytes in the persons from the nearest child surrounding, the persons are to be examined for given psychosomatic pathology availability with following medical treatment to be applied to eliminate the detected pathology and to normalize homeostasis values and lymphocyte number among others.
EFFECT: enhanced effectiveness of prophylaxis.
SUBSTANCE: method involves measuring radio signals and low frequency spectra of signals emitted by oral cavity organs and tissues in maxillofacial area. Decoding and comparison of the spectra to healthy organs and tissues spectra is carried out. Then, modulated information signals in infra-low frequency bandwidth corresponding to healthy organs and tissues signals are applied. Low frequency radio signals and spectra are measured. Treatment with modulated signals in infra-low frequency bandwidth is applied directly to organ or its location zone via skin.
EFFECT: enhanced effectiveness in diagnosing and treating oral cavity organs and tissues in maxillofacial area.
5 cl, 3 tbl
FIELD: medical engineering.
SUBSTANCE: device has bottom and cover joined in inseparable way so that no chance is available to mismatch covers belonging to different containers. The bottom and internal part of cover are lined with sponge so that the allergenic disks are kept in uniformly wetted state. Grate with holes is attached to lower sponge, the holes narrowing downward as cones so that the allergenic disks are kept in stable vertical position.
EFFECT: simplified access to disks using forceps; excluded misidentification risk.
5 cl, 6 dwg
FIELD: medicine, operative gynecology.
SUBSTANCE: at final stage of laparoscopic operation for 5-7 min one should introduce 16 U lidase in 1 ml 2%-lidocaine solution into uterine mesentery from both sides, and then, by not removing a needle - a half of single dose of antimicrobial preparation in 1 ml 2%-lidocaine solution, then in postoperational period - an antimicrobial preparation applied during laparoscopy lymphotropically under mucosa of lateral vaginal arch from both sides for 5-7 d once daily and one antimicrobial preparation - intravenously for 5-7 d, moreover, as antimicrobial preparations one should apply gentamicin, metrogyl and other preparations permitted for intravenous application. The present innovation stimulates lymphatic drainage in area of inflammation and activates interstitial humoral transport of antimicrobial preparations that, in its turn, favors complete sanitation of inflammation foci and prophylaxis of disease relapses.
EFFECT: higher efficiency of therapy.
1 cl, 1 ex
SUBSTANCE: method involves applying biological indicator as mixture composed of 0.1% aqueous solution of amino acids: leucine, glycine, proline, serine, phenylalanine, histidine, oxyproline, arginine, glutamic amino acid and aspartic amino acid, 0.5% aqueous solution of neuromediators like dopamine and histamine, 12% aqueous solution of magnesium sulfate in proportion of amino acids : neuromediators : magnesium sulfate = 4:1:5. Indicator kept on neck surface in thyroid gland projection during 3-5min. It is dried at T=+35-40°C, studied in polarized light with quartz compensator. Columnar, columnar-and-striated, sheaf-like crystals and discharged polygonal variegated chambers being observed, thyroid gland hyperfunction is to be diagnosed.
EFFECT: high accuracy of diagnosis.