Therapeutic binding molecules in form of chimeric antibody

FIELD: medicine.

SUBSTANCE: binding molecule represents a CD45RO and CD45RB chimeric antibody. The molecule contains two domains with consistent hypervariable sites CDR1, CDR2 and CDR3, and CDR1', CDR2' and CDR3', CDR1 has amino acid sequence NYIIH, CDR2 has amino acid sequence YFNPYNHGTKYNEKFKG, and CDR3 has amino acid sequence SGPYAWFDT. CDR1' has amino acid sequence RASQNIGTSIQ, CDR2' has amino acid sequence SSSESIS, and CDR3' has amino acid sequence QQSNTWPFT. Related coding polynucleotide is described.

EFFECT: use of the invention allows to induce immunosuppression, to inhibit T-cell response and primary lymphocyte reaction in the mixed culture, to prolong survival time in mice with severe combined immunodeficiency SCID.

6 cl, 5 dwg, 2 tbl, 8 ex

 

The text descriptions are given in facsimile form.

1. Linking molecule that is a chimeric antibody to bind to the CD45RO and CD45RB, and includes
a) a first domain comprising sequentially located hypervariable sites CDR1, CDR2 and CDR3, where CDR1 has the amino acid sequence Asn-Tyr-Ile-Ile-His (NYIIH), CDR2 has the amino acid sequence Tyr-Phe-Asn-Pro-Tyr-Asn-His-Gly-Thr-Lys-Tyr-Asn-Glu-Lys-Phe-Lys-Gly (YFNPYNHGTKYNEKFKG) and CDR3 has the amino acid sequence Ser-Gly-Pro-Tyr-Ala-Trp-Phe-Asp-Thr (SGPYAWFDT); and
b) a second domain comprising sequentially located hypervariable sites CDR1', CDR2' and CDR3', where CDR1' has the amino acid sequence Arg-Ala-Ser-Gln-Asn-Ile-Gly-Thr-Ser-Ile-Gln (RASQNIGTSIQ), CDR2' has the amino acid sequence Ser-Ser-Ser-Glu-Ser-Ile-Ser (SSSESIS) and CDR3' has the amino acid sequence Gln-Gln-Ser-Asn-Thr-Trp-Pro-Phe-Thr (QQSNTWPFT).

2. The binding molecule according to claim 1, which represents a chimeric monoclonal antibody.

3. The binding molecule according to claim 1, on the expectation polypeptide, which has the sequence of SEQ ID NO:1 and/or polypeptide that has the sequence of SEQ ID NO:2.

4. The binding molecule according to claim 1, comprising a polypeptide that has the sequence of SEQ ID NO:3, and/or polypeptide that has the sequence of SEQ ID NO:4.

5. The binding molecule according to claim 3 or 4, a chimeric monoclonal antibody.

6. Polynucleotide encoding a binding molecule according to any one of claims 1 to 5.



 

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FIELD: medicine.

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22 cl, 2 dwg, 11 tbl, 6 ex

FIELD: medicine.

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36 cl, 14 dwg, 6 tbl, 13 ex

FIELD: chemistry.

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131 cl, 44 dwg, 12 tbl, 16 ex

FIELD: chemistry; biochemistry.

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7 cl, 7 dwg, 6 ex

FIELD: chemistry.

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7 cl, 7 dwg, 7 ex

FIELD: chemistry.

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33 cl, 18 dwg, 18 tbl, 24 ex

FIELD: medicine.

SUBSTANCE: method facilitates linkage of sequences, coding immunoglobulin variable regions, T-cells receptors or B-cells receptors. Method is instrument of higher effectivity for making sequence data libraries. Capability of multiple RT-PCR with chain extension by interruption with employment of matrix, derived from single cell, provides highly effective creation of sister pairs libraries.

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51 cl, 25 dwg, 27 tbl, 14 ex

FIELD: medicine.

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52 cl, 32 dwg, 7 tbl, 29 ex

FIELD: chemistry.

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9 cl, 14 dwg, 2 tbl, 13 ex

FIELD: chemistry.

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57 cl, 45 dwg, 4 tbl, 8 ex

FIELD: chemistry; biochemistry.

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60 cl, 18 dwg, 15 tbl, 8 ex

Ox40l antibody // 2395523

FIELD: chemistry; biochemistry.

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9 cl, 20 dwg, 7 tbl, 23 ex

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7 cl, 12 dwg, 1 tbl, 3 ex

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10 cl, 21 dwg, 11 ex

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8 cl, 12 dwg

FIELD: medicine.

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23 cl, 4 dwg, 5 tbl, 15 ex

FIELD: chemistry, biochemistry.

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22 cl, 13 dwg, 8 tbl

FIELD: medicine, microbiology.

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14 cl, 18 dwg, 37 ex

FIELD: chemistry.

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23 cl, 12 ex

FIELD: bioengineering.

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20 cl, 5 dwg, 2 tbl, 8 ex

FIELD: medicine.

SUBSTANCE: there are offered specific antibodies linked at least with KIR2DL1, KIR2DL2, KIR2DL3 human receptor, neutralise KIR-mediated NK cytolergy inhibition in relation to Cw3+ or Cw4+ target-cells. There are described: B-lymphocyte hybrid cell for producing the antibodies, versions of the method for producing the antibody, as well as a method for detecting a NK-cell, a method for purifying the NK-cells with the use of the antibody and versions of the pharmaceutical antibody composition. Using the antibody for preparing a medicinal agent is offered.

EFFECT: use of the invention provides producing the antibody which controls NK cytolergy of various types, intensifies cytotoxicity, increases NK cytolergy or cytotoxicity in individuals.

63 cl, 13 dwg, 3 tbl, 8 ex

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