Method of experimental estimation of resoptive function of peritoneum

FIELD: medicine.

SUBSTANCE: in order to estimate resorbtion function of peritoneum time during which animals achieve stage of surgical sleep in case of intraperitoneal introduction of ethaminal is determined.

EFFECT: method allows to estimate indirectly resoptive function of peritoneum in norm and under impact of aggressive factors.

 

The invention relates to experimental medicine and can be used for experimental evaluation of osteoclast function of the peritoneum.

Currently, the only approach to the evaluation of the function of the peritoneum is the clinical definition of peritoneal transport when conducting in patients with chronic peritoneal dialysis. The method consists in the following. The peritoneal cavity within 20 minutes emptied after a night cycle dialysis, weighed 2 l capacity warmed dialysis solution with 2.5% glucose, the contents of the tank is introduced into the peritoneal cavity for a 10-minute period, during which the patient takes a sample of venous blood at 4 hours after injection of dialysate last write for 20 minutes to obtain a sample of dialysate, blood samples and dialysate analyzed for creatinine and glucose, and derived the capacity of dialysate weighed to determine the scope of ultrafiltrate. On the basis of the received data is evaluated level of peritoneal transport: very high, high, medium, low, very low [Gurevich KA et al. Peritoneal dialysis: guidelines for doctors. St. Petersburg, 2003, p.14-15]. However, this method does not sell in the experiment on animals, requires the installation of intra-abdominal catheter, prolonging Polop the operating inflammation, and consequently, affect the validity of the research.

The aim of the proposed method is to assess osteoclast function of the peritoneum in the experiment.

The objective is achieved by estimating the time period from the moment of intraperitoneal administration of a solution of etamine before the stage surgical sleep Atamanova anesthesia.

The essence of the proposed method is that the method of experimental evaluation of osteoclast function of the peritoneum, including the definition of time the animal stage surgical sleep with intraperitoneal injection of etamine indirectly allows to evaluate osteoclast function of the peritoneum in normal conditions and when exposed to aggressive factors (such as the surgical trauma).

The method is as follows.

After preparation of the surgical field in the area of the front surface of the abdomen, including shaving the wool of the animal, the treatment of the surgical field antiseptics, you lparameter the anterior abdominal wall by lifting it in the form of folds. The puncture needle of the abdominal wall is on the boundary between the upper 2/3 and lower 1/3 of the white line of the abdomen, at the edge of the formed skin folds that provide the minimum risk of injury to the internal organs of the abdominal cavity. After the needle is injected into the abdominal on the spine of a standard solution of etamine at a dose of 40 mg/kg From this moment begins the countdown to the onset of stage surgical sleep Atamanova anesthesia. Criteria for surgery under General anesthesia were: the lack of active movements, reactions to external stimuli, and corneal blink reflex, respiratory arrhythmia. Certain data by the time interval corresponds to the speed of resorption of etamine through the membrane of the peritoneum from the abdominal cavity into the bloodstream. Repeated studies allow us to determine the dynamics of the peritoneal resorption both in normal conditions and when exposed to various aggressive factors (such as the surgical trauma).

Method experimental evaluation of osteoclast function of the peritoneum, characterized in that the evaluation time period from the moment of intraperitoneal administration of a solution of etamine before the stage surgical sleep Atamanova anesthesia.



 

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