Method of restoring reducing with age cell growth of tissues (tissue rejuvenation)
SUBSTANCE: invention relates to experimental medicine and deals with restoration of reducing with age potential of cell growth of tissues in old laboratory animals. For this purpose medication "Transfer-factor" is introduced to laboratory animals in dose corresponding to 1 capsule a day per 50 kg of weight.
EFFECT: method ensures activation of T-lymphocyte function and restoration of potential of cell growth of salivary gland tissues in said group of animals.
The invention relates to the fields of biology and medicine.
Analogs of the invention has: all currently used tools anti-aging" can't pay the most important, Central to the process of aging is a decline with age pack of tissues by the division and growth of cells (a sharp decline with the age of the potential of cell growth).
Offered to restore declining with age, the capacity of the cellular tissue growth (rejuvenation of tissues) to use immunotropic drug "transfer Factor"derived from cow colostrum and restoring a number of indicators of immunity, reduced as a result of various reasons; a drug used in foreign practice for medicinal purposes in various diseases with immune deficiency are not directly associated with the aging process.
The way to restore declining with age, the capacity of the cellular tissue growth (rejuvenation of tissues) is that for the treatment of age-related changes in tissues used the drug "transfer factor" in a dose of 1-3 capsules per weight of 60-80 kg (doses correspond to the standard recommendations for use of the drug in chronic diseases of different nature).
Example 1. EFFECTS OF TRANSFER FACTOR IN AGED MICE.
Mice Balb/c, females (6 test old and 6 experienced, as well as 12 young control), in TRUSTe 12 months, introduced TF dissolved in physiological solution, 1 time a day for 1 week. at a dose similar to that used in humans (1 capsule per 50 kg of body weight).
As a test potential of cell growth used hyperplastic reaction of the tissue of the salivary glands on isoproterenol, known as "the phenomenon of Selye (increase in weight after 24 hours).
Investigated also the weight of thymus and spleen, the amount of mononuclear cells from the spleen in the density gradient ficoll 1,09 (lymphocytes + And-cells) and density 1,065 (activated cells of the spleen with lower density)and the number of A-cells in the spleen (adhering to the surface of a plastic Petri dish).
It was shown that if young mice introduction of isoproterenol causes an increase in the mass of the salivary glands 1.5-1.65 times, in old animals is observed in all cases, the mass reduction of the salivary glands on the introduction of isoproterenol (6-26%) from the mean in the control group of animals).
Introduction TF restored the capacity of cell growth in aged mice all animals on isoproterenol an increase in the mass of the salivary glands in a 1.46-1.56 times.
At the same time dramatically (several times) increased the number of activated spleen cells, as well as decreased reaction thymus (weight) on the stress on the introduction of adrenomimetic - Sopater is Nola. Observed number of positive changes And cells.
Thus, TF stabilized and activated the function of T-lymphocytes old animals and restored cell growth potential of tissue that is the most promising of the known effects in Anti-Ageing Medicine" (new industry - "Medicine anti-aging"), and also creates the possibility of activating cell growth in various pathological processes in order to healing tissues - bone (fractures), wound healing (including operating), lung tissue (pneumonia) and other
The way to restore declining with age, the capacity of cell growth in tissues of the salivary glands in the experiment, characterized in that laboratory animals injected the drug "transfer factor" in a dose corresponding to 1 capsule per day per 50 kg of body weight.
SUBSTANCE: invention relates to chemical and pharmaceutical industry, namely to creation of medication reducing hematotoxicity of cytostatics. As such medication, polysaccharides from leaves of coltsfoot (Tussilago farfara L.) are used.
EFFECT: medication extends arsenal of preparations increasing efficiency of chemical therapy by reducing henatotoxicity of cytostatics.
SUBSTANCE: invention relates to field of medicine, namely to gynecology, and deals with treatment of complicated forms of inflammatory diseases of uterine appendages. For this purpose, when performing abdominal operation from two sides between round ligament of the uterus and isthmic part of fallopian tube medicinal mixtures of the following medications are introduced: at first - lidase 16 U in 1 ml of 0.25% Novocain, after that without removing the needle prednisolone 15 mg, amikacin 0.25 mg in 1 ml of 0.25% Novocain. Drainage of small pelvis with gauze-glove pad on lateral pockets to retrouterine space and bed of remote mass is carried out. In post-operational period antimicrobial drug is introduced intravenously. Limphotropically from two sides from anterior commisure of large pudental lips introduced are medicinal mixtures of medications: first - lidase 16 U in 1 ml of 0.25% Novocain, after that without removing the needle - prednisolone 15 mg, amikacin 0.25 mg in 1 ml of 0.25% Novocain, slowly during 15 minutes. Such introduction is carried out 1 time a day for 7-8 days.
EFFECT: method ensures stimulation of interstitial humoral transport and lymphatic drainage and fast normalisation of clinical and laboratory parametres with reduction of course dose of antimicrobial drugs.
SUBSTANCE: invention relates to veterinary. Method of manufacturing biogenic stimulator, which includes 50% of biologically active mass from drone larvae, 49.7% of sodium chloride solution and 0.3% of preservative, consists in the following: in order to obtain preparation comb with alive healthy 18-22 days old drone brood is kept in refrigerator at temperature 3-4°C during 5-6 days, after that comb with brood is opened and drone larvae of the same size, light-gray colour are selected, after which drone larvae are crushed in sterile laboratory mill, diluted with sterile sodium chloride solution with concentration 0.9% in ratio 1:1 and autoclaved at temperature inside boiler 120°C and steam pressure in jacket 1.5 atmosphere for one hour, after that the mass is filtered through two layers of gause into sterile measure vessel, brought to initial volume with sterile solution of sodium chloride with concentration 0.9%, preservative (phenol) is added, preparation is poured into prepared sterile vessels observing rules of asepsis, vessels are hermetically closed, autoclaved at 120°C and steam pressure in jacket 1.5 atmosphere for 20 minutes.
EFFECT: method allows to reduce considerably terms of preparation manufacturing, increase its safety, increase storage terms Biogenic stimulator obtained in said way contributes to improvement of animal growth and development, increase of offspring preservation, reduction of feed cost per unit of body weight increase, increase of general and specific resistance of organism.
3 tbl, 6 ex
SUBSTANCE: group of inventions relates to medicine, namely to nutrition and therapy, and can be used for reduction of unfavourable effects in case of treatment with phytosterols. In accordance with invention method deals with maintenance and/or increase of bioacceptibility and/or levels in blood of at least one of circulating lipophilic vitamins, lipophilic medications, lipophilic nutrients or any lipophilic pharmaceutically active ingredient in individual who needs therapy with phytosterols by introduction of therapeutically efficient amount of mixture of phytosterol ester(s) (PS-E) and 1,3-diglyceride(s) (DAG). Edible composition in accordance with invention contains mixture of phytosterol ester(s) (PS-E) and 1,3-diglyceride(s) (DAG).
EFFECT: application of inventions allows to reduce concentration of cholesterol and triglycerides without unfavourable impact on the level of lipophilic molecules in blood.
28 cl, 5 tbl, 2 dwg, 5 ex
SUBSTANCE: invention pertains to a polymorphous modification of A N-benzyl- N'-(2,6-diisopropyl-phenyl)-N-isopropyl-malonamide, which is characterised by X-ray powder diffraction pattern with characteristic peaks at approximately 16.0, 19.6 and 28.1 for 2θ degrees.
EFFECT: obtaining a composition which can be used to make a medicinal agent for reducing secretion of sebum and treating skin disorders.
6 cl, 4 ex, 3 dwg
SUBSTANCE: invention refers to medicine, dentistry and can be used for treatment of inflammatory periodontal diseases. Luminol-dependent chemiluminescence of oral fluid is carried out. Rate of maximum flash and glow light sum is estimated. With the value of maximum flash from 0.8 to 5 c.u. (conventional units) and light sum of 1.5 to 37 c.u. an impact of magneto-infrared laser radiation is conducted. The impact is conducted with a pulse repetition rate of 80 Hz, LED radiating power of 90 mW. The impact is conducted in area of projection of the foci and in field of angle of the mandible on the same side with exposure 2 minutes per point, and then in the spinous process of third cervical vertebra during 1 minute and in oral tissues for 2 minutes. After that it is affected with magneto-infrared laser radiation with frequency of 150 Hz, LED radiating power of 50 mW. The impact is conducted on reflex points GI4 and E36 symmetrically, VC12 or MC6 and RP6 symmetrically, VC12, alternating every other day. At the same time in each session an effect is exerted on points CI 1, GI 20, E 5, V 10. The treatment course is 10 procedures. Exposure is 0.5 minutes on one point. At the maximum flash rate from 6.55 to 25 c.u. and light sum from 26 to 45 c.u. a biologically active food supplement "PropoFlan" is administered in a dose of 2 tablets 3 times a day for 1 month.
EFFECT: method provides choice of adequate treatment of inflammatory periodontal diseases taking into account the activity of oxidative processes in oral fluid.
SUBSTANCE: invention refers to medicine, namely to out-patient anaesthesiology, also can be used as an anaesthesia care in out-patient dental interventions. That is ensured by premedication with administered hypoxen in dosage 1g, 60 minutes prior to the intervention.
EFFECT: method ensures correction of stress-specific cardiac rhythm disturbance in the given category of patients due to antiarrhythmic actions of hypoxen, caused by decreased spontaneous activity of the cardiac pacemaker in a healthy and ischemic heart.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, namely to cardiology and can be used for connective tissue density correction following myocardial infarction and pericardiotomy. The method involves triple administration of an inhibitor of a polypeptide sequence containing fragments -Asp-Pro-Lys-Arg-Leu-Tyr-Cys-Asn-Gly-Gly-, -Glu-Arg-Gly-Val-Val-Ser-Ile-Lys-Gly- and -Ala-Asn-Arg-Tyr-Leu-Ala-Met-Lys-Glu-Asp-Gly-Arg-Leu- and having 100 to 240 amino-acid residues in 1.5 and 6 hours and in 3 days after infarction and pericardiotomy started.
EFFECT: use of the invention allows restructuring the forming connective tissue due to changing of the polypeptide sequence concentration in certain distances of a pathological process and thereby reducing fibrosis following myocardial infarction and pericardiotomy.
4 cl, 2 tbl, 5 dwg, 2 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medicine, particularly pharmacology, namely to agents for treating the remote consequences of methemoglobin former poisoning. Chinese magnolia tincture is offered as said agent.
EFFECT: preparation of the antihypoxic, antioxidant, hepatoprotective and nephroprotective agent for treating the remote consequences of methemoglobin former poisoning.
5 ex, 5 tbl
FIELD: medicine, pharmaceutics.
SUBSTANCE: there is offered an agent stimulating hemopoietic stem cells (HSCs) emission in blood flow; said method is characterised by the fact that it contains at least one phosphorylated polyisoprenoid presented with polyprenylphosphate or dolichil-phosphate with N isoprene links in number 7 to 22.
EFFECT: invention provides 6-14-fold increase of HSCs number in blood flow, and the agent works permanently without considerable changes in bone marrow and spleen for restoring action in bone marrow transplantation, in treatment of severe infectious diseases.
2 cl, 4 tbl, 4 ex
SUBSTANCE: invention is a skin moistening agent for oral delivery comprising fermented milk whey as active component and obtained by milk fermentation using the strain Lactobacillus, helveticus, CM-4 (deposited with International depositary institution for patents by National Institute of advanced science and technology under inventory number FERM BP-6060).
EFFECT: invention provides skin moistening.
6 cl, 2 ex, 5 tbl
SUBSTANCE: invention relates to medicine, namely to gynecology, and concerns remedy for treating bacterial vaginosis, which contains fermented mare's milk of 2-3 day ageing with proteinase activity 475.5 units, obtained using microorganism Lactobacterium bulgaricum and yeasts of genus Torula, and includes lactic acid, ascorbic acid, panthotenic acid, riboflavin, thiamine, vitamin B 12. Course of treatment is 10 days.
EFFECT: invention ensures fast vagina sanation and recovery of its normal microflora.
2 cl, 1 ex, 2 dwg
SUBSTANCE: invention relates to medicine, namely to pediatrics, and concerns prevention of acute respiratory viral infections in pre-school children. For this purpose on the background of vitamin therapy child is prescribed 150 ml of cultured milk product of clinical preventive nutrition bifilact "Biota", mixed with 30 ml of fermenting regenerated live biomass Enterogs with 3-4 weeks course.
EFFECT: said treatment scheme ensures increase of child organism resistance due to efficient correction of intestine disbios by increased concentration of bifidum- and lacto- bacteria with minimal load on fermentative system of gastrointestinal tract.
2 tbl, 2 ex
SUBSTANCE: mare's milk concentrate which has been dried up at temperature from 10 to 50°C at pressure from 1 to 50 mb, on biologically inert, highly dispersive matrix being a highly dispersive dioxide of silicon, apply for obtaining of a preparation for oral ingestion at treatment of diseases of neurodermite and psoriasis. The preparation additionally contains irreplaceable amino acids, hydrocarbonate, potassium, carbonate, citrate, calcium, magnesium, vitamin C, vitamin E, niacin, zinc, iron, beta carotin, pantothenic acid, manganese, vitamin B6, B2, B1, B12, copper, sodium, a biotin, Acidum folicum, molybdenum, selenium, xanthan, fructose, lemon acid, or from both such substances.
EFFECT: application of mare's milk concentrate is oral for neurodermite and psoriasis treatment, firmness at storage and maintenance of high-quality components in the concentrated form.
6 cl, 7 tbl, 6 ex
FIELD: medicine; hygiene.
SUBSTANCE: application of an extract of the milk obtained from milk, at least, of one animal family horse for manufacturing of a preparation for sexual hygiene, containing 0.1-20 wt % of the specified extract from mass of a solid and a preparation for the sexual hygiene, containing such extract of milk is offered. The preparation can represent female disinfectant gel, female disinfectant shampoo, female disinfectant soap, vaginal disinfectant liquid, vaginal suppository, vaginal pill, concentrate, sparkling tablet, powder, cool-dehumidified lactic acid bacillus preparation etc. The preparation can be executed in the form of an adsorbing material, dry or wet napkin, hygienic lining or a vaginal tampon.
EFFECT: preparation is effective for prevention; treatments and aftertreatment of vaginitis and vulvitis; promotes conservation eubacteriosis of an organism and effectively prevents vaginal dryness.
16 cl, 6 ex, 1 tbl
FIELD: medicine, in particular balneology and physical therapy.
SUBSTANCE: claimed additive contains dihydroquercetin or mixture of dihydroquercetin and milky whey in ratio from 2:1 to 1:2 as biologically active additive for balneological bath agent. Dalneological bath agent includes base, osmolytically active components such as glycerol and inorganic salts, as well as biologically active additive of plant and/or animal origin, wherein osmolytic activity is 7700-11000 mOsm/l. As biologically active additive of plant and/or animal origin it contains dihydroquercetin or mixture of dihydroquercetin and milky whey in ratio from 2:1 to 1:2 and as the base it contains water and/or concentrated milk in the next component ratio (mass %): glycerol 25.0-30.0; inorganic salts 10.0-15.0; dihydroquercetin or mixture of dihydroquercetin and milky whey 0.2-1.0; and balance: water and/or concentrated milk up to 100 %.
EFFECT: new effective additive for balneological bath agent.
5 cl, 9 ex
FIELD: pharmaceutical industry, medicine.
SUBSTANCE: claimed agent contains 2-3-day kumiss with specific proteinase activity, obtained by using Latobacterium bulgaricum microorganisms and genus Torula yeast and containing lactic acid, ascorbic acid, panthothenic acid, riboflavin, thiamine, vitamin B12 in specific component ratio.
EFFECT: effective agent for topical treatment of pyoinflammation diseases of soft tissues; decreased treatment time.
FIELD: medicine, veterinary.
SUBSTANCE: milky whey apoproteins are useful to inhibit pathogen adhesion to/on human or animal body surface. Milky whey apoproteins are used to produce pharmaceutical preparation to inhibit pathogen adhesion to/on human or animal body surface. Pharmaceutically acceptable system for delivery of apoprotein includes one milky protein or mixtures thereof and pharmaceutically acceptable carrier. Agent for infection prophylaxis or treatment contains one milky apoprotein or mixture thereof and fatty acid or mixture thereof, or monoglyceride or mixture thereof.
EFFECT: effective agent for prophylaxis or treatment of human or animal infection.
35 cl, 17 dwg, 5 ex
FIELD: cosmetic industry.
SUBSTANCE: it is necessary to carry out immobilization of native casein of goat milk at 50°C obtained as a result of flocculation onto methyl cellulose followed by introducing fucoidane hydrolyzate, silver nitrate and glycoproteins of mucus of animal origin at the ratio of components that provide the following ratios of cream's components, weight%: glycoproteins of mucus of animal origin 13.0-15.0; native casein of goat milk 30.0-35.0; methyl cellulose 30.0-35.0; silver nitrate 2.0-2.5; fucoidane hydrolyzate 15.0-22.5.
EFFECT: higher efficiency.
7 cl, 2 ex, 1 tbl
SUBSTANCE: method involves carrying out immobilization of protein on methylcellulose used as a high-molecular compound and modification of its surface. Goat native milk casein prepared by flocculation is immobilized on its surface at temperature 50°C. Then silver nitrate and fucoidan are added in the ratio of components providing the following ratio of components in the agent, wt.-%: goat native casein, 40-45; methylcellulose, 40-45; silver nitrate, 0.5-2.5, and fucoidan, 9.5-17.5. Invention provides expanding antibacterial and wound-healing agents.
EFFECT: improved preparing method of agent.
7 cl, 1 tbl, 3 ex
FIELD: veterinary science.
SUBSTANCE: one should collect summer colostrums in cows to be filtered, poured into sterile tanks per 1-1.5 l and frozen in freezing chambers at -20 - -22 C. In winter-spring period colostrums should be gradually defrosted while calves are being calved. To remove casein in raw material one should apply abomasal enzyme - pepsin (4-6 g pepsin/l colostrum). This enzyme should be added into colostrums heated up to 38 C. After casein precipitation on should filter the whey to be conserved with potassium sorbate (1 g potassium sorbate/l whey). The method is very simple and enables to obtain high-quality preparation.
EFFECT: higher efficiency.
1 dwg, 1 ex, 1 tbl