Ox40l antibody

FIELD: chemistry; biochemistry.

SUBSTANCE: invention relates to biotechnology and is an antibody which bonds with OX40L and variants of this antibody which contain certain Fc-fragments obtained from the human body and do not bond with the complement factor Clq. The monoclonal antibody is produced by a cell line selected from a group which includes cell lines deposited in the German collection of microorganisms and cell cultures (DSMZ) under inventory numbers No. DSM ACC 2685, DSM ACC 2686, DSM ACC 2688, DSM ACC 2689. The invention also relates to a method of obtaining such antibody, to nucleic acid molecules which code the disclosed antibody. The disclosed antibody has an advantage for patients suffering from inflammatory diseases.

EFFECT: antibody is used in diagnostic composition for detecting OX40L in vitro, in a pharmaceutical composition for preventing and treating inflammatory diseases, as well as in preparing a medicinal agent for preventing and treating inflammatory diseases.

9 cl, 20 dwg, 7 tbl, 23 ex

 

The text descriptions are given in facsimile form.

1. The antibody that binds to human OX40L, characterized in that it contains a combination of variable regions, independently from each other selected from the group comprising the following combinations:
a) a variable region light chain that has the amino acid sequence SEQ ID NO1, and the variable region of the heavy chain, which has the sequence of SEQ ID NO:2;
b) variable region light chain that has the amino acid sequence of SEQ ID NO:3, and the variable region of the heavy chain, which has the sequence of SEQ ID NO:4;
C) variable region light chain that has the amino acid sequence of SEQ ID NO:5, and the variable region of the heavy chain, which has the sequence of SEQ ID NO:6;
d) variable region light chain that has the amino acid sequence of SEQ ID NO:7, and the variable region of the heavy chain, which has the sequence of SEQ ID NO:8;
d) variable region light chain that has the amino acid sequence of SEQ ID NO:9, and the variable region of the heavy chain, which has the sequence of SEQ ID NO:10;
e) variable region light chain that has the amino acid sequence of SEQ ID NO:11 or 16, and the variable region of the heavy chain, which has the sequence of SEQ ID NO:12.

2. The antibody that binds to human OX40L, characterized in that it is produced by a cell line selected from the group comprising cell line
hu-Mab < hOX40L>LC.001,
hu-Mab < hOX40L>LC.005,
hu-Mab < hOX40L>LC010,
hu-Mab < hOX40L>LC.019,
hu-Mab < hOX40L>LC.029
and hu-Mab < hOX40L>LC.033.

3. The antibody that binds to OX40L, characterized in that it belongs to the class of people who ekeskog IgG1 and contains as
a) γ heavy chain SEQ ID NO:58 and Kappa light-chain SEQ ID NO:61,
b) γ heavy chain SEQ ID NO:62 and Kappa light-chain SEQ ID NO:65 or
in) γ heavy chain SEQ ID NO:66 and Kappa light-chain SEQ ID NO:69.

4. The nucleic acid molecule encoding the antibody molecule, characterized in that the antibody binds to human OX40L and contains a combination of variable regions, independently from each other selected from the group comprising the following combinations:
a) a variable region light chain that has the amino acid sequence of SEQ ID NO:1, and the variable region of the heavy chain, which has the sequence of SEQ ID NO:2;
b) variable region light chain that has the amino acid sequence of SEQ ID NO:3, and the variable region of the heavy chain, which has the sequence of SEQ ID NO:4;
C) variable region light chain that has the amino acid sequence of SEQ ID NO:5, and the variable region of the heavy chain, which has the sequence of SEQ ID NO:6;
d) variable region light chain that has the amino acid sequence of SEQ ID NO:7, and the variable region of the heavy chain, which has the sequence of SEQ ID NO:8;
d) variable region light chain that has the amino acid sequence of SEQ ID NO:9, and the variable region of the heavy chain, which has the sequence of SEQ ID NO:10;
e) vari is belna region light chain, which has the amino acid sequence of SEQ ID NO:11 or 16, and the variable region of the heavy chain, which has the sequence of SEQ ID NO:12.

5. The nucleic acid molecule encoding an antibody that binds to human OX40L, characterized in that it encodes the antibody, including:
a) γ heavy chain SEQ ID NO:58 and Kappa light-chain SEQ ID NO:61,
b) γ heavy chain SEQ ID NO:62 and Kappa light-chain SEQ ID NO:65 or
in) γ heavy chain SEQ ID NO:66 and Kappa light-chain SEQ ID NO:69.

6. A method of producing an antibody that binds to OX40L and contains the Fc fragment derived from the human body, wherein the cultured cell host comprising a vector containing a nucleic acid molecule according to claim 4 or 5 under conditions that provide for synthesis of antibodies and secrete antibody from the culture.

7. Diagnostic composition for the detection of OX40L in vitro containing the antibody molecule according to any one of claims 1 to 3 in dose 0.1 mg/kg and at least one diagnostically acceptable excipient.

8. The pharmaceutical composition intended for the prevention or treatment of inflammatory diseases containing the antibody molecule according to any one of claims 1 to 3 in dose 0.1 mg/kg and at least one diagnostically acceptable excipient.

9. The use of antibodies according to any one of claims 1 to 3 for the preparation of drugs is, intended for the prevention and treatment of inflammatory diseases.



 

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