Method of simulation of chemical gastritis in experiment
SUBSTANCE: for simulation of chemical gastritis in nonlinear white rats a mixture of 200 mcl of 5% solution of ascorbic acid and nonsteroidal antiinflammatory preparation Ketorol are administered orally during 21 days at the rate of 5 mg/kg 1 hour prior to feeding once a day.
EFFECT: adequate reproduction of the model.
The invention relates to medicine, namely to gastroenterology, pathological physiology and experimental medicine and can be used to study the pathogenesis of chemical gastritis and preclinical efficacy studies of new methods of treatment of this pathology.
Today there are several options for modeling chemical gastritis in the experiment. The closest analogue is the way of modeling the chemical gastritis in rats male Wistar by simultaneous introduction of animals nonsteroidal anti-inflammatory drugs and medical bile (Knyshov CENTURIES the Influence of boron mineral water on the status of processes of lipid peroxidation and antioxidant factors of protection in experimental gastroduodenitis // Questions of balneology, physiotherapy and exercise therapy. - 2002. No. 2. - P.34-36).
The disadvantage of this method is the absence of such pathognomonic for chemical gastritis and histological signs as hyperplasia pit epithelium with local depletion of mucin, hypertrophy of the smooth muscle with the least pronounced swelling and inflammation in its plate mucosa, increase in its plate gastric mucosa in the number of smooth muscle cells (Sobala, G.M. Reflux gastritis in the intact stomach / Sobala G.M., King, R.F., Axon, A.T., Dixon M.F. // J. Clin. Pahol. - 1990, Apr. - Vol.43. - 34. - P.303-306 Compton K.K. Gastritis: new pathological classification and diagnosis / K. Compton // ROS. Journe. Gastroenterol., gepatol., coloproctol. - 1998. No. 3. - P.84-80). On the contrary, the author notes the emergence of a diffuse lymphocytic-histiocytomas and plasmocytomas inflammatory infiltration own plates mucous stasis and small hemorrhages, cubic transformation of epithelial and glandular epithelium, which is not typical for chemical gastritis. Therefore, in modern conditions of development of optimal, approximate to the real conditions of option modeling of chemical gastritis is an important task.
Effect: create a model, which reflects the morphological picture of the destruction of the gastric mucosa in chemical gastritis in humans.
This task is achieved by oral administration of nonlinear white rats for 21 days of a mixture of 200 μl of 5% solution of ascorbic acid and non-steroidal anti-inflammatory drug Ketorol®" ("Dr. Reddy with laboratories Ltd., India, haiderabad, active substance - Ketorolac tromethamine) at a rate of 5 mg/kg for 1 h before feeding 1 time per day.
The method is as follows. Nonlinear white rats (males and females) within 21 days orally administered mixture of 200 μl of 5% of the races is the thief of ascorbic acid and non-steroidal anti-inflammatory drug Ketorol rate of 5 mg/kg for 1 h before feeding 1 time per day. At the end of the experiment animals slaughtered under ether anesthesia with observance of the rules of euthanasia, the material (stomach wall) fixed in 10% buffered by Lilly (pH 7,2) formalin. Using conventional techniques, prepare histological specimens, which are revealed typical histological signs of chemical gastritis: the pit epithelium hyperplasia with local depletion of mucin, the increase in the number of tuck-in (parietal) cells in the bottom of the glands of the mucous membrane of the stomach pyloric hypertrophy of muscle plate of the gastric mucosa, increase in its plate gastric mucosa in the number of smooth muscle cells, edema own plates mucous membrane.
The experiment was performed on 30 animals - male and female outbred white rats four months of age contained in standard vivarium conditions. All experiments were conducted in accordance with "the Rules of work with the use of experimental animals" (Annex to order of the Ministry of health of the USSR from 12.08.1977, N 755) and the European Convention for the protection of vertebrate animals used for experimental and other scientific purposes" dated March 18, 1986
Animals were divided into 2 experimental groups, the composition of the groups was as follows:
Group I - control (INTA the local animals), n=15;
Group II - animals treated for 21 days orally 1 time a day for 1 hour before feeding a mixture of 200 μl of 5% solution of ascorbic acid and non-steroidal anti-inflammatory drug Ketorol®" ("Dr. Reddy with laboratories Ltd., India) at a rate of 5 mg/kg, n=15.
On the 22nd day from the beginning of the experiment, animals were taken from experiment by transection of the spinal cord under ether anesthesia with observance of the rules of euthanasia. When conducting autopsy histological samples obtained from different parts of the rat stomach (esophageal, cardiac, pyloric departments)were fixed in 10% buffered by Lilly formalin (pH of 7.2), obezvozhivani in ascending alcohols fortress, were placed in paraffin, and sections (3-4 μm) were obtained on a rotary microtome, were applied to the glass with adhesive coating (egg albumin), were stained with hematoxylin and eosin and pikrofusin by van gieson.
When conducting a histological study in all animals treated within 21 days of the mixture of 5% ascorbic acid and non-steroidal anti-inflammatory drug Ketorol, noted the appearance of histological signs pathognomonic for chemical gastritis: the pit epithelium hyperplasia with local depletion of mucin, reducing the number of main cells in the bottom of the glands of the mucosa of the pyloric stomach, hypertro the Oia muscle plate of the gastric mucosa, the increase in own plate of gastric mucosa in the number of smooth muscle cells and edema.
Examples of specific performance
Example 1. Male white rats weighing 210 g for 21 days orally 1 hour before feeding 1 time per day was administered a mixture of 5% solution of ascorbic acid and non-steroidal anti-inflammatory drug Ketorol rate of 5 mg/kg On the 22nd day the animal was killed under ether anesthesia by transection of the spinal cord, performed histological examination of the stomach wall.
In the study of histological specimens in transmitted light of the light microscope revealed visible plethora of vessels and edema own plates mucosal and submucosal basis gastric esophageal, cardiac, fundic and pyloric regions. In the glandular mucosa of the stomach was observed pit epithelium hyperplasia, a local decrease in the number of mucous cells, the increase in the number of parietal cells in the bottom of the glands of the gastric mucosa, muscle hypertrophy plate mucosa, the emergence of the private record of the mucous membrane of the stomach of a large number of smooth myocytes. Identified changes are consistent with the histological picture of NSAID gastropathy.
Example 2. The female white rats weighing 178 g for 21 days PE is given orally 1 hour before feeding 1 time per day was administered a mixture of 5% solution of ascorbic acid and non-steroidal anti-inflammatory drug Ketorol®at the rate of 5 mg/kg On the 22nd day the animal was killed under ether anesthesia by transection of the spinal cord in compliance with the rules of euthanasia performed histological examination of the stomach wall.
Microscopic examination of histological samples noted marked plethora of vessels and edema own plates mucosal and submucosal basis gastric esophageal, cardiac, fundic and pyloric regions. In the glandular portion of the gastric mucosa revealed hyperplasia pit epithelium, a local decrease in the number of mucous cells, reducing the number of main cells due to the predominance of tuck-cells in the bottom of the glands of the mucous membrane of the stomach pyloric hypertrophy in the muscle plate of the mucous membrane, the emergence of the private record of the mucous membrane of the stomach of a large number of smooth muscle cells.
Thus, this animal daily oral administration of 5% solution of ascorbic acid in combination with nonsteroidal anti-inflammatory drug ("Ketorol®") has led to the development of chemical gastritis, which is confirmed by histological examination.
The positive effect from the use of the proposed method is to create experimental models of chemical gastritis, with 100%reproducibility and corresponding histological picture of the chemical gastritis, observed in humans.
A method for simulating chemical gastritis in the experiment by oral administration to rats nonsteroidal anti-inflammatory drug, wherein the nonlinear white rats for 21 days to enter a mixture of 200 μl of a 5%solution of ascorbic acid and non-steroidal anti-inflammatory drug Ketorol rate of 5 mg/kg for 1 h before feeding, 1 time per day.
SUBSTANCE: registration of fluorescence in vivo and noninvasive is conducted with method of diffusion fluorescence tomography using device where radiation source and radiation detector are located in configuration "peek-a-boo". Analysis of fluorescence intensity is conducted with images of an animal in the frontal plane. Fluorescence registration is conducted both in abdominal cavity organs and in tense areas of skin and abdominal region as a whole. Armpits skin is used as tense areas of skin.
EFFECT: method enables to carry out studies in vivo and non-invasively, to assess accumulation of photosensitiser in skin to select a photosensitiser with minimal skin phototoxicity, enables to assess accumulation of photosensitiser in tissues without using identification of organs while studying dynamics of accumulation and excretion of photosensitisers.
5 ex, 8 dwg
SUBSTANCE: invention relates to experimental medicine, and can be used for protection of post-vaccination immunity to tetanus anatoxin against methotrexate-induced depression. Method includes parenteral introduction of miliacin into experimental animal organism in dose 2 mg/kg of weight. Miliacin is introduced tree times daily to animals with formed post-vaccination immunity to tetanus anatoxin, subjected to immunodepressive impact of methotrexate cytostatic.
EFFECT: application of invention allows to protect formed post-vaccination immunity against immunodepressive impact of cytostatic due to anticatabolic action of miliacin.
SUBSTANCE: invention relates to medicine, namely to experimental surgery, and can be used for correction of ischemic liver affection. For this purpose 2 hours before operation 5% mexicor solution in dose 10 mg/kg of weight is intravenously jet injected to experimental animal. After that one hour before operation actovegin in dose 10 mg/kg of weight is intravenously jet injected. During operation ischemia is created by temporal extracorporal portacaval shunting. In post-operation period each of preparations is introduced 1 time a day during 7 days. Interval between their introduction is 12 hours: mexicor is introduced at 8 o'clock, actovegin - at 20 o'clock.
EFFECT: method ensures reduction of reperfusion disorders, elongation of safe terms of liver exsanguination up to 30 minutes and prevention of massive hemorrhage in operations on this organ.
3 dwg, 4 tbl, 3 ex
SUBSTANCE: invention relates to medicine, namely to treatment of infectious diseases, and can be used in determination of non-specific anti-infectious action of immunomodelling immunobiological preparation (IIP). Essence of method consists in the following: IIP in therapeutically efficient for immunocorrection day dose is perorally introduced to a group of 5-7 monkeys with diarrhea syndrome, in combination with efficient in case of diarrhea syndrome antibiotic, in day dose, which constitutes 1/2 of the dose efficient in case of diarrhea syndrome. After that in case if carried out treatment is inefficient during 2 days, day dose of antibiotic is increased to 3/4 of the dose efficient in case of diarrhea syndrome. Determined is either absence of non-specific anti-infectious IIP action in case when combined application of IIP and antibiotic proves to be inefficient during 7 days, or presence of non-specific anti-infectious IIP action in case when combined application of IIP and antibiotic proves to be efficient.
EFFECT: method allows to determine non-specific anti-infectious IIP action due to determination of minimal efficient in case of diarrhea syndrome day dose of antibiotic in its combination with IIP
SUBSTANCE: invention refers to medicine, particularly to vascular surgery and can be used in experimental surgery. It involves an extensive sympathectomy of the common, internal and external carotid arteries by removing their adventitial coats. The common carotid artery is fixed to a medial edge of the fascial compartment of the sternocleidomastoid muscle. At the level of occipital vessels between two ligatures, the external carotid artery is transected, and within a bifurcation point of the common carotid artery, two clamps are applied on the internal carotid artery. The lower clamp is placed along an extension line of a lateral edge of the external carotid artery, while the upper clamp - transversally and in between; the internal carotid artery is transected; anastomosis of a distal end of the internal carotid artery with a proximal end of the external carotid artery is created, and pathological sinuation of the internal carotid artery is formed.
EFFECT: method extends the range pf products for simulating pathological sinuation of the internal carotid artery in experimental animals.
SUBSTANCE: for simulation of non-alcoholic steatohepatitis in rats 4.25 g of melted beef fat and 0.43 g of powdered cholesterol per 100 g of body weight were administered to animals daily during 6 months with food. The method provides an adequate reproduction of the model.
EFFECT: proposed model is simple in execution, shows good reproducibility, lack of death in experimental animals and complexity in forming dismetabolic violations in liver and body as a whole typical for this disease.
4 tbl, 1 dwg
SUBSTANCE: for simulation of chronic pancreatitis destruction of pancreatic tissue of rats is carried out with 1% solution of Triton X-100 in the amount of 0.1 ml. After a single injection of the drug to experimental animals they are given 15% aqueous ethanol to drink instead of water during three months.
EFFECT: high reproducibility of chronic inflammation in pancreas in rats, maximally approaching this pathological process to that of humans, and simplicity of the method in use.
SUBSTANCE: coefficient exponent of reoophthalmography are daily measured in a healthy eye and an injured eye. If coefficient exponents of reoophthalmography in the injured eye is 1.1-1.6 ‰ lower than in the healthy one, the state is estimated as an acute stage of wound process; if coefficient exponents of reoophthalmography in the injured eye is 0.1-1.0 ‰ lower than in healthy it is estimated as subacute stage of wound process.
EFFECT: method enables to identify stage of wound process in due time, to conduct abolition of antibiotics and sulphonamides in required timeframe, to reduce period of epithelialisation and treatment for 5-10 days.
1 dwg, 1 tbl, 2 ex
SUBSTANCE: percutaneous puncture of liver is carried out at ultrasound control. A dual-channel catheter with inflatable rubber balloon is inserted through the acus. The balloon is inflated to the desired size of the simulated cavity through the first channel. A sclerosing solution is inserted intraparenchymatously through the second catheter channel. On the 4-5th day the balloon is emptied. X-ray and ultrasonic inspection of the cyst is carried out.
EFFECT: required volume of the simulated cyst, ability to control dynamically the cyst parametres, to reduce period of fibrous capsule formation, less traumatic.
1 dwg, 1 ex
SUBSTANCE: for 10 days, UltrasorbR in amount 3 g a day supplements a diet of an experimental animal with simulated acute poststreptococcal glomerulonephritis. It is combined with intraperitoneal introduction of Amoxiclav in dosage 100 mg/kg.
EFFECT: preserved glomerular apparatus, reduced albuminous degeneration of epithelial cells.
FIELD: medicine, experimental abdominal surgery.
SUBSTANCE: as experimental animals one should apply mongrel dogs of 12-17 kg body weight. Under general anesthesia one should conduct superior-median laparotomy, introduce 3.0 ml 70%-ethanol solution under pancreatic capsule and then laparotomic wound should be sutured up. Manipulation should be performed once. The method provides modeling adequate acute pancreatic inflammation at no side effects being very simple in implementation.
EFFECT: higher efficiency.
FIELD: medicine; medical engineering.
SUBSTANCE: method involves studying transverse longitudinal and rotation stiffness characteristics. The studies are carried out step-by-step from the first order units to complete external fixation apparatus structure. The device has frame and is provided with calibration loads, wire rope, displacement indicators, strip for fastening to loading end of bone imitator fragment, beam for fixing displacement indicators, beams having unit for modeling longitudinal and transverse loadings. The frame is manufactured as parallelepiped. The fixing panel has openings for bone imitator, for fixing external fixation apparatus and yoke connection union and is fixed in end face part of the frame. Beam for fixing displacement indicators has longitudinal slit for fixing the indicators and arranging them on lateral slots in frame base. The beams having unit for modeling rotational, longitudinal and transverse loadings are arranged on lateral frame sides on lateral slots in base.
EFFECT: high vision acuity without applying spectacle-based correction; accelerated treatment course.
2 cl, 16 dwg, 1 tbl
FIELD: experimental medicine.
SUBSTANCE: the present innovation deals with modeling urinary calculosis in rats due to injecting intraperitoneally 60%-glucose solution at 1 ml/100 g animal body weight twice daily for 2 mo. The method is very simple and enables to achieve lithogenesis in 25% experimental animals.
EFFECT: higher efficiency of experimental modeling.
2 dwg, 2 ex, 4 tbl
SUBSTANCE: method involves using Hann diode crystal with proper frequencies of pathogenic microorganisms and cells during their death period or during the stimulating factors action period being applied.
EFFECT: enhanced effectiveness of treatment; wider range of biophysical action types.
3 cl, 1 tbl
FIELD: medicine, experimental physiology.
SUBSTANCE: hypoxia with hypercapnia should be modeled due to creating a closed system of inhaled air circulation. Air enters lungs out of hermetically sealed reservoir and at expiration returns back. The process of recirculation is supplied with an apparatus of artificial pulmonary ventilation. The innovation suggested provides steadiness in development of hypoxia with hypercapnia excluding the development of stressor reaction. Conditions should be created to carry out any manipulations with an animal in the course of an experiment.
EFFECT: higher efficiency of modeling.
5 dwg, 1 ex
FIELD: medicine, stomatology.
SUBSTANCE: the present innovation should be carried out for the purpose to study ethiology and pathogenesis of parodontitis. One should affect with emotional stress in experimental animals (mature rats) due to placing 10-11 experimental animals into the cage at area of 0.018 sq. cm/animal. Before placing into the cage one should create artificial dental plaque around the cervix of the upper and lower incisors with the help of stomatological cement for every experimental animal. In the course of modeling all experimental animals should eat paste-like food. The method enables to shorten terms for obtaining the model desired and increase its similarity with pathomorphological manifestations of human parodontitis.
EFFECT: higher efficiency of investigation.
FIELD: medical equipment.
SUBSTANCE: device has input first variable resistor, capacitor and permanent resistor. Permanent resistor is connected to arm second and third variable resistors. Second ends of variable resistors are connected with motionless contacts of polarized relay. Movable contact of relay is connected to common bus. Input of device is connected to amplifier which has output connected with control wiring of polarized relay. Second end of wiring is connected with common bus. Device is intended for electrical modeling of balanced and misbalanced conditions of acupuncture point at electropunctural action with unlike-poled signals due to liquidation mutual errors at any circuit of opposite arms of the device. Values of active resistances can be installed independently at any arm of device.
EFFECT: increased precision.
FIELD: experimental medicine.
SUBSTANCE: laboratory animals should be once injected intraperitoneally or intravenously with phenylhydrazine at the dosage of 100-150 mg/kg.
EFFECT: higher efficiency.
1 cl, 4 ex, 2 tbl
FIELD: medicine, experimental biology, ecology, toxicology.
SUBSTANCE: at studying the mechanisms of heavy metals toxic action, in particular, cadmium upon renal function, it is suggested to introduce cadmium sulfate solution into stomach once daily for 2 mo at the dosage of 0.5 mg/kg, on conversion to metal, where cadmium corresponds to 0.5 mg per 1 ml solution. The present innovation enables to study the pathology in dynamics of development and elaborate and searching preparations for treating and preventing chronic toxic nephropathy.
EFFECT: higher efficiency.
1 ex, 3 tbl
SUBSTANCE: method involves exposing cell or cell group to external power source. At least two electrodes are introduced before treating the cells. One of electrodes is set on cytoplasmatic external cell membrane surface and the other one cell membrane and membrane potential value is measured. External electric voltage source is connected to the introduced electrodes oppositely in polarity with cell membrane potential difference value being not less than cell membrane potential.
EFFECT: enhanced effectiveness in building cell damage model by means of energy burst and death.