Method of separating surface component from fluid heterogeneous medium

FIELD: process engineering.

SUBSTANCE: method is intended for use in biotechnology, particularly for separating surface component from fluid heterogeneous biotechnological medium. Proposed method consists in shaping aerosol by ultrasound from fluid medium surface layer enriched by component to be extracted, forcing it out by airflow and condensing it in fluid with increased content of extracted component.

EFFECT: continuous process, preventing suppression of activity of biosynthesising microorganisms by biosynthesis products, reduced power consumption.

10 ex

 

The invention is intended for use in biotechnology, in particular for the allocation of surface components from a liquid heterogeneous biotech environment, for example from the mash, ethyl, butyl or other alcohol and some other organic compounds, with the aim to ensure the implementation of continuous process through continuous, does not violate biosynthesis, selection synthesized by microorganisms useful metabolite, for example, ethyl, methyl, butyl alcohols and other organic compounds from the environment of the cultivation of microorganisms; prevent suppression activity biosynthesised microorganisms products biosynthesis; reduce power consumption required for the selection of useful metabolites from heterogeneous environments.

A known method of separation of volatile metabolites of biotechnological environment by distillation under vacuum during biosynthesis [1]. However, the method is unsuitable for separation of metabolites with low pressure saturating vapor, or in the case of low concentrations of metabolites in a biotechnology environment. In addition, the consumption of the process is great, as is determined by the energy required to break all intermolecular bonds.

To achieve this objective, it is suggested once the bookmark to use that formation of aerosols from the surface layer of liquid biotech environment enriched surface component of the environment due to the known effect of surface adsorption [2].

Enriched secreted substance aerosols obtained using the ultrasonic generator aerosol, representing, for example, concave ultrasound emitters, emitting acoustic energy from a volume of liquid and focusing her near the interface liquid - gas. The droplet size of the aerosol is determined by the operating frequency of the atomizer and the coefficient of surface tension of the liquid and, as a rule, lie in the range from 1 to 50 micrometers [3]. The temperature of the partial fluid with virtually no increases and their contained components are not experiencing undesirable reactions, including due to the action of ultrasound with low average intensity [4].

Known methods of isolation, for example, alcohols of heterogeneous biotech environment include distillation - distillation through the vapour phase [5], the separation of the membranes [6, 7], the separation with sorbents [8], freezing [9]. All technologies based on the listed methods have the following drawbacks: high intensity, for example, or the need for relatively frequent replacement or restore the original properties of the functional elements.

The closest way to the same destination to declare the mu the invention by the combination of essential features is the method of distillation of liquid medium through the vapour phase with subsequent separation by condensation [5].

The present invention is directed to:

- reduction of energy consumption in the process of selection of the required components from a liquid heterogeneous environment

- elimination of temperature effects, in some cases dramatically change the properties of components biotech environment

- ensuring continuous separation of the useful metabolite in the process of biosynthesis, which can prevent the reduction in the activity of microorganisms

- elimination of the need for regeneration of filters, sorbents, and other functional elements.

This result is achieved by the fact that

- ultrasonic aerosol formed from the surface layers of the liquid medium, enriched by the phenomenon of surface sorption,

their education does not require energy to break all the bonds between molecules of a liquid, as by distillation through a steam phase. Energy is expended only on the gap smaller number of intermolecular bonds in the formation of droplets of fog,

- does not require increasing the temperature to values that ensure the boiling liquid, and does not change its components, in particular has no inactivating effect on microorganisms

- does not require periodic replacement or restoration of functional elements, membranes, sorbents and other, which then helps to ensure continuity of the process.

Thus, the set of distinctive features of the described method ensures the achievement of a specified result.

As a result of the analysis of the prior art scroll to the desired component of the biotech media source, characterized by signs, identical with all the essential features of the claimed invention, not found, therefore the claimed invention meets the condition of "novelty."

Additional search known solutions showed that the claimed invention not apparent to the expert in the obvious way from the prior art, since the proposed method possesses a number of characteristics that reduce energy consumption in the process of selection of the required components from a liquid heterogeneous environment, eliminates the effects of temperature, in some cases dramatically change the properties of biotechnological environment provides conditions for continuous separation of the useful metabolite in the process of biosynthesis, which can prevent the reduction in the activity of microorganisms, there is no need for periodic regeneration of functional elements (filters, membranes, sorbents). Therefore, the claimed invention meets the condition of "inventive step".

Information confirming the possibility Rea is Itachi of the invention to provide the above technical result

Check the possibility of realization of the invention was carried out on the device for producing ultrasonic aerosol, providing focusing of the acoustic energy emitter to the surface of the section of model samples of liquid and air and the formation of aerosol from the surface layer of the liquid enriched surface component due to the phenomenon of surface sorption.

Model fluid samples were prepared by mixing, for example, ethyl and/or butyl alcohols, and/or sugar, and/or glycerol, and/or sodium chloride with water in various ratios.

The resulting aerosol was caught in the apparatus with liquid shutter formed of the same condensed aerosol.

The content of the investigated substances concentrate with the present invention were determined by conventional methods, for example, gas chromatography, refractometry, polarimetry and other

The efficiency of the method depending on the conditions of its implementation was assessed by the ratio of the content focus of a substance in unit volume of the original model fluids containing the same substance in the liquid obtained by the condensation of an ultrasonic aerosol.

The essence of the invention is illustrated by the following examples.

Example 1

An aqueous solution of ethyl alcohol with a concentration of 5% is subjected to side fluid is phase action focused on the interface liquid - gas ultrasound intensity in the focal region, sufficient to transform the liquid into an aerosol. The resulting aerosol for the reverse transformation in the liquid divert the flow of air into a trap with a water seal. The ratio of the concentrations of ethanol, measured refractometric method in the source and obtained after the inverse transformation of the liquid, was 1.36.

Example 2

An aqueous solution of ethyl alcohol with a concentration of 10% is subjected from the liquid phase to the action focused on the interface liquid - gas ultrasound intensity in the focal region, sufficient to transform the liquid into an aerosol. The resulting aerosol for the reverse transformation in the liquid divert the flow of air into a trap with a water seal. The ratio of the concentrations of ethanol, measured refractometric method in the source and obtained after the inverse transformation of liquid was equal to 1.4.

Example 3

An aqueous solution of ethyl alcohol with a concentration of 35% is subjected from the liquid phase to the action focused on the interface liquid - gas ultrasound intensity in the focal region, sufficient to transform the liquid into an aerosol. The resulting aerosol for the reverse transformation in the liquid divert the flow of air into a trap with a water seal. the rate of concentration of ethyl alcohol, measured refractometric method in the source and obtained after the inverse transformation of the liquid is equal to 1.26 in.

Example 4

Water-salt solution of ethyl alcohol with an alcohol concentration of 10% and sodium chloride and 0.08% exposed from the liquid phase to the action focused on the interface liquid - gas ultrasound intensity in the focal region, sufficient to transform the liquid into an aerosol. The resulting aerosol for the reverse transformation in the liquid divert the flow of air into a trap with a water seal. The ratio of the concentrations of ethanol, measured refractometric method in the source and obtained after the inverse transformation of liquid was equal to 1.8.

Example 5

Water-salt solution of ethyl alcohol with an alcohol concentration of 10% and sodium chloride 0.2 per cent exposed from the liquid phase to the action focused on the interface liquid - gas ultrasound intensity in the focal region, sufficient to transform the liquid into an aerosol. The resulting aerosol for the reverse transformation in the liquid divert the flow of air into a trap with a water seal. The ratio of the concentrations of ethanol, measured refractometric method in the source and obtained after the inverse transformation of liquid was equal to 1.6.

Example 6

Water-salt solution of ethyl alcohol with an alcohol concentration of 10% and sodium chloride 1% exposed from the liquid phase to the action focused on the interface liquid - gas ultrasound intensity in the focal region, sufficient to transform the liquid into an aerosol. The resulting aerosol for the reverse transformation in the liquid divert the flow of air into a trap with a water seal. The ratio of the concentrations of ethanol, measured refractometric method in the source and obtained after the inverse transformation of liquid was equal to 1.3.

Example 7

Water-salt solution of ethyl alcohol with an alcohol concentration of 10% and sodium chloride 7% exposed from the liquid phase to the action focused on the interface liquid - gas ultrasound intensity in the focal region, sufficient to transform the liquid into an aerosol. The resulting aerosol for the reverse transformation in the liquid divert the flow of air into a trap with a water seal. The ratio of the concentrations of ethanol, measured refractometric method in the source and obtained after the inverse transformation of the liquid is equal to 1,06.

Example 8

An aqueous solution of n-butyl alcohol with a concentration of 0.5% is subjected from the liquid phase to the action focused on the interface liquid - gas ultrasound intensity in the focal region, sufficient to transform the liquid into an aerosol. The resulting aerosol for its reverse transformation to divert fluid flow is om air in the trap with a water seal. The concentration ratio of n-butyl alcohol, measured refractometric method in the source and obtained after the inverse transformation of liquid was equal to 3.2.

Example 9

An aqueous solution of n-butyl alcohol with a concentration of 1% is subjected from the liquid phase to the action focused on the interface liquid - gas ultrasound intensity in the focal region, sufficient to transform the liquid into an aerosol. The resulting aerosol for the reverse transformation in the liquid divert the flow of air into a trap with a water seal. The concentration ratio of n-butyl alcohol, measured refractometric method in the source and obtained after the inverse transformation of liquid was equal to 2.0.

Example 10

An aqueous solution of n-butyl alcohol with a concentration of 2% is subjected from the liquid phase to the action focused on the interface liquid - gas ultrasound intensity in the focal region, sufficient to transform the liquid into an aerosol. The resulting aerosol for the reverse transformation in the liquid divert the flow of air into a trap with a water seal. The concentration ratio of n-butyl alcohol, measured refractometric method in the source and obtained after the inverse transformation of liquid was equal to 1.8.

The composition of the medium: Water +The content in the initial solutionThe content in the obtained solutionThe ratio of the source and resulting concentrations
Ethanol5%6,81,36
Ethanol10%6,81,4
Ethanol25%6,81,26
Ethyl alcohol sodium Chloride10%
0,08%
16%1,6
Ethyl alcohol
Sodium chloride
10%
0,2%
18%1,8
Ethyl alcohol
Sodium chloride
10%
1%
13%1,3
Ethyl alcohol
Sodium chloride
10%
7%
10,5%1,05
Botilony alcohol 0,5%1,6%3,2
N-butyl alcohol1%2,1%2,1
N-butyl alcohol2%3,6%1,8

The examples illustrate that the transformation enriched surface component of the surface layer of fluid in the aerosol and its subsequent condensation in liquid lead to an increase in the content of the allocated components. This effect is most pronounced at low concentrations of the allocated components from the source environment that is explained by the rapid saturation of the surface layer of the source environment, for example, molecules of surface-active substances and it is almost constant concentration after completion of the process of saturation. Thus, the above data suggest that the claimed invention, intended for use in biotechnology, in particular for the allocation of surface components from a liquid heterogeneous biotech environment, has the above properties. For the inventive method, it is described in the above-mentioned formula of the invention, a confirmation is eridana the possibility of its implementation using the steps described in the application of tools and techniques. Therefore, the claimed invention meets the condition of "industrial applicability".

Literature

1. Eskow A.A., Arsen'ev A.I., Kuzmichev A. Vacuum technology in the production of fuel ethanol. Economic aspects // Production of alcohol and alcoholic beverages. - 2006. No. 4. - P.32-33.

2. Sheludko A. "Colloid chemistry". Translation from English. edited Bddesign and OSA. Moscow. Statenot, 1960 s-307.

3. "Ultrasound" Small encyclopedia. Chiefly. the drafting. Iphoneman. Ed."Soviet encyclopedia" Moscow - 1989, 399 pages

4. Hakobyan V.B. have been, Ershov Y.A. Basis of the interaction of ultrasound with biological objects (ultrasound in medicine, veterinary medicine and experimental biology). - M., Izd-vo RGTU. Bauman, 2005, 300 S.

5. Kasatkin A.G. Basic processes and apparatus of chemical technology, 8 ed., M., 1971.

6. Hwang, S.-T., Kammermeier K. Membrane separation processes. - M.: Chemistry, 1981.

7. V.A. Shaposhnik Membrane electrochemistry // Soros Educational Journal. 1999. No. 2. P.71-77.

8. Yu.A. Zolotov, Kuz'min N.M. Preconcentraion in Inorganic Analysis. Amsterdam: Elsevier, 1990.

9. Dads L., Concentrating by freezing, M., 1982.

The method of allocation of surface components from a liquid heterogeneous environment, characterized by the fact that rich allocated component of the surface layer of a liquid medium under the action of ultrasound undoubtedly the comfort aerosol, after which it take away the air flow and condense into a liquid with a high content of allocated components.



 

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