Method of detecting lifetime paranecrotic change of tissues and skeletal system in animals
SUBSTANCE: to detect the lifetime paranecrotic changes of tissues and skeletal system in experimental animals, average weight molecules in blood plasma are evaluated by spectrophotometric analysis in the wave length range 250-300 nM. The derived data are estimated by the area of a figure formed by an absorption curve and a zero mark axis. If the average weight molecules are increased in relation to the initial level in the wave length range 250-270 nM, the lifetime paranecrotic changes are observed.
EFFECT: method allows detecting the paranecrotic changes in tissues and skeletal system of experimental animals without killing.
1 ex, 1 tbl
The invention relates to the field of experimental medicine and can be used for modeling pathological reactions, processes, and States to determine in vivo parametricheskikh changes in the tissues of the organs and bones of experimental animals.
Parametricheskii are the changes in the tissues, which may, depending on conditions go to the irreversible stage of necrosis or be restored to its original state (1). It is known that parametricheskie processes increase in the structures of the nervous tissue and other organs under different effects on the organism of experimental animals (2), as well as disorders of endocrine regulation (3), which are accompanied by increased sorption vital dyes.
Still in experimental medicine structural and functional changes in tissues and organs revealed after the killing of animals, studies of biopsies of suspected damaged organs (4). There are also chemical method for detection of in vivo changes in the tissues of the organs by examining the chemical composition of the blood (5) and histochemical evaluation method for in vivo parametricheskikh changes in the tissues (6). However, these methods have several disadvantages associated with the necessity of killing animals and traumatise the tion of the studied organs.
As a prototype for closest to the technical nature of our chosen the way histochemical determination parametricheskikh of life changes in the tissues of the organs, namely, that the number of adsorbed cloth bodies paint neutralist judge available parametricheskikh life changes (7), as this paint stains denaturation changes in the cell at a reversible stage (8).
The method is as follows. Experimental animal (in this case the rat) injected intraperitoneally 0.5% aqueous solution naturalrate in the amount of 0.25 ml per 100 g mass. Then after 30 min the animal is slaughtered and retrieve the bodies, which are placed in loireau environment (acidified with concentrated sulfuric acid solution 70° ethyl alcohol) in an amount of 5 ml per 1 body. After 24 hours the eluate photometrate to determine the adsorbed dye cloth bodies. To assess the tissue distribution of the ink bodies are placed in the incubator for 24 hours at 37°. The result is appreciated in mg per gram dry tissue.
This method for determining parametricheskikh life changes has the disadvantage associated with the necessity of killing animals for subsequent extraction of the dye from the tissues of the organs, and, therefore, there is no opportunity to study in vivo the pair is eroticheskih changes in the tissues of the organs and bones of experimental animals in the dynamics.
The technical result of the invention is the possibility of studying in vivo parametricheskikh changes in the tissues of the organs and bones of the experimental animal in the dynamics, as well as the definition of lifetime parametricheskikh changes in the tissues of the animal without killing.
The technical result is achieved by the fact that in experimental animals to determine the average molecule mass (MSM) in the range of 250-300 nm in plasma and when they increase from baseline in the range of 250-270 nm wavelengths determine the in vivo changes in the tissues of the organs and bones.
The method is as follows:
Plasma (0.5 ml) experimental animal according to the method Myamalfoy (8) determine the MSM. Blood samples of 1 ml is carried out with decapitate or from the tail vein (in rats). The deposition of macromolecular proteins spend 15% solution of trichloroacetic acid (0.5 plasma 0.5 ml of 15% R-RA trichloroacetic acid). Clot denaturirovannykh proteins stir with a glass rod, and then centrifuged at 5000 revolutions per minute. Then 0.5 ml of centrifugate add 9.5 ml of distilled water and photometrate spectrometer at wavelengths from 250 to 300 nm. The obtained results are judged by the size of the square shape formed by Krivovichev absorption and the axis zero point. Express this value in arbitrary units. Then experiment, which consists in the simulation of pathological reactions, processes and States. In this case, used a model of traumatic effects on the animal body by mechanical fracture of the bones of the leg. 24 hours after the injury examined the blood contains MSM and when they increase from baseline in the range of 250-270 nm wavelengths determine the in vivo changes in the tissues of the organs and bones of the experimental animal.
Distinguishing the essential feature of the proposed method is the determination in experimental animals of molecules of average weight (MSM) in plasma and when they increase from baseline in the range of 250-270 nm wavelength determination of in vivo changes in the tissues of the organs and bones.
The causal link between distinguishing the essential feature of the proposed method and achieve the result:
As is known, the impact of many pathogenic factors may cause significant changes in neuroendocrine regulation with subsequent disruption of the internal environment and the presence of several humoral factors endogenization pathological processes (9). While involved in the pathological process and is not damaged body is (in this case not injured organs), exacerbating the course of the basic pathological process. Currently, among the factors of endogenous intoxication of paramount importance MSM (10), which are represented by the classes of compounds with a molecular weight of up to D. The range of 250-270 nm wavelengths, this method for determining the in vivo changes in the tissues of the organs and bones of the experimental animal, chosen by the authors experimentally.
We first established correlative relationship between increased from baseline by MSM, defined in the spectrum of wavelengths from 250 to 270 nm, and lifetime parametricheskii changes in the tissues of internal organs and bones of the experimental animal.
Distinctive significant feature of the proposed method is new and allows to determine in vivo parametricheskie changes in the tissues of the animal without killing that makes it possible to study in vivo parametricheskie changes in the tissues of the organs and bones of the experimental animal in the dynamics.
(Rat No. 3A and 4A of Protocol No. 7). Experiment: a fracture of the right ankle under General anesthesia. Study after 18 days on the prototype method and the proposed method.
In rat No. 3A of hvos the OIC Vienna was taken blood and investigated by the present method for the determination of plasma MSM in the range of 250-300 nm, used spectrophotometer. It was found that in the range of 250-270 nm MSM totaled 33.7 USD, while the control rats, this indicator was equal to 10.8 cu, indicating in vivo parametricheskikh changes in the tissues of the organs and bones of the rat No. 3A.
In rat No. 4A from the tail vein was also taken blood and investigated by the present method for the determination of plasma MSM in the range of 250-300 nm. It was found that in the range of 250-270 nm MSM reached 8.9 cu, in control rats, this indicator was equal to 10.8 cu, indicating the absence of in vivo parametricheskikh changes in the tissues of the organs and bones of the rat # 4A.
The obtained data confirmed the results obtained in the study of in vivo parametricheskikh changes in the tissues of the organs and bones of the rat according to the method prototype:
|Bodies||The content of paints mg per 1 gram of tissue|
|Control||Rat 3A||Rat 4A|
The essence of the method is illustrated by results obtained in rats with fractures of tubular bones (table - Comparative table of the evaluation of life parametricheskikh changes in tissues and organs of the skeletal system in rats with experimental fracture of the Shin bone on the prototype method and by the present method).
The data presented in table 1, indicate that in vivo parametricheskie changes in the tissues of rats, determined by the method prototype, identified using the proposed method.
Thus, the inventive method can be used to detect in vivo parametricheskikh changes in the tissues of the organs and bones of experimental animals without killing them, which gives the opportunity to study in vivo parametricheskikh changes in the tissues of the organs and bones of experimental animals in the dynamics.
|Comparative table of the evaluation of life parametricheskikh changes in tissues and organs of the skeletal system in rats with experimental fracture of the Shin bone on the prototype method and the present method|
|The follow-up period||CONTROL (n=9)||After 24 hours (n=8)||After 18-21 days (n=7)|
|Molecules average||11.3+2.7 $||25.2+3.4 $ *||9.8+3.7 $|
|mass (cu - conditional units)||(250-270 nm)||(250-270 nm)||(250-270 nm)|
|31.5+3.1 $||17.8+ $ 5.4||29.6+3.2 $|
|(the inventive method)||(280-300 nm)||(280-300 nm)||(280-300 nm)|
|Note: * - significant change (p<0.05)|
Sources of information
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2. Novels S.N. The biological effect of mechanical vibrations. - L., 1983. - 208 S.
3. Khegay PPM Pathophysiological basis for the development of complications in insulin-dependent diabetes mellitus. - Abstract. Diss. for obtaining the academic degree of doctor. the honey. Sciences. - SPb., 1998. - 39 S.
4. Fingers M.A., Anichkov NM Pathological anatomy. - M., 2000. Vol. 1. C.11-23.
5. Homolo PS Neurogenic atherosclerosis and lipidosis aorta. - M., 1972. S-247.
6. Gramenitskiy E.M. Intravital staining cells and tissues. - Leningrad, 1963. - 149 C.
7. A.S. Troshin the Distribution of substances between the cell and the environment. - L., 1985. - 192 S.
8. Medical laboratory technology. Handbook / edited Aeacides, Vol.2. - St. Petersburg, 2002. 400 S.
9. Kryzhanovsky GN. Introduction to General pathology. - M., 2000. - 71 S.
10. Kopytova T.V. molecules of average weight as the substrate of endogenous intoxication in severe dermatoses // Successes of modern natural science. 2006. No. 9. P.7-10.
The method of determining the lifetime parametricheskikh changes in tissues and organs of the skeletal system the experimental is the rest of the animals, characterized in that to determine the average molecule weight in blood plasma by spectrophotometry in the range of 250-300 nm wavelengths, with the results evaluated according to the magnitude of the square shape formed by the curve of the magnitude of the absorption axis and the zero mark, and with the increase of molecules of average weight with respect to the initial level in the range of 250-270 nm wavelength, determine the lifetime parametricheskie changes.
SUBSTANCE: before and after local ischemia, diametre of the brachial artery, blood nitrite level, endothelin concentration and Willebrand factor are evaluated. The accommodation of vascular wall endotheliocytes to ischemia is calculated by formula that describes a coefficient Ken. If Ken is "0" or less, the adaptation of the vascular wall is considered as preserved; Ken within "0" to 0.2 inclusive shows the moderately lowered adaptation of the vascular wall, while the Ken value exceeding 0.2 indicates the considerably lowered adaptation of the vascular wall. The coefficient Ken may be used for a quantitative estimation of specific features of cell functional adaptation to ischemic factors, both in norm, and in cardiovascular pathology.
EFFECT: use of the invention allows reducing the acquisition time in 3 times, objectively assessing the endothelium adaptation to ischemia.
SUBSTANCE: invention refers to medicine, particularly to morphology and histochemistry. Computer analysis is implemented by photography of histological drugs processed by Cross-Ewen-Rost method with orthophthalic aldehyde, and photography assessment. Digital photography is applied, and histamine concentration is determined at software level by mean brightness of an digital image site measured in reference luminosity units within 0 to 255 in graphic editors.
EFFECT: extended range of histamine concentration determination methods for biological structures.
4 dwg, 1 tbl
SUBSTANCE: invention belongs to experimental and clinic medicine, notably to medical biophysics, clinic pharmacology, biochemistry, oncology and immunology. For antioxidant activity assessment, test specimen of an oxidant is injected into biological target, and then in target free-radical oxidation is induced. Antioxidant activity is assessed by optical density of the patterns within photometry. As biological target whole blood with addition of citrate or heparin is used. Test specimen of an oxidant is injected in 5% suspension made of whole blood, after incubation 10 mcl of 1 mM clotrimazole suspension is added. To induce oxidation 20 mcl of 100 mM tert-butyl hydroperoxide solution is added. Mix is once again incubated, centrifuged, and then supernatant's optical density is measured.
EFFECT: method allows increasing of accuracy and effectiveness of antioxidant activity assessment in natural and chemical compounds and biological liquids.
6 cl, 2 dwg
SUBSTANCE: invention belongs to medicine, notably to morphology and hystochemistry. In this method of histamine rate assessment in epithelial cells of rats endometrium within sex cycle, sample acquisition and following fluorescent-histochemical analysis with orthophtalic aldehide is made. In each phase of sex cycle histamine rate assessment in vaginal content is made. Assessment's result is compared with rate of histamine which is 0.50±0.07 at early estrus stage, 0.34±0.04 at late estrus stage, 0.68±0.08 at metaestrus stage, 0.72±0.12 at early diestrus stage, 0.75±0.13 at late diestrus stage, 0.87±0.11 at proestrus stage. Measurement unit is conventional unit of measuring device. When rate of histamine in vaginal content is below control value the rate of histamine in epithelial cells of endometrium is assessed as reduced, if it exceeds control value same does the histamine rate in endometrial cells.
EFFECT: improvement of evaluation accuracy.
2 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely to forensic medicine, and can be used for late postmortal detection of prescription of death coming. The optical density of vitreous body is measured. The prescription of death coming is calculated provided a corpse kept at an ambient temperature +10°C to +20°C by formula: PDC=1.975+5.641×OD335+13.924×OD405-16.023×OD475+6.938×OD545, where PDC is the prescription of death coming, (days); OD335 is the optical density of vitreous body at wavelength 335 nm; OD405 is the optical density of vitreous body at wavelength 405 nm; OD475 is the optical density of vitreous body at wavelength 475 nm; OD545 is the optical density of vitreous body at wavelength 545 nm, and at ambient temperature +21°C to +30°C by formula: PDC=1.129+136.486×OD365-134.091×OD375+10.269×OD405, where PDC is the prescription of death coming, (days); OD365 is the optical density of vitreous body at wavelength 365 nm; OD375 is the optical density of vitreous body at wavelength 375 nm; OD405 is the optical density of vitreous body at wavelength 405 nm.
EFFECT: method is accessible, simple and characterised by high accuracy.
SUBSTANCE: tumour region and surrounding intact tissue receives the diagnostic exposure prior to the series low-intensity lasing source exposure that is carried out at average radiation power 1-10 mWt, wavelength 370 nm with recording the tissue-escaping intensity of back-scattered fluorescence at wavelengths 420, 460 and 520 nm. Thereafter, the tumour region and the surrounding intact tissue are exposed to the low-intensity lasing fluorescence at wavelength 532 nm with recording the tissue-escaping intensity of back-scattered fluorescence at wavelengths 560 and 608 nm. Then the immunohistochemical analysis additionally precedes the treatment with recording the Ki-67 antigen expression level of that tumour region exposed to the low-intensity lasing to determine a prediction criterion P1 by the initial Ki-67 antigen expression level; there is evaluated the relation of fluorescence intensities at wavelengths 420/460 nm, 460/520 nm, 560/608 nm derived from the tumour surface Ii:K2=I420/I460, K3=I460/I520, K4=I560/I608 and in the intact region I'i:K'2=I'420/I'520, K'3=I'460/I'520, K'4=I'560/I'608 to calculate the relation of the tumour/intact tissue fluorescence intensity at wavelengths 420/460 nm, 460/520 nm, 560/608 nm: K2/K'2, K3/K'3, K4/K'4; if the Ki-67 antigen expression > 65%, the prediction criteria P1 are considered to be equal to +1, the expression 45-65% shows P1=0, the expression <45% requires P1=-1; the prediction criteria P2, P3, P4 are derived from the results: P2, P3, P4=+1 respectively at K2/K'2<1, K3/K'3<1, K4/K'4<0.5, P2, P3, P4=0 respectively at K2/K'2=1-4, K3/K'3=1-1.4 K4/K'4=0.5-1.5, P2, P3, P4=-1 respectively at K2/K'2>4, K3/K'3>1.4, K4/K'4>1.5, while the radiation sensitivity is determined by total prediction criteria, and P1+P2+P3+P4>0 indicate high radiation sensitivity, P1+P2+P3+P4=0 ensures moderate radiation sensitivity, while in P1+P2+P3+P4<0, low radiation sensitivity is observed.
EFFECT: method simplifies prediction of the results prior to the therapeutic course by tumour sensitivity to ionising radiation, improves its reliability that allows correcting the management program.
1 tbl, 3 ex
SUBSTANCE: patient's blood serum is tested by infrared spectroscopy. It involves recording peak heights of absorption bands in the range 1170-1025 sm-1, evaluating absorption spectrum, expressing the related values as the regions of "disease patterns" in a coordinate system (x; y) with the coordinates: (x // y): -1.8325/2.2800; -1.3175/2.8650; -1.1468/1.7903; -1.1887/1.3774; -1.0694/0.4032; -1.8500/0.4000; -1.8242/1.6452 - showing clinical effectiveness, and the coordinates: (x // y): 1.1575/2.1000; -1.1274/1.7774; -1.1726/1.3710; -1.0575/0.4200; -0.3775/0.8900 indicating lack of effect.
EFFECT: more accurate and accelerated discovery of estimation of clinical effectiveness.
1 dwg, 2 ex
SUBSTANCE: blood plasma is analysed for cytochrome oxidase activity; concentrations of 2,3-diphosphoglycerate and lactic acid in erythrocytes are evaluated. Then oxygenation coefficient K is calculated by formula: K = (C1+C2):A, where A is plasma cytochrome oxidase activity, mol/l; C1 - concentration of 2,3-diphosphoglycerate in erythrocytes, mol/l; C2 - concentration of lactic acid in erythrocytes, mol/l. If said oxygenation coefficient is more than 1.0, hypoxia is diagnosed.
EFFECT: technique allows improving reliability of hypoxia diagnostics and reducing acquisition time to 4 hours.
SUBSTANCE: inventions are related to medical diagnostics and may be used to detect concentration of analysed substance, such as glucose, cholesterol, free fatty acids, triglycerides, proteins, ketones, phenylalanine or ferments, in physiological liquid, such as blood, plasm, saliva, urine, interstitial and/or intracellular liquid. Test -element includes the first and second surfaces, which are located at a distance from each other. Surfaces have substantially two identical and mainly congruently matched patterns, which create sections with high and low surface energy on the first and second surfaces, which form system of sample distribution at least with two sensitive sections for measurements. Physiological liquid is held by sections with high surface energy. Device versions are disclosed for performance of analysises, which comprises multiple test-elements, as well as method for making of test-elements, system of analysis for detection of analysed substance concentration in physiological liquid and method for detection of concentration of at least one analysed substance in sample.
EFFECT: provision of simple means and methods for analysis with application of small volume amounts of physiological liquid.
43 cl, 19 dwg
SUBSTANCE: invention concerns medicine and can be used in dermatology for diagnostics of psoriasis. To implement the diagnostic technique, clinical signs of psoriasis are determined, and as an additional laboratory criterion, spend an chemiluminescence (CL) of low-density and very low-density lipoproteid suspension recovered from the patient's blood serum. It is ensured by measuring sum of light - S, fast flash intensity - I and calculating the coefficient H equal to S to I relation. If H is more than 4.8, psoriasis is diagnosed for the examined patient.
EFFECT: application of the invention allows for objective diagnostics of psoriasis.
2 tbl, 1 ex
FIELD: medicine, analytical biochemistry.
SUBSTANCE: invention relates to laboratory methods of investigations. Method involves sampling specimen from patient to be inspected, extraction of serotonin and histamine from a specimen, chromatography of extract and determination of concentration of serotonin and histamine by the fluorescence intensity value. Saliva is used as biological fluid. Saliva by volume 1 ml is extracted with 4 ml of 1 N hydrochloric acid solution, 2 g of anhydrous potassium carbonate and 5 ml of mixture of butanol and chloroform in the ratio 3:2 are added, extract is shaken up and centrifuged. Organic phase (4 ml) is sucked off from extract and passed through chromatography column (diameter is 3 mm, height is 16 mm) filled with ion-exchange resin KB-4 or KB-4P-2 or Bio Rex-70 in H+-form, size of granules is 0.1 ± 0.02 mm. Histamine is eluted with 4 ml of 0.1 N hydrochloric acid at the rate of eluting solution 0.4 ml/min. Histamine concentration is determined by reaction with ortho-phthalic aldehyde dissolved in ethanol. Serotonin concentration is determined by reaction with ninhydrin in organic passed through column. Method provides assaying the saliva concentration of serotonin and histamine with high precision.
EFFECT: improved assay method.
SUBSTANCE: method involves studying blood samples with venous blood mixed with vital stain like methylene blue. Degree of vital stain absorption by erythrocytes is determined by applying photocolorimetry. The value drop being more than 25%, extracorporal detoxication is to be predicted as ineffective.
EFFECT: simplified method.
FIELD: medicine, infectology.
SUBSTANCE: one should detect the level of terminal stable metabolites of nitrogen oxide (NOx) in whole blood. At its value ranged 39.6-86.0 mcM/l one should evaluate hemorrhagic fever accompanied with renal syndrome (HFRS) of average severity, at NOx value ranged 86.7-141.5 mcM/l - severe form of HFRS and at its values ranged 88.2-128.6 mcM/l at the background of pronounced clinical picture - as complicated disease flow. The method enables to shorten the terms for carrying out the assays.
EFFECT: higher accuracy of evaluation.
3 ex, 1 tbl
FIELD: medicine, biochemistry.
SUBSTANCE: in blood serum one should detect the level of lactoferrin and biliary acids. At their ratio being equal to 5-17 it is necessary to detect chronic hepatitis of high activity.
EFFECT: higher accuracy of detection.
FIELD: medicine, dermatology, clinical laboratory diagnostics.
SUBSTANCE: the present method deals with detecting the focus of neutrophilic phagocytic activity lesion in capillary blood. At the values of cells' capacity to phagocytosis in percentage and phagocytic number on the 10th d of therapy being below 20% and 3.3, correspondingly one should evaluate therapeutic efficiency to be low, if it is above 40% and 4.0, correspondingly - as high.
EFFECT: higher accuracy of evaluation.
2 ex, 3 tbl
SUBSTANCE: method involves studying blood serum, processing obtained data and setting disease diagnosis. The study is carried out by preparing dried blood serum sample as suspension in Vaseline oil and doing the infrared spectroscopy analysis in the bandwidth of 120-1000 cm-1 and determining absorption strip peak heights having maximum at 1180; 1165; 1160; 1150; 1130; 1070; 1025 cm-1 and then calculating the following two ratio groups, the first of which is ratio of peak height with maximum at 1165 cm-1 to 1150 cm-1; 1160 cm-1 to 1130 cm-1; 1070 cm-1; 1025 cm-1. The second group has ratio peak having maximum at 1165 cm-1 to 1160 cm-1; 1180 cm-1 to 1130 cm-1; 1065 cm-1; 1070 cm-1. The obtained three-dimensional distribution of the first group is projected to frontal plane for calculating two-dimensional coordinates and comparing to flat reference diagnostic images of hepatic pathologies and to a normal reference diagnostic image represented as flat polygons which boundaries are given by the following values. The norm is represented by X(-2.3;2.0;4.0;4.0) and Y(1.6;0.8;0.8;1.6), respectively. Oncology is represented by X(1.7;1.7;0.0;0.0) and Y(1.9;1.25; 1.25;1.9). Hepatites are represented by X(1.9;2.2;1.8;1.4 and 1.9;1.8;4.0) and Y(1.9;1.9; 0.5;0.5 and 08;0.5;0.8). Cirrhosis is represented by X(1.9;2.6;1.4) and Y(1.6;0.8;0.4). Diseases are differentiated by interpreting point position within particular area. Three-dimensional distribution of the second group is projected to frontal plane and compared to diagnosis images of pathology and norm. Coordinate values of the second group are as follows: norm - X(1.8;2.9;2.5;1.5), Y(2.7;2.0;1.2;1.6); oncological cases - X(0.27;0.67;0.63), Y(0.27;0.67;0.3); hepatitis - X(1.5;2.5;2.4;1.2), Y(1.6;1.2;0.2;0.9); cirrhosis - X(1.1;0.9;0.9). Final diagnosis of pathology is set when particular data values belong to the corresponding pathology zone in both cases.
EFFECT: high accuracy of diagnosis.
SUBSTANCE: method involves studying biological material by applying infrared spectroscopy techniques. The obtained data are processed and diagnosis is set. Blood serum is used as the biological material. The study is carried out by preparing dried blood serum sample as suspension in Vaseline oil and doing the infrared spectroscopy analysis in the bandwidth of 120-1000 cm-1 and determining absorption strip peak heights having maximum at 1170; 1165; 1160; 1150; 1140; 1060; 1050; 1040; 1025 and then calculating the following ratio values like peak height with maximum at 1160 cm-1 to 1140 cm-1; 1165 cm-1 to 1150 cm-1; 1040 cm-1 to 1025 cm-1. The obtained distribution of this group is projected to frontal plane for calculating two-dimensional coordinates and comparing to flat reference diagnostic images of prostate pathologies and to a normal reference diagnostic image represented as flat polygons which boundaries are given by the following values. The norm is represented by X(-1.15;-0.9;0.45;0.0;-0.65) and Y(0.99;4.2;0.9;0.46), respectively. Pathology by X(-1.15;-1.15;0.35;0.0;0.65) and Y(0.99;-0.03; 0.48;0.09;0.46). The norm and pathology are differentiated. Additional mathematical processing is carried out on infrared spectra of blood serum samples of patients belonging to pathology image according to parameter values. First of all, three-dimensional distribution is calculated as peak having maximum at 1160 cm-1 to one having maximum at 1150 cm-1; 1170; 1160 cm-1; 1160 cm-1 to 1025 cm-1. It is projected then to frontal plane and compared to diagnosis images of prostate adenoma and images of prostate carcinoma. The second group relationships the following values are used: oncological cases - X(0.28;0.77;1.24;0.96), Y(0.75;0.46;-0.13;-0.02); adenoma - X(0.28;1.24;2.21;1.24;0.77), Y(0.75;1.24;-0.12;-0.13;0.46). Differential diagnosis of pathologies is set by interpreting point position within particular pathology image.
EFFECT: high accuracy of differential diagnosis.
SUBSTANCE: method involves determining mean cytochemical coefficient of lipid accumulation in peripheral blood leukocytes in conditional units before beginning therapy application (MCC1) and in 2-3 or 5-6, or 10-12, or 20-24 months of therapy application (MCC2). Therapy effectiveness coefficient is calculated in conditional units from formula K= MCC2/MCC1. The value being equal to or greater than 1, leprosy therapy is predicted to be effective.
EFFECT: simplified prognosis method.
1 dwg, 1 tbl
SUBSTANCE: method involves determining infrared radiation absorption coefficient in blood plasma in bandwidth of 1543-1396 cm-1. The infrared radiation absorption coefficient is determined in %. The value being equal to 29.7±1.1%, catarrhal cholecystitis is diagnosed. The value being 26.4±1.4%, phlegmonous cholecystitis is diagnosed. The value being 21.2±1.8%, gangrenous cholecystitis is diagnosed. The value being equal to 18.6±0.5%, gangrenous perforated cholecystitis case is diagnosed. The value in norm is equal to 32.4±0.8%.
EFFECT: high accuracy and specificity of diagnosis.
SUBSTANCE: method involves pouring venous blood treated with heparin into five conic test-tubes in the amount of 0.1 ml. The first three of them contain 0.1 ml of non-colored latex suspension with particle size of 1.5 mcm, the fourth one contains 0.1 ml of medium 199 and 0.1 ml of 0.1% aqueous solution of tetrazole nitro blue, the fifth one contains .1 ml of latex suspension and 0.1 ml of 0.1% aqueous solution of tetrazole nitro blue. The first test-tube is incubated in thermostat for 5 min at37°C, the second one for 30 min, the third one for 1 h, the fourth and the fifth one for 40 min. Smears are prepared from 0.2 ml of incubation mixture on glasses and dried at 37°C, fixed in burner flame, stained with 0.1% aqueous solution of tetrazole nitro blue, repeatedly dried and studied with microscope under immersion with magnification of 90x10. Test results are evaluated from absorption activity in phagocytosis reactions in determining the number of phagocytes, phagocytic number, phagocytic integral index and phagocytosis rate values. Tetrazole nitro blue test response is determined by counting formazan-positive cell number, calculating cytochemical activity index and tetrazole nitro blue test stimulation index.
EFFECT: accelerated test; high accuracy and low cost of examination.
1 dwg, 3 tbl