Method for stimulating erythropoiesis in bovine animals

FIELD: medicine, veterinary science.

SUBSTANCE: invention refers to veterinary science. The method involves intramuscular introduction of cerebrospinal fluid in a single dose of 0.1 ml per 1 kg of live weight in a left shovel region preliminary exposed to ultraviolet rays during 15 min.

EFFECT: invention allows stimulating erythropoiesis in bovine animals.

2 dwg, 2 tbl, 2 ex

 

The invention relates to veterinary medicine and can be used to treat animals with nutritional anemia prophylaxis anemic States and correction anemic syndromes.

Known way to prevent anemia relatedactivity drugs, e.g. ferroaluminum with the subsequent introduction of additional solution containing glycinate zinc, glycinate copper and potassium iodide (RU # 2135173, IPC6AC 31/295, 24.11.1994,).

The known method eliminates the deficiency of iron and trace elements in animals with expensive drugs, however, it is difficult to use and ineffective erythropoiesis stimulating animals.

The technical result is to increase the stimulation of erythropoiesis in cattle and reducing the cost of their treatment.

The technical result is achieved in that the method is characterized by intramuscular introduction to the area of the scapula on the left side irradiated with ultraviolet rays for 15 minutes cerebrospinal fluid of cattle a single dose of 0.1 ml per 1 kg of live weight.

Cerebrospinal fluid of cattle (CSF, cerebrospinal fluid) is clear colorless liquid, is a specific secret of the nervous system and fills the ventricles of the brain, spinash the howling channel and odpowiednie space of the spinal cord and brain. It is formed in the glandular cells of the vascular plexus of the cerebral ventricles. Cerebrospinal fluid performs nutrient functions, and determines the amount of intracerebral pressure. The amount of cerebrospinal fluid of cattle 300-320 ml, lifetime, one animal, such as cattle, atlantization tanks you can choose 100-150 ml.

The composition of the cerebrospinal fluid of cattle is formed in the process of exchange of substances between the brain, blood and tissue fluids, including all components of the brain tissue. In the cerebrospinal fluid contains a number of biologically active compounds, in size comparable with nanoparticles: the hormones of the pituitary and hypothalamus, norepinephrine, dopamine, serotonin, melatonin, the products of their metabolism. Total protein in the cerebrospinal fluid contains up to 30 different factions; the bulk of it form the myelin and formed at its intermediate decay products, glycopeptides, lipoproteins, polyamine, protein S-100. Also cerebrospinal fluid contains lysozyme and various enzymes (acid and Alp, ribonuclease, lactate dehydrogenase, acetylcholinesterase, peptidase and others). Cerebrospinal fluid is rapidly absorbed after parenteral introduction, has no toxicity featured in the effective doses bactericidal and does not contain bacteria.

The method is as follows.

Cerebrospinal fluid in vivo receive cattle from atlantization tanks in quantities of 100 to 150 ml of an animal. Cerebrospinal fluid of cattle just before the introduction of animals irradiated with ultraviolet rays for 15 minutes to enhance its pharmacological activity. Then irradiated with ultraviolet rays cerebrospinal fluid of cattle injected into the biologically active points (shoulder area on the left side), which is responsible for blood, a single dose of 0.1 ml per 1 kg of live weight.

Example 1. Study of the efficiency of the method of stimulation of erythropoiesis in cattle was carried out on 20 heifers black-motley breed, age 2 months, body weight 90 kg, with a diagnosis of anemia, which formed the 2 experimental groups of 10 animals each.

Conditions of feeding and maintenance of animals of both groups were the same. Animals of each group were placed in separate cells.

Animals of the 1st group once, subcutaneously, in the area of biologically active points, which is responsible for blood, entered the cerebrospinal fluid of cattle at a dose of 0.1 ml per 1 kg of live weight, ie 9 ml per head.

Animals of group 2, onocr the IDT, subcutaneously in the field of biologically active points, which is responsible for blood was injected irradiated with ultraviolet rays for 15 minutes cerebrospinal fluid of cattle at a dose of 0.1 ml per 1 kg of live weight, ie 9 ml per head.

During the month of observed clinical condition of experimental animals and were selected for the study blood and red bone marrow at 3, 10 and 30 days after staging experience.

Data analysis and visual assessment of the effectiveness of the cerebrospinal fluid and cerebrospinal fluid irradiated with ultraviolet rays in the field of biologically active points, responsible for the blood to stimulate erythropoiesis presented in figure 1.

From the presented example shows that the cerebrospinal fluid of cattle entered in the field of biologically active points responsible for hematopoiesis, erythropoiesis stimulating. Irradiation cerebrospinal fluid of cattle ultraviolet rays increases its pharmacological activity, allowing you to achieve greater stimulation of erythropoiesis in animals with nutritional anemia, anemic States, and correction of anemia syndromes. From the presented data in animals of both groups were observed stimulation of erythropoiesis, but the animals of the second group it was bol the e pronounced.

Example 2. Research on the effectiveness of the proposed method of promoting blood of animals in comparison with the classical method of treating animals with nutritional anemia prophylaxis anemic States and correction anemic syndromes.

Studies were conducted on the girls black and white breed, age 2 months, live weight of 90 kg, with a diagnosis of anemia, which formed the 2 experimental groups of 20 animals each.

Conditions of feeding and maintenance of animals in both groups was the same. Animals of each group were placed in separate cells.

Animals of the experimental groups before treatment were selected blood and bone marrow to confirm the diagnosis.

The first group was treated according to the usual method of treatment of anemia. For stimulation of hematopoiesis used a complex iron-containing drug - verkopen that saharat contains iron, cobalt gluconate solution and carbohydrates and is a clear liquid with a reddish color. The presence in the preparation of cobalt enhances erythropoiesis. The dose for intravenous calves 3 ml per head. After 10 days, repeated injection. Of vitamin preparations as a stimulator of hematopoiesis, providing blood gets well gemoglobinogennyh erythrocytes, used daily intravenous administration of 40% Rast is ora glucose with addition of 1% solution of ascorbic acid at a dose of 36 ml of working solution on the head.

The second group of animals were treated according to the above method of treatment of anemia and advanced in the area of biologically active points responsible for hematopoiesis (shoulder area on the left side), subcutaneously, once introduced cerebrospinal fluid of cattle, irradiated with ultraviolet rays for 15 minutes at a dose of 0.1 ml per 1 kg of live weight, ie 9 ml per head.

During the month, followed by the clinical condition of experimental animals and were selected for the study blood and red bone marrow for 5, 10, 15, 20, 25 and 30 days after initiated treatment measures.

Data analysis and visual assessment of the efficacy of drugs and irradiated with ultraviolet rays cerebrospinal fluid of cattle entered in the field of biologically active points, which is responsible for blood formation and stimulation of erythropoiesis, is presented in figure 2.

Compared with the known solution, the proposed method can improve the stimulation of erythropoiesis cattle through effective erythropoetic properties of irradiated ultraviolet rays cerebrospinal fluid of cattle. In addition, the costs for their treatment due to the absence of expensive drugs.

Method of stimulating erythropoiesis in cattle, characterized by NR is trimesechie introduction to the shoulder area on the left side irradiated with ultraviolet rays for 15 min cerebrospinal fluid of a single dose of 0.1 ml per 1 kg of live weight.



 

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