Method of dietary supplement production

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention belongs to food, chemical and pharmaceutical, medical, liquor and biotechnological industries. Previously washed in water and homogenously blended fruits of walnuts with pericarps are subjected to extraction. The prepared raw material is steeped in 35-45% water solution of ethyl alcohol in 1:1 weight ratio. The mixture is infused for 6-7 days away from light, then extracted with ultrasound in cavitation mode for 30-60 min at a temperature of 15-18°C; after that extraction is repeated at least 5-6 times, each subsequent extaction being carried out after the mixture has been infused for 3-4 days max. Eventually, the extract is separated by filtration and cooled.

EFFECT: increased content of biologically active substances in a supplement.

3 cl

 

The invention relates to the processing of vegetable raw materials, in particular of green fruit of the walnut, for the production of biologically active additives (BAA) from the extract of green fruits walnuts, and can be used in food, chemical and pharmaceutical, medical, alcohol and biotechnological industries for regulating the functions of the human body in physiological boundaries.

The balance between incoming energy from food and energy costs of human body is the basis of functional foods. This is expressed in mandatory intake of major nutrients (proteins, fats and carbohydrates) in a physiologically required ratios, allowing you to meet the person's need for essential components of food. The most that is both affordable and effective way to optimize power is the widespread use of BAA, which can be defined as concentrates, natural or identical to natural biologically active substances intended for direct reception and / or introduction into food products.

In recent years, owing to adverse environmental conditions, increased attention is paid to methods for production and application of extracts of plant materials, including on the basis of immature Grec is their nuts milky-wax ripeness. Nuts contain biologically active substances, including pigments - carotenoids, catechins, leucoanthocyanin etc. (see Trojan Z.A. and other walnut in milky-wax ripeness - a valuable raw material. // Progressive environmentally safe storage technologies and complex processing of agricultural products for producing food of high nutritional and biological value." Abstracts of scientific conference. 9-10 September 1999, the Russian Academy of agricultural Sciences. M., 1999, - c.406).

Walnut is a deciduous tree of the walnut family. Green fruits are found complex vitamins, carotene, quinones, which have bactericidal, anti-inflammatory, phytoncide, antiparasitic and gormonoreguliruyuschie properties. Antihelminthic action okoloplodnika and green fruit nut known since the time of Hippocrates (see, for example, Pastushenkov " L.V. et al. The medicinal plants. - L., 1990).

Walnut and products based on it are widely used in folk and scientific medicine. Medicinal raw materials are mainly leaves, fresh unripe fruit and pericarp. Immature (green) fruit walnuts contain significant amounts of naphthoquinone uglon with pronounced antimicrobial properties (see Assenov I., Nikolov S. Pharmacognosy. - Sofia: Medicine, FIZKUL is ur 1988, - 466 C.). Walnuts milky-wax ripeness processed into preserves, jams, drinks that have curative properties for a number of diseases.

Know the use of an extract of green walnut and its pericarp during milky-wax ripeness in folk and scientific medicine. The extract of green walnut is not a drug, but its effectiveness is undeniable.

Green walnuts contain fat-soluble vitamins a, E, K, vitamin B1In2, P, PP, carotene and quinones, unsaturated fatty acids - linoleic, linolenic, oleic, palmitic and other organic acids, quercetin, Ugandan, uglon, iodine, zinc, cobalt, potassium, magnesium, iron and other biologically active substances. The shell contains vinylcarbazole acid, tannins and coumarins; pellicula (the skin that covers the fetus) - steroids, tannins. All these ingredients jointly determine the following properties of an extract of green walnut: antibacterial, tonic, anti-sclerotic, astringent, hemostatic, anti-inflammatory, wound healing, necrotic.

From green walnuts in kerosene extracted fatty oil, which is composed of fatty acids - linolenic 3,2-11.4%, and PA is miliolina of 0.3%, linoleic 5.5 to 6.2%, oleic 13-28%, palmitic 5-12%, stearic 1-3%, lauric, myristic, arachnid, quinone, glycoside α-hereupon, tannins 3-4%, vitamin C 0,9-1,5%, alkaloids, plant phenols, chlorophyll, essential oils, fat-soluble vitamins a, E, K, vitamin B1In2, P, PP, organic acids, quercetin, Ugandan, uglon, iodine, zinc, cobalt, potassium, magnesium, iron and other biologically active substances.

A method of obtaining tinctures walnuts milky-wax ripeness: 30 g chopped unripe fruits pour 1 liter of alcohol, insist in the bottle in the sun for 14 days. On the basis of the General conditions of use in tincture, you can add honey (see the Secrets of medicinal herbs. Popular encyclopedia, Minsk, 1995, vol. 1, s, Vol.2, p.7).

The disadvantage of this method is that the resulting tincture is characterized by a low content of biologically active substances, narrow range of pharmacological actions (used to treat anemia, diarrhea, atherosclerosis of the brain, rickets, bleeding and as vermifuge).

A method of obtaining funds for cleaning the liver, intestines, and for the treatment of atherosclerosis: green walnuts pour liquid honey, add vodka and infused for 14 days (see Semenov A.A. Medical settings: helps when alcohol is ol. - SPb.: Nevsky Prospekt, 1999, p.30).

The disadvantage of this method is that obtained by this method, the tool is characterized by a low content of biologically active substances, allergic reactions with a long reception, a narrow range of pharmacological actions.

A method of obtaining extracts (tinctures) immature walnuts in water-alcohol (70% ethanol) solution at the insistence of 10-30 days (see Popular encyclopedia. The secrets of medicinal herbs. Minsk, 1995, T.1, s). Alternatively extraction spend vodka (40% ethanol, water) at a ratio of 1:10 for 2 weeks. Thus recommend the use of extract 1 teaspoon 2 times a day for prevention and treatment of cardiovascular diseases, diseases of the thyroid gland.

The disadvantage of this method is that obtained by this method, the tool is characterized by a low content of biologically active substances.

A method of obtaining a composition based on extracts of walnuts milky-wax ripeness, including chopped nuts, extraction spirit aqueous solution in a ratio of 0.9 to 1.0:10 within 14 days, the separation of the extract, in addition prepare spirit aqueous extract solution of crushed hawthorn fruit in the ratio of 10:0,9-1,0, mix the first and second e is stricty and add to the mixture glycine in the following ratio of components (parts by weight): extract of walnuts 10; extract hawthorn fruit 0,9-1,0; glycine 0,09-0,1 (see RF patent for the invention №2258438, IPC A23L 1/30, AK 35/78, publ. 20.08.2005 year).

A disadvantage of the known method of obtaining a composition based on extracts of walnuts milky-wax ripeness is that when a fairly complex technology product obtained is insufficient contains biologically valuable components.

A method of obtaining the extract on the basis of walnuts, including chopped nuts milky-wax ripeness with allopatrically, drying to air-dry state, mixing with powdered additive in air-dry condition, the processing of aviation kerosene with stirring at room temperature, and the drying of nuts carried out at a temperature of 60-65°C, nuts mixed with shredded to a size of 5-10 mm, the hips, the mass ratio of 100:3-8, pour extractant - aviation kerosene at a ratio of raw materials-extractant 1:2-3 and the extraction is carried out within 14-20 days (see RF patent for invention No. 2244549, IPC AC 35/00, AK 35/78, publ. 20.01.2005,).

However, the extract produced by a known method, insufficient contains biologically valuable components. In addition, at high temperatures decompose a complex of biologically active components of natural raw materials and continuous mixing requires special is the material of the equipment and increases the cost of the drug.

A method of obtaining extracts (tinctures) of walnuts when using as extractant 70%ethyl alcohol in water at the insistence of 10-30 days, or 40%spirit aqueous solution at a mass ratio of raw material:solvent 1:10 - within 2 weeks (see, for example, A.A. Semenova //Medicinal tinctures: when alcohol helps. St. Petersburg, publishing house "Nevsky Prospekt", 1999, p.27-30). The extract was applied, for example, to receive one teaspoon 2 times a day for prevention and treatment of cardiovascular diseases and diseases of the thyroid gland.

However, the extract produced in a known manner, has insufficient content of biologically active substances, as well as a limited shelf life.

Also known is a method of obtaining the extract biologically active pigments on the basis of walnuts, including grinding, mixing with spirit aqueous solution, the infusion for 14 days and the Department in the form of a liquid phase, while the extraction is carried out spirit aqueous solution of the dried chopped walnuts milky-wax ripeness in the ratio spirit aqueous solution:a nuts 10:0,9-1,0 and insist at room temperature (see RF patent for the invention №2253470, IPC AC 35/78, publ. 10.06.2005,). The method is as follows. Walnuts milky-wax SP is lost first crushed to particles-slices the thickness of 6-8 mm, dried at a temperature of 60-65°C until constant weight, ground to particles of not more than 3 mm and a residual moisture content of not more than 10%, is mixed with spirit aqueous solution in a ratio of 0.9 to 1.0:10, 14 days, separating the extract and Packed in a sealed container; keep at temperature not exceeding 30°C in a dark place for 6 months without changing the quality.

The disadvantage of this method is that when a fairly complex technology product obtained is not a high biological value.

A method of obtaining preventive restorative compositions on the basis of nuts milky-wax ripeness and honey, including chopped nuts and seasoning them with honey and water-alcohol solution, while insisting nuts in honey and water-alcohol solution are separately and additionally introducing water-alcohol extract of flower pollen, 10%aqueous solution of glycine and alcohol in the following ratio, wt.%: water-alcohol infusion nuts - 45,00; aqueous-alcoholic extract of flower pollen - 0,03; an aqueous solution of glycine - 0,04; honey infusion of nut - 14,00; alcohol - rest, and alcohol injected at a concentration providing a 40%reduction in the fortress of the entire composition. Water-alcohol infusion walnuts cooked in a ratio of nuts:60% alcohol = 1:3, and insist 20 days. The extract of the flower the pollen is prepared in the ratio of pollen:40% alcohol = 1:10, survive 5 days, then filtered. Honey infusion walnuts cooked in a ratio of nuts:honey = 1:5 and insist in a dark place within 42 days. The composition is prepared by sequentially introducing components in the following order: water-alcohol infusion walnuts, water-alcohol extract of flower pollen, an aqueous solution of glycine, honey infusion walnuts, mix thoroughly, bring to 40% of the fortress and incubated for 2 h (see RF patent for the invention №2223112, IPC AC 35/78, OR 3/00, AR 37/04, AR 5/14, AR 31/12, AR 31/10, OR 33/10, publ. 10.02.2004,).

The disadvantage of this method of obtaining preventive restorative compositions is that when a fairly complex technology product obtained is insufficient contains biologically valuable components.

Also known is a method of obtaining an extract of walnuts milky-wax ripeness, including the grinding of raw materials, mixing with the extractant in a mass ratio of 1:1 and extraction at room temperature, the extractant used medical Dimexidum, raw material is crushed to a size of 5-7 mm, and the extraction was carried out for 3 months at a temperature of 25-35°C and periodic stirring, 1 time per day (see RF patent for the invention №2239649, IPC SV 1/10, publ. 10.11.2004,).

The disadvantages of the extract obtained by the known is th way are as follows: the extract is used for external use only; within 90 days every day needs mixing, which increases labor costs; the extraction is carried out with the access of light and air, which leads to additional oxidation of oxygen and the expression of the light and reduces the biological value of the product.

A method of obtaining an extract of plant materials on the basis of walnuts, including grinding plant, mixing with aviation kerosene, extraction, while the plant material further comprises dry birch mushroom, chaga at the following ratio: 10 parts by weight of walnut raw materials have to 0.1 parts by weight of pollen, from 0.2 to 0.22 parts by weight of propolis and 0.5-0,54 parts by weight of birch mushroom, chaga (see RF patent for the invention №2228189, IPC AC 35/78, publ. 10.05.2004,).

The disadvantage is that the extract contains plant material, which includes pollen, and extraction of aviation kerosene dry plant material, while protein particles, giving the water will not dissolve in kerosene and in pure alcohol, which leads to insufficient content in the extract biologically valuable components.

It is also known that the processes of extraction greatly accelerated under the action of ultrasound. The process extracts and is not only much faster but at much lower temperatures, which is important for substances that decompose or oxidize at high temperatures. The physical basis of this acceleration lies in the destruction of the boundary layer, resulting in faster mass transfer between the surface of the raw material and the extractant (see, for example, Physical encyclopedic dictionary. M: Soviet encyclopedia, 1966, - V.5, - s-237).

Closest to the proposed technical solution is known a method of obtaining biologically active drug by extraction of immature fruits of walnut with an organic solvent, with walnuts milky-wax ripeness in the green pericarp crushed to particle size of 1 mm, extracted fraction from crude oil with a low content of hydrocarbons when the ratio of raw material and extractant 1:3 and vigorous stirring, then the mixture was kept and the extract is separated, the remaining suspension is again treated with the same extractant under vigorous stirring and the ratio of raw material and extractant 3:1, the mixture is then incubated, the extract is separated and both extract combine remaining the pulp is dried and passed through it to extract. Intensive mixing in the first stage extraction can be performed during the first 7 hours of extraction, and the second 14 days. After an intense AC is shivani mixture in the first stage extraction survive 5 days and in the second stage, 10 days. When this extraction on the first and second stages are carried out under vigorous stirring, resulting in increased temperature of the mixture up to 35-40°C and extraction is carried out at elevated temperature (see RF patent for the invention №2033803, IPC AC 35/78, publ. 30.04.1995 year).

However, the product obtained by a known method, insufficient contains biologically valuable components as the extraction of the first and second stages is carried out at elevated temperature, which does not allow to preserve the biological activity of the protein compounds of the feedstock.

The present invention is to preserve the biological activity of used vegetable raw materials.

The technical result achieved in the solution of this problem is lowering the temperature of the processing of ingredients of biologically active additives, which retains the properties of the ingredients and accordingly increases the quality of the dietary Supplement.

This technical result is achieved due to the fact that the method of obtaining biologically active additives by extraction from green walnut with pericarp according to the invention for the extraction using pre-washed with water unripe fruits green walnut with pericarp, which ISM is lcout into a homogeneous mass, the obtained raw material is poured 35-45%aqueous solution of ethyl alcohol at a mass ratio of ingredients is a 1:1 mixture infused for 6-7 days without access of light extracted using ultrasonic cavitation mode for 30-60 minutes at a temperature of 15-18°C, while the extraction was repeated at least 5-6 times, and each subsequent extraction is performed after infusion of the mixture for a period of not more than 3-4 days at a temperature of 10°C, then the extract is separated by filtration and cool.

Preferably, the green walnut pericarp was crushed into powder with a particle size of from 10 to 20 mm.

Appropriate cooling of the extract after filtration to carry out up to a temperature of not more than 10°C.

Use as extractant aqueous solution of ethyl alcohol is the traditional and most "loyal" when retrieving complex of biologically active substances from plant materials.

The concentration of the extractant (35-45%aqueous ethanol solution) was determined experimentally and provides a complete and comprehensive extraction of biologically active compounds of different nature, providing the necessary level and range of the specific activity of the target product, its quality. Thus, when the concentration of the extractant less than the claimed value (35%) was observed imbalance is mobilnyh substances and flavonoids: retrieves the maximum tannins and a minimum of flavonoids. When the concentration of the extractant more proposed values (45%) retrieves the minimum tannins and a maximum of flavonoids. All this, ultimately, leads to lower levels and narrowing the spectrum of the specific activity of the target product, the deterioration of its quality.

The number of alcohol for extraction was determined experimentally: the minimum concentration of an alcohol extract of the unripe fruit is green walnut with pericarp is achieved when the mass ratio of ingredients is 1:1, as at lower concentrations is too small a number of biologically active substances in the extract at higher concentration of biologically active substances harder separated from the plant material. Within the specified concentration provided the greatest yield of the final product at the lowest duration of the extraction process. In addition, when alcohol concentration is less than 35% of the extraction process is unacceptable longer, and when alcohol concentration is greater than 45% decreases the yield of biologically active substances.

Preferably, the seasoning mixture before the first extraction for at least 6 days at room temperature not exceeding 20°C, i.e. within the time required for diffusion of the solvent (35-45%aqueous ethanol solution) into powdered product grow from inogo raw (unripe fruit is green walnut with pericarp), when this occurs, the settling of larger particles and the stratification of the mixture for more efficient extraction.

As a result of processing the mixture by ultrasonic cavitation mode due to the intensive interaction of the extractant with extractant with high frequency ultrasonic impact and cavitation mechanism of interaction is efficient extraction process. Such treatment allows the maximum output in the extract intracellular fluid containing biologically active substances. While the extraction process is not only much faster, but at much lower temperatures, which is important for substances that decompose or oxidize at high temperatures, in particular for the preservation of the biological activity of the protein compounds of the feedstock.

Carrying out the extraction of raw materials, filled 35-45%aqueous solution of ethyl alcohol, by means of ultrasound at least 5-6 times is necessary and sufficient to achieve complete extraction of biologically active compounds, the complex which provides the necessary level and range of the specific activity of the target product. With fewer extraction is not complete depletion of raw materials. The implementation of more extraction in the present method, in contrast to p is ototype, it is impractical and leads to excess extractant.

The seasoning mixture before each re-extraction for a period of not more than 3-4 days at a temperature of 10°C is a necessary and sufficient condition for precipitation of high molecular weight proteins, the presence of which in the target product is undesirable, as it leads to deterioration of its quality and the appearance of toxic effects in its application. The use of other methods of extraction of high-molecular substances, such as ultrafiltration, on the one hand, it is impractical, as it adds cost and complexity to the conduct of the process. On the other hand, may lead to violation of the structural-mechanical characteristics of other biologically active compounds.

The process of infusion required to separate the solids and obtain the target product, the relevant pharmacopoeial requirements.

The pericarp enriched extract of organic acids (citric, malic), vitamin C, besides tannins in it 8.5 times more than in the nuts (35%). The completeness of the extraction depends on the difference in concentrations of extractable substances in the liquid and solid phase. Therefore, re-extraction provides more complete recovery of the total amount of biologically active substances from walnut on the yekha.

The timing extraction using ultrasound is determined empirically depending on the desired concentration of the resulting alcohol extract. The experiment showed that the extraction is advisable to carry out a period of not more than 60 min, and then further to repeat them at least 5-6 times with an interval between extraction no more than 3-4 days, during which there is a seasoning mixture and dorstenia particle mixtures that have not been extracted. This leads to a qualitative extraction due to more complete dissolution nephroptosis particle mixture. The choice of temperature of extraction does not exceed 18°C due to the persistence of biological activity of the protein compounds of the feedstock and obtaining alcoholic extract with a high concentration of the final product, as opposed to insisting upon the proposed method of obtaining alcoholic extract biologically active substances are released from vegetable raw materials in larger quantities and in malaysiana. In the extraction process control temperature of the extract using a known measurement tools.

Then the extract is separated by filtration and cooled to the storage temperature is not more than 10°C, while the quality of the final product of the extract in the alcohol tincture with more permanent the major period of storage.

The method of obtaining biologically active additives by extraction of green fruits walnuts pericarp is as follows.

To obtain plant materials used for the manufacture of dietary SUPPLEMENTS, green walnuts in the period milky-wax ripeness (21-23 day after flowering) together with the pericarp is collected from wild trees, pre-washed with water (for example, carry out the removal of dust, dirt, sand, stones, straw, metal, other impurities, shell, and microorganisms) and ground in a small fraction in the powder particles is from 10 to 20 mm Grinding homogeneous mass of plant materials to a powder, carried out on the well-known mills, for example, a hammer micromelia type M-8M (factory LLC "Platejnoe", Ryazan region, Rybnoe), or other known shredders closed type.

The resulting mass of plant materials (with a shelf life up to 1 year) is used to produce alcoholic extract of green fruits walnuts. To this end, the raw materials (mass) is placed in a vessel and pour 35-45%aqueous solution of ethyl alcohol at a mass ratio of ingredients is 1:1. Chopped walnuts should be covered by 1.0-15 cm layer of alcohol. The vessel is placed in a location with temperatures between 10°C, where no access is as light, and insist for 6-7 days. The mixture is then placed in an ultrasonic reactor for active extraction of biologically active substances in a solvent, the sonication is carried out in the cavitation mode for 30-60 minutes at a temperature of 15-18°C.

As ultrasonic reactor can be used, for example, the installation of UZUMI-15" (manufactured by LLC "TRIM", Saratov), power consumption 750 V•a, the frequency of ultrasonic vibrations 38 kHz ultrasonic power 550 V•a (see, for example, an Internet site, the access mode: http://www.trima.ru/ autoservice/uzumi_ind.htm). The qualitative effect of the active extraction is achieved due to the formation of liquid ultrasonic cavitation - pressure pulses arising from the closure of cavitation voids, the impact of mikropotokami and micromachine the cavitating solution in an ultrasonic field on the surface of the particles of the powdered plant materials, including fruit of the walnut in the stage of milky-wax ripeness with allopatrically,

During ultrasonic (active) extraction, the following occurs. Small particles of green walnut and pericarp soaked in 40%ethanol, subjected to the active influence of ultrasound. Resulting in a large amount of dissolved biologically active ve the EU ETS, contained in the nut. Given the low temperature extraction (no more than 15-18°C) content (40%alcohol and chopped green walnuts along with pericarp) is placed again in a dark cool place.

The entire course of extraction lasts from 15 to 24 days with periodic sonication (1 every 3-4 days). I.e. the extraction was repeated at least 5-6 times, with each subsequent extraction is performed after infusion of the mixture. Then the alcohol extract is separated by filtration through gauze three-layer filter, made, for example, from medical gauze bandage sterile (GOST 1172-93 "medical gauze Bandages. Technical conditions"), cooled and poured into glass containers from 10 to 100 ml with subsequent packing of the finished product. BAD Packed in glass or plastic containers, stored in dry conditions, the warranty period is 1 year.

In the production of the BAD uses raw materials that meet the requirements of the relevant standards: rectified ethyl alcohol according to GOST R 51652-2000 "rectified ethyl Alcohol from food raw material. Technical conditions", drinking water according to GOST R 51232-98 drinking Water. General requirements for the organization and methods of quality control".

The proposed method of obtaining BAD by extraction from plant material, which is used as the green circle walnut in the period milky-wax ripeness with allopatrically, illustrated by the following example.

Example. To get BAD on the proposed method plant material, including fruit of the walnut in the stage of milky-wax ripeness with allopatrically, were collected from wild trees and pre-cleaned. When assembled green walnut filled with clean water, as a result, the dirt is dissolved, then raw 30-40 minutes was filtered stream of clean water. If the nut flooded more than 40-60 minutes, then starts the extraction BAS nut into the water.

Then the raw materials were ground to fine fraction in the mass of the particles is not more than 20 mm Grinding plant to powder was carried out at hammer micromelia type M-8M. To obtain a liquid extract of the raw material was filled in a 40%aqueous solution of ethanol at the rate of 100 grams of powder from plant material in 100 ml of 40%aqueous solution of ethyl alcohol. The resulting mixture was poured in a container, tightly closed, and insist in a dark place for 6 days at a temperature not exceeding 20°C. Then the mixture was placed at 45 min in an ultrasonic installation "UZUMI-15". Treatment of the mixture was carried out by ultrasound in the cavitation mode at a temperature of 18°C and was repeated five times with an interval between extraction in 3 days. Then the mixture was filtered through mA who left three-layer filter gauze bandage sterile (GOST 1172-93). The resulting alcoholic extract was decanted into glass containers from 10 to 100 ml, packaged in a container and stored prior to use without access of light. In this extract long retained its activity, including due to the preservative properties of alcohol.

The proposed technology allows to extract from plant material maximum for a given solvent, the amount of biologically active substances, vitamins (C, E, PP, B1In2In6, carotenoids, flavonoids, organic acids, sterols, plant phenols, naftochinona, micro - and macro-elements). Important extractable substance from the nut of the raw material is iodine, so the drug is a reliable tool for the prevention and treatment of diseases related to iodine deficiency in the body.

Manufactured under the proposed method SUPPLEMENTS can be used as a tonic, positively affecting the immune system. The extract obtained as rich in biologically active substances, in short (short) time compared with traditional extraction process, which lasts for 90 days without any processing, including ultrasound.

Thus, for the production of NUTRACEUTICALS from plant material, which is used as green walnuts in the period milky-wax ripeness with what maloplodnoy on the proposed method does not require the development of special equipment, and the cheapness of the raw materials used ensures low cost of production, which can be used as a tonic with antioxidant and adaptogenic properties that help to improve the health and sustainability of the human organism to adverse environmental factors. The extract obtained is suitable for a wide range of consumers, has a strong immunoprotective properties, promotes healing, non-toxic, possesses neither autologous nor immunotoxic, no teratogenic activity.

Biologically active additive is an alcohol tincture dark brown, provides nutritious and of high biological value, safety for human health and the environment; used as an additive in food products.

1. The method of obtaining biologically active additives by extraction of green walnut with pericarp, characterized in that for the extraction using pre-washed with water unripe fruits green walnut with pericarp, which are crushed into a homogeneous mass, the obtained raw material is poured 35-45%aqueous solution of ethyl alcohol at a mass ratio of ingredients is a 1:1 mixture infused for 6-7 days without access to the Board, extracted using ultrasonic cavitation mode for 30-60 minutes at a temperature of 15-18°C, while the extraction was repeated at least 5-6 times, and each subsequent extraction is performed after infusion of the mixture for a period of not more than 3-4 days, then the extract is separated by filtration and cool.

2. The method according to claim 1, characterized in that the green walnut pericarp crushed into powder with a particle size of from 10 to 20 mm.

3. The method according to claim 1, characterized in that the cooling of the extract is carried out to a temperature of not more than 10°C.



 

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2 cl, 3 tbl, 20 ex

FIELD: chemistry.

SUBSTANCE: invention relates to the chemical and pharmaceutical industry. Schizandra seeds are extracted using 70-90% ethyl alcohol first at room temperature and then while heating at temperature between 65-70°C.

EFFECT: invention increases output of active substances.

2 ex, 1 tbl, 1 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention is a pharmaceutical composition for psoriasis and eczema treatment, containing trailing mahonia extract in loposomal delivery system. The extract content in the composition is 5-20% of the composition total weight. The invention also concerns method for production of trailing mahonia extract.

EFFECT: pharmaceutical composition is especially effective for psoriasis and eczema treatment among patients of various degrees of disease.

8 cl

FIELD: medicine, veterinary science.

SUBSTANCE: invention concerns veterinary medicine. Treatment-and-preventive agent for young farm animals is alcoholic tincture of vegetable raw material, the raw material being a mixture of grass and blossom cluster of purple Echinacea, coltsfoot, Syrian rue grass and licorice roots. The vegetable raw material is steeped in 70% ethyl alcohol in proportion 1:10, then kept in dark room at a temperature of 15-20°C for 7 days, strained. The raw material is press out and the tincture is filtered. Method for preventing respiratory diseases includes passive immunisation performed through hypodermic injection of hyperimmune serum containing specific antibodies to rednose, bird flue 3 and bovine viral diarrhea viruses in titres of at least 1:1280, 1:256 and 1:1024 respectively, in dose of 1.5 - 2.0 ml/kg of animal body weight for 15 days with an interval of 12 - 24 hours.

EFFECT: improved efficiency of the agent and prevention method.

2 cl, 2 tbl, 2 ex

FIELD: medicine, veterinary science.

SUBSTANCE: invention concerns veterinary medicine. Treatment-and-preventive agent for young farm animals is alcoholic tincture of vegetable raw material, the raw material being a mixture of grass and blossom cluster of purple Echinacea, coltsfoot, Syrian rue grass and licorice roots. The vegetable raw material is steeped in 70% ethyl alcohol in proportion 1:10, then kept in dark room at a temperature of 15-20°C for 7 days, strained. The raw material is press out and the tincture is filtered. Method for preventing respiratory diseases includes passive immunisation performed through hypodermic injection of hyperimmune serum containing specific antibodies to rednose, bird flue 3 and bovine viral diarrhea viruses in titres of at least 1:1280, 1:256 and 1:1024 respectively, in dose of 1.5 - 2.0 ml/kg of animal body weight for 15 days with an interval of 12 - 24 hours.

EFFECT: improved efficiency of the agent and prevention method.

2 cl, 2 tbl, 2 ex

FIELD: medicine, veterinary science.

SUBSTANCE: invention concerns veterinary medicine. Treatment-and-preventive agent for young farm animals is alcoholic tincture of vegetable raw material, the raw material being a mixture of grass and blossom cluster of purple Echinacea, coltsfoot, Syrian rue grass and licorice roots. The vegetable raw material is steeped in 70% ethyl alcohol in proportion 1:10, then kept in dark room at a temperature of 15-20°C for 7 days, strained. The raw material is press out and the tincture is filtered. Method for preventing respiratory diseases includes passive immunisation performed through hypodermic injection of hyperimmune serum containing specific antibodies to rednose, bird flue 3 and bovine viral diarrhea viruses in titres of at least 1:1280, 1:256 and 1:1024 respectively, in dose of 1.5 - 2.0 ml/kg of animal body weight for 15 days with an interval of 12 - 24 hours.

EFFECT: improved efficiency of the agent and prevention method.

2 cl, 2 tbl, 2 ex

FIELD: medicine, veterinary science.

SUBSTANCE: invention concerns veterinary medicine, namely to agents prepared from medical plants. The invention consists in the following. Mixture of the following medical plants crushed to fine and ultrafine particles and treated with bactericidal lamps rays are placed in a sterile tank of dark glass: dry stalks, leaves and flowers of peppermint, dry flowers of wild chamomile, dry leaves of waybread and dry flower heads of costmary (Tanacetum vulgare), dry stalks, leaves and flowers of yellow melilot, dry grass of St. John's wort, dry flowers of pot marigold, dy grass of milfoil, dy grass of thyme, dry stalks, leaves, flowers and fruits of celandine, dry leaves of eucalyptus, dry leaves and flower heads of coltsfoot, dry leaves of garden sage, dry leaves of stinging nettle, dry grass of common persicaria, dry flowers of densely flowered mullein, bark of ordinary oak, roots of common licoric, ground fruits of anise and rose hips, walnut kernel, buckthorn pulp without kernels, their fruits and berries. These components are treated with bactericidal lamps rays and crushed to fine and ultrafine particles, then placed to a tank of dark glass, mixed with sterile cooking salt solution with 0.9% sodium content, and with formalin. The mixture is left and room temperature for 3-4 days, regularly stirred, and then placed to water bath for 10-12 hours and exposed to temperature up to 40°, with contiuous stirring. After that the liquid part is separated from the vegetable sediment. The tincture is stored in tanks of dark glass or in a darkened room at a temperature of 2-4°C, and is applied externally and internally, and the vegetable sediment is used as fodder and remedy for animals and birds, especially for chickens in case of cannibalism.

EFFECT: at experimental checks of therapeutic efficacy of the agent, good results were achieved in prevention and treatment of inflammatory processes among animals; wide application in veterinary.

3 cl

FIELD: medicine, veterinary science.

SUBSTANCE: invention concerns veterinary medicine, namely to agents prepared from medical plants. The invention consists in the following. Mixture of the following medical plants crushed to fine and ultrafine particles and treated with bactericidal lamps rays are placed in a sterile tank of dark glass: dry stalks, leaves and flowers of peppermint, dry flowers of wild chamomile, dry leaves of waybread and dry flower heads of costmary (Tanacetum vulgare), dry stalks, leaves and flowers of yellow melilot, dry grass of St. John's wort, dry flowers of pot marigold, dy grass of milfoil, dy grass of thyme, dry stalks, leaves, flowers and fruits of celandine, dry leaves of eucalyptus, dry leaves and flower heads of coltsfoot, dry leaves of garden sage, dry leaves of stinging nettle, dry grass of common persicaria, dry flowers of densely flowered mullein, bark of ordinary oak, roots of common licoric, ground fruits of anise and rose hips, walnut kernel, buckthorn pulp without kernels, their fruits and berries. These components are treated with bactericidal lamps rays and crushed to fine and ultrafine particles, then placed to a tank of dark glass, mixed with sterile cooking salt solution with 0.9% sodium content, and with formalin. The mixture is left and room temperature for 3-4 days, regularly stirred, and then placed to water bath for 10-12 hours and exposed to temperature up to 40°, with contiuous stirring. After that the liquid part is separated from the vegetable sediment. The tincture is stored in tanks of dark glass or in a darkened room at a temperature of 2-4°C, and is applied externally and internally, and the vegetable sediment is used as fodder and remedy for animals and birds, especially for chickens in case of cannibalism.

EFFECT: at experimental checks of therapeutic efficacy of the agent, good results were achieved in prevention and treatment of inflammatory processes among animals; wide application in veterinary.

3 cl

FIELD: medicine, veterinary science.

SUBSTANCE: invention concerns veterinary medicine, namely to agents prepared from medical plants. The invention consists in the following. Mixture of the following medical plants crushed to fine and ultrafine particles and treated with bactericidal lamps rays are placed in a sterile tank of dark glass: dry stalks, leaves and flowers of peppermint, dry flowers of wild chamomile, dry leaves of waybread and dry flower heads of costmary (Tanacetum vulgare), dry stalks, leaves and flowers of yellow melilot, dry grass of St. John's wort, dry flowers of pot marigold, dy grass of milfoil, dy grass of thyme, dry stalks, leaves, flowers and fruits of celandine, dry leaves of eucalyptus, dry leaves and flower heads of coltsfoot, dry leaves of garden sage, dry leaves of stinging nettle, dry grass of common persicaria, dry flowers of densely flowered mullein, bark of ordinary oak, roots of common licoric, ground fruits of anise and rose hips, walnut kernel, buckthorn pulp without kernels, their fruits and berries. These components are treated with bactericidal lamps rays and crushed to fine and ultrafine particles, then placed to a tank of dark glass, mixed with sterile cooking salt solution with 0.9% sodium content, and with formalin. The mixture is left and room temperature for 3-4 days, regularly stirred, and then placed to water bath for 10-12 hours and exposed to temperature up to 40°, with contiuous stirring. After that the liquid part is separated from the vegetable sediment. The tincture is stored in tanks of dark glass or in a darkened room at a temperature of 2-4°C, and is applied externally and internally, and the vegetable sediment is used as fodder and remedy for animals and birds, especially for chickens in case of cannibalism.

EFFECT: at experimental checks of therapeutic efficacy of the agent, good results were achieved in prevention and treatment of inflammatory processes among animals; wide application in veterinary.

3 cl

FIELD: medicine, veterinary science.

SUBSTANCE: invention concerns veterinary medicine, namely to agents prepared from medical plants. The invention consists in the following. Mixture of the following medical plants crushed to fine and ultrafine particles and treated with bactericidal lamps rays are placed in a sterile tank of dark glass: dry stalks, leaves and flowers of peppermint, dry flowers of wild chamomile, dry leaves of waybread and dry flower heads of costmary (Tanacetum vulgare), dry stalks, leaves and flowers of yellow melilot, dry grass of St. John's wort, dry flowers of pot marigold, dy grass of milfoil, dy grass of thyme, dry stalks, leaves, flowers and fruits of celandine, dry leaves of eucalyptus, dry leaves and flower heads of coltsfoot, dry leaves of garden sage, dry leaves of stinging nettle, dry grass of common persicaria, dry flowers of densely flowered mullein, bark of ordinary oak, roots of common licoric, ground fruits of anise and rose hips, walnut kernel, buckthorn pulp without kernels, their fruits and berries. These components are treated with bactericidal lamps rays and crushed to fine and ultrafine particles, then placed to a tank of dark glass, mixed with sterile cooking salt solution with 0.9% sodium content, and with formalin. The mixture is left and room temperature for 3-4 days, regularly stirred, and then placed to water bath for 10-12 hours and exposed to temperature up to 40°, with contiuous stirring. After that the liquid part is separated from the vegetable sediment. The tincture is stored in tanks of dark glass or in a darkened room at a temperature of 2-4°C, and is applied externally and internally, and the vegetable sediment is used as fodder and remedy for animals and birds, especially for chickens in case of cannibalism.

EFFECT: at experimental checks of therapeutic efficacy of the agent, good results were achieved in prevention and treatment of inflammatory processes among animals; wide application in veterinary.

3 cl

FIELD: medicine, veterinary science.

SUBSTANCE: invention concerns veterinary medicine, namely to agents prepared from medical plants. The invention consists in the following. Mixture of the following medical plants crushed to fine and ultrafine particles and treated with bactericidal lamps rays are placed in a sterile tank of dark glass: dry stalks, leaves and flowers of peppermint, dry flowers of wild chamomile, dry leaves of waybread and dry flower heads of costmary (Tanacetum vulgare), dry stalks, leaves and flowers of yellow melilot, dry grass of St. John's wort, dry flowers of pot marigold, dy grass of milfoil, dy grass of thyme, dry stalks, leaves, flowers and fruits of celandine, dry leaves of eucalyptus, dry leaves and flower heads of coltsfoot, dry leaves of garden sage, dry leaves of stinging nettle, dry grass of common persicaria, dry flowers of densely flowered mullein, bark of ordinary oak, roots of common licoric, ground fruits of anise and rose hips, walnut kernel, buckthorn pulp without kernels, their fruits and berries. These components are treated with bactericidal lamps rays and crushed to fine and ultrafine particles, then placed to a tank of dark glass, mixed with sterile cooking salt solution with 0.9% sodium content, and with formalin. The mixture is left and room temperature for 3-4 days, regularly stirred, and then placed to water bath for 10-12 hours and exposed to temperature up to 40°, with contiuous stirring. After that the liquid part is separated from the vegetable sediment. The tincture is stored in tanks of dark glass or in a darkened room at a temperature of 2-4°C, and is applied externally and internally, and the vegetable sediment is used as fodder and remedy for animals and birds, especially for chickens in case of cannibalism.

EFFECT: at experimental checks of therapeutic efficacy of the agent, good results were achieved in prevention and treatment of inflammatory processes among animals; wide application in veterinary.

3 cl

FIELD: medicine, veterinary science.

SUBSTANCE: invention concerns veterinary medicine, namely to agents prepared from medical plants. The invention consists in the following. Mixture of the following medical plants crushed to fine and ultrafine particles and treated with bactericidal lamps rays are placed in a sterile tank of dark glass: dry stalks, leaves and flowers of peppermint, dry flowers of wild chamomile, dry leaves of waybread and dry flower heads of costmary (Tanacetum vulgare), dry stalks, leaves and flowers of yellow melilot, dry grass of St. John's wort, dry flowers of pot marigold, dy grass of milfoil, dy grass of thyme, dry stalks, leaves, flowers and fruits of celandine, dry leaves of eucalyptus, dry leaves and flower heads of coltsfoot, dry leaves of garden sage, dry leaves of stinging nettle, dry grass of common persicaria, dry flowers of densely flowered mullein, bark of ordinary oak, roots of common licoric, ground fruits of anise and rose hips, walnut kernel, buckthorn pulp without kernels, their fruits and berries. These components are treated with bactericidal lamps rays and crushed to fine and ultrafine particles, then placed to a tank of dark glass, mixed with sterile cooking salt solution with 0.9% sodium content, and with formalin. The mixture is left and room temperature for 3-4 days, regularly stirred, and then placed to water bath for 10-12 hours and exposed to temperature up to 40°, with contiuous stirring. After that the liquid part is separated from the vegetable sediment. The tincture is stored in tanks of dark glass or in a darkened room at a temperature of 2-4°C, and is applied externally and internally, and the vegetable sediment is used as fodder and remedy for animals and birds, especially for chickens in case of cannibalism.

EFFECT: at experimental checks of therapeutic efficacy of the agent, good results were achieved in prevention and treatment of inflammatory processes among animals; wide application in veterinary.

3 cl

FIELD: medicine, oncology, amino acids.

SUBSTANCE: invention relates, in particular, to the development of an antitumor preparation based on natural substances. Invention relates to an amino acid preparation comprising at least one modified essential amino acid obtained by treatment of amino acid by ultraviolet radiation (UV) at wavelength 250-350 nm for 12-80 h at temperature 15-30oC or with ozone at temperature 15-25oC. The modified amino acid has no toxicity for health cells. Also, invention relates to a method for preparing such preparation. Invention provides the development of an antitumor preparation based on modified amino acids and expanded assortment of antitumor preparations being without cytotoxicity for normal cells.

EFFECT: valuable medicinal antitumor properties of preparation.

8 cl, 4 tbl, 2 dwg, 4 ex

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