Treatment-and-preventive agent for young farm animals; and method for prevention of respiratory diseases among calve and its application

FIELD: medicine, veterinary science.

SUBSTANCE: invention concerns veterinary medicine. Treatment-and-preventive agent for young farm animals is alcoholic tincture of vegetable raw material, the raw material being a mixture of grass and blossom cluster of purple Echinacea, coltsfoot, Syrian rue grass and licorice roots. The vegetable raw material is steeped in 70% ethyl alcohol in proportion 1:10, then kept in dark room at a temperature of 15-20°C for 7 days, strained. The raw material is press out and the tincture is filtered. Method for preventing respiratory diseases includes passive immunisation performed through hypodermic injection of hyperimmune serum containing specific antibodies to rednose, bird flue 3 and bovine viral diarrhea viruses in titres of at least 1:1280, 1:256 and 1:1024 respectively, in dose of 1.5 - 2.0 ml/kg of animal body weight for 15 days with an interval of 12 - 24 hours.

EFFECT: improved efficiency of the agent and prevention method.

2 cl, 2 tbl, 2 ex

 

The invention relates to veterinary medicine, refers to a therapeutic and preventive tool for young farm animals and can be used for the prevention of respiratory disease in calves.

Mass respiratory disease of young farm animals are complex infectious processes, in which different stages of the pathological process involved bacteria, viruses, mycoplasmas in various combinations. Action mechanism of acute respiratory disease of calves are viruses (mainly viruses parainfluenza-3, infectious bovine rhinotracheitis, virus diarrhea-diseases of the mucous membranes, respiratory syncytial virus), which causes damage to the genetic structure of cells, which lowers resistance to bacterial infections. Mass respiratory diseases occur as mixed viral and viral-bacterial infection, characterized especially malignant, which are difficult to diagnose because of secondary bacterial infection "masks" primary viral disease. Associated viral and bacterial pathogens, as an etiological factor, creates several problems in the treatment of sick animals. Infection with most viruses leads to accumulation of humoral antibodies. However, their level fast what about the falls, which leads to the development of secondary immune response and disease modification.

Known methods of prevention and treatment of respiratory disease in calves, based on antibiotic therapy. The basic principles of antibiotic therapy in respiratory diseases are: the use of high single and daily doses, continuity of administration, duration of administration to the disappearance of the signs of an inflammatory process, using various routes of administration /1/. However, it is established that the use of antibiotics for respiratory pathologies leads to inhibition of the activity of a number of tissue enzymes and factors of humoral and cellular immunity /2/. This disadvantage is eliminated by the inclusion in the scheme of treatment with antibiotics tools to boost natural resistance and immunological protection (vitamins, minerals, non-specific immunoglobulin, serum of convalescents and other) /3/. Alternative antibiotic therapy is herbal medicine. The value of medicinal plants is that they are low-toxic, softly influence an organism, can be used long-term. Thus, the collection of medicinal herbs including nettle, burdock, chamomile pharmacy, oregano ordinary, St. John's wort and common wormwood, improved security of calves when bolesna the respiratory organs in 10-32% /4/, and collection, including St. John's wort, creeping thyme, peppermint, mother-and-stepmother - activation of the immune system, particularly T - phagocytic, which aggravate the pathological process and the effectiveness of treatment of respiratory diseases /5/.

The basis of reduced body resistance animals are immunodeficiencies, against which animals are infected with bacterial and viral pathogens and inflammatory processes often proceed with irreversible morphological abnormalities in cells of organs and tissues.

The immune status of calves with respiratory disease increase passive immunization using immune sera of animal blood containing antibodies to pathogens that form the viral-bacterial background livestock complex or immune serum donor animals, such as hyperimmune serum containing hemagglutinin to viruses RTI, PG-3 and BM-BS /6/.

The technical result is to create a new simple in manufacture of the medicinal product with an extended spectrum of activity and high effect for respiratory diseases of calves against the background of passive immunization.

Getting the specified result is achieved by the use of therapeutic and prophylactic substances, representing an alcoholic tincture of ground flora is OI a mixture of herbs and blossoms of purple coneflower, mother-and-stepmother, grass Rue ordinary and roots of licorice, taken in equal amounts.

Used ingredients therapeutic and preventive tool widely used in herbal medicine. The proposed collection contains all the necessary substances, the combined use of which has an effective influence on the animal organism in respiratory diseases.

Declared the collection has a bronchodilatory effect, promotes discharge of phlegm, anti-inflammatory and soothing effect, exhibits antiviral and antimicrobial activity, helps to restore the functions of the pulmonary system, has detoxifying properties, regulates metabolism and increases the body's resistance.

Grass and inflorescences of Echinacea purpurea possess immunomodulatory activity, inhibit the activity of hyaluronidase, the enzyme that enhances the permeability for viruses and microbes, increase the antimicrobial activity of leukocytes, macrophages and neutrophils in the blood and increase the synthesis of protective antibodies and endogenous interferon.

Grass and inflorescences mother and stepmother have a coating effect on the mucous membrane of the mouth, throat and larynx, protecting them from irritation (due to the presence of mucus), soften and liquefy the dry discharge is in the upper respiratory tract, restore the natural movement of the ciliated epithelium in the trachea and bronchi, contribute to a more rapid evacuation of the products of inflammation and significantly improve the expectoration (due to the presence of mucus, saponins, organic acids), have a pronounced anti-inflammatory effect, manifested by a reduction of hyperemia of the mucous membranes, and bacteriostatic properties.

Herb Rue ordinary has a pronounced antimicrobial activity, confirmed pharmacological research, provides bronchodilatory effect collection, promotes the discharge of mucus.

The roots of the licorice provide bronchodilators and anti-inflammatory effect collection. In addition, they enhance the healing effects of other herbs included in the collection.

The chemical composition of the treatment-and-prophylactic means is represented by a complex of biologically active substances, which ensures its high therapeutic effect. The predominant biologically active substances are phenolic compounds, flavonoids, polysaccharides, alkaloids, tannins, vitamins, minerals, the main of which can be considered as zinc, selenium, copper, and iron.

Flavonoids (contain all components) inhibit the enzyme catechol-ortho-methyltransferase and provide adaptation trophy the definition function of the body due to adrenoblokirutego effect. Flavonoids have P-vitamin (capillary) activity. In total anti-inflammatory effect of flavonoids is their direct effect on cell and tissue membranes - reducing their permeability, sealing membranes of lysosomes, inhibits hyaluronidase, an antagonistic effect against inflammation mediators /8/.

Polysaccharides contain all components) have anti-inflammatory, immunocorrigirutee action. They stimulate nonspecific immunity and phagocytosis activity of macrophages and neutrophils, as well as bactericidal function of cells.

Phenolic compounds (contain all components) have an antimicrobial effect, the mechanism of which is due to their ability to absorb components of the cytoplasmic membrane of bacteria to form strong hydrogen bonds with proteins and damage the membrane, in particular increasing its permeability to ions (especially potassium) and important metabolites, lost cell, as well as for the water coming from the outside and promotes lysis.

78% of the total number of alkaloids contained in the herb Rue ordinary, is paganin. Pharmacological studies showed that 1%and 10%grass infusions of Rue ordinary have high protivostoyaschej activity, and of 0.25%shall actor of Paganini - high antimicrobial activity.

Tannins (Glycyrrhiza glabra - to 9.46%, the mother-and-stepmother - not less than 8.0%), showing a stabilizing effect, restore repair processes in hepatocytes and improve the functioning of the antioxidant system of the liver.

Vitamins (vitamin C herb and inflorescences mother-and-stepmother, Echinacea, vitamin B - licorice, vitamin a, Echinacea purpurea) are involved in all processes of metabolism, increase the body's resistance to diseases, anti-inflammatory action, promote detoxification.

The trace element zinc (Glycyrrhiza glabra is 0.33 µg/g, Rue ordinary - 0.78 µg/g) participates in the body's immune processes, selenium (Glycyrrhiza glabra - 12,14 µg/g, Rue ordinary - 1.0 microgram/g, the mother-and-stepmother - to 22.9 µg/g) is a powerful antioxidant, iron (Echinacea - 48 mg/100 g, Rue ordinary - 0.14 mg/g, licorice - 0.7 mg/g) as a main structural component of hemoglobin and heme-containing enzymes, and in complex with cobalt (Glycyrrhiza glabra - 0,03 µg/g, Rue ordinary to 0.4 µg/ g, the mother-and-stepmother - 4.0 µg/g) has a stimulating effect on the immunogenesis in viral infections, copper (mother-and-stepmother - to 43.3 µg/g, licorice - 0.2 ug/g, Rue ordinary - 0.37 mcg/g) increases the functional activity of leukocytes, bactericidal what the authors estimated the effect activity of serum.

All medicinal plants possess antimicrobial activity, and the grass and clusters of purple coneflower - neutralizing property, kidney pine prevent the spread of viral infection in the body, eliminating (reducing) the causes of disease, anti-inflammatory (mother-and-stepmother, Echinacea), expectorant (mother-and-stepmother, Glycyrrhiza glabra), antispasmodic (Rue ordinary, the mother-and-stepmother) effect, reduces the pathogenetic changes, and the immunomodulatory activity of Echinacea enhances the protective, compensatory-adaptive mechanisms of an organism.

Tincture of the chopped vegetable mixture of grass and blossoms of Echinacea purpurea, the mother-and-stepmother, grass Rue ordinary and the roots of the licorice preparing pharmacopoeial method in the ratio of raw material to izvlekali 1:10. As sulcatas use ethanol concentration of 70%. Medicinal raw material is crushed to a particle size of 1-3 mm, mixed in equal quantities, a portion of the raw material is placed in a glass vessel, add the calculated amount of ethanol concentration of 70%, incubated 7 days in a dark place at a temperature of 15-20°C. Then alcohol tincture filter through a canvas or two layers of cheesecloth, pressing raw filter.

Example: in the glass vessel was placed 500 g Lek is stannage raw materials (an equal number from the grass and blossoms of purple coneflower, mother-and-stepmother, grass Rue ordinary and licorice root), added in 5.0 l of ethanol concentration of 70%, put in a dark place and kept at a temperature of 15-20°C for 7 days, after which it was filtered through the canvas, pressing the raw material, and was filtered.

For the prevention of respiratory disease in calves viral and bacterial etiology in the background of passive immunization against parainfluenza virus-3 (PG-3), infectious bovine rhinotracheitis (RTIs) and viral diarrhea-diseases of the mucous membranes (BD-BS) apply therapeutic and prophylactic representing an alcoholic tincture of the chopped vegetable mixture of grass and blossoms of Echinacea purpurea, the mother-and-stepmother, grass Rue ordinary and roots of licorice, taken in equal quantities, and for passive immunization - hyperimmune serum containing specific antibodies to the viruses, PG-3, RTI and VD-BS in titles not lower than 1:1280, 1:256 1:1024, respectively.

A method of preventing respiratory disease of calves with the use of therapeutic and prophylactic substances, representing an alcoholic tincture of the chopped vegetable mixture of grass and blossoms of Echinacea purpurea, the mother-and-stepmother, grass Rue ordinary and roots of licorice, taken in equal amounts, on a background of passive immunization is carried out as follows.

70% alcohol tincture of the ISM is Lannoy vegetable mixture of grass and blossoms of purple coneflower, mother-and-stepmother, grass Rue ordinary and the roots of the licorice in the form of 7-8% aqueous solution prepared in cooled boiled water, used orally in the dose of 1.5-2.0 ml/kg body weight of the animal within 15 days with an interval of 12-24 hours in addition to subcutaneous injection of hyperimmune serum containing specific antibodies to the viruses, PG-3, RTI and VD-BS in the credits not less than 1:1280, 1:256 1:1024, respectively, at a dose of 1.0 ml/kg of animal body weight three times with an interval in 10 days.

Effective prevention of respiratory disease of calves with the use of therapeutic and prophylactic substances, representing an alcoholic tincture of the chopped vegetable mixture of grass and blossoms of Echinacea purpurea, the mother-and-stepmother, grass Rue ordinary and roots of licorice, taken in equal amounts, on a background of passive immunization against viruses PG-3, RTI and VD-BS identified in the research-and-production experiments in conditions of households, the long-term affected with respiratory disease of young animals due to exposure to associations of viruses, pathogenic and conditionally pathogenic microflora in the background of violations of the conditions of detention of pregnant cows and calves. Virological and bacteriological studies confirmed the circulation of viruses PG-3 (85,6%), RTIs and VD-FV (57,5%), pasteurellosis, Salmonella, Proteus and is treptococcus. The incidence is 50%, the mortality rate of 15-20%. Clinically the disease is characterized by increased body temperature to 41.5°, pulse frequency up to 139 beats in 1 min and respiration up to 80 breaths 1 min, hyperemia of the mucous membranes covering the nasal speculum serous-mucous effusions of the nasal holes and eyes. The forced killed calves during acute flow in the lungs was found dense regions of red color, signs of enfisema, mucopurulent exudate in the trachea and bronchi, the signs of catarrhal hemorrhagic gastroenteritis. Zapotocny and bronchial nodes are enlarged.

The essence of the method is illustrated by examples.

Example 1. Prophylactic efficacy depending on the dose of treatment-and-prophylactic means in accordance with the invention was determined in clinically healthy calves 20-30 days of age, distributed in 5 groups: 4 groups - experienced group 1 - control. Calves in all groups were subcutaneously injected with hyperimmune serum containing specific antibodies to the viruses, PG-3, RTI and VD-BS in the credits not less than 1:1280, 1:256 1:1024, respectively, at a dose of 1.0 ml/kg of animal body weight three times with an interval of 10 days. The calves of the experimental group additionally used inside the claimed means in the form of a 7% aqueous solution for 15 days with an interval of 24 hours at doses of 1.0, 1.5 a, 2,0, 2.5 ml/kg body weight of the animal. Analysis was performed according to the main clinical characteristics (table 1). The results of the research (table 1) confirmed the validity of the proposed dosage is 1.5-2.0 ml/kg body weight of the animal. Doses below the minimum and above the maximum economically feasible.

Table 1
The effectiveness of the prevention of respiratory disease in calves, depending on the dose of therapeutic and preventive tool
IndicatorsThe way to prevent
inside the infusion of herbs and blossoms of Echinacea purpurea, the mother-and-stepmother, grass Rue ordinary and roots of licorice, taken in equal quantities, with an interval of 24 hours at a dose ml/kg body weightsubcutaneously hyperimmune serum* at a dose of 1.0 ml/kg body weight three times with an interval of 10 days n=40 (control)
1,01,52,02,5
and subcutaneously hyperimmune serum* at a dose of 1.0 ml/kg body weight three times with an interval of 10 days
n=45n=45n=42n=42
Sick goal.95449
Prophylactic efficacy, %80,088,590,590,577,5
Form parabolani, %
light73332
heavy22117
Palo goal.2---Safety, %95,6100,0100,0100,090,0
* hyperimmune serum contains specific antibodies to viruses PG-3, RTI and VD-BS in the credits not less than 1:1280, 1:256 1:1024, respectively

Example 2. The effectiveness of prophylaxis with the use of therapeutic-prophylactic means in accordance with the invention in comparison with known methods defined in clinically healthy calves 20-30 days of age, distributed in 4 groups. Calves in all groups were subcutaneously injected with hyperimmune serum containing specific antibodies to the viruses, PG-3, RTI and VD-BS in the credits not less than 1:1280, 1:256 1:1024, respectively, at a dose of 1 ml/kg of animal body weight three times with an interval of 10 days, additionally inside was used for 15 days with an interval of 24 hours: 1st group - treatment-and-prophylactic means in accordance with the invention in the form of a 7% aqueous solution at a dose of 2.0 ml/kg body weight of the animal; 2nd group - water extract of the herb of Hypericum perforatum, creeping thyme, peppermint and mother-and-stepmother, taken in equal quantities, at a dose of 100 ml, 3-I group - infusion of medicinal herbs include what it nettle, the burdock, camomile, marjoram common St. John's wort, common wormwood, plantain, mother-and-stepmother, oats, taken in equal quantities, at a dose of 300 ml 4-th group of additional drugs were not used. The animals were clinically observed for months. The results are presented in table 2.

Studies have shown that therapeutic tool has a variety of pharmacological activity, significantly increases the redox processes in the body, activates a number of enzyme systems, stimulates the body's resistance calves harmful effects of the external environment, infections and other adverse factors. Therapeutic and prophylactic agent is non-toxic, contains bioavailable useful biologically active substance, and its use has preventive and has a positive effect in the period of the disease.

Sources of information

1. Danilevsky V.M. Internal non-infectious diseases of farm animals. - M.: Agropromizdat, 1991. - P.148.

2. Scientific transactions. ASHI. - Omsk, 1987. - S.

3. Quick reference veterinarian / Altuhov NM, Afanasyev V.I., Bashkirov B.A. and others - M.: Agropromizdat, 1990. - S-369.

4. Veterinary medicine, No. 4,S.11-13.

5. EN 2250110 C1, 2005.

6. EN 2287994, 2006.

1. Therapeutic and prophylactic for young farm animals, representing an alcoholic tincture of plant material, characterized in that the quality of plant raw material, a mixture of equal amounts of herbs and blossoms of Echinacea purpurea, the mother-and-stepmother, grass Rue ordinary and roots of licorice, herbal raw material is poured in ethyl alcohol 70%concentration at a ratio of 1:10, incubated in the dark at a temperature of 15-20°C for 7 days, filtered, pressing raw and filtered.

2. A method of preventing respiratory disease of calves, including passive immunization, characterized in that the passive immunization perform subcutaneous injection of hyperimmune serum containing specific antibodies to the viruses, PG-3, RTI and VD-BS in the credits not less than 1:1280, 1:256 1:1024, respectively, at a dose of 1.0 ml/kg of animal body weight three times with an interval of 10 days and additionally inside apply therapeutic and prophylactic agent according to claim 1, in the form of an aqueous solution of 7-8% concentration in a dose of 1.5-2.0 ml /kg of animal body weight for 15 days with an interval of 12-24 hours



 

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14 cl, 14 tbl, 171 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to new compounds of formula (I) as (S)-stereoisomers or to their pharmaceutically acceptable salts which exhibit properties of MMP12 or MMP9 inhibitor. In formula (I) , R1 represents C1-2alkyl, cyclopropyl, OCH3 or SCH3, and said alkyl group is optionally substituted with one or more than one fluorine atom, and R2 represents C1-2alkyl.

EFFECT: invention also refers to methods for making compounds of formula (I), to the pharmaceutical composition and the method for making thereof, and also to application of the compounds of formula (I).

17 cl, 1 tbl, 6 ex

FIELD: chemistry.

SUBSTANCE: invention relates to novel therapeutically suitable derivatives of pyridazin-3(2H)-one of formula and pharmaceutical compositions containing the said derivatives. These compounds are used for treating, preventing or inhibiting corresponding pathological conditions, diseases or disorders, mainly asthma, chronic obstructive pulmonary disease, rheumatoid arthritis, atopic dermatitis, psoriasis or irritable colon syndrome.

EFFECT: obtaining compounds which are active and selective phosphodiesterase 4 (PDE4) inhibitors.

11 cl, 1 tbl, 182 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to pulmonology, and can be used in treatment of remitted chronic obstructive pulmonary disease. That is ensured by intravenous drop-by-drop introduction of 1.5% Reamberin in the volume of 400 ml included in the therapy, 5 injections every second day.

EFFECT: Reamberin therapy in specially developed regimen allows improving respiratory function and cardiac-pulmonary hemodynamics in the given category of patients and ensuring substantial prolongation of remission due to manifested antihypoxic activity of Reamberin.

2 tbl, 1 ex

FIELD: medicine, veterinary science.

SUBSTANCE: treatment-and-prophylactic agent for young farm animals represents an alcoholate of milled vegetable mixture of purple coneflower herb and blossom clusters, Syrian rue herb, tillet blossom and licorice roots in equal amounts. Prevention of respiratory diseases in calves with underlying passive immunisation is ensured by subcutaneous injection of a hyperimmune serum containing specific antibodies to viruses PG-3, IRT and VD-BS in titres not less than 1:1280, 1:256 and 1:1024 respectively in a dose 1.0 ml/kg of an animal's live weight, threefold every 10 days, with intake of the treatment-and-prophylactic agent representing alcoholate of milled vegetable mixture of purple coneflower herb and blossom clusters, Syrian rue herb, tillet blossom and licorice roots taken in equal amopunts, in the form of 7-8% aqueous solution in a preventive dose 1.5-2.5 ml/kg of the animal's live weight every 12-24 hours during 15 days.

EFFECT: invention provides improved effectiveness of prevention.

2 cl, 2 tbl, 2 ex

FIELD: medicine, veterinary science.

SUBSTANCE: invention concerns veterinary medicine, namely to agents prepared from medical plants. The invention consists in the following. Mixture of the following medical plants crushed to fine and ultrafine particles and treated with bactericidal lamps rays are placed in a sterile tank of dark glass: dry stalks, leaves and flowers of peppermint, dry flowers of wild chamomile, dry leaves of waybread and dry flower heads of costmary (Tanacetum vulgare), dry stalks, leaves and flowers of yellow melilot, dry grass of St. John's wort, dry flowers of pot marigold, dy grass of milfoil, dy grass of thyme, dry stalks, leaves, flowers and fruits of celandine, dry leaves of eucalyptus, dry leaves and flower heads of coltsfoot, dry leaves of garden sage, dry leaves of stinging nettle, dry grass of common persicaria, dry flowers of densely flowered mullein, bark of ordinary oak, roots of common licoric, ground fruits of anise and rose hips, walnut kernel, buckthorn pulp without kernels, their fruits and berries. These components are treated with bactericidal lamps rays and crushed to fine and ultrafine particles, then placed to a tank of dark glass, mixed with sterile cooking salt solution with 0.9% sodium content, and with formalin. The mixture is left and room temperature for 3-4 days, regularly stirred, and then placed to water bath for 10-12 hours and exposed to temperature up to 40°, with contiuous stirring. After that the liquid part is separated from the vegetable sediment. The tincture is stored in tanks of dark glass or in a darkened room at a temperature of 2-4°C, and is applied externally and internally, and the vegetable sediment is used as fodder and remedy for animals and birds, especially for chickens in case of cannibalism.

EFFECT: at experimental checks of therapeutic efficacy of the agent, good results were achieved in prevention and treatment of inflammatory processes among animals; wide application in veterinary.

3 cl

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