Composition containing enzyme and toned agent for fabric

FIELD: chemistry.

SUBSTANCE: present invention relates to compositions which contain certain versions of lipase and a toned agent for fabric, and includes use of such compositions for cleaning and/or treating surface areas or fabric.

EFFECT: improved deposition of temporary colourant, reduced activity of the enzyme which leads to bad smell, and improved perception of cleanness.

17 cl, 3 tbl, 16 ex

 

The technical field

The invention relates to compositions containing lipase and colouring agents for fabrics and methods of producing and using such products.

Prior art

The emergence of lipase enzymes suitable for use in detergents, the developer creates a new opportunity to improve removal of fat. These enzymes catalyze the hydrolysis of triglycerides, which constitute the major component of many widespread grease, such as the secretion of sebaceous glands, animal fats (e.g. lard, melted fat, oil)and vegetable oil (e.g. olive oil, sunflower oil, peanut oil). However, these enzymes typically have low efficiency in the first cycle of washing and typically lead to the formation of unpleasant odors arising are considered to be the result of hydrolysis of fats present in the stains from dairy products such as milk drinks, cream, butter and yogurt. Without being limited to theory, it is believed that these impurities have a tendency to the formation of induced lipase unpleasant smell, as they contain triglycerides, functionalityand short-chain (e.g., With4) fatty acyl chains that after lipolysis release malodorous volatile fatty acids. Even if the improvement of the effectiveness of these enzymes issue odor remains. Thus, the use of this technology is severely limited.

We found that the combination of colouring agent for fabrics with certain variants of the lipase leads to improvement of the treatment efficiency at the same time minimizing unacceptable odor. Not wishing to be bound by theory, we can note that, as is, these beneficial effects are likely to be provided by the following mechanisms: selected options lipase increase the degree of fat removal that leads to improved access colouring agent to the tissue with the tissue surface and, consequently, its improved deposition. The resulting combination of improved removal of oil contaminants and sediments tinting dye lead to an improvement in the appearance of the fabric; even in case of insufficient removal of oil pollution, the hydrolysis of fats to more hydrophilic fatty acids, mono - and diglycerides leads to improved deposition tinting dye and, thus, the feeling of cleanliness; presence of dye molecules deposited in the oil impurities present on the fabric, can inhibit the activity of the enzyme, leading to the occurrence of an unpleasant smell.

The invention

This invention relates to compositions containing lipase and colouring agents for fabrics and methods of producing and using the same the products.

A detailed description of the invention

Definition

Used here, the term "cleaning composition" includes, unless otherwise indicated, granular or powder-like universal or "for work in difficult conditions" detergents, especially detergents for Laundry use; liquid, gel or paste-like universal cleaning agents, especially the so-called funds liquid type for heavy-duty, liquid detergent for delicate lung tissue; means for washing dishes manually or dishwasher tools for lightweight modes, especially silvopastures type; means for dishwashing machines, including the various tablet, granular, liquid types and means to reduce the number of remaining on the dishes drops for use in the home and in catering establishments; liquid cleaning and disinfecting agents, including antibacterial agents for hand washing, lump detergents, liquid for rinsing the mouth, cleanser for the teeth and mouth, shampoos for washing machines and carpet cleaning products bathroom; shampoos and conditioners for the hair; shower gels and foam for the soul and the means for cleaning metals; as well as additives for detergents, such as bleach additives and "pencils for removing stains or means for preventing the provisional processing.

Used here meaning the term "toning agent for fabric" means the dyes or pigments which, when incorporated in a detergent composition may be deposited on the fabric when the specified fabric is introduced into contact with the detergent solution containing the detergent composition, thereby changing the hue of the specified fabric. For the purposes of this application fluorescent optical brighteners are not considered colouring agents for fabrics.

In used here in the meaning, the phrase "independently selected from the group consisting of..." means that the components or elements selected from the specified group Markush, may be the same, may be different or may be any mixture of elements.

Methods of testing disclosed in the section methods of testing this application should be used to determine the appropriate values of the parameters of the claimed inventions.

Unless otherwise indicated, all levels of the components or compositions include the active levels of a given component or composition, and do not take into account impurities, such as residual solvents or by-products that may be present in commercially available sources.

All values are percentages and ratios are calculated by weight, unless otherwise indicated. All values are percentage and sootnoshenie is calculated with respect to the whole composition, if not specified otherwise.

It should be understood that every maximum numerical limit specified in the present description includes every lower numerical limit, as if such lower numerical limits were explicitly listed here. Each minimum numerical limit specified in the present description, will enable each of the upper numerical limit, as if such upper numerical limit has been explicitly specified here. Each interval of numerical values specified in this specification will include every narrower interval of numerical values within this broader interval of numerical values, as if such narrower intervals of numerical values were all explicitly specified here.

All cited documents, in relevant part, incorporated here as a reference; a reference to any document should not be construed as an admission that it is prior art with respect to the present invention.

Composition

The compositions of the present invention typically contain from about 0,00003% to about 0.1%, from about 0,00008% to approximately 0.05%, or even from about 0,0001% to about 0.04% of the colouring agent for fabric and from some of 0.0005% to about 0.1%, from about 0,001% to about 0.05 percent, or even from about 0.002% to about 0.03% of the lipase.

Such compositions can have the ü any form, for example, the form of washing compositions and/or compositions to be processed.

The rest of detergent compositions according to any one of the above aspects consists of one or more auxiliary materials.

Suitable lipase

Suitable lipases include lipases selected from the group consisting of lipases belonging to the group AS Classification of enzymes, and mixtures thereof. In another aspect, suitable lipases include lipases selected from the group consisting of lipases, classified AS, as defined by the EU classification, IUPAC-IUBMB, and mixtures thereof.

Examples of lipase EC 3.1.1.3 include described in WIPO publications WO 00/60063, WO 99/42566, WO 02/062973, WO 97/04078, WO 97/04079 and US 5869438. Preferred lipases are produced by Absidia reflexa, Absidia corymbefera, Rhizmucor miehei, Rhizopus delemar, Aspergillus niger, Aspergillus tubigensis, Fusarium oxysporum, Fusarium heterosporum, Aspergillus oryzea, Penicilium camembertii, Aspergillus foetidus, Aspergillus niger, Thermomyces lanoginosus (synonym: Humicola lanuginosa) and Landehna penisapora, especially Thermomyces lanoginosus. Some of the preferred lipases are supplied by the company Novozymes under the trade names Lipolase®, Lipolase Ultra®, Lipoprime®and Lipex®(registered trademarks of Novozymes), and LIPASE P "AMANO®available from Areario Pharmaceutical Co. Ltd., Nagoya, Japan, AMANO-CES®, commercially available from Toa Jozo Co., Tagata, Japan; and other lipase Chromobacter viscosum from US Biochemical Corp., USA and Diosynth Co., Netherlands, and other lipases, such as Pseudomonas gladioli. The l is further suitable lipases described in WIPO publications WO 02062973, WO 2004/101759, WO 2004/101760 and WO 2004/101763. In one embodiment, the embodiment is suitable lipases include lipases first cycle"described in WO 00/60063 and U.S. patent 6939702 B1, preferably a variant of SEQ ID NO:2, preferably a variant of SEQ ID NO:2 having at least 90% homology with SEQ ID NO:2 and containing a substitution of an electrically neutral or negatively charged amino acid at R or at any of positions 3, 224, 229, 231 and 233, and the most preferable variant contains a mutation T231R and N233R, and this most preferred option is sold under the brand name Lipex®. The above-mentioned lipase can be used in combination (can be used any mixture of lipase). Suitable lipases can be purchased from Novozymes, Bagsvaerd, Denmark; Areario Pharmaceutical Co. Ltd., Nagoya, Japan; Toyo Jozo Co., Tagata, Japan; Amersham Pharmacia Biotech., Piscataway, New Jersey, USA; Diosynth Co., Oss, Netherlands and/or manufactured in accordance with the examples in the present invention.

Suitable colouring agents for fabric

Fluorescent optical Brightener emit at least some amount of visible light. In contrast, colouring agents for fabrics can change the shade of the surface, because they absorb at least part of the spectrum of visible light. Suitable colouring agents for fabrics include dyes, conjugates dye-clay and pigments that meet the requirements of the test Method 1 in section test Methods of the present description. Suitable dyes include the dyes with small molecules and polymeric dyes. Suitable dyes with small molecules include dyes with small molecules selected from the group consisting of dyes falling under the classification of the Colour Index (CI) direct blue, direct red, direct violet, acid blue, acid red, acid violet, basic blue, basic violet and basic red, or mixtures thereof, for example:

(1) Tris-azo direct blue dyes of the formula

where at least two of naftalina rings, and substituted sulphonate group, the ring may be substituted in position 5 by a group NH2or NHPh, X denotes benzyl or naphtalene ring, substituted up to 2 sulphonate groups and may be substituted in position 2 by a group of IT, and may also be substituted by a group NH2or NHPh.

(2) Bis-azo direct violet dyes of the formula:

where Z denotes H or phenyl, ring And, preferably, substituted methyl and methoxy group in the positions indicated by the arrows, ring And can also be nafcillin ring, the group Y is benzyl or nafcillin ring, which is substituted by sulfate groups and may be mono - or disubstituted by methyl groups.

(3) Blue or red to the PCI-e slot dyes of the formula:

where at least one of X and Y must be an aromatic group. In one aspect both aromatic groups can be substituted benzyl or naftilos group which may be substituted is not solubilities in water groups such as alkyl or alkyloxy or alloctype, X and Y cannot be substituted solubilities in water groups, such as sulfonates or carboxylates. In another aspect X is nitrosamines benzyl group, and Y denotes a benzyl group.

(4) Red acid dyes patterns

or

where is naftilos or benzyl group which may be substituted is not solubilities in water groups such as alkyl or alkyloxy or alloctype, cannot be substituted solubilities in water groups, such as sulfonates or carboxylates.

(5) Dis-azo dyes of the structure

or

,

where X and Y independently of one another, represent each hydrogen, C1-C4-alkyl or C1-C4-alkoxy, Rαdenotes hydrogen or aryl, Z represents C1-C4-alkyl, C1-C4-alkoxy; halogen; hydroxyl or carboxyl, n is 1 or 2 and m is 0, 1, and 2, and their respective salts and mixtures thereof;

(6) Triphenylmethane dyes of the following structures

and/or

and mixtures thereof. In another aspect suitable dyes with small molecules include dyes with small molecules selected from the group consisting of Colour Index (Society of Dyers and Colourists, Bradford, UK) direct violet 9, direct violet 35, direct violet 48, direct violet 51, direct violet 66, direct blue 1, direct blue 71, direct blue 80, direct blue 279, acid red 17, acid red 73, acid red 88, acid red 150, acid violet 15, acid violet 17, acid violet 24, acid violet 43, acid violet 49, acid blue 15, acid blue 17, acid blue 25, acid blue 29, acid blue 40, acid blue 45, acid blue 75, acid blue 80, acid blue 83, acid blue 90 and acid blue 113, acid black 1, basic violet 1, basic violet 3, basic violet 4, basic violet 10, basic violet 35, basic blue 3, basic blue 16, basic sineg is 22, basic blue 47, basic blue 66, basic blue 75, basic blue 159, and mixtures thereof. In another aspect suitable dyes with small molecules include dyes with small molecules selected from the group consisting of Colour Index (Society of Dyers and Colourists, Bradford, UK) No. of acid violet 17, acid violet 43, acid red 73, acid red 88, acid red 150, acid blue 25, acid blue 29, acid blue 45, acid blue 113, acid black 1, direct blue 1, direct blue 71, direct violet 51, and mixtures thereof. In another aspect suitable dyes with small molecules include dyes with small molecules selected from the group consisting of Colour Index (Society of Dyers and Colourists, Bradford, UK) acid violet 17, direct blue 71, direct violet 51, direct blue 1, acid red 88, acid red 150, acid blue 29, acid blue 113, or mixtures thereof.

Suitable polymeric dyes include polymeric dyes selected from the group consisting of polymers containing conjugated Chromogens (conjugates of the dye-polymer), and polymers with Chromogens, copolymerizable in the main chain of the polymer, and mixtures thereof.

In another aspect, suitable polymeric dyes include polymeric dyes selected from the group consisting of substantive cu the bearers for fabrics, sold under the name Liquitint®(Milliken, Spartanburg, South Carolina, USA), conjugates of the dye-polymer formed from at least one reactive dye and a polymer selected from the group consisting of polymers comprising a fragment selected from the group consisting of hydroxyl groups, primary amino groups, secondary amino groups, Tilney groups and mixtures thereof. In yet another aspect, suitable polymeric dyes include polymeric dyes selected from the group consisting of Liquitint®(Milliken, Spartanburg, South Carolina, USA) violet CT, carboxymethylcellulose (CMC), conjugated with reactive blue, reactive violet or reactive red dye, such as CMC, conjugated with C.I. reactive blue 19, which is sold by the company Megazyme, Wicklow, Ireland under the name AZO-CM-CELLULOSE, product code S-ASMS, and mixtures thereof.

Suitable conjugates dye-clay include conjugates dye-clay selected from the group consisting of at least one cationic/basic dye and smectite clay and mixtures thereof. In another aspect suitable conjugates dye-clay include conjugates dye-clay selected from the group consisting of one cationic/basic dye selected from the group consisting of basic yellow C.I. 1-108, basic orange C.I. 1-69, basic red C.I. 1-118, the main Phi is letovah C.I. 1-51, basic blue C.I. 1-164, basic green C.I. 1-14, basic brown C.I. 1-23, basic black C.I. 1-11, and clay selected from the group consisting of montmorillonite clay, hectorite clay, aponitolau clay and mixtures thereof. In another aspect suitable conjugates dye-clay include conjugates dye-clay selected from the group consisting of conjugate montmorillonite is the main blue V7 C.I. 42595, conjugate, montmorillonite-basic blue B9 C.I. 52015, conjugate, montmorillonite is the main purple V3 C.I. 42555, conjugate, montmorillonite is the main green G1 C.I. 42040, conjugate, montmorillonite is the main red R1 C.I. 45160, conjugate, montmorillonite-C.I. basic black 2, conjugate hectorite-primary blue V7 C.I. 42595, conjugate hectorite-basic blue B9 C.I. 52015, conjugate hectorite-basic violet V3 C.I. 42555 the conjugate hectorite is the main green GI C.I. 42040, conjugate hectorite-basic red R1 C.I. 45160, conjugate hectorite-C.I. basic black 2, conjugate, saponite-primary blue V7 C.I. 42595, conjugate, saponite-basic blue B9 C.I. 52015, conjugate, saponite-basic violet V3 C.I. 42555, conjugate, saponite is the main green G1 C.I. 42040, conjugate, saponite-basic red R1 C.I. 45160, conjugate, saponite - C.I. basic black 2, and mixtures thereof.

Suitable pigments include pigments selected from the group consisting of flavanone, indanthrene, chlorinated and dantron, containing from 1 to 4 chlorine atoms, piraterna, dichloropentane, monobromobimane, dibromodichloromethane, tetrabromoethane, diimide perylene-3,4,9,10-tetracarbonyl acid, where kidnie groups can be unsubstituted or substituted C1-C3-alkyl or phenyl or heterocyclic radical, and where the phenyl and heterocyclic radicals may optionally carry substituents which do not impart solubility in water, amides intraperitoneally acid, violation, isofilename, dioxazine pigments, copper phthalocyanine, which may contain up to 2 chlorine atoms in the molecule, polihlorvinila copper or peribronchovascular copper containing up to 14 atoms of bromine in the molecule, and mixtures thereof. In another aspect suitable pigments include pigments selected from the group consisting of ultramarine blue (C.I. pigment blue 29), ultramarine violet (C.I. pigment violet 15) and mixtures thereof.

The above colouring agents for fabrics can be used in combination (can be any mixture of colouring agents for fabric). Suitable colouring agents for fabrics can be purchased from Aldrich, Milwaukee, Wisconsin, USA; Ciba Specialty Chemicals, Basel, Switzerland; BASF, Ludwigshafen, Germany; Dayglo Color Corporation, Mumbai, India; Organic Dyestuffs Corp., East Providence, Rhode Island, USA; Dystar, Frankfurt, Germany; Lanxess, Leverkusen, Germany; Megzyme, Wicklow, Ireland; Clariant, Muttenz, Switzerland; Avecia, Manchester, UK and/or manufactured in accordance with the examples in the present invention.

Auxiliary materials

Although it is not essential for the purposes of the present invention, non-limiting list of excipients described here and further is suitable for use in the compositions of the present invention and may be desirable for inclusion in some of the variants of embodiment of the invention, for example to ensure or improve the treatment efficiency of the processing of the substrate in need of cleaning, or for modification of the aesthetic characteristics of the detergent, as in the case of perfumes, pigments, dyes, etc. as to the Exact nature of these additional components, and levels of their content will depend on the physical form of the composition and nature of the cleaning operation for which they should be used. Suitable support materials include, without limitation, surfactants, core components, chelating agents, agents inhibiting the transfer of dyes, dispersants, additional enzymes, and enzyme stabilizers, catalytic materials, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, pre-prepared percolate, polymeric dispersants, agents for UD is of dirt particles and prevent them from re-deposition, clarifiers, pinagsama additives, dyes, odorants, sastifactory patterns, fabric softeners, carriers, girotropnye substances, processing AIDS, solvents and/or pigments. In addition to the following description suitable examples of such other auxiliary substances and the levels of their use are shown in U.S. patent No. 5576282, 6306812 B1 and 6326348 B1, which are incorporated here as reference.

As mentioned, the auxiliary ingredients are not essential to the inventive compositions. Thus, in certain variations of embodiments of the inventive compositions do not contain one or more of the following materials, auxiliary chemicals: surfactants, core components, chelating agents, agents inhibiting the transfer of dyes, dispersants, additional enzymes, and enzyme stabilizers, catalytic materials, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, pre-prepared percolate, polymeric dispersants, agents to remove dirt particles and prevent them from re-deposition, brighteners, pinagsama additives, dyes, odorants, sastifactory patterns, fabric softeners, carriers, girotropnye substances, processing AIDS, solvents and/or pigments. However, in the presence of one or bol is more excipients may be present, such one or more excipients, the ones below.

Bleaching agents - detergents compositions of the present invention can include one or more bleaching agents. Suitable bleaching agents other than the bleach catalysts include fotoatelier, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, pre-prepared percolate and mixtures thereof. In General, in the case of a bleaching agent composition of the present invention may include from about 0.1% to about 50%, or even from about 0.1% to about 25% of the whitening agent to the weight of this detergent composition. Examples of suitable bleaching agents include:

(1) fotoatelier, for example, the sulfonated zinc phthalocyanine, sulfonated aluminum phthalocyanines, xanthene dyes, and mixtures thereof;

(2) pre-prepared percolate: suitable pre-prepared percolate include, without limitation, compounds selected from the group consisting of percarbonic acids and salts, peroralnyh acids and salts, perekidnyh acids and salts, peroxymonosulfuric acids and salts, such as Oxone®, and mixtures thereof. Suitable percarbonate acid include hydrophobic and hydrophilic percolate having the formula R-(C=O)O-O-M, where R denotes an alkyl group, optionally branched, containing, in those cases is Ah, when percolate is hydrophobic, from 6 to 14 carbon atoms or from 8 to 12 carbon atoms and, in those cases where percolate is hydrophilic, less than 6 carbon atoms or even less than 4 carbon atoms; M represents a counterion, such as sodium, potassium or hydrogen;

(3) sources of hydrogen peroxide, for example inorganic perpetrate salts, including alkali metal salts such as sodium salt of perborate (usually mono - or tetrahydrate), percarbonate, persulfate, perphosphate, perserikatan salts and mixtures thereof. In one aspect of the invention inorganic perpetrate salt selected from the group consisting of sodium salts of perborate, percarbonate and mixtures thereof. In the case of using inorganic perpetrate salt is typically present in an amount of from 0.05 to 40 wt.% or from 1 to 30 wt.% from the total amount of the composition and is typically included in such compositions in the form of a crystalline solid, which can have the floor. Suitable coatings include inorganic salts such as silicate, carbonate or borate salts of alkali metals or mixtures thereof, or organic materials such as water-soluble or dispergirujutsja polymers, waxes, oils or fatty Soaps;

(4) the bleaching activators of the formula R-(C=O)-L, where R denotes an alkyl group, optionally branched, contain asuu, in those cases, when the bleach activator is hydrophobic, from 6 to 14 carbon atoms or from 8 to 12 carbon atoms and, in those cases, when the bleach activator is hydrophilic, less than 6 carbon atoms or even less than 4 carbon atoms; L denotes outgoing group. Examples of suitable waste groups are benzoic acid and its derivatives, and especially bansilalpet. Suitable bleach activators include dodecanesulfonate, technologiessalon, technologiesin acid or its salts, 3,5,5-trimethylcyclohexylisocyanate, tetraacetylethylenediamine (TAED) and nonanoyloxybenzenesulfonate (NOBS). Suitable bleach activators are also disclosed in WO 98/17767. Although it may be used any suitable bleach activator, in one aspect of the invention which is the subject of the invention of the washing composition may include NOBS, TAED or mixtures thereof.

In the presence of percolate and/or bleach activator is typically present in the composition in an amount of from about 0.1 to about 60 wt.%, from about 0.5 to about 40 wt.%; or even from about 0.6 to about > 10% from the composition. One or more hydrophobic perkiset or its precursors can be used in combination with one or more hydrophilic percolate or its precursor.

The number of source is ercise hydrogen and percolate or bleach activator can be chosen in such a way, that the molar ratio of available oxygen (from the source of peroxide) to percolate is from 1:1 to 35:1 or even 2:1 to 10:1.

Surfactant - detergent compositions in accordance with the present invention may include a surfactant or surfactant, where the surfactant may be selected from nonionic surfactants, anionic surfactants, cationic surfactants, ampholytic surfactants, zwitterionic surfactants, polupryamykh nonionic surfactants and mixtures thereof. In the presence of surfactant is typically present in an amount of from about 0.1% to about 60%, from about 1% to about 50%, or even from about 5% to about 40 wt.%, from being the subject of the invention composition.

The main components of the cleaning compositions of the present invention may include one or more of the main components of the detergent or system main component. In the case of a main component which is the subject of the invention, the composition will typically include at least about 1%, from about 5% to about 60%, or even from about 10% to about 40%, a major component of the weight of the show is Asia the subject invention compositions.

The main components include, without limitation, polyphosphate salts of alkali metals, ammonium and alkanolamine, silicates of alkali metals, carbonates of alkaline earth and alkali metal aluminosilicate core components and polycarboxylate connection epigallocatechingallate, copolymers of maleic anhydride with ethylene or vinylmation ether, 1,3,5-trihydroxybenzene-2,4,6-trisulfonic acid and carboxymethylcysteine acid, various salts of alkali metals, ammonium and substituted ammonium polixeni acids, such as ethylenediaminetetraacetic acid, nitrilotriacetic acid, and polycarboxylate, such as malletova acid, succinic acid, citric acid, accidentally acid, primulina acid, benzene-1,3,5-tricarboxylic acid, carboxymethylcysteine acid and its soluble salts.

Chelating agents - the cleaning compositions of the present invention may contain a chelating agent. Suitable chelating agents include chelating agents, copper, iron and/or manganese, and mixtures thereof. In case of using a chelating agent which is the subject of the invention, the composition may include from about 0,005% to about 15%, or even from about 3.0% to about 10%, gelatinous agent by weight of the subject invention compositions.

Agents that inhibit the migration of the dye - detergent compositions of the present invention can also include one or more agents, inhibiting the migration of the dye. Suitable polymeric agents, inhibiting the transfer of dyes include, without limitation, polyvinylpyrrolidone polymers, polyamine N-oxide polymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polivinilatsetatny and polyvinylimidazole or mixtures thereof. In the case of the presence in the subject invention compositions of agents that inhibit the transfer of dye can be present in an amount of from about 0,0001% to about 10%, from about 0.01% to about 5%, or even from about 0.1% to about 3%, by weight of the composition.

Clarifiers - detergent compositions of the present invention may also contain additional components that may tint articles being washed, such as fluorescent brighteners. Suitable levels of fluorescent brighteners include from the lower levels of from about 0.01 to, from approximately 0.05, about 0.1, or even from about 0.2 wt.%, to the upper levels of 0.5 or even 0.75 wt.%.

The dispersant compositions of the present invention can also contain dispersants. Suitable water-soluble organic materials include Homo - or copolymer acid or their salts, in which polycarbonatediol includes at least two carboxyl radical, separated from each other by no more than two carbon atoms.

Enzymes - detergent compositions can include one or more enzymes which provide cleaning performance and/or fabric care. Examples of suitable enzymes include, without limitation, hemicellulase, peroxidase, protease, cellulase, xylanase, lipase, phospholipase, esterase, cutinase, pectinase, mannanase, pectolyase, keratinase, reductase, oxidase, phenoloxidase, lipoxidase, ligninase, pullulanase, tannaz, pentosanase, Malagasy, β-glucanase, arabinosidases, hyaluronidase, chondroitinase, laccase, and amylases, or mixtures thereof. A typical combination is an enzyme cocktail, which may contain, for example, protease and lipase in combination with amylase. In the case of presence in the detergent composition of the above-mentioned additional enzymes may be present in amounts from about 0.00001% to about 2%, from about 0,0001% to about 1%, or even from about 0,001% to about 0.5%, fermenting protein by weight of the composition.

Stabilizers enzymes - enzymes for use in detergents can be stabilized in different ways. The enzymes used in the present invention can be stabilized by the presence in the finished compositions of water-soluble sources of calcium ions and/or magnesium which provide the t delivery of such ions to the enzymes. In the case of aqueous compositions, containing a protease, a reversible protease inhibitor, such as a boron compound can be added for additional stability.

The catalytic metal complexes - the claimed detergent composition can include catalytic metal complexes. One type of metal-containing bleaching catalyst system is a catalyst comprising a transition metal cation with a specific catalytic activity whitening, such as the cations of copper, iron, titanium, ruthenium, tungsten, molybdenum or manganese, an auxiliary metal cation having little or no catalytic activity whitening, such as the cations of zinc or aluminum, and prescribe, having defined stability constants with the catalytic and auxiliary metal cations, particularly ethylenediaminetetraacetic acid, ethylenediaminetetra(methylenephosphonic acid) and their soluble salts. Such catalysts are disclosed in US 4430243.

Optionally, the compositions of the present invention can be metalizowany using manganese compounds. Such compounds and levels of use are well known in the art and include, for example, catalysts based on manganese disclosed in US 5576282.

Cobalt catalysts whitening suitable DL is used in this invention, known and described, for example, in US 5597936; US 5595967. Such cobalt catalysts easily get known methods, such as described, for example, in US 5597936 and US 5595967.

The compositions of the present invention is also suitable to include the complex of the transition metal with ligands, such as bispinosa (WO 05/042532 A1) and/or hard macropolicies ligands - abbreviated marked "MRLs". In practice, and without limitation, the compositions and methods of the present invention can be adjusted in order to provide at least one part per hundred million of the active particles MRL in the aqueous washing medium, and will typically provide from about 0,005 million-1to about 25 million-1from approximately 0.05 million-1up to about 10 million-1or even from about 0.1 million-1to about 5 million-1MRL in the washing solution.

Suitable transition metals in the bleaching catalyst based on transition metal of the present invention include, for example, manganese, iron and chromium. Suitable MRLs include 5,12-diethyl-1,5,8,12-tetraazabicyclo[6.6.2]hexadecane.

Suitable transition metal MRLs easily get known methods, such as described, for example, in WO 00/32601 and US 6225464.

Solvents suitable solvents include water and other solvents, such as lipophilic fluid. Examples of suitable lip is a multidisciplinary liquid media include siloxanes, other silicones, hydrocarbons, simple glycol ethers, derivatives of glycerol, such as ethers, glycerin, perfluorinated amines, perfluorinated and hydrotherapie solvents, low-volatile non-fluorinated organic solvents, diol solvents, other environmentally safe solvents and mixtures thereof.

Methods of obtaining compositions

The compositions of the present invention can be prepared in any suitable form and produced by any method chosen by the originator, non-limiting examples of which are described in the examples of the application and in the US 4990280; US 20030087791 A1; US 20030087790 A1; US 20050003983 A1; US 20040048764 A1; US 4762636; US 6291412; US 20050227891 A1; EP 1070115 A2; US 5879584; US 5691297; US 5574005; US 5569645; US 5565422; US 5516448; US 5489392; US 5486303, which are all included in the present invention as references.

Method of use

The present invention includes a method of cleaning and/or processing site, including, surface or fabric. This method includes the introduction phase in contact variant embodiment of the inventive washing compositions, undiluted or diluted in the washing solution, with at least part of a surface or fabric, and then optionally rinsing the surface or fabric. Surface or the fabric may be subjected to a stage of washing before the above-mentioned stage rinse. For the purposes of the present invention, the washing including the AET, without limitation, washing and mechanical stirring. As will be clear to experts in this field, the detergent compositions of the present invention are ideal for use when washing. Accordingly, the present invention includes a method of washing fabric. The method includes the introduction phase in contact with the fabric to be washed, with the specified detergent to the washing solution containing at least one variant embodiment of the inventive detergent compositions, detergent additive, or a mixture. The fabric can include almost any fabric, suitable for washing in normal conditions of use. The solution preferably has a pH from about 8 to about 10.5V. The composition can be used in concentrations of from about 500 million-1to about 15000 million-1in the solution. Water temperature typically ranges from about 5°to about 90°C. the Ratio of water:fabric typically is from about 1:1 to about 30:1.

TEST METHOD 1

Below is a Protocol designed to determine whether the dye or pigment material colouring agent for fabric purposes of the invention:

1) Fill two targetmetadata vessel of 800 ml of water from the municipal water supply Newcastle upon Tyne, UK (total hardness OK Grand Prix at the American gallon is supplied Northumbrian Water, Pity Me, Durham, Co. Durham, UK).

2) Set the vessel is in ergotimer at a given water temperature 30°C and stirring 40 rpm throughout the experiment.

3) is Added to each vessel 4.8 g of detergent IEC-B (IEC 60456 Reference basic detergent for washing machines of the type In) was delivered wfk, Briiggen-Bracht, Germany.

4) After two minutes, add 2.0 mg of active dye into the first vessel.

5) After one minute, add in each vessel 50 g of tissue from non-compacted cotton (supplied by Warwick Equest, Consett, County Durham, UK), cut into samples of 5 cm × 5 cm

6) After 10 minutes, pour the liquid from the vessel and again fill with cold water from the municipal water supply Newcastle upon Tyne (16°C).

7) After 2 minutes, rinse out the cloth.

8) Repeat stages 3-7 three more times using the same processing.

9) Gather the fabric and dry it on a clothesline in the room for 12 hours.

10) Analyze the samples using a spectrometer Hunter Miniscan equipped with light source D65 and a cut-off filter UVA, to obtain values of the Hunter (the axis of the red-green) and Hunter b (axis yellow-green).

11) Average values of Hunter and Hunter b for each set of tissues. If the fabric treated with the tested dye, show the average difference of hue more than 0.2 units of a-axis or b axis, it is considered toning agent for fabric purposes of the invention.

EXAMPLES

Unless otherwise specified, the materials can be obtained from Aldrich, P.O. Box 2060, Milwaukee, WI 53201, USA.

Examples 1-6

Granular HDMI is of detergents for washing, designed for hand washing or washing machines top loading.

0,6
Table 1
1 (wt.%)2 (wt.%)3 (wt.%)4 (wt.%)5 (wt.%)6 (wt.%)
Line Las202220152020
With12-14-dimethylhydroxylamine chloride0,7110,60,00,7
AE3S0,90,00,90,00,00,9
AE0,00,50,0131
Sodium tripolyphosphate233023171223
Zeolite And0,00,00,00,0100,0
1.6 has been released Silicate (SiO2-Na2O in the ratio of 1.6:1)777777
Sodium carbonate151415181515
Polyacrylate MW 450010,0111,51
Carboxymethylcellulose1111 11
Savinase®32,89 mg/g0,10,070,10,10,10,1
Natalase®8,65 mg/g0,10,10,10,00,10,1
Lipex®18 mg/g0,030,070,30,10,070,4
Fluorescent Brightener 10,060,00,060,180,060,06
Fluorescent Brightener 20,10,060,10,00,10,1
Diethylenetriaminepentaacetic acid0,60,30,250,60,6
MgSO41110,511
Percarbonate sodium0,05,20,10,00,00,0
Perborate sodium monohydrate4,40,03,852,090,783,63
NOBS1,90,01,66-0,330,75
TAED0,581,20,51-0,0150,28
Sulfonated phthalocyanine zinc0,0030 0,00120,00300,0021-
S-ACMC0,10,06---
Direct violet 9--0,00030,00050,0003-
Acid blue 29-----0,0003
Sulfate/MoistureUp to 100%Up to 100%Up to 100%Up to 100%Up to 100%Up to 100%

Examples 7-10

Table 2
Granular detergent compositions for washing, intended for automatic washing machines front load.
7 (wt.%)8 (wt.%)9 (wt.%)10 (wt.%)
Line Las87,176,5
AE3S04,805,2
Alkylsulfate1010
AE2,203,20
With10-12-dimethylhydroxylamine chloride0,750,940,980,98
Crystalline layered silicate (δ-Na2Si2O5)4,104,80
Zeolite And20017 0
Citric acid3534
Sodium carbonate15201420
Silicate 2R (SiO2:Na2O in the ratio 2:1)0,0800,110
Dirt repellent agent0,750,720,710,72
Copolymer of acrylic acid/maleic acid1,13,71,03,7
Carboxymethylcellulose0,151,40,21,4
Protease (56,00 mg active substance/g)0,370,40,40,4
Termamyl®(21,55 mg active ve is esta/g) 0,30,30,30,3
Lipex®(18,00 mg active substance/g)0,050,150,10,5
Natalase®(8,65 mg active substance/g)0,10,140,140,3
TAED3,64,03,64,0
Percarbonate1313,21313,2
Na-salt Ethylenediamine-N,N1-dinternal acid, (S,S)isomer (EDDS)0,20,20,20,2
Hydroxyethylphosphonate (HEDP)0,20,20,20,2
MgSO40,420,42 0,420,42
Flavor0,50,60,50,6
The agglomerate of antifoam0,050,10,050,1
Soap0,450,450,450,45
The sodium sulfate22332430
Sulfonated phthalocyanine zinc (active)0,00070,00120,0007-
S-ACMC0,010,01-0,01
Direct violet 9 (active)--0,00010,0001
Water and differentUp to 100% Up to 100%Up to 100%

Any of the above compositions are used for washing fabrics at a concentration of 10000 million-1in water, 20-90°C. and with a ratio of vodadone 25:1. The typical pH of approximately 10.

Examples 11-16

Liquid detergent composition for washing heavily soiled fabrics

Table 3
11 (wt.%)12 (wt.%)13 (wt.%)14 (wt.%)15 (wt.%)16 (wt.%)
AES12-15-alkylators(1.8)-sulfate111046,326,08,2
Line Las4083,34,03,0
HSAS05,130 20
Formate sodium1,60,091,20,041,61,2
Sodium hydroxide2,3the 3.81,71,92,31,7
Monoethanolamine1,41,4901,00,71,351,0
Diethylene glycol5,50,04,10,05,5004,1
Non-ionic0,40,60,30,320,3
The chelator0,150,150,110,07 0,150,11
Citric acid2,53,961,881,982,51,88
C12-14dimethylamine0,30,730,230,370,30,225
With12-18fatty acid0,81,90,60,990,80,6
Borax1,431,51,10,751,431,07
Ethanol1,541,771,150,891,541,15
Ethoxylated (EO15) Tetraethylenepentamine10,30,33 0,230,170,00,0
Ethoxylated hexamethylenediamine were20,80,810,60,40,00,0
1,2-Propandiol0,06,60,03,30,00,0
Protease*36,436,427,318,236,427,3
Mannaway®*1,11,10,80,61,10,8
Natalase®*7,37,35,53,77,35,5
Lipex®*10 3,20,53,22,43,2
Liquitint®purple ART (active)0,0060,002---0,002
S-ACMC--0,010,050,010,02
Water, flavouring, dyes and other componentsRestRestRestRestRestRest

Raw materials and notes examples of compositions 1-16

Line Las with an average length of the aliphatic carbon chain With11-C12was delivered Stepan, Northfield, Illinois, USA.

With12-14-dimethylhydroxylamine chloride was delivered Clariant GmbH, Sulzbach, Germany.

AE3S is a12-15-alkylators(3)sulfate was supplied Stepan, Northfield, Illinois, USA.

AE7 is an ethoxylate C12-15-is pirta, with an average degree of amoxilonline 7 was delivered Huntsman, Salt Lake City, Utah, USA.

Sodium tripolyphosphate was delivered Rhodia, Paris, France.

Zeolite - supplied Industrial Zeolite (UK) Ltd/, Grays, Essex, UK.

1.6 has been released silicate was delivered Coma, Nestemica, Czech Republic.

Sodium carbonate was delivered Solvay, Houston, Texas, USA.

Polyacrylate MW 4500 - was supplied by BASF, Ludwigshafen, Germany.

Carboxy methyl cellulose Finnfix®BDA was delivered CPKelco, Arnhem, Netherlands.

Savinase®, Natalase®, Lipex®, Termamyl®, Mannaway®was delivered Novozymes, Bagsvaerd, Denmark.

Fluorescent Brightener 1 is a Tinopal®AMS

fluorescent Brightener 2 is a Tinopal®CBS-X.

Sulfonated phthalocyanine zinc and direct violet 9, which isone Pergasol®violet BN-Z - all came Ciba Specialty Chemicals, Basel, Switzerland.

Diethylenetriaminepentaacetic acid was supplied by Dow Chemical, Midland, Michigan, USA.

Percarbonate sodium was delivered Solvay, Houston, Texas, USA.

Perborate of sodium was supplied by Degussa, Hanau, Germany.

NOBS - sodium nonanoyloxybenzenesulfonate was delivered Eastman, Batesville, Arkansas, USA.

TAED - tetraacetylethylenediamine were supplied under the trademark Peractive®the company Clariant GmbH, Sulzbach, Germany.

S-ACMC is a carboxymethyl cellulose, conjugate with C.I. reactive blue 19, sold by the company Megazyme, Wicklow, Ireland under the trade Zn is com AZO-CM-CELLULOSE, product code S-ACMC.

Dirt repellent agent is Repel-o-tex®PF - supplied Rhodia, Paris, France.

Copolymer of acrylic acid/maleic acid with a molecular weight of 70,000 and a ratio akilimali 70:30 was supplied by BASF, Ludwigshafen, Germany.

Protease FN3 was delivered Genencor International, PaloAlto, California, USA.

Na-salt Ethylenediamine-N,N'-dinternal acid, (S,S)isomer (EDDS) was delivered Octel, Ellesmere Port, UK.

Hydroxyethylphosphonate (HEDP) was supplied by Dow Chemical, Midland, Michigan, USA.

The agglomerate of antifoam was delivered Dow Corning, Midland, Michigan, USA.

HSAS is a branched alkylsulfate with an average chain length, as disclosed in US 6020303 and US 6060443.

With12-14-dimethylamine was delivered Procter & Gamble Chemicals, Cincinnati, Ohio, USA.

Non-ionic, preferably represents C12-C13ethoxylate, preferably with an average degree of amoxilonline 9.

The protease was delivered Genencor International, Palo Alto, California, USA.

Liquitint®purple ART - came Milliken, Spartanburg, South Carolina, USA).

* The values are expressed in mg enzyme/100 g

1as described in US 4597898.

2available under the trademark LUTENSIT®BASF and such as described in WO 01/05874.

Although there have been illustrated and described specific ways embodiment of the present invention, specialists in the art it will be obvious that various other changed who I am and modifications can be made without violating the essence and scope of the invention. Therefore, it is implied that the attached claims cover all such changes and modifications are included in the scope of the present invention.

1. A composition comprising a lipase and a colouring agent for fabric, and the lipase is a lipase having at least 90% homology with SEQ ID NO:2 and containing a substitution of an electrically neutral or negatively charged amino acid at R or at any of positions 3, 224, 229, 231 and 233, and the specified colouring agent for the fabric selected from the group consisting of dyes, conjugates dye-clay and mixtures thereof.

2. The composition according to claim 1, characterized in that the lipase is present in amount of from 0.0005% to 0.1% and the specified colouring agent to tissue is present in the amount from 0.00003% to 0.1%.

3. The composition according to claim 1, characterized in that the lipase is selected from the group consisting of lipases, classified AS, and mixtures thereof, the said dyes selected from the group consisting of dyes with small molecules, polymer dyes and their mixtures, and these conjugates dye-clay selected from the group consisting of conjugated dye-clay comprising at least one cationic/basic dye and smectite clay, and mixtures thereof.

4. The composition according to claim 2, characterized in that the lipase is selected from the group consisting of lipases with Klah is eficacia ES, and mixtures thereof, the said dyes selected from the group consisting of dyes with small molecules, polymer dyes and their mixtures, and these conjugates dye-clay selected from the group consisting of conjugated dye-clay comprising at least one cationic/basic dye and smectite clay, and mixtures thereof.

5. The composition according to claim 3, characterized in that the lipase is selected from the group consisting of lipases, classified E.C.3.1.1.3, and their mixtures, these dyes with small molecules selected from the group consisting of direct violet 9, direct violet 35, direct violet 48, direct violet 51, direct violet 66, direct blue 1, direct blue 71, direct blue 80, direct blue 279, acid red 17, acid red 73, acid red 88, acid red 150, acid violet 15, acid violet 17, acid violet 24, acid violet 43, acid violet 49, acid blue 15, acid blue 17, acid blue 25, acid blue 29, acid blue 40, acid blue 45, acid blue 75, acid blue 80, acid blue 83, acid blue 90 and acid blue 113, acid black 1, basic violet 1, basic violet 3, basic violet 4, basic violet 10, basic Phi is violet 35, basic blue 3, basic blue 16, basic blue 22, basic blue 47, basic blue 66, basic blue 75, basic blue 159, and their mixtures, these polymeric dyes selected from the group consisting of polymers containing conjugated Chromogens, polymers with Chromogens, copolymerizable in the main chain of the polymer, and mixtures thereof, these conjugates dye-clay conjugates selected from the dye-clay, comprising a dye selected from the group consisting of basic yellow C.I. 1-108, basic orange C.I. 1-69, basic red C.I. 1-118, basic violet C.I. 1-51, basic blue C.I. 1-164, basic green C.I. 1-14, basic brown C.I, 1-23, basic black C.I. 1-11, and clay selected from the group consisting of montmorillonite clay, hectorite clay, aponitolau clay and mixtures thereof.

6. The composition according to claim 5, characterized in that the dye with a small molecule selected from the group consisting of acid violet 17, acid violet 43, acid red 73, acid red 88, acid red 150, acid blue 25, acid blue 29, acid blue 45, acid blue 113, acid black 1, direct blue 1, direct blue 71, direct violet 51, and mixtures thereof.

7. The composition according to claim 6, characterized in that the dye with a small molecule selected from the group consisting of acid f is autovogue 17, direct blue 71, direct violet 51, direct blue 1, acid red 88, acid red 150, acid blue 29, acid blue 113, or mixtures thereof.

8. The composition according to claim 5, characterized in that the dye with a small molecule selected from the group consisting of acid violet 17, acid violet 43, acid red 73, acid red 88, acid red 150, acid blue 25, acid blue 29, acid blue 45, acid blue 113, acid black 1, direct blue 1, direct blue 71, direct violet 51, and mixtures thereof.

9. The composition according to claim 8, characterized in that the dye with a small molecule selected from the group consisting of acid violet 17, direct blue 71, direct violet 51, direct blue 1, acid red 88, acid red 150, acid blue 29, acid blue 113, or mixtures thereof.

10. The composition according to claim 5, wherein the lipase is a variant of SEQ ID NO:2, and the specified option contains a substitution T231R and N233R.

11. The composition according to claim 10, characterized in that the dye with a small molecule selected from the group consisting of acid violet 17, acid violet 43, acid red 73, acid red 88, acid red 150, acid blue 25, acid blue 29, acid blue 45, acid blue 113, acid what about the black 1, direct blue 1, direct blue 71, direct violet 51, and mixtures thereof.

12. The composition according to claim 8, characterized in that the dye with a small molecule selected from the group consisting of acid violet 17, direct blue 71, direct violet 51, direct blue 1, acid red 88, acid red 150, acid blue 29, acid blue 113, or mixtures thereof.

13. The composition according to claim 1, characterized in that the composition includes an auxiliary material.

14. The composition according to item 13, characterized in that the auxiliary material selected from the group consisting of sulfonated zinc phthalocyanine, sulfonated aluminum phthalocyanines, xanthene dyes, and mixtures thereof.

15. The composition according to claim 1, characterized in that the said composition is a composition for cleaning and/or processing.

16. The composition according to claim 1, characterized in that it contains a lipase and a colouring agent for fabric, and the specified colouring agent for the fabric selected from the group colouring agents for fabrics that meet the requirements of test Method 1 in section test Methods of the present description, provided that the specified colouring agent for the fabric is not ultramarine blue.

17. The composition according to claim 1, characterized in that made with the possibility to get in contact with a specified surface or tissue, and the ZAT is optional and can be removed when washing and/or rinsing with a given surface and/or fabric.



 

Same patents:

FIELD: chemistry.

SUBSTANCE: detergent contains the components as follows, wt %: anionic surface active agent (SAG) - alkylbenzol sodium sulphonate 10-16; nonionic SAG - oxyethylated fatty alcohols or oxyethylated alkylphenol 2-5; sodium tripolyphosphate 15-25; organophosphonate compound - sodium salt 1-hydroxyethylidene of phosphonic acid or sodium diethylentriaminopentaxys-(methylene phosphonate) 0.2-0.6; polycarboxylate 0.5-1.5; carboxymethyl cellulose 0.3-0.6; modified polyalkylene glycol 0.2-0.6; optical bleaching agent 0.05-0.3; soda ash 3-6; liquid glass 3.5-6.0; enzyme 0.4-0.7; defoaming agent 0.05-1.5; aromatiser 0.15-0.3; sodium sulphate and water to 100.

EFFECT: higher efficiency of bleaching detergent, all type decontamination with additional softening of fabric and without irritation of hand skin during manual washing, lower temperature modes of washing, reduced damage effect on metal parts of washing machines.

10 cl, 2 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: invention is meant for soaking, washing and bleaching all types of textile articles, except articles from natural silk and wool, in any kind of machine, and also hand wash. Substance contains in % mass: an anionic surfactant 7-15, nonionic surfactant oxyethylated fatty alcohol 2-5, sodium tripolyphosphate 15-25, sodium ethylene-diaminotetraacetate (versene) 0.3-1, polycarboxylate, containing monomers of acrylic acid 0.2-0.6, carboxymethyl cellulose (CMC) (in terms of 100% of the share of the main substance) 0.4-0.6, sodium silicate (in terms of SiO2) - 2-5, sodium perborate or sodium percarbonate (in terms of activated oxygen) 1.5-4, tetraacetylethylenediamine (TAED) 0.5-3, enzymes 0.4-1, caustic ash 5-15, mixture of sodium carbonate and amorphous sodium silicate 2-5, optical brightener 0.05-0.4, perfume 0.1-0.3, sodium sulphate and water till 100.

EFFECT: increase in the effectiveness of the detergent with a whitening effect, removal of all kinds of dirt while adding extra softness without an irritating effect on the skin during hand washing, reducing the temperature regimes of washing, reduction in the harmful effect to the metallic part of the washing machine.

9 cl, 2 tbl

FIELD: chemistry.

SUBSTANCE: invention pertains to compositions of granulated synthetic detergents, meant for manual and machine washing (in any type of machine) and soaking all types of textiles, including coloured and other household needs. The detergent contains the following components in the given % mass: anionic surface active agent sodium alkylbenzenesulfonate 9-12; non-ionic surfactant 2-5; sodium tripolyphosphate 15-30; dispergator 0.3-1.2; carboxymethyl cellulose 0.3-0.7; sodium silicate 3-6; modified polyalkylene glycol 0.2-0.6; enzyme 0.3-0.8; sodium carbonate 6-12; photo-bleaching agent 0.003-0.020; optical brightener 0.05-0.20; fragrance component 0.1-0.3; sodium sulphate and water up to 100. The non-ionic surfactant preferably contains oxyethylated monoalkylphenols based on propylene trimers or oxyethylated fatty alcohols. The dispergator used is sodium polyacrylate or sodium salt of a copolymer of maleic or acrylic acid or a sodium salt of a copolymer of acrylic, methacrylic and maleic acid. Alternatives of the synthetic detergent are also described.

EFFECT: increased effectiveness of the detergent, bleaching effect after washing, wider assortment of high quality granulated synthetic detergents.

7 cl, 1 tbl

FIELD: enzymology, protein engineering, in particular enzyme with proteolysis activity.

SUBSTANCE: invention relates to new subtilisin variants obtained by substitution in amino acid sequence of wild-type enzyme following by certain charge alteration in corresponding site, namely negative charge increasing (positive charge decreasing) or vice versa. Subtilisin variants with the first-type substitutions have higher cleaning activities mainly in systems with law detergent concentrations. Subtilisin variants with the second-type substitutions have higher cleaning activities mainly in systems with high detergent concentrations. Variants being effective both in systems with low and high detergent concentration also are disclosed. New subtilisin variants are obtained by expression of DNA mutant sequence in cells of strain Bacillus. Subtilisin muteins of present invention are useful in cleaning compositions and composition for cloth treatment, as well as in animal feed additives.

EFFECT: enzyme of improved effectiveness.

18 cl, 4 dwg, 14 tbl, 2 ex

FIELD: gene and protein engineering, in particular substances for detergents and cleaning compositions.

SUBSTANCE: invention relates to mutant forms of subtilisin Bacillus, obtained by certain combination of replacement in origin native enzyme amino acid sequence. Such mutant forms are differ from precursor subtilisin by increased cleaning effect. According to present invention all subtilisin variants are characterized either by residue substitution in position accepted to 232 position of Bacillus amyloliquifaciens amino acid sequence with valine, or by residue substitution in position accepted to 212 position of said subtilisin natural form with proline. To produce disclosed new form of enzyme respective mutant DNA sequences have been expressed in host cells, preferably in strain Bacillus cells with decreased level of proteases activity. Subtilisin variants of present invention are useful as components of any cleaning composition allowing for protease including.

EFFECT: new materials for detergents and cleaning compositions of improved effect.

6 cl, 5 dwg, 6 tbl, 3 ex

The invention relates to detergents and can be used both for manual and machine washing and soaking of all kinds of textile products, including non-ferrous, and other household needs

The invention relates to biotechnology; multicomponent system for mediated mediated enzymatic oxidation includes (a) an oxidation catalyst chosen from the group margantsovistyh oxidase, (b) an oxidizing agent chosen from the group comprising oxygen and oxygen-containing compounds, b) the mediator from the group of compounds containing Mn ions

The invention relates to the field of dyeing and finishing production and, in particular, to a method for removal of excess dye from printed or dyed fabric or yarn, comprising processing a rinsing solution containing at least one enzyme exhibiting peroxidase or LACCASO activity, in a concentration of 0.005 to 5 mg protein enzyme per 1 l of a solution for rinsing, oxidizing agent, mediator - 1-hydroxybenzotriazole in a concentration of from 1 μm to 1 mm and, optionally, additives

The invention relates to a paste-like compositions of detergent designed for washing and decontamination of clothing from various tissue and polymer materials

The invention relates to multiphase detergent tablets, and how they are used in washing machines

FIELD: gene and protein engineering, in particular substances for detergents and cleaning compositions.

SUBSTANCE: invention relates to mutant forms of subtilisin Bacillus, obtained by certain combination of replacement in origin native enzyme amino acid sequence. Such mutant forms are differ from precursor subtilisin by increased cleaning effect. According to present invention all subtilisin variants are characterized either by residue substitution in position accepted to 232 position of Bacillus amyloliquifaciens amino acid sequence with valine, or by residue substitution in position accepted to 212 position of said subtilisin natural form with proline. To produce disclosed new form of enzyme respective mutant DNA sequences have been expressed in host cells, preferably in strain Bacillus cells with decreased level of proteases activity. Subtilisin variants of present invention are useful as components of any cleaning composition allowing for protease including.

EFFECT: new materials for detergents and cleaning compositions of improved effect.

6 cl, 5 dwg, 6 tbl, 3 ex

FIELD: enzymology, protein engineering, in particular enzyme with proteolysis activity.

SUBSTANCE: invention relates to new subtilisin variants obtained by substitution in amino acid sequence of wild-type enzyme following by certain charge alteration in corresponding site, namely negative charge increasing (positive charge decreasing) or vice versa. Subtilisin variants with the first-type substitutions have higher cleaning activities mainly in systems with law detergent concentrations. Subtilisin variants with the second-type substitutions have higher cleaning activities mainly in systems with high detergent concentrations. Variants being effective both in systems with low and high detergent concentration also are disclosed. New subtilisin variants are obtained by expression of DNA mutant sequence in cells of strain Bacillus. Subtilisin muteins of present invention are useful in cleaning compositions and composition for cloth treatment, as well as in animal feed additives.

EFFECT: enzyme of improved effectiveness.

18 cl, 4 dwg, 14 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: invention pertains to compositions of granulated synthetic detergents, meant for manual and machine washing (in any type of machine) and soaking all types of textiles, including coloured and other household needs. The detergent contains the following components in the given % mass: anionic surface active agent sodium alkylbenzenesulfonate 9-12; non-ionic surfactant 2-5; sodium tripolyphosphate 15-30; dispergator 0.3-1.2; carboxymethyl cellulose 0.3-0.7; sodium silicate 3-6; modified polyalkylene glycol 0.2-0.6; enzyme 0.3-0.8; sodium carbonate 6-12; photo-bleaching agent 0.003-0.020; optical brightener 0.05-0.20; fragrance component 0.1-0.3; sodium sulphate and water up to 100. The non-ionic surfactant preferably contains oxyethylated monoalkylphenols based on propylene trimers or oxyethylated fatty alcohols. The dispergator used is sodium polyacrylate or sodium salt of a copolymer of maleic or acrylic acid or a sodium salt of a copolymer of acrylic, methacrylic and maleic acid. Alternatives of the synthetic detergent are also described.

EFFECT: increased effectiveness of the detergent, bleaching effect after washing, wider assortment of high quality granulated synthetic detergents.

7 cl, 1 tbl

FIELD: chemistry.

SUBSTANCE: invention is meant for soaking, washing and bleaching all types of textile articles, except articles from natural silk and wool, in any kind of machine, and also hand wash. Substance contains in % mass: an anionic surfactant 7-15, nonionic surfactant oxyethylated fatty alcohol 2-5, sodium tripolyphosphate 15-25, sodium ethylene-diaminotetraacetate (versene) 0.3-1, polycarboxylate, containing monomers of acrylic acid 0.2-0.6, carboxymethyl cellulose (CMC) (in terms of 100% of the share of the main substance) 0.4-0.6, sodium silicate (in terms of SiO2) - 2-5, sodium perborate or sodium percarbonate (in terms of activated oxygen) 1.5-4, tetraacetylethylenediamine (TAED) 0.5-3, enzymes 0.4-1, caustic ash 5-15, mixture of sodium carbonate and amorphous sodium silicate 2-5, optical brightener 0.05-0.4, perfume 0.1-0.3, sodium sulphate and water till 100.

EFFECT: increase in the effectiveness of the detergent with a whitening effect, removal of all kinds of dirt while adding extra softness without an irritating effect on the skin during hand washing, reducing the temperature regimes of washing, reduction in the harmful effect to the metallic part of the washing machine.

9 cl, 2 tbl

FIELD: chemistry.

SUBSTANCE: detergent contains the components as follows, wt %: anionic surface active agent (SAG) - alkylbenzol sodium sulphonate 10-16; nonionic SAG - oxyethylated fatty alcohols or oxyethylated alkylphenol 2-5; sodium tripolyphosphate 15-25; organophosphonate compound - sodium salt 1-hydroxyethylidene of phosphonic acid or sodium diethylentriaminopentaxys-(methylene phosphonate) 0.2-0.6; polycarboxylate 0.5-1.5; carboxymethyl cellulose 0.3-0.6; modified polyalkylene glycol 0.2-0.6; optical bleaching agent 0.05-0.3; soda ash 3-6; liquid glass 3.5-6.0; enzyme 0.4-0.7; defoaming agent 0.05-1.5; aromatiser 0.15-0.3; sodium sulphate and water to 100.

EFFECT: higher efficiency of bleaching detergent, all type decontamination with additional softening of fabric and without irritation of hand skin during manual washing, lower temperature modes of washing, reduced damage effect on metal parts of washing machines.

10 cl, 2 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: present invention relates to compositions which contain certain versions of lipase and a toned agent for fabric, and includes use of such compositions for cleaning and/or treating surface areas or fabric.

EFFECT: improved deposition of temporary colourant, reduced activity of the enzyme which leads to bad smell, and improved perception of cleanness.

17 cl, 3 tbl, 16 ex

FIELD: chemistry.

SUBSTANCE: compositions contain certain versions of lipase and a photo-bleaching agent based on xanthene dye, a photoinitiator and their mixtures.

EFFECT: more efficient cleaning, leading to minimal undesirable bad smells.

11 cl, 3 tbl, 16 ex

FIELD: chemistry.

SUBSTANCE: detergent composition contains a polymer combined with a surfactant or an inorganic detergent component and auxiliary ingredients. The fat purification efficiency index of the detergent composition is at least equal to 10. The polymer is a random grafted copolymer which has a hydrophilic skeleton and hydrophobic side chains, obtained by grafting (a) polyethylene oxide; (b) vinyl ester of acetic and/or propionic acid; and/or C1-4alkyl ester of acrylic or methacrylic acid; and (c) modifying monomers. The detergent compositions preferably additionally contain lipase enzyme.

EFFECT: improved removal of fat contaminants and stains with reduced amounts of conventional surfactants or inorganic detergent components.

22 cl, 6 ex

FIELD: chemistry.

SUBSTANCE: present invention relates to a composition which contains lipase, and a bleaching catalyst which can accept an oxygen atom from a peroxy acid and transfer the oxygen atom to an oxidisable substrate.

EFFECT: less bad smell which arises when using lipase enzymes in detergents.

13 cl, 4 tbl, 3 ex

FIELD: chemistry.

SUBSTANCE: cleaning compositions contain organic catalysts having improved enzyme compatibility and having the following formulae: where each R1 independently denotes a branched alkyl group selected from a group comprising 2-butyloctyl, 2-pentylnonyl, 2-hexyldecyl, isodecyl, isotridecyl and isopentadecyl, or a linear alkyl group containing 11-18 carbon atoms. The composition also contains one or more auxiliary ingredients.

EFFECT: cleaning compositions with efficient bleaching at low water temperature.

15 cl, 16 ex

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