Linking molecules having therapeutic activity

FIELD: chemistry.

SUBSTANCE: invention relates to immunology and biotechnology. Described are versions of the humanised antibody CD45RO/RB which carry a light and a heavy strand. Versions of the following are disclosed: isolated polynucleotide, coding antibody, expression vector containing a polynucleotide and host cells containing the expression vector. Described also is use of the antibody to treat and/or prevent various diseases, including as a component of a pharmaceutical composition.

EFFECT: invention provides antibodies identified as CD45RO and CD45RB, which can find use in medicine.

9 cl, 14 dwg, 2 tbl, 13 ex

 

The text descriptions are given in facsimile form.

1. Humanitariannet antibody to CD45RO/RB containing the polypeptide having the sequence of SEQ ID NO:31 or SEQ ID NO:32, and poly is epted, having the sequence of SEQ ID NO:7 or SEQ ID NO:8.

2. Humanitariannet antibody to CD45RO/RB, which contains
the polypeptide having the sequence of SEQ ID NO:31, and a polypeptide having the sequence of SEQ ID NO:7,
the polypeptide having the sequence of SEQ ID NO:31, and a polypeptide having the sequence of SEQ ID NO:8,
the polypeptide having the sequence of SEQ ID NO:32, and a polypeptide having the sequence of SEQ ID NO:7, or
the polypeptide having the sequence of SEQ ID NO:32, and a polypeptide having the sequence of SEQ ID NO:8.

3. Selected polynucleotide encoding the antibody according to one of claims 1 and 2.

4. The expression vector containing polynucleotide according to claim 3.

5. Selected eukaryotic cell host, which includes the expression system according to claim 4.

6. Application gumanitarnogo antibodies according to one of claims 1 and 2 or dedicated polynucleotide according to claim 3 for the treatment and/or prevention of autoimmune diseases, transplant rejection, psoriasis, dermatitis, inflammatory bowel diseases and/or allergies.

7. The use according to claim 6 for the treatment and/or prevention of reaction of graft-versus-host (GVHD).

8. Application gumanitarnogo antibody according to claim 6 for preparing a medicinal product intended for the treatment of transplant rejection of pancreatic islet cells.

9. Pharmaceutical the song for the treatment and/or prevention of autoimmune diseases, transplant rejection, psoriasis, dermatitis, inflammatory bowel diseases and/or allergies, comprising a therapeutically effective amount gumanitarnogo antibodies according to one of claims 1 to 3 in combination with at least one pharmaceutically acceptable carrier or diluent.



 

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FIELD: chemistry.

SUBSTANCE: invention relates to humanised anti-TGF-beta-antibody which is linked to TGF-beta. The humanised antibody has a variable domain VH which contains residues of the hypervariable region (non-human), which are contained in the human domain VH which includes a modified framework region (FR) (amino acid and nucleotide sequences are given in the list of sequences). The humanised antibody can contain residues of the complementarity determining region (CDR) of the variable domain of the light strand VL. The invention also relates to a composition for treating TGF-beta mediated disorders, e.g. malignant tumours, nucleic acid, coding monoclonal antibody, and a method of obtaining the latter using host cells. The invention provides a method of treating and detecting TGF-beta in a sample from the body using the disclosed antibody, as well as to a product which contains the humanised antibody and directions for use for treating TGF-beta mediated disorders.

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57 cl, 45 dwg, 4 tbl, 8 ex

FIELD: medicine.

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EFFECT: invention can be used in medicine and researches.

12 cl, 3 dwg, 3 tbl, 3 ex

Product and method // 2385931

FIELD: medicine.

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15 cl, 5 dwg, 1 tbl, 2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: there is described an immunogen for making an immunogenic cancer composition free of DNA-binding function and all domains of a zinc finger, on the basis of polynucleotide coding a nonfunctional mutant form of a related molecule ("brother") of regulator of imprint sites (BORIS) of protein, polypeptide or peptide, containing amino acid sequence presented in the description. The immunogenic cancer composition contains aforementioned immunogen and an adjuvant chosen particularly from cytokine, chemokin, a costimulating molecule. There is described an expression vector containing polynucleotide, coding above-stated protein, e.g., in bacterial systems, mammal systems, in yeast or viral systems. The cancer vaccine under the invention contains polynucleotide (immunogen), additionally the adjuvant and, if necessary, a pharmaceutically acceptable carrier. The invention describes the method for of cancer immunisation of a mammal with using said immunogen on the basis of polynucleotide.

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28 cl, 7 dwg, 2 tbl, 1 ex

FIELD: medicine.

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14 cl, 1 tbl, 6 ex

FIELD: medicine.

SUBSTANCE: there is offered molecule of nucleic acid inducing CEA immune response, containing a nucleotide sequence that codes a fused protein on a basis of carcinoembryonal antigen (CEA) or its functional version fused with a subunit B of thermolabile enterotoxin E coli. There are described versions thereof, as well as the related purified protein. There is disclosed an expression vector containing said molecule of nucleic acid, and a host-cell containing specified vector. There are described adenoviral vaccinal vector for inducing the immune response and a vaccinal plasmid on the basis of the specified molecule.

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20 cl, 62 dwg, 20 ex

FIELD: biotechnologies.

SUBSTANCE: invention is related to the field of biotechnology, specifically, to separation and identification of new genes of spiramycins biosynthesis track and to new polypeptides, which participate in this biosynthesis, and may be used to produce acyltransferase, which is responsible for modification of platenolid in position 3. Polynucleotide coding acyltransferase, which is responsible for modification of platenolid in position 3, cells of bacterium Streptomyces type are transformed, and strain-producer of end polypeptide is made.

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26 cl, 41 dwg, 44 tbl, 31 ex

FIELD: biotechnologies.

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EFFECT: creation of model cat for research of retroviral infection.

24 cl, 10 dwg, 6 tbl, 8 ex

FIELD: pharmacology.

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10 cl, 26 dwg, 2 tbl, 22 ex

FIELD: pharmacology.

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35 cl, 13 dwg, 36 tbl, 14 ex

FIELD: chemistry.

SUBSTANCE: invention relates to humanised anti-TGF-beta-antibody which is linked to TGF-beta. The humanised antibody has a variable domain VH which contains residues of the hypervariable region (non-human), which are contained in the human domain VH which includes a modified framework region (FR) (amino acid and nucleotide sequences are given in the list of sequences). The humanised antibody can contain residues of the complementarity determining region (CDR) of the variable domain of the light strand VL. The invention also relates to a composition for treating TGF-beta mediated disorders, e.g. malignant tumours, nucleic acid, coding monoclonal antibody, and a method of obtaining the latter using host cells. The invention provides a method of treating and detecting TGF-beta in a sample from the body using the disclosed antibody, as well as to a product which contains the humanised antibody and directions for use for treating TGF-beta mediated disorders.

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57 cl, 45 dwg, 4 tbl, 8 ex

FIELD: medicine.

SUBSTANCE: there is offered a monoclonal antibody specific to human interleukine-4 (hIL-4) containing two domains with the related CDR1-3 region. There are described versions thereof that contain specified CDR, polynucleotide coding said antibody. There are described an expression vector and a host-cell for preparing the antibody to human interleukine-4 (hIL-4). There are opened: application of the antibody for preparing a pharmaceutical agent for treating the diseases mediated by interleukine-4 and/or IgE. There is discovered the pharmaceutical composition for treating the diseases mediated by interleukine-4 and/or IgE is opened.

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14 cl, 1 tbl, 6 ex

FIELD: pharmacology.

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10 cl, 26 dwg, 2 tbl, 22 ex

FIELD: pharmacology.

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35 cl, 13 dwg, 36 tbl, 14 ex

FIELD: medicine.

SUBSTANCE: human antibodies or their antigen-binding fragments are fully described, which specifically bind to human 4-1BB, and which provide for binding of human 4-1BB to human 4-1BB ligand. Antibody includes variable areas of light and heavy chains with amino-acid sequence, given in formula, and may in one of aspects represent IgG4 antibody. Invention presents polynucleotides, which code amino-acid sequences of heavy chain and light chain of monoclonal antibody. Pharmaceutical compositions are described for cancer treatment on the basis of monoclonal antibody or its fragment and method for treatment of disease in subject, which consists in introduction of therapeutically efficient amount of antibody to this subject.

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10 cl, 24 dwg, 1 tbl, 3 ex

FIELD: chemistry.

SUBSTANCE: proposed is a recombinant single-strand trispecific antibody for treating tumours which express CEA. The said antibody consists of a series of three antibody fragments: anti-CEA-scFv, anti-CD3-scFv and VH CD28-antibody, linked by two intermediate linkers (intermediate linker Fc and intermediate linker HSA). If necessary, a c-myc-mark or (His)6-mark can be added at the C-end. Described is DNA, which codes the antibody, expression vector based on it and E.coli cell, containing the vector.

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10 cl, 21 dwg, 11 ex

FIELD: chemistry, medicine.

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FIELD: medicine.

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40 cl, 69 ex, 30 dwg, 32 tbl

FIELD: biotechnology.

SUBSTANCE: present invention relates to biotechnology and immunology. Proposed here is a polynucleotide, encoding a cyclic single-stranded tri-specific antibody. The antibody is directed against human ovarian carcinoma in vitro, has mass of approximately 84 kD and consists of three components: an antibody against human ovarian carcinoma cells, anti-CD3 antibody and anti-CD28 antibody, which are joined together by peptide interlinks such that, they form a cyclic antibody. Invented is an expression vector, containing a coding polynucleotide and versions of E.coli host cell based on the polynucleotide and expression vector.

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8 cl, 12 dwg

FIELD: biology; biotechnology.

SUBSTANCE: invention concerns biotechnology. It claims linking molecule represented by monoclonal antibody or its fragment capable of binding human NogoA polypeptide, human NogoA 623-640. Polynucleotide encoding the claimed molecule is presented. Expression vector including indicated polynucleotide is described. Host cell including indicated polynucleotide or vector is described. Pharmaceutical composition for treatment of diseases related to nerve reconstruction and containing indicated molecule is described.

EFFECT: obtainment of antibodies capable of binding NogoA 623-640.

9 cl, 1 dwg, 3 tbl, 7 ex

FIELD: medicine.

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EFFECT: intensified antineoplastic activity.

19 cl, 51 ex, 2 tbl, 35 dwg

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