Immunoreactive peptide and method of diagnosing rheumatoid arthritis using said peptide
SUBSTANCE: invention relates to peptides which originate from an antigen recognised by autoantibodies used for diagnosing rheumatoid arthritis. The peptides are filaggrin molecule fragments which contain modified residues of arginine and having amino acid sequences given in the formula of invention. The invention discloses a method of diagnosing rheumatoid arthritis by detecting autoimmune antibodies using the said peptide(s) through reaction of the latter with the blood serum of patients suffering from rheumatoid arthritis. Presence of autoimmune antibodies in the analysed sample is indicated by presence of peptide complexes formed with the antibody.
EFFECT: disclosed peptide has high specificity and sensitivity.
4 cl, 1 dwg, 3 tbl
The invention relates to peptides derived from the antigen recognized by autoantibodies. The peptides are fragments of molecules filaggrin containing modified arginine residues. The invention relates also to method of detection of autoimmune antibodies in rheumatoid arthritis by interacting with autoimmune antibodies from patients suffering from rheumatoid arthritis. Rheumatoid arthritis (RA) is one of the most common human diseases. This disease according to who suffers 1-2% of the population. In Russia officially registered more than 1 million patients with RA. RA is characterized by chronic inflammation of the joints. This leads to suffering, reduced quality of life, reduce disability.
The development of RA is characterized by an extraordinary diversity of clinical manifestations and biochemical disorders. This significantly hinders early diagnosis and, therefore, the provision of adequate therapy, which is especially effective at the onset of the disease. Modern diagnosis of RA is based on clinical manifestations of the disease in combination with laboratory determination in the patient's blood so-called rheumatoid factor (RF). However, RF is not sufficiently specific marker. Two other serological markers of RA are antiperiodic the RNA factor (APF) and anticarcinoma antibodies (AKA), recognize antigenic determinants citrullinaemia protein filaggrin. Despite the high specificity of the APF and AKA for the diagnosis of RA, these autoantibodies are not widely used in clinical laboratory practice due to technical difficulties. In recent years there has been developed enzyme-linked immunosorbent (ELISA) method for the determination of antibodies to filaggrin (AF), which has a high specificity. AF found in the blood before the onset of clinical signs of RA, which demonstrates the important role of these antibodies in the pathogenesis of this disease and the ability to conduct early diagnosis for this indicator. The basis of this technique is antigenic complex in the form of polypeptides (sections molecules filaggrin) or the protein (recombinant).
From the prior art known peptides used in the method for the diagnosis of rheumatoid arthritis and described US 2004241767, US 2004253644, US 6890720, US 7288634, US 6858438.
The invention dedicated to the creation of new antigenic complex, with greater specificity and sensitivity.
According to theory of the heterogeneity of the population AF, reflecting the natural variability of antigens to which they are produced (the presence of ACE, AKA, AF), and polymorphism molecules filaggrin we can assume that there are several carolinecaroline structural participants who s this molecule, possessing antigenic properties. Computer analysis of molecules confirmed it.
Based on predictive analysis of antigenic epitopes solid-phase method using f-moc equipment was synthesized four peptide reproducing fragments of protein molecules filaggrin: f-1, f-2, f-3, f-4.
ASSHEQAXSSAGEXHGSHHQ - f-1,
CSSHEQAXSSAGEXHGCHHQ - f-2
QSHQESAXGXSGETSGHSGS - f-3,
CSHQESAXGXSGETSCHSGS - f-4
where X is Cit,
which is immunologically reactive immunological study aimed at identifying RA, and can be used in the method for the diagnosis of RA, including the detection of autoimmune antibodies in the serum of the patient by interaction of at least one of the foregoing peptides from serum of a patient and detecting the presence of the formed complexes between the peptide and an antibody.
All the preparations of peptides corresponded to 95% purity according to HPLC and mass spectroscopy and contained 20 amicalola with the replacement of two Arg residues at Cit.
ELISA-methods of measurement of antigenic activity of the peptides against AF.
1. Immobilization of IFA tablet.
On polystyrene plates (Costar, USA) was applied solutions of peptides in 0.01 M carbonate-phosphate buffer pH 7.4 at a concentration of 1 µg/100 µl. In each well of a tablet made of 100 μl of a solution. Sorption of the peptide was carried out at 2-4°C in accordance with is their 16 hours.
At the end of the sorption solution of peptide shook and each well was made a 2% solution of bovine serum albumin (BSA) (Sigma, USA). Incubation was performed for 2 hours at 37°C.
2. Analysis and detection of immune complexes (antigen-antibody).
The investigated samples were diluted 100 times with a solution of 2% BSA and the resulting solution was introduced into the wells at 100 μl. Incubated 1.5 hours at 37°C. after incubation, the tablet was washed three times with phosphate-saline with 0.05% tween-20 (SDF) 250 ál into the hole. Then for the formation of specific immune complexes in each well was made 100 ál of solution (1:1000) conjugate IgG rabbit antibodies to human IgG with horseradish peroxidase or protein a conjugate with horseradish peroxidase in 2% BSA and incubated for 1 hour at 37°C. After incubation, the solution was shaken off and the plate was washed three times with a solution of DCF. To implement the detection of immune complexes in each well was brought to 100 μl of TMB solution with hydroperiod and incubated in the dark at room temperature for 5-15 min to develop color. The reaction was stopped by adding to each well 50 µl of 50% hydrochloric acid. The colour intensity is measured on the vertical scanning photometer at 450 nm. Positive thought of serum at which the optical density (OD) was greater than the value of 0.2.
When tested immunohistochemistry its the STV peptides f-3 and f-4 showed high sensitivity AF (table 2, 3).
|Detection of antibodies to filaggrin (peptide f-3, f-4) in the sera of patients with RA|
|The total number of||Patients with AT to f-3||Patients with AT to f-4|
|Patients of RA||78||66||73|
When comparing immunochemical properties of f-3 and f-4 between themselves and with the properties already known active cyclic peptide CCP (HQCHQESTXGRSRCGRSGS, where X-Cit) was that there are patients in whom AF is defined by one of these peptides, while others do not. The highest sensitivity has f-4 (table 3, drawing). Based on these data, created antigenic complex for detection AF from a mixture of peptides (f-3; f-4).
|The data measure the optical density of the serum samples of donors and patients with RA|
|Patients||CCP(so450 nm)||F-3(OP450 nm)||F-4 (so450)|
|19||of € 0.195||0,215||0,437||0,266||0,347|
|1||0,117||has 0.168||0,123||is 0.135||0,08|
|D2||0,122||is 0.135||amount of 0.118||0,165||0,216|
|Note: in the column "patients" index "D" is marked serum donors.|
1. The peptide having the amino acid sequence and structure chosen from:
where X is Cit, intended for the diagnosis of rheumatoid arthritis.
2. The peptide according to claim 1, immunological reactive immunological study aimed at the detection of rheumatoid arthritis.
3. A method for the diagnosis of rheumatoid arthritis, including the interaction of at least one of the peptides according to claim 1 or a mixture of peptides presented in claim 1, serum (plasma) blood of the patient and the diagnosis of rheumatoid arthritis by the presence of the formed complexes between the peptide and an antibody.
4. The method according to claim 3, in which the detection involves the use of enzyme-linked immunosorbent assay (ELISA).
SUBSTANCE: invention relates to a method of extracting benzene from mixtures with non-aromatic hydrocarbons, simultaneously obtaining distillate through extractive rectification, characterised by that the selective solvent used is in form of mixtures containing 14.7-48.5 wt % sulfolane or N-formylmorpholine and 48.5-83.3 wt % methylpyrrolidone, containing 2-3 wt % water.
EFFECT: use of given method allows for obtaining benzene, toluene and distillate containing not more than 1-1,5 vol. % benzene, which can be used as a component of motor car fuel or as raw material for pyrolysis process.
1 cl, 1 ex, 4 tbl, 2 dwg
SUBSTANCE: invention relates to a method of separating alkane and alkene fractions, possibly containing alkadiene impurities, using extraction rectification in the presence of polar extraction agent(s), wherein the basic amount of alkanes comes out in a distillate stream, and the basic amount of alkenes comes out in a strippant stream distilled from the extraction agent. The method is characterised by that before extraction rectification, the larger part of 1-alkene(s) in the alkane and alkene fractions is isomerised and/or hydroisomerised to 2-alkene(s) at temperature not above 100°C in the presence of heterogeneous catalyst(s) with activity during positional isomerisation of alkene, and possibly a small amount of polar substance which does not deactivate the catalyst(s).
EFFECT: more efficient separation of alkane and alkene mixtures through extraction rectification.
11 cl, 13 ex, 1 dwg
SUBSTANCE: method includes supply of initial mixture and dimethylsulfoxide (DMSO) as separating agent, taken in ratio 7-7.5:1 to initial mixture, into extraction rectification column (1) efficiency 50 t.t., initial mixture being supplied on 30 t.t., separating agent on 10 t.t. of column (plate numeration from top of column), phlegm number in column constitutes 1.5-2, taking of benzol in distillate and mixture benzol-perfluorobenzol (PFB) - tertiary amyl alcohol (TAA)-DMSO from column (1) cube , supply of mixture PFB-TA-DMSO on 25 t.t. of column of separating agent regeneration (2) with efficiency 50 t.t., phlegm number in column being 1-3, removal separating agent from column cube and its supply to column (1), supply of column (2) distillate, representing aseotropic mixture PFB-TAA, for separation into complex of two columns (3) and (4) with efficiency 35 t.t., with removal from column cubes of TAA and PFB, respectively, aesotropic mixture being supplied on 18 t.t. of column (3), phlegm numbers of columns (3) and (4) being equal 0.5-1.5 and 1-2 respectively, re-cycle of aseotrope PFB-TAA, which is separated in distillate of column (4) into column(3) feeding, ratio of re-cycle of column (4) and feeding of column (3) being (1-1.1):0.66, pressure in columns (1)-(3) is 300 mm of mercury, pressure in column (4) - 760 mm of mercury.
EFFECT: simplification of technology, increase of ecological compatibility of process and quality of obtained products.
1 tbl, 1 dwg, 1 ex
SUBSTANCE: described is the method of obtaining unrefined 1, 3-butadiene with the help of extractive distillation from C4-fractions, which contain C4-acetylenes as the secondary components, with the use of a selective solvent. The method is achieved in a column with dividing partitions, which contains in the bottom part an evaporator, in which lengthwise there is a dividing partition, which forms the first zone, the second zone and the underlying combined zone of the column, connected along the upper flow with the extractive washing column. Supply of energy to the column with the dividing partition through the lower evaporator is regulated such that from the column with the dividing partition draw off the lower stream, which contains the solvent, saturated with C4-acetylenes, in which the portion of 1, 3-butadiene is limited with the estimation that the 1, 3-butadiene lost is economically acceptable. In this case the lower stream is submitted into the decontaminator for acetylenes, from which C4-acetylenes are removed and the purified solvent is removed from it from the lower stream.
EFFECT: increase in the periods of the operation of the device between the cleaning cycles.
11 cl, 1 tbl, 1dwg, 1ex
SUBSTANCE: method of separation of starting mixture (A) consisting of two or more constituents, by extractive distillation with the selective solvent (S) within dividing wall column (TKW), is proposed. The separation is performed in the dividing wall column (TKW) having a dividing wall aligned in a longitudinal direction (TW) and extending to an upper end of the column and dividing an interior of the column into first region (1), second region (2), and lower combined column region (3). The starting mixture is fed into first region (1), first top stream (B) is taken off from first region (1), and second top stream (C) is taken off from second region (2), with each of the streams having a prescribed specification. The selective solvent (S) is introduced in an upper part of first region (1) and/or in an upper part of second region (2), and flow of solvent (S1) into the first region (1) and/or flow of solvent (S2) into second region (2) are set so that each of the prescribed specifications for top streams (B, C) are met.
EFFECT: invented method of dividing mixtures is more efficient in terms of energy and solvent consumption.
6 cl, 7 dwg, 1 tbl
FIELD: petrochemical processes.
SUBSTANCE: invention relates to a method for continuously separating C4-fraction by extractive distillation using selective solvent on extractive distillation column, which method is characterized by a separation barrier disposed in extractive distillation column in longitudinal direction extending to the very top of the column to form first zone, second zone, and underlying common zone. Butanes (C4H10)-containing top stream is withdrawn from the first zone, butenes (C4H8)-containing top stream is withdrawn from the second zone, and C4H6 stream containing C4-fraction hydrocarbons, which are more soluble in selective solvent than butanes and butenes, is withdrawn from underlying common zone of column.
EFFECT: reduced power consumption and expenses.
15 cl, 2 dwg, 2 ex
FIELD: petrochemical processes.
SUBSTANCE: hydrocarbon mixture obtained by extractive distillation of C4-fraction using selective solvent, which mixture contains those C4-hydrocarbons, which are better soluble in selective solvent than butanes and butenes, is subjected to continuous separation. Mixture is supplied to first distillation column, wherein it is separated into top stream, containing 1,3-butadiene, propine, and, if necessary, other low-boiling components and, if necessary, water, and bottom stream containing 1,3-butadiene, 1,2-butadiene, acetylenes, and, if necessary, other high-boiling components. Proportion of 1,3-butadiene in bottom stream of the first distillation column is controlled in such a way as to be high enough to dilute acetylenes beyond the range wherein acetylenes can spontaneously decompose. Top stream from the first distillation column is passed to second distillation column, wherein it is separated into top stream, containing propine, and, if necessary, other low-boiling components and, if necessary, water, and bottom stream containing pure 1,3-butadiene.
EFFECT: simplified process and reduced power consumption.
FIELD: petroleum processing and petrochemistry.
SUBSTANCE: in particular, invention aims at producing extraction dearomatized component from reformat of gasoline fraction, which component may be used in production of petroleum solvents such as hexane solvents. Process comprising countercurrent extraction of aromatic hydrocarbons with liquid selective extractant to separate dearomatized component (raffinate) and subsequent extractive rectification of resulting extract phase by distilling off aromatic hydrocarbons is characterized by that liquid selective extractant is diethylene glycol or triethylene glycol, countercurrent extraction is carried out at 125-140°C, extractive rectification is carried out using process steam in presence of saturated selective extractant wherein evaporation of water is performed with the aid of energetic steam, unsaturated selective extractant after extractive rectification and recycled gasoline are sent to extraction stage preliminarily using unsaturated selective extractant as heat carrier to generate process steam, and energetic steam condensate is used to heat recycled gasoline to 80-130°C.
EFFECT: enhanced process efficiency.
3 cl, 1 dwg, 1 tbl, 3 ex
FIELD: petroleum processing.
SUBSTANCE: separation of crude C4-fraction comprises rectification of C4-fraction containing butanes, butenes, 1,3-butadiene, and small amounts of other hydrocarbons, including C4-acetylenes, 1,2-butadiene, and C5-hydrocarbons, via extractive distillation using selective solvent. Crude C4-fraction is fed to middle part of first extractive distillation column and selective solvent is added to column above crude C4-fraction introduction point. Vaporous side stream containing C4-acetylenes together with 1,3-butadiene1,2-butadiene, C5-hydrocarbons, and selective solvent, wherein concentration of C4-acetylenes is below self-decomposition threshold, is withdrawn from the first distillation column from the point below crude C4-fraction introduction point. Top stream containing crude C4-fraction components, which are less soluble in selective solvent than C4-acetylenes, are withdrawn from upper part of the first extractive distillation column.
EFFECT: optimized order of process operations.
21 cl, 1 dwg
FIELD: industrial organic synthesis.
SUBSTANCE: crude 1,3-butadiene is recovered from C4-fraction by extractive distillation using selective solvent on column separated by a partition installed along longitudinal direction of the column to form first and second subzones and underlying common column zone. The column is connected to preswitched flush column. Distillation column operation is controlled by energy supply with the aid of lower evaporator and distribution of a series of theoretical plates within underlying common zone to create bottom stream therefrom consisting of purified solvent.
EFFECT: simplified process technology.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to medical technologies, immunology, pharmacology and particularly to drugs for treating the patients with rheumatoid arthritis (RA) and to methods of treating thereof to be used in rheumatology. Substance of the invention includes application of Fc-fragments of immunoglobulin class G as an antigen for treating RA, a remedy for treating RA, produced by mixing the antigen and an adjuvant, and the related method of treating RA.
EFFECT: improved effect to suppress autoimmune response.
6 cl, 3 ex, 4 dwg
SUBSTANCE: group of inventions refers to medicine and pharmacology. There is offered therapeutic agent for osteoarthritis treatment, containing hyaluronane containing 2 to 20 sugar links as an operating component. Preferentially, hyaluronane has the form of tetrasaccharide consisting of two links with a single link in the form of: -D-glucuronic acid-b-1,3-D-N-acetylglycosamine-b-1,4-(HA4). There is offered method of osteoarthritis treatment that involves the stage of introduction of effective amount of hyaluronane containing 2 to 20 sugar links to a subject requiring such treatment.
EFFECT: invention presents a new drug, effective for osteoarthritis treatment with the minimum undesired reaction.
6 cl, 1 ex, 2 tbl
SUBSTANCE: invention relates to a group of novel chemical compounds pharmacologically acceptable salts thereof having formula , where A represents COOH; B represents H; n equals 0; V represents -CH2-, a single bond; W represents a 5-7-member heteroaromatic group with one heteroatom selected from N, O, S which can optionally be substituted with 1-3 substitutes selected from a group of substitutes A, when V represents a -CH2-group, where if V represents a single bond, W represents a bicyclic condensed a ring -member heterocyclic group with one heteroatom selected from O, S, which can optionally be substituted with 1-3 substitutes selected from a group of substitutes A; X represents a 5-7-member heteroaromatic group with one O atom and one or two N atoms, which can optionally be substituted with 1-3 substitutes selected from a group of substitutes A; Y represents C6-C10 aryl which can optionally be substituted with 1-3 substitutes selected from a group of substitutes A, a 5-7-member heteroatomatic group with one S atom which can optionally be substituted with 1-3 substitutes selected from a group of substitutes A; Z represents C1-C8 alkyl, C3-C7 cycloalkyl which can optionally be substituted with 1-5 substitutes selected from a group of substitutes A; C6-C10 aryl which can optionally be substituted with 1-5 substitutes selected from a group of substitutes A; C6-C10 aryloxy which can optionally be substituted with 1-5 substitutes selected from a group of substitutes A, or C1-C12 aralkyl which can optionally be substituted with 1-5 substitutes selected from a group of substitutes A; group of substitutes A represents halogen, C1-C6 alkyl, halogen C1-C6 alkyl, C1-C6 alkoxy.
EFFECT: compounds exhibit inhibitory activity towards HvGR which enables their use to prepare a pharmaceutical composition used in therapy for autoimmune diseases.
33 cl, 6 tbl, 30 ex
SUBSTANCE: invention relates to novel compounds of formula , where Qa is phenyl or heteroaryl, and Qa can possibly carry 1 or 2 substitutes selected from hydroxy, halogen, amino, (1-6C)alkyl, (1-6C)alkoxy, (1-6C)alkylamino and di-[(1-6C)alkyl]amino; R1 and R2 are each independently selected from hydrogen and (1-6C)alkyl; Qb is phenyl or heteroaryl, and Qb can possibly carry 1 or 2 substitutes selected from hydroxy, halogen, (1-6C)alkyl, (3-6C)cycloalkyl, (1-6C)alkoxy, (1-6C)alkoxycarbonyl, amino, (1-6C)alkylamino, di-[(1-6C)alkyl]amino, hydroxy-(1-6C)alkyl, (1-6C)alkoxy-(1-6C)alkyl, amino-(1-6C)alkyl, (1-6C)alkylamino-(1-6C)alkyl, (1-6C)alkylthio, (1-6C)alkylsulfinyl and (1-6C)alkylsulfonyl; where any of the substitutes Qa and Qb defined above, containing a CH2 group which is bonded to 2 carbon atoms, or a CH3 group bonded to a carbon atom, can possibly carry on each of the said CH2 or CH3 group one or more substitutes selected from hydroxy, amino, (1-6C)alkyl, (1-6C)alkoxy, (1-6C)alkylamino and di-[(1-6C)alkyl]amino; where heteroaryl is an aromatic 5- or 6-member monocyclic ring which can contain up to three heteroatoms selected from oxygen, nitrogen and sulphur, and can be condensed with a benzene ring or a five-member nitrogen-containing ring containing 2 nitrogen atoms; as well as pharmaceutically acceptable salts thereof. The invention also relates to a method of producing formula I compounds, a pharmaceutical composition and use of these compounds for treating conditions mediated by effect of TNF cytokines.
EFFECT: more effective treatment.
13 cl, 3 tbl, 46 ex
SUBSTANCE: combined chondroprotective pharmaceutical composition is made in the form of gel and contains chondroitin sodium sulphate, glucosamine sulphate sodium chloride, propylene glycol, Carbomer (Carbopol), lavender oil, methyl parahydroxybenzoate (Nipagin, methylparaben), sodium methabisulphite, ethanol and purified water in the relevant ratio of the components.
EFFECT: stimulated mechanisms of cartilage reparation, suppressed activity of enzymes destroying cartilage tissue, anaesthetising and antiinflammatory action within an application area.
SUBSTANCE: invention refers to compounds of formula I or formula II, to their pharmaceutically acceptable salts, enantiomers and diastereoisomers as metalloprotease inhibitors, and also to a pharmaceutical composition based thereon and to versions of application thereof. Said compounds can find application in treatment of the diseases mediated by activity of metalloproteases, Her-2 SHEDDASE, ADAM-10 and ADAM-17, such as arthritis, cancer, cardiovascular disorders, skin diseases, inflammatory and allergic conditions, etc. In general formula I or II: A represents CWNHOH; B represents CH2; G represents CH2; D represents oxygen; X represents CH2NRb; Y represents CH2; M represents C; U is absent or represents NRb; V is absent or represents phenyl, or 4-10-members heterocyclyl containing 1-2 heteroatoms chosen from N and S, substituted with 0-5 groups Re; U' is absent or represents C1-10alkylene, O or combinations thereof; V' represents H, C1-8alkyl, NRbRc, C6-10carbocyclyl substituted with 0-3 groups Re, or 5-14-members heterocyclyl containing 1-3 heteroatoms chosen from N, O and C substituted with 0-4 groups Re; Ra and Re, independently represents H, T, C1-8alkylene-T, C(O)NRa'(CRb'Rc')r-T, (CRb'Rc')r-O-(CRb'Rc')r-T, OH, Cl, F, CN, NO2, NRIRII, COORIV, ORIV, CONRIRII, C1-8halogenalkyl, C3-13carbocyclyl; Rb and Rc independently represents H, T, C1-6alkylene-T, C(O)O(CRb'Rc')r-T, C(O)(CRb'Rc')r-T, S(O)p(CRb'Rc')r-T; T represents H, C1-10alkyl substituted with 0-1 groups Rb'; C3-6carbocyclyl, 5-6-members heterocyclyl containing one oxygen atom; Ra' Rb' and Rc' independently represents H, ORIV or phenyl; R1 represents hydrogen; R2 represents hydrogen; R3 represents: (i) C1-10alkyl; (ii) 4-14-members heterocyclyl containing 1-3 nitrogen atoms optionally substituted with one or two substitutes chosen from C1-6alkyl, OR13, 5-10-members heterocyclyl containing 1-3 heteroatoms chosen from N O and C, or phenyl; (iii) NR16R17; R4 represents H; R4' represents H; R5' represents H; W represents oxygen; R13 represents C1-C6alkyl; R16 and R17 independently represents C1-C10alkyl or phenyl where each is optionally substituted with one C1-4alkyl; RI and RIIindependently represents H or C1-6alkyl; RIV represents C1-6alkyl; i is equal to 0; p is equal to 1 or 2 and r is equal to 0, 1 or 2; provided that a) a spiro ring represents a stable chemical base unit and b) NR8 and NRb do not contain neither N-N, nor N-O bonds.
EFFECT: higher efficiency of the composition and method of treatment.
54 cl, 1 tbl, 9 dwg, 284 ex
SUBSTANCE: invention refers to chemical-pharmaceutical industry, and concerns an external preparation used for treating arthropathies and soft tissue conditions (osteoarthroses, osteochondroses, tendovaginites, bursites, ligamentites, etc.) based on a complexon of diphosphonic acid derivatives with carbohydrate amines expressing chondroprotective, calcium regulating and anti-inflammatory activity. A preparation for treating arthropathies and soft tissue conditions based on diphosphonic acid derivatives contains as an active component the complexonate of Na, K salt of 1-hydrohy-ethylenediphosphonic acid and glycosamine, adjuvants and water, and represented in the form of gel or cream.
EFFECT: invention allows reducing or eliminating joint synovitis and oedema, downsising or softening ossificates in soft tissues, considerably lowering or arresting painful symptom, as well as reducing density and size of hematomas.
16 ex, 4 dwg, 4 cl
SUBSTANCE: invention refers to medicine, namely to physiotherapy. Therapeutic technique involves heating of applicators containing pre-homogenised Tambukan mud to 36-38°C. The homogenised mud contains (wt %): crystal skeleton 15.0÷25.0, colloid complex 10.0÷20.0, the rest - mud solution. The heated applicators are placed on a projection of a diseased organ. Then electrodes are placed on applicators with a cathode on one, and an anode on the other. Direct current of density 0.03-0.07 mA/cm2 is supplied on the electrodes. In the first procedure the DC supply time is 10-20 minutes. In each following procedure, the DC supply time is two minutes more than in the previous one.
EFFECT: method improves clinical effectiveness ensured by higher activity of biological components of the mud.
7 cl, 4 ex
SUBSTANCE: invention refers to medicine, namely to orthopaedics and neurology, and can be used in treating the patients suffering from vertebral osteochondrosis. That is ensured by plasmapheresis with sampling blood in amount 800-900 ml. Thrombocytes are removed with following elimination of plasma in amount 450-500 ml. The prepared plasma is injected intramuscularly paravertebrally in four points two from each side in projection of 3-5 lumbar vertebra 2-3 cm away from spinous process at a depth 3-4 cm in amount 15 ml for each point. Two days later plasma is introduced again in the same regimen. Therapeutic course is carried out to 2-4 times every 4-5 days.
EFFECT: method allows improving clinical effectiveness in the patients suffering from said pathology due to normalisation of coagulative blood system, microcirculation, metabolic processes, liquidation of kallikrein-kinin system hyperactivity and tissues oedema owing to removal of thrombocytes, and due to local prolonged action of autoplasma on various links of disease pathogenesis.
SUBSTANCE: invention refers to medicine, namely to physiotherapy. An enclosed tampon applicator is filled with homogenised native mud of Tambukan lake containing (wt %): crystal skeleton 15.0÷25.0, colloid complex 10.0÷20.0 and mud solution - the rest. Then the tampon applicator is placed in a light- and gas-tight enclosure and heated to 36 - 40°C. Then the applicator is pulled out of the light- and gas-tight enclosure. The heated applicator is placed on projection of a human organ, then covered with a hot-water bottle pre-heated to temperature at least 50°C. The procedure takes 30 minutes and lower.
EFFECT: method improves regenerative clinical effectiveness ensured maximum preservation of therapeutic properties of the prepared medical mud.
9 cl, 2 ex
SUBSTANCE: invention is related medicine and concerns applications of antibodies specifically recognising any prevailing variants of beta-amyloid peptide, Aβ40 and Aβ42, in preparation of a drug applied for prevention and-or treatment of Alzheimer's disease.
EFFECT: invention provides prevention of progression or reduction of symptoms, and/or decrease in amyloid deposition in an individual when administering an immunostimulating dose of peptide or specific antibody.
7 cl, 3 ex, 2 dwg