Method for predicting depressive behaviour in aged primate, rates of aging and development of stress-dependent pathological changes |
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IPC classes for russian patent Method for predicting depressive behaviour in aged primate, rates of aging and development of stress-dependent pathological changes (RU 2377570):
Method of estimating efficiency of neoadjuvant autohemochemotherapy in patients suffering from carcinoma of cervival stump / 2375063
Invention concerns medicine, namely oncology and can be used in estimation of clinical effectiveness factors in treatment of carcinoma of cervical stump. Daily urine is analysed for estradiol content and if decreased after treatment from initial level within 8.44-12.85 nmol/days, positive antineoplastic effect of treatment is stated, and if urine estradiol content is maintained in the treated patients within 8.81-11.38 nmol/days, the absence of antineoplastic effect is stated. The obtained results allow considering dynamics of estradiol content in daily urine of the patients suffering from carcinoma of cervical stump prior to AHCT as an additional laboratory test to estimate efficiency of presented pharmacotherapy. Observed positive clinical effect is confirmed by higher operability and improved life quality of the patients.
Method of determining effeciency of larynx cancer treatment / 2375003
Invention relates to medicine, namely to oncology and can be applied in presence of locally advanced process. In daily urine of patients before treatment tetrahydro-11-desoxycortisol is determined. If its level before treatment does not exceed 2 mcmol/day relapse-free period is forecasted during 2 and more years; initial level of fraction over 2 mcmol/day indicates about disease relapse or metastases during first year after treatment.
Way of forecasting of restoration of menstrual function at patients with nervous anorexia / 2363002
Invention concerns medicine, namely to gynecology, and can be used at treatment of patients with amenorrhea caused by nervous anorexia. Level of leptin is defined in blood serum using enzyme immunoassay method for realisation of the way of forecasting of restoration of menstrual function at patients with a nervous anorexia. Then an index of mass of a body (BMI) and a parity leptin/BMI is counted. In case the parity leptin/BMI makes 0.8 and more - normalisation of menstrual function is prognosticated.
Way of diagnostics of male sterility / 2362167
Invention concerns medicine area, namely to andrology, and concerns diagnostics of male sterility. The way includes studying compaction degree of chromatin of spermatozoids, percentage of defective cells in an ejaculate, level of B in blood serum. On the basis of the received data diagnostic index DI is calculated; its value helps to diagnose sterility, or absence of pathology.
 Method of diagnosing functional hyperprolactinaemia in girls / 2351935
In girls with suspected functional hyperprolactinaemia determined are level of prolactin two hours after falling asleep, concentration of general triiodtrionin, cortisol, and ultra-sound examination of small pelvis organs is carried out in order to determine volume of right ovary. On the basis of obtained data canonical value (K) is calculated by formula, and if K is greater than (-2.28) and lower than 1.27 functional hyperprolactinaemia is determined.
 Method of diagnosing hormonally inactive microadenomas of hypophysis in girls / 2351934
Invention can be used for diagnosing hormonally inactive microadenomas of hypophysis (IAH) in girls. In order to realise the method levels of prolactin in blood is determined in girls with suspected two hours after falling asleep and at 6 o'clock in the morning. On the basis of obtained data, canonical value (K) is calculated by formula, and if K is greater than (-1.69) and smaller than 1.86 IAH is diagnosed.
Method of determination of orientation of pathological process at cancer of ovaries / 2350966
Invention concerns medicine, namely to onco-gynecology. The essence of the method consists in the following: level of Testosteron is defined and at its content peer or above 2.5 nmol/l, intraoperative in blood of women with tumorous formations of ovaries before operation. In a tissue of a tumour of ovaries the maintenance of Testosteron is defined and at augmentation of its indicator above 2 nmol/g operation of full volume is carried out.
 Evaluation method for perioperative stress and quality of organism nociceptive protection / 2342669
Invention relates to medicine, namely surgery and anesthesiology, and refers to evaluation method for perioperative stress and quality of organism nociceptive protection. The method consists in radioimmunoassay of cortisol content in daily urine before and after the operation, beginning with first post-operative urine portion, and finishing with next day morning urine. If cortisol concentration has grown twice and higher, then inadequate organism protection from operative stress and post-operative pain syndrome is stated.
 Method of predicting of life expectancy of patients with local cancer of prostate / 2341197
Define risk factors from the anamnesis of a life and clinico-laboratory indicators: the age, degree of differentiation of a tumour, level of Testosteron-Depotum and prostates-specific antigen in blood serum (PSA), carry on prediction depending on their values, thus in addition define time from the development of hormone-resistance beginning, estimate level of painful sensations and the general condition of the patient on scale ECOG, define degree of disturbance of an emiction on international IPSS scale, establish gradation and numerical value of risk factors then define life expectancy of the patient in months under the formula: Y=-9.17+0.47•X1+1.94•X2-0.698•X3+0.01•X4 2+0.07•X3 2-1.66•X5 2+1.86•X6 2-0.0003•X7 2+0.01•X8 2, where: Y - life expectancy of the patient in months; X1, 2...8 - gradation and numerical value of risk factors, and X1 - age, years; X2 - tumour differentiation: G0 - low-grade tumour =0, G1 differentiated tumour =1, G2 - moderate-grade tumour =2, G3 - a low-grade tumour =3, G4 - an undifferentiated tumour =4; X3 - Testosteron-Depotum level in ng/ml; X4 - time from the beginning of hormone-resistance development in months; X5 - level of painful sensations of the patient: absence of pain =0, mild pain =1, moderate pain d-2, strong pain =3, intolerable pain =4; X6 - general condition of the patient on ECOG scale: activity of the patient is unlimited =0, restriction by kinds of activity of demanding efforts =1, walking and capable itself to serve, but incapable to perform any work =2, is limited capable for Self-Care =3, completely incapable of any activity =4; X7 - level of the PSA in ng/ml; X8 - degree of disturbance of an emiction at patients on international scale IPSS (from 0 to 36 points).
Method of diagnostics of vibration sickness degree / 2337363
Invention concerns medicine and can be used for definition of degree of expression of vibration sickness. For realisation of the method perform determination of blood rheumatic factor and antibodies to cardiolipin, phagocytic activity of blood, level of CD-4+- and CD-16 of +-lymphocytes, M immunoglobulin (Ig M) and cytokine IL-10 (IL-10), adrenocorticotropic and thyritropic hormones ("АКТГ" and "ТТГ") in the blood. Define prognostic factors F1 and F2 and compare them on the basis of the obtained data. In case if F1 it is higher than F2, diagnose vibration sickness of I degree, and if F1 is less than F2 - vibratory illness of II degree.
Method for differential diagnostics of mammary diseases in men / 2244308
The present innovation deals with biochemical trials: before the onset of therapy in men one should detect blood content of thyroid hormone - free thyroxine - and at its level being 10.3-12.9 pmol/l one should diagnose mammary cancer, at the level of free throxine being 18.7-31.0 pmol/l - one should predict gynecomastia. The method enables to detect the direction of pathological process and carry out due correction of therapy tactics in men with either gynecomastia or mammary cancer.
Method for predicting the delay of intrauterine fetal development / 2246733
The method deals with studying blood serum of pregnant woman to detect the content of insulin-like growth factor (IGF) and vascular-endothelial growth factor (VEGF) and calculate the coefficient of their ratio: at its value being equal to 28.5 and lower it is possible to diagnose the delay of fetal development.
Method for differential diagnostic of chronic hepatitis and hepatic cirrhosis / 2254577
Thyroglobulin content is determined in blood serum using enzyme immunoassay. When thyroglobulin level is increased by 2 times and more compared in contrast with normal one chronic hepatitis is diagnosed, and when thyroglobulin level is decreased by 1.5-2.5 times in contrast with normal one hepatic cirrhosis is diagnosed.
Method for evaluating combined schizophrenia treatment based on atypical antipsychotic drugs and rations and electroconvulsive shock therapy / 2256181
Method involves determining dehydroepiandrosterone sulfate concentration in blood serum. Its growth above 30% when compared to the initial one being observed, treatment efficiency is determined as negative.
Method for diagnosing obliterated forms of congenital suprarenal gland cortex hyperplasia / 2261447
Method involves applying high effectiveness liquid chromatography for determining cortisol, cortisone 11-deoxycorticosterone, 11-deoxycortisone concentration in blood and free cortisol and free cortisone excretion with urine. Ratios of F/E and FF/FE are calculated, where F is the cortisol level in blood; E is the cortisone level in blood; FF is the free cortisol excretion with urine and FE is the free cortisone excretion with urine. The cortisol level in blood not exceeding norm and at least two of three signs: F/E ratio reduction by 25% and more, FF/FE ratio reduction by 25% and more, free cortisol excretion with urine being equal to 25% and more, obliterated forms of congenital suprarenal gland cortex hyperplasia is diagnosed. Corticosterone level in blood growing by 50% and higher, 21-hydroxylase defect is considered to be the case. 11-deoxycorticosterone and/or 11-deoxycortisone concentration in blood being et or greater than 50%, 11β-hydroxylase defect is considered to be the case.
 Method for diagnosing atrophic gastritis cases / 2262706
Method involves making pepsinogen 1, gastrin and Helicopter pylori infection marker combinations analysis and making input of the obtained results into data processing means comprising operation system, means for receiving, transmitting and processing data. The mentioned data processing means is usable for comparing the measured concentration value of a substance under study to a threshold value associated to the substance under study and producing information as a response to comparison results and additionally to other entered data. A set and software are used for implementing the method.
Method for predicting relapse of mammary cancer / 2263319
In the course of surveying in menopausal women after complex therapy one should state the development of mammary cancer at decreased ratio of estriol concentration to the sum of estrone and estradiol urinary concentrations from 1.68±0.23 in relapse-free patients up to 0.74±0.12 - in patients living without relapses for less than a year, up to 0.65±0.13 in patients living without relapse from 2 up to 6 years and up to 0.50±0.10 in patients with relapse-free period from 6 to 10 years. The innovation provides pre-clinical detection of mammary cancer relapse.
Method for predicting fetoplacental insufficiency in pregnant women with thyroid diseases / 2263919
Except detecting placental lactogen in blood serum one should study the content of alphafetoprotein. At placental lactogen content being below 75% against the norm and content of alphafetoprotein below 70% against the norm it is possible to conclude upon availability of fetoplacental insufficiency.
Method for predicting cholecystitis and cholelithiasis / 2263920
While diagnosing cholecystitis and cholelithiasis due to ultrasound testing one should additionally study blood plasma and bile to detect there the content of prostaglandins PGE2 and PGF2α. At PGE2/PGF2α ratio in blood plasma being equal to 6 and more, and, also, at decreased level of biliary cholecystokinin-pancreosimin by 38% and more, biliary PGE2 by 59% and more and increased level of biliary prostaglandin PGF2α by 5.9 times and more against the norm one should diagnose chlecystitis and cholelithiasis. The innovation enables to detect the above-mentioned diseases at earlier stage.
Method for predicting powerless labor in pregnant nodular goiter surgically treated on goiter occasion during pregnancy under medical supervision / 2273456
Method involves determining thyroid gland node diameter and thyrotropic hormone by applying ultrasonic examination approach. Histological examination is carried out with conclusion concerning morphologic nature being obtained like nodular colloid proliferating goiter or thyroid gland adenoma. Diagnostic index Σ is calculated from formula Σ=0.49*K1+0.07*K2-0.5*K3+1.76*K4-1.53, where K1 is the thyroid gland node diameter; K2 is the TTH concentration; K3 is the nodular colloid proliferating goiter index equal to 1 or 0; K4 is the thyroid gland adenoma index equal to 1 or 0. Diagnostic index Σ being less than zero, conclusions concerning powerless labor threat is to be drawn.
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FIELD: medicine. SUBSTANCE: invention refers to medicine and veterinary science and can be used for predicting depressive behaviour of an aged primate, rates of ageing and development of stress-dependent pathological changes. According to the claimed invention, an aged primate is examined for psychoemotional reaction on soft and acute psychoemotional stress effect, molar concentration ratio of cortisol and dehydroepiandrosterone sulphate in peripheral blood plasma and percentage concentration of cortisol in peripheral blood plasma in the evening (20 o'clock 45 minutes - 21 o'clock 15 minutes) to concentration of cortisol in peripheral blood plasma in the morning (8 o'clock 45 minutes - 9 o'clock 15 minutes) to predict depressive behaviour in the aged primate with accelerated rates of ageing and development of stress-dependent pathological changes in depressive reaction on soft and acute psychoemotional stress effect, if molar concentration ratio of cortisol and dehydroepiandrosterone sulphate in peripheral blood plasma is not less than 4.3, and concentration of cortisol in peripheral blood plasma in the evening to concentration of cortisol in peripheral blood plasma in the morning is not less than 66%. EFFECT: ensured information value of predicting depressive behaviour in the aged primate, rates of ageing and development of stress-dependent pathological changes. 3 ex
The invention relates to medicine and veterinary medicine and can be used to predict depressive behavior of an old Primate, the rate of aging and the development of stress-related pathological changes. Known dependence mediated by fear of the startle reflex (for example, blink reflex) from the level of cortisol and dehydroepiandrosterone sulfate in the blood. In the study, respondents with a high ratio of cortisol/dehydroepiandrosterone sulfate reflex blinking was stronger pronounced than respondents with a low ratio of cortisol/dehydroepiandrosterone sulfate, which confirmed the stress-buffer effect of dehydroepiandrosterone sulfate (Grillon C, Pine D.S., Baas J.M., Lawley M, Ellis V, Charney D.S. Cortisol and DHEA-S are associated with startle potentiation during aversive conditioning in humans // Psychopharmacology. - 2006. - Vol.186, N 3. - P.434-441). It is known that with increasing age, the molar ratio of cortisol/DHEA sulfate. This occurs by reducing the level of secretion of dehydroepiandrosterone sulfate. Hyposecretion of dehydroepiandrosterone sulfate is the reason for the decline in dehydroepiandrosterone sulfate - mediated antiglucocorticoid activity that increases the neurotoxicity of glucocorticoids. In the result of age-related hormonal changes are developing neurodegenerative PA who alogia (E. Ferrari, L. Cravello, Muzzoni Century, Casarotti D., Paltro M., Solerte S.B., Fioravanti M., Cuzzoni G., Pontiggia Century, Magri F. Age-related changes of the hypothalamic-pituitary-adrenal axis: pathophysiological correlates // European journal of endocrinology. - 2001. - Vol.144, No. 4. - 319-329). It is established that the ratio of cortisol/DHEA sulfate was significantly higher in the group of hospitalized older people (Carvalhaes-Neto N., Huayllas, M.K., Ramos LR, Cendoroglo M.S., Kater C.E. Cortisol, DHEAS and aging: resistance to Cortisol suppression in frail institutionalized elderly // European journal of endocrinology. - 2003. - Vol.26, No. 1. - P.17-22). The main disadvantage of this research is that they aren't helpful when predicting depressive behavior of an old Primate, the rate of aging and the development of stress-dependent pathological changes due to the absence of comprehensive studies of psychological and endocrinological parameters. The basis of the invention is to provide informative predict depressive behavior of an old Primate, the rate of aging and the development of stress-related pathological changes. The problem is solved by the fact that the old primacy determine emotional responses to mild and acute psycho-emotional stress exposure, the ratio of the molar concentrations in peripheral plasma cortisol and dehydroepiandrosterone sulfate and the percentage share in the concentration of cortisol in plasma ne is fericelli blood night (20 hours 45 minutes 21 hours 15 minutes) relative to the concentration of cortisol in peripheral plasma in the morning (8 hours 45 minutes 9 hours and 15 minutes) and predict depressive behavior of an old Primate, accelerated rate of aging and the development of stress-related pathological changes in depressive reactions to mild and acute psycho-emotional stress exposure, with a ratio of molar concentrations in peripheral plasma cortisol and dehydroepiandrosterone sulfate average of at least 4.3, and the percentage concentration of cortisol in peripheral plasma in the evening in relation to the concentration of cortisol in plasma, peripheral blood in the morning, amounting to at least 66%. In the first studies we have conducted, based on selecting the most reliable emotional and endocrine indicators, it was suggested that an informative combination of psychological and endocrinological parameters: the ratio of the molar concentrations in peripheral plasma cortisol and dehydroepiandrosterone sulfate, the concentration of cortisol in plasma given time interval and determining emotional reactions to mild and acute psycho-emotional stress exposure. This combination reduces the probability of accidental deviations of individual scores from the x values in aged primates with adequate behavior, with the normal rate of aging and the development of stress-related pathological changes. The inventive method of predicting depressive behavior of an old Primate, the rate of aging and the development of stress-dependent pathological changes is new and are not described in literature. The technical result of the claimed invention is the provision of informative predict depressive behavior of an old Primate, the rate of aging and the development of stress-dependent pathological changes due to the use of psychological and endocrinological parameters: the ratio of the molar concentrations in peripheral plasma cortisol and dehydroepiandrosterone sulfate, the concentration of cortisol in plasma given time interval and emotional reactions to mild and acute psycho-emotional stress exposure. The invention is illustrated in the following examples, showing the provision of informative predict depressive behavior of an old Primate, the rate of aging and the development of stress-related pathological changes. Example 1. Conducted an investigation of the old females of the species rhesus at the age of 18 years. As a mild and acute psycho-emotional stress used the changing environmental situation of the I. Determined emotional response of the animal to changing environmental conditions and the ratio of molar concentrations in peripheral plasma cortisol and dehydroepiandrosterone sulfate and the percentage share in the concentration of cortisol in plasma, peripheral blood night (20 hours 45 minutes) relative to the concentration of cortisol in plasma, peripheral blood in the morning (8 hours 45 minutes). The following results were obtained: the ratio of the molar concentrations in peripheral plasma cortisol and dehydroepiandrosterone sulfate was 4.3; the percentage concentration of cortisol in plasma, peripheral blood night (20 hours 45 minutes) relative to the concentration of cortisol in plasma, peripheral blood in the morning (8 hours 45 minutes) was 66%. As a result of the research predicted depressive behavior of an old Primate, accelerated rate of aging and the development of stress-related pathological changes. This was confirmed in long-term dynamic examination of the observed animal development depressive behavior, in 20 years the reduction in the efficiency of the production of conditioned reflexes, memory decline, increased sensitivity to external disturbances in psychological tests, the appearance of disorders of glucose tolerance, neurodegenerative changes and the infringement of the lipid metabolism. Example 2. Conducting a survey of the old male of the species baboon galadril at the age of 18 years. As a mild and acute psycho-emotional stress exposure was conducted procedural manipulation (taking blood from a finger). Determined emotional response of the animal to the taking of blood from a finger and the ratio of molar concentrations in peripheral plasma cortisol and dehydroepiandrosterone sulfate and the percentage share in the concentration of cortisol in plasma, peripheral blood night (20 hours 45 minutes) relative to the concentration of cortisol in plasma, peripheral blood in the morning (8 hours 45 minutes). The following results were obtained: the ratio of the molar concentrations in peripheral plasma cortisol and dehydroepiandrosterone sulfate was 5,75; the percentage concentration of cortisol in plasma, peripheral blood night (20 hours 45 minutes) relative to the concentration of cortisol in plasma, peripheral blood in the morning (8 hours 45 minutes) was 85%. As a result of the studies predict depressive behavior of an old Primate, accelerated rate of aging and the development of stress-related pathological changes. This was confirmed in long-term dynamic examination of the observed animal development depressive behavior, 21 year decrease in the efficiency of the criminal reflexes, memory decline, increased sensitivity to external disturbances in psychological tests, the appearance of disorders of glucose tolerance, neurodegenerative changes and disorders of lipid metabolism. Example 3. Conducted an investigation of the old females of the species rhesus at the age of 18 years. As a mild and acute psycho-emotional stress exposure was conducted with a soft immobilization of the animal. Defined emotional reaction of the animal on a soft immobilization of the animal and the ratio of molar concentrations in peripheral plasma cortisol and dehydroepiandrosterone sulfate and the percentage share in the concentration of cortisol in plasma, peripheral blood night (20 hours 45 minutes) relative to the concentration of cortisol in plasma, peripheral blood in the morning (8 hours 45 minutes). The following results were obtained: the ratio of the molar concentrations in peripheral plasma cortisol and dehydroepiandrosterone sulfate amounted to 7.2; the percentage concentration of cortisol in plasma, peripheral blood night (20 hours 45 minutes) relative to the concentration of cortisol in plasma, peripheral blood in the morning (8 hours 45 minutes) was 104%. As a result of the studies predict depressive behavior of an old Primate, accelerated the pace of ageing and development p is SS-dependent pathological changes in 22 years. This was confirmed in long-term dynamic examination of the observed animal development depressive behavior, reducing the efficiency of the production of conditioned reflexes, memory decline, increased sensitivity to external disturbances in psychological tests, the appearance of disorders of glucose tolerance, neurodegenerative changes and disorders of lipid metabolism. A method for predicting depressive behavior of an old Primate, the rate of aging and the development of stress-dependent pathological changes based on the definition of emotional and endocrine indicators, characterized by the fact that the old primacy determine emotional responses to mild and acute psycho-emotional stress exposure, the ratio of the molar concentrations in peripheral plasma cortisol and dehydroepiandrosterone sulfate and the percentage share in the concentration of cortisol in plasma, peripheral blood night (20 h 45 min - 21 h 15 min) in relation to the concentration of cortisol in plasma, peripheral blood in the morning (8 h 45 min - 9 h 15 min), - and predict depressive behavior of an old Primate, accelerated rate of aging and the development of stress-related pathological changes in depressive reactions to mild and acute psycho-emotional stress near the step, when the ratio of the molar concentrations in peripheral plasma cortisol and dehydroepiandrosterone sulfate average of at least 4.3, and the percentage concentration of cortisol in peripheral plasma in the evening in relation to the concentration of cortisol in plasma, peripheral blood in the morning, amounting to at least 66%.
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