Method for correction of age-specific and pathological human skin changes
SUBSTANCE: invention refers to medicine, namely to dermatology, and can be used for treatment or rejuvenation of human skin. It is ensured by introduction of autogenous fibroblasts suspended in physiologic saline and hyaluronic acid in treatment or rejuvenation region. The skin is prepared with growth fibroblast medium with biologically active substances (BAS) - growth medium factors including fibroblast growth in the form of gel.
EFFECT: invention provides smoothing of wrinkles, higher skin elasticity.
2 ex, 2 tbl, 52 cl
The invention relates to medicine, namely to dermatology and cell therapy, rejuvenation and treatment of human skin.
The method comprises the introduction into the area of treatment or rejuvenation of tissues or organs of a suspension of autologous (same organism) fibroblasts in physiological solution with addition of hyaluronic acid.
In addition, it is proposed to stimulate proliferative and synthetic activity of the injected fibroblasts using products fibroblast growth contained in the environment, which is used for their growth. This serves to distinguish these factors from the environment and use them as components in cosmetic tool, which then can be used before cell therapy for skin preparation, and after therapy to increase the effect of the methodology and its prolongation.
Currently, cellular therapy is carried out as follows. The syringe is injected cells in place requiring correction and then expect results. When this is used as autologous and allogeneic cells (U.S. Patent 5660850 26.08.1997; Boss W.K. Jr, Usal H, Fodor P.B, Chemoff G. Autologous cultured fibroblasts: a protein repair system. Ann Plast Surg. 2000 May 44(55): 536-542).
A similar description of the methodology is provided in another patent (Patent No. 2281776 2.08.2006), which is based on the fact that to increase the efficiency of the cell is therapy are only fibroblasts with high efficiency cloning.
Despite the increasingly widespread method is still an open question about the effectiveness of autologous cells for cell therapy, especially in adults and older people (Kregel EA, Zgurski A.A., Terekhov S.M. the Use of autologous fibroblasts in cosmetology. Aesthetic medicine. 2004. V.3. No. 4. S-342). The problem is that not always take root cells and they are not always effectively correct wrinkles or other defects.
The proposed method can significantly improve cell survival and increase the efficiency of cell therapy.
Hyaluronic acid acts as a dermal filler matrix, creating a vital volume of injected autologous cells, increases the retention of the cells prior to their introduction into the area of treatment and better survival rate of the cells after their introduction. Input solutions products is carried out with a syringe in the field of therapy. The concentration of drugs and their actions selected individually in accordance with the specific conditions and depending on the patient's age, physical condition, specific organ and tissue.
Previously, experiments were carried out on animals. For example, investigated the effects of skin fibroblasts in conjunction with the preparation of stabilized hyaluronic acid (Restylane) the devil is musnah mice (E.S. Yoon et al. Advantages of the presence of living dermal fibroblasts within restylane for soft tissue augmentation // Ann Plast Surg. 2003 Dec; 51(6). P. 587-592). The authors evaluated the possibility of increasing the validity of Restylane in combination with dermal fibroblasts. After subcutaneous injection to mice mixture 5x105fibroblasts suspended in 200 µl of phosphate-saline buffer (FSB) in combination with 200 ál of Restylane, weight of nodules formed was slightly decreased during the first 2 weeks and then did not change up to 16 weeks. The control nodules formed after injection of Restylane, diluted FSB, decreased during the observation period, 2 times. Immunochemical staining showed the presence of human collagen bundles of the experimental group compared with the control group. The obtained results indicate that Restylane, mixed with cultured fibroblasts of human skin, can be successfully injected as a living graft with the possibility of long-term effect.
We have conducted studies in which skin fibroblasts people was injected into the problem areas of the skin of the patient in a physiological solution and also together with hyaluronic acid. The ratio was as follows. Take 0.5 ml of suspensie fibroblasts with 1 million cells in physiological solution there was added 0.5 ml of hyaluronate acid. Received 0.5 million cells/ml. The original solution of hyaluronic acid which you was in the range of 0,02-0,1%, so that the final concentration in the suspension was 0.01 to 0.05%. The suspension was stirred and held around the syringe problem areas.
The results were as follows. Papules injection of fibroblasts with hyaluronic acid-kept 2 times longer than without it. This means that the injected fibroblasts have more opportunities to survive and perform their inherent functions - production of collagen and elastin, which naturally leads to wrinkles and actually seems to reduce their depth, softening the skin, improving its elasticity and firmness. Visually it is observed to reduce the visibility of wrinkles, smoothing wrinkles, evening out skin surface. The speed reduction tucks is increased at least 1.5 times that talks about real skin rejuvenation is also equivalent value.
The obtained results indicate that hyaluronic acid mixed with cultured fibroblasts of human skin, can be successfully injected as a living graft with the possibility of long-term effect.
The second proposal is to stimulate proliferative and synthetic activity of the injected fibroblasts. For this purpose it is proposed to use the products fibroblast growth contained in the environment, which is used for their growth.
Usually in cosmetic products is used substances of vegetable origin or synthesized chemically, also used hyaluronic acid (Patent RF № 94040904 from 1994.08.19).
During culturing in growth medium skin fibroblasts synthesize and secrete into the environment a large number of biologically active substances, among which are the various growth factors (EGF, FGF, TGF, KGF), components of the extracellular matrix (GAG, hyaluronic acid, chondroitin sulfate) and enzymes (EGF - like growth factor epidermis, regulates the migration and proliferation of epidermal skin cells. FGF - fibroblast growth factor stimulates the growth of all types of cells and production of extracellular matrix, TGF - trasformarsi growth factor, is involved in the formation of new blood vessels; KGF - keratinocyte growth factor (Krikheli E.A., Zgurski A.A., Terekhov S.M. the Use of autologous fibroblasts in cosmetology. Aesthetic medicine. 2004. V.3. No. 4. S-342). Synthesized also collagen and elastin components of the extracellular matrix.
The authors concluded that this environment can be used for the preparation of cosmetic products, which can be applied both before and after the application of cell therapy. This cosmetic product can be called individual (personal), because it uses the factors of the original owned by the recipient.
The presence of skin growth factors and other listed substances from the body's own cells Bud is t to contribute to the improvement of its condition to ensure the survival of existing and introduced from outside, resulting in cell therapy, cells.
On the basis of these factors serves to produce individual cosmetics under the General title: medical individual cosmetic product (MIX).
Its composition may be as follows. Lyophilized growth factors and protein ingredients of the growth medium, which increased autologous fibroblasts of the patient, glycerin, as a filler, hyaluronic acid to create a matrix in the dermis, the aromatic components to give an attractive smell.
Following the recipe.
1. Cells are grown 14-28 days on a medium containing serum, and serum-free environment.
The compounding gel, option 1:
|Growth serum-free medium with BAS||49%|
|Hyaluronic acid (sodium hyaluronate)||0,5-1,5%;|
|Glycerin (as a base)||a 49.5-48.5 per cent;|
The compounding gel, option 2:
|Growth medium with zaworotko is, containing BAS||49%|
|Hyaluronic acid (sodium hyaluronate)||0,5-1,5%;|
|Glycerin (as a base)||a 49.5-48.5 per cent;|
2. Biologically active substances produced by cells in the environment are concentrated using techniques "Minhan", "Pillicon" -"Millipore" USA or are subjected to lyophilization. The content of biologically active substances (BAS), including vitamins and amino acids growth medium, growth factors, and others, is of the order of 10 mg/ml in the growth medium.
The compounding gel, option 3:
|BAS (concentrate or freeze-dried)||0.5 to 3%|
|Glycerin (as a base)||70%;|
|Collagen of cattle||1,5%;|
|Hyaluronic acid (sodium hyaluronate)||1,5%;|
|Purified water||the required quantity to 100%.|
The term storage is of 50-60 days at +4°C.
The rate of application of 10 ampoules of 2 ml for 20 days through the day.
After 5-7 days the second course of 10 vials in a day - the minimum rate.
Courses can be applied both before and after cell therapy. Cosmetics applied as follows. Opened 1 ampoule and its volume is applied in the evening to problem areas. After that the product is not washed away until the next morning. After a day on the following night, use the second ampoule. In the following days similar uses 3-I and then the other of the ampoule 10. After cell therapy after 10 days also used other 10 ampoules. After 5-7 days the second course of 10 vials every other day.
Thus, you have the following options for the use of the individual cell cosmetics:
1) 1st year to cellular therapy;
2) 2nd year after cell therapy;
3) 2-year and 2nd year after cell therapy.
Of course, there remains the possibility of using cellular technology without the use of MIX.
MIX is a cosmetic product for external use.
Due to these factors and after their application on the skin, intercellular substance will gradually be updated and, hence, to rejuvenate.
It also creates favorable conditions for engraftment of autologous fibroblasts and their better performance in the future.
Trials were conducted at dobrovo izah. The results of the tests described below and in table 1 and confirm the industrial applicability of the method.
|The results of therapy with autologous fibroblasts without hyaluronic acid and together with the last|
|Options||Parameter estimation to therapy||Therapy:|
|fibroblasts||fibroblasts + hyaluronic acid|
|Volunteers||BOK, 52 g||DSC, 54 g|
|Note. Wrinkles, points 10-1 (depth). Turgor, points 1-10. The color of the skin in the area of the wrinkles, the score 10-0 (dark - light). Subjective assessment of 1-10 points.|
Volunteer B. O.K. 53,, female [Control]. When viewed from the skin of the face detected: decreased skin turgor in the cheeks, chin, deep wrinkles in the area of nosegun the x folds, on the nose, forehead, around the eyes; dehydration and change the contour of the face.
Diagnosis: age-related changes of the skin.
Correction of problem areas of the skin was carried out declared by way of using autologous cells and hyaluronic acid.
Purpose: transplantation of autologous fibroblasts in the problem areas of the skin together with hyaluronic acid.
To do this, the volunteer took a piece of skin from the inner surface of the forearm. Biopsy (4 mm diameter) after washing (2 times) in a solution of nutrient medium, DMEM with antibiotics penicillin (50 µg/ml) and streptomycin (50 u/ml) was placed in a Petri dish, and crushed with a scalpel in 0.1 ml of 0.25% solution of collagenase type II. After grinding the pieces of cloth covered the cover glass and the Cup was filled with 2 ml of the same solution of collagenase. The Petri dish was placed in CO2-incubator. After 1.5 h, the Cup was removed, the collagenase solution was poured off, washed with medium, DMEM with 20% fetal calf serum (ETS) and the newly poured growth environment. After 1 day, the medium was changed for fresh. After 5 days the skin pieces were washed away, with pieces of skin were placed in a centrifuge tube, centrifuged 3 min at 1000 rpm, adosados poured, the precipitate was placed in a new Petri dish was closed cover glass, poured the environment DMEM with 20% ETS and put in C02-incubator. Per adicheskii after 3 days the medium was changed for fresh. Within 15 days after reaching monolayer pieces of skin with the medium were placed in a centrifuge tube and perform similar actions. Then in a Petri dish 2 times washed the cells with EDTA, and then filled with 1 ml of trypsin solution. The Petri dish was placed in CO2-incubator. After 15 min when cells were ukreplenii from the growth surface in a bowl filled with 2 ml of growth medium, the cells are suspended and transferred into a culture flask (75 cm2and added to 10 ml of growth medium with 20% ETS, the vial was placed in CO2-incubator. In 7 days after reaching the monolayer of cells was removed from the surface of the growth in the same way and moved the cage into the vial (150 cm2. During the month of cells were perseval in a similar way to achieve monolayer in 10 vials (150 cm2. Next, cells 7 vials were removed in a similar manner, the suspension was centrifuged 2 times in saline and prepared suspension in physiological solution with a concentration of 2 million cells/ml and poured into vials, 1,5 ml After that produced the input of the suspension in the region of wrinkles insulin syringe. Before therapy, the skin surface was treated with a solution of 70% ethanol or 0.5% chlorhexidine, then did the local anesthesia 5% cream EMLA. Enter the suspension produced in the surface or medium from the second dermis 0.05 ml with an interval of 5-6 or 8 to 10 mm depending on the kind of wrinkles. Did 2 courses with an interval in 2 weeks
The surface appearance of the skin was recorded in photographs and visual observations and subjective evaluation of the patient before and after therapy.
Volunteer DSC 55,, female [Experience]. Had similar signs of skin aging with volunteer B.
Volunteer performed the same actions as the volunteer B. the Difference was that he was administered a suspension of autologous fibroblasts together with hyaluronic acid.
For this likewise were preparing a suspension of autologous fibroblasts in saline solution at a concentration of 2 million cells/ml and poured into vials, 1,5 ml After that, immediately before the procedure to cell suspension 0.5 ml) was added 0.5 ml of hyaluronic acid (sodium hyaluronate) and produced the input of the suspension in the region of wrinkles insulin syringe. Also did 2 courses with an interval in 2 weeks
The surface appearance of the skin was recorded in photographs and visual observations and subjective evaluation of the patient before and after therapy.
The results of therapy without hyaluronic acid (Volunteer B. O. K.). 4-5 months after transplantation of fibroblasts was observed smoothing of wrinkles and large creases in the forehead; smoothing wrinkles on the nose and in the lateral infraorbital region of the eyes; decreased the severity and depth of the nasolabial folds; significantly improved skin turgor, uchilsa contour and complexion.
The results of therapy with hyaluronic acid (Volunteer DSC). 4-5 months after transplantation of fibroblasts the effect is the same as the volunteer B, however, the degree of wrinkles is higher by 2-3 points on 10-point scale, the degree of patient's satisfaction above.
Similar results were obtained for another 10 patients, 5 of whom therapy without hyaluronic acid, and 5 with hyaluronic acid. Picked up a pair of volunteers so that their parameters were approximately equal.
Also, tests were carried out on volunteers individual cosmetics MIX.
The results are described below and summarized in table 2.
The volunteers carried out the same steps described above, i.e. in the same way received culture of autologous cells and also prepared cell suspension. The difference was that part of the volunteers were introduced only a suspension of fibroblasts, and the other was conducted, except of course of cell therapy, courses own cosmetics before and after application of cell therapy.
For this likewise were preparing a suspension of autologous fibroblasts in saline solution at a concentration of 2 million cells/ml and poured into vials, 1,5 ml After that produced the input of the suspension in the region of wrinkles insulin syringe 2 courses with an interval in 2 weeks.
The surface appearance of the skin was recorded in photographs, is also on visual observations and subjective evaluation of the patient before and after therapy.
Part of the volunteers did a skin treatment accessorizes MIX to cell therapy and after. This was prepared individual cosmetics, as described above.
Cosmetics MIX was used according to the following scheme:
1) 1st year to cellular therapy (10 vials every other day);
2) 2nd year after cell therapy (2 courses of 10 vials every other day).
|The results of therapy with autologous fibroblasts without individual cosmetics MIX, and together with the last|
|Patient||The estimated Parameters||The status parameter Estimation to therapy||Therapy:|
|fibroblasts||fibroblasts + MIX|
|Volunteers||BOK, 52 g||DSC, 54 g|
After applying the MIX observed the following effects compared to control.
Volunteer A.S., male, 54, skin alive, has been the absorption of seals formed after cell therapy, smoothed skin, fine wrinkles become less noticeable.
Volunteer F.A., female, 40 years: the skin has become softer, firmer, fresher, wrinkles smoothed out.
Volunteers FO, female, 45 years, LE, female, 53,, O.E., male, 43, was encountered similar changes.
In all cases there were no negative effects of the MIX, both objective and subektivniy.
Thus, the technique has proven its effectiveness and lack of allergies the x etc. negative reactions.
1. The way of rejuvenation and treatment of human skin, including the use of autologous fibroblasts with the addition of hyaluronic acid, wherein the autologous fibroblasts injected into the treatment or rejuvenation in physiological solution, while the skin is pre-treated with growth medium of fibroblasts with biologically active substances (BAS) - factors in the growth environment, including including the fibroblast growth factors.
2. The method according to claim 1, characterized in that prepare 0.5 ml of a suspension containing 1 million fibroblasts in physiological solution with the addition of 0.5 ml of a solution of hyaluronic acid at a concentration of 0,02-0,1%, the suspension is stirred and 0.1-0.2 ml injected by syringe into the area rejuvenation or treatment.
3. The method according to claim 1, characterized in that use growth environment in the composition of the gel containing fibroblasts within 14-28 days in serum-free medium, containing BAS 49%, hyaluronic acid or sodium hyaluronate 0.5 to 1.5%, glycerol is 49.5 48.5%, and flavoring 0-0,5%.
4. The method according to claim 1, characterized in that use growth environment in the composition of the gel containing fibroblasts within 14-28 days with serum content of BAS 49%, hyaluronic acid or sodium hyaluronate 0.5 to 1.5%, glycerol is 49.5 48.5%, and flavoring 0-0,5%.
5. The method according to claim 1, otlichuy is the, what factors growth medium before use concentrated using known methods or subjected to lyophilization.
6. The method according to 5, characterized in that the factors of growth medium is added to the gel in 0.5-3%, the gel also contains glycerin 70%, collagen cattle of 1.5%, hyaluronic acid and 1.5%, purified water required amount to 100%.
SUBSTANCE: invention refers to pharmacology. An antioxidant remedy is a dense extract of hop leaves (Humulus Lupulus L.).
EFFECT: application of the invention extends scope of use of herbal remedies as an antioxidant for prevention and treatment of free radical pathologies.
14 dwg, 1 tbl
SUBSTANCE: invention concerns pharmaceutical industry, particularly composition for prevention or treatment of pathological processes and maintenance of high functional activity of organism. The preventive composition applied for prevention or treatment of pathological processes and maintenance of high functional activity of organism contains combined one or more herbal extracts containing phenolic substances encapsulated in a phospholipid coating containing extracts. The coating is distributed in hydrophilic polymer, containing water 20-80% and a substance that enables dissolution of the active component in a polymer matrix, chosen of the group: ethanol, dimethyl sulphoxide, glycerine or its monoacyl derivative with certain size of particles of the composition components.
EFFECT: said composition is effective in prevention or treatment of pathological processes and maintenance of high functional activity of organism; the composition gradually releases the liposome active substance and thereby enabling permanent concentration of the active substance in organism.
2 cl, 1dwg, 2 tbl, 2 ex
SUBSTANCE: invention refers to medical products and concerns applications of a film for internal application containing triprolidine or its salt, or hydrate, as an active component of the remedy for awakening vigorous after sleeping. There is also disclosed method for treatment or prevention of sluggishness or somnolence in awakening after sleeping, the method that makes it possible for an individual to wake up vigorous after sleeping and the method ensuring falling asleep of an individual.
EFFECT: invention aims at development of the agent that makes it possible for an individual to wake up vigorous after sleeping.
19 cl, 5 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely to therapy, and can be used for treating consequences of microstroke. The method involves sequential introduction throughout the day of medical products in a single dose: mildronat, pyracetam, picamilonum and vinpocetine. Said introduction is performed during 15 days. Then these preparations are introduced in 1/4 single dose simultaneously 4 times a day during 15 days also.
EFFECT: invention use allows improving clinical effectiveness ensured by various action of preparations and reduction of their by-effects with said introduction schedule.
SUBSTANCE: invention concerns chemical-pharmaceutical industry, and can find application in manufacturing of medical, preventive, cosmetic and sanitary-and-hygienic agents like gels, sprays, balms, creams, lotions and liquids for wipes of disinfecting and sanitation actions with antifungal, antiinfective and antiviral activity without by-effects. An effective fungicidal, antiinfective, viricidal, adaptogenic and antiradiation agent contains the active substances made of vegetable raw bee balm in the form of oily, water-alcohol extracts and bee balm essence.
EFFECT: bee balm extract complex (BBEC) as a stabilised suspension of phospholipid vesicles allow incorporating lipophilic and hydrophilic fractions of complex active substances of bee balm as one structure, provides their stable transport and possesses expressed inactivating activity in relation to pathogenic microorganisms (viruses, bacteria, fungi and protozoa).
4 cl, 4 ex, 1 tbl, 1 dwg.
SUBSTANCE: invention relates to veterinary, relates to medicine, which stimulates protective powers of organism, and method of secondary immunodeficiency correction with its application. Method includes injection of non-specific immunoglobulin and additional taking of alcohol tincture of equal quantities of herb and inflorescence of Echinacea purpurea, herb of Harmal common, flowers of small-leaved lime and roots of Liquorice in form of water solution 7-8% concentration in doze 1.5-2.5 ml/kg of live weight of animal during 15 days with 24 hour interval. Medicine impacts beneficially on all organs and systems of animal organism, regulates metabolism and increases resistance of organism (activation of B- and T-systems, phagocytosis), which provides correction of secondary immunodeficiencies to level of physiological norm.
EFFECT: natural origin, possibility to metabolise easily and be excreted from organism, high compatibility with other medicines, absence of sensitisation and induction of immuno-pathological reactions.
2 cl, 6 tbl, 4 ex
SUBSTANCE: invention concerns medicine, particularly endocrinology and anesthesiology, and can be applied in pre-operation preparation of pheochromocytoma cases. It involves peroral administration of doxazozin drug in 14-21 day before operation. Initial daily drug dosage is 4 mg, by 2 mg twice per day, with further dosage increase by 2-4 mg till arterial pressure is normalised. Maximum dosage is 16 mg per day. After arterial pressure stabilisation daily doxazozin administration continues in determined therapeutic dosage. Last doxazozin take is performed in 90 minutes before narcosis in dosage of 8-12 mg.
EFFECT: prevented hypertensive crisis caused by hypercatecholaminemia during the whole operation due to timely maintenance of selective al-adrenoreceptor blockade.
1 tbl, 1 ex
SUBSTANCE: invention concerns medicine, particularly infectious diseases, and can be applied in yersiniosis infection treatment for children. It involves etiotropic and nosotropic therapy. Additionally vobenzyme is prescribed simultaneously in amount of 1 pill per 5-6 kg of body weight per day in three takes for 3 weeks.
EFFECT: enhanced efficiency, reduced therapy duration and amount of prescribed medicines, prevented development of chronic and complicated course of disease due to activation of cell-mediated immune response, controlled state of microbiocenosis in colonic mucosa.
4 ex, 2 tbl
SUBSTANCE: invention concerns medicine, particularly granulocytopoiesis stimulants, radioprotectors and immune stimulants. Under cytostatics effect Cyclophilin A amplifies granulocyte precursor removal from marrowbone and stimulates granulocytopoiesis.
EFFECT: in case of sublethal radiation, cyclophilin A stimulates migration of marrowbone stem elements and participates in recovery of blood cells and immune system, and shows redioprotection properties.
4 dwg, 2 tbl
SUBSTANCE: invention refers to medicine, radiology and ophthalmology, and can be used for reducing the risk of radiation cataract genesis at chronic effect of low doses of ionisation radiation. For that purpose water with decreased content of deuterium is injected to the organism. At that, deuterium concentration can be 1 ppm to 140 ppm. Potable water with decreased deuterium concentration can be injected to the organism orally, parenterally or by installing into the eye independently or as the solvent for physiologically active substances and pharmacological preparations. Potable water with decreased deuterium concentration can be injected to the organism till chronic influence of low doses of ionisation radiation takes place, as well as during the effect of ionisation radiation and after it.
EFFECT: method allows restraining formation of phacoscotasmus, having excluded such side effects as surface keratitis, blepharitis, and pruritus.
4 cl, 2 tbl
SUBSTANCE: invention relates to biotechnology, particularly to genetic engineering and it can be used in the biomedical industry to produce active medications of interleukin-29 (IL-29). Mutant forms of IL-29 (SEQ ID NO: 27, 29, 40, 41, 149 and 159) were offered with substitution of the cysteine residue in the position in accordance with position 171 of the aminoacid sequence of a mature protein of a wild type that are characterised by a correct formation of intramolecular disulfide bonds and accordingly they provide production of polypeptides with an antiviral activity as homogeneous medications at expression in the heterologous system.
EFFECT: vector structures and the host cells transformed by these structures for the expression of new versions of IL-29 are described.
15 cl, 37 tbl, 45 ex
SUBSTANCE: invention refers to medicine and namely gastroenterology and surgery and deals with preventive treatment and treatment of suppurative complications at acute destructive pancreatitis. For that purpose against complex conservative drug therapy there performed is sanation diagnostic abdominoscopy, and polyethylene cannula is installed in the round hepatic ligament. 70-80 ml of leukosuspension is obtained from the patient's blood on the 1st, 3rd, and 5th days of the post-operation period by means of plasmapheresis, which is then incubated with Ronkoleukin in quantity of 500000 units. Leukosuspension incubation lasts for 60 minutes at the temperature of 37°C. Then leukosuspension with Ronkoleukin is injected through the above cannula to the round hepatic ligament.
EFFECT: method provides effective treatment of acute destructive pancreatitis and namely owing to increase of local immunity, and as the result, quick normalisation of clinic laboratory values.
SUBSTANCE: invention relates to field of medicine, namely to fields of surgery and resuscitation science and can be used in treatment of patients with acute destructive appendicitis, confirmed intra-operatively. Draining of right iliac fossa or tamponade and draining of right iliac fossa are performed and anti-bacterial therapy is carried out. In case of performing appendectomy by traditional or laparoscopic method, polyethylene catheter is installed in retroperitoneal space into right iliac fossa, through which additionally introduced is roncoleukin, incubated during 60 minutes at temperature 37°C with obtained by plasmapheresis patient's leukosuspension in amount 70-80 ml on 1,3,5 day of post-operation period.
EFFECT: method allows to increase local immunity, realise prophylaxis of suppurative complications in case of acute destructive appendicitis.
SUBSTANCE: invention concerns medicine, in particular, to gastroenterology, and concerns treatment of chronic virus hepatitis B. For this purpose Roncoleukin is intravenously driply administered in a dose of 500 mg, with rate of 1-2 ml/min, during 4-6 h 2 times a week - 1 month. Then the specified preparation is administered subcutaneously, 1 ml 2 times a week within 2 months.
EFFECT: elimination of a cellular link of immunity imbalance, rapid normalisation of clinico-laboratory indicators without by-effects presentation.
SUBSTANCE: invention concerns medicine, namely odontology and can be used for treatment of pyoinflammatory diseases of soft tissues of maxillofacial area. The way is carried out as follows. Roncoleukin is administered in the exudative-inflammatory phase of a purulent wound process in the form of a solution with 500000 ME dose of active substance, into a wound preliminary irrigated by means of 10.0 ml of sterile 0.9% sodium chloride, on the turundas, dilluted either with 0.9%, or 10% sodium chloride solution. Thus a bandage from 20% dimexid solution is imposed on periphery of a purulent wound, spending daily dressings. In a proliferative phase - an irrigation of a wound using the Roncoleukin solution is combined with 500000 ME dose of active substance, diluted with 0.9% solution of sodium chloride and salve dressings with preparations on a water-soluble basis; dressings are spent in a day. The way allows normalising indicators of local immunity of a purulent wound and an oral cavity, to strengthen functional activity of phagocytes of a wound, to accelerate purification of wounds from necrotic masses, to optimise terms of occurrence of granulations.
EFFECT: possibility to normalise indicators of local immunity of a purulent wound and an oral cavity, to strengthen functional activity of phagocytes of a wound, to accelerate purification of wounds from necrotic masses, to optimise terms of occurrence of granulations.
3 tbl, 3 ex
SUBSTANCE: invention concerns medicine and can be used for treatment of damages of a GI tract mucosa. The invention represents a composition on the basis of interleukin-1 and polyvinylpyrolidone in a water containing solution, which in addition contains sodium bicarbonate, and composition components are in a certain mass parity.
EFFECT: provision of massive wound-healthing action, stimulation of local immunity of mucosa without system action.
5 ex, 6 tbl, 4 cl
FIELD: medicine, otolaryngology.
SUBSTANCE: before operation levels IL-lβ and IL-8 are determined in middle ear cavity. If value IL-lβ is higher than 323.2 pkg/ml or IL-8 is higher than 51.3 pkg/ml after operation betaleukin in form of ear drops in concentration 5 ng/ml is introduced into acoustic meatus in dose 2-3 drops daily during 7 days.
EFFECT: reduction of terms of neotympanic flap engraftment, elimination of post-operative complications.
5 tbl, 4 ex
FIELD: medicine; gastroenterology.
SUBSTANCE: invention relates to method of treating mucosal (M) lesions of gastroenterological tract (GIT). For this purpose water or physiological solution of interleukin -1 (IL-1), containing 5×10-7-5×10-5 wt % of IL-1 is introduced perorally.
EFFECT: getting high medicinal concentrations of preparation directly in lesion focus, stimulation of local immunity with no side effects.
6 cl, 6 tbl, 4 ex
SUBSTANCE: medication for treatment and prevention of asthma contains FGF2 (fibroblast growth factor-2) as active ingredient, asthma being caused by overexpression of IL-13 (interleikin-13) or overexpression of IFN-γ (interferon-γ), where FGF2 inhibits IL-13 activity, FGF2 inhibits VEGF activity and FGF2 inhibits TGF-β1 activity (transforming growth factor-β1). Medication for treatment or prevention of chronic obstructive lung disease (COLD), containing FGF2 (fibroblast growth factor-2) as active ingredient, where chronic obstructive lung disease (COLD) is caused by overexpression of IFN-γ (interferon-γ). Inhibitor of IL-13 activity, containing FGF2 (fibroblast growth factor-2) as active ingredient. Inhibitor of VEGF activity, containing FGF2 (fibroblast growth factor-2) as active ingredient. Inhibitor of TGF-β1 activity, containing FGF2 (fibroblast growth factor-2) as active ingredient.
EFFECT: prevention of asthma and COLD.
8 cl, 31 dwg, 7 ex
SUBSTANCE: method relates to medicine, namely to oncology, and can be used for treating patients with locally spread or generalised lung cancer. Essence of invention lies in the following: sample of bone marrow from posterior spine of iliac crest in amount 150-180 ml is taken, incubated at 37°C during 24 hours with 1 mln ronkoleikin IU (IL-2), after that autohemochemical therapy (AHCT) is performed together with automieloimmunotherapy (AMIT), medications are introduced intravenously with drip-feed, two courses of treatment are performed with 3 week interval. In case of small cell cancer the following medications are introduced: cysplatin - 80 mg/m2 on the 1-st day, vepesid - 120 mg/m2 on the 1-st, 2-nd, 3-rd day, ronkoleikin - 1 mln IU on the 2-nd, 3-rd, 4-th, 5-th day. In case of non-small cell cancer the following medications are introduced: cysplatin - 80 mg/m2 on the 1-st day, vepesid - 120 mg/m2 on the 1-st, 3-rd, 5-th day, ronkoleikin - 1 mln IU on the 2-nd, 4-th, 6-th, 7-th day.
EFFECT: method allows enhancing anti-tumor activity of immune system and stabilising neoplastic process.
3 cl, 2 ex
FIELD: food industry.
SUBSTANCE: invention relates to primary processing of medical-and-technical raw materials, namely to preservation of reindeer unossified horns (antlers). Reindeer unossified antlers preservation method includes freezing, thermal treatment using infrared radiation spectrum till normal moisture content is reached. Unossified antlers, frozen at -20-24°C are sawn into plates 0.5-0.7 mm thick, put on reticulate cassettes (without defrosting) and thermally treated with active ventilation at wave length 1.6-2.4 mcm, 6 hours and at 45°C. After that they are left for cooling during 12 hours, plates are turned over and thermal treatment process is repeated till production with moisture content 12-19% is obtained.
EFFECT: invention provides for production of high-quality raw material with high content of biologically active substances, reduction of technological operations amount and preservation time.