Penicillium verrucosum fungal strain, used for making agent with immunomodulating properties, and immunomodulator agent based on said strain
FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology and design of agents with immunomodulating properties. A new fungal strain Penicillium verrucosum VKPM F-984 and a new immunomodulating agent based on the strain are proposed. The strain is extracted from microflora of ginseng roots and is kept in a medium which contains mineral salts, glucose and asparagine. Fungus mycelium is extracted with a water-alcohol solution (70% ethanol solution). The advantage of this agent is its natural occurrence and effecient stimulation of adaptive capabilities of the body.
EFFECT: obtaining an extract which has stimulating action on cell and humoral immunity, improves immune status of the body.
3 cl, 2 ex
The invention relates to biotechnology, in particular to the development of new sources of biologically active substances and immunomodulating drugs for effects on a living organism.
Currently great interest in drugs of natural origin, their advantage is their physiology to the body and environmentally friendly.
Known biologically active preparations immunostimulating action, obtained from natural sources (based on Symbiotropin fungi)that live in natural conditions on the roots of plants, such as drugs Emistim C, agreementin-Extra [1, 2]. These dyes contain optimal for stimulation of plant life set of growth substances (hormones), vitamins, amino acids, fatty acids, trace elements. Multiple trials of these drugs have shown that they are improving the germination of seeds of plants, resistance to diseases and stress factors, increase yield and improve the quality of crop production, reduce the nitrate content . This is due to the activation of the fundamental life processes of plants - photosynthesis, respiration, nutrition of cells, activation of enzyme systems. These drugs are made from natural materials, obtained the mushroom-epiphytes root system of medicinal plants, certified and certified for environmental safety.
It is also known biologically active agent (Biocorrector) "Floravit e" on the basis of the extract of the fungus Fusarium sambusinum, which is an effective immunomodulator and adaptogen . This tool is widely used in clinical medicine as in the treatment of various diseases (diabetes, trophic disorders, diseases of the joints), and in the fight against viral infections. Clinically shown its hepatoprotective action, efficacy of gastroenterology, obstetrics-gynecology and for healing and rejuvenation in rehabilitation medicine and cosmetics . Therapeutic possibilities of preparation "Floravit e" connected, primarily, with its natural origin of the fungus Fusarium sambusinum and unique set of biologically active components in its composition.
However, finding natural sources to search for and create a highly biologically active agents, with a unique set of constituent components and its influence on the organism of man and animals, is a difficult task. The selection of a biologically active substance from a source of natural origin must also ensure maximum preservation of useful properties, UTS is DTIE toxicity and side effects.
The solution to this problem is possible using the new tools of natural origin, have immunomodulatory properties, that can be used in a variety of therapeutic effects.
This is achieved by using as the source of natural raw materials of a new strain of the fungus Penicillium verrucosum VKPM F-984 and the allocation of a drug with immunomodulatory properties.
A new strain of the fungus Penicillium verrucosum VKPM F-984 used to obtain funds, have immunomodulatory properties, and the means obtained on the basis of the strain Penicillium verrucosa VKPM F-984, have immunomodulatory properties. This tool is obtained, in particular, the extraction of the mycelium of the specified strain and is a water-alcohol extract.
The technical result of the claimed group of inventions is finding new natural sources of biologically active substances and expanding range of high-performance tools-immunomodulators natural origin, designed to impact on a living organism in the field of medicine, veterinary medicine or holistic health events. Use them to more effectively carry out the treatment and prevention of diseases by improving immunity and overall impact on the body.
is received from a strain of the fungus Penicillium verrucosum VKPM F-984 immunomodulatory agent possesses adaptogenic action, increases the nonspecific resistance of the organism and its adaptive capacity. To obtain usually carry out the extraction of the mycelium of the fungus in an aqueous solution of alcohol.
The strain of the fungus Penicillium verrucosum VKPM F-984 isolated from the microflora of ginseng roots as follows.
The method of strain isolation: ginseng roots washed under running water, dried on filter paper and taken roots 4-5 procedure, 5 g of dry roots treated with 60% H2SO4, crushed and transferred to flasks with culture medium (nutrient medium composition: glucose - 8.0 g, K2HPO4- 0.5 g, KH2PO4- 1.0 g, MgSO4- 0.2 g, K2SO40.1 g l - asparagine-20 mg, water water up to 1 litre).
For storage and reproduction of endophytic micromycete Penicillium verrucosum VKPM F-984 use apparitional environment: glucose - 8.0 g, K2HPO4- 0.5 g, KH2PO4- 1.0 g, MgSO4- 0.2 g, K2SO40.1 g l - asparagine-20 mg tap water to 1 liter, agar-agar - 15,0,
The culture is plated on Petri dishes, incubated in thermostat and used for preparation of inoculum.
The selected strain was deposited in the collection of the Depositary PMBC, he assigned a registration number F-984.
Kulturalna-morphological properties of the strain.
Micromorphologically signs: when planting a shot on medium with agar on day 5 of growth at +24°C develops warty colony rounded grey-green, on the periphery of the weak striation, the size of the colony diameter of 35-40 mm, aerial mycelium-reverse white, surface sporulation, the color of the reverse side of the colony brownish-yellowish. Mycelium colorless septate, a large number of small round greenish conidia, conidiophores onomatopoeia monoarticular, spores spherical shape, transparent, smooth shell.
Physiological and biochemical properties of the strain.
The strain grows in a temperature range from 24 to 27°C, the temperature optimum of 25-27°C.
When growth on nutrient media preferred slightly acidic pH values, the pH optimum of 6.5.
Can be grown in simple environments using glucose as a carbon source, preferably the content in environments potassium and magnesium.
The method of obtaining funds from the strain of Penicillium verrucosum VKPM F-984 includes the following operations.
The culture fluid with mycelium with stirring, treated with activated charcoal (5 g/l liquid), incubated for 25 hours and filtered. Solid fraction at intensive stirring and extracted with an aqueous solution of alcohol (70%) during the day. The extract is filtered.
The obtained filtered alcohol extract is described by means of having immunomodulatory properties.
Example 1. Receiving immunomodulator is C strain Penicillium verrucosum VKPM F-984.
In fermentors under sterile conditions, add seeds at a dose of 3% of the volume of the fermentation medium. Fermentation environment includes: glucose - 8.0 g, K2HPO4- 0.5 g, KH2PO4- 1.0 g, MgSO4- 0.2 g, K2SO40.1 g l - asparagine-20 mg tap water to 1 liter fermentation is finished for the maturation of the mycelium of the fungus and on the achievement of the maximum accumulation in the culture fluid of metabolic products of the fungus-micromycete.
The obtained culture liquid with mycelium under intensive stirring, treated with activated charcoal at a rate of 5 g per 1 l of the liquid is maintained at a temperature of 25°C for 24 hours and filtered. The solid fraction is placed in a separate container and with vigorous stirring extracted the estimated amount of 75%aqueous ethanol solution for 24 hours. Upon completion of the extraction are filtering and receive the filtered extract.
Example 2. The study of the properties of the obtained immunomodulator.
To study the effect of the drug on hematological, biochemical and immunological parameters of the body was an experiment with a duration of 6 months in rats male WISTAR rats with an initial weight of 125-130, Rats were divided into 2 groups: the control group animals received obsessively diet, experience the Naya group animals on the background obseving daily diet along with food received drug-immunomodulator dose of 0.25 ml/kg weight of the animal. Conducted 2 slaughter animals: after 3 and 6 months from the beginning of the experiment.
The influence of the drug-immunomodulator on morphological characteristics of the organism.
The General condition of the animals of all groups was satisfactory throughout the experiment.
Conducted microscopically studies 10 bodies from each rat: liver, kidneys, lungs, testes, lymphoid organs (mesenteric lymph node, spleen and thymus gland, thymus) digestive tract (thin and thick intestine, stomach). After 3 months from the beginning of the experiment there was a slight degenerative changes in the liver in the experimental group compared with the control. In the spleen and the mesenteric lymph node in some cases it was noted lymphoid proliferation in the experimental group compared with the control. After 6 months from the beginning of the experiment was not found significant morphological changes of the internal organs in the experimental group compared to control.
The effect of the drug on the biochemical and hematological parameters of blood and urine.
In the study of biochemical parameters take into account the total protein content, glucose content in the blood serum, the activity of some enzymes in the blood serum and liver slid the, the content of creatinine in the urine.
The content of total protein in the serum of rats, the enzyme activity of blood serum and liver of rats when their diet immunomodulatory agent obtained in example 1, did not differ from controls throughout the experiment (after 3 and 6 months).
Not identified differences between the experimental group and the control on such parameters as the concentration of hemoglobin, the total number of erythrocytes, hematocrit, CCO, sste throughout the experiment. Leukocyte formula in rats when their diet study drug did not significantly differ from the control.
Immunotoxic properties of the drug.
Immunotoxic properties of the drug were studied in the experiment on white mice-males weighing 18-20 g
On the influence of the drug on cellular immunity was assessed by the severity of hypersensitivity reactions of the delayed type and autologous rosette cells (autologous ROCK).
Determination of severity of hypersensitivity reactions of the delayed type (GTT) was performed in 40 mice CBA. The first group of animals were administered the study drug at a dose of 0.25 ml/kg, 2-nd group - at a dose of 0.5 ml/kg, 3rd group - 1 ml/kg for 5 days oral, all experimental groups on day 5 in parallel with conducting drug was administered suspension of sheep erythrocytes (EB) nutriplus the NGOs (10% suspension) at a dose of 0.3 ml/kg Animals of the control group was injected only 10% suspension of DL. 5 days after sensitization all experimental animals were injected with a resolving dose of 2% suspension of DL in the amount of 0.02 ml in the right foot, and left in the saline solution and 0.02 ml of the Control animals were injected only 2% suspension of EB and took into account the stimulation index. When the stimulation index was established that the study drug has immunostimulating properties in all tested doses (of 0.25; 0.5; 1.0 ml/kg body weight) and stimulation index (IP) was 9,8±0,7; 10,1±0,2; 9,45±0,4 accordingly, groups of experimental animals in the control IP=4,5.
Changes in the dynamics of auto-ROCK animals on the background of the introduction of the drug was studied on 75 mice CBA. Group 1 mice received the drug at a dose of 0.25 ml/kg, 2-I in a dose of 0.5 ml/kg orally for 5 days, group 3 served as a control. Studies were performed at 5, 10, 15, 20 days after the last injection of the drug. Kinetics of auto-ROCK was characterized by an increase in both absolute and relative number of rosette cells in the blood of mice.
Changes in the dynamics of indicators of autologous rosette cells in mice provides insight into the impact of the drug on cellular immunity, resulting in increased functional activity of T-lymphocytes. Evaluation of In-cell component of the immune system was carried out according to definition number is the number of antibody productive cells in the spleen and the kinetics of b-lymphocytes.
Determination of antibody productive cells (AFC) in the spleen was performed in the experience of 30 mice CBA. The first group of animals, the drug was administered at a dose of 0.25 ml/kg, 2 to 0.5 ml/kg, intraperitoneally simultaneously introduced the EB. The number of KLA believed in one sample among 200 lymphocytes. The drug induces the transformation of b-lymphocytes in the AFC in the spleen, the stimulation index (IP) is equal to 1.4 in the control group IP amounted to 0.9.
The kinetics of b-lymphocytes was determined in the experience of 60 mice CBA. Contents EAC-rosette cells in the blood of the mice of the control group did not significantly differ from the experimental group during the whole research period and amounted to 19,4±2,6-20,9±1,4.
In the dynamics of the relative amount of b-lymphocytes after drug maintenance dose of 0.25 ml/kg was observed the significant increase in the EAC-ROCK on the 10th and 15th days of the study, while in the 2nd group of animals (dose 0.5 ml/kg) on 5-th, 10 th, 15 th days of observations the contents of the EAC-ROCK amounted to 23.6±1,18-22,6±1,13. The data obtained allow to conclude that the investigational drug in a dose of 0.5 ml/kg, administered orally for 5 days increases cellular immune response.
Determination of hemagglutinin titers in the serum of animals after administration of the drug was carried out on 30 mice CBA (2 experimental groups, 1 control group). On day 7 after immunization all belly is s were scored and determined the titers of total antibodies and antibody classes M and O. The content of immunoglobulins M and O in the experimental groups was higher than in the control.
Immunological studies in rats.
Determination of immune status was assessed by the competence of the cellular and humoral immune response in rats male WISTAR rats with an initial weight of 125-130 g in chronic Toxicological experiment with a duration of 6 months. Rats were divided into 2 groups: the control group animals received obsessively diet, experimental group of animals on the background obseving diet along with food received study drug at a dose of 0.25 ml / kg of body weight of the rat.
The competence of cellular immunity was assessed by induced phytohemaglutinin (PHA) and specific antigen prepared from the investigational drug reactions stimulation of peripheral blood lymphocytes, the expression of response inhibition of leukocytes in the presence of PHA and specific antigens and the degree T-dependent cellular cytotoxicity. Mitogenic response of peripheral lymphocytes of rats to PHA and specific antigen (CA) were evaluated for the percentage of transformed cells. A study of the influence of the drug on the mitogenic stimulation of peripheral lymphocytes of rats, it was found that it can increase the proliferative activity of T cells. The value of the Indus the KSA cytotoxicity in rats of the experimental group after 3 months amounted to 0.35±0.02 and after 6 months of 0.32±0.03 in, and in the control group, respectively, and 0.28±0.04 to 0.24±0,01.
These findings indicate immunostimulirutuyu role of the investigational product in relation to thymusdependent lymphocytes.
Competence humoral immunity was assessed by the content in the serum of rats the major classes of immunoglobulins. Research the content of immunoglobulins in the blood serum of rats when their diet drug has allowed to establish the General orientation to the stimulation of humoral immunogenesis. After 3 months, the concentration of immunoglobulin O, a, M, E was in control 743±37,2; 138±6,8; 8±3,9; 0,45±0,01; in the experimental group 861±43,05; 132±6,6; 87±4,5; 0,42±0,03 respectively. 6 months after the beginning of the experiment in the control- 723±36,14; 131±6,5; 80±3,5; 0,45±0,01; in the experimental group- 836±41,8; 136±6,8; 86±4,3; 0,46±0,02 accordingly, by classes of immunoglobulins.
Nonspecific protective indicators studied the content in the serum of some components of the complement and the level of the individual whey proteins acute phase of inflammation. The content of the components of complement NW, C4, C5, C9 in the control was 551+27,5; 239+12,0; 107+5,35: 38±1,9; in the experimental group 567±28,35; 269±13,45; 116±5,8; 46±2,3 respectively.
As a result of the experiments revealed the modulating effect of study drug on cellular immunodeficiency, the major reactions (increased proliferative response of peripheral lymphocytes to mitogens (PHA), the intensity of cytotoxicity). Marked stimulating effect on the synthesis of immunoglobulins (1^0 1^M), components of complement (C4 and C9), proteins of acute phase of inflammation (transferrin, C-reactive protein).
During the research it was revealed that the study drug has not immunotoxic effect in relation to the functional activity of T - and b-cells.
The drug is used in poultry farms to optimize metabolism, enhance immunity and increase productivity. When the feeding of the drug with water, the bird was observed due to the rapid expansion of the mass of broiler chickens, improving the livability of Chicks. The drug activates specific antitelomerase against Newcastle disease in birds, revealed no adverse effects on the clinical status of broiler chickens. Protein quality of meat increased by 3.7%. Thus the use of immunomodulating drug improves zootechnical performance of poultry.
Conducted production tests of the studied drug in animal husbandry has shown its high efficiency as a therapeutic and prophylactic agent in the dyspepsia of newborn calves, diarrhea infectious etiology, bronchopneumonia.
The feeding newborn calves solution is Reparata within 3-10 days at a dose of 0.17-0.25 ml/kg reduced the number of diseased calves 1.6-4.5 times and duration of the disease is 2-4 times. The increase in live weight in the experimental groups outperformed the control animals 3.2-6.2 kg at 30 days of age, at 60 days of age 4.4-8,1 kg the oral application of the drug at a dose of 1 ml per day newborn calf is effective against diarrhea in animals. Livestock safety in experimental groups reached 90-100%.
Thus, the described immunomodulator is not toxic, has a therapeutic effect on a living organism, can be effectively applied in animal husbandry and veterinary medicine for effects on the immune system of animals, increasing the gain and improve the safety of the animals.
Sources of information
1. The biostimulant Emistim STU AT 88.264.021-95.
2. The Biostimulator Of Agreementin-Extra. TU 24.2-31168762-001-2004.
3. Pokrovskaya ST Ways to reduce the nitrate content in vegetables. M: Agropromizdat, 1990, s.
4. Catalog-Handbook of Diagnostic and treatment technologies of regenerative medicine, Ministry of health of the Russian Federation, 2004, vol. 1, s, 229, 264.
5. "Dietary Supplement Floravit e in gastroenterology, guidelines / edited by academician of the Russian Academy of natural Sciences, Dr. SC Mahurangi, publishing house of the Russian medical Academy of postgraduate education, 2002.
1. The strain of the fungus Penicillium verrucosum VKPM F-984 used to obtain funds, have immunomodulatory his is Tami.
2. The agent with immunomodulatory properties, characterized by the fact that it is derived from a strain of the fungus Penicillium verrucosum VKPM F-984.
3. The tool according to claim 2, characterized in that it is a hydroalcoholic extract of the mycelium of the strain of the fungus Penicillium verrucosum VKPM F-984.
FIELD: food industry.
SUBSTANCE: invention relates to biotechnology namely to preparation for stimulation of growing and developing of plants based on strain Halobacterium salinarum All-Russian collection of industrial microorganisms B-9025. To obtain the preparation strain Halobacterium salinarum All-Russian collection of industrial microorganisms B-9025 is grown during 6 days. After that biomass is separated by centrifugal process at 7000 g and incubated in distilled water. After that deposit is thrown away and obtained supernatant is centrifugally processed at 50000 g, supernatant is thrown away and distilled water of the same volume is added to the obtained deposit. Deposit is dissolved. Such suspending process is repeatedly performed till ratio of optical density of solution at wave-length 280 nm to solution optical density at wave-length 570 nm (D280\D570) less than 2,5, after that supernatant is standartised till bacteriorhodopsin concentration makes from 10-9 M to 10-6 M.
EFFECT: preparation allows to reduce general fertiliser consumption while growing plants which leads to production of more ecologically clean products with ample quantity of nutrient substances and green mass.
5 ex, 3 tbl
SUBSTANCE: invention relates to biotechnology, particularly to ecology, and can be applied in nature-conservation activity for control of water quality of fresh-water stagnant basins. Method is realised by registration of three biotic and abiotic indices, calculation of trophic index and determining of basin ecological condition by trophic index value, as biotic indices, quantity of algae of Crucigenia tetrapedia (Kirchn.) W. Et G.S. West, Scenedesmus quadricauda (Turp.) Breb. var. quadricauda, Trachelomonas volvocinopsis Swir. are registered, as abiotic indices, average air temperature in ten days, quantity of sediment per ten days and transparence of water in basin, index of trophicity are calculated by definite formula. If value of trophicity index is less or equal 3.6 basin is considered to be mesotrophic, and if value of trophicity index is higher than 3.6 - eutotrophic.
EFFECT: application of claimed method allows fast obtaining of objective and reliable information about ecological condition of fresh-water stagnant basin basing on limited number of tests, which considerably reduces work, time and finance expenditure during research.
4 tbl, 3 ex
SUBSTANCE: invention concerns biotechnology and represents the method for preparing L-threonine with using Escherichia bacterium modified in such a way that tolC gene in specified bacterium is inactivated.
EFFECT: invention enables to prepare high-yield L-threonine.
3 cl, 2 dwg, 2 tbl, 12 ex
SUBSTANCE: invention relates to medical biotechnology, particularly to obtaining cell lines used for making tumour vaccines. A human melanoma cell line mel Si is obtained, which is stored in Specialised collection of spinal cell cultures of the Russian cell culture collection under number RCCC(P)708D.
EFFECT: invention can be used for making tumour vaccines.
SUBSTANCE: invention relates to combined generation of heat and electrical energy. The installation comprises vertical gas-drainage wells 1 with condensate extractors 2 and a stop valve for regulating flow 3 and controlling qualitative composition of dump site biogas 4, connected by gas pipe 5, equipped with gas compressor 6, with high-pressure gas holder-condenser 7, with stop valve 8, non-return gas valve 9, non-return liquid valve 10 and pressure release valve 11, series-connected NaX type adsorber 12 with by-pass branch 13, NaA type adsorber 14 with by-pass branch 15 and heat exchanger 16 for pre-heating enriched biogas fuel with feed branch 17 to a photosynthesising contour.
EFFECT: utilisation of dump site biogas through ecologically safe combined generation of heat and electrical energy.
FIELD: medicine; microbiology.
SUBSTANCE: way is intended for reception of functionally active LF form, the basic toxic protein defining cellular disturbances, leading to death of an organism at infection with a malignant anthrax bacterium. For realisation of the way a recombinant plasmid pETHIS-LF (7816 items) is designed, containing a full-size gene of the lethal factor (LF) of malignant anthrax under the control of the promotor of bacteriophage T7 and to a determinant of ampicillin tolerance. The plasmide provides effective synthesis of LF protein of malignant anthrax merged with sequence of six Histidinums for clearing with the metal-chelate chromatography. The strain Escherichia coli BL-HISLF is designed using transformation of the specified plasmid DNA in the strain E.coli BL21 (DE3), synthesizing active LF protein. The target product is separated with the way including clearing on a metal-chelate sorbent with the subsequent additional clearing of the LF protein by gel-filtration.
EFFECT: reception of active recombinant protein LF on the simplified technology and with a high output of synthesised protein of the lethal factor.
3 cl, 3 dwg, 3 ex
SUBSTANCE: there is disclosed leprosy microbacteria storage method consisting in low temperature effect on the infected substance. The stored substance is cooled at temperature -18°C that allows using household freezers.
EFFECT: simplified method of storage of M leprae.
FIELD: oil and gas industry.
SUBSTANCE: invention relates to receiving of exo-polysaccharides used in the capacity of thickeners during the operation of oil fields, particularly to method of receiving exo-polysaccharides of alginate type. Method provides cultivation of Azotobacter vinelandii "ИБ-1" on culture medium by Fedorov, containing mixture of microelements and molasses in amount 60.0 g/l in fermenter at conditions of aeration; 0.5 of air volumes per 1 medium volume per minute and mixing 300 revolutions per minute at temperature 25°C during 48 hours. Release of exo-polysaccharides by means of sedimentation by isopropyl alcohol in amount 70% by volume.
EFFECT: increasing of exo-polysaccharides yield.
1 tbl, 7 ex
FIELD: medicine; biotechnologies.
SUBSTANCE: method involves cultivation of strain-producer Penicillium canescens chosen from the group including: multicopy strain Penicillium canescens Abf6 (VKM № F-3935D), in gene abfA arabinoxylane-arabinofuranhydrolase Penicillium canescens containing arabinoxylanase and α-L-arabinofuranosidase activity, strain Penicillium canescens AbfB6 (VKM № F-3937D), in gene abfB2 α-L-arabinofuranosidase Penicillium canescens, multicopy strain Penicillium canescens AgLA33 (VKM № F-3936D), in gene aglA α-galactosidase Penicillium canescens containing both α-galactosidase and galactomannanase activity, strain Penicillium canescens AgLC4 (VKM № F-3934D), in gene aglC α-galactosidase Aspergillus awamori, multicopy strain Penicillium canescens FAE9 (VKM № F-3938D), in gene faeA feruloyl-esterase Penicillium funiculosum, multicopy strain Penicillium canescens XG9 (VKM № F-3939D), in gene xegA xyloglucanase Penicillium canescens.
EFFECT: extended range of enzymatic agents.
21 dwg, 1 tbl, 6 ex
SUBSTANCE: present invention relates to biotechnology and genetic engineering. Proposed here is a set of lux-biosensors, consisting of Escherichia coli cells obtained using recombinant DNA technology, containing plasmids with bacterial luxCDABE genes controlled by stress inducible promoters PgpoE and PgrpE and non-inducible Plac promoter.
EFFECT: invention can be used in evaluating degree of contamination of the environment by hydrophobic detergents.
SUBSTANCE: water suspension of yeast cells is prepared. Endogenous enzymes of yeast cells undergo inactivation at 85-95°C for 15-20 minutes. Further deactivated yeast cells are processed with exogenous enzyme complex represented by cultural fluid of micromycete Aspergillus oryzae F-931 of All-Russian Collection of Industrial Microorganisms or concentrated enzyme preparation obtained out of cultural fluid of micromycete Aspergillus oryzae F-931 at 48-52°C and natural pH for 4 hours with constant stirring. Further exogenous enzymes are inactivated at 85-95°C for 15-20 minutes, and obtained enzymolysate is dried in dispersion dryer.
EFFECT: improved quality of yeast enzymolysate.
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SUBSTANCE: there is offered therapeutic agent of systemic action prepared from mycelium of fungus Fusarium sambucinum Fuckel var. Ossicolum (Berk, et Curt) Bilai "ВСБ" 917 (All-Russian collection of industrial microorganisms F-165). The agent represents a concentrated low-molecular peptide compound and defragmented DNA of diametre 1 to 50 nm. The agent contains as follows: mineral elements - about 24% to about 26%, vitamins - about 0.8% to about 0.86%, lipids - about 4.2% to about 7.6%, whole protein - about 55% to about 57%, polysaccharides - about 9.6% to about 14.6%, water - the rest. Besides, there is disclosed application of the therapeutic agent of systemic action to inhibit defective DNA-sequences and to improve replication accuracy of existing DNA-sequences, as well as application of said agent to prepare a pharmaceutical composition. There is also offered method for inhibiting cell enrichment with genetically junk DNA and improvement of defective DNA-sequences and decrease in replication accuracy of existing DNA-sequences with using said therapeutic agent.
EFFECT: agent is characterised with effective biological properties.
20 cl, 11 dwg, 8 tbl
SUBSTANCE: invention concerns biotechnology, particularly methods of obtaining preparation based on chlamydospores of nematophagous microfungus for vegetation and animal parasitic nematode control. Method involves submerged cultivation of microfungus in nutritive medium including carbon and organic nitrogen sources, salts and water, till maximum accumulation of vegetative mycelial mass, introduction of sporulation inducer, with further chlamydospore formation. Duddingtonia flagrans F-882 is used as microfungus, submerged cultivation is performed in gas vortex bioreactor until dry rate of biomass reaches at least 10.0 g/l. To obtain fluid form of preparation, water is added as sporulation inducer, diluting vegetative mycelial mass by 2-5 times, and/or glycerin is added in end concentration of 1-2 wt %, and additionally the biomass is incubated until chlamydospore accumulation in the culture reaches maximum. To obtain dry form of preparation, sterile foamed vermiculite is added to vegetative mycelial mass as sporulation inducer at the ratio of 1.0-4.0 ml of mycelial mass to 1 g of foamed vermiculite, obtained mix is stirred and additionally incubated to obtain chlamydospores.
EFFECT: enhanced efficiency of preparation.
6 cl, 10 tbl, 11 ex
FIELD: medicine; pharmacology.
SUBSTANCE: strain of Myceliophthora fergusii UV-64 VKM F-3932D possesses ability to produce the complex of highly active carbohydrases including neutral cellulase, xylanase and beta-glucanase. It allows receiving variety of enzymes for hydrolysis of non-starched polysaccharides of the vegetative raw materials showing high activity at acescent and neutral value pH.
EFFECT: expansion of assortment of fermental preparations.
3 tbl, 6 ex
SUBSTANCE: method of liquid koji receiving includes cultivation of koji mould in liquid medium, containing in the capacity of raw material vegetable material. koji mould corresponds white or black koji mould. Raw material is selected from grain crops, surface of which is covered by envelope, grain crops, from surface of which it is removed husk, unwrought beans or tubers, surface of which is covered peel, amaranth and/or kinoa. Method provides simultaneous manufacturing and collection of glucoamylase and acid-resistant α-amylase in cultural product.
EFFECT: with usage of liquid koji it can be received ferment alimentary drink shochu.
12 cl, 11 dwg, 40 tbl, 21 ex
SUBSTANCE: Fomitopsis officinalis Tyv-2006 All Russian Collection of Industrial Microorganisms F-961 basidiomycete strain characterised with high activity to malignant cells can also be used for making antineoplastic drugs.
EFFECT: extended range of agents with anticancer activity.
2 tbl, 2 ex
SUBSTANCE: strain of myxomycete Penicillium sizovae P11 is extracted from decaying whitewood. The strain is stored in the gallery of microorganisms of the Vyatsky State University under the registration number VGUCM-P11. Decomposition of lignin is 7.4 wt %.
EFFECT: strain has high ligninolytic activity.
2 dwg, 3 tbl, 3 ex
FIELD: food products.
SUBSTANCE: method of biological active additive production includes three-staged processing of yeast cells water suspension with exogenous ferments to obtain target product in the form of fermentolisate. During the first and the second stage the initial and secondary zymogene compositions are used. The initial zymogene composition contains for 1 g of dried yeast 0.05-5.0 activity of proteinase and 10.0-50.0 activity units of mix of endo-beta-gluconase and exo-beta-gluconase. The secondary zymogene composition contains 50.0-300.0 activity units of mix of endo-beta-gluconase and exo-beta-gluconase, 5.0-20.0 activity units of mannanase and 0.01-0.5 activity units of chitinase. The processing of yeast cells suspension of the initial zymogene composition is performed at 54-56°C during 1 hour 45 min. - 2 hours 15 min. The processing of the secondary zymogene composition obtained after the first stage is performed at 45-48°C during 3 hour 45 min. - 4 hours 15 min. During the third stage processing of the obtained mass is performed with ferments of the secondary zymogene composition at temperature of 35-38°C during 6-12 hours. The product output equals 92%, the concentration of amine nitrogen is 6.3%.
EFFECT: obtaining of target product with high output and high aminoacid content.
8 cl, 3 ex
FIELD: chemistry, biochemistry.
SUBSTANCE: invention refers to biotechnology and agriculture, specifically to fungi growing. Interstrain hybridisation method is applied to produce somatic strain of macroscopic oyster fungus Pleurotus ostreatus (Fr.) Kumm "ВИК" F - 6 being a producer for carposomes of pileate fly edible fungi of carposome productivity 60-70% of dry weight of substratum and high commercial properties. Strain is deposited in "ВКМ" under number F - 3889 D.
EFFECT: higher productivity and improved carposome habitus.
2 tbl, 1 ex
SUBSTANCE: method of obtaining laccase enzyme medicine involves sustaining cultivation parametres depending on dissolved oxygen concentration in medium. Temperature of 25-27°C and pH within 3.0-7.0 are maintained from the start of cultivation until dissolved oxygen concentration falls to 50%. After further decrease of dissolved oxygen concentration to 5% temperature is increased to 27-29°C and uncompensated pH range is narrowed to 4.0-5.0. At reduction of dissolved oxygen concentration to minimum level and its further increase, temperature of 30-33°C and pH of 4.0-6.0 are maintained.
EFFECT: enhanced activity of culture fluid containing laccase.
1 tbl, 3 ex
SUBSTANCE: in formulas (I) and (Ia) . A is 1-cyclopentene-1,2-diyl; Z1 and Z2 are O; E is phenyl substituted with 1-4 substituting groups selected from halogen, Y is phenyl substituted with 1-2 substituting groups selected from halogen, C1-C6alcoxy, halogen C1-C6alcoxy; the meaning of other radicals are indicated in the formula of the invention. The invention refers also to the pharmaceutical composition including the compound of the invention, to the application of the said compound at preparation of the medicinal agent and to the application for DHODH inhibition.
EFFECT: compound of the present invention can find application as medicinal agent inhibiting dehydroorotate dehydrogenase.
9 cl, 18 ex