Immunostimulating and antioxidant composition
SUBSTANCE: invention refers to pharmacology and can be used in veterinary science and medicine for chemotherapy. There is disclosed immunostimulating and antioxidant lithium composition containing lithium ascorbate and lithium aspartate in percentage ratio 50:50.
EFFECT: expansion of the list of low-toxicity immunomodulators applicable for manufacture of drugs.
1 dwg, 2 tbl
The invention relates to chemical compositions based on salts of lithium, possessing biological activity, in particular antioxidant and immunostimulating properties, and can be used in medical and veterinary practice for treatment.
The level of technology
Known bioactive drug ascorbate lithium used as a means for increasing the activity of neutrophils  and has, in addition, antioxidant activity . The composition and the achieved result the tool closest to the claimed and selected as a prototype.
Known from the patent No. 2226391 the drug has a low immunostimulatory activity.
The technical problem of the invention is the extension of the list of therapeutic agents and the development of effective immunostimulating drug based on lithium salts, namely, obtaining a new composition having immunostimulating properties in combination with antioxidant activity.
The problem is solved by the use of lithium ascorbate together with lithium aspartate in the following ratio, wt.%:
ascorbate lithium - 50
aspartate lithium - 50.
Used for solving the problem known component aspartate (asparagine) lithium is used in psycho-neurological clinics is in the treatment of Alzheimer's disease . Its immunostimulatory properties we found for the first time, as available in the patent and medical literature such information does not occur. The composition of the mixture 50:50 chosen from considerations of optimal manifestation of biological activity of the components. We tested other options, however, the variation of the mass fraction of ingredient reduces the activity of one of the target parameters and reduces the overall synergistic effect.
Comparison with the prototype shows that the inventive composition contains distinctive characteristics which together are necessary and sufficient to solve the task.
The invention is implemented in practice and tested in laboratory conditions. When described in the section "summary of the invention the ratio of the components is provided a positive effect on the achievement of the objectives of the technical research task.
The composition according to the invention is used to test the reaction of blast transformation of human lymphocytes (rbtl). With this purpose, conducted the analysis steps of the claimed composition on immunocompetent cells, stimulated phytohemagglutinin (PHA). Investigated the influence of the drug at various concentrations in the range from 0.01 to 0.2 mg/ml Evaluation of the proliferative activity of lymphocytes is spontaneous and induced PHA test performed in accordance with the methodological recommendations of farmkomiteta of the RF Ministry of health (Vedomosti of the pharmacological Committee, 1999, No. 1). Similar tests conducted with drug-prototype (ascorbate lithium).
The results of the testing of drugs in the reaction rbtl shown in the drawing. The testing found that the inventive composition has a stimulating effect on PHA induced lymphocyte proliferative activity. The drawing shows, along with the test without preparation (first two columns, white and black), four charts, two column for the SP-test, two column for PHA-test, respectively, for control of the prototype and the claimed composition. In the control columns have zero height.
From the graphs it is clear that in the area studied concentrations of the inventive composition has a marked stimulatory effect on lymphocytes and the most convincing test of spontaneous proliferation (without activating the influence of PHA), enhancing the effect of 2.2-2.5 times in comparison with the prototype. An important feature of the claimed compositions is the lack of inhibitory effect on the proliferative activity of cells, while the prototype is marked 10-15%suppression activity of lymphocytes compared with controls (drawing).
Investigated the effect of the claimed composition on the functional activity of polymorphonuclear leukocytes in the peripheral blood of donors in a wide range of concentration is rd. Used are known from the literature, the modeling technique of phagocytosis using Staphylococcus aureus N-209 (D.K. Novikov, V.N. Novikov. Assessment of immune status. Moscow-Vitebsk, 1996, 281 C.). Counts the percentage of active neutrophils (PAN) and the percentage of completed phagocytosis (NRF) in the control test without the influence of drugs. The results are presented in table 1, which shows that after exposure of the claimed compositions in the field of the investigated concentration increases the percentage of active neutrophils and phagocytic activity in comparison with the prototype, which opens up the prospect for rational therapy of immunodeficiency.
The antioxidant activity of the composition according to the invention was determined by the cathodic voltammetry, in particular through the process of oxygen reduction (EV 02) . The antioxidant activity of interest was assessed on a temporary or a so-called kinetic criterion of antioxidant activity, which shows the effectiveness of drug interactions with oxygen radicals and is determined by the formula:
where is the criterion of antioxidant activity, µmol/l min;
the oxygen concentration in the original solution without drug, µmol/l;
Ii- mn is an increase in the current EV O 2in the presence of the drug, MCA;
Ibeg- the current value EV O2without the drug in solution, MCA;
T - time of the process, minutes
Research in this procedure the compositions claimed in the percentage of 50:50 showed good antioxidant properties. Results in comparison with ascorbate lithium presented in table 2, which shows that the claimed composition is not inferior to the activity of the prototype with the same concentration values.
In the literature there is no information about the use of the claimed composition in veterinary or medical practice, indicating the novelty of technical solutions, and non-obviousness peak (resonance) ascending immunostimulatory and antioxidant activity of the composition allows to speak about the technical solutions according to the criterion of "inventive step".
|The percentage of active neutrophils (PAN) and the percentage of completed phagocytosis (NRF) when testing the claimed composition|
|Dose, mg/ml||PAN,%||NRF, %|
|The placeholder||Track||The placeholder||Track|
|control without medication||27,1||27,1||32,2||32,2|
|The antioxidant activity of the claimed composition|
|Prototype (ascorbate lithium)||0,02||0,89|
|Declare to notizia||0,02||1,17|
|Cslave- working concentration, g/l;|
|K - kinetic criterion of antioxidant activity, µmol/l min|
Sources of information
1. Carpenters V.M.; Chuchalin B.C.; Skorik N.A.; Ulyanov, S.; Balashov P.P.; Ruzaev LN. Means for increasing the activity of neutrophils. RF patent №2226391, MPK7 AC 31/375. Publ. 2004.04.10 (prototype).
2. Abramchik O.A., Korotkova H., carpenters E.V., Kurbanov Y.A. Study of antioxidant activity and toxicity of ascorbate lithium // proc. Dokl. VII all-Russian scientific conference "Analytics of Siberia and the Far East." Novosibirsk. 2004. S.
3. Pharmaceutical compositions. US Patent No. 4810497, 1989.03.07.
4. Dracheva L.V. and other Study of antioxidant activity of biocomposite based probiotics. The food industry, No. 3, 2007, p.14-15.
The composition having immunostimulatory and antioxidant activity, including ascorbate lithium aspartate lithium in the following ratio, wt.%:
SUBSTANCE: method includes extraction of disintegrated bast of birch-tree bark with water at temperature 95-100°C during 1.5-2.0 hrs., hydromodulus 10-15, separation of the water solution from the bark, its concentration up to 1/4 of initial volume, saturation with sodium chloride and separation of the formed precipitate by filtering. The filtrate is thrice extracted with ethyl acetate, then ethyl acetate extract is concentrated under vacuum up to 1/12-1/18 of initial volume. Concentrated ethyl acetate solution is diluted 5-7 times with chloroform and the proanthocyanidins are separated.
EFFECT: implementation of the claimed objective.
SUBSTANCE: invention covers an agent of microbiological (biotechnological) origin as a cytoprotective, antitoxic, antihypoxic, antioxidant, hepatoprotective, cerebroprotective, cardioprotective agent normalising lipid and energy metabolism. The disclosed agent contains a complex of organic or carbon dioxide extract of yeast used in brewing, bakery and confectionery industry (Saccharomyceus cervaesae).
EFFECT: higher effectiveness ensured by wider range of pharmacological activity of natural phospholipid preparations.
5 cl, 8 ex, 10 tbl
SUBSTANCE: claimed invention relates to derivatives of α-C-phenyl-N-Tret-butylnitrone of general formula (I) , where X represents lactobionamide group, quarternary ammonium group or group ; m represents 1, 2 or 3; y represents 0 or 1; Y represents group -CH2-; m' represents 1; X' represents alkyl chain C4-C14, if necessary substituted with one or several fluorine atoms; Y' represents group selected from functional amide group (-NHC(O)-), urethane group (-OC(O)NH-) or thioester bridge (-S-).
EFFECT: invention also relates to application of claimed compounds for obtaining medication for treatment of pathologies induced by oxidative stress and formation of compounds with free oxygen radicals and to application of formula (I) compounds for obtaining cosmetic composition for prevention and treatment of ageing effects.
9 cl, 4 tbl, 11 dwg
SUBSTANCE: invention concerns medicine and pharmacy area, in particular, to the medical products used for treatment of epilepsy and neuropathic pain. The agent contains carbamazepine and thyotriazoline in a mass parity 1: (0.25-2.5) in quality of an active base according to the invention. The agent possesses anticonvulsant, neuroprotective, nootropic, analgetic, antidepressive, actoprotective and antioxidatic action. Use of the combined agent under the invention allows to raise efficiency of treatment of the specified diseases in comparison with use of carbamazepine and thyotriazoline separately and to lower implication of toxic action of an agent at treatment.
EFFECT: rising of efficiency of treatment of the specified diseases in comparison with use of carbamazepine and thyotriazoline separately and depression of implication of toxic action of an agent at treatment.
9 tbl, 8 ex
SUBSTANCE: invention refers to pharmaceutical and food industry, particularly a corrective composition for pathologic lipid, carbohydrate disbolism and antioxidant organism state disorder. The corrective composition for pathologic lipid, carbohydrate disbolism and antioxidant organism state involvement representing chitosan and ecinochrome A complex, vitamins and organic acids produced from addition to dehydrated and alcohol-processed chitosan of echinochrome A, ascorbic (vitamin C), lipoic (vitamin N), citric and succinic acids in spirit.
EFFECT: composition described above is effective for correction of pathologic lipid, carbohydrate disbolism and antioxidant organism state disorder.
3 cl, 6 tbl, 5 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention concerns a chemicopharmaceutical industry and a medical product for injection containing 6-dekaprenil-2,3-dimetoxy-5-methyl-1,4-benzoquinone (ubiquinone or ubinone) in an organism at preventive maintenance and treatment of various diseases, and also for restoration of performance efficiency and to the method of its obtaining. The method of obtaining consists that certain quantities of a non-ionic surface-active substance and an antioxidant are admixed, heated up to 40-120°C, necessary quantities 6-dekaprenil-2,3-dimetoxy-5-methyl-1,4-benzoquinone are dissolved in the obtained solution, and the obtained admixture at intensive hashing is flown in an isotonic solution preliminary heated to 30-100°C with the subsequent sterilisation of the received composition. The offered invention allows to receive the water-oil emulsions containing 6-dekaprenil-2,3-dimetoxy-5-methyl-1,4-benzoquinone, for injection in an organism.
EFFECT: possibility to reach the maximum concentration of operating substance in blood practically at once.
3 cl, 4 ex
SUBSTANCE: wastes of confectionery industry production, milled husk of cacao beans, are drawn in water at temperature 20°C for 2 hours with following extraction by hot water (80-90°C) in battery of four extractors, thickening of extract and drying are carried out.
EFFECT: extension of medication activity spectrum and decrease of power consumption.
SUBSTANCE: invention relates to (Z)-methyl-16-(5-oxo-2-phenyl-oxazol-4-ilidenmethyl)-15,16-epoxy-8(17),13(16),14-labdatrien-18-oate of formula (I) (I). Compound (I) possesses high antioxidative, hepatoprotective and hemostimulatng activity and can be used for correction of side effects, arising with introduction of highly toxic medications used in anti-tumor therapy.
EFFECT: obtaining compound, which possesses high antioxidative, hepatoprotective and hemostimulatng activity.
1 cl, 4 tbl, 4 ex
SUBSTANCE: invention relates to acceptor of peroxide anion or nitrogen oxide, consisting of highly dispersive platinum powder, which has particle size 6 nm and less observed through microscope. Acceptor is preferably presented in form of water solution containing platinum colloid with ratio 1mM or less per 1000 ml, and can efficiently eliminate excessive level of peroxide anions or nitrogen oxide in vivo.
EFFECT: using particles of such small size allows increasing considerably efficiency of eliminating peroxide anion by means of acceptor.
2 ex, 2 tbl, 1 dwg
SUBSTANCE: dihydroquercitin in amount of 35 mg/kg is injected per os to animals with modeled peritoneal commissures for 7 days. Additionally mexydol is injected intraperitoneally in amount of 70 mg/kg. On 14th day animals are slaughtered for microscopic analysis of peritoneal commissures implications.
EFFECT: prevention of adhesion in abdominal cavity.
SUBSTANCE: invention concerns medicine, particularly therapy, and can be applied in chronic disease treatment. Method involves causative agent extraction or determination of antibodies to causative agents or extraction of genetic components from blood, body fluid or smear. Treatment is performed by vaccine drugs specific to one or several identified causative agents, additionally immunomodulators are administered.
EFFECT: activated repair processes, arrested development of disease state of organs and systems due to elimination of identified pathogen from organism and to immune correction.
3 cl, 8 ex
SUBSTANCE: invention concerns medicine, particularly granulocytopoiesis stimulants, radioprotectors and immune stimulants. Under cytostatics effect Cyclophilin A amplifies granulocyte precursor removal from marrowbone and stimulates granulocytopoiesis.
EFFECT: in case of sublethal radiation, cyclophilin A stimulates migration of marrowbone stem elements and participates in recovery of blood cells and immune system, and shows redioprotection properties.
4 dwg, 2 tbl
SUBSTANCE: medicinal agent contains alpha and/or beta and/or gamma human recombinant interferon, tocopherol acetate or other tocopherol derivatives, ascorbic acid and/or its salts, pantothenic acid or calcium pantothenate, or dexapanthenol, riboflavin or levocarnitine, and orotic acid and/or ornithine or its derivative - citrulline malate, or Glutoxim, antibacterial, antimycotic agents, additives: emulsifiers, stabilisers, preservatives, antioxidants and base. The formulation is suppositories in certain component ratio.
EFFECT: efficiency for severe infectious diseases and mixed infections.
9 cl, 18 ex
SUBSTANCE: invention concerns medicine, be more specific to oncology, and concerns the substances stimulating maturing of dendritic cells (DC). Application of fucoidan from Fucus evanescens or polysaccharide composition from Fucus evanescens consisting of fucoidan in amount of 60-80% and poly-mannuronic acid in amount of 20-40%, as an agent possessing ability to induce maturing of dendritic cells is offered. The declared fucoidan preparations have a standardised composition and, hence, possess direct biological effect. They keep the properties for long time (3 years).
EFFECT: increase of DC functional activity under the influence of the declared substances.
FIELD: medicine, veterinary science.
SUBSTANCE: invention concerns veterinary science, in particular, to ways of reception of immunomodulatory and antiseptic compounds for prevention and treatment of infectious diseases of animals. The way of reception of a complex immunotropic antiseptic preparation for prevention and treatment of infectious diseases of animals includes mixing of an amber acid, novocaine, Dorogov's antiseptic-stimulator of the second fraction and formalin in water at a following parity of components, wt %: amber acid 1; DAS №2 4; novocaine 0.25; formalin 0.4; the distilled water - the rest, and sterilisation using autoclave treatment which is performed in a 1 atm regimen. Within 20 minutes. The received compound possesses antiseptic, immunomodulatory and trophicostimulating activity at a parenteral way of introduction.
EFFECT: rising of efficiency of the received preparation at treatment and prevention of infectious diseases of animals.
SUBSTANCE: invention concerns medicine, namely to creation of immunogene compositions and vaccines for prevention or treatment of the infections caused by Gram-negative bacteria. The immunogene compositions containing a transferrin-binding fiber and Hsf, and a way of their reception are offered. It is shown, that the combination of these two antigens synergically influences on production of antibodies with high activity in the analysis of bactericidal Serum. The composition can be used in vaccines against Gram-negative bacteria, including Neisseria meningitides, Neisseria gonorrhoeae.
EFFECT: creation of immunogene compositions and vaccines for prevention or treatment of the infections caused by Gram-negative bacteria.
56 cl, 10 ex, 1 dwg
FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention concerns area of medical products, in particular to application of complex bonds of iron (III) with carbohydrates or their derivatives for reception of a medical product for improvement of immune protection and-or activity of a brain at patients without an anaemia caused by deficiency of iron or deficiency of iron in which complex bond of iron (III) is iron (III)-polymaltose complex bond or complex bond of iron (III) with a product of oxidation one or several maltodextrins.
EFFECT: development of preparations for improvement of immune protection and-or activity of a brain at patients without an anaemia caused by deficiency of iron or deficiency of iron.
7 cl, 1 tbl, 1 ex
FIELD: medicine; pharmacology.
SUBSTANCE: substance of technical decision seeks to produce immunopotentiating composition based on nanotechnology products, specifically fullerenes and superdispersed diamond powders (nanodiamonds) produced by detonation synthesis. Composition contains modified fullerene of general formula C60-X, where C60 is fullerene nucleus, X is formylporphyrin, as well as superdispersed diamond powder in ratio as follows, wt %: porphyrin modified fullerene C60 - 50; superdispersed diamond powder - 50.
EFFECT: synergetic effect of immunopotentiating components activity with simultaneous considerable reduction in price concerning prototype composition.
2 tbl, 1 dwg
SUBSTANCE: autoblood is daily sampled in patient as 2 ml/kg of weight. Sampled blood is mixed with 20 ml of 0.06% sodium hypochlorite with further ultraviolet radiation with the help of "Isolda" device for 20 minutes. Then blood is intravenously injected to by drop infusion. Treatment is carried out daily for 5 days.
EFFECT: enhanced efficiency of treatment of patients with chronic obstructive bronchitis.
2 ex, 2 tbl
FIELD: medicine; immunology.
SUBSTANCE: human immune system is stimulated with using osmium iridium mineral dispersed to 0.1 mcm. Then dispersed osmium iridium is separately mixed with medicinal bitumen in ratio 1:1000. Produced composition is processed in vacuum chamber at pressure 0.01-0.001 atm and temperature 20-30°C within 2-4 hours and in autoclave at pressure 5-10 atm and temperature 60-80°C within 5-10 hours. Produced composition is injected to human body.
EFFECT: method allows for immune system stimulation, improved organism protected from adverse factors affecting self-regulation and self-regeneration processes.
SUBSTANCE: invention relates to veterinary. Medication for treatment and prevention of diseases, induced with deficit of selenium for agriculture animals, includes sodium selenite, levamisole base and ascorbic acid and water for injections, with following component ratio in wt %: sodium selenite 0.05-0.4; levamisole base 3.0-8.0; ascorbic acid 5.0-15.0; water for injections - the remaining part.
EFFECT: invention provides immunoprotecting, anti-stress and antioxidant action of medication and reduction of costs for its production.
8 tbl, 4 ex, 1 dwg