Early diagnostic technique for tracheobronchitis in calves

FIELD: veterinary science.

SUBSTANCE: invention refers to veterinary science. The technique implies intravenous introduction of 0.6% hydrogen peroxide in 0.9% saline solution in a dose 0.4 ml per kg of body weight. In case cough reaction is observed 1-7 minutes after introduction, tracheobronchitis is diagnosed.

EFFECT: invention allows accelerating diagnostics of tracheobronchitis in calves.

8 tbl, 3 ex

 

The invention relates to the field of veterinary medicine and relates to a method of early diagnosis of tracheobronchitis in calves.

Tracheitis - inflammation of the mucous membrane of the trachea. The animals in most cases are not registered as an independent disease, and as a complication of laryngitis (laryngotracheitis) or bronchitis (kennel cough).

Bronchitis - inflammation of the mucous and submucous membrane of the bronchi. Along the course there are acute and chronic bronchitis. Depending on the localization of the inflammatory process in the bronchi of different size are macropolicy, when the inflammatory process is localized in the large bronchi, microbranches, when the process involved small bronchi, and diffuse bronchitis, when the inflammation spreads throughout the bronchial tree. Depending on the nature of the inflammatory exudate bronchitis may be catarrhal, fibrinous, purulent, septic and hemorrhagic [1, 2].

Among the respiratory diseases of calves viral etiology of great importance parainfluenza-3 (PG-3) and infectious bovine rhinotracheitis (RTI), which occur in the form of mono-and mixed infektsii, e.g. keratoconjunctivitis, allergic rhinitis, tracheitis, bronchitis, catarrhal bronchopneumonia, often turning into interstitial complications of conditionally pathogenic microflora [3, 4].

The known is a method for the diagnosis of tracheitis: the diagnosis is based on examination, palpation, auscultation and x-ray studies.

Infectious bovine rhinotracheitis, parainfluenza-3 conduct serological, virological and (or) molecular genetic studies, and in the case of complications of bronchopneumonia and bacteriological [2, 5].

However, this method involves clinical and laboratory research, and does not allow to identify subclinical course tracheobronchitis.

There is a method of identifying tracheobronchitis, including palpation of the first tracheal ring, which in the case of high sensitivity of the mucous membrane is logged cough [6].

The disadvantage of this method is that the sensitivity of the first tracheal ring and all of the trachea is logged in patients with severe tracheitis and bronchitis (dry or wet cough, hard vesicular breathing, wheezing, expiratory dyspnea, and so on).

It is known that the sensitivity of the mucous membrane of the larynx, trachea and bronchi inflammation increased. Palpation in this case causes a cough and General motor response of the animals. In healthy cattle cough reaction to cause almost impossible [6].

There is also known a method for the diagnosis of pulmonary hypertension. The method is based on the intravenous injection of 0.3% solution of hydrogen peroxide and applied medical is ractice [7].

The closest to the nature of the claimed method is a method that includes palpation of the last tracheal ring, in case of hypersensitivity is diagnosed early manifestation of tracheobronchitis [8].

The disadvantage of this method is that palpation of the last tracheal ring does not allow to identify increase the sensitivity of the mucous membrane of the bronchi, which ultimately does not allow diagnosis of tracheobronchitis in the earlier stages of the disease.

The technical result of the invention is the acceleration of diagnosis tracheobronchitis in calves.

The method is as follows. Calves injected with 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution at a dose of 0.4 ml per kg of body weight. In case of cough response through 1-7 minutes after administration is diagnosed with kennel cough.

Data analysis in the scientific and patent literature revealed no method for early diagnosis of tracheobronchitis in calves using intravenous injection of 0.6% hydrogen peroxide solution, which indicates that according to the claimed solution of the criterion of novelty.

The invention can be used for early diagnosis of tracheobronchitis in calves in large livestock complexes and small-scale farms, which is very important for livestock is veterinary specialists for timely treatment and preventive measures to treat tracheobronchitis, monitor the effectiveness of treatment of calves with respiratory pathology.

The method allows to identify tracheobronchitis in calves 2-6 days earlier than when using prototype [8]. In addition, intravenous administration of 0.6% hydrogen peroxide solution in 0.9% solution of sodium chloride contributes to the activation day-to-day metabolism.

Early diagnosis tracheobronchitis using the proposed method were carried out by LLC "Warniproduct" novousmansky district of Voronezh region 64 calves 1-1,5 months of age.

The essence of the method is illustrated by examples

Example 1. The rationale for the use of hydrogen peroxide to identify tracheobronchitis in calves.

In agriculture, the long-term unfavourable PG-3 and RTIs, often occurring in the form of tracheobronchitis, took 40 calves 1-1,5 months of age, 14 of them with respiratory pathology (tracheobronchitis, pneumonia) - the first group and 26 clinically healthy calves - the second group.

Calves intravenously injected with 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution at a dose of 0.4 ml per kg of body weight. In case of cough response through 1-7 minutes after administration was diagnosed with kennel cough.

After the animals for 7 days led constant clinical observation, defined body temperature, the number of respiratory movements, SOS is the right of the mucous membranes, the sensitivity of the larynx and trachea, cough, wheeze, the presence or absence of dyspnea, nasal effusions, recorded the time of appearance of the sensitivity of the last tracheal ring, reaction calves in the administration of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution. The results of the experiment are presented in table 1.

Table 1
The reaction of the calves on the introduction of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution.
IndicatorsCalves
Group 1 (patients)Group 2 (healthy)
The number of calves heads (%)14 (100)26 (100)
One of them reacted to the introduction of a 0.6% solution of hydrogen peroxide, heads (%)14 (100)12 (46,2)
One of them reacted to palpation of the first tracheal ring, heads (%)14 (100)-
palpation of the last tracheal ring, heads (%)14 (100 -
The time of appearance of the sensitivity of the last tracheal ring, the daypalpation3,8±0,46

From table 1 it is evident that clinically healthy calves on palpation of both the first and the last tracheal rings did not respond. However, the introduction of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution at a dose of 0.4 ml per kg of body weight of 26 calves in 12 (46.2 per cent) were registered cough (from 2 to 7 cough tremors). While the sensitivity of the last tracheal ring these calves after administration of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution showed 3.8±0.46 days.

In calves with clinical form of tracheobronchitis as palpation of the first and the last tracheal rings, and intravenous 0.6% hydrogen peroxide solution, 0.9%sodium chloride solution was observed coughing (from 3 to 9 cough tremors) with sputum.

That is, intravenous administration of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution calves 3.8±0,46 days earlier reveals subclinical course of tracheobronchitis in comparison with palpation of the last tracheal ring.

Clinical and biochemical parameters are presented in table 2.

Table 2
Clinical and biochemical parameters in healthy and diseased calves.
IndicatorsCalves
SickHealthy
Reacting 0.6% hydrogen peroxideNot reacting 0.6% hydrogen peroxide
Body temperature, °C39,8±0,22*39,0±0,0638,7±0,06
The frequency of respiratory movements per minute54±4,7*35±3,229±3,1
Heart rate, in minutes118±10,3*91±9,386±5,2
Leukocytes, 109/l8,3±0,9010,1±1,018,9±0,68
Erythrocytes, 1012/l5,4±0,116,0±0,145,9±0,11
Hemog the Aubin, g/l81,2±1,97*to 83.7±3,7088,2±3,72
Protein, g/l61,72±2,6163,14±2,3762,31±1,83
Lipids, g/l3,25±0,343,43±0,173,72±0,29
Cholesterol, mmol/l3,17±0,34*4,00±0,46or 4.31±0,42
Creatinine, mmol/l78,3±4,59from 71.3±4,2972,6±3,05
Glucose, mm/l2,20±0,43*3,31±0,38*4,72±0,44
Urea, mmol/l3,90±0,28*3,57±0,71*2,19±0,23
Catalase, μm N2About2/(l×min)31,83±1,40*33,66±1,1935,54±1,69
SOD, cu0,52±0,06*0,75±0,100,78±0,14
Gap mm GSH/(l×min) 25,34±0,7526,36±1,1324,92±2,10
HMM, µm/l1,17±0,07*1,18±0,09*0,87±0,05
MSM $ 20,47±0,97*16,17±0,7514,69±0,54
SSE, %53,83±1,8950,42±1,9450,61±1,69
Note: * - P<0,05-0,001 compared with calves not responding to intravenous administration of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution.

Table 2 shows that in clinically healthy calves, reacting to the introduction of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution (positive cough reaction), in comparison with calves, not reacting to the introduction of 0.6% hydrogen peroxide solution in 0.9% solution of sodium chloride, the body temperature is higher by 0.3°C, respiratory rate of 6 breaths / minute, heart rate of 5 beats per minute, the number of cells above 1.2·109/l (13.5 per cent), the hemoglobin concentration of 4.5 g/l (5,1%), cholesterol by 0.31 mmol/l (7.2 per cent), although these differences are not statistically significant. the same time the glucose content in the blood was significantly lower in 1.43 times (P< 0.05), and urea above 1.63 times (P<0,05), respectively.

Between the activity of antioxidant enzymes (catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPO)) blood in clinically healthy calves, reacting 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution and areagirls calves, no statistically significant differences. While the concentration of malondialdehyde (MDA), a secondary product of lipid peroxidation (LPO), in healthy calves, reacting to the introduction of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution, 1.36 times (P<0,05) higher compared with calves that administration of 0.6% hydrogen peroxide solution in 0.9% solution of sodium chloride did not react. Indirectly, this may indicate increased formation of reactive oxygen species (the superoxide anion,- hydroxyl radical and H2O2- hydrogen peroxide) in calves, responsive to intravenous administration of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution. In physiological concentrationsdoes not change the diameter of the trachea and bronchi, and H2O2andcause contraction and relaxation, respectively. In pathological concentrationscauses relaxation is the situation of the trachea and bronchi, and N2About2andcause majority of Contracting, and that manifests itself in the form of coughing reaction [9, 10]. In clinically healthy calves, not reacting to the introduction of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution, its introduction does not exceed physiological concentrations of N2About2and, therefore, does not cause Contracting trachea and cough reaction. In clinically healthy calves, reacting to the introduction of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution, the introduction of additional hydrogen peroxide in the bloodstream leads to the creation of pathological concentrations of N2About2and, therefore, causes the predominant Contracting trachea and bronchi, against the background of subclinical increase the sensitivity of the mucous membrane of the trachea and bronchi provokes cough response.

The indicators characterizing the state of the system FLOOR-AOD 48 hours after intravenous injection of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution, presented in table 3.

Table 3.
The activity of the main antioxidant enzymes and concentration of secondary peroxidation products in the blood in calves 48 hours after nutrive the aqueous injection of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution.
IndicatorsClinically healthy calves
Reacting 0.6% hydrogen peroxideNot reacting 0.6% hydrogen peroxide
Catalase, μm N2About2/(l×min)42,79±2,19*37,55±2,74
SOD, cu0,81±0,210,63±0,16
Gap, mm GSH/(l×min)26,07±0,73of 28.96±0,37
HMM, µm/l0,98±0,08*0,88±0,05
Note: * - P<0,05-0,001 compared with the original values.

From table 3 it can be seen that 48 hours after intravenous injection of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution in calves, reacting to the introduction of the cough reaction, catalase activity significantly increased by 27.1% (P<0.05), and the concentration of MDA in the blood is reduced by 20.4% (P<0.05) as compared with the original values, which indicates an increase in the activity of enzymatic antioxidant link system and the reduction of blood secondary products the OL.

Indicators sorption ability of erythrocytes (SSE) and the content weight products of proteolysis in the blood of the so-called molecules of average weight (MSM), reflecting the influence of oxidative stress on biological membranes and the degree of endogenous intoxication, presented in table 4.

Table 4.
Indicators sorption ability of erythrocytes (SSE) and the content weight products of proteolysis in the blood of calves within 48 hours after administration of 0.6% hydrogen peroxide solution in 0.9% solution of sodium chloride
IndicatorsClinically healthy calves
Reacting 0.6% hydrogen peroxideNot reacting 0.6% hydrogen peroxide
MSM $ 14,88±0,9114,86±0,70
SSE, %48,16±1,5647,58±2,45

Table 4 shows that all clinically healthy calves 48 hours after intravenous injection of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution has tendencies the decrease in the sorption capacity of red blood cells, and calves, reacting to the introduction of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution, and the concentration in the blood of MSM, indicating a decrease in the degree of endogenous intoxication and the improvement of biomembranes.

The content of urea and glucose in the blood at 48 hours after administration of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution are presented in table 5.

Table 5.
Glucose and urea in blood 48 hours after intravenous injection of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution.
IndicatorsClinically healthy calves
Reacting 0.6% hydrogen peroxideNot reacting 0.6% hydrogen peroxide
Glucose, mm/l5,13±1,20*lower than the 5.37±0,54
Urea, mmol/l2,35±0,54*1,63±0,27
Note: * - P<0,05-0,001 compared with the original values

Table 5 shows that 48 hours after the introduction the of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution in calves, reacting to the introduction of the cough reaction, the concentration of glucose in the blood is increased by 55,0% (P<0.05), and the concentration of urea in serum is reduced by 65,8% (P<0,05). In calves, not reacting to the introduction of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution, tends to increase the level of glucose and reduction of urea in the blood. The data indicate improvement of metabolism and oxidant-antioxidant status in calves after 48 hours after the intravenous injection of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution at a dose of 0.4 ml per kg of body weight.

Example 2. The rationale for the relationship of early diagnosis of tracheobronchitis in calves with titers of antiviral antibodies.

On the farm permanently affected GHG-3 and RTIs, selected 10 calves 1-1,5 months of age who do not respond to palpation of the last tracheal ring, but responds to intravenous 0.6% hydrogen peroxide solution in 0.9% solution of sodium chloride cough reaction (subclinical tracheobronchitis). For the animals during the month led clinical observation, defined body temperature, heart rate and respiratory movements were taken into account, the appetite, the condition of the mucous membranes, the sensitivity of the larynx, trachea and the intercostal spaces, the nature of cough, wheezing, breathlessness, nasal istace is s, the time of occurrence and duration of disease tracheobronchitis, take into account complications such as pneumonia. In calves at the age of 1-1,5 1,5-2 months took blood for determination of antiviral antibodies. Titers of antiviral antibodies in calves is presented in table 6.

Table 6.
Titers of antiviral antibodies in calves when tracheobronchitis.
№ p/pThe titers of antibodies to the antigen of the pathogen
PG-3RTI
SubclinicalClinically pronouncedSubclinicalClinically pronounced
11:201:201:21:32
21:401:801:41:4
31:801:1601:8 1:16
41:801:160Ref.1:8
51:1601:320Ref.Ref.
61:1601:1601:161:16
71:801:3201:81:16
81:1601:320Ref.1:8
91:401:801:41:8
101:1601:4801:41:16
cf.1:981:2101:4,61:10,8

From table 6 it is seen that the average titer of the pathogen PG-3 calves, nariakira on palpation of the last tracheal ring, but unresponsive to intravenous administration of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution at a dose of 0.4 ml per kg of body weight of the animal (subclinical course tracheobronchitis)is 1:98, when the clinical course is 1:210, and exciter RTI is 1:4.6 and 1:10,8 respectively.

However, detailed analysis revealed that in calves№2, 3, 4, 5, 7, 8, 9, 10 there is an increase in the titer of antibodies to the antigen of the pathogen PG-3 (average from 1:100 to 1:240). Calves 1 and 6 it remained unchanged. In addition, calves№3, 4, 7, 8, 9, 10 the marked increase in the titer of antihemagglutinin to the antigen of the pathogen RTIs and calves No. 2 and 5 it remained unchanged.

That is, all calves with clinical manifestation of tracheobronchitis is registered or increase in antibody titers to one or more pathogens of acute respiratory diseases (PG-3 and RTIs) or their value remains unchanged.

The time of occurrence and duration of respiratory diseases in calves are shown above in table 7.

Table 7 shows that, on average, in calves, not reacting to palpation, but responds to intravenous administration of 0.6% hydrogen peroxide solution in 0.9% sodium chloride at a dose of 0.4 ml per kg of body weight of the cough response, indicating subclinical tracheobronchitis, the latter was detected by 36.4±1,68 day, and to onicescu its manifestation (the mucous membrane of the nose, conjunctiva, naso-GURNEE mirror hypermonogenic, with erosions, serous discharge from the nose, increased sensitivity of both the first and the last tracheal rings and larynx, auscultation tapped dry and moist rales, marked expiratory dyspnea) 44.4±1,66 day. That is, the time from subclinical tracheobronchitis to clinical manifestations average of 8.0±0.33 days. In addition, in 6 (60%) of 10 cases of tracheobronchitis calves were recorded complication in the form of pneumonia.

Example 3. The rationale of efficiency.

In agriculture, stationary troubled by tracheobronchitis viral etiology (PG-3 and RTI), has selected 14 calves 1-1,5 months of age who do not respond to palpation of the last tracheal ring, but responds to intravenous administration of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution at a dose of 0.4 ml per kg of body weight of the cough response. In calves recorded time of occurrence of cough response after intravenous injection of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution, and the time of occurrence of the sensitivity of the last tracheal ring to palpation. The results are presented in table 8.

From table 8 it is seen that 50% of calves cough response was recorded 1-2,5 minutes after injection of 0.6% solution of the hydrogen peroxide 0.9% solution of sodium chloride at a dose of 0.4 ml per kg of body weight, at 28.6 per cent 3-4,5 minutes and 21,4% of the animals in 6.5-7 minutes. That is, the average time of occurrence of cough response after intravenous injection of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution was 3.5±0.56 minutes.

At 7.1% of calves sensitivity of the last tracheal ring (subclinical tracheobronchitis identified by palpation) were recorded on the 2nd day after revealing cough reaction of the inventive method, in 50% of animals for 3-4 days and 42.9% of calves in 5-6 days. That is, the use of the proposed method in comparison with the known leads before 4.1±0.36 days to diagnose subclinical tracheobronchitis.

Thus, the inflammatory process in the mucosa of the trachea and bronchi, with the active participation of AI viruses-3 and RTI increased the sensitivity of the first of the bronchial mucosa and the last tracheal rings, then the first ring of the trachea, which ultimately manifested itself a symptom of tracheobronchitis. Increased sensitivity of the mucous membrane of the trachea and bronchi intravenous administration of 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution at a dose of 0.4 ml per kg of body weight of the animal led to the Contracting of the trachea and bronchi and, as a consequence of the cough response.

Experiments indicate the feasibility of using the method that takes into account the reaction of the calves is and intravenous administration of 0.6% hydrogen peroxide solution in 0.9% solution of sodium chloride, that allows early diagnosis of tracheobronchitis and timely treatment and preventive measures.

Sources of information

1. Internal non-infectious diseases of farm animals. Ed. Igurable. Ed. 5-s Corr. and Ni. M., head, 1976. - S-140.

2. Prevention of non-communicable diseases of young animals. / Pub, Igirisu and others - M.: Agropromizdat, 1990. - C.110-115.

3. Ecological adaptation strategy to protect the health and productivity of animals in modern conditions. / Resp. edit shahs A.G. - Voronezh: Voronezh state University, 2004. - S.

4. Nachmanson V.M., Burba G. Differential diagnosis of infectious diseases of farm animals. The Handbook. - M.: Rosagropromizdat, 1990. - 225 C.

5. Viral diseases of animals. / Surin V.N., Samuyilenko YA, Soloviev BV, Fomin NV - M: UNITEMP, 1998. - 928 S.

6. Belyakov IM Diagnosis of internal non-infectious diseases of farm animals. - M.: Kolos, 1975. - 45 S.

7. Method for the diagnosis of pulmonary hypertension. RF patent for the invention №2103910, Efforescence, Navtronics.

8. The method of early diagnosis of tracheobronchitis in newborn calves. RF patent for the invention №2320254, Zolotarev A.I., shahs A.G., Turkish M.I., Twin GN.

9. Bannenberg G. Hydrogen peroxide-induced broncho - and vasoconstriction in the isolated perfused and ventilated guinea pig lung. / Banenberg G., Kimland M, Ryrfeldt A., Moldeus P. // Pharmacol. Toxicol. 1993 Apr-May; 72 (4-5): 314-320.

10. Olafsdottir K. Mechanisms of hydroperoxide-induced broncho - and vasoconstriction in isolated and perfused rat lung. / Olafsdottir, K., L. Atzori, Ryrfeldt, A., Berggren, M., Kumlin M, Moldeus P. // Pharmacol. Toxicol. 1991 Mar; 68 (3): 181-186.

The method of early diagnosis of tracheobronchitis in calves, characterized in that intravenously injected with 0.6% hydrogen peroxide solution in 0.9% sodium chloride solution at a dose of 0.4 ml per kg of body weight and, in the case of cough response through 1-7 min after injection, diagnose kennel cough.



 

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3 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to therapeutic dentistry and can be applied for treatment of apical periodontitis. For this purpose mechanical necroectomy from root canals, their mechanical cleaning and widening are carried out. As tooth canal widens multiple introduction of 5% metronidazole solution is performed. After that turunda, soaked with Polyoxydonium solution, is inserted into the canal, and left there until the next visit to the doctor. Canal orifice is covered with preparation "Sandarak Plus". After 48 hours tooth canal is opened, processed with 2% water solution of sodium hypochlorite, 5% solution of metronidazole and filled with guttapercha.

EFFECT: method ensures increase of treatment efficiency due to reduction of toxic impact of used preparations on periodontium tissues and quick elimination of pain syndrome, as well as reduction of treatment terms and simplification of treatment process.

1 ex

FIELD: medicine.

SUBSTANCE: according to the invention there is provided a spray-type nasal medicine containing active and additive agents, natural minerals and microelements, wherein natural sea-salt is used as active agent, water for injection is used as additive agent where osmolality of isotonic sterile solution makes 220 to 350 mmole/kg of water and pH from 6.0 to 9.5 and contains ions of natrium, magnesium, calcium, kalium and hydrocarbonates. The medicine is intended for active irrigation, rhinenchysis and cleansing nasal cavity, free nasal breathing management, prevention and treatment for infection of upper air passages.

EFFECT: components forming part of active agent complement and potentiate each other.

2 cl, 2 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: therapy of the patients suffering from chronic lower limb ischemia involves sampling the autogenic blood cell mass in amount 6-8% of total blood volume by discrete plasmapheresis. Then the autogenic cell mass is incubated with pentoxifylline 10 ml during 20 min at room temperature with ATP 2 ml added. The prepared mass is reinfused to the patient. Thereafter, Vasaprostan is introduced in a dose 40 mkg/day with physiologic saline 200 ml as intravenous infusion during 3 hours. Therapeutic course is 10-15 daily procedures.

EFFECT: higher clinical effectiveness with regard to the pathology due to correction of hemodynamic disorders in an ischemic focus and improvement of rheological properties blood in the absence of absolute indications to surgical procedure or inability thereof.

2 ex

FIELD: medicine.

SUBSTANCE: for preventing and treating of inflammatory processes in an animal's organism, the animal's body lesion is coated with a water-vegetative preparation, also to be introduced in a vaginal, uterine or intestinal cavity. Said preparation is made as follows. Powdered dry drug plants are placed in a dark glass bottle in a certain weight ratio. The plant mixture is covered with a vegetable oil 2500.0 g and kept at room temperature in continuous stirring for 30 days. Then a liquid component is separated to produce an oil-vegetative preparation. The water-vegetative preparation is made by covering the raw material with 2500.0 g of hot, but no more than 50°C, 0.9% sodium chloride brine, and kept in stirring for 30 days at a room temperature. Then formalin is added at 0.6 g per 100.0 g of water-vegetative infusion.

EFFECT: more effective prevention and treatment.

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to surgery and anaesthesiology, and can be used for pain management in early postoperative period following minimally invasive surgeries. Therefor, a principal stage of the limited-incision operation with use of an apparatus "Miniassistant" or a laparoscopic operation is followed by sticking a needle in a point along the abdominal midline 1-2 cm above a navel and 1 cm to the right perpendicularly to skin. The needle is penetrated therein to pierce aponeurosis of wide abdominal muscles to downfall sensation and inserted into round ligament of liver with adjusting the needle position visually. Then along the needle lumen, a conductor and a catheter are delivered through. Within the first postoperative days, exactly immediately after operations, then in 12 and 24 hours, Naropin dosed 2.5 mg/ml in 0.9% normal saline is introduced into the catheter.

EFFECT: method allows providing the most safe and effective anaesthesia with decreasing in doses of anaesthetising preparations and eliminating narcotic analgesics.

2 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, particularly to operative obstetrics, and concerns integrated prevention of purulent-septic caesarean section complications that is ensured by intraoperative regional introduction of an antibiotic. Uterine cavity and abdominal cavity are irrigated with ozonised 0.9% normal saline of ozone concentration to 4 mg/l. During two postoperative days, ozonised 0.9% normal saline of ozone concentration 1-2 mg/l is introduced intravenously drop-by-drop in amount 400 ml. Starting from the end of the postoperative day, anterior vaginal fornix is exposed to low frequency pulse magnetic field generated by a vaginal inductor introduced therein for 15 minutes daily during 5-7 days.

EFFECT: method provides accelerated regress of pain syndrome, and involution of uterus, allows reducing introduction of antibiotics.

1 dwg, 2 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: invention concerns medicine, therapy. The way includes blood sampling and its centrifugation. Then allocation of erythrocyte fraction and plasma removal is carried out. An angiotropic medicinal substance in quantity of an average therapeutic dose on each 200 ml of erythrocyte fraction is administered into the allocated fraction. Then irradiation with low-intensity helium-neon laser radiation with 1.2 mW power within 20 minutes is performed. 100 ml of a normal saline solution on each 200 ml is added in erythrocyte fraction and returned within 1.5-2 hours. The way increases efficiency of treatment at the expense of including activisation of an angiotropic preparation in erythrocyte cells at a laser irradiation of packed red cells.

EFFECT: increase of treatment efficiency at the expense of including activisation of an angiotropic preparation in erythrocyte cells at a laser irradiation of packed red cells.

2 cl

FIELD: medicine.

SUBSTANCE: invention concerns medicine, namely efferent methods of treatment. For this purpose intravenous injection of 400 ml of a 0.06% sodium hypochlorite solution with the subsequent low-flow ultrahemofiltration is performed. The way allows increasing efficiency of detoxicating of an organism at the expense of transfer of toxins from hydrophobic to hydrophilic condition.

EFFECT: rising of efficiency of detoxicating of an organism at the expense of toxins transfer from hydrophobic to hydrophylic condition.

2 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: application of 3% catolyte of a sodium chloride solution with ORP parametres minus 450-600 mV, pH - 8.2-9.5 for treatment of depressions is offered. It is shown in experiments and in clinical practice that 3% catolyte of a sodium chloride solution with ORP parametres minus 450-600 mV, pH - 8.2-9.5 possesses potentiating activity in relation to antidepressive preparations, besides, has own antidepressive effect.

EFFECT: development of an agent which possesses potentiating activity in relation to antidepressive preparations, besides, has own antidepressive effect.

2 tbl

FIELD: medicine.

SUBSTANCE: invention concerns medicine area, namely to treatment and cancer prevention. Application of nonradioactive strontium with an amino acid and an inorganic element for manufacturing of the pharmaceutical agent for treatment or prevention of cancer of prostate, carcinoma of kidneys or melanoma is offered. Application of nonradioactive strontium allows reducing cost of treatment and improving treatment acceptability by patients at the expense of absence of by-effects at application of the offered pharmaceutical agent. Also, application of the offered pharmaceutical agent allows reaching a nontoxic way of treatment and cancer prevention.

EFFECT: reduction of cost of treatment and improvement of treatment acceptability by patients at the expense of absence of by-effects at application of the offered pharmaceutical agent, and also achievement of a nontoxic way of treatment and cancer prevention.

8 cl, 3 ex, 4 tbl

FIELD: veterinary.

SUBSTANCE: method consists in obtaining complex of helminth antigens by preparing extract from separated from dead dogs male Dirofilaria immitis and adult female Dirofilaria immitis with microfilarias. After that concentration of protein in extract is brought to 15 g/l by method of dialysis and reactions of precipitation in agar gel between complex of helminth antigens and researched serum of animal are performed.

EFFECT: method allows to increase accuracy of dirophylariosis diagnostics in dogs and to reduce time of its carrying out.

1 tbl, 3 ex

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