Method of chronic disease treatment

FIELD: medicine.

SUBSTANCE: invention concerns medicine, particularly therapy, and can be applied in chronic disease treatment. Method involves causative agent extraction or determination of antibodies to causative agents or extraction of genetic components from blood, body fluid or smear. Treatment is performed by vaccine drugs specific to one or several identified causative agents, additionally immunomodulators are administered.

EFFECT: activated repair processes, arrested development of disease state of organs and systems due to elimination of identified pathogen from organism and to immune correction.

3 cl, 8 ex

 

The invention relates to medicine and can be used for the treatment and prevention of a wide range of diseases.

It is known that the chronic diseases associated with various external and internal factors[1, 2, 3].

The authors claimed the patent was found that most chronic diseases are organosedimentary Multisystem toprofessional chronic infectious process, i.e. have an infectious origin.

In the survey group (more than 300) practically healthy persons at a young age 14-23 years - found that most of them (83%) have impaired health. All persons in this group revealed the persistence of infectious pathogens.

In persons with chronic diseases of internal organs (surveyed more than 1000 patients), regardless of age, persistence of the pathogen (pathogen) was found in 100% of cases with the frequency of occurrence of specific pathogens, similar to a group of young persons.

When comparing the results of the examination of young persons and patients with chronic diseases, the conclusion follows that in the vast majority of cases of chronic diseases are caused by pathogens of viral, bacterial and fungal nature, develop slowly over several years or decades since p is a transfer from prenosological forms deployed by clinical disease.

In the majority of cases with clinical disease in the host organism revealed the persistence of the two pathogens (virus + bacteria bacteria + bacterium, virus + virus). Often a combination of virus + bacteria.

Identification of pathogens was performed in different ways:

- isolation of pathogens from the blood (plasma, serum and other biological fluids and/or seeding smear;

determination of antibodies to pathogens reaction of complement fixation or ELISA analysis;

- determine the genetic components of pathogens using polymerase chain reaction.

The causative agents of chronic diseases represent a spectrum of bacteria, viruses, fungi, etc. that collectively included in polietiologic group. Having different antigenic properties, the pathogens causing the differences in the reactions of the microorganism, especially of the immune system, connective tissue, and in dystrophic, and atrophic, regeneration, sclerotic processes. Years of research have proved the major group of pathogens are: herpes virus simple, cytomegalovirus, virus Apsta-Barr, adenovirus, influenza virus, hemolytic Streptococcus, Streptococcus viridans, Staphylococcus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, sa is monella, chlamydia, Aspergillus fumigatus, Candida albicans.

For chronic diseases of internal organs are virtually indistinguishable on clinical manifestations at different etiological basis. Therefore, the "General" approaches in the treatment of chronic diseases can not, in principle, to ensure the recovery of the body without etiological oriented treatment. It was conducted by a vaccine preparations that are specific for one or more identified agents. This treatment is complemented pathogenetic, namely the use of immunomodulators, activating innate immunity.

With this purpose used the following drugs: Imunofan, ribomunyl, Erbisol, vobenzim, immunal, Engystol.

Protecting the body from pathogens (pathogens) results in stopping the disease development of several organs and systems, caused by one common infectious cause, to the activation of reparative processes and the disappearance or reduction of painful symptoms. Cases of partial conservation of symptoms associated with advanced pathological processes in the body.

The results provide previously unknown possibilities of etiologic treatment of several chronic diseases of internal organs of the patient, caused by one common infectious cause. Removing these is a logical Alphas leads to improvement of the whole organism.

Modern medical science uncovered the subtle mechanisms of development of infectious process, including explaining the General regularities of formation of chronic infection. Chronic infectious condition caused by various disorders of immunity: the development of immunodeficiency, immunopathological and autoimmune reactions [4].

In General, the immunodeficiency can be considered as a violation of the immunological processes of regulation [4]. One of the key points of this violation is the chain of events resulting from infection in the cells of the immune system. Proven ability of viruses to multiply in B-cells, T-lymphocytes, natural killer cells and phagocytes. Apparently, under certain conditions, as targets can be a population of T-suppressor/killer cells and a subpopulation of granulocytes. The fate of infected immunocompetent cells varies. In some cases, is their elimination due to cytopathic virus activity or as a result of the destructive effect of the effector mechanisms of the immune system that can recognize these cells as foreign due to the expression on their membranes viral antigens.

Often viruses cause chronic process without morphological changes in immunocompetent cells, which are transformed into the reservoir of the pathogen is not recognizable by the system of immunological surveillance. Most often, the persistence of the virus develops in the cells phagocytes. In chronic infection are formed functional defects of immune cells, as a rule, are affected, not all but only some properties, but it is sufficient for chronic persistence of the pathogen in the body.

Secondary immunodeficiencies are recognized as an important element in the pathogenesis of viral infections. Associated with the development of acceding diseases, the pathogens which are the different classes of pathogens: bacteria, viruses, fungi and parasites. Often these affiliate infection define the clinical picture of the final stage of the underlying disease and its outcome. Experiments with flaviviruses demonstrated that secondary immunodeficiency in some cases determine the clinical manifestation heterologous infection of viral etiology, while others lead to the development of the second persistence of the pathogen in the brain cells or thymus [5]. In this light, it becomes obvious great danger of mixed viral infections compared with monoinfected.

Immunosupressives activity of viruses in most cases does not correlate with virulence. For example, balapatabendi virus Tahina in mice causes a more pronounced immunologicals the e defects, than the virus Langat. It is sequential infection called pathogens is formed of chronic virus infection Langat Central nervous system, which is characterized by failure of the process of perception and information processing.

In 80-ies received General recognition of the concept of two sides of any, including antiviral immune response. According to this concept, the immune response provides for elimination from the body of infectious agents, at the same time causes damage to uninfected cells, tissues and organs of the host due to generation of immunopathological antibodies [4, 5]. In General, we can conclude that harmless immune reactions does not exist [6, 7].

The development of viral infections of humans and animals is always accompanied by the formation of autoimmune reactions [5]. Autoimmune response observed in acute and asymptomatic infections, infections that are limited in time, and infections, which lasts for several months or years.

Formed in response to antigens of the pathogen antibodies react with antigens on cell structure and connective tissue of the host.

At the end of XX century it became apparent that, virusinduced autoimmune process plays a significant role in the formation of a wide range of clinically significant symptoms and functional impairments that define the degree or another picture of the underlying disease.

Data is available on the relationship of persistent measles infection with systemic lupus erythematosus, systemic scleroderma [8].

Discussed and proved the involvement of autoimmunity in the development of functional disorders of various populations of immune cells, including immunoregulatory, for example, T-helper cells.

It is recognized the value of an autoimmune response in established autoimmune complexes and occurs associated with the deposition of these complexes in the pathology of many organs. Seems legitimate to conclude that the basis of any arising in viral infections and immunological mediated damaging reactions can be the activity of autoantibodies and autoimmune T lymphocytes.

For the emergence of infectious diseases of bacterial pathogens must first open physical barriers, such as mucous membranes, and then to freely breed with a strong dissemination, avoiding destruction by the immune system. Many bacterial pathogens have such properties due to secretion of the protein in the host organism, which play the role of a destroyer or damper in relation to a massive immune response [9].

Bacterial pathogens use the Arsenal of secreted virulence proteins for modification of the innate the immune activation by changing the recognition by the immune system microbial implementation.

For example, the bacterium injects effector proteins into the host cell, leading to active blocking of phagocytosis, and modifies other adequate reaction [10].

Another option of avoiding the immune response of the host is as follows. The cytoplasm of the host cell uses protein receptors, called NOD proteins [11]. NOD receptors participate in the identification of the composition contained in the bacterial cell.

Functional value alarm NOD can be strengthened by identifying a patient with NOD-mutation, which is more susceptible to chronic diseases of bacterial etiology; disease - associated mutagenic changes receptors are not able to recognize peptidoglycan. Now described is evidence that proteins of bacteria serves cascade antagonistic signals directly address NOD.

However, modification or damping NOD - dependent sequential signals may allow intracellular pathogens hidden to penetrate into the host cells, allowing them to evade detection and to minimize the Pro-inflammatory response, which was filed would alarm the immune system to the presence of the pathogen [10].

The past decades have witnessed a rapid growth of scientific achievements in two related areas: innate immunity and microbial shall Athenes. Many pathogens have mechanisms of variability to infect their host under conditions of a fully functioning innate immune system, and at the same time, there are many examples in which pathogens avoid detection and/or suppression of inflammation. There is a hypothesis that pathogens not only survive if the reactions of innate immunity, but also use it to increase their pathogenicity [10].

From these data suggest that currently uncovered new mechanisms of development of infectious process, including explaining the General regularities of formation of chronic diseases or primarily associated with a specific pathogen or associated with the pathogen, which in some cases mainly determines the course of the clinical picture and outcome of the disease.

Virusinduced immunosuppression is associated with increased sensitivity to heterologous pathogen. The described phenomenon of combining weak immunomodulatory signals subparagraph doses of virus and immune modulators, resulting in the development of severe immunosuppression. This may explain the pronounced co-existence in the microorganism of two or more agents.

In the process of pathogenesis of viral infections can join the causative agents of different classes: bacteria, is irony, fungi, parasites, which often determines the clinical picture of the final part of chronic disease and its outcome. Frequent is the weighting painful process, modified heterologous pathogen. This obvious is a great danger of mixed infections compared with monoinfected. This is especially important because it is known that subsequent infection of other balapatabendi viruses pathogenicity one of them can dramatically increase with damage to vital systems.

Finding care microorganisms from the immune response of the host, development of the latent, indolent infection with the gradual defeat of a number of organs and systems, resulting in the development of various chronic diseases, traditionally regarded as independent from each other. In these cases, treatment is not aimed at the elimination of the etiological factor, the result is impossible to achieve radical cure of the body.

On the basis of own and literature data, we have proposed and studied the assumption and proved his justice, according to which the treatment of chronic somatic pathology should be integrated and combined impact on the etiological agent, and running them pathological processes. On financial p the results of our work impact on the pathogen must be twofold: the use of specific vaccines, if the pathogen is known, and the activation of innate immune recognition and elimination of both known and unknown pathogen.

Such treatment using high-performance antipathogenic media can provide and the elimination of immunopathological antiviral antibodies responsible for damage to uninfected cells, tissues, organs of the host, since these antibodies bear the internal image of the pathogen.

Thus, effective treatment of chronic diseases is possible only with the observance of certain rules.

1. To identify infectious pathogen (virus, bacteria, fungal infection, etc. with their types and possible combinations).

2. Selection of homologous vaccines that can protect the body against the identified pathogen (pathogen).

3. To carry out the activation of innate immunity drugs, bearing ligands for receptors of the immune system in order to eliminate immunodeficiency, immunopathological, autoimmune conditions, and damage to the host cells.

Repeat performance measures position 1 after treatment to confirm the elimination of the pathogen from the body.

Using the described method of treatment of chronic diseases with the body's defense against pathogen and normalization functions, immunity is that leads to reverse the development of pathological processes and disappearance or significant reduction of painful symptoms.

Example No. 1.

Patient K., born in 1989, complained of headaches and sleep disorders with 3 years of age. Over the past period has been repeatedly examined and periodically passed courses of treatment with a neurologist and therapist. Treatment was conducted: bellaid, nootropil, trental, acid, Cavinton, phenazepam, Cinnarizine. The effect of treatment was observed. When our immunological examination revealed antibodies to the herpes virus simple. Received 2 cycles of treatment herpes vaccine at a dose of 0.3 ml/m 5 injections per course with a 2-week break between cycles. For normalization of innate immunity was assigned a dose of 2 ml/m for 20 days.

Currently headaches do not worry, sleep is normalized. The observation period of four years.

Example No. 2.

Patient K., born in 1954, had suffered from bronchial asthma for 15 years. The last few years constantly applied aerosol broncholytic (salbutamol, fenoterol). Asthma attacks continued. For our examination in culture revealed Staphylococcus aureus. Treatment vaccine staphyloccocus course in 20 injections every 3 days with increasing doses from 0.1 ml to 1 ml For normalization of innate immunity were administered Wobenzym 3 tablets x 3 times per day for 2 weeks.

Currently, asthma, presupuesto.

The observation period of three years.

Example No. 3.

Patient M., born in 1965, complained of intermittent arrhythmia within the last ten years. Repeatedly appealed to the experts, receive quality medical care. Electrocardiographically was diagnosed with atrial fibrillation, predicitions form. Conducted by propranolol treatment were cut short by the attacks of arrhythmia, but long-term remission does not occur. When our immunological examination revealed antibodies to Pseudomonas aeruginosa. Treatment vaccine polyvalent Pseudomonas aeruginosa in 1 ml × 2 times a week, for a treatment course of 10 injections. For normalization of innate immunity were used immunol 1 tablet (80 mg) × 3 times a day for 8 weeks.

Currently, no complaints. Bouts of arrhythmia was not renewed.

The observation of three and a half years.

Example No. 4.

Patient S., born in 1972, considers himself ill over the last three years, when there is an eczematous rash on the skin. Conducted courses of treatment (Lorenzen C), a significant effect was not observed. When crops smears from the nasopharynx and the scrapings of the affected epidermis revealed the growth of fungus Aspergillus fumigatus. Treatment vaccine Aspergillus fumigatus to 3 pills x 2 times a day for 2 months. For normalization of congenital and is of immunity was appointed Engystol 1 tablet x 2 times a day for 4 weeks.

Currently, skin rash no, no complains.

The observation year and a half.

Example No. 5.

Patient R., born in 1967, complained of headaches associated with periodic increases in blood pressure (up to 160 110 mm Hg), pain in the stomach, associated with food intake, and pain in the spine in the last five years. Repeatedly addressed to specialists, received treatment: Prestarium, amlodipine, Concor, omez, diclofenac. The effect of treatment was observed. When our immunological examination revealed high titers of antibodies to the hemolytic Streptococcus. Treatment vaccine stafilo-streptococcal course in 20 injections/m every three days with increasing doses from 0.1 ml to 1 ml, and the drug - Echinacea compositum 1 ml × 2 times a week/m on a course of 10 injections. For normalization of innate immunity was administered immunol 1 tablet (80 mg) × 3 times a day for 8 weeks.

Currently, no complaints. Objectively - organ pathology is not detected, the blood pressure was stabilized at the level of 120/80 mm Hg

The observation of two and a half years.

Example No. 6.

Patient A., born in 1947, during the 2 years was observed regarding essential itching of the vulva. Treatment: creams with novocaine, glucocorticoid drugs with estrogen with testosterone, ultrasound and laser therapy, yielded no result. Immunological examination revealed antibody hemolytic Streptococcus. The treatment stafilo-streptococcal vaccine rate in 20 injections/m every three days with increasing doses from 0.1 ml to 1 ml and Imunofan 1 injection/m (50 µg/ml)×1 time in three days No. 10.

Itching of the vulva disappeared.

The observation period of 1 year.

Example No. 7.

Patient N., born in 1950, considers himself sick the last 7 years, when we began to see the pain in the calf muscles when walking, the last time the patient often had to stop and stand up to the disappearance of pain. The disruption of blood flow in the vessels of the lower extremities was confirmed by deperately. Immunological examination revealed persistence of the herpes virus simple and Streptococcus viridans. Received 2 cycles of treatment herpes vaccine at a dose of 0.3 ml/m 5 injections per course with a 2-week break between cycles and vaccine stafilo-streptococcal course in 20 injections/m every three days with increasing doses from 0.1 ml to 1 ml For normalization of innate immunity were appointed Imunofan 1 injection/m (50 µg/ml) × 1 time in three days No. 10.

After treatment, pain disappeared, which is confirmed by the significant improvement in doplersonography vessels of the lower horse the values.

The observation period of 3 years.

Example No. 8.

Patient G., born in 1972, has complained of recurrent pain in the lower abdomen. Urological and ultrasound diagnosed with chronic prostatitis. After the last exacerbation within 6 months consistently were treated with antibiotics (Hemomycin, Amoxiclav), magneto-laser therapy (10 sessions). The effect of treatment was observed. Immunological examination revealed Staphylococcus aureus. Treatment vaccine staphyloccocus course in 20 injections every 3 days with increasing doses from 0.1 ml to 1 ml and Imunofan 1 injection/m (50 µg/ml) × 1 time in three days No. 10.

The symptoms disappeared.

The observation period of 1.5 years.

Literature

1. The practitioner's Handbook / edited Aiguablava - M.: Publishing house Eksmo, 2005. - 1200 C.

2. Soludo B. I., Makarenko ST. Standards for the diagnosis and treatment of internal diseases. 3rd ed. - SPb.: "ELBI-With-P, 2005. - 800 C., Il.

3. Dillman V.M. Four models of medicine. - L.: Medicine, 1987. - 288 S., Il.

4. Semenov B.F., Harenski CENTURIES of Autoimmunity in viral infections. The results of science and technology. - M., 1993, t, 152 S.

5. Semenov B.F., Vargen CENTURIES Immunomodulation in viral infections and vaccination. The results of science and technology. M., 1988, V.17, 161 S.

6. Zverev V.V., Markusen YEAR, Uminova NV Measles. Molecular genetics of wosb the producer, epidemiology, specific prevention. Edited by Zverev V.V. S.-Pb., ed. St. Petersburg state University, 2004, 110 S.

7. Faizullaev E.B., Nikonov A.A., Oksanych A.S., Borisenko A.S., Zverev V.V. Development of a PCR system for detection of adenovirus infections in humans // Virology. - M., 2005, No. 6, p.44-47.

8. The Yuminov N.V., his living V.M., Alexander S. Kaliev, Zverev V.V. Measles infection: stage elimination. pp.163-167.

9. Coombes VK, Valdez y, Finlay CENTURIES Evasive Maneuvers by Sekreted Bacterial Proteins to Avoil Innate Immune Responces. Current Biology. Vol.14, R 856-R 867. Oktober 5, 2004.

10. Inohara N, Nunez G: Intracellular proteins involved in inflammation and apoptosis. Nat Rev Immunol 2003, 3:371-382.

11. Porthoy D.A. Manipulation oxinnate immunity by bakterial pathogens. Current Opinion in Immunology, 2005, 17:25-28.

1. A method of treating chronic diseases other than infectious, consisting in the selection of pathogens or the detection of antibodies to pathogens or their genetic components from a number of the herpes virus simple, cytomegalovirus, virus, Epstein-Barr, adenovirus, influenza virus, hemolytic Streptococcus, Streptococcus viridans, Staphylococcus, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella, chlamydia, Aspergillus fumigatus, Candida albicans, and the treatment of vaccine preparations specific for one or more identified agents.

2. The method according to claim 1, characterized in that for bacteriological and/or immunological analysis using cu is V, and/or biological fluids, and/or stroke.

3. The method according to claim 1, characterized in that the conduct additional treatment with immunomodulators.



 

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14 cl, 1 dwg, 1 tbl, 2 ex

FIELD: biotechnology, immunology, veterinary science.

SUBSTANCE: invention proposes the preparation that represents 9,0-11.0% immunoglobulins solution of horse blood serum with pH value from 7.0 to 7.5. The content of γ-globulin fraction in this preparation is 90.0%, not less, of the total protein amount. The preparation comprises albumin, α- and β-globulin fractions as nonspecific impurities in the amount 10.0%, not above, and residual ethyl alcohol in the amount 4.0%, not above. The preparation is apyrogenic, non-toxic and sterile.

EFFECT: valuable properties of preparation.

1 tbl, 1 ex

FIELD: medicine, veterinary science.

SUBSTANCE: group of inventions concerns veterinary science and biotechnology area. The way of reception of a brucellous antigen from the Brucella abortus 19 strain for manufacturing of a uniform brucellous antigen for AA, CFR and LCFR includes reception of an inoculum and propagation of brucellas in deep conditions on a liquid nutrient medium with regulation of level of partial pressure of oxygen dissolved in a culture liquid throughout all propagation process, separation and concentration of evolved bacteriemic cells using ultrafiltration and their inactivation using warming. Thus brucellas propagation in deep conditions on a liquid nutrient medium with regulation of level of partial pressure of oxygen dissolved in a culture liquid throughout all propagation process is performed in a following regimen: temperature 37±0.5°C, pH 6.9-7.2 aeration from 1 hour till 3 hour - 20-25 pO2, from 3 hour till 6 hour - 25-40 pO2, from 6 hour till 15 hour - 40-45 pO2 and from 15 hour till 16-18 hour - 30-35 pO2. Separation and concentration of the evolved bacteriemic cells by ultrafiltration is carried out with use of membranous cartridges. The concentrate of an antigen received by given way is used also for reception of an antigen for Rose Bengal Assay (RBA), for reception of a brucellous antigen for ring test (RT) with milk and reception of diagnostic brucellous serum which, together with blood serum of healthy livestock, make a part of sets for brucellosis diagnostics. The group of inventions allows to raise efficiency of process of brucellosis diagnostics and to improve control at serologic examination of animals.

EFFECT: rising of efficiency of process of brucellosis diagnostics and improvement of control at serologic examination of animals

22 cl, 11 ex

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