Method of neoadjuvant chemotherapy of invasive bladder cancer
SUBSTANCE: invention refers to medicine and namely to oncourology and can be used for treatment of bladder tumours. The method is implemented in the following way: during the period prior to the operation there created is depot of cytostatic incubated with autoplasma in regional lymphatic nodes. For that purpose 50 ml of blood is taken from the patient, centrifugated at the speed of 1500 rpm during 40 min; 15 ml of the obtained autoplasma is added to cytostatic diluted in the proportion of 0.9% NaCl per 5 ml, and incubated during 1 hour at t=37°C. Then puncture of lymphatic vessel on the back of the foot is performed and cytostatic with autoplasma is injected; repeated injection in one and the same limb is carried out in 12 days.
EFFECT: use of invention provides the effect on tumour and its regional metastases owing to keeping the remedy in lymphatic bed for a long time and its being deposited in regional lymphatic nodes at low probability of complications.
The invention relates to medicine, in particular to oncourology, and can be used in the treatment of patients with malignant tumor of the bladder at II-III stages of neoplastic process.
The known method chemotherapeutic treatment of invasive bladder cancer (see the book "Clinical oncourology" /edited by Matveeva BP - M.: 2003, s-368; guidelines for chemotherapy of neoplastic diseases /edited Perevozchikova NI - M, 2005) which consists in the application schema M-VAC (Methotrexate 30 mg/m2in/in 1 day, Vinblastine 3 mg/m2/the 2nd day, Doxorubicin (Adriamycin) - 30 mg/m2in the 2nd day, Cisplatin 70 mg/m2/drip in the 2nd day. Dose methotrexate and vinblastine repeat on the 15th and 22nd day when normal blood counts. A second course in 4 weeks).
Systemic polychemotherapy has a number of side effects due to its toxicity: complications of the cardiovascular system, the gastrointestinal tract, the blood-clotting system, immune system, which leads to interruption of specific treatment.
Known "Method of treatment of bladder cancer by autohemotherapy" (see Nazarov O.E. Berlov Autohemotherapy locally advanced bladder cancer: author. dis. Kida. the honey. Sciences, R-n/A. 2003). The way VC is uchet introduction patient anticancer chemotherapy sredneterapevticheskih doses:
cyclophosphamide, doxorubicin, cisplatin, diluted to 150 ml of autologous blood taken from a patient from a vein in 2 sterile vial with 50 ml of pulizia. Vials administered drugs on physiological solution. The composition is incubated for 30 minutes at t°=36,5 is 37.0°C in termostate followed by reinfusing autologous blood with cytotoxic drugs to the patient/drip under conditions of overload. The method is effective, but has drawbacks: accompanied by toxicity, no effect on the lymphatic system.
Known "Method endolymphatic chemotherapy" (see the book "the Endolymphatic chemotherapy in the clinic", Ed. Sidorenko US, Rostov-on-don, 1998, p.120), chosen as a prototype. The way endolymphatic chemotherapy of bladder cancer was used for functional contraindications to surgery and radiation treatment in the neoadjuvant mode. The results of chemotherapy (at the rate of 2000 mg of cyclophosphamide and 2000 mg of 5-fluorouracil or 100-150 mg Thiotepa) was measured at the improvement of the General condition General condition, decrease in dysuria and hematuria. However, the known method has drawbacks: endolymphatic administration of chemotherapeutic agents for the impact on the entire regional lymph collector affects the change of peripheral blood.
After infuse is a decrease in the number of cells compared to baseline levels. A slight decrease toxicity does not allow polychemotherapy in full. Changing parameters of peripheral blood after APT is of interest for further clinical research in this field.
The aim of the invention is the effect on the bladder tumor and regional lymph nodes, decrease complications of chemotherapy.
This objective is achieved in that in the preoperative period create a depot of chemical, incubated with autoplasma in regional lymph nodes: taking 50 ml of the patient's blood, centrifuged with a speed of 1500 rpm for 40 min, the resulting autoplasma in the volume of 15 ml combined with cytostatic agents (cyclophosphamide 2000 mg), diluted to 5.0 ml with 0.9% NaCl and incubated at t=37°C for 1 hour, then perform a lymph vessel on the rear foot and injected chemotherapy, incubated with autoplasma, following the introduction of the cytostatic agent in the lymphatic vessels of the same legs can be performed after 10 days, the vessels of the opposite legs after 6-7 days.
The invention "Method of neoadjuvant chemotherapy in invasive bladder cancer" is new, because it is not known from the level of medicine in the treatment of patients with malignant tumor of the bladder at II-III stages of neoplastic process.
The novelty is subramania is in the preoperative period create depot anticancer chemotherapy on autoplasma patients with bladder cancer stage II-III tumor process, has never been applied in medical practice in malignant tumors of the urinary bladder.
Due to the lack of selectivity existing anticancer drugs damage the tumor cells, and normal, fast-growing cells, the first cells in the bone marrow. With increasing total dose of cytostatic along with increase the antitumor effect usually develops oppression of the blood forming organs. Although endolymphatic chemotherapy allows you to enter massive doses of chemotherapy, this technique does not lead to a significant reduction of toxicity of the latter. Changes of parameters of peripheral blood after APT of great interest, because it is possible to judge the degree of toxic effect on the organism massive doses of chemotherapy, administered endolymphatic.
Endolymphatic autolackiererei is a perspective neoadjuvant treatment of malignant tumors of the urinary bladder in II-III stages of the process. The route of administration of cytostatic agents on autoplasma helps to improve the selectivity and efficiency of cancer treatment.p> The method makes it possible to greatly reduce the frequency, severity and duration of toxic reactions to avoid some complications, limiting the use of systemic chemotherapy.
Achieved in the short term regression of the primary lesion and metastatically modified lymph nodes contributes to the further successful technical operation.
The invention "Method of neoadjuvant chemotherapy in invasive bladder cancer is industrially applicable, as it can be reproduced in health care, cancer research institutes, breast care clinics and other medical institutions for the treatment of patients with bladder cancer.
"The way neoadjuvant chemotherapy in invasive bladder cancer" as follows: by puncture of peripheral veins is the fence 50 ml of the patient's blood into a sterile vial with 10 ml of Pulizia, followed by centrifugation of the blood (speed 1500 rpm) for 40 minutes thus Obtained plasma of the patient is connected in a volume of 15 ml autoplasma with cytostatic agents (Cyclophosphamide 2000 mg, dissolved in 5 ml of 0.9% NaCl) and incubated at t=37°C for 1 hour. Then the corresponding method is performed lymph vessel on the rear foot, the latter shall provide for the introduction of chemical, incubated with patient plasma. Re-introduction of the cytostatic agent is 12 days. Immediately before the operation is performed assessment objective status of the patient (assessment of patient complaints and the severity of clinical manifestations, ultrasound of the bladder to assess tumor process in the dynamics). In the postoperative period is estimated degree of medical pathomorphosis of the tumor and changes in regional lymph nodes.
An example of a specific implementation of the Method neoadjuvant chemotherapy infiltrative bladder cancer".
Patient K., 71, medical card number C-2340/h, was admitted to the Department of oncourology 18.02.08 with complaints of difficulty, painful, frequent, mocheispuskanie, periodically-makrogematuriya(last 8.01.08) According to a survey (ultrasound from 21.01.08 - on the right side wall of the bladder lazerous hyperechoic education size of 0.8×2.3 cm) including ROUND-biopsy of the bladder performed at the place of residence of the patient (the state institution "Regional clinical oncological dispensary health Department of the Krasnodar region), GA. No. 106115-116 - moderately differentiated perekhodnocletocny cancer with invasion to the muscle layer, diagnosed with bladder cancer T2NoMo, CL gr. 2. Cystoscopy (11.10.07, epicrisis No. 07.2123 from 16.10.07), made in goose "Regional clinical oncological dispensary health Department of the Krasnodar region - 1-3 segments on the back wall with the transition to the right side wall several merging between a villous tumor sizes ranging from 1.5 to 3.0, see the bladder neck enlarged adenomatous nodes, on which surface a circular tumor growth. Right in the mouth area of the tumor, left outside the mouth area of the tumor, peristaltic.
19.02.08 - made right endolymphatic infusion of 2000 mg of Cyclophosphamide, incubated with 15 ml of patient plasma. On day 5 showed reduction of dysuria. 3.03.08 (12 days) - re endolymphatic introduction of cytostatics in the right lower limb. According to laboratory research (OAK, biochemical analysis of blood, leukocyte count, urea, creatinine during chemotherapy within normal limits. Adverse reactions gastro-intestinal tract, cardiovascular system, blood is not revealed
11.03.08 control ultrasound, according to which at the base of the bladder to the right lazerette hyperechoic education without a clear path, size 0,8 see 12.03.08 the operation - ROUND-vaporization of the bladder (2-3 S on the right side wall, above the mouth of the right ureter 1 cm detected 4 is castka modified mucous, 1×1.5 cm each, suspicious tumors. 2-3 S left similar plots, a total size of 1×1.5 cm In the postoperative period without complications. The patient was discharged with adequate urination, dysuria no.
Technical and economic efficiency "Method neoadjuvant chemotherapy in invasive bladder cancer is that:
The cytostatic entered endolymphatic on autoplasma, provides not only long-term presence of the drug in the lymph channel (due to its Deposit in the regional lymph nodes, as well as the plasma protein binding), but the effect on the tumor and its regional metastases.
- Reducing the likelihood of complications conducted endolymphatic chemotherapy compared with systemic chemotherapy.
- improving the patient's quality of life by maintaining adequate functioning of the bladder
- increases the duration of recurrence-free period.
The way neoadjuvant chemotherapy in bladder cancer, characterized in that in the preoperative period create depot cytostatic, incubated with autoplasma in regional lymph nodes: taking 50 ml of the patient's blood, centrifuged with a speed of 1500 rpm for 40 min, 15 ml of the obtained autoplasma connect with the cytostatic agent is m, diluted to 5 ml with 0.9% NaCl and incubated at t=37°C for 1 h, carry the lymph vessel on the rear foot and enter the cytostatic with autoplasma, repeated administration of the same limb exercise 12 days.
SUBSTANCE: anti-cancer remedy obtaining method is based on coordination compound of bivalent platinum and in accordance with which the water solution containing humic substances is processed with the above platinum compound. As coordination platinum compounds, the compound of the following row is chosen: potassium tetrachloroplatinate, cis-dichlorodiamine platinum, hydrogen tetrachloroplatinate, potassium tetrabromoplatinate. Processing is performed under influence of wave radiation up to the value of content of high-molecular fraction of humic substances of not more than 5%. As wave radiation, there chosen is radiation in ultrasonic range of frequencies of 18 kHz to 66 kHz with power of 0.5 to 5 W/cm3. When radiation power is 5 W/cm3 and frequency is 22 kHz, processing is performed during 5-20 min. As wave radiation, there can be chosen radiation in microwave range of frequencies of 30 to 0.3 GHz and power of 0.5 to 50 W/cm3. When microwave radiation power is 0.5 W/cm3 and frequency is 2.45 GHz, the temperature of the processed product is maintained within 40-50°C during 30-90 min.
EFFECT: method provides obtaining more than one homogeneous product which is more stable during storage by decreasing relative content of high-molecular fraction.
6 cl, 5 ex, 5 tbl, 2 dwg
SUBSTANCE: there are disclosed polypeptide variants containing Fc-areas IgG, having amino acid modifications providing changed effector functions Fc in specified polypeptides. There is disclosed composition for antibody targeting on antigen, containing the specified polypeptide. There is described method for preparing the specified polypeptide. Also, there are disclosed the methods for treating V-cell tumour or a malignant disease characterised by V-cell expression of CD20, treating chronic lymphocytic leukosis, relieving the symptoms of the V-cell controlled autoimmune disease, treating a angiogenesis-associated disorder, treating HER2-expressing cancer, treating LFA-1-mediated involvement, treating IgE-mediated involvement wherein specified methods imply introduction to the patient of the therapeutically effective amount of said polypeptide.
EFFECT: higher clinical effectiveness.
63 cl, 6 ex, 13 dwg, 10 tbl
SUBSTANCE: invention refers to new compounds of formula I where R1 stands for phenyl; G stands for C1-C7-alkylene; Q stands for -NH-; and X stands for C1-C7- alkylene, or to its salts. In addition, the invention concerns a pharmaceutical composition, to application of compound of formula I as defined in claims 1-5 item, as well as the method for making the compound of formula I.
EFFECT: production of the new biologically active compounds inhibiting protein tyrosine kinase.
8 cl, 3 ex
SUBSTANCE: invention relates to group of the new compounds presented by structural formulas A-K ,
and their pharmaceutically acceptable isomers, salts, salvates and to polymorphous forms.
EFFECT: inhibitory action with respect to thrombin receptors.
20 cl, 1 tbl, 2 ex
SUBSTANCE: new 5-sulphanyl-4H-1,2,4-triazole derivatives of general formula I (meaning of radicals R1-R3 are indicated in the description of the invention), methods of their preparation by liquid-phase parallel synthesis and pharmaceutical composition are claimed.
EFFECT: claimed compounds display high affinity to some subtypes of somostatin receptors of the SST2 and SST5 subtypes and possibility of their usage for treatment of pathological states or diseases involving one or more of the given somostatin receptors
9 cl, 708 ex
SUBSTANCE: in formulas (I) and (Ia) . A is 1-cyclopentene-1,2-diyl; Z1 and Z2 are O; E is phenyl substituted with 1-4 substituting groups selected from halogen, Y is phenyl substituted with 1-2 substituting groups selected from halogen, C1-C6alcoxy, halogen C1-C6alcoxy; the meaning of other radicals are indicated in the formula of the invention. The invention refers also to the pharmaceutical composition including the compound of the invention, to the application of the said compound at preparation of the medicinal agent and to the application for DHODH inhibition.
EFFECT: compound of the present invention can find application as medicinal agent inhibiting dehydroorotate dehydrogenase.
9 cl, 18 ex
SUBSTANCE: therapy of rectal cancer is ensured by preoperative beam therapy "РОД" 5 Gy during 5 days, in combination with local microwave hyperthermia at temperature 42.5-43°C during 60 minutes for 3, 4 and 5 days after beam therapy sessions. A composite mixture containing sodium alginate, 2% dimethyl sulfoxide and 5-fluorouracil is introduced through rectum at exposition 6 hours in 1, 2 and 4 days prior to beam therapy sessions. For 3 and 5 days, at the same exposition, said composite mixture containing metronidazole instead of 5-fluorouracil is introduced. Further, surgical procedure follows.
EFFECT: due to selected introduction regiment of chemotherapeutical preparations as parts of the composite mixture, the method provides high concentration thereof in rectal tumours without side effect, ensures high degree of beam pathomorphism in vast majority of the patients of said category, the absence of recurrent tumour and dissemination within 3 postoperative years.
SUBSTANCE: treatment of benign prostate hyperplasia is ensured by oral introduction of a biologically active food additive "Photostim" as a photosensitiser within the first 10 therapeutic days in a dose 2.5 ml. Further, "Photostim" is dosed 5 ml once a day. The whole therapeutic course implies introduction of 100 ml. Starting from the 7-8 therapeutic day, suprapubic region and perineum are exposed to laser radiation of wave length 665-670 nm, power density 100 J/cm2 in continuous radiation condition, by noninvasive transcutaneous action of two fields, 20 minutes on each area as specified. Therapeutic course includes15 daily sessions. A monochromatic light source is a photodynamic therapy complex that comprises high-light semiconductor light-emitting diodes "ELAN" of array radiation wavelength 665-670 nm, in continuous radiation conditions, power density of working array radiation in focal plane 30-60 mWt/cm2 and irradiation spot area in focal plane 40 cm2.
EFFECT: reduction of recovery period, ensured absence of intraoperative injuries, prevention of postoperative complications and cicatricial changes.
2 cl, 1 ex
SUBSTANCE: pharmaceutical composition for making a parenteral preparation contains a pharmaceutical agent, a water-soluble polymer, a nonpolymeric additive and a solvent. The water-soluble polymer is chosen from the group: dextran, gelatin, 2-hydroxyethy starch, poly(N-vinylpyrrolidone), human blood serum albumin or their mixture. The nonpolymeric additive is chosen from the group: (C10-C18)acyl-sorbite, oligooxyethylene(C10-C18)acyl-sorbite, (C10-C18)alkyl oligooxyethylene glycol ester. The solvent represents mixed water and ethanol, or dimethyl sulphoxide, or N-methyl-2-pyrrolidone, or 2-pyrrolidone. The pharmaceutical agent, said water-soluble polymer, nonpolymeric additive and residual solvent are found in the preparation as a noncovalent complex.
EFFECT: improved bioavailability of the pharmaceutical agents and therapeutic dose decline.
2 cl, 1 dwg, 1 tbl, 1 ex
SUBSTANCE: invention relates to Bcl-2 proteins, fragments thereof, and to application thereof in patients with a malignant tumour. The declared proteins and peptide fragments particularly are applicable in vaccine compositions for treatment of malignant tumour. Besides, the invention concerns the methods of treatment with application of specified compositions. Also, an aspect of the invention is production of T-cells and receptors thereof which are specifically recognise declared proteins and peptide fragments.
EFFECT: higher clinical effectiveness with respect to tumours.
61 cl, 5 ex, 2 tbl, 12 dwg
SUBSTANCE: method for recovering and purifying trophoblastic beta-1-glycoprotein (TBG) from retroplacental blood serum by ammonium sulphate fractionation, washing and centrifugation of the solution residual matter, affinity sepharose chromatography followed by elution and lyophilisation of an end product. The retroplacental blood is centrifugated at 10000 rpm within one hour. Sodium chloride is added to a supernatant to end concentration 0.1-1.0 mol/l, while triton X-100 is added to end concentration 0.01-0.9%. Centrifugation is performed once again. The prepared serum is saturated with ammonium sulphate to 30%, incubated within a night in stirring, centrifugated. The deposit is threefold washed in 30% ammonium sulphate, suspended in 0.15M phosphate buffered saline pH 7.0-7.5, containing 0.5 M sodium chloride and 0.05% triton X-100, dialysed in an ultrafiltration cell, with tenfold amount of the buffered saline passed, five-fold diluted and ultrafiltered at filtration limit 100000 D. The prepared permeate is processed with single affinity sepharose chromatography with immobilised monoclonal TBG antibodies. The affinity-bound TBG is eluted with 0.1M glycine-Hcl buffer solution pH 2.55. The eluate is immediately neutralised, concentrated, dialysed against physiological saline and lyophilised.
EFFECT: higher yield and purity of the end product.
2 ex, 1 tbl
SUBSTANCE: invention refers to ophthalmology and can be used in surgery of subretinal haemorrhages combined with age-specific macular dystrophy with subretinal neovascular membrane. The technique involves subtotal vitrectomy and removal of posterior hyaloid membrane. Bevacizumab and Ranibizumab are injected intravitreally. It is followed with intravitreal injection of tissue plasminogen activator and expanding gas while a patient is pronated.
EFFECT: method allows deploying blood from under retina, ensuring pathogenetic action on haemorrhage source, preventing proliferative processes in vitreal cavity.
SUBSTANCE: group of inventions concerns biology and medicine. There is disclosed method of biotreatment blood serum production involving electrostimulation of an animal, other than a human, blood drawing of specified animal, serum separation from specified blood and gamma irradiation in a dose 10 to 40 kGy. There is offered blood serum product and pharmaceutical composition containing said blood serum product, and also their application for treatment of various diseases.
EFFECT: invention provides production of biotreatment animal's blood serum applied for treatment of wide range of diseases, including epileptic attacks and apoplexy.
24 cl, 4 ex, 7 tbl, 11 dwg
SUBSTANCE: virus-inactivated blood plasma for introduction to the person, in essence, does not contain the agents inactivating viruses, received from donors from whom 10 % or more belong to population of the non-Caucasoid race individuals, thus plasma is received by mixing of blood or blood plasma of A and B groups, it is unessential AB, without adding blood or blood plasmas of the 0 group, and is characterised that it contains: from five to six parts of blood or a blood plasma of the donors having A group of blood, from four to five parts of blood or a blood plasma of the donors having B group of blood, from zero to one part of blood or a blood plasma of the donors having AB group of blood. The pharmaceutical composition for manufacture of a medical product for treatment of deficiencies of factors of coagulation, thrombocytopenic purpura is offered, and for use in a repeated exchange of great volume of plasma. Application a virus-inactivated blood plasma according to the invention for manufacture of a medical product and the way of a virus-inactivated blood plasma manufacture is offered.
EFFECT: rising of efficiency of treatment.
10 cl, 2 ex
SUBSTANCE: in 1st, 9th, 17th and 24th days of therapy, 5-fluorouracil 750 mg and cisplatin 30 mg incubated with autoblood are introduced intravenously. In 7th, 15th and 23rd days, cisplatin 10 mg incubated with autoplasma, Novocaine 0.5% 20 ml, hydrocortisone 1.0 ml, analginum 50% 2.0 ml, vitamin B12 1.0 ml and gentamycin 1.6 g are introduced into presacral of small pelvis. From 10th to 14th days, 2 hours prior to radiotherapy session, a mixture containing Doxorubicin-Ebewe 5 mg, Viferon ointment 2.5 mg, Vinilin 2 ml, Synthomycin emulsion 2 ml and Dimexide 5% 4 ml is supplied to primary-site tumour through a perforated rubber tube. From 18th to 22th days, a mixture containing Mitomycin C 2.5 mg, Viferon ointment 2.5 mg, Vinilin 2 ml, Synthomycin emulsion 2 ml and Dimexide 5% 4 ml is supplied to primary-site tumour through a perforated rubber tube. After an 14-day recess, the therapeutical actions are repeated once again as specified.
EFFECT: involution of tumour, reduced radiation exposure and toxic by-effects of antitumour therapy.
SUBSTANCE: invention concerns ophthalmology and is intended for treatment of cornea edema and other bullous keratopathy manifestations. Medication includes leukocyte suspension of 1200-1500x103 cells/ml in autological serum, where mononuclears comprise 400-570x103 cells per 1 ml of suspension. Additionally medication includes cytokines, interleukines, b-FGF, TGF-b2 growth factors, endogenic TLR ligands, including heat shock proteins. Leukocyte suspension is obtained out of patient blood and activated by polyA:polyU TLR ligand complex (Poludan). Method of medication obtainment involves addition of polyA:polyU (Poludan) solution to patient blood sample at the ratio of 200-400 units per ml, cell activation at +18-+41 C° for 4-8 hours, centrifugation at 100-400G acceleration. Medication is injected in anterior eye chamber in amount of 0.5-1.0 ml until complete substitution of anterior chamber fluid by medication, by intracorneal method if required, and additionally activated autological cell suspension in autological serum is injected in amount of 1.0-2.0 ml paralimbally under conjunctiva in combination with antibiotic.
EFFECT: direct effect on damaged cornea endothelium, stopped or reduced cornea edema and other manifestations of early bullous keratopathy, increased visual acuity.
3 cl, 2 ex
SUBSTANCE: invention concerns medicine, particularly orthopedics and extracorporal treatment methods, and can be applied in aseptic whirlbone necrosis and Perther disease treatment. Method involves venous blood sampling in amount of 300 ml with further blood separation into erythrocyte mass and plasma. Obtained erythrocyte mass is diluted by 200 ml of 0.9% physiological solution and administered intravenously to patient. 100 ml or patient's plasma is placed in thermostat and incubated for 20 minutes at 37°C. Further 20 mg of vasoprostan medicine is added to incubated plasma, and obtained mix is administered to patient by drop infusion for 1.5-2 hours once a day for 10 days.
EFFECT: enhanced efficiency of the pathology treatment due to vasoprostan binding to plasma proteins and prolonged vasoprostan circulation in blood, resulting in arterial blood circulation improvement, venous stasis blocking in aseptic necrosis zone and significant acceleration of whirlbone acceleration.
2 tbl, 2 ex, 4 dwg
SUBSTANCE: invention concerns medicine, particularly vascular surgery, and can be applied in treatment of critical lower extremity ischemia. Method involves sampling of 400-500 ml of blood from vein. Blood is centrifuged for 15 minutes at 2000 rpm rate. Separated erythrocyte mass is dissolved by 200 ml of 0.9% physiological solution and returned to patient intravenously. 20 mcg of vasaprostan medication is added to 200 ml of plasma. Obtained mix is placed in thermostat and incubated for 20 minutes at 37°C, then injected to patient slowly by intravenous drop infusion for 2-3 hours once per day for 10 days.
EFFECT: elevated peripheral arterial pressure, ischemia grade regress, resulting in larger pain-free walking distance.
1 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely to oncology, and can be used in oesophageal carcinoma therapy. Substance of the invention consist that prior to radiation therapy, autoblood 400 ml is sampled. Plasma is separated by centrifugation. The first bottle is filled with autoplasma 40 ml and 5-fluorouracil 500 mg, while the second and third bottles are filled with autoplasma 40 ml, and the fourth with residual blood corpuscles and methotrexate 20 mg. The second and third bottles are frozen, and the first and fourth bottles are separately incubated for 40 minutes at 37°C. Then mixed autoblood and methotrexate from the fourth bottle are reinfused droplet-intravenously, mixed autoplasma and 5-fluorouracil from the first bottle are introduced into oesophagus through nasoesophageal probe and followed with the first session of radiation therapy. Intraesophageal autoplasma chemotherapy is carried out one day after the autoplasma bottle is unfrozen, added with 5-fluorouracil 500 mg and incubated in a thermostat. The entire therapy cycle is carried out for the following weeks of radiation therapy; irradiation is involved to TBD (total basic dose) 40-60 Gy at a single dose 2 Gy including 5 sessions a week.
EFFECT: method allows improving effectiveness of radiation therapy ensured by direct antineoplastic exposure of intraesophageal autoplasmochemotherapy and radiosensitisation of autohemochemotherapy.
SUBSTANCE: invention refers to medicine, namely to ceruloplasmin recovery, and can be used in making enzyme blood-plasma medicines. The disclosed method includes dissolution of feed stock in acetate buffer, enzyme sorption in an ion-exchange reactor with functional DEAE group, elution and following purification. Said ion-exchange reactor with functional DEAE group represents a sorbent on fixed styroldivinylbenzene matrix with co-graft DEAE group. And in purification, at first the dissolved enzyme is treated with a solvent-detergent mixture. Then the treated enzyme is immobilised on sulphopropylcation sorbent, washed in acetate buffer containing sodium chloride. The purified enzyme is eluted, while the feed stock is dissolved in acetate solution. The enzyme is sorbed in the ion-exchange reactor wherein as such sorbent on fixed styroldivinylbenzene matrix with co-graft DEAE group is applied. The enzyme is eluted and purified. In purification, the enzyme solution is treated with a solvent-detergent mixture, immobilised on sulphopropylcation sorbent, washed in acetate buffer containing sodium chloride. The purified enzyme is eluted.
EFFECT: method ensures virus safety of recovered medical product ceruloplasmin and allows improving end product yield.
9 cl, 2 ex
FIELD: food industry.
SUBSTANCE: invention relates to food industry namely to additives enriched with natural biologically active substances. Dietary supplement (DS) contains, in wt %: stabilised blood of reindeer, elk and Yakut horses - 55, antlers spirit extract (70% ethanol solution) - 10, flower honey solution (30%) - 35.
EFFECT: invention allows to obtain dietary supplement with increased biological activity, preventive, tonic and stimulating effect.