Method of producing flax polysaccharides

FIELD: chemistry.

SUBSTANCE: proposed method of producing flax polysaccharides involves extraction in distilled water under the effect of ultrasound at 30 kHz and 182276 W/cm intensity at 2732C. Ultrasonic treatment is done in 1217 minutes. The invention simplifies the extraction process, reduces expenses and cuts on time of obtaining polysaccharides.

EFFECT: significant increase in output and quality of polysaccharide.

2 cl, 4 dwg, 4 ex

 

The invention relates to chemical-pharmaceutical and medical industry, and in particular to a method for production of biologically active substances from medicinal plants.

A known method to obtain polysaccharides of flax seeds, including seed soaking water and seasoning mixture for days (Kochetkov NK Chemistry of carbohydrates / Nccadv - M.: Chemistry, 1967. - 672 S.).

The disadvantage of this process is its duration.

The closest is a method of producing polysaccharides flax, including extraction in distilled water, obtaining an extract of polysaccharides, subsequent drying and isolation of polysaccharides flax. The process of extraction of polysaccharides carried out for 2 hours at a temperature of 40C ground flax seed (Structural investigations of the neutral polysaccharide of Linum unitatissimum L. seeds mucilage / J. Warrand etc. // International Journal of Biological Macromolecules. - 2005. - No. 35. - P. 121-125).

The disadvantages of this method are the duration and the need for heating, which leads to degradation of polysaccharide chains.

The objective of the invention is to simplify the technological process of extraction, reducing costs, reducing time of receipt of polysaccharides.

The technical result of this method is the increase of the yield of the target product, as well as getting a better quality product.

Set for the ache and the specified technical result is achieved by the fact that in the method of producing polysaccharides flax, including extraction in distilled water, obtaining an extract of polysaccharides, subsequent drying and isolation of polysaccharides flax, according to the invention the extraction is carried out when exposed to ultrasound with a frequency of 30 kHz and an intensity of 182276 watts/cm2the extraction process is carried out at a temperature of 2732C. the duration of ultrasonic treatment is 1217 minutes. The selection of dry polysaccharides provide exposure to 70% ethanol, followed by filtration and drying under vacuum.

The use of ultrasound (US) with a frequency of 30 kHz can significantly reduce the time required to obtain polysaccharides of flax, as well as to increase the output data of valuable components. When using ULTRASOUND with a frequency below 30 kHz output polysaccharides flax is reduced, the use of ultrasound with a frequency above 30 kHz impractical due to excessive power consumption.

The use of ultrasound with intensity 182276 watts/cm2provides the minimum time to achieve a technical result, increased product yield. When using lower intensities of ultrasound exposure time is increased to achieve a technical result, and the increased intensity leads to degradation of secreted polysaccharides. The process when the temperature is re 27-32C allows to achieve the project objectives, namely cost reduction, and the technical result improved quality of the final product, because at this temperature does not occur inactivation of polysaccharides that allows you to get a quality product. The time of ultrasonic treatment is 1217 min is optimal for obtaining the maximum yield of pectin. Reducing the time to achieve the desired output, and the increase in the duration of ultrasonic treatment leads to the destruction of polysaccharide chains due to prolonged exposure to ultrasound.

The invention allows to avoid prolonged soaking (infusion) and additional heating plant materials and reduce the time to obtain polysaccharides of flax. The implementation of this method allows to reduce time of processing of raw materials, because in this process there is no need to use the pre-grinding.

The invention is illustrated by drawings, where figure 1 shows the dependence of viscosity solutions of polysaccharides flax seed intensity ultrasound; figure 2 - dependence of viscosity solutions of polysaccharides flax seed from the time of ULTRASONIC treatment, figure 3 - dependence of viscosity solutions of polysaccharides flax meal intensity ULTRASONIC, figure 4 - dependence of viscosity solutions of polysaccharides flax meal is from time ULTRASONIC processing.

The inventive method of extraction of polysaccharides flax was carried out as follows. Ultrasonic generator configured according to the intensity, the hanging plant materials were flooded with distilled water, the mixture was subjected to ultrasound. After ultrasonic extraction the plant material was separated by filtration. Then the polysaccharides was besieged three times the amount of 70%ethanol at room temperature. A dense precipitate was filtered, washed with 70%ethanol and then dried under vacuum. The obtained polysaccharide substances are white powder, which has been stored in a clean, tightly sealed container. As a source of polysaccharides used flax seeds and flax meal. To obtain a polysaccharide from the seeds require large values of the intensity and time of exposure on raw materials than when used as a source of polysaccharides flax meal.

Optimal conditions of the experiment were determined based on the values of viscosity and dry residue. Too powerful ULTRASONIC or too long exposure time destroy polysaccharides, as evidenced by the reduced viscosity of the obtained extract. The change of viscosity in each series of experiments has an extremum (Fig 1, 2). The comparison of these two relationships gives optimal conditions the implementation of the method. Based on these examples we can draw the following conclusions: the optimal conditions of ultrasonic influence are the intensity 274276 watts/cm2the processing time is 1217 minutes.

Example 1

Took a sample of flax seeds 5 g, were placed in a chemical beaker filled with distilled water 50 g (1:10). Ultrasonic treatment of the extracts was performed using an instrument IKASONIC U 50 control (30 kHz). The nozzle of the generator of ULTRASONIC waves was immersed in the chemical glass. Ultrasonic effects on flax seeds was performed with intensity 276 watts/cm2for 17 minutes at a temperature of 32C.

After processing is completed, the solution was filtered through a layer of gauze. Then there was the measurement of the viscosity of the obtained solution using a viscometer of Ballade. The viscosity is 0.4 PAC. To obtain dry polysaccharides obtained extract was besieged three times the amount (150 ml) 70%ethanol at room temperature of 25C. was Filtered through a double layer of gauze and dried under vacuum for 4 hours at a temperature not exceeding 60C. the Yield of polysaccharides flax 11,4%.

Example 2

The experiment was carried out analogously to example 1, except that the ultrasonic treatment was carried out with an intensity of 230 W/cm2within 16 minutes.

Polysaccharide yield of flax 7,1%.

Example 3

The experiment was carried out to analogichnimi 1 except the ultrasonic treatment was performed with the intensity of 320 W/cm2within 25 minutes.

Polysaccharide yield of flax 5,9%.

Therefore, the increased intensity leads to a reduction of the yield of polysaccharides flax.

Example 4

The experiment was carried out analogously to example 1 except that as the plant materials used flax meal, and ultrasonic treatment was performed with intensity 184 W/cm2within 14 minutes.

Polysaccharide yield of flax 6,1%.

When using as raw flax seed, it is advisable to carry out the extraction in the upper bounds of the claimed interval intensity ultrasound exposure and time. When using flax meal is advisable to carry out the extraction in lower boundaries of the intensity and time. This method will simplify the process of obtaining polysaccharides flax and reduce the cost of their production, to improve the yield and quality of the target product, as well as to reduce the process time and reduce the consumption of solvent.

This invention is in the stage of laboratory research.

1. The method of producing polysaccharides flax, including extraction in distilled water, obtaining an extract of polysaccharides, subsequent drying and isolation of polysaccharides flax, characterized in that the extraction is carried out when exposed to ultra the Vuka with a frequency of 30 kHz and an intensity of 182276 watts/cm 2the extraction process is carried out at a temperature of 2732C, the duration of ultrasonic treatment is 1217 minutes

2. The method according to claim 1, characterized in that the allocation of dry polysaccharides provide exposure to 70% ethanol, followed by filtration and drying under vacuum.



 

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SUBSTANCE: invention relates to a method for preparing pectin from vegetable raw. Method for preparing pectin involves milling and mixing sunflower heads and citrus husks, hydrolysis-extraction of mixture, separation of a liquid phase and isolation of the end product from its. Sunflower heads and citrus husks are extracted with dimethylformamide and liquid carbon dioxide before mixing separately and successively and corresponding extracts are separated. After separation of the second extract pressure is dropped abruptly up to atmosphere value, solid phases of sunflower heads and citrus husks are mixed and fed to hydrolysis-extraction. Invention provides enhancing quality of the end product due to elimination of undesirable taste and odor being without deterioration of its technological properties and without reducing the specific yield of pectin.

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EFFECT: improved preparing method.

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