Compositions lowering adipose body tissue, and foodstuff and beverage foods containing such compositions

FIELD: medicine.

SUBSTANCE: mannooligosaccharide composition is provided by mannan product hydrolysis. Mannooligosaccharide contains approximately 1 to approximately 10 monosaccharides with at least approximately 60 weight percents of monosaccharides is presented with mannose.

EFFECT: effectiveness to lower adipose body tissue and reduce body weight.

31 cl, 5 dwg, 7 ex

The present invention relates to compositions of mannooligosaccharides and foods and drinks containing mannooligosaccharide. In particular, mannooligosaccharide can be obtained by hydrolysis of the product (material) of mannan and included in the food and drinks. Mannooligosaccharide are effective to reduce the fatty tissue of the body and especially abdominal adipose tissue.

The LEVEL of TECHNOLOGY

In recent years westernized diet, lack of exercise and greater stress resulted in increased weight gain and obesity. Overweight and obesity are risk factors for diseases such as hyperglycemia, hypertension, and diabetes. Were developed supplements to limit weight gain and obesity. Many of these supplements are natural products or derived from natural products.

The coffee industry is one example of an industry that processes and produces a large number of natural products. Manufacturers of instant coffee and packaged coffee produce a large amount of used coffee residues. These residues mainly burn or destroy as industrial waste. Coffee beans and the resulting coffee residues contain water-insoluble β-mannan. M is noriginally (MOS) of the hydrolyzed the mannan of coffee are consumed by oligosaccharides. In the present invention using mannan to obtain compositions having the effects of reducing adipose tissue.

The INVENTION

Provide the composition of mannooligosaccharide, which is effective for obtaining the effect of reducing the fatty tissue of the body. The composition of mannooligosaccharide can be added to the compositions of foods, especially beverages, and be consumed orally. The introduction of mannooligosaccharides is effective to reduce total fat body tissue, assessment of percent body fat, at least 4%, preferably from about 4% to about 8%, abdominal adipose tissue by at least about 10%, preferably from about 10 to about 15%, and loss of body weight.

Provide the composition of mannooligosaccharides, which includes from about 1 to about 10 monosaccharides. At least about 60 weight percent of monosaccharides represents mannose, preferably at least 70 mass% of monosaccharides represents mannose and most preferably at least 80 weight percent of monosaccharides represents mannose. Mannooligosaccharide can also include one or more monosaccharides, such as glucose, galactose, fructose, and mixtures thereof. The composition of mannooligosaccharides can be included in foods, drinks, food, Leka the preparations and cosmetics. The composition of mannooligosaccharides provide in the form of a hydrolysate, which is formed by hydrolysis of the product of mannan. Product mannan derived from sources that include raw coffee beans, roasted coffee beans, used the remainder of the coffee, coconut flour, coconut flakes, coconut, palm Huacra, yams, Konica, lilies, daffodils, licorice, gum carob, guar gum and mixtures thereof. In an important aspect, the product source of mannan get from coffee materials and especially from used coffee residues.

The hydrolysate containing mannooligosaccharide, can be obtained using the methods of hydrolysis, such as acid hydrolysis, thermal hydrolysis, enzymatic hydrolysis, hydrolysis, microbial fermentation and combination of such methods. Thermal hydrolysis can be conducted at a temperature from about 200°to about 220°C., preferably about 210°C. the Hydrolysates can be bleached, deodorized and/or concentrated by contact with activated carbon, adsorption resin, ion exchange resins, ion exchange membranes and their combinations.

In another aspect provides a composition of foods, which includes mannooligosaccharide as described. In this aspect, the food composition includes at least about 1 gram of mannooligosaccharide the Yes on 100 grams of the composition of the food product. Mannooligosaccharide can be included in a variety of solid food and/or drinks. In an important aspect of mannooligosaccharide include coffee.

Also provide a way to obtain compositions of mannooligosaccharides. The method comprises the hydrolysis product of mannan in amounts effective to obtain a hydrolyzate containing mannooligosaccharide, where each mannooligosaccharide has from about 1 to about 10 monosaccharides with at least 60 mass% of the monosaccharide mannose, preferably about 70 weight percent of the monosaccharide mannose and most preferably about 80 weight percent of the monosaccharide mannose. Product mannan can be derived from raw coffee beans, roasted coffee beans, used coffee residues, coconut flour, coconut flakes, coconut, palm Huacra, yams, Konica, lilies, Narcissus, licorice, tar carob, guar gum and mixtures thereof. The preferred source of product mannan are used in the remainder of the coffee. The hydrolysate containing mannooligosaccharide, obtained using methods of hydrolysis, such as acid hydrolysis, thermal hydrolysis, enzymatic hydrolysis, hydrolysis, microbial fermentation, and mixtures thereof. The preferred method of hydrolysis includes thermal hydrolysis. The resulting hydrolysate may be the deodorized, discolored and/or concentrated using activated carbon, adsorbent resins, ion exchange resins, ion exchange membranes and their combinations. The resulting hydrolysate may be used in aqueous compositions, or may be dried or lyophilized.

In another important aspect provide a way to reduce the percentage of body fat and abdominal adipose tissue. The method includes the introduction of the composition of mannooligosaccharide in amounts effective to reduce the overall percentage of adipose tissue of the body, at least about 4%, preferably from about 4% to about 8% and the percentage of fat tissue in the abdominal area by at least about 10%, preferably from about 10% to about 15%. Mannooligosaccharide may be administered orally at a rate from about 1 to 10 grams per day, preferably from about 2 to 8 grams per day, and more preferably from about 3 to 6 grams per day as part of a food composition, such as, for example, a beverage, such as coffee.

BRIEF DESCRIPTION of DRAWINGS

Figure 1 illustrates the changes in the relative area total abdominal adipose tissue, based on the photos of the CT scan in the control group and the group MOS.

Figure 2 illustrates the changes in the relative area of subcutaneous abdominal adipose tissue in the photographs CT scanning control the Noah group and MOS.

Figure 3 illustrates the changes in the relative area of abdominal visceral adipose tissue in the control group and the group MOS.

Figure 4 shows the effect of MOS on pancreatic lipase.

Figure 5 illustrates the effect of MOS on the human level of triglycerides in plasma after oral intake of food with high fat content.

DETAILED DESCRIPTION

Provide compositions that give the effects of reduced adipose tissue and which may be regarded as having physiological functions in reducing obesity. Arrangements are oligosaccharides, which mainly include the mannose units. Such oligosaccharides on the basis of mannose or mannooligosaccharide are non-carcinogenic and low; they are selectively utilized by the intestinal microflora, reduce the level of serum lipid and provide absorption of minerals.

Definition

Provide mannooligosaccharide, which are oligosaccharides. As used in the present description, "oligosaccharides" refers to polymers of monosaccharides, which have a degree of polymerization (DP) of at least one, preferably, DP from about 1 to about 10, more preferably a DP of from about 2 to about 9 and more preferably a DP of from about 2 to about 6. Mannooligosaccharide with DP 1 is technically monoshiri the Ohm, but called oligosaccharide as a mixture of oligosaccharides can include multiple units of monosaccharides. Mannooligosaccharide will often include many of oligosaccharides with different degrees of polymerization.

Product mannan

As used in the present description, "mannan" refers to polysaccharides, which include monosaccharide mannose residues. Residues of mannose can be in the form of D-mannose, galactomannan, glucomannan and mixtures thereof. Polysaccharides, which include mannose, can be completely formed from the monosaccharide mannose residues or can be a combination of monosaccharide mannose residues and other monosaccharides, such as galactose, glucose and fructose.

Mannooligosaccharide can be obtained by hydrolysis of mannan. Sources of mannan include raw coffee beans, roasted coffee beans, used the remainder of the coffee, coconut flour, coconut flakes, coconut, palm Huacra, Yam, konjac, Lily, Narcissus, licorice, tar carob, guar gum and mixtures thereof. Coconut flour and coconut flakes can be obtained from coconut. Plant Palma Huacra South African Arecacease (Palmae), mannan yams and mannan Chinese yams - all provide the product source of mannan.

In an important aspect of roasted and ground coffee beans and used the remainder of the coffee, the cat is who left after brewing coffee, are the preferred source of mannan. Can be used with any type of coffee beans from any source. Examples of coffee, which can be used include Arabica, Robusta, Lively and the like. Can be used single type of coffee or a mixture of different types of coffee. Can also be used for coffee beans lower quality or size, including those with minimal or no General commercial values. In another important aspect mannan can be obtained from the residue of the extraction of the coffee obtained after extraction processing of roasted and ground coffee in the usual way to obtain a liquid coffee or instant coffee.

The hydrolysis product of mannan

The hydrolysis product of mannan may be carried out using methods of hydrolysis, which may include acid hydrolysis, thermal hydrolysis, enzymatic hydrolysis, hydrolysis, microbial fermentation, and mixtures thereof. Methods of hydrolysis using acid and/or high temperatures are described in the patent application of Japan No. Sho 61-96947 and Hei 02-200147, which is incorporated into this description by reference. In addition, the acid hydrolysis methods described in U.S. patent No. 4508745 and high temperature hydrolysis described in EP 0363529, both of which are included in the present description by reference.

Used remain the key coffee can be hydrolyzed in the reaction vessel with or without acid and with or without pressure. The hydrolysis can also be carried out in any reaction vessel for this treatment, such as, for example, a reactor with a pulsating flow. Hydrolysis effective for hydrolysis of mannan, which may have DP 10-40 or higher to oligosaccharides with DP 1-10.

The enzymatic hydrolysis may be carried out by suspendirovanie product of mannan in the aquatic environment and the addition of the corresponding commercially available enzymes, such as cellulase and hemicellulase. The enzymatic hydrolysis may be carried out using standard conditions well known to the ordinary expert in the field of technology.

Microbial fermentation can also be used for the hydrolysis product of mannan. The product of mannan can be fermented by microorganisms that produce enzymes that can hydrolyze mannan. Can be used microorganisms that produce enzymes, such as cellulase and hemicellulase. One example of a suitable microorganism is Basidomycota.

The resulting hydrolyzate product of mannan can be used as is or can be further purified activated carbon, adsorption resin, ion exchange resins, ion exchange membranes and their combinations. Bleaching and/or deodorizing hydrolysates can be carried out by contact hydrolysates activated carbon or any other material resin absorbing type. Desalting and neutralization can be carried out using ion-exchange resins and/or ion-exchange membranes. Can also be used combinations of such methods. Further purification can be carried out at higher dose level or when the hydrolysate should be used in a certain type of food or drinks.

Clinical trials

In trials with drugs MOS, body weight and waist circumference in groups of MOS tended to decrease after 12 weeks compared with the start time. The average weight loss ranged from about 0.5 to about 1.0 kg and waist circumference decreased by from about 1.5 to about 3,0 see the Percentage of adipose tissue body DEXA decreased from about 4% to about 8% in the MOS groups after 12 weeks compared with the start time and significantly different from the placebo group at the end of the observation period. Area total abdominal adipose tissue and the area of visceral adipose tissue on CT scan was significantly decreased in the MOS groups compared with the placebo group after 12 weeks with a decrease in percent body fat from about 10% to about 15% from the beginning of the study. Characteristic evaluation CT scan of adipose tissue on a relative basis, in addition, demonstrated significant changes in the abdominal area. The results showed that coffee n pitoc, containing MOS, reduced the percentage of adipose tissue of the body and abdominal adipose tissue in humans, which over time can be reflected in the reduction of body weight.

If there is no intention to be bound by any theory, the reduction of abdominal adipose tissue may be due to one or more of the following reasons: (1) propionic acid, which is enteric fermentation MOS, can inhibit fatty acid synthesis in the liver, (2) MOS can inhibit the absorption of lipids in the small intestine, causing an accumulation of fat tissue in the body, for metabolizirovannom and (3) may increase the excretion of fat tissue by volume of the stool, thus reducing abdominal fat tissue.

EXAMPLES

EXAMPLE 1:Clinical studies MOS - Investigation 1

1. The drug MOS

Used the remainder of the coffee hydrolyzed in the reaction vessel at a temperature of 220°C. the Hydrolyzed product was decolorized powder activated carbon (Umehachi; Taihei Chemicals, Osaka) and absoluely cation exchange resin (SKIB; Mitsubishi Chemicals, Tokyo) and anion-exchange resin (WA30; Mitsubishi Chemicals). Monosaccharides were removed using chromatography with activated charcoal with step gradient of water and 10.0% (V/V) ethanol. The purified solution was concentrated in a rotary evaporator and liofilizirovanny. A mixture of OS (degree of polymerization (dp) 2:21,2%, dp3:22,2%, dp4: 21,2%, dp5:16.2% and dp6 or more: 19,2%) used for clinical trials.

2. The test drinks

Composition of coffee drinks was following.

Coffee drink Packed in 900 ml bottles from PET (polyethylenterephtalate). Test the drink contained 1 g MOS in 100 ml. Each patient received 300 ml of the tested coffee drink (containing 3.0 g MOS) per day. Solid corn syrup was added to control coffee drink as a placebo instead of MOS. The tested coffee drinks were received with the same level of substances of coffee, so that between them there were differences in the aroma. Both drinks were also awarded for having uravnoveshivanie level of caffeine, because you know that caffeine increases energy consumption. The level of caffeine amounted to 45.4 mg/100 ml in the control of coffee and 46.8 mg/100 ml in the tested coffee, respectively.

3. Patients and schedule studies

The present study has a design placebo-controlled, double-blind study. Volunteers were subjected to a medical and physical examination. Received a fasting blood sample for analysis of serum lipid.

Were selected thirty volunteers (male 15, female 15). They belonged to the category of obese persons 1 (25 kg/m²< BMI < 30 kg/m²in accordance with the classification of obesity of the Japanese Society for the Study of Obesity and the category with hypercholesterolemia (total cholesterol serum > 180 mg/100 ml). They were divided into 2 groups according to BMI and the level of total serum cholesterol physician not associated with this study.

After a one-week follow-up period patients were given coffee beverage for 12 weeks. They visited the clinic on the first day, after 4, 8 and 12 weeks. On each visit to the clinic, they were subjected to a medical and physical examination. Collected samples of blood and urine glucose and estimated the area of abdominal adipose tissue using computed tomography scan (CT-scan).

Patients measured on 300 ml of coffee on IDA (MOS or placebo) measuring Cup and drank it every day without adding milk or cream. The time consumption is not limited. Patients were instructed that during the study they do not have any way to change your usual diet. In addition, they were restricted from consumption of foods and drugs that affect blood lipids. At every visit to the clinic they were required to do not eat or drink anything except water from 9.00 PM to complete the survey.

4. Write diaries power

Patients were given the scale weight, disposable camera and pedometer. Patients were asked to record all meals, including snacks between meals, the weight of each dish, to photograph every meal and count each day, the number of completed steps in the 3 days before the clinic visit. Nutrient intake was calculated as the records of meals and photos using the Microsoft Excel Add-in “Excel Eiyou-kun ver. 3.0” (Kenpakusya; Tokyo, Japan). Volunteers also recorded daily consumption of alcohol.

5. Physical examination

Conducted physical examination with recording of height, weight, hip circumference and waist and thickness of the subcutaneous adipose tissue. Hip circumference and waist was measured at the exhalation and standing in accordance with the method of the Japanese Society for the Study of Obesity (Tokunaga et al., Int.J. Obesity, 7, 437-445(1983)). The thickness of the subcutaneous adipose tissue were measured on the square is che and under the scapula using a compass. The Body mass index (BMI) was calculated in accordance with the following equation: BMI (kg/m²) = [body weight (kg)]/[height (m]².

6. The study area of abdominal adipose tissue

The definition of the area of abdominal adipose tissue by CT-scan was performed using a method Tokunaga (Int.J. Obesity, 7, 437-445 (1983)). All CT studies were performed using CT-W450 (Hitachi Medico Inc., Japan). X-ray pictures made with the use of tube voltage 120 kV, tube current of 90 mA; window level 0; and the width of the window 1000. The total area of the adipose tissue area of the adipose tissue and the area of visceral adipose tissue was determined by CT images using assessment tools visceral adipose tissue software "fat Scan ver.2" (N2 System Inc., Japan).

7. Blood test

Blood test in this study consisted of leukocytes, erythrocytes, hemoglobin, MCV, MCH, MCHC and platelet counts. Total protein, the ratio A/G, albumin, total bilirubin, AST (GOT), Alt (GPT), alkaline phosphatase, LDH, γ-GT, total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, uric acid, urea nitrogen, creatinine, Na, K, Cl, Ca, IP, Mg, fasting blood sugar, total lipids, free fatty acids, peroxides, lipids, HbA1c, and insulin were measured using biochemical blood tests.

8. Statistical analysis

The result of the analysis are presented as mean values with standard errors. Comparison of mean values between each of the groups were analyzed using paired t-test, when the differences of the data were consistent with a normal distribution and were analyzed using criteria prescribed ranks Wilcoxon, when the differences of the data did not conform to a normal distribution. Fluctuations in the area of adipose tissue during the study period are presented as relative values compared with the data at 0 week. Differences in mean values between two groups were subjected to variance analysis and then when calculating using or t student test or t-test, Welch. The values of p < 0,05 were assessed as valid.

9. Nutrition during the study period

Statistical analysis was performed in 29 patients who were divided into two groups: one group of 15 patients who were given the control drink (hereinafter called "control group"); and another group of 14 patients who were given a drink containing MOS (hereinafter the "study group"). One patient was excluded from the research group because of its extremely irregular diet during the study period. Write the introduction showed that the degree of consumption amounted to 99.9% for the control group and 99.7% for group studies, demonstrating the absence to the reliable differences between the two groups. Has not been a significant consumption of alcohol or drugs during the study period. The results of the study of nutrition revealed that there was a small significant difference in the diet during the study period for both groups. The average energy consumption per day ± standard error amounted 1997±77 kcal/day for the control group and 2066±72 kcal/day for group study. The level of physical activity, which is shown by pedometers, showed no significant changes in both groups during the study period.

10. Side effects

In the physical condition of the patients was not observed side effects associated with the introduction of the test drink at any time during the study period. Medical consultations did not reveal the subjective/objective symptoms or abnormal values due to the introduction of drinks during the study period.

11. Physical assessment

Was not confirmed significant differences between the control group and the study group. Changes in physical parameters during the study period are shown below.

Did not observe statistically significant differences in weight, BMI or subcutaneous adipose tissue in the middle part of the extensor side of the shoulder for each group. The reduction of waist circumference for both groups was statistically significant when compared with before applying the drinks, however, were not statistically significant differences between the two groups. The thickness of the subcutaneous adipose tissue around the rear under the shoulder blade for the treatment group was significantly decreased (p < 0,05) at the 12th week compared to the beginning of the application, but there were no statistically significant differences between the two groups. The differences between the two groups were confirmed as minimal and unreliable.

12. The area of abdominal adipose tissue on CT scan

One patient from the control group and one patient from the study group were excluded from analysis of transverse sections of lipids, as the perfect image of the CT scan could not be obtained due to the large size of the waist of the patient. Statistical analysis was performed in 27 patients, 14 of the control group and 13 from the studied group. Changes in the area of abdominal adipose tissue during the study period are shown in Table 4.

We detected no significant differences between groups with respect to the total area of adipose tissue, the area of the subcutaneous adipose tissue and area of visceral adipose tissue at the beginning of the introduction (0 week). During the period of the study, analysis of the area of adipose tissue area of the adipose tissue of the control group was significantly decreased on the 8th week (p < 0.05) as compared with the beginning of the introduction. In the study group, a significant decrease (p < 0,01) was found after 8 and 12 weeks in relation to the total area of adipose tissue, the area of the subcutaneous adipose tissue and area of visceral adipose tissue. The decrease from prior consumption at 0 week, in the MOS group was greater than in the control group. In the study group were clearly shown to significantly lower values for the total area of adipose tissue on the 8th week (p < 0.05) and week 12 (p < 0.01) compared with the control group (Figure 1). In the study group also shows a significant decline in the area of subcutaneous adipose tissue and area of visceral adipose tissue at 12 weeks the Le (p < 0.05 for both) compared with the control group (Figure 2 and 3).

13. Research results blood

Two patients from group studies were excluded from the analysis of blood, as they ate Breakfast and drank soft drinks after 9.00 PM on the 12th visit to the clinic. Change the values of blood examinations during the study period are shown in table 5, and change the values of biochemical blood tests are listed in table 6.

At the beginning of the introduction (0 week) found no significant differences between the two groups on any of the items in the study of blood and biochemical analysis of blood. For the studied parameters associated with the lipids of human serum, the results of blood tests and biochemical studies of blood group studies have shown a significant reduction of LDL cholesterol from the beginning of the introduction to 12 weeks (p < 0,05). Although we detected no significant difference is between the two groups, in the study group there was a trend towards lower values for concentrations of total cholesterol (p < 0,054) and LDL cholesterol (p < 0,062) at week 12 compared with the control group. Although compared to the beginning of the introduction there were several statistically significant differences in performance, other than associated with serum lipid person, such differences were in the standard range.

EXAMPLE 2:The effect of liquid coffee containing mannooligosaccharide

Further analyzed the data of tested patients in Example 1, which had a BMI > 26,4 (2 men and 5 women). After the consumption of liquid coffee containing MOS, the weight ratio of the fatty tissue of the body and BMI decreased significantly, as shown in the table. Therefore, liquid coffee, containing MOS (3 g/300 ml), showed the presence of effects of reduced adipose tissue of the body and effect against obesity in humans.

EXAMPLE 3Clinical studies MOS - Study 2

This study, like the study described in Example 1, except that used two doses MOS (MOS 3.0, or 6.0 grams/day). All estimates are the same as described in Example 1 with the addition of dual energy x-ray absorptiometry (DEXA).

The study has a design placebo-controlled, double-blind study. Volunteers were subjected to a medical and physical examination. Received a fasting blood sample for analysis of serum lipid before the study.

As patients to ASCS is adowanie were selected seventy-two volunteers (36 men; 36 women). Volunteers were described as having obesity group 1 (25 kg/m²< BMI < 30 kg/m²in accordance with the classification of obesity of the Japanese Society for the Study of Obesity. They were divided into three groups according to BMI and the ratio of adipose tissue of the body by a physician not associated with this study.

After one week follow-up period patients were given coffee beverage for 12 weeks. Patients measured on 300 ml coffee drink (MOS or placebo) using a measuring Cup and drank it every day without adding milk or cream. The drink was received together with the meal. Patients were instructed that during the research, they should not change in any way to their usual diet. In addition, limited in their consumption of products and medicines that can affect blood lipids.

Patients visited the clinic at day 0 and then at 4, 8 and 12 weeks. On each visit to the clinic was required of them do not eat or drink anything except water after 9 PM to complete the survey. At each visit patients were subjected to a medical and physical examination. Percentage of total fatty tissues of the body were measured by dual energy x-ray absorptiometry (DEXA). In addition, at day 0 and after 12 weeks received image is s blood and urine glucose and measured the area of abdominal adipose tissue using computer tomographic studies (CT scan).

The results of this Study 2 partially similar to the results of Study 1 (Example 1). The decrease in body weight, waist circumference, ratio of adipose tissue of the body, abdominal adipose tissue and visceral adipose tissue was observed in all the MOS group. In addition, total adipose tissue of the body, as determined by DEXA, was reduced on average by 6% in the MOS group after 12 weeks, when compared with the beginning of the study and was significantly different from the control group. To date there has been reactions associated with the dosage on the basis of experimental work.

EXAMPLE 4Effect of MOS on the activity of pancreatic lipase

Composition MOS were obtained as described in Example 1. The distribution of DP songs of mannooligosaccharides (MOS)used for this example was DP1: 2,4%; DP2: 26,6%; DP3: 20.2%; and DP4: 17,8%; DP5: 10,9%; DP6: 8,9%; DP7: 6,0%; DP 8: 3,6%; DP9: 1.9% and DP10: 1.7 percent. The content of mannose residues in the carbohydrate chain was 90%.

Measurement of the activity of pancreatic lipase.

The lipase activity was determined by measuring the ratio of the release of oleic acid from triolein. Briefly, a suspension of triolein (80 mg), phosphatidylcholine (10 mg) and human beings need it to acid (5 mg) in 9 ml of buffer 0,1M N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES) (pH 7.0)containing 0.1 m NaCl, and treated with ultrasound for 5 minutes. Such processed what Ultrazvuk suspension of the substance (100 μl) were incubated with 50 μl (10 units) pancreatic lipase and 100 µl of different concentrations of MOS solution for 30 minutes at 37°C in a final volume of 250 μl. The number of released oleic acid was determined in the normal way. Inkubiruemykh the mixture was added to 3 ml aliquot of the mixture 1:1 (V/V) of chloroform and n-heptane containing 2% (V/V) methanol, and was extracted by shaking the tubes horizontally for 10 minutes and the upper aqueous phase was removed by suction. Then add the copper reagent (1 ml) to the lower organic phase. The tube was shaken for 10 minutes, the mixture was centrifuged at 2000 g for 10 minutes and 0.5 ml of the upper organic phase, which contains a salt of copper extracted free fatty acids, was treated with 0.5 ml of 0.1% (wt./about.) bathocuproine in chloroform containing 0.05% (wt./about.) 3(2)-t-butyl-4-hydroxyanisole. Then measured the absorption at 480 nm. The lipase activity was expressed per ml of reaction mixture per hour. As shown in figure 4, MOS inhibited the activity of pancreatic lipase dose-dependent at concentrations of 1-5 mg/ml; 5 mg/ml inhibited by about 70%. These in vitro findings suggest that MOS is an effective agent that inhibits the absorption of lipids.

EXAMPLE 5: Triglycerides plasma after the consumption of food with high content of fat in studies in humans

Ten healthy men volunteers (all men) were divided into 2 groups and carried out a double-blind crossover design. Each group was injected MOS or placebo in p is pout series. A week later, a second series (placebo or MOS) was administered Vice versa. They did not eat during the night and is administered orally 200 g of food with high fat content, consisting of corn soup containing oil and fat (fat 40 g) with or without MOS (3 g). The blood received from the veins before and after (1H, 2H, 3h, 4h, 5h and 6h) oral administration and was subjected to measurement of triglycerides. As shown in figure 5, the increase in plasma triglycerides was significantly decreased after 3 hours after injection. Consumption of MOS in the diet with high fat content was effective in reducing postprandial increase in plasma triglycerides. These results suggest that consumption of MOS is effective to reduce the consumption of fat in the human body.

EXAMPLE 6The effect of mannooligosaccharides on the ratio of adipose tissue in the body

The effect of MOS on the ratio of adipose tissue in humans studied using healthy Japanese people (6 men and 7 women). One group consisted of 6 patients treated MOS at 1.0 g/day. The second group consisted of seven patients treated MOS 3.0 g/day. Evaluation was carried out on the basis of changes in percent body fat of the body. The percentage of fat body tissue was analyzed using the methodology of electrical resistance. The table below shows the results of the effect of consumption of MOS on p is ocent adipose tissue of the body.

After receiving MOS percent of fat body tissue was decreased, as shown in the table below. MOS (1.0 g/day and 3.0 g/day), thus, showed the effect of reducing the fatty tissue of the body in humans.

Changes in the ratio of fat body tissue (%)

EXAMPLE 7The effect of liquid coffee containing mannooligosaccharide

The effect of liquid coffee containing mannooligosaccharides on the individual's body weight and percent body fat were studied using ten healthy Japanese using the same coffee samples containing MOS, as described in Example 6. Patients received liquid coffee containing MOS, and drank 900 ml liquid coffee each is the tier for 4 weeks (i.e. 3 bottles/day to a total of 9 g MOS/day). Conducted evaluations on the basis of changes in body weight, percentage of body adipose tissue and BMI, as in Example 6. The table below shows the results of the effect of consumption of liquid coffee containing MOS, on body weight, percentage of body adipose tissue and BMI.

After the consumption of liquid coffee containing MOS, body weight, percent body fat body and BMI decreased significantly, as shown in the Table. Liquid coffee containing MOS, therefore, showed an effect of reducing the fatty tissue of the body and effect against obesity in humans.

The effects of liquid coffee containing MOS on body weight, the ratio of adipose tissue and BMI

1. The composition of mannooligosaccharide, effective to reduce the fatty tissue of the body, including the hydrolyzate obtained by hydrolysis of the product of mannan, the composition of mannooligosaccharide has from about 1 to about 10 monosaccharides, where at least about 60 wt.% the monosaccharide is a mannose, when this composition is effective to reduce the ratio of adipose tissue of the body by at least about 4% and abdominal adipose tissue by at least about 10% in humans when consumed by man in the amount of at least from about 1 to about 10 g/day.

2. The composition of mannooligosaccharide according to claim 1, where the product of mannan provided from a source selected from the group consisting of raw coffee beans, roasted coffee beans, used coffee residues, coconut flour, coconut flakes, coconut, palm Huacra, yams, Konica, lilies, Narcissus, licorice, tar carob, guar gum and mixtures thereof.

3. The composition of mannooligosaccharide according to claim 2, where the product of mannan derived from used coffee residues.

4. The composition of mannooligosaccharide according to claim 1, where the hydrolysate containing mannooligosaccharide, obtained using the method of hydrolysis, Ibraimova from the group consisting of acid hydrolysis, thermal hydrolysis, enzymatic hydrolysis, hydrolysis, microbial fermentation and their combinations.

5. The composition of mannooligosaccharide according to claim 1, where the hydrolysate is purified with activated carbon, adsorption resins, ion exchange resins, ion exchange membranes, or combinations thereof.

6. The composition of mannooligosaccharide according to claim 1, where at least 70 wt.% composition of mannooligosaccharide represents mannose.

7. The composition of mannooligosaccharide according to claim 1, where at least 80 wt.% composition of mannooligosaccharide represents mannose.

8. The composition of mannooligosaccharide according to claim 1, where the composition of mannooligosaccharide included in foodstuff, drinks, medicines, feed, cosmetics, and mixtures thereof.

9. The composition of mannooligosaccharide according to claim 1, additionally comprising at least one monosaccharide selected from the group consisting of glucose, galactose, fructose, and mixtures thereof.

10. The composition of food products, including mannooligosaccharide, and mannooligosaccharide includes from about 1 to about 10 monosaccharides, where at least about 60 wt.% monosaccharides represents mannose.

11. The composition of claim 10, where the composition of the food product includes at least about 1 g mannooligosaccharide on 100 g of the composition of food.

13. The composition according to item 12, where the drink is coffee.

14. The composition of claim 10, where mannooligosaccharide is a hydrolyzate obtained by hydrolysis of the product of mannan.

15. The composition according to 14, where the product of mannan derived from a source selected from the group consisting of raw coffee beans, roasted coffee beans, used coffee residues, coconut flour, coconut flakes, coconut, palm Huacra, yams, Konica, lilies, Narcissus, resin carob, guar gum and mixtures thereof.

16. The composition according to item 15, where the product of mannan derived from used coffee residues.

17. The composition according to 14, where the hydrolysate containing mannooligosaccharide, obtained using the method of hydrolysis selected from the group consisting of acid hydrolysis, thermal hydrolysis, enzymatic hydrolysis, hydrolysis, microbial fermentation and their combinations.

18. The composition according to 14, where the hydrolysate is purified with activated carbon, adsorption resins, ion exchange resins, ion exchange membranes, or combinations thereof.

19. The composition of claim 10, where at least 70 wt.% mannooligosaccharide represents mannose.

20. The composition of claim 10, where at least 80 wt.% mannooligosaccharide is Soboh is mannose.

21. The way to reduce the ratio of adipose tissue body or abdominal adipose tissue in humans, including the introduction of the composition of mannooligosaccharide man, and the composition of mannooligosaccharide includes from about 1 to about 10 monosaccharides, where at least 60 wt.% monosaccharides represents mannose, while the composition of mannooligosaccharide effective to reduce the ratio of adipose tissue of the body, at least 4% and abdominal adipose tissue, at least about 10%.

22. The method according to item 21, where mannooligosaccharide administered orally in an amount of from about 1 to 10 grams per day.

23. The method according to item 22, where mannooligosaccharide be administered orally in the composition of food.

24. The method according to item 23, where the composition of the food product is a beverage.

25. The method according to item 21, where mannooligosaccharides is a hydrolyzate obtained by hydrolysis of the product of mannan.

26. The method according A.25, where the product of mannan derived from a source selected from the group consisting of raw coffee beans, roasted coffee beans, used coffee residues, coconut flour, coconut flakes, coconut, palm Huacra, yams, Konica, lilies, Narcissus, licorice, tar carob, guar gum and mixtures thereof.

27. The method according to p, where the material of mannan derived from used coffee residues.

28. The method according A.25 where to get the hydrolysate, containing mannooligosaccharide using the method of hydrolysis selected from the group consisting of acid hydrolysis, thermal hydrolysis, enzymatic hydrolysis, hydrolysis, microbial fermentation, and combinations thereof.

29. The method according A.25 where hydrolyzed purified using activated carbon, adsorbent resins, ion exchange resins, ion exchange membranes or combinations thereof.

30. The method according to item 21, where at least 70 wt.% mannooligosaccharide represents mannose.

31. The method according to item 21, where at least 80 wt.% mannooligosaccharide represents mannose.