Method of defining intraperitoneal pressure in experiment with small laboratory animals

FIELD: medicine.

SUBSTANCE: fine rubber balloon with sealed tube fixated in the lumen is positioned in peritoneal cavity. Loose tube end is lead out through subcutaneous tunnel to rear neck surface, laparotomic wound is oversewn. Dynamic registration of intraperitoneal pressure is performed by connecting loose tube end to gauge. Gauge includes vertical stand with gauge scale of water pressure gauge, to which vein catheter and tube system is connected vertically.

EFFECT: defining intraperitoneal pressure of small laboratory animals in different positions, separate definition of inlet and outlet pressure.

2 dwg, 1 ex

 

The invention relates to medicine, namely to experimental medicine, and can be used to determine the intra-abdominal pressure (IAP) in the experiment using small laboratory animals (rats, Guinea pigs).

Intra-abdominal pressure is an important indicator reflecting the status of organs of the abdominal cavity during deployment acute peritonitis and other pathological processes. Dynamic monitoring of the IAP in the experiment allows us to estimate the degree of disorders of intestinal motility, stagnation content and to judge about the oppression of microcirculation in intestinal wall.

The prototype of the invention is the method of determining the IAP in the experiment in dogs (AIC S.I. Ways to improve the results of surgical treatment of diffuse purulent peritonitis (Clinical-experimental study). Abstract. Diss. ... Dr. med. Sciences. - Omsk, 1992). The method involves the location of the rubber balloon into the abdominal cavity of the animal. Pre canister filled with liquid and of its lumen output after laparotomic wound up. The measurement is carried out in the horizontal position of the animal in millimeters water column by means of fixing to the tube gauge. However, the method is unsuitable for use in experiments on small laboratory animals (rats, Guinea pigs), as the rat is there to pull the tube from the abdominal cavity, and the device is unable to detect small fluctuations in the IAP. Thus, the application of the method Siejka for small laboratory animals is technically complex and uninformative.

The technical result - the definition of intra-abdominal pressure in experiments on small laboratory animals in various provisions of the animal, separately on the inhale and exhale.

The proposed method is illustrated by the following drawings: figure 1 shows a recording device, a General view, figure 2 - measuring device, General view.

The proposed method of measuring IAP is as follows. As a recording device using a spray containing 1 finger from the standard examination medical gloves and the tube 2 from the system for intravenous infusion a length of 10-12 cm To the end of the tube 2, is placed within the lumen of the balloon, made more lateral notches, and at a distance of 2 cm from the end in the lumen of the tube longitudinally placed plastic cannula 3 of the disposable syringe. The mouth of the balloon is fixed on a nylon ligature 4 on the tube in the place where you installed the cannula 3. When this cannula plays the role of a rigid frame and prevents compression of the tube when tightening the ligature. Both ends of the ligature leave a free length of at least 5-6 cm after delaying for a next item is Diwaniya them to the abdominal wall of the animal (figure 1). In the manufacture of the device under aseptic conditions using sterile materials and tools.

As a measuring device using vertical tripod 5 with a measuring range from water manometer, which in a vertical position attached venous catheter 6 with a diameter of 1 mm, the Bottom end of the catheter through the adapter 7 is connected with a rubber tube 8 from the system for intravenous infusion length 15 see the Opposite end of the tube is connected to the tee 9, the remaining ends of which are attached two rubber tubes 10-11 length of 15 cm To the tube 10 is attached to the syringe 12 with a capacity of 20 ml, which is used for filling the system with fluid and remove air out of it. The tube 11 is attached to the adapter 13 for connection to a recording device - end of the tube, extracted on the neck (figure 2). For use adapters from the system for intravenous infusion.

Before you begin recording device is calibrated by determining the maximum amount of liquid placed in the container without increasing the pressure therein. For this purpose the free end of the tube connected to the adapter 13. Through syringe 12 the system is filled with colored liquid (furatsilina). The spray feature is free on the zero mark of the measuring line. The liquid level align the zero mark, then the tubes 2 and 11 impose clips, tube 2 is disconnected from the adapter 13. The liquid from the container and tube are produced to measure the amount of data recorded in the study Protocol. This is the end of the preparation stage of the device.

At the second stage in aseptic conditions under ether inhalation anesthesia perform verhnesadovoe laparotomy and peritoneal cavity of rats freely placed the canister. From the edge of the laparotomic wound on the anterolateral surface of the chest with the transition to the rear surface of the neck of the animal by blunt form the tunnel in the subcutaneous tissue. The free end of the tube 2 is carried out through the tunnel and display on the rear surface of the neck at a distance of 1.5-2 cm from the skin. The remaining free ligatures 4 at the neck of the balloon is stitched to the abdominal wall of the animal. Laparotomic wound and incision on the neck tightly sutured anchor nylon sutures. Through the open end of the tube 2 in the spray injected fluid in an amount which was determined during calibration. The end of the tube is closed by a stopper. The location of the tube in the neck do not allow the animal freely in the cage, to reach him with their teeth.

When measuring pressure of the animal is placed on a level with the zero mark of the measuring scale of the device. The tube 2 impose the clamp, the tube is removed and connect the hose to the adapter 13. The clamps with the tubes removed. The movement of the column of colored fluid in the catheter along the measuring line shows the change in intra-abdominal pressure in millimeters of water column. Data capture in the study Protocol. The high sensitivity of the measuring system allows to determine the pressure separately on the inhale and the exhale, including various provisions of the animal. The system design allows to connect the animal to the measuring system and to observe the dynamics of intra-abdominal pressure for a long time. However, you must make a correction on the developing adhesions around the canister. The correction factor is calculated using the control animals.

The proposed method is simple and reliable in operation. In the intervals between measurements of intra-abdominal pressure, the animal is placed in a cage in natural conditions. The location of the free end of the tube on the back of the neck does not allow the animal to hurt himself. The use of thin venous catheter allows you to record even the slightest fluctuations in intra-abdominal pressure. To develop and test method conducted experimental studies on 57 white rats male Wistar weighing 180-250 g, 12 Guinea pigs weighing 350-400 g

Example.

The white rat with the Mets weight 220 g taken in the experiment for the determination of the credit in the dynamics of the unfolding of peritonitis. In aseptic conditions under inhalation of ether anesthesia in abdominal cavity is placed a recording device with a rubber bulb with a capacity of 9 ml, the tube is brought out through a subcutaneous tunnel on the rear surface of the neck. Wounds sutured interrupted sutures. After the operation and exit of the animal from anesthesia indicators of credit were as follows: the position of the animal standing - 55 mm vods on the inhale and 50 mm vods on the exhale, lying respectively 65 and 60 mm Vogt Modeling of peritonitis was once intraabdominal administration 20% autoclaves suspended in 2 ml. during deployment of the clinical picture of peritonitis was performed measuring IAP in the dynamics. Within 1 hour after the operation indicators IAP was: standing - 70 and 60 mm Vogt (inspiratory and expiratory), in the supine position - 75 and 70 mm Vogt further during 6 hours of observation has been an increase in IAP standing up to 85 and 75 mm vods To the end of the first day of increased IAP to level 100 and 90 mm Vogt (standing) and 110 and 95 mm Vogt (in lying position). By the end of the third day of observation indicators credit standing was 140 and 120 mm Vogt (inhaling and exhaling), and in the lying - 160 and 145 mm Vogt animal Subsequently died with symptoms of progressive multiple organ failure, when patoma the essay examination revealed characteristic manifestations of widespread peritonitis and endogenous toxicosis. Thus, during deployment of the pathological process in the abdominal cavity, when using the proposed method was observed to be significantly (p<0.001) and a twofold increase in intra-abdominal pressure relative to the basal level.

The method of determining the intra-abdominal pressure in the experiment, including the location in the abdominal cavity of the animal recording device in the form of a spray of thin rubber with a tightly fixed in it the tube, characterized in that on the end of the tube placed in the lumen of the balloon, made of lateral notches and into the lumen at a distance of 2 cm from the end of the longitudinally placed a plastic cannula, the balloon is fixed a ligature to the tube at the location of the cannula, after which the free end of the tube is brought out through the subcutaneous tunnel to the rear surface of the neck, laparotomic wound tightly sutured, and for measuring pressure using a device comprising a vertical tripod with measuring range from water manometer, which in a vertical position attached venous catheter, the lower end of which is connected with a rubber tube, the opposite end of which is connected to the tee, to the remaining ends of which are attached two rubber tubes, one of which is connected with a syringe, and the other through the adapter connected to the end of the tube, placed on the neck of the LM is now.



 

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