Method of obtaining medication, possessing antioxidant, cholagogic, hepato radio- and nephroprotective, antiradical, wound-healing activity
SUBSTANCE: wastes of confectionery industry production, milled husk of cacao beans, are drawn in water at temperature 20°C for 2 hours with following extraction by hot water (80-90°C) in battery of four extractors, thickening of extract and drying are carried out.
EFFECT: extension of medication activity spectrum and decrease of power consumption.
The invention relates to the pharmaceutical industry, namely the production of therapeutic and prophylactic drugs from edible raw materials.
The technical result of the invention is to develop a method of obtaining funds simultaneously possessing antioxidative, choleretic, hepato-, radio - and nephroprotective, antiradical, wound-healing effect.
A method of obtaining from the husk of the cocoa beans, cocoa-Vell means having hepatoprotective, choleretic, antioxidant and radioprotective action, which is as follows: 50 kg cocoa-Vell is extracted with 250 kg of hot water (80-90°C), water extraction will shuchat, dried, crushed and receive 11,2 kg light brown amorphous powder with a yield of 22.4% .
A significant disadvantage of the known method are significant energy costs at the stage of condensation of the extract, since the total amount warded off the water is up to 750 kg per 33,6 kg of the desired product.
The aim of the invention is the extension of the spectrum of pharmacological activity of the target product, simultaneously possessing antioxidative, choleretic, hepato-, radio - and nephroprotective, antiradical, wound healing effect and increase the yield of the target product, reducing energy and material costs stadiongasse aqueous extracts of cocoa-Vell.
This objective is achieved in that the extraction process cocoa-Vell is carried out in a battery of four percolation water method remuneratio with mandatory infusion with cold water, it is illustrated by the following example of a specific implementation.
In the battery of four extractors, equipped with a steam jacket and a stirrer, a load of 10 kg cocoa-Vell and 70 kg of water (20°C), mix and leave for 2 hours to swell. Then in the first extractor add 100 kg of hot (90°C) of water, heat the entire mass to 90°C and under stirring to keep the temperature from 80 to 90°C for three hours. Water extraction from the first extractor is drained and transferred to the second extractor with swollen raw material, heated to 90°C. and under stirring incubated three hours at 80-90°C. In the first extractor pour 100 kg of hot (90°C) of water, and with stirring, keep the temperature from 80 to 90°C. Similar operations are performed in all four extractors. From the fourth extractor as obtain the saturated extract. Using the fifth extractor does not lead to a significant increase in the content of extractives (table 1).
|Room extractor||The content of extractives in extracts from different extractors, kg/%|
Extract from the fourth extractor concentrated to dryness and obtain the target product with the release of 4,482 kg (44,82%). The first extractor becomes the fourth. The process learned what I continue as described above to produce the desired amount of the target product.
The proposed method for obtaining the target product in contrast to the known widen the range of pharmacological activity of the target product, to increase its output in 2 times and significantly (5-6 times) to reduce the number chamaemoro extract (instead of 500-600 kg is just 100 kg)that will significantly reduce energy and material costs.
The study choleretic activity conducted by the quantity of excreted bile. Animals of the control group were injected water. As the comparison drug used official cholagogue "Flamin". To identify the hepatoprotective activity was determined hepatoprotective index in rats to carbon tetrachloride in a toxic dose.
At the same time was determined by a percentage reduction of the enzyme alanine aminotransferase (Alat). As the comparison drug used official hepatoprotective means "Zelibor" (table 2).
Antioxidant activity was determined in accordance with the method of Reference to reduce malonic dialdehyde (MDA). Drug comparison served as vitamin E (table 2).
|Medication||Quantity of rats||Choleretic the activity, % increase||Quantity of rats||Hepatoprotective activity % reduction Alat||Quantity of rats||Antioxidant activity, % reduction of MDA|
|Target product (Cavegol)||10||94,7||10||23,3||1-0||43,5|
In table 3 the results of studies of wound healing ability after radiation damage to the skin.
|Medication||Quantity of rats||Reduction of terms of epithelialization, days /%||The starting dates of wool regrowth||Termination of the wool regrowth|
|Control (nat. R-R)||20||41,0±1,5/100||50,3±2,0||59,4±2,5|
Are given in table 2 and 3 the results of the research show that the target product (cavegol)obtained by the proposed the method, in comparison with the prototype almost does not reduce choleretic, hepatoprotective, antioxidant and wound healing (by radiation damage to the skin) activity.
Hepato - and nephroprotective activity of the target product was studied on a model of chronic endotoxemia (HAT) in rats induced by carbon tetrachloride (5 ml/kg 5 times a week), and on the sixth day of the additionally injected intraperitoneally bacterial lipopolysaccharide (Sal. typhimurium, Sigma, USA at a dose of 0.2 ml/kg) . Mortality of animals for 30 days was 10%to 90 days - 40-50%. In the comparison group (norm) used intact animals. The experimental group of animals at 7 days was received additional cavegol at a dose of 25 mg/kg orally. As the comparison drug use Essentiale, which was administered intraperitoneally in the form of a solution of 0.1 ml/kg once a day for background modeling the HAT.
In the liver histomorphometry was calculated volume fraction (ML, mm3)occupied by connective tissue, hepatocytes (%) and ML lipids, necrosis of hepatocytes (%).
In table 4 the results of morphological studies of liver tissue. It is established that the development of the HAT is accompanied by a progressive degeneration and necrosis of hepatocytes with the reaction of the microcirculation, macrophages and lymphocytes on them. Changes perisinusoidal cells and vascular reconstruction sposob the job for keeping the liver moderately expressed interstitial cytolysis, and by the 30th day revealed signs of hepatovirus. Morphometry of the experimental group of animals treated with the target product (cavegol)showed a significant limitation of all morphometric appearances secondary liver damage when CHET (table 4). Course introduction Essentiale led to partial restriction reduce the OD of hepatocytes and increase the proportion of necrotic hepatocytes, characteristic of chronic FL. At the same time increasing the OD of the connective tissue was also delayed compared with the control (table 4). Target product (cavegol) caused similar effects, comparable with Essentiale on the terms 7 and 14 days. On the 30th day of its effect on the conservation pool of hepatocytes was higher than that of Essentiale and in relation to the slow growth of connective tissue is slightly lower, but comparable with Essentiale.
|The influence of the studied drugs on the performance of the morphometry of liver, with HAT in rats|
|The experimental group||The timing of the experiment|
|7 days||21 days||30 days|
|OD connective tissue, % (normal is 1,8±0,5)|
|Target product (cavegol)||3,0±0,3*||5,4±0,6*||6,4±0,6*|
|OD hepatocytes, % (norm - 63,5±2,9)|
|Target product (cavegol)||63,1±3,7||59,2±3,7*||58,0±3,3*|
|Necrosis of hepatocytes, % (norm - 4,5±0,5)|
|Target product (cavegol)||14,0±1,1||12,5±0,8*||13,8±0,9*|
|OD lipids, % (norm -3,8±0,4)|
|Target product (cavegol)||6,0±0,7*||8,4±1,1*||9,0±1,0|
|* Significant differences from control, p≤0,05|
When assigning animals Essentiale manifestations dysmetabolic nephropathy, characteristic of chronic endotoxemia, somewhat smoothed out. The formation of nephrosclerosis was not accompanied by violations vnutripochechnykh urodynamics, the phenomena of degeneration and necrosis of the epithelium of the tubules were not as striking (table 5).
When the target product, unlike Essentiale, has been a slowing down of growth of ML connective tissue and the percentage of scleratinian glomeruli, as well as reducing the urinary space in KL the barrels. Keeping the size of the nuclei of the epithelium of the tubules, in contrast to their reduction when CHET was equally characteristic of Essentiale and kaihola.
|The influence of catechol and Essentiale on indicators of kidney morphometry with HAT in rats|
|The experimental group||The timing of the experiment|
|7 days||21 days||30 days|
|OD connective tissue, % (rate of 7.3±0,7)|
|Target product (cavegol)||8,9±0,6*||9,1±0,8*||8,4±0,7*|
|Urinary space of glomeruli (norm - 1,8±0,2)|
|Target product (cavegol)||1,9±0,2||1,7±0,2*||1,6±0,3*|
|WITH the epithelium of the tubules, μm3(norm - to 102.9±4,9)|
|Target product (cavegol)||110,4±10,1||to 102.3±9,7||the 98.9±7,8|
|Essentiale||of 101.4±9,2||of 100.2±9,5||96,4±7,7|
|Sclerotinia the glomeruli, % (normal - no)|
|Target product (cavegol)||0||0||4,4±0,4*|
|* Significant differences from control, p≤0,05|
Thus, when using kauhola able to partially restrict cytotoxic, vascular and fibroplasias mechanisms of tissue damage liver and kidneys, peculiar HAT. Its protective effects against liver comparable to the reference product, and in relation to kidney - exceed action Essentiale.
Antiradical activity (ARA) of the target product was determined by the degree of quenching of the chemiluminescence in the system, generating free radicals, using chemiluminometer HLM 1TS-01 . When you add into the system, generating free radicals, solution of the target product in volume (V) from 1 to 30 ál can determine the degree of quenching of the chemiluminescence. The initial concentration of 1 mg/ml, the solvent used water. The amount of quenching of chemiluminescence as a percentage of control X calculated according to the formula X=100%-(·100%)/K, where a is the value of damping chemiluminescence solution of a substance To suppress the chemiluminescence of solvent.
Cavegol at a concentration of 0.24 mg/ml leads to the quenching of chemiluminescence at 42.5%.
As the comparison drug used well-known antioxidant BHT (4-hydroxy-3,5-di-tert-butyl-1-methylbenzol), which is described and antiradical the I activity . Research ARA dibunola conducted under the same conditions showed no quenching of free radicals even at a concentration of 1 mg/ml
Thus, the magnitude of inhibition of chemiluminescence target product significantly exceeds the activity dibunola.
Wound healing activity of the target product burns is set to the 12 laboratory rats of both sexes at the age of 80 days. Results of experiment was used a comprehensive criterion for determining the effectiveness of the healing of burns in experimental animals.
As a first criterion was performed histological examination of burn surfaces. Sections of skin obtained at 3, 7 and 14 days of experiment. The skin samples were placed in 10% formalin solution to the posting by the standard technique and colouring with hematoxylin and eosin. This study assesses the impact of therapy on the repair processes.
The following criterion data bacteriological research in order to objectively assess the dynamics of wound healing by identifying the presence of microflora in the composition of wound and holding its qualitative assessment. The material for bacteriological analysis was 56 swabs to burn the surface of the skin of rats. As control was used 8 swabs from the burn surfaces, not p is Girgashite treatment.
Quantitative and species composition of the microflora of burn surface studied by bacteriological methods by planting swabs from the surface of the burn on differential diagnostic environment to determine the number of certain microorganisms after application of the target product and the comparison drug "Levomicol".
In the study of microbiocenosis of burn surface was taken into account: 1) the total number of microorganisms; 2) the microbes Enterobacteriaceae; 3) Staphylococcus; 4) the fungi of the genus Candida. Estimated frequency of occurrence of individual species of microorganisms depending on the biotope and products used for treatment of burns, the density of bacterial contamination in the dynamics on the 7th and 14th day of treatment of burn surface with the index of dominance, defined by the formula of Odum Yu .
The final criterion was to evaluate the dynamics of the size reduction of a skin defect: the area of the wound in percent, based on the beginning of treatment - 100% burn surface (table 6).
|The area of skin wounds (% of burn area)|
|Investigational drugs||3 days||7 days||14 days|
|Levomekol (Rev. comparison)||94,5||62,1||30,8|
|Control (without treatment)||90,0*||45,8||36,9|
|* In the early stages of animals that did not receive treatment, the surface area of the wound is reduced by 10-15%|
Moreover burn surface viewed from the position of dominance of each species as an indicator using the frequency of occurrence . It was allocated persistent species (prevalence > 50%), additional (25-30%) and casual (less than 25%).
Analysis of the microflora, allowing to divide it into a permanent and more, presented in table 7.
|The frequency of occurrence of ecological groups of microorganisms in the microbiocenosis of burn surfaces rats|
|No. biotope||Environmental groups, %|
|Staphilococcus||Streptococcus||Enterobacteriaceae||Fungi of the genus Candida|
|Target product (kaweka)||62,5||25,0||75,0||25,0|
The main representatives sown with the surface layer of burn surface, are staphylococci. Thus, staphylococci are the dominant representatives of burn surface, with the largest share among Staphylococcus belongs to the tendency of development, S.epidermidis, S.aureus (table 8).
|The degree of dominance of species of staphylococci in the community structure of burn surface|
|No. biotope||Species of staphylococci,%|
|S. saprophyticus||S. epidermidis||S. aureus|
|Target product (cavegol)||41,8||42,5||15,7|
The analysis of the dominance structure of staphylococci showed that the use of the target product prevailed saprophyte aureus representative of the resident flora. In contrast to burn the surface of the comparison group absolutely dominated Staphylococcus aureus (54,8%) and epidermal Staphylococcus with hemolytic properties. In habitats that are processed product comparison-levomekol, dominated Staphylococcus aureus (45,1%), which complicates the course of wound and burn process and causes of purulent complications.
When studying the total amount of bacteria in the dynamics found that this index was significantly decreased by 14 days of the experiment when using the target product, namely from 131, 5mm±17,1 CFU/cm2- on the 7th day of the experiment, to 100.9±12,3 CFU/cm2/sup> - on the 14th day (p<0,05). The treatment of burn surfaces product comparison levomekol not resulted in significant reduction of total bacterial contamination. The decrease in the density of microbial associations on the 14th day of the experiment was recorded on the burn surfaces that are not treated with drugs. 3 day burn all surfaces were covered with a thick scab, and some of them on day 7, moved in the third phase of healing - epithelialization, formation and reorganization of the scar.
These histological studies show that the healing of burn surface occurred during the application of both drugs, as well as in the control sample not treated with drugs. However, the rate of epithelialization was different. On the 3rd day of the experiment showing signs of epithelialization, as evidenced by basophilic staining micropreparative under a scab. The presence of cell-leukocyte infiltration actively affect the healing time and, accordingly, the rate of epithelialization. Thus, cell infiltration was slightly evident when using the target product and increased when using levamisole (drug comparison), extending the period of epithelialization. On day 7, marked clavate basophilic proliferation aimed at healing a burn on top of the spine, the most pronounced under the influence of the target product. Under the epithelial layer appears immature granulation tissue, with a rich vascularization, and growth of muscle fibers under the basal membrane. It should be noted that the use of the drug levomekol epithelialization occurs with elements of pathological regeneration, manifested in the form of growth of the epithelium around the hair follicle.
14 day registered sawtoothed epithelium with almost complete closure of the burn defect. Under kapitalizirovana plots are observed proliferation of connective tissue. The peculiarity of burn surface when applying the target product is lasting scab (until complete epithelialization), resulting in aseptic management of wounds and explains the low level of bacteriological contamination.
Thus, evaluating the degree of healing of burn wounds, you can come to the conclusion that most early epithelialization and closure of burn defect occur when using the target product (kaihola), the area of the burn and the rate of epithelialization was higher and was accompanied by the absence of purulent complications.
Thus, the inventive method allows to obtain the target product with both antioxidative, choleretic, hepato-, radio - and nephrops tectorum. antiradical activity, wound healing activity, as well as to improve the yield of the target product, to reduce energy and material costs at the stage of condensation of aqueous extracts of cocoa-Vell.
1. RF patent №2033176, CL AC 35/78.
2. Novochadov NR. Actual problems of morphology. - Tr. Siberian honey. University, Tomsk, 2002, s-106.
3. Pashkov A.N. Clinical laboratory diagnostics. M: Medicine. 5-6, 1992, pp.62-63.
4. Mashkovsky PPM Medicines. M., Ed. 14-E. LLC "New Wave", 2004.
5. Odum Yu Ecology: TRANS. from English. - M.: Mir, 1986. - 376 S.
6. Sytnik SR Environmental characteristics of the community // IMEI. - 1990. No. 9. - P.27-31.
The method of obtaining funds, possessing antioxidative, choleretic, hepato-, radio-, nephroprotective, antiradical and wound-healing effect, by infusion in water at 20°C for 2 h, followed by extraction with hot water (80-90°C) waste production confectionery industry - shredded husks of cocoa beans in the battery of four extractors, thickening of the extract and drying.
SUBSTANCE: claimed is application of "Eicosavitol", preparation, obtained by special processing of internal fat of delicatessen kinds of the Ob basin fish (whitefish, Siberian white salmon, sturgeon) as antimutagenic medication. "Eicosavitol" is unique in composition, contains up to 15% of irreplaceable higher fatty acids of "Omega 3" family, vitamins A, D, E. Also claimed is method of preventive-medicinal influence. It is shown that medication stimulates DNA synthesis 2-4 times relative to control level and causes change of DNA conformation, which appears in separation of polynucleotide chains (DNA "melting") and hyperchromism.
EFFECT: medication possesses higher efficiency.
2 cl, 5 tbl
FIELD: medicine, pharmacology.
SUBSTANCE: medication, possessing anti-inflammatory, antiseptic, spasmolytic, pain-killing and regenerating activity, represents gel and contains Dead Sea mud, hydroxyethyl cellulose, glycerin or water with the following ratio of ingredients, wt %: Dead Sea mud 45.0-70.0; hydrocellulose 1.0-3.0; glycerin or water the remaining part. Claimed gel possesses wide spectrum of antimicrobial activity, has especially positive impact on Gram-negative organisms of intestinal origin and non-spore-forming anaerobes, and also ensures prevention of infectious-inflammatory and dysbiotic diseases of vagina.
EFFECT: gel is efficient for treatment of benign diseases of uterus neck, infertility, chronic pelvic pain syndrome.
SUBSTANCE: invention refers to biotechnology, specifically to Noggin2 proteins and can be used in medicine. The signalling cascade activin is blocked by introducing Noggin2 in an organism, tissue or cell in amount effective to inhibit or decrease activin activity.
EFFECT: invention allows blocking effectively activity of protein activin.
2 dwg, 8 ex
FIELD: veterinary medicine.
SUBSTANCE: invention is related to veterinary medicine and treatment of inflammatory processes in organism. Oil-vegetable substance is applied on injured surface or introduced inside, and this prepared in the following manner. Dark glass phial is filled with the following components in certain weight ratio: pulp of sea-buckthorn without kernels, crushed dried ripe false fruit of hips, dry leaves, stems, bloom of peppermint, dry leaves, stems, bloom, fruit of killwort, dry flower cups of pot marigold, dry leaves of common plantain, dry flower cups of costmary, dry leaves of blackbutt or manna gum of Eugenia family, dry grass of touch-and-heal, dry grass of nosebleed. Vegetable mass is mixed, poured with vegetable oil, and mixture is kept with permanent mixing at room temperature up to 20 days, then 0.9% of table salt solution is poured and mixed here and left under permanent mixing at room temperature for up to 30 days, then formalin is added.
EFFECT: efficient treatment of inflammatory processes due to optimal qualitative and quantitative ratio of components in medical preparation made according to specified technology.
2 cl, 1 tbl
SUBSTANCE: invention refers to chemical-pharmaceutical industry, namely to method for making complex biologically active substances (BAS) of vegetal resources containing biologically active substances (BAS) of low-oily vegetal recourses, preferentially of wheat germs. Method involves extracting wheat germs with vegetable oil combined with disintegration of wheat germs in a rotary apparatus herewith modulating the flow passage area of the volume flow modulation depth at least 0.9 and the volume modulation depth at least 0.75 to make fine suspension of solid particle size 15 mcm or smaller. Wheat germs are pre-dried at temperature 55-65°C and mixed with vegetable oil in solid to liquid ratio within 1:2 to 1:1. An agitator is used to prepare coarse suspension of wheat germs in vegetable oil to be processed in said rotary apparatus. Made fine suspension is considered as an end-product representing vegetal-oily paste containing BAS of disperse particle size required to ensure organoleptic properties of the paste.
EFFECT: method allows producing high-quality oily product, saves the expensive vegetal resources and cost of the finished products.
5 cl, 1 dwg, 2 tbl
SUBSTANCE: invention refers to experimental medicine, particularly to experimental cardiopharmacology and can be used for endothelium dysfunction correction. For this purpose dysfunction is modelled by intraperitoneal introduction of L-nitro-arginine-methyl ester dosed 25 mg/kg daily during 7 days to rats. This therapy is combined with endogastric introduction of macrolide agent Josamycin dosed 10 and 30 mg/kg singly a day.
EFFECT: method provides correction in any antioxidant profile.
1 ex, 1 tbl
SUBSTANCE: method of augmentation of duration of action of physiologically active polypeptide in vivo is described. Physiologically active polypeptide is conjugated with Fc fragment of immunoglobulin by means of PEG. Invention use, in comparison with native physiologically active polypeptide, provides the raised physiological activity in vivo designs and-or augmentation of time of a semilife in Serum of physiologically active polypeptide with the minimum risk of induction of undesirable immune responses.
EFFECT: possibility of application of bond at manufacturing of various polypeptides medicinal preparations of the prolonged action.
11 cl, 18 dwg, 8 ex
SUBSTANCE: versions of the bond intended for linkage with the external domain B (ED-B) of a fibronectin are offered. The bond includes an antigen-binding fragment of one-chained antibody L19 and a cysteinum-containing linker for hanging of a radioactive label. Versions of a pharmaceutical composition for diagnostics and treatment of angiogenic diseases on the basis of the specified bond are opened. Application of bond for linkage with radioactive bond is described. The method of reception of bond in eucariotic cells is opened, including in Pichia pastoris and a set for reception is radioactive labelled agents on the basis of bond.
EFFECT: high-avid bond accumulation in solid tumours.
23 cl, 4 dwg, 5 tbl, 15 ex
SUBSTANCE: invention relates to veterinary and concerns treatment of inflammatory processes in animals. For this purpose oil-herbal medication is used, to prepare which mixture of crushed to amorphous powder following components is put into dark-glass bottle with ratio in g (wt %): dried leaves, stems and flowers of black mint 20.0 (1.3), dried leaves, stems, flowers and fruit of Greater celandine15.0 (1.1), dried flower heads of pot marigold 20.0 (1.3), dry flowers of German chamomile 20.0 (1.3), dry leaves of common plantain 20.0 (1.3), dried flower heads of tansy 20.0 (1.3), dry herb of common sagewort 15.0 (1.1), dry herb of St John's wort 20.0 (1.3), dry herb of common yarrow 20.0 (1.3), sea buckthorn oil - 500.0 (34.0), 0.9% sterile solution of table salt - 800.0 (54.0), formalin 10.0 (0.7). Preparation is used externally, dubbing injured part of body, or introduce internally.
EFFECT: method insures complex anti-inflammatory, pain-killing, spasmolytic, wound-healing effect.
1 tbl, 2 cl
FIELD: nanotechnologies; pharmaceutics.
SUBSTANCE: invention is related to food and pharmaceutical industry, namely to solid nanocompositions. Invention Solid nanocomposition contains 0.1-15% of active component, 40-95% of polymer, 0-6% hydrophobic or inertial polymer and 0-56% water-soluble component, at that nanocomposition on the basis contains taxipholin as active component, 40-95% of polymer, such as arabinogalactan or polyethylene glycols or polyvinyl pyrrolidones or polyvinyls of different molecular weights, and also 0-56% of water soluble polymer selected from row of possible fillers: kollidon VA64 (copolymer of vinylpyrrolidone and vinyl acetate), ludipress (modified lactose), kollidon 90F (high molecular polyvinyl pyrrolidone with molecular weight of 1000000- 1500000), sugar powder, isomaltose, 0-6% of hydrophobic or inertial polymer, where hydrophobic component contained by nanocomposition is compritol 888 ATO, (composition of mono-, di- and triglycerides of behenic acid), and inertial component is kollidon SR, Bast (mixture of polymers of polyvinyl pyrrolidone and polyvinyl acetate).
EFFECT: provides for higher bioavailability of active substances introduced in nanocomposition at creation of solid dosed forms with controlled release.
4 cl, 16 ex, 6 dwg
SUBSTANCE: invention relates to (Z)-methyl-16-(5-oxo-2-phenyl-oxazol-4-ilidenmethyl)-15,16-epoxy-8(17),13(16),14-labdatrien-18-oate of formula (I) (I). Compound (I) possesses high antioxidative, hepatoprotective and hemostimulatng activity and can be used for correction of side effects, arising with introduction of highly toxic medications used in anti-tumor therapy.
EFFECT: obtaining compound, which possesses high antioxidative, hepatoprotective and hemostimulatng activity.
1 cl, 4 tbl, 4 ex
SUBSTANCE: invention relates to acceptor of peroxide anion or nitrogen oxide, consisting of highly dispersive platinum powder, which has particle size 6 nm and less observed through microscope. Acceptor is preferably presented in form of water solution containing platinum colloid with ratio 1mM or less per 1000 ml, and can efficiently eliminate excessive level of peroxide anions or nitrogen oxide in vivo.
EFFECT: using particles of such small size allows increasing considerably efficiency of eliminating peroxide anion by means of acceptor.
2 ex, 2 tbl, 1 dwg
SUBSTANCE: dihydroquercitin in amount of 35 mg/kg is injected per os to animals with modeled peritoneal commissures for 7 days. Additionally mexydol is injected intraperitoneally in amount of 70 mg/kg. On 14th day animals are slaughtered for microscopic analysis of peritoneal commissures implications.
EFFECT: prevention of adhesion in abdominal cavity.
FIELD: medicine; pharmacology.
SUBSTANCE: invention can be used for treatment and prevention of diseases where the basic pathogenetic factor is infringement of processes peroxy and free-radical oxidations, especially lipides. As an antioxidant agent the dense extract of a grass of cowslip paigle (Primula veris L. is offered.).
EFFECT: means expands an arsenal of medical products for prevention and treatment of is free-radical pathologies.
14 dwg, 1 tbl
FIELD: medicine; biology.
SUBSTANCE: perform extraction of biologically active substances from the natural fodder additive containing (wt. %): wt. %: honey 1.5-2.0; propolisum 2.0-2.5; ambrosia 30-35; pollen load 15-17; bee sting 0.5-1.0; bee brood in different stages of development 5.5-6.0; uterine milk 3.5-4.0; bee-moth and their larvas 2.5-3.0; wax 5.0-7.0; dead bees 7.0-8.0; grass meal - the rest, by ethanol extraction within 20-21 days at 70% concentration of extracting agent, filtering extracting agent and excision of deposits, definition; solid content in supernatant fluid with the subsequent storage at temperature of 2-6°C.
EFFECT: invention allows realising the specified usage.
SUBSTANCE: present invention describes the antioxidatic composition containing, at least, one isolated improving antioxidant A with redox potential less than 180 mB, chosen of the group consisting of restored nicotinamidum-adenine-dinucleotide (NADH), recovered nicotinamidum-adenine-dinukleotidphosphate CNADPH), recovered flavinadenin-mononucleotide (FADH2), recovered flavin-mononucleotide (FMNHz), FMDH and, FADH and at least, one isolated antioxidant In, the stabilising specified antioxidant A, with standard redox potential which below standard redox potential an antioxidant A, and the antioxidant B represents a chlorophyll and-or restored ferredoxyne. Also application of the present composition is described.
EFFECT: declared invention provides stability of antioxidants at storage.
16 cl, 1 tbl, 1 dwg, 2 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention concerns medicine. A soft drink on the basis of herbs consists of the concentrated vegetative extract obtained from an admixture of herbs, chosen of Sida sps., Boerhaavia diffusa, Vitis vinifera, Tinospora cordifolia and Withania somnifera, along with green palm sugar, the agent causing a fermentation, and carbonised water, in which proportion Sida sps.: Boerhaavia diffusa: Vitis vinifera: Tinospora cordifolia: Withania somnifera in a powdery admixture makes (15-20 : (5-10 : (15-20 : (5-10 : (5-10); Proportion of wt/wt: green palm sugar: the concentrated vegetative extract makes from 1 : 3 to 1 : 4; wt/wt, a proportion of carbonised water: the admixture of the concentrated vegetative extract, green palm sugar and the agent causing a fermentation, makes from 1 : 3 to 1 : 5. Perform: (a) obtaining of parts of Sida sps., Boerhaavia diffusa, Vitis vinifera, Tinospora cordifolia and Withania somnifera; (b) crushing of parts of plants and their mixing with obtaining of a powdery admixture; (c) water addition to a powdery admixture from a stage (b) for obtaining of a water extract; (d) concentration of a water extract from the stage (c); (e) filtration of the concentrated extract from the stage (d); (f) admixture of green palm sugar to the filtered extract from the stage (e); (g) addition of Sacromyces strain and the agent causing fermentation, to the admixture from the stage (f); (h) fermentation of the admixture from the stage (g) during the period of time from 3 till 6 days; (i) filtration of the fermented admixture from the stage (h); (j) concentration of the fermented filtrate from the stage (i) with obtaining of the basic solution and (k) mixing of the basic solution (j) with the carbonised water in wt/wt proportion from 1 : 3 to 1 : 5 with obtaining of a soft drink on the basis of herbs.
EFFECT: creation of a soft drink on the basis of herbs.
11 cl, 5 tbl, 8 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention concerns the pharmaceutical, food and cosmetic industry. Perform extraction of the raw material crushed with water with obtaining of a water extract and pulp. The pulp, obtained after extraction of raw materials by the water, is extracted in two steps with ethyl alcohol. At the first step of extraction the constrictor is extracted within 4-6 hours using the following proportion: ethyl alcohol 1:(5-7) with concentration of alcohol of 30% either 50%, or 70%, at temperature of 60-75°C, obtaining an ethanolic extract of the first step of extraction and pulp; then, this pulp is repeatedly extracted within 4-6 hours with ethyl alcohol using the following proportion: ethyl alcohol 1:(3-5) with concentration of alcohol of 30% either 50%, or 70%, at temperature of 60-75°C, obtaining an ethanolic extract of the second step of extraction and pulp. After that the ethanolic extract of the first step of extraction is aggregated with the ethanolic extract of the second step of extraction.
EFFECT: increase in active substances content in an extract.
1 ex, 1 tbl
SUBSTANCE: prevention method of acute respiratory infections is developed for children with frequent recurrent diseases of respiratory tract. It consists in simalteneous administration of arbidol - 1 tablet (50 mg) twice a week during 3-4 weeks combined with selenium-active - 0.5 tablet (25 mcg of selenium) once a day during 3-4 weeks. In the course of 12 months the method helps to reduce frequency and general time duration of respiratory diseases by factor of 1.9 due to simulteneous correction of immune and metabolic state disorders.
EFFECT: reduction of frequency and general time duration of respiratory diseases.
4 tbl, 3 ex
SUBSTANCE: invention relates to medicine, namely, to experimental pharmacology. Method includes introduction of actoprotective preparation reamberinum intravenously in doze of 10 mg/kg of animal weight in morning hours 40 minutes before carrying out Porsolt tests and tests of vertical screen-net.
EFFECT: extending arsenal of means for enhancing work capacity of laboratory animals in experiment.
2 tbl, 2 ex
SUBSTANCE: invention refers to composition for prevention, symptom relief and/or treatment of skin and hair/animal fur diseases caused by inflammatory reactions, environment hostilities, ageing or cancer. Specifically the present invention concerns applications of flavonone compounds or derivatives in food, cosmetic or pharmaceutical compositions.
EFFECT: improved human or domestic animal skin and animal fur.
16 cl, 1 tbl, 5 ex, 4 dwg