Strain amycolatopsis orientalis subsp eremomycini all-russian collection of industrial microorganisms ac-807 eremomycin producent
FIELD: microbiology; medicine.
SUBSTANCE: strain Amycolatopsis orientalis subsp.eremomycini All-Russian collection of industrial microorganisms Ac-807 is eremomycin producent and can be used as source of eremomycin in medicine for treatment of severe infections.
EFFECT: increase of eremomycin output.
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The invention relates to the medical industry and relates to a new strain actinomycete forming antibacterial antibiotic eremomycin.
Antibiotic eremomycin on chemical structure relates to polycyclic antibiotics of the vancomycin group of ristomycin, which are the reserve antibiotics for treatment of severe infections.
The structure of antibiotic eremomycin sulfate
Eremomycin effective in the treatment of diseases caused by gram-positive pathogens, including metitsillinrezistentnye Staphylococcus aureus (MRSA) and gone anaerobic Clostridium difficile is the causative agent of pseudomembranous colitis.
A known strain Amycolatopsis orientalis subsp. eremomycini PMBC-S892 - producer of antibiotic eremomycin /1/adopted as a prototype. The disadvantage of this strain is a low level of biosynthetic activity is not more than 1800 mg eremomycin 1 l fermentation liquid.
The essence of the invention lies in the fact that the new strain-producer of eremomycin by mutant transformation properties of the known strain Amycolatopsis orientalis subsp. eremomycini PMBC-S892 in the sequential three-stage impact methylnitrosourea, ultraviolet and gamma rays60With the original strain and selection of the original mutant strain increased antibiotically activity.
The new strain is deposited in culture collections under the strain VKPM-As-807.
Strain Amycolatopsis orientalis subsp. eremomycini PMBC-As-807 has increased productivity eremomycin (2300 mg per 1 l of fermentation liquid) and is characterized by the following cultural-morphological and physiological traits.
Cultural and morphological characteristics of strain Amycolatopsis orientalis PMBC-As-807
Temperature 28°C; the duration of the cultivation and study of the taxonomic characteristics (7-21) days. Spore seed material with a ten-day culture of the strain grown on glukozooksidasa agar or on the environment No. 2 Gause. Color is defined according to table Bondartsev and Prauser (table 1).
Morphological features. Hyphae of the aerial mycelium straight or winding, the disintegrating elements of different lengths; the surface of spores smooth.
Physiological and biochemical properties. Aerobe. Grows at (24-37)°C; optimum growth 28°C, gelatino thins, milk iphonesimulator interterritorial hydrolyses. On the tissue does not grow. Restores nitrates to nitrites. Melanoidin pigments are not formed during growth on provocative environment Tresner with citric acid iron.
The use of carbon sources. Well utilizes fructose, arabinose, Inositol, galactose, lures, glucose; moderately lactose, rhamnose, xylose; not utilise the quote raffinose.
Antagonistic properties. Amycolatopsis orientalis subsp. eremomycini PMBC-As-807 with the growth on agar media moderately inhibits the growth of gram-positive bacteria Staphylococcus aureus, Bacillus subfilis. Bacillus mycoides, Micrococcus luteus and has no effect on gram-negative bacteria Escherichia coli, Comamonas terrigena, Providencia stuartii.
Storing strain Amycolatopsis orientalis PMBC-As-807
Is stored in the form of culture, dried in sharethelove environment in apalach if (5-7)°C.
Antibiotic activity of strain Amucolatopsis orientalis PMBC-As-807
is determined in the culture fluid by the method of diffusion in agar (trehgogny option GFH, str) using as the test organism Bacillus subtilis 6633.
Strain Amycolatopsis orientalis PMBC-As-807 is illustrated by the following example.
Example 1. Spore material with mycelium culture Amycolatopsis orientalis PMBC-As-807 grown within (10-14) days at 28°C in the environment Lindenbaum, inoculated nutrient medium (150 ml Erlenmeyer flasks 750 ml) and grown at 28°C for 24-36 hours on a rocking chair at (220-240) rpm
The composition of the seed medium, %:
|On the bacilli chloride||0,3|
|pH before sterilization||7,0-7,2|
The medium is sterilized for 30 min at a pressure of 0.6 ATM at 120°C.
Grown mycelium in the amount of (5-10)% of inoculated nutrient medium (150 ml Erlenmeyer flasks 750 ml) of the following composition,%:
|Magnesium sulfuric acid||0,01|
|pH before sterilization||6,8-7,0|
The fermentation is carried out at 28°C for (120-144) hours on a rocking chair at (220-240) Rev/min Eremomycin accumulated in the culture fluid of a producer. Antibiotic activity is here determined by the method of diffusion in agar with the use of test-cultures of Bacillus subtilis ATCC 6633. The content of eremomycin in the culture fluid of strain Amycolatopsis orientalis PMBC-As-807 to the end of the fermentation is 2300 mg/ml
With the aim of identifying eremomycin conduct its sorption from native solution (filtered from mycelium culture fluid) on carboxylic cation exchanger KB-2 is in salt form, elute with aqueous ammonia, and the eluate is neutralized, obezbolivaet is passed through the sorbent PTFE, an antibiotic with a sorbent displace water, concentrated in vacuo to content eremomycin (120-140) mg/ml and crystallized in the form of sulfate.
Eremomycin sulfate, obtained by biosynthesis new strain - producer Amycolatopsis orientalis PMBC-As-807, is a white crystalline powder, easily soluble in water and insoluble in organic solvents. Indicators eremomycin sulfate (physico-chemical properties, antibacterial activity and toxicity) fully complies with the indicators defined for eremomycin sulfate, protected by the RF patent №2110578 (Brutto-formula: C73H89N10C1O26×H2SO4. Mol. weight 1656 u
UV spectrum: λmax(0,01 N. HCl) nm (E%1 cm) 280 (42); λmax(0,01 N. NaOH) nm (E10,0cm) 300 (60);
IR-spectrum (KBr), νmaxcm-1: 3350, 1650,1500, 1200-1000; [α]20D=-100°(1,0, H2O).
The yield of the target product is cleaned is about (97% purity) of crystalline drug eremomycin sulfate content in the native solution is (60,0-65,0)%.
Thus, created a new mutant strain of Amycolatopsis orientalis PMBC-As-807, which differ from the known character of the growth diagnostic agar media, degree of assimilation of carbon sources (Inositol, galactose) and by the ability of antibioticaccutane, which is 78% larger than the activity of the strain prototype Amycolatopsis orientalis PMBC-S892 (table 2).
Antibiotic activity of a new strain of Amycolatopsis orientalis PMBC-As-807 is 2300 µg/ml Using a new strain of Amycolatopsis orientalis PMBC-As-807 as producer for the biosynthesis of eremomycin allows to increase the yield of the target product. The output eremomycin is 1.2 grams per 1 liter native solution.
Sources of information
Patent RU 2110578 C1 from 18.06.97
|Cultural and morphological characteristics of strain Amycolatopsis orientalis PMBC-As-807|
|Agar medium||The growth of the air mycelium*||The color of the mycelium||Soluble pigment|
|No. 1 Gause||No||Colorless||No|
|Oatmeal||+ -||White (D-1)||Colorless||No|
|Of čapek||No||White (D-1)||Colorless||Yellow|
|Glucosamina||+ -||From white (D-1) to yellowish (W-1)||Yellow (G-6)||Yellow (G-6)|
|Lindenbaum (glycerol-nitrate)||+ +||White (D-1),||Colorless||Yellow (G-6)|
|No. 2 Gause||+ -|
|Tresner (peptone and citric acid iron)||Melanoidin pigments are missing|
Strain Amycolatopsis orientalis PMBC-As-807 - producer of antibiotic eremomycin.
FIELD: chemistry, biochemistry.
SUBSTANCE: invention refers to biotechnology and agriculture, specifically to fungi growing. Interstrain hybridisation method is applied to produce somatic strain of macroscopic oyster fungus Pleurotus ostreatus (Fr.) Kumm "ВИК" F - 6 being a producer for carposomes of pileate fly edible fungi of carposome productivity 60-70% of dry weight of substratum and high commercial properties. Strain is deposited in "ВКМ" under number F - 3889 D.
EFFECT: higher productivity and improved carposome habitus.
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FIELD: chemistry; mining.
SUBSTANCE: strain Acidithiobacillus ferrooxidans IB 12, which possesses high capability of extracting copper ions, can be used for bioleaching waste of floatation concentration sulfide ore.
EFFECT: increase in efficiency of bioleaching copper from wastes of enriching polymetallic sulfide ore.
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FIELD: chemistry; biochemistry.
SUBSTANCE: present invention pertains to microbiology, biotechnology and agriculture. Presented here is a strain of Escherichia coli "BioR.Prolyzer-4L" (B-9843), which produces lysine and is meant for feeding monogastric animals and birds, with the aim of boosting their fertility. The invention also relates to the "Prolyzer-BioR" additive, which contains this strain, deposited on a carrier. Description is given of a method of feeding monogastric animals and birds, involving putting additives into the feed, which is a culture of this strain, grown in a medium using Hottinger's broth at 25°C-37°C for a period of 6-12 hours until attaining 5*109-32*109 per 1 l content of living microbe cells.
EFFECT: increased value of aminoacid feed of monogastric animals and birds, lower cost of the feed, and consequently, price of meat of monogastric animals and birds.
13 cl, 16 tbl, 3 ex
FIELD: chemistry, biochemistry.
SUBSTANCE: invention relates to biotechnology, particularly to medical microbiology and can be used to isolate the brucellosis. Nutrient medium contains a dense phase and a liquid phase. Dense phase contains refined sugar, sodium chloride, sodium phosphate di-substituted, yeast extract microbiological agar and pipe water in the following ratio. Liquid phase contains nutrient bullion for culturing microorganisms dry, sodium citrate, glucose and distilled water in the following ratio.
EFFECT: cutting of the time for isolating the brucellosis microbe.
FIELD: chemistry; biochemistry.
SUBSTANCE: present invention pertains to biotechnology. The actinomycete strain Streptomyces violaceus "ВКПМ" Ac-1734 -producer of glycosidase inhibitor can be used as a producer of glycosidase inhibitor in microbiological production of glycosidase inhibitor for the food, chemical-pharmaceutical industry, biotechnology and biochemistry.
EFFECT: increased output of glycosidase inhibitor.
FIELD: chemistry; biochemistry.
SUBSTANCE: proposed invention pertains to biotechnology and can be used for carrying out ecological toxic tests when monitoring water ecosystems. The Vibrio fischeri "ВКПМ" B-9580 strain, with high sensitivity to effect of toxic substances with very diverse chemical nature, can be used in testing toxic levels of sea-floor sediments.
EFFECT: increased sensitivity of the test-culture to effect of toxic substances of very diverse chemical nature.
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FIELD: chemistry, biochemistry.
SUBSTANCE: offered invention refers to biotechnology and can be used for ecological toxic analysis and aquatic ecosystem monitoring. Strain Vibrio fischeri "ВКПМ" B-9579 highly sensitive to multichemical toxic substances can be used in toxicity testing of bottom sediments.
EFFECT: higher sensitivity of test-cultures to multichemical toxic substances.
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SUBSTANCE: method includes preplant treatment of seeds by application of the composition on their surface containing Heteroauxin and a chitosan-containing preparation, obtained from the myxomycet biomass of Saprolegnia parasitica on the set technology, in the weight ratio 3:400 at their total consumption 161.2 g/t.
EFFECT: possesses greater efficiency against root rot at reduced consumption of pesticides.
FIELD: medicine; veterinary science and biotechnologies.
SUBSTANCE: select assays of the biological material. Then carry out inoculation of these assays on the medium Endo and incubate in a thermostat within 18-24 hours. Then carry out inoculation on sorbitol - an agar and incubate within 18-24 hours. Then in addition carry out incubations on the SDS broth with the subsequent analysis of results.
EFFECT: improvement of quality of research of a biological material, reduction of duration of researches and simplification of the method.
SUBSTANCE: said utility invention relates to the biotechnology and agriculture namely to the protection of plants against phytopathogenic fungi and bacteria. The strain Bacillus subtilis B-14 is isolated from soil and deposited in the Microorganism Culture Collection Research Institute of the State Centre for Virusology and Biotechnology VECTOR under the registration number B-1149. The strain demonstrates antagonistic properties against a broad spectrum of phytopathogenic fungi and bacteria, is undemanding as far as nutrient media are concerned, and may be used for the protection of various plant types against diseases.
EFFECT: obtaining a bacteria strain demonstrating antagonistic properties against a broad spectrum of phytopathogenic fungi and bacteria, and undemanding to nutrient media.
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SUBSTANCE: invention relates to technology of glycopeptides purification. Clarified liquid containing eremomycin is compacted with sulphuretted stirol-divinylbenzene cationite, elution is carried out and neutralised eluate is applied on the column with hydrophobic stirol divinylbenzene sorbent. After washing column with water, eremomycin is displaced with solution of isopropyl alcohol with addition of acetic acid and crystallisation of target product is carried out.
EFFECT: obtaining a pure product with a high output at a high process productivity.
6 cl, 3 ex
FIELD: food industry, confectionary industry.
SUBSTANCE: for the purpose to obtain jelly marmalade with a wide spectrum of vitamin activity and unconventional pleasant combination of organoleptic properties one should prepare the syrup by applying sugar, pectin and an aqueous extract of the mixture of baikhovi black tea, baikhovi green tea, Centaurea flowers, calendula flowers, mountain cranberry leaves, hibiscus flowers and dog rose fruit. While preparing it is necessary to supplement the syrup with citric acid, sodium lactate, "Mountain cranberry" and "Cream" aromatizing agents and a preparation obtained out of Mortierella exigua micromycete biomass by the preset technique to be further spread, formed, dried and packed as ready-to-use products.
EFFECT: higher efficiency.
FIELD: canned food industry.
SUBSTANCE: method involves preparing fruits; adding sugar solution; providing cavitational grinding of fruits in sugar solution and separating pulp under aseptic conditions; providing cavitational grinding by dispersing under atmospheric pressure in mixture of fruits and sugar solution of mycelium produced after extracting of Mortierella zychae micromycet biomass with non-polar liquefied gas; sequentially extracting biomass with water, alkaline, water, acid, water, alkaline, and water; introducing resultant solid residue into sugar solution in an amount of about 0.06% by weight of fruits.
EFFECT: reduced consumption of power for producing of canned food, improved quality of canned food owing to improved retention of color of basic material, and increased resistance to breaking of canned food during storage.
FIELD: biotechnology, microbiology.
SUBSTANCE: invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.
EFFECT: improved preparing method.
15 cl, 4 ex
FIELD: chemistry; biotechnologies.
SUBSTANCE: method involves sampling from oily soils and earth, shedding consortium of native oil-oxidising microorganisms, herewith followed with activating consortium of native oil-oxidising microorganisms with mixed biotrine and alcohol waste distillers in ratio 1:1 in amount at least 0.01 wt %.
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