Method of infectious keratitis treatment
SUBSTANCE: invention concerns ophthalmology and is intended for treatment of infectious diseases of a cornea. Photodynamic therapy with the use of gel containing an octacation phthalocyanine of zinc in concentration of 1-3 mg/ml, which is applied on a cornea as a photosensitinogen, is performed. The conjunctival cavity is washed out after 30 - 40 minutes and influenced with a laser radiation with a wavelength of 675 nm with a light dose 20 - 60 J/cm2 in of 5 minutes, once a day. 1-8 sessions during each course.
EFFECT: method allows destroying the originator of keratitis in a cell with the maximum safety of surrounding tissues of the cornea.
2 tbl, 3 dwg, 1 ex
The present invention relates to ophthalmology and is intended for the treatment of infectious diseases of the cornea.
According to epidemiological analysis, patients with inflammatory lesions of the eyes occupy the first place among applicants on outpatient reception (40,2%), accounting in the Russian Federation, about 16 million people per year [Yuzhakov A.M. "the Main directions in the elimination of avoidable blindness in the Russian Federation" Ophthalmology 2003; 44(4), 6, S.; Michuk Û.F. corneal Ulcer // Ophthalmologist. - 1999, №4. P.9-14]. With inflammatory diseases linked up to 80% of cases of temporary disability, up to 50% of hospitalizations, and 30% of blindness. To date estimated for Russia among the total number of patients with inflammatory diseases of the eye keratitis accounts for 4.2% of the patients. Corneal blindness is one of the first three causes of sudden vision loss [Michuk Û.F. "Principles of the world who initiatives for the elimination of avoidable blindness. Possible ways of their use in the Russian Federation". Ophthalmology 2003; 44(4), 7 C.].
Severe ulcerative keratitis and persistent corneal opacity in the outcome of diseases and injuries of 10% lead to blindness. Among the serious diseases of suppurative corneal ulcers account for 17% [Starkov GL, Sokolova R.S. epidemiology, prevention and screening ophthalmologic patients with external inflammatory diseases Vestn. oftalmol. - 1988, No. 1. volume 104 C.3-5]. In the treatment of bacterial keratitis prescribe broad-spectrum antibiotics in the form of drops, ointments and medical films. In severe ulcerative lesions of the cornea, which are often aureus and Pseudomonas aeruginosa, antibiotics enter subkonyunktivalno, intramuscularly or intravenously. Difficulties in the treatment of microbial infections of the cornea cause changed in recent years, the nature of the microflora and increase its virulence [Keratitis and erosion. Edited Lckoseao, Apostolova, Iagora "GEOTAR-Media", 2006 page 27]. Separately, the problem is the treatment of keratomycosis (keratitis, caused by a fungal infection). Keratomycosis have a tendency to chronicity and some resistance even to the most modern methods of treatment [Walsh, TJ. (1987) Recent advances in the treatment of systemic fungal infections Methods Find Exp Clin Pharmacol 9, 769-778]. Thus, despite the availability of a wide spectrum of antibacterial drugs, the percentage of severe outcomes suppurative keratitis is still high. This is due to the presence of antibiotic resistance of pathogenic microorganisms, the development of toxic and allergic reactions to their introduction, dysbiosis and indigocarminata, suppression of the immune system.
The closest analogue of the present invention is a method for the same purpose is, includes treatment of the affected area of the cornea aqueous solution of sodium hypochlorite [the Feasibility and clinical efficacy of sodium hypochlorite in the complex treatment of patients with extremely severe suppurative keratitis. V.v.egorov, Gposnova, Elaraby, Ioualalen. Clinical ophthalmology. 2005, volume 6, str-156]. According to the authors, the drug is a broad-spectrum antiseptic and manifests bactericidal effect against gram-positive, gram-negative organisms and fungi. However, the method is not without disadvantages.
1. Systemic and local routes of administration of sodium hypochlorite have some significant drawbacks. These include: toxic effects on the cornea, the violation of its natural protective film, conjunctival Allergy, pain, trauma, irritation of eye medications.
2. In addition, instalatii sodium hypochlorite do not provide the necessary therapeutic concentrations of drugs in the affected area.
3. Sodium hypochlorite, despite the wide range of actions, which are not antibiotics, had only a bactericidal effect and is unable to penetrate the microbial cell.
4. Necroticism action of sodium hypochlorite can have a negative impact on healthy tissue of the cornea, p is Evodia to the dimness of the stroma. The objective of the invention was to develop a highly effective treatment of infectious keratitis various etiologies with minimal risk of complications.
The technical result of the invention is the complete elimination of the causative agent of keratitis in its various forms directly inside the cell while the maximum preservation of the tissue of the cornea.
The technical result is achieved due to the photodynamic effect with the use of a photosensitizer (PS) gel content actication phthalocyanine zinc applied to the entire surface of the cornea.
Photodynamic therapy (PDT) has been successfully applied in various fields of medicine: in Oncology, in ophthalmology for the treatment of age-related macular degeneration, etc. In recent years in foreign literature began to appear in publications about the use of PDT in the treatment of local skin infectious diseases [Soukos N.S. Targeted antimicrobial photochemotherapy, Antimicrob Agents Chemoterapy, 1998, 42, 2595-601 Zak O. Animal infection models - the perfect infection model, J Antimicrob Chemother. 1993, 31, 193-205]. Of great importance is the fact that unlike chemotherapy multiple potential targets for oxidative degradation (primarily structural proteins, enzyme systems, unsaturated fatty acid residues of lipids) in antimicrobial photodynamic therapy inhibits p is the development of cellular resistance. We investigated the efficacy of photoinactivation using cationic photosensitizers cultured gram-negative bacteria Campylobacter jejuni, Helicobacter pylori, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. In accordance with the descending order of effectiveness of photoinactivation in the processing of 2 μg/ml of Holocene and 5 j/cm2red light is built the following way: gram(+) Staphylococcus aureus > gram(-) microaerophile Helicobacter pylori ≥ Campylobacter jejuni > gram(-) aerobes Escherichia coli > Pseudomonas aeruginosa. It is shown that the highest value of the minimum inhibitory concentration actication phthalocyanine zinc (1.7 µg/ml, about 1 μm) is observed for Pseudomonas aeruginosa.
However, in the treatment of infectious keratitis PDT has not been previously used. Considering literature data, the task was to use the possibilities of PDT using actication phthalocyanine zinc in the treatment of infectious keratitis. Because the cornea has its own structural features (sphericity, transparency) and washed by tears, we used actication phthalocyanine zinc in gel form to increase the contact time of the drug with the surface of the cornea. For this purpose, our research has found different proportions gel photosensitizer. Carried out researches have allowed to find the optimal concentration of FCS in the gel and the optimal parameters of laser irradiation.
After application of the photosensitizer in the mix with the gel on the cornea initiated photodynamic effect of laser radiation with a wavelength of 675 nm and a light dose of 20-60 j/cm2. As our investigations have shown, the light dose is less than 20 j/cm2insufficient to initiate a photodynamic effect, and more than 60 j/cm2may cause damage to the corneal stroma. To achieve the optimal therapeutic effect of the number of sessions was chosen depending on the extent of damage to the cornea. The larger size of the lesion, the greater the number of sessions. Sessions of photodynamic therapy (PDT) is conducted once a day for no more than 8 days.
We experiment the treatment of keratitis mild severity.
Rabbits with the generated model of keratitis mild severity is divided into groups:
A - control group, not receiving antifungal therapy;
B - group receiving conventional medical treatment: in the instillation of Amphotericin b × 4 times, ointment Ketoconazole × 2 times a day;
In experimental group, receiving PDT.
All animal groups on the cornea applied the gel content actication phthalocyanine zinc at a dose of 2 mg/ml over the next 30 minutes is the interaction of a photosensitizer (PS) with pathogenic microflora. Then the cornea is washed from excess FS 09% solution of sodium chloride. The next stage laser effect. For PDT, the used laser system for photodynamic therapy LFT-630/675-01 "BIOSPEC" GOST R 50460-92. A light dose of 40 j/cm2when the duration of the session 5 minutes.
In animals served as control (group a)developed bilateral endophthalmitis. Histological examination revealed the melting of the cornea, its investirovanie by leukocytes, eosinophils, necrotic damage to the stroma, the appearance of newly formed vessels. Sharp dilation and the plethora of vessels, the formation of a fibrinous exudation into a single conglomerate characterized picture of endophthalmitis. In stroma half-sections, each drug were found budding yeast fungi. This fact was mentioned by activity infectious process.
The results of the treatment of keratomycosis rabbit after PDT were compared with those obtained with traditional treatment. The evaluation criteria were the terms of the clinical and microbiological disappearance purulent discharge, corneal syndrome, epithelization of the cornea and attenuation of the inflammatory response.
The results of experimenal presented in table 1. After carrying out of conservative treatment on histological sections were marked hyperplasia of the epithelium, intra - and su the epithelial edema, the increase in the number of keratocytes, fungal colonies enclosed in fibrinous case (figure 2), indicating that the encapsulation of the pathogen and low effectiveness of therapy. After PDT, the pathogen was not detected in any of the examined eye. It was noted complete epithelialization of the corneal defect with transparency stroma. In the result we can say that the PDT of keratomycosis more effectively compared with the standard antifungal therapy. Also, work was performed to study the efficacy of PDT in the treatment of keratomycosis moderate severity.
A - control group, not receiving antifungal therapy;
B - group receiving conventional medical treatment: in the instillation of Amphotericin b×4 times, ointment Ketoconazole × 2 times a day;
In experimental group, receiving PDT. As in the treatment of keratitis mild severity, PDT keratomycosis more effectively compared with the standard antifungal therapy. The results of the experiments are presented in table 2.
All animals in the group And developed clinical and histological picture of endophthalmitis. On histological sections (Figure 1) shows the melting of the cornea, its investirovanie by leukocytes, eosinophils, necrotic damage stroma (stained with hematoxilin-EA is different, magnification × 60).
From complications of ulcers in the subgroup (B) observed only the posterior synechia in 16% of cases. Analysis of the results of treatment according to the degree of recovery of corneal transparency showed that surface avascular turbidity was formed in 75% of cases, and gentle slovakukrainian 25%, while in the second subgroup (In) in addition to the posterior synechia 66% in one eye (13%) detected descemetocele. Gentle slovakukrainian opacities in the outcome of the keratitis was observed in 66%.
The dynamics of the pathological process in the first group (in days, M±m).
|The parameters||Subgroup B (n=3)||Subgroup (n=3)|
|The timing of the disappearance of pus||2,00±0,17||3,27±0,30|
|The timing of the disappearance pericorneal injections||to 3.73±0,24||4,12±0,43|
The speaker is a pathological process in the second group (in days, M±m).
|The parameters||Subgroup B (n=3)||Subgroup (n=3)|
|The timing of the disappearance of pus||5,00±0,41||6,37±10,23|
|The timing of the disappearance pericorneal injections||11,00±0,46||13,15±0,46|
|Resorption of hypopyon||7,01±0,32||8,64±0,21|
The rabbit with the generated model of keratitis moderate severity on the cornea applied the gel content actication phthalocyanine zinc at a dose of 2 mg/ml over the next 30 minutes is the interaction of a photosensitizer (PS) with pathogenic microflora. Then the cornea is washed from the surplus of the Federal Assembly of 0.9% solution of sodium chloride. The next stage was carried out by laser irradiation. For PDT, the used laser system for photodynamic therapy LFT-630/675-01 "BIOSPEC". A light dose of 40 j/cm2when the duration of the session - 5 minutes. The treatment was performed 1 time per day should the 3 days. On day 7 was produced histological examination of the obtained materials.
On histological sections revealed a moderate increase in the density of keratocytes in superficial stromal layers, the absence of inflammatory cells, a small zone of lysis in the stroma (figure 3), which proves the high efficiency of therapy.
Because the rabbit is the optimal model for reconstruction and development schemes of treatment of keratitis, we can say that the developed method can be successfully extrapolated into clinical practice. Thus, our proposed method for the treatment of keratitis is quite effective and safe for the surrounding tissue and can be used in daily ophthalmic practice.
The method of treatment of infectious keratitis, characterized in that the conducting photodynamic therapy using as a photosensitizer gel containing actication phthalocyanine zinc at a concentration of 1-3 mg/ml, which is applied to the cornea after 30-40 min, washed into the conjunctival cavity and irradiated by laser radiation with a wavelength of 675 nm with a light dose of 20-60 j/cm2duration : 5 min, once a day, at a rate of 1-8 sessions.
SUBSTANCE: invention concerns medicine, namely, physiotherapy, reflexotherapy. Prior to the beginning of backbone extension acupuncture by the rule "Mother-son" is performed. In 3-5 days the backbone is simultaneously influenced with a magnetic-laser radiation and extension is performed. The magnetic-laser radiation influence is spent in red 630-650 nm and infra-red 890-950 nm ranges with 50 mT intensity of a magnetic field. Backbone extension is spent in mass of a load by 15-30 kg. Total time of the procedure makes 15-20 minutes.
EFFECT: method increases analgetic effect, reduces puffiness and improves microcirculation and trophicity of tissues.
SUBSTANCE: invention relates to field of veterinary. Method includes preliminary feeding medical herb flour to cattle on the background of main diet. After feeding with medical herb flour, cattle is subjected to single low-intensity magnet-laser exposure with semi-conductor laser "Isel-Victoria" on the region of spine from withers to tail attachment from both sides. Exposure is performed for 5-10 minutes from each side, for cows from distance 10-12 cm, for calves from distance 3-4 cm from middle sagittal line. Exposure is performed with the following radiation modes: wave length 0.85-0.89 mcm, laser impulse power 3.5 mW -5.0 W, pulse repetition frequency -50-80 Hz, energy dose - 0.002-0.024 J/cm2, power density - 0.1-0.3 mW/cm2 .
EFFECT: method allows to remove heavy metals from cattle organism in short time.
SUBSTANCE: invention relates to medicine, namely to psychotherapy, reflexotherapy and concerns treating patients suffering from compulsive overeating. Psychotherapeutic influence is realised by means of individual suggestive influence. Patient is brought into the state of trance and simultaneously their acupuncture points are influenced, before individual suggestive influence, neurolinguistic programming is carried out and new ecological image of "healthy Ego" is formed. Essence transformations of personality are realised and new healthy principles of correct nutrition are formed, prevention of "diet depression" is also realised, individual suggestive influence being directed to psychological support, distraction from persistent thoughts about food, weight and figure, normalisation of food intake, elimination of diet self-restrictions, solution of life difficulties; reflexotherapeutic influence on acupuncture points is realised by infrared photobiostimulation and extremely high-frequency exposure onto corporal points, after which patient is given home task in form of "diary of correct food behavior". Infrared photobiostimulation is performed on point T20, extremely high-frequency exposure is performed on corporal points E36, MC6, GL4, VB8, VC12, RP6. Individual suggestive influence is performed by means of Ericksonian hypnosis. On finishing the course of treatment, psychological support is carried out in form of consultations in order to form and support strategy of correct food behaviour. In process of carrying out reflexotherapy course simultaneously infrared exposure of low intensity and extremely high-frequency exposure are used. Individual suggestive influence, infrared exposure of low intensity and extremely high-frequency exposure are carried out simultaneously.
EFFECT: method lies in increasing treatment efficiency.
SUBSTANCE: simultaneous joint physiotherapeutic influence repeating during several days by a magnetic field, infra-red laser radiation and radiation of light-emitting diodes on area of a postoperative wound is performed. Physiotherapeutic influence is carried out starting from the second day after operation, within 10 days, twice a day during morning and evening time, with a therapy break for the fourth day. And from the first till the third day laser radiation is influenced at frequency of repetition of impulses of 5 Hz, powers of radiation 30 mW and duration of 6 minutes. From the fifth till the tenth day laser radiation at frequency of repetition of impulses from 50 Hz to 600 Hz, power of continuous radiation is influenced of the light-emitting diodes 60 mW and duration of influence of 5 minutes.
EFFECT: acceleration of an angenesis of patients after operation, analgesic effect, absence of purulent complications, possibility to refuse a fixing thoracal corset.
FIELD: medicine; surgery.
SUBSTANCE: for treatment of keloid or hypertrophic cicatrixes of a skin unitary freeze a cicatrical tissue on a site in diameter 1 the exposition of 15-20 seconds. Thus rate of handpiece cooling makes 220°C/minute. Temperature on a working part of the handpiece makes 180°C. Then, without waiting of thawing, a laser photocoagulation in a pulsed operation is carried out. Frequency of a pulsed operation makes 10-12 Hz, power 5 W. All area of cicatrix is treated in the same way.
EFFECT: rising of efficiency of treatment, reduction of number of relapses, treatment depreciation, reduction of terms of treatment, maintenance of painlessness of procedure.
SUBSTANCE: invention concerns medicine, particularly cardiology, and can be applied in coronary disease treatment, exertional angina of I-II functional class. Magnet laser therapy is performed starting from 4th adaptation day. Infrared laser radiation is applied with wavelength of 0.89 mcm in impulse mode of intensity of 7 W, pulse frequency of 80 Hz and constant magnetic field with induction of 50 mTl. Each of 4-5 symmetrical bioactive points is affected for 1 minute. Total impact time is 8-10 minutes. Full-body hydrosulfide baths are added starting from 5th day of treatment with hydrosulfide concentration of 50-150 mg/l for exertional angina of I functional class and with hydrosulfide concentration of 50-100 mg/l for exertional angina of II functional class. Water temperature is 36°C, duration comprises 6-15 minutes. Baths are performed on days free from magnet laser therapy. A course includes 8 physiological procedures and 8 balneological procedures.
EFFECT: enhanced physical efficiency, stimulated activity of involuntary nervous system, improved indicator values of lipid exchange, peroxide lipid oxidation, antioxidant and coagulation systems, reduced risk of coronary disease complications.
2 ex, 14 tbl, 3 dwg
SUBSTANCE: correction of pain syndrome accompanying severe psoriasis is ensured with skin irradiation within affected region or above inflammatory tissue using low intensity IR rays of CO2 laser. Laser wavelength is 10.6 mcm. Radiation power density in pulse-periodic regime is 0.015-1 Wt/cm2, radiation pulse duration is 0.5-10 mc, pulse window is 10-100 mc, and highly directional IR radiation divergence is 10-2 radians. While radiation, laser beam is moved on skin within affected region during 5-10 minutes, herewith zone exposure duration determined by laser beam diameter is 1-2 sec. Skin is preliminary coated with adenosine triphosphate with daily exposure within course of 10-15 days.
EFFECT: evident reparative analgesic effect, reduced time of hospital stay, prevention of complications.
FIELD: medicine; physiotherapy.
SUBSTANCE: ultrasonic inhalations of 10 ml solution of 1% placenta hydrolyzate are applied at temperature of solution 35°C within 10 minutes. Procedure is applied in the morning on an empty stomach, or 40-60 minutes after eating. It is followed with scanning laser therapy with infra-red laser radiation from laser scanning physiotherapeutic stimulator LSPS-03.2IR. Output power at light guide end is 2 mWt, power flux density is 50 mWt/cm2. While exposure the patient is positioned lying on stomach, thus effecting paravertebral fields to the right and to the left at level D3-D9, within 3 minutes per each field. Then while the patient is positioned lying on back, liver region is exposure within 8 minutes. Course is 10 daily procedures.
EFFECT: normalised eliminative properties of epithelial barrier of a respiratory tract and local immunity of airway mucous membrane.
3 tbl, 2 ex
FIELD: medicine; dentistry.
SUBSTANCE: treatment of inferior dental nerve neuritis caused by filling material delivery into mandibular canal is ensured with introduction of antibacterial agent before operation. Thereafter filling material is surgically removed from mandibular canal. Operation procedure is followed with introduction of antibacterial and immune correcting agents, antisensitisers, Reaferone, vitamins B1 and B12, adenosine triphosphate. Besides mandibular region is exposure to laser magnetotherapy with using dental apparatus Optodan, accompanied with intraoral HBO-therapy, hydromassage, hydrocortisone phonophoresis, 2% nicotinic acid solution amplipulse phoresis, Tambukan mud therapy, pine needle and pearl baths, acupuncture with using local and regional points.
EFFECT: higher treatment efficiency, restored blood circulation, accelerated rehabilitation, complete relief of evident pain syndrome and post-amputation pains, simplicity, efficiency.
FIELD: medicine; pulmonology.
SUBSTANCE: intravenous laser irradiation of blood (ILIB) is carried out differentially depending on detected hemodynamic type of microcirculation and level of protein C system activity. In case of hyperemic, spastic and normocirculatory hemodynamic type of microcirculation and activity level of protein C system of 0.704±0.02 and higher, 7 sessions of ILIB are performed. In case of stasis and stagnant hemodynamic type of microcirculation and activity level of protein C system less than 0.704±0.02, 12 sessions of intravenous laser irradiation of blood. Length of wave is 0.63 mcm, intensity of irradiation at light guide end makes 1.5-2.0 mW, one procedure per day, duration makes 20 minutes.
EFFECT: lower demand in antibacterial therapy and in medicines of pathogenetic action, lower medicamental load on patient and reduction of disease clinical symptoms preservation duration.
5 tbl, 2 ex
SUBSTANCE: admixture of 10 ml 0.35% Radachlorine solution in 200 ml of 0.9% isotonic sodium chloride solution is prepareed for treatment of bladder inflammation (cystitis). Then the admixture is entered under the ultrasonic control into a bladder cavity through a catheter in a dose by of 50-200 ml on increasing depending on physiological volume of a bladder and sensation of fullness. Empty the bladder in 30-60 minutes. After that irradiate with a monochromatic light of 650-670 nanometers wavelength and density of energy 200 J/cm2. Influence is carried out in a continuous regimen of radiation incutaneous manner with two fields on pubic and sacral areas. An exposition on each area makes 25-30 minutes. Course of treatment makes 5-15 sessions with 1-2 days interval. As an irradiation source it is possible to use the ELAN complex with a wavelength of radiation of a matrix of 650-670 nanometers, density of power of working radiation of a matrix in a focal plane 30-60 mW/cm2 and the area of maculae irradiation in a focal plane of 40 cm2.
EFFECT: reduction of terms of treatment, prevention of allergic reactions and resistance of a microflora to antibiotics and medicinal preparations, the prevention of development of dysplastic and cicatrical changes in a bladder mucous.
2 cl, 2 ex
FIELD: medicine; pharmacology.
SUBSTANCE: substance of technical decision seeks to produce immunopotentiating composition based on nanotechnology products, specifically fullerenes and superdispersed diamond powders (nanodiamonds) produced by detonation synthesis. Composition contains modified fullerene of general formula C60-X, where C60 is fullerene nucleus, X is formylporphyrin, as well as superdispersed diamond powder in ratio as follows, wt %: porphyrin modified fullerene C60 - 50; superdispersed diamond powder - 50.
EFFECT: synergetic effect of immunopotentiating components activity with simultaneous considerable reduction in price concerning prototype composition.
2 tbl, 1 dwg
FIELD: medicine; gynaecology.
SUBSTANCE: solution of photosensitiser "Photoditazine" is introduced intravenously in dose of 0.5-1.0 mg/kg. As soon as 1.5-2 hours have elapsed after infusion, intrauterine light guide is inserted into uterine cavity, and uterine cavity is radiated with laser in fractions at wave length of 662 nm and density of supplied energy of 200 - 350 J/cm2 for 30-45 minutes. After uterine cavity has been radiated, cervical channel is radiated in fractions by laser with application of cylindrical light guide at wave length of 662 nm and density of supplied energy of 200-250 J/cm2 for 15-30 minutes.
EFFECT: highly selective removal of functional and basal layer of uterus mucous layer.
SUBSTANCE: PS is chlorophyll derivatives. PS is activated by light with oxygen, preliminary or in one stage introduced to organism using wave energy sources. Herewith radiation power is 0.1-20000 Wt, with dose 1-100000 J.
EFFECT: higher specificity of antibody response, suppression of pathological centre, activation of cell exchange processes, higher immune protection of organism in whole, improvement of life quality and tumour operability.
3 cl, 11 dwg, 1 tbl, 3 ex
FIELD: medicine; ophthalmology.
SUBSTANCE: intravenously administer FS of chlorine family in a dose of weight of 0.8-1 mg/kg. One hour prior to transpupillary laser irradiation enter driply 50% of all doses within 10 minutes. The remained 50% of a dose administer 10 minutes prior to irradiation. Then transpupillary irradiate all surface of angiomatous formation with laser radiation concentrically from the centre to periphery. Energy density at irradiation totals 100-120 J/cm2 in the centre on one field and 50-80 J/cm2 on periphery. Irradiation is spent with overlapping of the next fields on 15-20% of the area. For the second day intravitreal enter antiangiogenic preparations: avastine - 1.25 mg or Kenalogum - 4 mg.
EFFECT: full resorption of angiomatous formation, prevention of relapses in the remote postoperative period.
2 cl, 1 ex
FIELD: medicine; oncology.
SUBSTANCE: tumour and metastasises site diagnostics is preliminary carried out using hardware and software complex "Sozvezdie". Then photosensitiser chlorine e6 is introduced. After that tumour is exposure with of laser wavelength 660±20 nm during exhalation and heart diastole. Pulse repetition rate is 22.5±2 kHz, pulse duration is 70±30 ns, and power density is 0.1-0.5 Wt/cm2. Thus retention of tumour reheat temperature is controlled within 42-45°C. Method can be applied after operation and/or chemotherapy and/or radiation therapy.
EFFECT: accelerated rehabilitation and higher selectivity of laser exposure and increase of cellular immunity.
4 cl, 3 ex
SUBSTANCE: as photosensibilizers emulsions of alkylthiosubstituted phalocyanides of general formula , where R1=R4=Cl, R2=R3=n-C10H21S, M=Zn or R1=t-C4H9S, R2=R3=R4=H, M=HH or R1=t-C4H9S, R2=R4=H, R3=t-C4H9, M=HH or R1=R3=t-C4H9S, R2=R4=H, M=HH or R1=R2=R3=R4=t-C4H9S, M=HH or R1=R4=Cl, R2=R3=t-C4H9S, M=Zn, in water solution of Proxanol 268 are suggested.
EFFECT: elaboration of highly selective and efficient photosensibilizers for application in photodynamic therapy of tumours.
3 cl, 18 ex, 4 tbl, 9 dwg
SUBSTANCE: invention is referred to the medicine, dermatology, and can be used for treatment of patients with psoriasis. For this purpose perform complex medical therapy with application of hyposensitising, antihistamine, disintoxication agents, hepatoprotectors, and vitamin preparations. In addition perform photodynamic therapy. As a photosensitinogen, administer preparation Photostim in the form of syrup. Thus administer Photostim from 3rd day from the beginning of treatment in the morning before meals in a dose of 5 ml daily within 30 days. With a wavelength of 650 nanometers and power 50 mW on cm2 with perform exposure by light radiation from the 7th day and before the end of Photostim administering.
EFFECT: photosensitinogen dose decrease, excluding phototoxic and allergic reactions.
SUBSTANCE: method is performed by the following way: unfiltered culture liquid is acidated to pH 2-2.5, sorb on cationite KU-23/100 in pulsing fluidisated mode with subsequent desorption using elution systems with pH 6.0±0.1, containing sodium citrate 1.5% and carbamide 0.5%. Coproporphyrin III is precipitaled on produced eluate, reducing pH to 3.5±0.1. Produced precipitate is extracted with hydrochloric acid solution, thereafter chromatographic cleaning of extract on molecular sorbent follows. End product is precipitaled on eluate. Then extraction and chromatographic cleaning are repeated, whereupon end product is precipitaled on eluate and transformed into potassium salt.
EFFECT: technology allows for production of water-soluble preparation based unfiltered culture liquid.
FIELD: medicine; ophthalmology.
SUBSTANCE: intravenously enter a photosensitinogen of a chlorine number in a dose of 0.5 mg/kg within 10 minutes. Simultaneously with it perform intravenous laser irradiation of blood in cubital vein of other arm. Thus the wavelength corresponds to a maximum of "ФС" absorption of light radiation of 50 mW power. Then transpupillary irradiate the centre with one laser emission maculae with the same wavelength and density of energy 15 J/cm2.
EFFECT: method allows to reach a selective obliteration of neogenic vessels of choroidal neovascular membranes at the minimum damaging influence on intact structures of a retina.
SUBSTANCE: invention concerns medical equipment, in particular devices for orientation blind in surrounding space, and can be used at independent movement blind in non determined situation, and also in extreme situations when blind urgent medical aid is required. The cane represents a stick with the handle with a receiver-radiator, established on the cane with the possibility of change of an angle of an inclination to a stick axis in planes of symmetry of a cane, the block of processing of the information, the power supply and an annunciator. The receiver-radiator it is supplied by an agent of an operational control of an angle of its inclination to an axis of the stick in the course of movement of the user. In addition there is a transmitter with the radiating aerial, an including button, the pendular switch, the relay and the receiver with the catching aerial. The transmitter is executed in the form of consistently included a setting high-frequency generator, the phase manipulator which second input is bridged to an exit of the generator of pseudo-random series, and the power amplifier, which exit the receiver - in the form of consistently connected to the catching aerial of the amplifier of high frequency, a line of a delay and the phase detector which second input is bridged to an exit of the amplifier of high frequency is connected to the radiating aerial, and, and the exit is connected to the block of registration and an alarm signal annunciator.
EFFECT: invention use allows dilating functionality of the cane for the sight-disabled person by transfer of alarm signal to rescuers, physicians or relatives in case of extreme situation on the radio channel.