Method of modelling of postmedicamental immunodeficiency

FIELD: medicine.

SUBSTANCE: invention concerns experimental medicine and can be used for modelling of an immunodeficiency, characteristic for oncologic patients. For what the compelled swimming of animals in water is carried out at temperature 18°-20°C for 15-20 minutes - within 3 days daily. Then next day a single intraperitoneal injection of Cyclophosphanum of 8-10 mg is spent counting on 100 g weight of a body of an animal to 1.0 ml of a solution and research of immunologic indicators for 5-6 day after an injection.

EFFECT: provision of adequate reproduction of model by the most approached to a real situation at the expense of preliminary stress activation.

1 tbl, 4 ex

 

There is a method of modeling postmedication immunodeficiency (Microwave millimeter range in the prevention of experimental postmedication immunodeficiency cytotoxic Genesis. Auth. Niemierko and other Topical issues of rehabilitation medicine, balneology and physiotherapy: Mater. International Congress "Health 2006. - M., 2006). The way the prototype is to initiate immunodeficiency States by a single intraperitoneal injection of 0.5 ml of cytostatic cyclophosphamide at a dose of 4-8 mg/100 g of body weight of the animal. This method cannot be considered fully adequate real clinical situations, when many cancer patients chemotherapy runs in the background of another no less important factor syndrome - stress.

The method of the invention is much closer to these situations. The essence of it is that at first, in experimental animals, for example rats, cause stress. This process is carried out by forced swimming animals in tap water at a temperature of 18°-20°15-20 minutes for 3 days a week. The next day produced an injection of cyclophosphamide at a dose of 8-10 mg/100 g of animal body weight intraperitoneally. On day 5 after injection performed in vivo immunological studies of leukocytes and Lenovo the mule blood, phagocytosis by neutrophils by Ivanov and Tuchlovice (the method of determining the absorptive and digestive ability of neutrophils. - Lab. case. - 1967. - 10. - 610-613), mass of the spleen and thymus.

The method is as follows - first initiate model of stress by forced swimming animals in drinking water at a temperature of 18°-20°15-20 minutes for 3 days a week, on the following day to produce a single intraperitoneal injection of 8-10 mg of cyclophosphamide per 100 g of body weight of the animal in 1.0 ml. At 5-6 days after injection examined immunological parameters.

To study the adequacy and severity of the proposed model is studied 31 rats male Wistar rats weighing 200-250 g were divided into 4 groups. The first group was represented by 7 intact animals. 7 rats of the second group received an injection of cyclophosphamide at a dose of 8-10 mg/100 g body weight; 8 animals 3 groups initiated stress by forced swimming animals in drinking water at a temperature of 18°-20°15-20 minutes for 3 days a week); group 4 - rats, which were first initiated stress with the aforementioned method, and then they received an injection of cyclophosphamide (9).

At 5-6 days after injection was made the study of the immunological tests.

Table data strongly suggest that is the result of exposure by the method of the invention develop reliable immunosuppressive shifts in the ratio of the masses of immunocompetent organs, and the main index of phagocytosis - FAL (phagocytic activity of leukocytes), PHIL (phagocytic index of leukocytes) and NRF (the rate of completion of phagocytosis).

The influence of the developed model and its components on the indicators of immunity
IndexGroupN g.NMmP
Weight of spleen (mgThe intact17134596,21-2<0.012-3<0.01
t/f 1,027898100,11-3>0.12-4>0.05
stress381475,1150,41-4>0.63-4>0.2
Stress + C/f 1.0491250136,5
Thymus mgThe intact19248,324,21-2<0.0012-3<0.01
t/f 1,027101,618,8 1-3>0.12-4>0.1
stress38of 199, 924,51-4<0.013-4>0.05
Stress + C/f 1.049143,418,4
FAL, %The intact1947,81,931-2<0.0012-3>0.05
t/f 1,02723,42,171-3<0.0012-4>0.8
stress3818,801,001-4<0.0013-4<0.05
Stress + C/f 1.04922,91,38
PHIL, unitsThe intact190,980,091-2<0.0012-3>0.05
t/f 1,0270,390,081-3<0.0012-4>0.3
stress380,230,021-4<0.0013-4>0.2
Stress + C/f 1.049 0,300,05
NRF, %The intact1926,71,451-2<0.0012-3>0.3
t/f 1,0275,862,421-3<0.0012-4>0.5
stress3810,50the 3.651-4<0.0013-4>0.5
Stress + C/f 1.0498,032,78

The method can be illustrated by the following examples.

1. The intact rat male Wistar rats No. 1, weight 210, Additional impacts not received. The animal study carried out simultaneously with the animals of the experimental group (on day 5 after injection of cyclophosphamide). The weight of the spleen 1200 mg, thymus 243 mg, AOK spleen 104 Cells/PE, FAL 54%, PHIL 1,56 unit, NRF 28,2%.

2. Rat male Wistar rats No. 23, weight 210 g received an injection of a solution of platinum in the above dose. The animal study carried out on day 5 after injection. The weight of the spleen 570 mg, thymus 64 mg, AOK spleen 136 CL/PE, FAL 26%, PHIL 0,28%, NRF 7,14%.

3. Rat male Wistar rats No. 13, weight 230 g, which is achala was initiated stress with the aforementioned method, and then she got an injection of cyclophosphamide in the above dose. The animal study carried out on day 5 after injection. The weight of the spleen 970 mg, thymus 86 mg, AOK spleen 120 CL/PE, FAL 24%, PHIL 0,36%, NRF 11,1%.

4. Rat male Wistar rats No. 14, weight 250 g, which was first initiated stress with the aforementioned method, and then she got an injection of cyclophosphamide in the above dose. The animal study carried out on day 5 after injection. The weight of the spleen 1130 mg, thymus 140 mg, AOK spleen 160 CL/PE, FAL 24%, PHIL 0,26%, NRF to 7.6%.

The way of modelling the immunodeficiency characteristic of cancer patients, including the forced swimming animals in tap water at a temperature of 18°-20°15-20 minutes for 3 days a week, then the next day a single intraperitoneal injection of 8-10 mg of cyclophosphamide per 100 g of body weight of the animal in 1.0 ml of a solution and investigation of the immunological parameters at 5-6 days after injection.



 

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